CN110269159A - Test feed for fingerling and its application of red crucian carp - Google Patents

Test feed for fingerling and its application of red crucian carp Download PDF

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Publication number
CN110269159A
CN110269159A CN201910721522.3A CN201910721522A CN110269159A CN 110269159 A CN110269159 A CN 110269159A CN 201910721522 A CN201910721522 A CN 201910721522A CN 110269159 A CN110269159 A CN 110269159A
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China
Prior art keywords
feed
vitamin
fingerling
red crucian
wheat
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CN201910721522.3A
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Chinese (zh)
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吴瑶瑶
吴端生
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Hengyang Normal University
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Hengyang Normal University
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Priority to CN201910721522.3A priority Critical patent/CN110269159A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Insects & Arthropods (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Husbandry (AREA)
  • Birds (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The present invention relates to fish culture fields, in particular to test scrod feed and its application in red crucian kind stage.The red crucian kind feed formula of the experiment, it is formed by the following raw material and its weight percent are as follows: fish meal 30%~35%, soybean 6%~12%, shelled peanut cake 5%~15%, wheat-middlings 9%~12%, wheat bran 8%~11%, corn 8%~11%, rice bran 5%~10%, mealy potato 5%~10%, brewer's yeast 0%~5%, fish oil 1%, multidimensional pre-mixing agent 1%.Its Major Nutrient index meets: crude protein content >=33%, crude fat content >=6%, crude fiber content≤6%, crude ash content≤12%, total phosphorus content >=1.2%.The feed formulation nutrients are balanced, good to the red crucian carp palatability of experiment, and feed conversion rate is higher, has both been able to satisfy the nutritional need for testing red crucian kind normal growth and development, are also beneficial to carry out effective quality control to the red crucian carp of experiment and its environment.

Description

Test feed for fingerling and its application of red crucian carp
Technical field
The present invention relates to fish culture fields, in particular to test fry feed and its application of red crucian carp.
Background technique
Testing red crucian carp is a kind of emerging native country Model fish in China, has important application value.Testing red crucian carp is water Raw poikilotherm tests red crucian carp suitable for water environment and tames by red crucian carp, is a kind of aquatic poikilotherm, is 0 DEG C in temperature Can survive in~39 DEG C of water body, and lower oxygen concentration resistance (O.lmg/L), in barrier environment the water temperature of aquarium can be controlled in 18 DEG C~ 24 DEG C, it is applicable to the zoopery of water environment, the advantage with Aquatic Ecotoxicology research and environmental safety evaluation. Testing red crucian carp has the morphological feature for being adapted to live in water ecological environment, and figure suitably tests red crucian carp in spindle figure, body Side is flat, full squama, mouth end position, without single tail is similar to carp.The dorsal fin fin for testing red crucian carp C1HD system (a kind of inbred strais) is D. I ~II-16~19, anal fin fin are A. I -5~7, and pectoral fin fin is P. I -14~17, and abdomeinal fin fin is V. I -7~8, lateral line scales Scale formula is.A length of 100~the 150mm of adult body.The figure for testing this size of red crucian carp is well suited for testing, it can also be anti- Living body is carried out again and takes blood, and disposable extraction blood volume will not be lethal up to 1.0~1.5mL.Such as it is suitble to do heavy metal, chemicals life Object enrichment experiment, convenient for taking the tissues such as liver, kidney to be analyzed.Testing red crucian carp has more perfect tract and physiological function, Similar mammal.Red crucian carp is tested with more perfect digestive system, the circulatory system, excretory system, nervous system, endocrine system System, reproductive system etc., the structure and function of tract are similar to mammal.What it is different from mammal is exhaled with the gill It inhales, heart is 1 atrium, 1 ventricle, and red blood cell has nucleus, the platelet-free with thrombocyte.Testing red crucian carp has in vitro Oviposition, ovum diameter big (680~720 μm), in vitro fertilization, ectogenetic development characteristics, are suitable for doing gene transfer, development biology It learns and reproductive biology is studied.The gill can be used as membrane physiology research model;M cell (Mauthner cell) can be used as research maincenter The model of nervous function;Mast cell (mast cell, MC) can be used for immunological investigation;Modified Retinal Model can be constructed to grind Study carefully optic nerve physiology;Reproducible liver tumor model, for carrying out oncology studies etc..
