CN110257244B

CN110257244B - Incubator

Info

Publication number: CN110257244B
Application number: CN201910326904.6A
Authority: CN
Inventors: 刘锡玲; 毕路; 代娟; 黄双龙; 李佳
Original assignee: Wuhan Guanrui Biotechnology Co ltd
Current assignee: Wuhan Guanrui Biotechnology Co ltd
Priority date: 2019-04-23
Filing date: 2019-04-23
Publication date: 2023-05-23
Anticipated expiration: 2039-04-23
Also published as: CN110257244A

Abstract

The invention discloses an incubator, and belongs to the field of incubators. The incubator includes casing, heating element and baffle, and heating element and baffle all are located the casing, and heating element is including controller and the hot plate that the electricity is connected together, and controller fixed mounting is in the bottom of casing, and baffle fixed mounting is at the top of casing, and hot plate fixed mounting is on the bottom surface of baffle, and hot plate and controller interval arrangement are provided with the jack that is used for cartridge reagent pipe and the recess that is used for shelving reagent card on the top surface of baffle, jack and recess interval arrangement. The invention can realize isothermal amplification of nucleic acid and simultaneous incubation of samples required by colloidal gold immunochromatography.

Description

Incubator

Technical Field

The invention belongs to the field of incubators, and particularly relates to an incubator.

Background

The isothermal nucleic acid amplification and the colloidal gold immune technology are combined, and the isothermal nucleic acid amplification method is used for amplification, and the colloidal gold labeling technology is combined. Carboxyl Fluorescein (FAM) marked specific probes are specifically combined with Biotin (Biotin) marked nucleic acid amplification products, and the carboxyl Fluorescein (FAM) marked specific probes are dripped on a test paper strip to be combined with colloidal gold marked anti-FAM antibodies to form ternary complexes, and when the ternary complexes are diffused to a detection line, the ternary complexes are captured by Biotin ligands to form the detection line; the unhybridized FAM-labeled probe is combined with the anti-FAM antibody labeled with colloidal gold to form a biotin-free binary complex, which is combined on a quality control line.

By combining the nucleic acid isothermal amplification technology and the colloidal gold immunochromatography technology, the detection is easier to judge, the method has the advantages of short time consumption, simplicity and convenience in operation and visual and accurate result judgment, and has wide application prospects in primary clinical rapid detection.

In carrying out the invention, the inventors have found that the prior art has at least the following problems:

in the present stage, in order to realize the combination of the isothermal amplification of nucleic acid and the colloidal gold immunochromatography, two different temperature control instruments are adopted in the operation, and the method has the advantages of complex operation, high price, inconvenience in carrying and difficulty in realizing the rapid clinical detection of a basic layer.

Disclosure of Invention

The embodiment of the invention provides an incubator which can incubate samples required by isothermal nucleic acid amplification and colloidal gold immunochromatography. The technical scheme is as follows:

the embodiment of the invention provides an incubator, which comprises a shell, a heating component and a partition plate, wherein the heating component and the partition plate are both positioned in the shell, the heating component comprises a controller and a heating plate which are electrically connected together, the controller is fixedly arranged at the bottom of the shell, the partition plate is fixedly arranged at the top of the shell, the heating plate is fixedly arranged on the bottom surface of the partition plate, the heating plate and the controller are arranged at intervals, the top surface of the partition plate is provided with a jack for inserting a reagent tube and a groove for placing a reagent card, and the jack and the groove are arranged at intervals.

In one implementation of the invention, the controller includes a power source fixedly mounted on the bottom plate of the housing and an electrical control board fixedly mounted on the side wall of the housing, the electrical control board being electrically connected to the power source.

In another implementation mode of the invention, a plurality of hooks are arranged on the inner top edge of the shell, the hooks are circumferentially spaced along the inner top edge of the shell, and the partition plate is fixedly mounted on the hooks.

In yet another implementation of the present invention, the hook includes a support arm and a connection arm, the connection arm is arranged perpendicular to the bottom plate of the housing, one end of the connection arm is fixedly connected with the top inner edge of the housing, the other end of the connection arm is fixedly connected with the support arm, the support arm is arranged parallel to the bottom plate of the housing, and the support arm is fixedly mounted with the bottom surface of the partition.

