CN110301896B - Device and method capable of synchronously acquiring biological tissue structure image and blood flow image - Google Patents
Device and method capable of synchronously acquiring biological tissue structure image and blood flow image Download PDFInfo
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Abstract
The invention relates to a device and a method for synchronously acquiring a biological tissue structure image and a blood flow image, wherein the device comprises a light source mechanism, an optical imaging mechanism and a control analysis system; the light source mechanism comprises a laser light source and an incoherent visible light source; the optical imaging mechanism comprises an optical imaging lens group, an optical filter switcher and a photoelectric imaging device which are sequentially arranged along the light detection path, wherein a visible light optical filter and a laser optical filter are embedded in the optical filter switcher, and a visible light filtering channel and a laser filtering channel are respectively formed; the control analysis system is connected with the photoelectric imaging device and used for analyzing the acquired biological tissue structure image and blood flow image. The device capable of synchronously acquiring the biological tissue structure image and the blood flow image is convenient for accurately fusing the blood flow image and the structure image of the biological tissue, and is convenient for rapidly realizing comprehensive observation analysis of physiological processes such as blood flow of the biological tissue, distribution and change of vessel diameter and the like.
Description
Technical Field
The invention relates to a device and a method for synchronously acquiring a biological tissue structure image and a blood flow image.
Background
The laser speckle blood flow imaging technology is a novel optical imaging technology capable of rapidly acquiring blood flow distribution and blood flow change of biological tissues, has the advantages of non-contact, no need of contrast agent, no need of scanning and full-field imaging, and is widely popularized and applied in the field of life science in recent years.
In addition, a rapid laser speckle blood flow imaging system and method disclosed in application No. 201310234441.3, a quantitative imaging device for laser speckle blood flow velocity disclosed in application No. 201510937070.4, a laser speckle blood flow imaging device of application No. 201520414558.4, and the like are continuously explored and innovated in terms of resolution, precision, application portability, and the like.
However, the existing laser speckle blood flow imaging device cannot synchronously acquire structural image information and blood flow image function information of biological tissues, so that comprehensive analysis of physiological processes is inconvenient to quickly realize.
Disclosure of Invention
Based on the above, it is necessary to provide a device and a method capable of synchronously acquiring a biological tissue structure image and a blood flow image, so that the blood flow image and the structure image can be fused accurately, and comprehensive observation analysis on physiological processes such as blood flow of biological tissues, distribution and change of vessel diameters and the like can be realized conveniently and rapidly.
The technical scheme for solving the technical problems is as follows:
a device capable of synchronously acquiring biological tissue structure images and blood flow images comprises a light source mechanism and an optical imaging mechanism;
the light source mechanism comprises a laser light source and an incoherent visible light source, and the laser light source and the incoherent visible light source are obliquely arranged at a preset inclination angle;
the optical imaging mechanism comprises an optical imaging lens group, an optical filter switcher and a photoelectric imaging device which are sequentially arranged along the direction of the light detection path, wherein a laser optical filter and a visible light optical filter are embedded in the optical filter switcher, and a laser filtering channel and a visible light filtering channel are respectively formed;
when the device is used for imaging biological tissues, the laser light source, the laser filtering channel and the photoelectric imaging device are matched to obtain blood flow images of the biological tissues, and the incoherent visible light source, the visible light filtering channel and the photoelectric imaging device are matched to obtain structural images of the biological tissues.
Further, the device capable of synchronously acquiring the biological tissue structure image and the blood flow image further comprises a light homogenizing device, wherein the light homogenizing device is arranged at the front end of the laser light source, so that the problem that the divergence angle of the semiconductor laser is inconsistent with the length and the short axis of the emergent light spot and the light spot is uneven is further solved, and the imaging effect is improved.
Preferably, the light homogenizing device is a cylindrical mirror, a diffusion sheet or ground glass.
In one embodiment, the light source mechanism further comprises a constant temperature chamber and a constant temperature and constant current controller, the laser light source is a semiconductor laser, the semiconductor laser is arranged in the constant temperature chamber, and the constant temperature chamber is connected with the constant temperature and constant current controller, so that consistency of light emitting effect is ensured.
Preferably, the laser light source is a 785nm semiconductor laser, and the laser filter is a 785nm bandpass filter.
Preferably, the incoherent visible light source is an LED light source, for example, a monochromatic LED light source or a broad spectrum white LED light source, and the wavelength range is in the visible light band.
In one embodiment, the incoherent visible light sources are arranged in an annular shape, and the incoherent visible light sources and the laser light sources are coaxially arranged, so that the volume of the device is further reduced.