It is clear to test red crucian carp genetic background, is standardized Fish as Laboratory Animal.Test red crucian carp chromosome number 2n= 100, karyotype formulas 22m+30sm+24st+24t, full-length genome size are 2.16Gb, and number of genes is 44580.Relative to The red blood cell DNA content of chicken is 2.5pg, and the red blood cell DNA content of red crucian carp is 3.0pg, minimum in cultivation Strains of Carassius auratus, is shown Red crucian carp has the advantages that model organism.Testing red crucian carp C1HD system is a kind of pure lines derived from gynogenesis, principal biological number Mark (such as RAPD, SSR, AFLP, SNP are marked) it has been established that suitable for carrying out genome walking, turning according to central genetic The research such as gene studies, genetic analysis and sexual control.
The oviposition for testing red crucian carp is more, and reproductive capacity is strong, in liberal supply.1~2 age tested red crucian carp, and just sexal maturity, fish brood amount reach 1.5~80,000, primary oviposition also up to 30,000 can several times a year lay eggs, therefore reproductive capacity is strong, ample supply of commodities on the market.
The body colour for testing red crucian carp is entirely red, and watches and be integrated with experiment.The mutation that red crucian carp is crucian carp is tested, and goldfish close relative, Its body colour is complete red gorgeous, is both the excellent instruction of a kind of fresh water fancy fishes and Drinking Water and water resource pollution monitoring Biology, it may also be used for genetics research, such as the research of color inheritance, tail fin character variation.Its viability is strong, is easy to Shui nationality cage breeding Growing the red crucian carp of experiment is omnivorousness, and viability is stronger, and raising is easy in aquarium, can also be developed up to sexal maturity.It feeds and manually matches Feed is closed, similar breeding goldfish is equally raised, handy.It is suitable for aquarium cultivation and experimental study.
It is domestic at present to appear on the market not yet dedicated for the red crucian carp perfect compound feed commodity of raising experiment.The reality cultivated indoors It tests in red crucian carp aquaculture, in the scientific experimentation that the red crucian carp of application experiment is carried out, lacks the standardized red crucian carp full price of experiment of one kind and match Close feed, seriously affect the red crucian carp of experiment normally produce it is accurate with scientific experimentation result.Therefore, special in testing red crucian carp aquaculture It is not to provide a kind of standardization during carrying out scientific research and environmental safety evaluation as experimental material to test red crucian carp The feed for fingerling of the red crucian carp of experiment have important practical significance.
Summary of the invention
In view of this, the present invention provides a kind of fry feed for testing red crucian carp and its application.The feed formulation nutrients are balanced, Good to the red crucian carp palatability of experiment, feed conversion rate is higher, has both been able to satisfy the nutritional need for testing red crucian kind normal growth and development, It is also beneficial to carry out effective quality control to the red crucian carp of experiment and its environment.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides feed for fingerling, in terms of mass percentage, crude protein content >=33%, crude fat content >= 6%, crude fiber content≤6%, crude ash content≤12%, total phosphorus content >=1.2%;
The sum of content of above-mentioned each component is 100%.
In some specific embodiments of the invention, in terms of mass percentage, comprising: fish meal 30%~35%, greatly Beans 6%~12%, shelled peanut cake 5%~15%, wheat-middlings 9%~12%, wheat bran 8%~11%, corn 8%~11%, rice Chaff 5%~10%, mealy potato 5%~10%, brewer's yeast 0%~5%, fish oil 1%, multidimensional pre-mixing agent 1%.
