CN110257273A - A kind of tobacco waste fermenting agent and its application method - Google Patents
A kind of tobacco waste fermenting agent and its application method Download PDFInfo
- Publication number
- CN110257273A CN110257273A CN201910363817.8A CN201910363817A CN110257273A CN 110257273 A CN110257273 A CN 110257273A CN 201910363817 A CN201910363817 A CN 201910363817A CN 110257273 A CN110257273 A CN 110257273A
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- China
- Prior art keywords
- tobacco waste
- fermenting agent
- waste fermenting
- tobacco
- application method
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The present invention is suitable for fermenting agent technical field, a kind of tobacco waste fermenting agent and its application method are provided, it is compounded to obtain tobacco waste fermenting agent by streptomyces griseus, penicillium oxalicum, bacillus subtilis, bacillus licheniformis, aspergillus niger, it can effectively improve the decomposition efficiency and quality of tobacco waste after addition tobacco waste, the high temperature of cooperation fermentation efficiently kills original tape microorganism such as tobacco mosaic virus (TMV) and root-knot nematode egg, improves the humus quality generated after tobacco waste processing.
Description
Technical field
The invention belongs to fermenting agent technical field more particularly to a kind of tobacco waste fermenting agent and its application sides
Method.
Background technique
Tobacco waste is the part passed into disuse in tobacco leaf production, and traditional processing method is to burn, and has both wasted money
Source polluted environment again, while a large amount of illegal retailers manufacture fake and forged cigarette using tobacco waste, disrupt the market, invade
Evil consumer rights.Containing the nutritional ingredients such as a large amount of amino acid, organic matter in tobacco waste, can effective fertilizing soil, together
When contain nicotine again, it is harmful to have the function of that expelling parasite is kept away, can decompose in the soil, will not remain.It is in the art mostly by cigarette at present
Careless waste carries out fermentation process and green manure is made or the raw material as biomass fuel is processed.But tobacco is discarded
Cigarette pole fiber element and content of lignin in object is very high, not hygroscopic, not easy to break, and hard to be difficult to soften, decomposed degree is not high.
Summary of the invention
In view of the above problems, the purpose of the present invention is to provide a kind of tobacco waste fermenting agent and its application method,
It is intended to provide a kind of using tobacco waste as the microbial inoculum of main production raw material fermentation organic fertilizer, point of raising raising tobacco waste
Solve efficiency and quality.
A kind of tobacco waste fermenting agent, by streptomyces griseus 47~51%, penicillium oxalicum 10~15%, withered grass bud
Spore bacillus 8~12%, bacillus licheniformis 8~12%, aspergillus niger 15~20%, mixed after obtain tobacco waste fermentation
Microbial inoculum.
Specifically, the streptomyces griseus PDA culture medium, inoculum concentration is to train under 5~10%, 28~37 DEG C of aerobic conditions
It supports 2~3 days.
Specifically, the penicillium oxalicum and aspergillus niger PDA culture medium, inoculum concentration is 3~5%, 20~25 DEG C aerobic
Under the conditions of cultivate 3~4 days.
Specifically, bacillus subtilis and bacillus licheniformis LB culture medium, inoculum concentration is 5~10%, 30~37 DEG C
It is cultivated 1~2 day under aerobic condition.
On the other hand, a kind of application method of tobacco waste fermenting agent, includes the following steps:
A, base-material is prepared, 60~75% tobacco waste, 25~40% turfs, moisture content 25 are contained in the base-material
~40%, pH value is maintained between 6~8；
B, the tobacco waste fermenting agent is inoculated in Xiang Suoshu base-material, inoculum concentration is the 0.7~1% of matrix weight,
Then start to ferment, oxygen flux is paid attention in fermentation process.
Specifically, the fermentation time be 20~for 24 hours.
A kind of tobacco waste fermenting agent and its application method provided by the invention, by streptomyces griseus, penicillium oxalicum
Bacterium, bacillus subtilis, bacillus licheniformis, aspergillus niger are compounded to obtain tobacco waste fermenting agent, can effectively be mentioned
The decomposition efficiency and quality of high tobacco waste cooperate the high temperature of fermentation to original tape microorganism such as tobacco mosaic virus (TMV) and root knot line
Worm's ovum is efficiently killed, and the humus quality generated after tobacco waste processing is improved.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
A kind of tobacco waste fermenting agent, by streptomyces griseus 47~51%, penicillium oxalicum 10~15%, withered grass bud
Spore bacillus 8~12%, bacillus licheniformis 8~12%, aspergillus niger 15~20%, mixed after obtain tobacco waste fermentation
Microbial inoculum.