In one embodiment, the optical imaging mechanism comprises a stereoscopic microscope having the filter switch embedded therein. The stereoscopic microscope with the embedded double-filter switcher is convenient for realizing microscopic imaging of the fine structure of biological tissues.
Preferably, the optoelectronic imaging device is an area array digital CCD camera, an area array digital CCD video camera, an area array digital CMOS camera or an area array CMOS digital video camera, and the number of analog-to-digital conversion bits is not lower than 12.
A method for synchronously acquiring biological tissue structure images and blood flow images adopts the device, and comprises the following steps:
placing a biological tissue sample to be detected, lighting an incoherent visible light source, switching to a visible light filter, focusing to be clear, and obtaining a structural image of the biological tissue;
and (5) lighting the laser light source, switching to the laser filter, and obtaining a blood flow image of the biological tissue.
The beneficial effects of the invention are as follows:
the invention can synchronously acquire the structural image and the blood flow image of the biological tissue through the matching arrangement of the light source mechanism comprising the laser light source and the incoherent visible light source, the optical imaging mechanism comprising the optical filter switcher and the photoelectric imaging device and the like, is convenient for realizing the accurate fusion of the blood flow image and the structural image at the pixel level on one hand, is convenient for observing and quantitatively analyzing the functional information related to physiological response such as the blood flow distribution, the blood flow change, the vessel diameter contraction and expansion and the like of the biological tissue on the other hand, does not need to adjust the imaging visual angle, and is rapid in operation.
Drawings
FIG. 1 is a schematic diagram of an apparatus for simultaneously acquiring biological tissue structure imaging and blood flow imaging according to an embodiment;
FIG. 2 is a schematic diagram of another embodiment of an apparatus for simultaneous acquisition of biological tissue structure imaging and blood flow imaging;
FIG. 3 is a schematic diagram of the method steps for synchronously acquiring imaging of biological tissue structures and blood flow imaging according to the present invention.
In the drawings, the list of components represented by the various numbers is as follows:
110. the system comprises a laser light source 120, an incoherent visible light source 200, an optical imaging mechanism 210, an optical imaging lens group 220, a filter switcher 230, a photoelectric imaging device 300 and a control analysis system.
Detailed Description
The principles and features of the present invention are described below with reference to the drawings, the examples are illustrated for the purpose of illustrating the invention and are not to be construed as limiting the scope of the invention. It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Referring to fig. 1, an apparatus for synchronously acquiring an image of a biological tissue structure and an image of a blood flow according to an embodiment includes a light source mechanism, an optical imaging mechanism 200, and a control analysis system 300.
In the present embodiment, the light source mechanism includes a laser light source 110 and an incoherent visible light source 120.
The laser source 110 is preferably a 785nm semiconductor laser, is a near infrared light source, has a linewidth stability of less than + -0.02 nm, and can be a 785nm bandpass filter.
Further, the light source mechanism also comprises a constant temperature chamber and a constant temperature and constant current controller. The semiconductor laser is arranged in the constant temperature chamber, the constant temperature chamber is connected with a constant temperature constant current (driving current) controller, a temperature monitoring element, a heating and cooling element and the like in the constant temperature chamber are respectively connected with the constant temperature controller, constant temperature regulation and control are realized, and consistency and stability of blood flow imaging are improved.
The incoherent visible light source 120 preferably employs an LED light source, such as a monochromatic LED light source or a broad spectrum white LED light source, with a wavelength in the visible light band, preferably 400-650 nm.
In this embodiment, the laser light source 110 and the incoherent visible light source 120 are disposed in a relatively inclined manner, so that the incoherent visible light source 120 and the laser light source 110 can irradiate an observed object at a set inclination angle (for example, 55 °), and when a biological tissue sample to be detected (observed object) is focused clearly, the irradiation center of the light source coincides with the center of the field of view, thereby avoiding the process of adjusting the angle of view.
Referring to fig. 2, in other embodiments, the incoherent visible light sources 120 are preferably arranged in an annular shape, and the incoherent visible light sources 120 and the laser light sources 110 may also be arranged in a coaxial inclined manner, so as to simplify the volume of the device.
By enabling the laser light source 110 and the incoherent visible light source 120 to be obliquely arranged at a preset inclination angle, the laser light source 110 and the incoherent visible light source 120 can conveniently irradiate an observed object at the preset inclination angle, when a biological tissue sample to be detected (the observed object) is clearly focused, the irradiation center of the light source is exactly coincident with the center of the visual field, the process of adjusting the visual angle can be avoided, and the imaging efficiency is improved.
