CN110301559A - Test adult feedstuff and its application of red crucian carp - Google Patents
Test adult feedstuff and its application of red crucian carp Download PDFInfo
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- CN110301559A CN110301559A CN201910721225.9A CN201910721225A CN110301559A CN 110301559 A CN110301559 A CN 110301559A CN 201910721225 A CN201910721225 A CN 201910721225A CN 110301559 A CN110301559 A CN 110301559A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The present invention relates to fish culture fields, in particular to test red crucian carp adult feedstuff and its application.The adult feedstuff is in terms of mass percentage, crude protein content >=30%, crude fat content >=5%, crude fiber content≤6%, crude ash content≤12%, total phosphorus content >=1.0%.The feed has filled up domestic market blank.With the red crucian carp adult feedstuff of experiment made of this feed formula, nutrition is balanced, and palatability is good, and feed conversion rate is also higher；Both it had been able to satisfy the nutritional need for testing red crucian carp normal growth and normally breed lived in aquarium, can also reach control requirement of the Laboratory Animal Standardization management process to feeding quality；Not only nontoxic, harmless to red crucian carp is tested, but also aquarium aquatic environment is not polluted.
Description
Technical field
The present invention relates to fish culture fields, in particular to test adult feedstuff and its application of red crucian carp.
Background technique
Testing red crucian carp is a kind of emerging native country Model fish in China, has important application value.Testing red crucian carp is water
Raw poikilotherm tests red crucian carp suitable for water environment and tames by red crucian carp, is a kind of aquatic poikilotherm, is 0 DEG C in temperature
Can survive in~39 DEG C of water body, and lower oxygen concentration resistance (O.lmg/L), in barrier environment the water temperature of aquarium can be controlled in 18 DEG C~
24 DEG C, it is applicable to the zoopery of water environment, the advantage with Aquatic Ecotoxicology research and environmental safety evaluation.
Testing red crucian carp has the morphological feature for being adapted to live in water ecological environment, and figure suitably tests red crucian carp in spindle figure, body
Side is flat, full squama, mouth end position, without single tail is similar to carp.The dorsal fin fin for testing red crucian carp C1HD system (a kind of inbred strais) is D. I
~II -16~19, anal fin fin is A. I -5~7, and pectoral fin fin is P. I -14~17, and abdomeinal fin fin is V. I -7~8, lateral line scales
Scale formula is.A length of 100~the 150mm of adult body.The figure for testing this size of red crucian carp is well suited for testing, it can also be anti-
Living body is carried out again and takes blood, and disposable extraction blood volume will not be lethal up to 1.0~1.5mL.Such as it is suitble to do heavy metal, chemicals life
Object enrichment experiment, convenient for taking the tissues such as liver, kidney to be analyzed.Testing red crucian carp has more perfect tract and physiological function,
Similar mammal.Red crucian carp is tested with more perfect digestive system, the circulatory system, excretory system, nervous system, endocrine system
System, reproductive system etc., the structure and function of tract are similar to mammal.What it is different from mammal is exhaled with the gill
It inhales, heart is 1 atrium, 1 ventricle, and red blood cell has nucleus, the platelet-free with thrombocyte.Testing red crucian carp has in vitro
Oviposition, ovum diameter big (680~720 μm), in vitro fertilization, ectogenetic development characteristics, are suitable for doing gene transfer, development biology
It learns and reproductive biology is studied.The gill can be used as membrane physiology research model；M cell (Mauthner cell) can be used as research maincenter
The model of nervous function；Mast cell (mast cell, MC) can be used for immunological investigation；Modified Retinal Model can be constructed to grind
Study carefully optic nerve physiology；Reproducible liver tumor model, for carrying out oncology studies etc..
It is clear to test red crucian carp genetic background, is standardized Fish as Laboratory Animal.Test red crucian carp chromosome number 2n=
100, karyotype formulas 22m+30sm+24st+24t, full-length genome size are 2.16Gb, and number of genes is 44580.Relative to
The red blood cell DNA content of chicken is 2.5pg, and the red blood cell DNA content of red crucian carp is 3.0pg, minimum in cultivation Strains of Carassius auratus, is shown
Red crucian carp has the advantages that model organism.Testing red crucian carp C1HD system is a kind of pure lines derived from gynogenesis, principal biological number
Mark (such as RAPD, SSR, AFLP, SNP are marked) it has been established that suitable for carrying out genome walking, turning according to central genetic
The research such as gene studies, genetic analysis and sexual control.
The oviposition for testing red crucian carp is more, and reproductive capacity is strong, in liberal supply.1~2 age tested red crucian carp, and just sexal maturity, fish brood amount reach
1.5~80,000, primary oviposition also up to 30,000 can several times a year lay eggs, therefore reproductive capacity is strong, ample supply of commodities on the market.
