CN110267643A - The composition for beautifying skin including the albumen that flocculates - Google Patents
The composition for beautifying skin including the albumen that flocculates Download PDFInfo
- Publication number
- CN110267643A CN110267643A CN201980000340.5A CN201980000340A CN110267643A CN 110267643 A CN110267643 A CN 110267643A CN 201980000340 A CN201980000340 A CN 201980000340A CN 110267643 A CN110267643 A CN 110267643A
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- Prior art keywords
- skin
- composition
- albumen
- yeast
- saccharomyces
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- 239000003792 electrolyte Substances 0.000 description 1
- 239000003974 emollient agent Substances 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
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- 239000000796 flavoring agent Substances 0.000 description 1
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- 235000013355 food flavoring agent Nutrition 0.000 description 1
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- 238000009472 formulation Methods 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- LBQIJVLKGVZRIW-ZDUSSCGKSA-N glabridin Chemical compound C1([C@H]2CC3=CC=C4OC(C=CC4=C3OC2)(C)C)=CC=C(O)C=C1O LBQIJVLKGVZRIW-ZDUSSCGKSA-N 0.000 description 1
- 229940093767 glabridin Drugs 0.000 description 1
- PMPYOYXFIHXBJI-ZDUSSCGKSA-N glabridin Natural products C1([C@H]2CC=3C=CC4=C(C=3OC2)CCC(O4)(C)C)=CC=C(O)C=C1O PMPYOYXFIHXBJI-ZDUSSCGKSA-N 0.000 description 1
- LBQIJVLKGVZRIW-UHFFFAOYSA-N glabridine Natural products C1OC2=C3C=CC(C)(C)OC3=CC=C2CC1C1=CC=C(O)C=C1O LBQIJVLKGVZRIW-UHFFFAOYSA-N 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 229960001031 glucose Drugs 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 description 1
- 229960004949 glycyrrhizic acid Drugs 0.000 description 1
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 description 1
- 239000001685 glycyrrhizic acid Substances 0.000 description 1
- 235000019410 glycyrrhizin Nutrition 0.000 description 1
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000001023 inorganic pigment Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 1
- 229960004705 kojic acid Drugs 0.000 description 1
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 1
- 210000002414 leg Anatomy 0.000 description 1
- 229940010454 licorice Drugs 0.000 description 1
- 229940078752 magnesium ascorbyl phosphate Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000012860 organic pigment Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- LCLHHZYHLXDRQG-ZNKJPWOQSA-N pectic acid Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)O[C@H](C(O)=O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](OC2[C@@H]([C@@H](O)[C@@H](O)[C@H](O2)C(O)=O)O)[C@@H](C(O)=O)O1 LCLHHZYHLXDRQG-ZNKJPWOQSA-N 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 235000013550 pizza Nutrition 0.000 description 1
- 239000010318 polygalacturonic acid Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 229960002181 saccharomyces boulardii Drugs 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 210000004761 scalp Anatomy 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000036560 skin regeneration Effects 0.000 description 1
- 235000011888 snacks Nutrition 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000015424 sodium Nutrition 0.000 description 1
- 235000019983 sodium metaphosphate Nutrition 0.000 description 1
- 235000019830 sodium polyphosphate Nutrition 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 229940042585 tocopherol acetate Drugs 0.000 description 1
- 229960000401 tranexamic acid Drugs 0.000 description 1
- GYDJEQRTZSCIOI-LJGSYFOKSA-N tranexamic acid Chemical compound NC[C@H]1CC[C@H](C(O)=O)CC1 GYDJEQRTZSCIOI-LJGSYFOKSA-N 0.000 description 1
- HTJNEBVCZXHBNJ-XCTPRCOBSA-H trimagnesium;(2r)-2-[(1s)-1,2-dihydroxyethyl]-3,4-dihydroxy-2h-furan-5-one;diphosphate Chemical compound [Mg+2].[Mg+2].[Mg+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.OC[C@H](O)[C@H]1OC(=O)C(O)=C1O HTJNEBVCZXHBNJ-XCTPRCOBSA-H 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229960001722 verapamil Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
- WHNFPRLDDSXQCL-UAZQEYIDSA-N α-msh Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(N)=O)NC(=O)[C@H](CO)NC(C)=O)C1=CC=C(O)C=C1 WHNFPRLDDSXQCL-UAZQEYIDSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/177—Receptors; Cell surface antigens; Cell surface determinants
- A61K38/178—Lectin superfamily, e.g. selectins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Birds (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medical Informatics (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Polymers & Plastics (AREA)
- Immunology (AREA)
- Alternative & Traditional Medicine (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Cosmetics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
One aspect of the present invention provides a kind of composition for beautifying skin including the albumen that flocculates.Due to including that the composition of flocculation albumen has improvement skin barrier, moisturizing, whitening and cell Proliferation and other effects, and promotes collagen synthesis and elastin laminin to synthesize, therefore be effectively used for beautifying skin.
