CN110251984B - Efficient trehalose extraction equipment and extraction method thereof - Google Patents

Abstract

The invention discloses a high-efficiency trehalose extraction device and an extraction method thereof, and the device comprises a lifting module, a push-out module, a main body module and a collection module, wherein the main body module comprises a material box with an opening at the upper end, the bottom of the inner side of the material box is provided with a constant-temperature heating sheet, the left side wall of the material box is connected with a feeding pipe, left and right heating box parts are symmetrically fixed on the left and right side surfaces in the material box, the left and right outer side walls of the material box are respectively provided with an exhaust fan with an air pipe positioned in the material box, the lifting module comprises a single-shaft driver bracket provided with a vertical single-shaft driver, the push-out module comprises a cylinder horizontally and transversely arranged, and the collection module comprises a: the trehalose crystal can also be avoided traditional extraction equipment long-term operation to lead to the crystallization to block up the problem of discharge gate to can not only high-efficiently extract out.

Description

Efficient trehalose extraction equipment and extraction method thereof

Technical Field

The invention belongs to the technical field of trehalose extraction, and particularly relates to high-efficiency trehalose extraction equipment and an extraction method thereof.

Background

Trehalose is a non-reducing disaccharide composed of two glucose molecules bonded via an α, α -1,1 bond, and is widely found in bacteria, fungi, algae, lower plants and insects. The research shows that the sugar has unique biological function, has the functions of protecting biological macromolecules, protecting cell membranes and protecting proteins from being damaged by freezing, drying, osmotic pressure change and the like, and has wide application in the fields of food, medicine, cosmetics, agriculture and the like.

At present, the existing trehalose preparation method comprises the following steps: inoculating an enzyme-producing strain into a fermentation medium to prepare a fermentation liquid, separating the fermentation liquid by thalli, breaking the cell wall of the thalli, purifying the enzyme to prepare a trehalose synthase solution, carrying out cross-linking embedding reaction on the trehalose synthase solution to prepare an immobilized trehalose synthase, mixing the immobilized trehalose synthase with a maltose substrate solution, and carrying out conversion, separation and crystallization to prepare trehalose.

The trehalose extraction equipment comprises enzyme bacterium culture equipment, enzyme bacterium fermentation equipment, thallus wall breaking equipment, synthetic fluid centrifugal equipment, crosslinking embedding reaction equipment, synthetic enzyme and substrate mixing and stirring equipment, mixed solution separation and filtration equipment and a drying and crystallization device.

However, trehalose drying and crystallizing devices of companies are different, and have the problems of unstable content, irregular crystal form, poor fluidity, caking, poor purity, long crystallizing time and the like, and when a traditional crystallizing device works, a crystal part is continuously precipitated at the bottom of crystallizing equipment along with reaction, the crystal is easy to harden after long-term operation, so that the discharging is very difficult and even a discharging port is blocked, the regular shutdown for cleaning or the reduction of reaction concentration is needed to wash out the crystals at the discharging port, and the crystallizing efficiency is low, so that a novel trehalose extracting device and a novel trehalose extracting method are urgently needed to be found to solve the problems.

Disclosure of Invention

The invention provides high-efficiency trehalose extraction equipment and an extraction method thereof, which not only can efficiently extract trehalose crystals, but also can avoid the problem that a discharge port is blocked by crystallization due to long-term operation of the traditional extraction equipment.

In order to solve the technical problems, the invention adopts the following technical scheme:

a trehalose extraction equipment comprises a drying and crystallizing device, wherein the drying and crystallizing device comprises a lifting module, a pushing module, a main body module and a collecting module; the main body module comprises a material box with an opening at the upper end, a constant temperature heating sheet is arranged at the bottom of the inner side of the material box, a feeding pipe is connected with the left side wall of the material box, a left heating box part and a right heating box part are symmetrically fixed on the left side surface and the right side surface in the material box, the left heating box part comprises four left heating boxes which are sequentially fixed on the left side surface in the material box from top to bottom, the right heating box part comprises four right heating boxes, the left heating box at the lowest side is positioned above the feeding pipe, the left outer side wall and the right outer side wall of the material box are respectively provided; the lifting module comprises a single-shaft driver support provided with a vertical single-shaft driver, a drying box connecting rod which is vertical and can move up and down under the driving of the single-shaft driver is arranged on the single-shaft driver, a drying box module is fixed at the bottom end of the drying box connecting rod and can move up and down in a material box under the driving of the single-shaft driver along with the drying box connecting rod, a plurality of drying chambers are arranged in the drying box module from top to bottom, and water absorbing cloth is fixed on the top and bottom surfaces in each drying chamber; the pushing module comprises a cylinder which is horizontally and transversely arranged, a push rod of the cylinder is rightward, a fixed block is fixedly connected with the right end of the push rod, a plurality of scraper fixing rods which are horizontally and transversely arranged are fixed at the right end of the fixed block from top to bottom, scrapers are fixedly connected with the right end of each scraper fixing rod, the number of the scrapers and the corresponding scraper fixing rods is consistent with that of the drying chambers, and the structures and the sizes of the scrapers are matched with those of the corresponding drying chambers; the main part module right side is equipped with collection module, and collection module is equipped with the collecting box including being used for collecting from the collecting plate that absorbs water the trehalose crystal of scraping off on the cloth, collecting plate right side below.

Further, the heating temperatures of the four left heating boxes from top to bottom were controlled to 50 ℃, 55 ℃, 60 ℃ and 70 ℃ in this order, and the heating temperature of the constant temperature heating plate was controlled to 80 ℃.

Furthermore, the single-shaft driver support is vertically arranged on the ground and is a U-shaped frame body with an opening at the left part, the right part and the lower part, the single-shaft driver support comprises a vertical front support, a vertical rear support and a horizontal top support, the single-shaft driver support is integrally erected at the upper part of the feed box, the front support is arranged at the front side of the front side wall of the feed box, the rear support is arranged at the rear side of the rear side wall of the feed box, a horizontal beam longitudinally arranged between the front support and the rear support is arranged at the relative position of the upper part of the single-shaft driver support, the beam is positioned above the feed box, the vertical support is arranged between the middle part of the upper surface of the beam and the middle; install single-axis drive slip table and single-axis drive slip table free movement from top to bottom in single-axis drive stroke range on the single-axis drive, single-axis drive slip table right flank is fixed with the positive center of the drying cabinet connecting rod of vertical arrangement and drying cabinet connecting rod bottom fixed connection drying cabinet module top surface, and drying cabinet connecting rod and all follow single-axis drive slip table free movement from top to bottom with drying cabinet connecting rod fixed connection's drying cabinet module.

Furthermore, the drying box module comprises a rectangular drying box frame body which is horizontally arranged and has an opening at the left end and the right end, a plurality of horizontally and transversely arranged drying box partition plates are arranged in the drying box frame body from top to bottom, the front ends of the drying box partition plates are fixedly connected with the front side surface in the drying box frame body, the rear ends of the drying box partition plates are fixedly connected with the rear side surface in the drying box frame body, the left end and the right end of each drying box partition plate are respectively flush with the left end and the right end of the drying box frame body, the drying box partition plates are uniformly distributed along the vertical direction so as to divide the inside of the drying box into a plurality of drying chambers from top to bottom, the plurality of drying chambers have the same structure, water absorption cloth is respectively and fixedly paved on the top surface and the bottom surface in each drying chamber, a certain gap is left between the lower surface of the water absorption cloth on the top surface of the drying chamber and the upper surface of the water absorption cloth on the bottom surface of, so as to scrape the lower surface of the water absorbing cloth on the top surface of the corresponding drying chamber and the upper surface of the water absorbing cloth on the bottom surface of the corresponding drying chamber simultaneously.

Furthermore, each left heating box is internally provided with a left electric heating wire which controls the distance between the left heating box component and the right heating box component of the corresponding left heating box under the control of the left electric heating wire and the left electric heating wire to be matched with the horizontal transverse distance of the frame body of the drying box, and the left heating box is a rectangular box with an opening at the right side and is matched with the openings at the left side and the right side of the drying chamber in size and shape.