The body colour for testing red crucian carp is entirely red, and watches and be integrated with experiment.The mutation that red crucian carp is crucian carp is tested, and goldfish close relative,
Its body colour is complete red gorgeous, is both the excellent instruction of a kind of fresh water fancy fishes and Drinking Water and water resource pollution monitoring
Biology, it may also be used for genetics research, such as the research of color inheritance, tail fin character variation.Its viability is strong, is easy to Shui nationality cage breeding
Growing the red crucian carp of experiment is omnivorousness, and viability is stronger, and raising is easy in aquarium, can also be developed up to sexal maturity.It feeds and manually matches
Feed is closed, similar breeding goldfish is equally raised, handy.It is suitable for aquarium cultivation and experimental study.
It is domestic at present to appear on the market not yet dedicated for the red crucian carp perfect compound feed commodity of raising experiment.The reality cultivated indoors
It tests in red crucian carp aquaculture, in the scientific experimentation that the red crucian carp of application experiment is carried out, lacks the standardized red crucian carp full price of experiment of one kind and match
Close feed, seriously affect the red crucian carp of experiment normally produce it is accurate with scientific experimentation result.Therefore, special in testing red crucian carp aquaculture
It is not to provide a kind of standardization during carrying out scientific research and environmental safety evaluation as experimental material to test red crucian carp
The red crucian carp perfect compound feed of experiment have important practical significance.
Summary of the invention
In view of this, the present invention provides a kind of adult feedstuff for testing red crucian carp and its application.The feed both ensure that experiment
The nutritional need of red crucian carp normal growth and breeding in aquarium, and can have to red crucian carp production and zoopery process is tested
The quality of effect controls.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of adult feedstuffs, in terms of mass percentage, crude protein content >=30%, and crude fat content
>=5%, crude fiber content≤6%, crude ash content≤12%, total phosphorus content >=1.0%；
The sum of content of above-mentioned each component is 100%.
In some specific embodiments of the invention, in terms of mass percentage, comprising: fish meal 20%~30%, greatly
Soya-bean cake 8%~18%, shelled peanut cake 5%~15%, wheat-middlings 10%~11%, wheat bran 10%~11%, barley 6%~8%,
Corn 10%~11%, rice bran 5%~8%, mealy potato 6%~9%, fish oil 1%, multidimensional pre-mixing agent 1%, astaxanthin 0.5%.
In some specific embodiments of the invention, in terms of mass percentage, comprising: fish meal 20%, soybean cake
15%, shelled peanut cake 15%, wheat-middlings 10%, wheat bran 10%, corn 10%, mealy potato 6.5%, barley 6%, rice bran 5%, fish
Oil 1%, multidimensional pre-mixing agent 1%, astaxanthin 0.5%.
In some specific embodiments of the invention, in terms of mass percentage, comprising: fish meal 25%, soybean cake
11%, shelled peanut cake 11%, wheat-middlings 11%, wheat bran 11%, corn 10%, mealy potato 7%, barley 6.5%, rice bran 5%, fish
Oil 1%, multidimensional pre-mixing agent 1%, astaxanthin 0.5%.
In some specific embodiments of the invention, in terms of mass percentage, comprising: fish meal 30%, soybean cake
10%, shelled peanut cake 6%, wheat-middlings 11%, wheat bran 11%, corn 11%, mealy potato 6.5%, barley 6%, rice bran 6%, fish
Oil 1%, multidimensional pre-mixing agent 1%, astaxanthin 0.5%.
In some specific embodiments of the invention, in terms of mass percentage, the multidimensional pre-mixing agent includes: dimension life
Plain B1 0.10%, vitamin B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate 0.50%, inositol
4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, vitamin C
1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E (50.00%)
2.00%, Vitamin K3 0.10%, flour 89.76%.
On the basis of the studies above, application of the adult feedstuff provided by the invention in Model fish cultivation.
In some specific embodiments of the invention, the fish are to test red crucian carp.
The present invention also provides a kind of cultural methods for testing red crucian carp adult fish, with the red crucian carp of adult feedstuff administering transgenic
Adult fish.
Suggest that day feeding rate is 1%~3% in some specific embodiments of the invention, in the cultural method.
The present invention provides a kind of completely new perfect compound feed for the red crucian carp adult fish of laboratory aquarium raising experiment,
Domestic market blank is filled up.With the red crucian carp adult feedstuff of experiment made of this feed formula, nutrition is balanced, and palatability is good, feed
Conversion ratio is also higher；Both it had been able to satisfy the nutritional need for testing red crucian carp normal growth and normally breed lived in aquarium,
Control requirement of the Laboratory Animal Standardization management process to feeding quality can be reached；Not only nontoxic, harmless but also not dirty to red crucian carp is tested
It contaminates aquarium aquatic environment (table 1).