Description
Technical field
The present invention relates to a kind of compositions for beautifying skin.
Background technique
Flocculation albumen (Flocculin) is Pleurotus Ostreatus in glug brewer's yeast (Lager yeast) cell wall like egg
White (lectin like protein), for precipitating glug brewer's yeast.In particular, flocculation albumen is with excellent with mannose
Binding force, in conjunction with the mannose of other yeast walls and generate the sediment of yeast cells, and also there is protection yeast to exempt from
The function of the stress factors such as nutrient exhaustion, temperature and ultraviolet light (UV) during being cultivated.
On the other hand, yeast is as cosmetic composition, for improving the mesh of skin (such as improvement wrinkle and whitening effect)
's.After using yeast cells to make cell wall rupture by proteolytic enzyme, organic solvent or self-dissolving as raw material, remove insoluble
Part is simultaneously concentrated, Lai Shengcheng yeast extract, and the yeast extract is used as cosmetic composition.Yeast processed in this way
Extract is made of the compound of various protein, peptide, amino acid, minerals and B family vitamin, and is used as cosmetics and is added
Object.In addition, U.S. Patent No. 5,776, No. 441 and U.S. Patent No. 6,177,105 disclose yeast extract with skin
Wound healing and bone regeneration and collagen synthesis effect, and yeast extract is used as various cosmetic materials.But up to now, do not have
The albumen that flocculates is used as to the example of the purpose of beautifying skin in cosmetic composition.
Summary of the invention
Technical problem
One aspect of the present invention provides a kind of cosmetic composition for beautifying skin including the albumen that flocculates.
Another aspect of the present invention provides a kind of health functional food combination for beautifying skin including flocculation albumen
Object.
Another aspect of the present invention provides a kind of pharmaceutical composition for beautifying skin including the albumen that flocculates.
Another aspect of the present invention provides a kind of method for the treatment of or improvement skin injury or skin aging comprising: to a
Body applies a effective amount of composition for including the steps that flocculation albumen.
Another aspect of the present invention provides a kind of purposes of composition including the albumen that flocculates, and is used to prepare skin injury or skin
The therapeutic agent of skin aging.
Technical solution
One aspect of the present invention provides a kind of cosmetic composition for beautifying skin including the albumen that flocculates.The present invention is another
On the one hand a kind of health functional food composition for beautifying skin including the albumen that flocculates is provided.Another aspect of the present invention
A kind of pharmaceutical composition for being used to prevent or treat skin injury or skin aging including flocculation albumen is provided.The present invention is another
Aspect provides a kind of Dermatologic preparation composition for beautifying skin.
" flocculation albumen " typically refers to the Lectin-like protein in the cell wall expressed in Blastocystis microorganism
(lectin like protein), and (Marika) H. (1994. flocculation albumen from brewer's yeast can be blocked according to Mali
Purifying and Partial Feature (Purification and partial characterization of a flocculin
From brewer's yeast)) etc. method disclosed in documents commercial purchase is separated or passed through from Blastocystis microorganism
Buy acquisition.It is known that the Blastocystis microorganism include saccharomyces bayanus (Saccharomyces bayanus),
Bradley yeast (Saccharomyces boulardii), Bo Yiding yeast (Saccharomyces bulderi), Rio yeast
(Saccharomyces cariocanus), Mika Kallio library this yeast (Saccharomyces cariocus), saccharomyces cerevisiae
(Saccharomyces cerevisiae), Xue watt yeast (Saccharomyces chevalieri), Dalian sugar yeast
(Saccharomyces dairenensis), wine yeast (Saccharomyces ellipsoideus), true saccharomyces bayanus
(Saccharomyces eubayanus), saccharomyces exiguus (Saccharomyces exiguus), Fornari saccharomyces diastaticus
(Saccharomyces florentinus), Crewe take yeast (Saccharomyces kluyveri), martini sugar yeast
(Saccharomyces martiniae), it rubs and receives yeast (Saccharomyces monacensis), promise ground yeast
(Saccharomyces norbensis), Saccharomyces paradoxus (Saccharomyces paradoxus), saccharomyces pastorianus
(Saccharomyces pastorianus), Si Pansilong yeast (Saccharomyces spencerorum), Tu Lieci ferment
Mother (Saccharomyces turicensis), saccharomyces unisporus (Saccharomyces unisporus), saccharomyces uvarum
(Saccharomyces uvarum) and Zuo Natu yeast (Saccharomyces zonatus).Known flocculation albumen
Amino acid sequence includes, for example, NCBI registration number: AIC64115.1, AFJ20718.1, BAG49462.1 or ABS87371.1
Deng.