Further, the device for synchronously acquiring the biological tissue structure image and the blood flow image in this embodiment further includes a light homogenizing device disposed at the front end of the laser source 110, so as to synchronously solve the problem of uneven light spots caused by inconsistent divergence angles of the semiconductor laser and the length and the short axis of the emergent light spots. The light homogenizing device is preferably a cylindrical mirror. For example, a cylindrical mirror with f=50 may be used at a certain distance in front of the laser light source 110. The light-homogenizing device is not necessarily limited to a cylindrical mirror, and may be a device having a light-homogenizing effect such as a diffusion sheet or frosted glass.
In the present embodiment, the optical imaging mechanism 200 includes an optical imaging lens group 210, a filter switcher 220, and a photo-electric imaging device 230, which are disposed in this order in the light detection path direction. The optical imaging lens group 210 is used for amplifying and focusing the biological tissue sample to be detected. The filter switcher 220 adopts a dual filter switcher (IRCUT) loaded with two filters, and a visible light filter and a laser filter are embedded therein, and a visible light filter channel and a laser filter channel are formed. Preferably, the double-filter switcher adopts an electric control switching mode, so that the serial port communication protocol is conveniently used for controlling the coordination of the light source lighting and IRCUT switching by means of the control analysis system, and the imaging mode switching efficiency is improved. Incoherent visible light source 120, a visible light filtering channel (e.g., a visible light filtering channel of 400-650nm is used) and photo-electric imaging device 230 are combined to obtain a structural image of biological tissue. The laser light source 110, the laser filter channel and the photo-imaging device 230 cooperate to acquire a blood flow image of the biological tissue.
Preferably, the optical imaging mechanism comprises a stereoscopic microscope, the embedded dual filter switch being within the stereoscopic microscope. At this time, the laser light source 110 and the incoherent visible light source 120 may be disposed at an inclination of 55 ° according to the working distance of the stereoscopic microscope. The adoption of a stereoscopic microscope is convenient for microscopic imaging of the biological tissue fine structure.
Preferably, the optoelectronic imaging device adopts an area array digital CCD camera, an area array digital CMOS camera or an area array CMOS digital camera, and the number of analog-to-digital conversion bits is not lower than 12.
In the present embodiment, the control analysis system 300 is connected to the photo-electric imaging device 230 for analyzing the acquired structural image and blood flow image of the biological tissue. Preferably, the control analysis system 300 can also directly control the switching cooperation of the light source mechanism and the filter switch 220.
With further reference to fig. 3, the method for imaging by using the device capable of synchronously acquiring the biological tissue structure image and the blood flow image according to the present embodiment includes the following steps:
(1) Placing a biological tissue sample to be detected, opening the device to preview the image, and at the moment, illuminating the laser source 110 to select an imaging mode.
(2) When the structural imaging mode is selected, the incoherent visible light source 120 is lightened, the visible light filtering channel is switched to obtain a structural image of the biological tissue, and the structural image is transmitted to the control analysis system 300.
When the blood flow imaging mode is selected, the incoherent visible light source 120 is turned off, the laser filtering channel is switched, a laser speckle blood flow calculation algorithm is adopted, a blood flow image of the biological tissue is acquired, and the blood flow image is transmitted to the control analysis system 300.
(3) And (3) repeatedly selecting the steps (1) and (2) to image in different modes, or repeatedly performing the steps (1) and (2) to image the structural image and the blood flow image of the next tissue sample to be detected.
(4) And (3) turning off all the light sources after the imaging is finished.
In the device capable of synchronously acquiring the biological tissue structure image and the blood flow image in the embodiment, the irradiation modes of the two light sources are designed and assembled according to the specific inclination angle, so that the whole imaging process is not required to be regulated, and the illumination modes of the two light sources (the laser light sources are lightened for a long time and the incoherent visible light sources are timely lightened) and the light imaging lens group and the double-filter switcher are matched, so that the imaging is not mutually interfered and is not interfered by ambient light. When a structural image of biological tissue is acquired, back scattered light is collected by an optical imaging mechanism and imaged on a photoelectric imaging device through a visible light filtering channel; when blood flow images of biological tissues are acquired, back scattered laser is collected by an optical imaging mechanism and imaged on a photoelectric imaging device through a laser filtering channel (near infrared filtering channel); the photoelectric imaging device transmits the acquired image to a control analysis system (module) for calculation and analysis.
The device capable of synchronously acquiring the biological tissue structure image and the blood flow image is convenient for realizing the accurate fusion of the blood flow image and the structure image at the pixel level on one hand, and is convenient for observing and quantitatively analyzing the functional information related to physiological response such as blood flow distribution, blood flow change, blood vessel diameter contraction and expansion of the biological tissue on the other hand, and is rapid in operation.