In yet another implementation of the present invention, the top surface of the partition is provided with a boss protruding from the top surface of the partition, and the insertion hole is provided on one surface of the boss protruding from the partition.

In yet another implementation of the present invention, the incubator further includes a tray, a plurality of slots matched with the reagent cards are provided on a top surface of the tray, the slots are arranged at intervals, and a bottom surface of the tray is placed in the groove.

In yet another implementation of the present invention, the edge of the tray is provided with a first notch, the edge of the groove is provided with a second notch, and the first notch and the second notch are arranged opposite to each other.

In a further implementation of the invention, the inner wall of the receptacle is provided with a rubber ring, which is arranged coaxially with the receptacle, the inner bore of which matches the outer wall of the reagent tube.

In yet another implementation of the present invention, the side plate of the housing is provided with heat dissipation holes.

In yet another implementation of the present invention, a flip cover is provided on top of the housing, the flip cover being hinged with the housing, the flip cover being covered on the partition.

The technical scheme provided by the embodiment of the invention has the beneficial effects that:

when the incubator provided by the embodiment of the invention incubates samples required by nucleic acid isothermal amplification and colloidal gold immunochromatography, a reagent tube can be inserted into the jack, and a reagent card is placed in the groove. And then the controller controls the heating plate to generate heat, so that the simultaneous heating incubation of the reagent tube and the reagent card can be realized. And, because the hot plate and the controller interval arrangement, so the heat that the hot plate produced can not cause the influence to the controller, has improved the reliability of incubator.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is an exploded view of an incubator provided by an embodiment of the present invention;

FIG. 2 is a cross-sectional view of an incubator provided by an embodiment of the present invention;

FIG. 3 is a schematic view of a housing according to an embodiment of the present invention;

the symbols in the drawings are as follows:

1. a housing; 11. a heat radiation hole; 12. a flip cover; 13. a window; 14. a button; 15. a hook; 151. a support arm; 152. a connecting arm; 2. a heating assembly; 21. a controller; 211. a power supply; 212. an electric control board; 213. a display screen; 214. a circuit board; 22. a heating plate; 3. a partition plate; 31. a jack; 311. a rubber ring; 32. a groove; 33. a boss; 34. a second notch; 4. a tray; 41. a clamping groove; 42. a first notch; 100. a reagent tube; 200. a reagent card.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, the embodiments of the present invention will be described in further detail with reference to the accompanying drawings.

The embodiment of the invention provides an incubator, as shown in fig. 1, the incubator comprises a shell 1, a heating component 2 and a partition plate 3, wherein the heating component 2 and the partition plate 3 are positioned in the shell 1, the heating component 2 comprises a controller 21 and a heating plate 22 which are electrically connected together, the controller 21 is fixedly arranged at the bottom of the shell 1, the partition plate 3 is fixedly arranged at the top of the shell 1, the heating plate 22 is fixedly arranged on the bottom surface of the partition plate 3, the heating plate 22 and the controller 21 are arranged at intervals (see fig. 2), a jack 31 for inserting a reagent tube 100 and a groove 32 for placing a reagent card 200 are arranged on the top surface of the partition plate 3, and the jack 31 and the groove 32 are arranged at intervals.

When the incubator provided by the embodiment of the invention incubates samples required by isothermal amplification of nucleic acid and colloidal gold immunochromatography, the reagent tube 100 can be inserted into the insertion hole 31, and the reagent card 200 is placed in the groove 32. Then, the controller 21 controls the heating plate 22 to generate heat, so that the heating plate 22 heats the partition plate 3, and the partition plate 3 can heat and incubate the reagent tube 100 and the reagent card 200 simultaneously. In addition, since the heating plate 22 and the controller 21 are arranged at intervals, the heat generated by the heating plate 22 does not affect the controller 21, and the reliability of the incubator is improved.

In the present embodiment, the side plate of the housing 1 is provided with the heat radiation hole 11.

In the above implementation, when the heating plate 22 is operated, heat is generated, and an excessive amount of heat may damage electronic components such as the controller 21. The arrangement of the heat dissipation holes 11 can effectively avoid the overhigh temperature in the shell 1.

Illustratively, the heat dissipation apertures 11 are disposed on the housing 1 in a position relative to the controller 21.