The composition can further include the culture or extract of the Blastocystis microorganism.Art technology
Personnel can be readily available the culture or extract according to methods known in the art.For example, the culture of microorganism can
To use commercially available culture medium to carry out, for example, LB culture medium, Ham's F10 culture (Sigma), minimum essential medium (MEM,
Sigma), RPMI-1640 culture medium (Sigma) and Dulbecco modified Eagle medium (DMEM, Sigma) etc..If any
It needs, hormone or other growth factors, salt, buffer, nucleotide, antibiotic, microelement and glucose etc. can be with known
Suitable concentration supplement.Condition of culture such as temperature can be suitably determined according to selected microorganism by those skilled in the art
Degree and pH etc..Furthermore it is possible to which or mixtures thereof water, acetone, alcohol (for example, C1-C6 alcohol) are used as solvent to extract the extraction
Object.The C1-C6 alcohol can be methanol, ethyl alcohol, propyl alcohol, isopropanol, 1,3- propylene glycol, butanol, amylalcohol or hexane.The solvent
It can be, for example, the mixture of water and alcohol, i.e. alcohol solution.The determining alcohol of alcohol solution can be 1 (v/v) % to 100 (v/
V) %, for example, 1 (v/v) % to 99.5 (v/v) %, 10 (v/v) % to 100 (v/v) %, 20 (v/v) % to 100 (v/v) %,
30 (v/v) % to 100 (v/v) %, 40 (v/v) % to 100 (v/v) %, 50 (v/v) % to 100 (v/v) %, 60 (v/v) % are extremely
100 (v/v) %, 70 (v/v) % to 100 (v/v) %, 75 (v/v) % to 100 (v/v) %, 60 (v/v) % to 90 (v/v) %,
60 (v/v) % to 80 (v/v) %, 65 (v/v) % of (v/v) % to 75 or 70 (v/v) %.The alcohol solution can be methanol-water
Solution, ethanol water or butanol aqueous solution.The composition can further comprise the fraction of the extract.Term " fraction
(fraction) " refer to the substance that extract is divided into the substance of part thereof of ingredient, that is, has been fractionated.The fraction can lead to
Cross solvent fractionation (fractionation) acquisition.The solvent fractionation, which can refer to mix extract with solvent and separate, deposits
The step of being the substance in the solvent.
Term " beautifying skin ", which refers to, improves the skin impaired due to skin injury or skin aging with wrinkle of skin,
The methods of enhancing richness, elasticity improvement or skin regeneration are restored to the state before damage.The skin includes that body is all
The skin at position, including face, hand, arm, leg, foot, chest, abdomen, back, buttocks and scalp.
Term " skin injury " includes the clinic of skin histology or cell and the damage of Treatment, including external physical damage
The infiltration of wound, chemical substance, bacterium, fungi and virus etc., be exposed to ultraviolet light, skin water loss and aging etc. caused by wrinkle
Line, the oxidation as caused by free radical (active oxygen) etc., cutaneous pigmentation, scytitis, seborrheic skin are scorching, rubescent (red
Swollen, erythema), edema, mossization, eczema, scratchiness (itching) and atopical dermatitis etc..
Term " skin aging " includes intrinsic aging at any time and because extrinsic aging caused by external environment.It is described
Skin aging may include wrinkle of skin, flaw and foxiness.The wrinkle of skin can be due to thin caused by skin decline
Line.The reason of generating wrinkle of skin can be light aging, age, facial expression, shortage moisture or combinations thereof.The smooth aging can
To make skin aging and being exposed to ultraviolet light (including UVA, UVB and UVC).Wrinkle of skin improvement can be inhibition or
The generation of wrinkle of skin is hindered either to mitigate generated wrinkle.
The preparation type of the composition can be non-oral form of administration.Non-oral form of administration can be injection or
Skin preparations for extenal use.The skin preparations for extenal use can be emulsifiable paste, gel, ointment, skin emulsifier, skin suspension, percutaneous delivery
Property patch, contain sparadrap, lotion or combinations thereof.The skin preparations for extenal use, which can according to need to properly mix, is typically used as cosmetics
Or the ingredient of the skin preparations for extenal use of drug etc., for example, water composition, oil components, powdered ingredients, alcohols, moisturizer, thickening
Agent, ultraviolet absorbing agent, whitening agent, preservative, antioxidant, surfactant, fragrance, colorant, various skin-nourishing agent
Or combinations thereof.The skin preparations for extenal use can properly mix disodium ethylene diamine tetraacetate, sodium versenate, citric acid
The metal-chelator of sodium, sodium polyphosphate, sodium metaphosphate, gluconic acid etc., caffeine, tannin, verapamil, licorice,
Glabridin, the hot water extract of setose thistle fruit, various crude drugs, tocopherol acetate, glycyrrhizic acid, tranexamic acid and its derivative
Or the medicament of its salt etc., vitamin C, magnesium ascorbyl phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fruit
The carbohydrate of sugar, trehalose etc..