Further, the fixed block is vertical arranging, the shape of each scraper blade dead lever, the structure, the size is all the same, the shape of each scraper blade, the structure, the size is all the same, and the scraper blade is vertical and arranges along the fore-and-aft direction, each scraper blade top, the bottom respectively with the cloth lower surface that absorbs water of corresponding drying chamber top surface, the cloth upper surface contact that absorbs water of corresponding drying chamber bottom surface, each scraper blade is free movement about in corresponding drying chamber simultaneously, scraper blade dead lever length surpasss the horizontal transverse length of drying chamber and the diameter is less than corresponding drying chamber vertical height, vertical clearance between the adjacent scraper blade and the vertical thickness looks adaptation of drying cabinet cut-off board.

Furthermore, the top end of the right side wall of the material box is fixedly provided with a collecting plate which is obliquely arranged, the left side of the collecting plate is high and the right side of the collecting plate is low, a collecting box with an upper opening is arranged under the right end of the collecting plate, each scraper blade enters the corresponding drying chamber to scrape materials when the drying box frame body rises to the highest position, and the left end of the collecting plate is positioned at the right lower part of the drying box frame body when the collecting plate rises to the highest position.

Furthermore, the feed box is a rectangular box body horizontally placed on the ground, the heating sheet is arranged at the bottom of the inner side of the feed box and protrudes inwards along the peripheral wall, the size of the space of the inner side of the heating sheet is larger than that of the drying box module, the heating sheet is formed by an electric heating wire, and the electric heating wire in the heating sheet controls the heating temperature of the heating sheet; the structures and the positions of the exhaust fans on the left side wall and the right side wall are symmetrical; the cylinder is fixedly connected with the material box through an L-shaped cylinder bracket.

Further, the extraction method of the high-efficiency trehalose extraction equipment is characterized by comprising the following steps: firstly, inoculating an enzyme-producing strain into a fermentation culture medium to prepare a fermentation liquid; separating the obtained fermentation liquor by thalli, breaking cell walls and purifying enzyme to obtain trehalose synthase solution; carrying out cross-linking embedding reaction on trehalose synthetase liquid and chitosan gel to prepare immobilized trehalose synthetase; mixing the immobilized trehalose synthetase and a maltose substrate solution, and preparing a trehalose solution through conversion and separation; then, the trehalose solution is dried and crystallized by using the high-efficiency trehalose extraction equipment, and the drying and crystallization steps are as follows:

s1, conveying the processed trehalose solution into a material box through a feeding pipe, enabling the liquid level of the trehalose solution to be lower than the height of the feeding pipe, starting a heating sheet, controlling the heating temperature of the heating sheet to be 80 ℃, heating the trehalose solution by the heating sheet, starting a single-shaft driver to enable a sliding table of the single-shaft driver to descend and drive a connecting rod of a drying box to descend, driving a module of the drying box to descend by the connecting rod of the drying box, when the sliding table of the single-shaft driver descends to a stroke bottom dead center, completely sinking the module of the drying box into the trehalose solution in a heating state, and absorbing the trehalose solution by water absorption cloth in the drying chamber;

s2, after a period of time, the drying box module slowly rises along with the single-shaft driver sliding table, the excessive moisture absorbed by the water cloth falls back to the trehalose solution at the bottom of the material box again when the single-shaft driver sliding table rises, the drying box module stops rising when the drying box module rises to enable the uppermost layer drying chamber of the drying box module to correspond to the lowermost layer left heating box at the inner side of the material box, the left heating box, the uppermost layer drying chamber and the right heating box form a relatively closed box body together, then all the left heating box and the right heating box are started, and the heating temperatures of the four left heating boxes from top to bottom are controlled to be 50 ℃, 55 ℃, 60 ℃ and 70 ℃ in sequence, so that the uppermost layer drying chamber stays for a period of time in an environment of 70 ℃;

s3, after a period of time, the drying oven module continuously and slowly rises along with the single-shaft driver sliding table, so that the drying chamber on the uppermost layer of the drying oven module corresponds to the second layer of left heating boxes counting from bottom to top on the inner side of the material box, and the drying oven module stops rising when the second layer of drying chambers from top to bottom corresponds to the left heating box on the lowest layer on the inner side of the material box, so that the drying chamber on the uppermost layer stays for a period of time in an environment of 60 ℃;