In this embodiment, the top of the housing 1 is provided with a flip 12, the flip 12 is hinged with the housing 1, and the flip 12 covers the partition 3.

In the above implementation, the flip cover 12 is used to provide protection and thermal insulation effects for the reagent tube 100 and the reagent card 200, and improve the reliability of the incubator.

Optionally, the outer edge of the flip cover 12 and the top outer edge of the housing 1 are provided with mating spigots, and when the flip cover 12 and the housing 1 are assembled together, the spigots of the flip cover 12 and the housing 1 are fit together in a mutually mating manner, thereby improving the stability between the flip cover 12 and the housing 1.

Illustratively, both the flip cover 12 and the housing 1 may be plastic structural members, thereby reducing the overall weight of the incubator and improving portability of the incubator.

With continued reference to fig. 1, in the present embodiment, the controller 21 includes a power supply 211 and an electric control board 212, the power supply 211 is fixedly mounted on the bottom plate of the housing 1, the electric control board 212 is fixedly mounted on the side wall of the housing 1, and the electric control board 212 and the power supply 211 are electrically connected.

In the above implementation, the power supply 211 is used to supply power to the heating plate 22, and the electronic control board 212 is used to receive the instruction of the user and control the power supply 211. For example, the electric control board 212 can adjust the output power of the power supply 211, so as to control the heating value of the heating plate 22.

Illustratively, the electronic control board 212 may include a display screen 213 and a circuit board 214, the display screen 213 being disposed on the circuit board 214, a window 13 being disposed on a side wall of the housing 1, and the display screen 213 being embedded in the window 13. The circuit board 214 is provided with contacts, the side wall of the shell 1 is provided with buttons 14, and the buttons 14 are arranged in one-to-one correspondence with the contacts.

In the above implementation, the circuit board 214 is used to carry the display screen 213 and to receive instructions for input via the buttons 14. A display 213 is embedded in the window 13 and may provide feedback to the user, such as incubation temperature, incubation time, etc.

Fig. 3 is a schematic view of the structure of the housing, and in combination with fig. 3, in this embodiment, a plurality of hooks 15 are provided at the top inner edge of the housing 1, each hook 15 is circumferentially spaced along the top inner edge of the housing 1, and the partition plate 3 is fixedly mounted on the hooks 15.

In the above described implementation, the hooks 15 are used to provide a mounting base for the partition 3 so that the partition 3 can be fixedly mounted on the housing 1. Moreover, since the heating plate 22 is fixedly arranged on the partition plate 3, the partition plate 3 is arranged on the hook 15, so that the partition plate 3 and the heating plate 22 are arranged at intervals from the controller 21, and the reliability of the incubator is further ensured.

Alternatively, the hanger 15 includes a support arm 151 and a connection arm 152, the connection arm 152 is arranged perpendicular to the bottom plate of the housing 1, one end of the connection arm 152 is fixedly connected with the top inner edge of the housing 1, the other end of the connection arm 152 is fixedly connected with the support arm 151, the support arm 151 is arranged parallel to the bottom plate of the housing 1, and the support arm 151 is fixedly mounted with the bottom surface of the partition 3.

In the above-described implementation, the support arm 151 abuts against the bottom surface of the partition board 3, so that the rest of the partition board 3 can be achieved.

Illustratively, the supporting arm 151 is provided with a through hole for inserting a screw, and the partition 3 and the supporting arm 151 are fixedly mounted together by the screw.

In the above-described implementation manner, the support arm 151 and the partition plate 3 are mounted by screws, so that the detachable mounting between the support arm 151 and the partition plate 3 can be reliably achieved.

Referring again to fig. 1, in the present embodiment, the top surface of the partition board 3 is provided with a boss 33, the boss 33 protrudes from the top surface of the partition board 3, and the insertion hole 31 is provided on one surface of the boss 33 protruding from the partition board 3.

In the above-described implementation, the boss 33 is provided on the top surface of the partition board 3, so as to increase the length of the insertion hole 31, so that the reagent tube 100 can be more firmly inserted into the insertion hole 31.

Optionally, the inner wall of the receptacle 31 is provided with a rubber ring 311 (see fig. 2), the rubber ring 311 being arranged coaxially with the receptacle 31, the inner bore of the rubber ring 311 matching the outer wall of the reagent vessel 100.