When the composition is cosmetic composition, cosmetic composition can be prepared as including dosage form below: makeup
Water (skin lotion), smoothing toner, toner, astringent (Astringent), lotion, milk lotion (milk lotion), moisturizing
It is lotion, nutrient solution, massage cream, nourishing cream, moisturiser, hand frost, foundation cream, essence, nutrition essence, film, soap, face cleaning foam, clean
Face cream, washing cream, body lotion, clean body liquid, suspension, gel, powder, viscous cream (paste), air cushion, facial mask or on chip
Facial mask or spray composite.The composition of such dosage form can be prepared according to conventional method in that art.The cosmetic composition can
With further comprise preservative agent, stabilizer, surfactant, thickener, solvent, moisturizer, emollient, ultraviolet absorbing agent,
Preservative, fungicide, emulsifier, antioxidant, pH adjusting agent, organic and inorganic pigment, fragrance, cold feeling agent or antiperspirant etc. exist
Additive commonly used in the art.In the range of not damaging the objects and effects of the present invention, those skilled in the art can be easy
Ground selects the combined amount of the supplementary element of above-mentioned preservative agent etc., which can be 0.001 of the total weight based on composition
Weight percent is to 40 weight percent.
When composition is pharmaceutical composition, described pharmaceutical composition may include a effective amount of flocculation albumen or including wadding
Coagulated protein is as effective component.The effective quantity can suitably be selected according to individual.The effective quantity can according to include with
The factor of lower item and in the field of medicine known to other factors determine: the severity of disease, the age of patient, body
Weight, health, gender, patient to the susceptibility of drug, administration time, administration method and discharge ratio, during treatment, with described group
The drug for closing object mixing or using simultaneously.Application is can be executed by any method known in the art.Application can pass through
Such as intravenous, intramuscular, oral, percutaneous (transdermal), mucous membrane, intranasal (intranasal), intratracheal or subcutaneously apply
Approach is directly executed to individual in any way.The application can be executed with whole body or part.
The individual can be mammal, such as: people, ox, horse, pig, dog, sheep, goat or cat.The individual can be with
It is the individual for needing to be improved beautifying skin (such as skin moisture-keeping, enhancing skin barrier or skin resurfacing effect).
The application, which can be, daily applies the composition for being used for beautifying skin including flocculation albumen according to following amount
To each individual: 0.1mg to 1000mg, for example, 0.1mg to 500mg, 0.1mg to 100mg, 0.1mg to 50mg, 0.1mg extremely
25mg, 1mg to 1,000mg, 1mg to 500mg, 1mg to 100mg, 1mg to 50mg, 1mg to 25mg, 5mg to 1000mg, 5mg extremely
500mg, 5mg to 100mg, 5mg to 50mg, 5mg to 25mg, 10mg to 1,000mg, 10mg to 500mg, 10mg to 100mg,
10mg to 50mg or 10mg to 25mg.However, amount of application can be according to such as formulation method, method of administration, the age of patient, body
Weight, gender, pathological condition, diet, administration time, administration method, drainage rate and draw property etc. because usually differently
Prescription, it will be understood by those skilled in the art that it is contemplated that these factors are with appropriate adjustment amount of application.Application times can be daily
1 time or clinically 2 times or more in acceptable side-effect profile, about site of administration, can be at one or at two more than
Application, and total application number of days that is daily or every 2 days to 5 days being interval can be and apply 1 day to 30 days in seance.Such as
It is in need, identical treatment can be repeated after a suitable time.It, can be with every kg amount of application and people for the animal in addition to people
Identical amount of application changes to apply, or with the volume ratio (for example, average value) etc. of target animal and the organ (heart etc.) of people
The amount of the amount of application is calculated to apply.
When the composition is health functional food composition, can the preparation type can be it is known in the art
Routine healthcare food preparation.The health food can be prepared as such as powder, granule, tablet, pill, capsule, hang
The general dosage form of supernatant liquid, finish, syrup, impregnating agent, liquor, extractant etc., and it is prepared as meat, sausage, bread, chalk
Power, confectionery, snack, Biscuits, pizza, hand-pulled noodles, other noodles, chewing gum class, jelly, the cream system including ice cream
The arbitrary health food form of product, various soup, beverage, tea, potus, alcoholic beverage and vitamin complex etc..In order to by institute
It states health food and preparation is made, acceptable carrier or additive in bromatology can be used, and known in the art any
Carrier or additive, which can be used for preparing, thinks preparation to be prepared.As the additive, various nutritional agents, dimension life can be contained
Element, electrolyte, flavoring agent, colorant, pectic acid and its salt, alginic acid and its salt, organic acid, protective colloid thickener, pH tune
Save agent, stabilizer, preservative, glycerol, alcohol, for carbonating agent of soda etc..In addition, the additive can contain it is useful
In the pulp for preparing fruit juice, fruit drink and vegetable beverage.These additive components can be used alone or be applied in combination,
It, can be for every 100 part composition by weight in 0.01 parts by weight to 0.1 parts by weight although the ratio of additive is not key
Selection in range.The content of the extract of the flocculation albumen in the health functional food composition can be according to using
Purpose (prevention or improve) suitably determines.In general, may include 0.01 weight percent to 15 weight of food total weight
Percentage, can be containing the ratio of 0.02g to 10g, more preferably 0.3g extremely on the basis of 100mL when as drink manufacturing
The ratio of 1g.