The streptomyces griseus PDA culture medium, inoculum concentration are that 2~3 are cultivated under 5~10%, 28~37 DEG C of aerobic conditions
It.The penicillium oxalicum and aspergillus niger PDA culture medium, inoculum concentration be culture 3 under 3~5%, 20~25 DEG C of aerobic conditions~
4 days.Bacillus subtilis and bacillus licheniformis LB culture medium, inoculum concentration.
Claims (6)
1. a kind of tobacco waste fermenting agent, which is characterized in that by streptomyces griseus 47~51%, penicillium oxalicum 10~
15%, bacillus subtilis 8~12%, bacillus licheniformis 8~12%, aspergillus niger 15~20%, mixed after obtain cigarette
Careless waste fermenting bacterial preparation.
2. a kind of tobacco waste fermenting agent as described in claim 1, which is characterized in that the streptomyces griseus PDA
Culture medium, inoculum concentration are to cultivate 2~3 days under 5~10%, 28~37 DEG C of aerobic conditions.
3. a kind of tobacco waste fermenting agent as described in claim 1, which is characterized in that the penicillium oxalicum and black song
Mould to use PDA culture medium, inoculum concentration is to cultivate 3~4 days under 3~5%, 20~25 DEG C of aerobic conditions.
4. a kind of tobacco waste fermenting agent as described in claim 1, which is characterized in that bacillus subtilis and lichens bud
Spore bacillus LB culture medium, inoculum concentration are to cultivate 1~2 day under 5~10%, 30~37 DEG C of aerobic conditions.
5. a kind of application method of tobacco waste fermenting agent, which comprises the steps of:
A, it preparing base-material, contains 60~75% tobacco waste, 25~40% turfs in the base-material, moisture content is 25~
40%, pH value is maintained between 6~8；
B, the tobacco waste fermenting agent is inoculated in Xiang Suoshu base-material, inoculum concentration is the 0.7~1% of matrix weight, then
Start to ferment, oxygen flux is paid attention in fermentation process.
6. a kind of application method of tobacco waste fermenting agent, which is characterized in that the fermentation time be 20~for 24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910363817.8A CN110257273A (en) | 2019-04-30 | 2019-04-30 | A kind of tobacco waste fermenting agent and its application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910363817.8A CN110257273A (en) | 2019-04-30 | 2019-04-30 | A kind of tobacco waste fermenting agent and its application method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110257273A true CN110257273A (en) | 2019-09-20 |
Family
ID=67914096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910363817.8A Pending CN110257273A (en) | 2019-04-30 | 2019-04-30 | A kind of tobacco waste fermenting agent and its application method |
Country Status (1)
| Country | Link |
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| CN (1) | CN110257273A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110304970A (en) * | 2019-04-30 | 2019-10-08 | 江苏思威博生物科技有限公司 | A kind of fermenting crops straws microbial inoculum and its application method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102887754A (en) * | 2012-11-05 | 2013-01-23 | 师宗海利食品有限责任公司 | Process for producing marsh gas and bioorganic fertilizer by utilizing tobacco waste |
| CN103571749A (en) * | 2012-08-09 | 2014-02-12 | 河南省恒隆态生物工程股份有限公司 | AT fermentation medium for treating tobacco wastes and preparation method thereof |
-
2019
- 2019-04-30 CN CN201910363817.8A patent/CN110257273A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103571749A (en) * | 2012-08-09 | 2014-02-12 | 河南省恒隆态生物工程股份有限公司 | AT fermentation medium for treating tobacco wastes and preparation method thereof |
| CN102887754A (en) * | 2012-11-05 | 2013-01-23 | 师宗海利食品有限责任公司 | Process for producing marsh gas and bioorganic fertilizer by utilizing tobacco waste |
Non-Patent Citations (3)
| Title |
|---|
| 刘子仪等: "发酵烟草废弃物制备有机肥的功能真菌筛选及发酵条件优化", 《云南农业科技》 * |
| 张占军等: "烟草废弃物腐熟发酵生物有机肥效果试验", 《试验研究》 * |
| 杨宗灿等: "烟叶中纤维素高效降解菌的筛选与作用效果研究", 《河南农业大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110304970A (en) * | 2019-04-30 | 2019-10-08 | 江苏思威博生物科技有限公司 | A kind of fermenting crops straws microbial inoculum and its application method |
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Application publication date: 20190920 |