s4, after a period of time, the drying oven module continuously and slowly rises along with the single-shaft driver sliding table, so that the drying oven module stops rising when the uppermost drying chamber of the drying oven module corresponds to a third layer of left heating oven from bottom to top on the inner side of the material box, and the uppermost drying chamber stays for a period of time in an environment of 55 ℃;

s5, after a period of time, the drying box module continuously and slowly rises along with the single-shaft driver sliding table, the drying box module stops rising when the drying chamber on the uppermost layer of the drying box module corresponds to the left heating box on the topmost layer of the inner side of the material box, the drying chamber on the uppermost layer stays for a period of time in an environment of 50 ℃, and so on, each layer of drying chamber of the drying box module from top to bottom sequentially passes through each layer of left heating box and corresponding right heating box from bottom to top, and stays for a period of time in each layer of left heating box and corresponding right heating box;

s6, when the drying chamber at the lowest layer stays for a period of time in the left heating box at the uppermost layer and the corresponding right heating box, the single-shaft driver sliding table rises to further drive the drying box module to rise, when the drying box module is parallel to the exhaust fan in height, the exhaust fan is started, the exhaust fan air-dries each drying chamber, trehalose crystals begin to appear on the water absorption cloth, and when the water on the water absorption cloth is completely evaporated and the trehalose crystals are not separated out, the single-shaft driver sliding table rises to further drive the drying box module to rise;

s7, when the scraper blade corresponds to the corresponding drying chamber level, the single-shaft driver slip table stops rising, the push rod pushes the fixed block to move rightwards, the fixed block drives each scraper blade fixed rod and the corresponding scraper blade to move rightwards, the plurality of vertically arranged scraper blades simultaneously pass through the plurality of vertically arranged drying chambers, the top end of each scraper blade is in water absorption cloth lower surface contact with the corresponding drying chamber top surface, the bottom surface of each scraper blade is in water absorption cloth upper surface contact with the corresponding drying chamber bottom surface, when the scraper blade moves rightwards, trehalose crystals separated out from the water absorption cloth are scraped off, the trehalose crystals move rightwards along with the scraper blades, and finally fall on the collecting plate, the trehalose on the collecting plate slides down along the collecting plate and falls into the collecting box.

Compared with the prior art, the invention has the beneficial effects that:

in the invention, a left heating box part and a right heating box part are symmetrically fixed on the left side surface and the right side surface in the material box, the left heating box part comprises four left heating boxes which are sequentially fixed on the left side surface in the material box from top to bottom, the right heating box part comprises four right heating boxes, the lowest left heating box is positioned above a feeding pipe, the left outer side wall and the right outer side wall of the material box are respectively provided with an exhaust fan with an air pipe positioned in the material box, the exhaust fan is positioned above the highest heating box, a plurality of drying chambers are arranged in a drying box module from top to bottom, the top surface and the bottom surface in each drying chamber are respectively and fixedly provided with water absorption cloth, the number of the scraping plates and corresponding scraping plate fixing rods is consistent with the number of the drying chambers, the structures and the sizes of the scraping plates are matched with the structures and the sizes of the corresponding drying chambers, the whole crystallization process has no impact on crystals by huge external force, the scraping plates are also used when, the drying box module is provided with a plurality of drying chambers from top to bottom, and the top and bottom surfaces in each drying chamber are fixed with water absorbing cloth, so that crystals can be obtained in the plurality of drying chambers by one-time drying, and the yield is higher;

the process of trehalose crystallization all goes on the cloth that absorbs water, breaks away from the trehalose solution of workbin bottom, and whole device only has the inlet pipe, does not have the discharge gate of other pipeline types to all use the scraper blade to scrape at last and fall in the collecting tray falls at last in, thoroughly avoided traditional crystallization equipment long-term operation to cause the hardening of crystal easily to cause the problem that the discharge gate blocks.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural view of the cabinet module of FIG. 1;

FIG. 3 is a cross-sectional view of the tank of FIG. 1 with the drying tank module submerged in the trehalose solution;

FIG. 4 is a cross-sectional view of the headbox of FIG. 1 with the top tier drying chamber mated with the lowermost heating box;

figure 5 is a cross-sectional view of the headbox of figure 1 with the top tier drying chamber mated with the penultimate tier heating box.