The present inventor in the test using the composition for including flocculation albumen with general yeast culture or not
Processing group is compared, it is thus identified that the significantly improving of the expression of silk polyprotein, the improvement of the expression of tight junction protein (claudin) 1,
The improvement of the expression of hyaluronic acid synthetase, the improvement of the expression of exfoliating enzyme and the effect for inhibiting melanin production.Therefore,
Composition including the albumen that flocculates is effectively used for beautifying skin or for treating skin related disease etc..
Beneficial effect
Improve skin barrier, moisturizing, whitening and cell Proliferation and other effects since the composition including flocculation albumen has, and
Promote collagen synthesis and elastin laminin synthesis, therefore is effectively used for beautifying skin.
Detailed description of the invention
Fig. 1 is shown in the increase effect for immortalizing the silk polyprotein expression of the albumen of the flocculation in epidermis (HaCat) cell
Chart.
Fig. 2 is shown in the increase effect for the expression of tight junction protein 1 for immortalizing the albumen of the flocculation in epidermal cell
Chart.
Fig. 3 is shown in the increasing for immortalizing hyaluronic acid synthase 3 (HAS3) expression of the albumen of the flocculation in epidermal cell
Add the chart of effect.
Fig. 4 is shown in the increase effect for immortalizing kallikrein 7 (KLK7) expression of the albumen of the flocculation in epidermal cell
The chart of fruit.
Fig. 5 is shown in the chart for immortalizing the effect of inhibition melanin production of the albumen of the flocculation in epidermal cell.
Specific embodiment
It is described in detail using embodiment and test example as example, specifically to illustrate the present invention.However, of the invention
Embodiment and test example can be changed in the form of a variety of different, and should not be interpreted as being limited to the scope of the present invention below
Described in embodiment and test example.These embodiments are provided and test example is to keep those skilled in the art more thorough
Bottom and it is fully understood by the present invention.
Embodiment 1: the preparation for the albumen that flocculates
According to Mali card (Marika) H. (purifying and Partial Feature of the 1994. flocculation albumen from brewer's yeast
(Purification and partial characterization of a flocculin from brewer's
Yeast method culture saccharomyces pastorianus (Saccharomyces pastorianus))), and isolate including flocculation albumen
Solution.Isolated flocculation protein solution is freeze-dried at -70 DEG C and under the conditions of 5.0 supports (Torr) to be prepared into powder.
Comparative example 1: the preparation of general yeast culture
According to Mali card (Marika) H. (purifying and Partial Feature of the 1994. flocculation albumen from brewer's yeast
(Purification and partial characterization of a flocculin frombrewer's
Yeast method culture)) saccharomyces pastorianus.Yeast culture is centrifugated with 3500rpm, and uses 0.45 μm of filtering
Film filtering supernatant is to carry out degerming.Yeast culture after degerming is freeze-dried at -70 DEG C and under the conditions of 5.0 support to prepare
At powder.
Test example 1: the expression of confirmation skin barrier factor silk polyprotein (Filaggrin)
Immortalization epidermis (HaCat) cell as Human keratinocytes strain is purchased from American type culture collection
(ATCC, Rockville, the Maryland State, the U.S.), and use is added to 10% fetal calf serum (FBS, ATCC, Rockville, Mali
Lanzhou, the U.S.) and 1% antibiotic/antifungal agent DMEM (Hai Kelong (hyclone), Lip river root, his outstanding state, the U.S.) at 37 DEG C
It is cultivated in the incubator under 5% carbon dioxide.
In order to confirm the barrier cell reinforcing effect in Human keratinocytes, by Human keratinocytes with 5x105
After the density of cells/well is inoculated into 6 hole Tissue Culture Dish, and use is added to 10% fetal calf serum and 1% antibiotic/antimycotic
The culture medium of agent is cultivated 24 hours in the incubator under 37 DEG C and 5% carbon dioxide.Comparative example 1 or embodiment 1 are added to
It in the cell of culture, then cultivates 24 hours, then recycle cell and adds 1ml TRIZOL reagent (RNA extraction reagent (RNA
), iso precious biological (TAKARA), Japan) to separate ribonucleic acid (RNA).Use instrument (Nanodrop 2000, Sai Mo
(Thermo), the U.S.) after Lai Dingliang RNA, by reacting 55 minutes and being reacted 15 minutes at 70 DEG C, Lai Hecheng at 42 DEG C
Complementary DNA (cDNA) (cDNA) (reverse transcriptase mixture (Reverse Transcriptase Mix), ELPIS biology skill
Art, South Korea).RT-PCR technology has used PCR instrument (Step One Plus, using biosystem (Applied
Biosystems), the U.S.), together with silk polyprotein primer and cDNA add Cyborg woods (SYBR Green supermix,
Using biosystem (Applied Biosystems), the U.S.), and polymerization reaction is carried out according to 40 following periods:
It carries out Polymerase activation 5 minutes at 94 DEG C, is carried out Polymerase activation 30 seconds at 95 DEG C, Polymerase activation 1 is carried out at 54 DEG C
Minute, and carried out Polymerase activation 1 minute at 72 DEG C.The base sequence of primer is as shown in table 1 below.Egg is moved by correction β-flesh
White (β-actin) gene finally analyzes the expression quantity of gene.