In the above implementation manner, when the reagent tube 100 is inserted into the insertion hole 31, the reagent tube 100 is inserted into the inner hole of the rubber ring 311, and the rubber ring 311 can improve the insertion stability of the reagent tube 100, avoid the unnecessary shake of the reagent tube 100, and improve the reliability of the incubator.

Illustratively, the inner hole of the rubber ring 311 may be provided with a bump, thereby further improving the friction between the rubber ring 311 and the reagent tube 100, so that the reagent tube 100 may be more firmly inserted into the rubber ring 311.

With continued reference to fig. 1, in this embodiment, the incubator further comprises a tray 4, wherein a plurality of slots 41 matching the reagent cards 200 are provided on the top surface of the tray 4, the slots 41 are spaced apart, and the bottom surface of the tray 4 rests in the recess 32.

In the above-described embodiment, all of the reagent cards 200 may be disposed on the tray 4, so that the plurality of reagent cards 200 may be moved at once by taking and putting the tray 4, thereby improving the operation efficiency.

Optionally, the edge of the tray 4 is provided with a first notch 42, the edge of the groove 32 is provided with a second notch 34, and the first notch 42 and the second notch 34 are arranged opposite to each other.

In the above implementation, the first notch 42 and the second notch 34 are disposed opposite to each other, so that a space for accommodating the fingers of the operator can be formed, thereby facilitating the taking of the tray 4 from the partition 3 and improving operability.

The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.

Claims

1. An incubator, characterized in that the incubator comprises a shell, a heating component and a baffle, wherein a plurality of hooks are arranged at the inner edge of the top of the shell, each hook is circumferentially and alternately arranged along the inner edge of the top of the shell, the baffle is fixedly arranged on the hooks, the hooks comprise a supporting arm and a connecting arm, the connecting arm is arranged perpendicular to the bottom surface of the shell, one end of the connecting arm is fixedly connected with the inner edge of the top of the shell, the other end of the connecting arm is fixedly connected with the supporting arm, the supporting arm is arranged parallel to the bottom surface of the shell, the supporting arm is fixedly arranged with the bottom surface of the baffle, the heating component and the baffle are both positioned in the shell, the heating component comprises a controller and a heating plate which are electrically connected together, the controller is fixedly arranged at the bottom of the shell, the baffle is fixedly arranged at the top of the shell, the heating plate is fixedly arranged on the bottom surface of the baffle, and the connecting arm is alternately arranged with a groove for inserting a reagent card and a reagent card, and the groove for inserting the reagent is alternately arranged on the top surface of the baffle;

the top surface of the partition board is provided with a boss, the boss protrudes out of the top surface of the partition board, the inserting hole is formed in one surface of the boss protruding out of the partition board, the boss is close to the end part of the partition board, so that the orthographic projection of the inserting hole on the plane where the heating plate is located is positioned outside the heating plate, and the boss and the partition board are an integrated structural member;

the inner wall of the jack is provided with a rubber ring, the rubber ring and the jack are coaxially arranged, an inner hole of the rubber ring is matched with the outer wall of the reagent tube, and the inner hole of the rubber ring is provided with a bump;

the heating assembly is configured to control the heating plate to generate heat through the controller, so that the heating plate heats the partition plate, and the partition plate heats and incubates the reagent tube and the reagent card simultaneously.

2. The incubator of claim 1, wherein the controller comprises a power source fixedly mounted on the bottom plate of the housing and an electrical control board fixedly mounted on the side wall of the housing, the electrical control board being electrically connected to the power source.

3. The incubator of claim 1, further comprising a tray having a plurality of slots on a top surface thereof for mating with the reagent cards, the slots being spaced apart, a bottom surface of the tray resting within the recess.

4. An incubator as claimed in claim 3 wherein the tray has a first notch at an edge thereof and a second notch at an edge thereof, the first and second notches being oppositely disposed.

5. The incubator of any one of claims 1 to 4, wherein the side plates of the housing are provided with heat dissipation holes.

6. The incubator of any one of claims 1-4, wherein a flip cover is provided on top of the housing, the flip cover being hinged to the housing, the flip cover being over the partition.