Specific embodiment
The invention discloses a kind of red crucian carp adult feedstuffs of experiment and its application, those skilled in the art to use for reference in this paper
Hold, is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to those skilled in the art
For be it will be apparent that they are considered as being included in the present invention.Method and application of the invention, which has passed through, preferably to be implemented
Example is described, related personnel obviously can not depart from the content of present invention, in spirit and scope to method described herein and
Using being modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
The present invention is achieved through the following technical solutions: a kind of Major Nutrient index for testing red crucian carp adult feedstuff is true
Be set to: crude protein content >=30%, crude fat content >=5%, crude fiber content≤6%, crude ash content≤12%, total phosphorus contain
Amount >=1.0%；Raw material selection are as follows: fish meal, soybean cake, shelled peanut cake, wheat-middlings, wheat bran, corn, mealy potato, barley, rice bran,
11 kinds of fish oil, astaxanthin etc., separately add dimension pre-mixing agent.Feed formula is formed by the above raw material and its weight percent are as follows: fish meal
20%~30%, soybean cake 8%~18%, shelled peanut cake 5%~15%, wheat-middlings 10%~11%, wheat bran 10%~11%,
Barley 6%~8%, corn 10%~11%, rice bran 5%~8%, mealy potato 6%~9%, fish oil 1%, multidimensional pre-mixing agent
1%, astaxanthin 0.5%.
Further, multidimensional pre-mixing agent described in a kind of red crucian carp adult feedstuff formula of experiment then presses the following raw material and its weight
Percentage composition: vitamin B1 0.10%, vitamin B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate
0.50%, inositol 4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, dimension
Raw element C 1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E
(50.00%) 2.00%, Vitamin K3 0.10%, flour 89.76%.
Testing red crucian carp adult feedstuff has following optimization formula:
In some specific embodiments of the invention, formula are as follows: fish meal 20%, soybean cake 15%, shelled peanut cake 15%,
Wheat-middlings 10%, wheat bran 10%, corn 10%, mealy potato 6.5%, barley 6%, rice bran 5%, fish oil 1%, multidimensional pre-mixing agent
1%, astaxanthin 0.5%.
In some specific embodiments of the invention, formula are as follows: fish meal 25%, soybean cake 11%, shelled peanut cake 11%,
Wheat-middlings 11%, wheat bran 11%, corn 10%, mealy potato 7%, barley 6.5%, rice bran 5%, fish oil 1%, multidimensional pre-mixing agent
1%, astaxanthin 0.5%.
In some specific embodiments of the invention, formula are as follows: fish meal 30%, soybean cake 10%, shelled peanut cake 6%,
Wheat-middlings 11%, wheat bran 11%, corn 11%, mealy potato 6.5%, barley 6%, rice bran 6%, fish oil 1%, multidimensional pre-mixing agent
1%, astaxanthin 0.5%.
The present invention provides a kind of completely new adult fish perfect compound feed for the red crucian carp of laboratory aquarium raising experiment,
Domestic market blank is filled up.With the red crucian carp adult feedstuff of experiment made of this feed formula, nutrition is balanced, and palatability is good, feed
Conversion ratio is also higher；Both it had been able to satisfy the nutritional need for testing red crucian carp normal growth and normally breed lived in aquarium,
Control requirement of the Laboratory Animal Standardization management process to feeding quality can be reached；Not only nontoxic, harmless but also not dirty to red crucian carp is tested
It contaminates aquarium aquatic environment (table 1).
Raw materials used and auxiliary material is available on the market in the red crucian carp adult feedstuff of experiment provided by the invention and its application.
Below with reference to embodiment, the present invention is further explained:
Embodiment 1
Fish meal 20%, soybean cake 15%, shelled peanut cake 15%, wheat-middlings 10%, wheat bran 10%, corn 10%, mealy potato
6.5%, barley 6%, rice bran 5%, fish oil 1%, multidimensional pre-mixing agent 1%, astaxanthin 0.5%.Multidimensional premixes agent prescription are as follows: dimension life
Plain B1 0.10%, vitamin B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate 0.50%, inositol
4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, vitamin C
1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E (50.00%)
2.00%, Vitamin K3 0.10%, flour 89.76%.
Embodiment 2
Fish meal 25%, soybean cake 11%, shelled peanut cake 11%, wheat-middlings 11%, wheat bran 11%, corn 10%, mealy potato
7%, barley 6.5%, rice bran 5%, fish oil 1%, multidimensional pre-mixing agent 1%, astaxanthin 0.5%.Multidimensional premixes agent prescription are as follows: dimension life
Plain B1 0.10%, vitamin B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate 0.50%, inositol
4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, vitamin C
1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E (50.00%)
2.00%, Vitamin K3 0.10%, flour 89.76%.