As a result, it was observed that the silk polyprotein in 1 addition group of embodiment is expressed compared with untreated fish group high 2.84 times (Fig. 1).
The composition that therefore, it is expected to embodiment 1 has excellent skin barrier strengthening effect.
[table 1]
Silk polyprotein primer base sequences
Test example 2: the expression of confirmation Skin Cell binding factor tight junction protein 1 (Claudin 1)
As Human keratinocytes strain immortalization epidermal cell purchased from American type culture collection (ATCC,
Rockville, the Maryland State, the U.S.), and use be added to 10% fetal calf serum (FBS, ATCC, Rockville, the Maryland State,
The U.S.) and 1% antibiotic/antifungal agent (AA, ATCC, Rockville, the Maryland State, the U.S.) DMEM culture medium (Hai Kelong
(Hyclone), Lip river root, his outstanding state, the U.S.) it is cultivated in the incubator under 37 DEG C and 5% carbon dioxide.
After Human keratinocytes are inoculated into 6 hole Tissue Culture Dish with the density of 105 cells/wells of 5x, addition is used
10% fetal calf serum and 1% antibiotic/antifungal agent culture medium are cultivated in the incubator under 37 DEG C and 5% carbon dioxide
24 hours.Comparative example 1 or embodiment 1 are added in the cell of culture, then cultivated 24 hours, cell is then recycled and adds
1ml TRIZOL reagent (RNA extracts reagent (RNA iso), precious biology (TAKARA), Japan) is to separate RNA.Use instrument
After (Nanodrop 2000, Sai Mo (Thermo), the U.S.) Lai Dingliang RNA, by being reacted 55 minutes at 42 DEG C, and at 70 DEG C
Lower reaction 15 minutes, Lai Hecheng cDNA (reverse transcriptase mixture, ELPIS biotechnology, South Korea).RT-PCR technology uses
PCR instrument (Step One Plus, using biosystem (Applied Biosystems), the U.S.), together with tight junction protein 1
Primer and cDNA add Cyborg woods (SYBR Green supermix, using biosystem (Applied together
Biosystems), the U.S.), polymerization reaction is carried out according to 40 following periods: being reacted 2 minutes at 50 DEG C, it is anti-at 95 DEG C
It should be reacted 10 seconds at 95 DEG C, and reacted 1 minute at 60 DEG C after ten minutes.The base sequence of primer is as shown in table 2 below.It is logical
Cross the expression quantity that correction beta-actin (β-actin) gene finally analyzes gene.
As a result, the expression of the tight junction protein 1 in confirmation 1 addition group of embodiment increases 1.45 times than untreated fish group.
The composition that (Fig. 2) therefore, it is expected to embodiment 1 has excellent skin moisturization.
[table 2]
1 primer base sequences of tight junction protein
Test example 3: the expression of confirmation hyaluronic acid synthetase HAS3
As Human keratinocytes strain immortalization epidermal cell purchased from American type culture collection (ATCC,
Rockville, the Maryland State, the U.S.), and use be added to 10% fetal calf serum (FBS, ATCC, Rockville, the Maryland State,
The U.S.) and 1% antibiotic/antifungal agent (AA, ATCC, Rockville, the Maryland State, the U.S.) DMEM culture medium (Hai Kelong
(Hyclone), Lip river root, his outstanding state, the U.S.) it is cultivated in the incubator under 37 DEG C and 5% carbon dioxide.
After Human keratinocytes are inoculated into 6 hole Tissue Culture Dish with the density of 105 cells/wells of 5x, and use adds
10% fetal calf serum and 1% antibiotic/antifungal agent culture medium has been added to train in the incubator under 37 DEG C and 5% carbon dioxide
It supports 24 hours.Comparative example 1 or embodiment 1 are added in the cell of culture, then cultivated 24 hours, cell is then recycled and adds
Add 1ml TRIZOL reagent (RNA extracts reagent (RNAiso), precious biology (TAKARA), Japan) to separate ribonucleic acid (RNA).