In some specific embodiments of the invention, in terms of mass percentage, comprising: fish meal 30%, soybean 10%, Shelled peanut cake 10%, wheat-middlings 10%, wheat bran 10%, corn 9%, mealy potato 9%, rice bran 5%, brewer's yeast 5%, fish oil 1%, multidimensional pre-mixing agent 1%.
In some specific embodiments of the invention, in terms of mass percentage, comprising: fish meal 33%, soybean 8%, Shelled peanut cake 8%, wheat-middlings 10%, wheat bran 10%, corn 10%, mealy potato 9%, rice bran 5%, brewer's yeast 5%, fish oil 1%, multidimensional pre-mixing agent 1%.
In some specific embodiments of the invention, in terms of mass percentage, comprising: fish meal 35%, soybean 8%, Shelled peanut cake 5%, wheat-middlings 10%, wheat bran 10%, corn 10%, mealy potato 10%, rice bran 5%, brewer's yeast 5%, fish oil 1%, multidimensional pre-mixing agent 1%.
In some specific embodiments of the invention, in terms of mass percentage, the multidimensional pre-mixing agent includes: dimension life Plain B1 0.10%, vitamin B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate 0.50%, inositol 4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, vitamin C 1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E (50.00%) 2.00%, Vitamin K3 0.10%, flour 89.76%.
On the basis of the studies above, the present invention also provides application of the feed for fingerling in Model fish cultivation.
In some specific embodiments of the invention, the fish are to test red crucian carp.
The present invention also provides a kind of cultural methods for testing red carp fry, with the red crucian carp of fry feed administering transgenic Fingerling.
In some specific embodiments of the invention, the dosage of the feeding is that day feeding rate is 3%~5%.
The present invention provides feed for fingerling, in terms of mass percentage, crude protein content >=33%, crude fat content >= 6%, crude fiber content≤6%, crude ash content≤12%, total phosphorus content >=1.2%;The sum of the content of above-mentioned each component is 100%.A kind of completely new red crucian kind perfect compound feed of experiment cultivated for laboratory aquarium provided by the invention, is filled out Domestic market blank is mended.With the red crucian carp feed for fingerling of experiment made of this feed formula, nutrition is balanced, and palatability is good, and feed turns Rate is also higher;It is able to satisfy the nutritional need for testing red crucian kind normal growth and development, nothing nontoxic to the red crucian carp growth and development of experiment Evil can not pollute aquarium aquatic environment (table 1) to red crucian carp and its effective quality control of environment progress is tested yet.
Specific embodiment
The invention discloses a kind of feed for fingerling for testing red crucian carp and its application, those skilled in the art can be used for reference herein Content is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to those skilled in the art It is it will be apparent that they are considered as being included in the present invention for member.Method and application of the invention has passed through preferably real Example is applied to be described, related personnel obviously can not depart from the content of present invention, in spirit and scope to method described herein It is modified or appropriate changes and combinations with application, carrys out implementation and application the technology of the present invention.
The present invention is achieved through the following technical solutions: a kind of Major Nutrient index for testing red crucian carp feed for fingerling is true Be set to: crude protein content >=33%, crude fat content >=6%, crude fiber content≤6%, crude ash content≤12%, total phosphorus contain Amount >=1.2;This feed formula is formed by following raw material and its weight percent are as follows: fish meal 30%~35%, soybean 6%~ 12%, shelled peanut cake 5%~15%, wheat-middlings 9%~12%, wheat bran 8%~11%, corn 8%~11%, rice bran 5%~ 10%, mealy potato 5%~10%, brewer's yeast 0%~5%, fish oil 1%, multidimensional pre-mixing agent 1%.