Embodiment 3
Fish meal 30%, soybean cake 10%, shelled peanut cake 6%, wheat-middlings 11%, wheat bran 11%, corn 11%, mealy potato
6.5%, barley 6%, rice bran 6%, fish oil 1%, multidimensional pre-mixing agent 1%, astaxanthin 0.5%.Multidimensional premixes agent prescription are as follows: dimension life
Plain B1 0.10%, vitamin B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate 0.50%, inositol
4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, vitamin C
1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E (50.00%)
2.00%, Vitamin K3 0.10%, flour 89.76%.
4 culture experiment of embodiment
The red crucian carp adult fish of the experiment of 50mm or more a length of for body feeds feeding made of the formula announced by the invention patent
Material, partial size are 2.0~3.0mm, are fed daily 1~2 time, and day feeding rate is 1%~3%.Every 7~10d changes water l times, changes every time
1/3~1/2 water out, and remove dirt.
Experimental group: the red crucian carp adult fish of experiment at 10 monthly ages is fed with feed made from the embodiment of the present invention 1~3 respectively；
Control group: the red crucian carp adult fish of experiment at 10 monthly ages is fed with commercially available goldfish koi feed.
Test the culture efficiency such as table 1 of red crucian carp adult feedstuff.
Table 1 tests red crucian carp adult feedstuff culture efficiency comparison
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of adult feedstuff, which is characterized in that in terms of mass percentage, crude protein content >=30%, crude fat content >=
5%, crude fiber content≤6%, crude ash content≤12%, total phosphorus content >=1.0%；
The sum of content of above-mentioned each component is 100%.
2. adult feedstuff as described in claim 1, which is characterized in that in terms of mass percentage, comprising: fish meal 20%~
30%, soybean cake 8%~18%, shelled peanut cake 5%~15%, wheat-middlings 10%~11%, wheat bran 10%~11%, barley
6%~8%, corn 10%~11%, rice bran 5%~8%, mealy potato 6%~9%, fish oil 1%, multidimensional pre-mixing agent 1%, shrimp
Green element 0.5%.
3. adult feedstuff as claimed in claim 2, which is characterized in that in terms of mass percentage, comprising: fish meal 20%, greatly
Soya-bean cake 15%, shelled peanut cake 15%, wheat-middlings 10%, wheat bran 10%, corn 10%, mealy potato 6.5%, barley 6%, rice bran
5%, fish oil 1%, multidimensional pre-mixing agent 1%, astaxanthin 0.5%.
4. adult feedstuff as claimed in claim 2, which is characterized in that in terms of mass percentage, comprising: fish meal 25%, greatly
Soya-bean cake 11%, shelled peanut cake 11%, wheat-middlings 11%, wheat bran 11%, corn 10%, mealy potato 7%, barley 6.5%, rice bran
5%, fish oil 1%, multidimensional pre-mixing agent 1%, astaxanthin 0.5%.
5. adult feedstuff as claimed in claim 2, which is characterized in that in terms of mass percentage, comprising: fish meal 30%, greatly
Soya-bean cake 10%, shelled peanut cake 6%, wheat-middlings 11%, wheat bran 11%, corn 11%, mealy potato 6.5%, barley 6%, rice bran
6%, fish oil 1%, multidimensional pre-mixing agent 1%, astaxanthin 0.5%.
6. such as the described in any item adult feedstuffs of claim 2 to 5, which is characterized in that in terms of mass percentage, the multidimensional
Pre-mixing agent includes: vitamin B1 0.10%, vitamin B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate
0.50%, inositol 4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, dimension
Raw element C 1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E
(50.00%) 2.00%, Vitamin K3 0.10%, flour 89.76%.
7. such as application of the adult feedstuff as claimed in any one of claims 1 to 6 in Model fish cultivation.
8. the use as claimed in claim 7, which is characterized in that the fish are to test red crucian carp.
9. a kind of cultural method for testing red crucian carp adult fish, which is characterized in that with adult fish such as claimed in any one of claims 1 to 6
The red crucian carp adult fish of feed administering transgenic.
10. cultural method as claimed in claim 9, which is characterized in that day feeding rate is 1%~3%.
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| CN201910721225.9A CN110301559A (en) | 2019-08-06 | 2019-08-06 | Test adult feedstuff and its application of red crucian carp |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381706A (en) * | 2014-12-10 | 2015-03-04 | 青岛德润电池材料有限公司 | Aquarium goldfish feed and preparation method thereof |
-
2019
- 2019-08-06 CN CN201910721225.9A patent/CN110301559A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381706A (en) * | 2014-12-10 | 2015-03-04 | 青岛德润电池材料有限公司 | Aquarium goldfish feed and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 丁淑荃，等: "观赏鱼金鲫饲料配方的优化试验", 《安徽农业科学》 * |
| 刘娟，等: "实验红鲫水族箱饲养技术", 《实验动物科学》 * |
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Application publication date: 20191008 |