After instrument (Nanodrop 2000, Sai Mo (Thermo), the U.S.) Lai Dingliang RNA, by reacting 55 minutes at 42 DEG C simultaneously
It is reacted 15 minutes at 70 DEG C, Lai Hecheng complementary DNA (cDNA) (cDNA) (reverse transcriptase mixture (Reverse
Transcriptase Mix), ELPIS biotechnology, South Korea).RT-PCR technology has used PCR instrument, and (Step One Plus, is answered
With biosystem (Applied Biosystems), the U.S.), and added together with hyaluronic acid synthase 3 primer and cDNA
Cyborg woods (SYBR Green supermix, using biosystem (Applied Biosystems), the U.S.), and according to 40
A following period carries out polymerization reaction: carrying out Polymerase activation 5 minutes at 94 DEG C, Polymerase activation 30 is carried out at 95 DEG C
Second, it carries out Polymerase activation 1 minute at 54 DEG C, is carried out Polymerase activation 1 minute at 72 DEG C.The base sequence of primer is as follows
Shown in table 3.The expression quantity of gene is finally analyzed by correction beta-actin (β-actin) gene.
As a result, the flocculation albumen for confirming glug brewer's yeast (is implemented compared with general yeast culture (comparative example 1)
Example 1) make more hyaluronic acid synthetase expression (Fig. 3).Therefore, because increased hyaluronic acid synthetase can promote skin
The capture ability of interior moisture, it is contemplated that the composition of embodiment 1 has excellent skin moisturization.
[table 3]
Hyaluronic acid synthase 3 primer base sequences
Test example 4: the expression of confirmation exfoliating enzyme kallikrein 7 (KLK7)
As Human keratinocytes strain immortalization epidermal cell purchased from American type culture collection (ATCC,
Rockville, the Maryland State, the U.S.), and use be added to 10% fetal calf serum (FBS, ATCC, Rockville, the Maryland State,
The U.S.) and 1% antibiotic/antifungal agent DMEM culture medium (Hai Kelong (Hyclone), Lip river root, his outstanding state, the U.S.) at 37 DEG C
It is cultivated in the incubator under 5% carbon dioxide.
After Human keratinocytes are inoculated into 6 hole Tissue Culture Dish with the density of 105 cells/wells of 5x, and use adds
10% fetal calf serum and 1% antibiotic/antifungal agent culture medium has been added to train in the incubator under 37 DEG C and 5% carbon dioxide
It supports 24 hours.Comparative example 1 or embodiment 1 are added in the cell, then cultivated 24 hours, cell is then recycled and adds
1ml TRIZOL reagent (RNA extracts reagent (RNA iso), precious biology (TAKARA), Japan) is to separate ribonucleic acid (RNA).
After instrument (Nanodrop 2000, Sai Mo (Thermo), the U.S.) Lai Dingliang RNA, by being reacted 55 minutes at 42 DEG C,
It is reacted 15 minutes at 70 DEG C, Lai Hecheng complementary DNA (cDNA) (cDNA) (reverse transcriptase mixture (Reverse
Transcriptase Mix), ELPIS biotechnology, South Korea).RT-PCR technology has used PCR instrument, and (Step One Plus, is answered
With biosystem (Applied Biosystems), the U.S.), Cyborg is added together with kallikrein 7 primer and cDNA
Woods (SYBR Green supermix, using biosystem (Applied Biosystems), the U.S.), and it is as follows according to 40
Period carry out polymerization reaction: 50 DEG C react 2 minutes, 95 DEG C react 10 minutes, reacted 10 seconds at 95 DEG C, and at 60 DEG C
Lower reaction 1 minute.The base sequence of primer is as shown in table 4 below.By correction beta-actin (β-actin) gene come final point
Analyse the expression quantity of gene.
Compared with general yeast culture (comparative example 1), the flocculation albumen (embodiment 1) for confirming glug brewer's yeast makes
More exfoliating enzyme (kallikrein 7) expression.(Fig. 4) therefore, it is expected to embodiment 1 composition have make colour of skin light
Sliding and bright repair efficiency.
[table 4]
Kallikrein 7 primer base sequences
Test example 5: confirmation inhibits the effect of melanin production
B16F10 cell as the chromatophore of mouse (murine melanoma (murine melanoma)) is suspended in packet
It includes in the DMEM culture medium of 10% fetal calf serum, and after being inoculated into 6 orifice plates with the density of 1 × 105 cells/well, culture is extremely
B16F10 cell is attached to the bottom in hole.In order to induce the generation of melanin, 0.1 μM of alpha-melanocyte stimulating hormone (MSH) is handled,
Culture 3 days after being then added in every kind of sample.As the positive controls of melanin production ability test, processing
The arbutin of 100ppm.The cell of culture is washed using phosphate buffered saline solution (PBS) and is returned using trypsase (trypsin)
Receive cell.The cell that recycling is counted by using hemacytometer (hematocytometer), to each processing group with 1 ×
106 cell/mL collect the cell of identical quantity, and are centrifuged 10 minutes with 1000rpm, only to obtain cell.By that will return
The cell of receipts is 1 hour dry under 60, and the 1M sodium hydroxide including 10% dimethyl sulfoxide (DMSO) that 100 μ l are added is molten
Liquid, to obtain intracellular melanin.The melanin solution is measured by using microplate reader (microplate reader) to exist
Absorbance under 490nm, to assess the inhibiting rate of melanin production.