Further, multidimensional pre-mixing agent described in a kind of red crucian kind feed formula of experiment then presses the following raw material and its weight Percentage composition: vitamin B1 0.10%, vitamin B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate 0.50%, inositol 4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, dimension Raw element C 1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E (50.00%) 2.00%, Vitamin K3 0.10%, flour 89.76%.
Testing red crucian carp feed for fingerling has following optimization formula:
Optimization formula 1: fish meal 30%, soybean 10%, shelled peanut cake 10%, wheat-middlings 10%, wheat bran 10%, corn 9%, Mealy potato 9%, rice bran 5%, brewer's yeast 5%, fish oil 1%, multidimensional pre-mixing agent 1%.
Optimization formula 2: fish meal 33%, soybean 8%, shelled peanut cake 8%, wheat-middlings 10%, wheat bran 10%, corn 10%, Mealy potato 9%, rice bran 5%, brewer's yeast 5%, fish oil 1%, multidimensional pre-mixing agent 1%.
Optimization formula 3: fish meal 35%, soybean 8%, shelled peanut cake 5%, wheat-middlings 10%, wheat bran 10%, corn 10%, Mealy potato 10%, rice bran 5%, brewer's yeast 5%, fish oil 1%, multidimensional pre-mixing agent 1%.
The present invention provides a kind of completely new red crucian kind perfect compound feed of experiment cultivated for laboratory aquarium, Domestic market blank is filled up.With the red crucian carp feed for fingerling of experiment made of this feed formula, nutrition is balanced, and palatability is good, feed Conversion ratio is also higher;It is able to satisfy the nutritional need for testing red crucian kind normal growth and development, nontoxic to the red crucian carp growth and development of experiment, It is harmless, aquarium aquatic environment can not be polluted to red crucian carp and its effective quality control of environment progress is tested yet.
Raw materials used and auxiliary material is available on the market in the fry feed of the red crucian carp of experiment provided by the invention and its application.
Below with reference to embodiment, the present invention is further explained:
Embodiment 1
Fish meal 30%, soybean 10%, shelled peanut cake 10%, wheat-middlings 10%, wheat bran 10%, corn 9%, mealy potato 9%, Rice bran 5%, brewer's yeast 5%, fish oil 1%, multidimensional pre-mixing agent 1%.Multidimensional premixes agent prescription are as follows: vitamin B1 0.10%, dimension Raw element B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate 0.50%, inositol 4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, vitamin C 1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E (50.00%) 2.00%, Vitamin K3 0.10%, flour 89.76%.
Embodiment 2
Fish meal 33%, soybean 8%, shelled peanut cake 8%, wheat-middlings 10%, wheat bran 10%, corn 10%, mealy potato 9%, Rice bran 5%, brewer's yeast 5%, fish oil 1%, multidimensional pre-mixing agent 1%.Multidimensional premixes agent prescription are as follows: vitamin B1 0.10%, dimension Raw element B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate 0.50%, inositol 4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, vitamin C 1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E (50.00%) 2.00%, Vitamin K3 0.10%, flour 89.76%.
Embodiment 3
Fish meal 35%, soybean 8%, shelled peanut cake 5%, wheat-middlings 10%, wheat bran 10%, corn 10%, mealy potato 10%, Rice bran 5%, brewer's yeast 5%, fish oil 1%, multidimensional pre-mixing agent 1%.Multidimensional premixes agent prescription are as follows: vitamin B1 0.10%, dimension Raw element B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate 0.50%, inositol 4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, vitamin C 1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E (50.00%) 2.00%, Vitamin K3 0.10%, flour 89.76%.
Embodiment 4
For example, the red crucian kind of the experiment of 20mm~50mm a length of for body, feeds this patent and is formulated manufactured particulate material, grain Diameter is 0.5~1.5mm, is fed daily 1~2 time, and day feeding rate is 3%~5%.
Experimental group: respectively with the fingerling of the red crucian carp of feed administering transgenic made from the embodiment of the present invention 1~3;
Control group: with the fingerling of the commercially available red crucian carp of goldfish koi feed administering transgenic.