The technical features of the above embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (4)
1. The device capable of synchronously acquiring the biological tissue structure image and the blood flow image is characterized by comprising a light source mechanism and an optical imaging mechanism;
the light source mechanism comprises a laser light source, an incoherent visible light source, a light homogenizing device, a constant temperature chamber and a constant temperature constant current controller, wherein the laser light source and the incoherent visible light source are obliquely arranged at a preset inclination angle of 55 degrees, the irradiation mode of the laser light source and the incoherent visible light source is designed and built at the preset inclination angle of 55 degrees without adjustment in the whole imaging process, the light homogenizing device is arranged at the front end of the laser light source, the incoherent visible light source is a 400-650nm LED light source, the incoherent visible light source is annularly arranged, and the incoherent visible light source and the laser light source are coaxially arranged; the laser light source is a 785nm semiconductor laser, the semiconductor laser is arranged in the constant temperature cavity, and the constant temperature cavity is connected with the constant temperature constant current controller;
the optical imaging mechanism comprises an optical imaging lens group, an optical filter switcher and a photoelectric imaging device which are sequentially arranged along the direction of a light detection path, wherein the optical imaging lens group is used for amplifying and focusing biological tissues to be detected, a laser optical filter and a visible light optical filter are embedded in the optical filter switcher, a laser filtering channel and a visible light filtering channel are respectively formed, the optical imaging mechanism comprises a stereoscopic microscope, and the optical filter switcher is embedded in the stereoscopic microscope;
when the device is used for imaging biological tissues, the laser light source is turned on for a long time, the incoherent visible light source is turned on immediately, the laser light source, the laser light filtering channel and the photoelectric imaging device are matched to obtain blood flow images of the biological tissues, the incoherent visible light source, the visible light filtering channel and the photoelectric imaging device are matched to obtain structural images of the biological tissues, and the blood flow images and the structural images are fused at pixel level.
2. The apparatus for synchronously acquiring the image of the biological tissue structure and the image of the blood flow according to claim 1, wherein the light homogenizing device is a cylindrical mirror, a diffusion sheet or ground glass.
3. The apparatus for synchronously acquiring the biological tissue structure image and the blood flow image according to claim 1 or 2, wherein the photoelectric imaging device is an area array digital CCD camera, an area array digital CMOS camera or an area array CMOS digital camera, and the number of analog-to-digital conversion bits is not lower than 12.
4. A method for simultaneously acquiring images of biological tissue structures and blood flow, characterized in that it comprises the following steps, using a device according to any one of claims 1 to 3:
placing a biological tissue sample to be detected, lighting an incoherent visible light source, switching to a visible light filtering channel, focusing to be clear, and obtaining a structural image of the biological tissue;
the laser light source is lightened, the laser light source is switched to the laser filtering channel, and a blood flow image of the biological tissue is obtained;
and the blood flow image and the structural image are fused at the pixel level.
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| CN107510430A (en) * | 2017-09-23 | 2017-12-26 | 武汉迅微光电技术有限公司 | Endoscopic optical imaging method and system a kind of while that obtain otherwise visible light color image and blood-stream image |
| CN109752377A (en) * | 2019-02-02 | 2019-05-14 | 佛山科学技术学院 | A kind of beam splitting type bimodal projection chromatography tissue blood vessel imaging device and method |
| CN209048125U (en) * | 2017-08-28 | 2019-07-02 | 佛山科学技术学院 | A kind of vascular flow projection chromatography 3-D imaging system |
| CN211131004U (en) * | 2019-07-03 | 2020-07-31 | 武汉迅微光电技术有限公司 | Device capable of synchronously acquiring biological tissue structure image and blood flow image |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106725349A (en) * | 2017-02-28 | 2017-05-31 | 武汉迅微光电技术有限公司 | A kind of dermoscopy of detectable blood flow |
| CN209048125U (en) * | 2017-08-28 | 2019-07-02 | 佛山科学技术学院 | A kind of vascular flow projection chromatography 3-D imaging system |
| CN107510430A (en) * | 2017-09-23 | 2017-12-26 | 武汉迅微光电技术有限公司 | Endoscopic optical imaging method and system a kind of while that obtain otherwise visible light color image and blood-stream image |
| CN109752377A (en) * | 2019-02-02 | 2019-05-14 | 佛山科学技术学院 | A kind of beam splitting type bimodal projection chromatography tissue blood vessel imaging device and method |
| CN211131004U (en) * | 2019-07-03 | 2020-07-31 | 武汉迅微光电技术有限公司 | Device capable of synchronously acquiring biological tissue structure image and blood flow image |
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