As a result, the flocculation albumen for confirming glug brewer's yeast (is implemented compared with general yeast culture (comparative example 1)
Example 1) it is bigger to the inhibition level of melanin production (Fig. 5).Therefore, because the composition of embodiment 1 inhibits in Skin Cell
Melanin production, therefore can have excellent whitening effect.
<110>Ke Simei poem Co., Ltd.
<120>include flocculate albumen the composition for beautifying skin
<130> PX057992PCT
<150> KR 10-2018-0004614
<151> 2018-01-12
<160> 16
<170> KoPatentIn 3.0
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ggccatctct tgctcgaagt 20
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<400> 4
gacaccttca acaccccagc 20
<210> 5
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<213>artificial sequence
<220>
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<400> 5
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gctctagaat tctcacacgt agtctttccc gct 33
<210> 7
<211> 20
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ggccatctct tgctcgaagt 20
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cttaagggtt gcttgcttgc 20
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<400> 10
gttcgtggga gatgaaggaa 20
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<213>artificial sequence
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<400> 11
ggccatctct tgctcgaagt 20
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<400> 12
gacaccttca acaccccagc 20
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<220>
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<400> 13
gcatccccga ctccaagaa 19
<210> 14
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<213>artificial sequence
<220>
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<400> 14
cagggtacct ctgcacacca a 21
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<220>
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<400> 15
ggccatctct tgctcgaagt 20
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<213>artificial sequence
<220>
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<400> 16
gacaccttca acaccccagc 20
Claims (8)
1. a kind of cosmetic composition for beautifying skin comprising flocculation albumen.
2. cosmetic composition as described in claim 1, wherein
The flocculation albumen is separated from Blastocystis bacterial strain.
3. cosmetic composition as described in claim 1, wherein
The Blastocystis bacterial strain is saccharomyces pastorianus.
4. cosmetic composition as described in claim 1 further comprises culture or the institute of the Blastocystis bacterial strain
State the extract of Blastocystis bacterial strain.
5. a kind of health functional food composition for beautifying skin comprising flocculation albumen.
6. a kind of for preventing or treating the pharmaceutical composition of skin injury or skin aging, including flocculation albumen.
7. a kind of method for treating or improving skin injury or skin aging comprising a effective amount of to individual application to include
The step of composition of flocculation albumen.
8. a kind of purposes for being used to prepare skin injury or skin aging therapeutic agent of the composition including the albumen that flocculates.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2018-0004614 | 2018-01-12 | ||
| KR1020180004614A KR102037039B1 (en) | 2018-01-12 | 2018-01-12 | Composition comprising flocculin for improving skin condition |
| PCT/KR2019/000184 WO2019139307A1 (en) | 2018-01-12 | 2019-01-07 | Composition for skin care comprising flocculin |
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| Publication Number | Publication Date |
|---|---|
| CN110267643A true CN110267643A (en) | 2019-09-20 |
| CN110267643B CN110267643B (en) | 2022-12-30 |
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| CN201980000340.5A Active CN110267643B (en) | 2018-01-12 | 2019-01-07 | Composition for skin beauty comprising flocculation protein |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR102037039B1 (en) |
| CN (1) | CN110267643B (en) |
| WO (1) | WO2019139307A1 (en) |
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| KR102426686B1 (en) * | 2021-11-04 | 2022-08-01 | (주)지에프씨생명과학 | Cosmetic Compositions for Skin Improvement Comprising Exosomes of Beluga Caviar |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110109568A (en) * | 2010-03-31 | 2011-10-06 | 주식회사 스킨푸드 | The whitening cosmetic composition using beer yeast |
| KR101807081B1 (en) * | 2015-12-22 | 2017-12-08 | 박민선 | Yeast extract derived by saccharomyces species and method for preparing thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101129239B1 (en) * | 2009-01-16 | 2012-03-26 | 주식회사 코씨드바이오팜 | Cosemtics composition containing the extract of allithiamine fermented |
-
2018
- 2018-01-12 KR KR1020180004614A patent/KR102037039B1/en active IP Right Grant
-
2019
- 2019-01-07 CN CN201980000340.5A patent/CN110267643B/en active Active
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110109568A (en) * | 2010-03-31 | 2011-10-06 | 주식회사 스킨푸드 | The whitening cosmetic composition using beer yeast |
| KR101807081B1 (en) * | 2015-12-22 | 2017-12-08 | 박민선 | Yeast extract derived by saccharomyces species and method for preparing thereof |
Non-Patent Citations (2)
| Title |
|---|
| GENBANK:BAG49462.1: "flocculin [Saccharomyces pastorianus]", 《GENBANK》 * |
| MARIKA H. STRAVER等: "Purification and Partial Characterization of a Flocculin from Brewer’s Yeast", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 * |
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| WO2019139307A1 (en) | 2019-07-18 |
| KR102037039B1 (en) | 2019-10-28 |
| KR20190086280A (en) | 2019-07-22 |
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