Test the culture efficiency such as table 1 of red crucian carp feed for fingerling.
Table 1 tests the culture efficiency comparison of red crucian carp feed for fingerling
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims (10)

1. feed for fingerling, which is characterized in that in terms of mass percentage, crude protein content >=33%, crude fat content >=6%, Crude fiber content≤6%, crude ash content≤12%, total phosphorus content >=1.2%;
The sum of content of above-mentioned each component is 100%.
2. feed for fingerling as described in claim 1, which is characterized in that in terms of mass percentage, comprising: fish meal 30%~ 35%, soybean 6%~12%, shelled peanut cake 5%~15%, wheat-middlings 9%~12%, wheat bran 8%~11%, corn 8%~ 11%, rice bran 5%~10%, mealy potato 5%~10%, brewer's yeast 0%~5%, fish oil 1%, multidimensional pre-mixing agent 1%.
3. feed for fingerling as described in claim 1, which is characterized in that in terms of mass percentage, comprising: fish meal 30%, greatly Beans 10%, shelled peanut cake 10%, wheat-middlings 10%, wheat bran 10%, corn 9%, mealy potato 9%, rice bran 5%, brewer's yeast 5%, fish oil 1%, multidimensional pre-mixing agent 1%.
4. feed for fingerling as described in claim 1, which is characterized in that in terms of mass percentage, comprising: fish meal 33%, greatly Beans 8%, shelled peanut cake 8%, wheat-middlings 10%, wheat bran 10%, corn 10%, mealy potato 9%, rice bran 5%, brewer's yeast 5%, Fish oil 1%, multidimensional pre-mixing agent 1%.
5. feed for fingerling as described in claim 1, which is characterized in that in terms of mass percentage, comprising: fish meal 35%, greatly Beans 8%, shelled peanut cake 5%, wheat-middlings 10%, wheat bran 10%, corn 10%, mealy potato 10%, rice bran 5%, brewer's yeast 5%, fish oil 1%, multidimensional pre-mixing agent 1%.
6. such as feed for fingerling described in any one of claim 1 to 5, which is characterized in that in terms of mass percentage, the multidimensional Pre-mixing agent includes: vitamin B1 0.10%, vitamin B2 0.30%, vitamin B6 0.09%, niacin 1.00%, calcium pantothenate 0.50%, inositol 4.00%, biotin (2.00%) 0.08%, folic acid 0.02%, vitamin B12 (0.10%) 0.15%, dimension Raw element C 1.50%, vitamin A (500000IU/g) 0.20%, vitamine D3 (100000IU/g) 0.20%, vitamin E (50.00%) 2.00%, Vitamin K3 0.10%, flour 89.76%.
7. such as application of the feed for fingerling as claimed in any one of claims 1 to 6 in Model fish cultivation.
8. the use as claimed in claim 7, which is characterized in that the fish are to test red crucian carp.
9. a kind of cultural method for testing red carp fry, which is characterized in that with fry such as claimed in any one of claims 1 to 6 The fingerling of the red crucian carp of feed administering transgenic.
10. cultural method as claimed in claim 9, which is characterized in that feeding rate is 1%~3%.
CN201910721522.3A 2019-08-06 2019-08-06 Test feed for fingerling and its application of red crucian carp Pending CN110269159A (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1751588A (en) * 2005-10-21 2006-03-29 上海水产大学 Health-care type fish-bait for crucian

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1751588A (en) * 2005-10-21 2006-03-29 上海水产大学 Health-care type fish-bait for crucian

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
刘娟等: "实验红鲫水族箱饲养技术", 《实验动物科学》 *
王冬武 等: "《淡水鱼标准化养殖操作手册》", 31 October 2017, 湖南科学技术出版社 *
石文雷 等: "《鱼虾蟹高效益饲料配方 第2版》", 31 January 2007 *

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