In the drawing, 1, a lifting module, 2, a pushing module, 3, a main body module, 4, a collecting module, 5, a single-shaft driver, 6, a single-shaft driver bracket, 7, a drying box connecting rod, 8, a drying box module, 9, a cylinder, 10, a push rod, 11, a cylinder bracket, 12, a fixed block, 13, a scraper fixing rod, 14, a scraper, 15, a feeding pipe, 16, a fan, 17, a heating box, 18, a heating sheet, 19, a collecting plate, 20, a collecting box, 21, a drying chamber, 22, water absorption cloth, 23, a drying box frame, 24, a bin, 25, a single-shaft driver sliding table, 26, a drying box partition plate, 27, a right heating box, 28, a front support column, 29, a rear support column, 30, a top support column and 31, vertical support columns are arranged.

Detailed Description

Example (b): as shown in fig. 1, 2 and 3, the drying and crystallizing device comprises a lifting module 1, a pushing module 2, a main body module 3 and a collecting module 4; the main body module 3 comprises a feed box 24 with an opening at the upper end, the bottom of the inner side of the feed box 24 is provided with a constant temperature heating sheet 18, the left side wall of the feed box 24 is connected with a feed pipe 15, the left and right side surfaces in the feed box 24 are symmetrically fixed with a left heating box part and a right heating box part, the left heating box part comprises four left heating boxes 17 which are sequentially fixed on the left side surface in the feed box from top to bottom, the right heating box part comprises four left heating boxes 17 at the lowest side of the right heating boxes 27 and positioned above the feed pipe 15, the left and right outer side walls of the feed box 24 are provided with exhaust fans 16 of which air pipes are positioned; the lifting module 1 comprises a single-shaft driver support 6 provided with a vertical single-shaft driver 5, a drying box connecting rod 7 which is vertical and moves up and down under the driving of the single-shaft driver 5 is arranged on the single-shaft driver 5, a drying box module 8 is fixed at the bottom end of the drying box connecting rod 7, the drying box module 8 moves up and down in a material box 24 under the driving of the single-shaft driver 5 along with the drying box connecting rod 7, a plurality of drying chambers 21 are arranged in the drying box module 8 from top to bottom, and water absorbing cloth 22 is fixed on the top and bottom surfaces in each drying chamber 21; the push-out module 2 comprises a cylinder 9 horizontally and transversely arranged, a push rod 10 of the cylinder 9 is rightward, a fixing block 12 is fixedly connected to the right end of the push rod 10, a plurality of horizontally and transversely arranged scraper fixing rods 13 are fixed to the right end of the fixing block 12 from top to bottom, a scraper 14 is fixedly connected to the right end of each scraper fixing rod 13, the number of the scrapers 14 and the number of the corresponding scraper fixing rods 13 are consistent with that of the drying chambers 21, and the structures and the sizes of the scrapers 14 are matched with those of the corresponding drying chambers 21; the right side of the main body module 3 is provided with a collecting module 4, the collecting module 4 comprises a collecting plate 19 for collecting trehalose crystals scraped from a water absorbing cloth 22, and a collecting box 20 is arranged at the lower right side of the collecting plate 19.

The heating temperatures of the four left heating boxes (17) from top to bottom are controlled to 50 ℃, 55 ℃, 60 ℃ and 70 ℃ in sequence, and the heating temperature of the constant temperature heating plate 18 is controlled to 80 ℃.

The single-shaft driver support 6 is vertically arranged on the ground and is a U-shaped frame body with an opening at the left part, the right part and the lower part, the single-shaft driver support 6 comprises a vertical front support 28, a vertical rear support 29 and a horizontal top support 30, the single-shaft driver support 6 is integrally erected at the upper part of the feed box 24, the front support 28 is arranged at the front side of the front side wall of the feed box 24, the rear support 29 is arranged at the rear side of the rear side wall of the feed box 24, a horizontal and longitudinally-arranged cross beam is arranged between the front support 28 and the rear support 29 at the relative position of the upper part of the single-shaft driver support 6, the cross beam is arranged above the feed box 24, a vertical support 31 is arranged between the middle part of the upper surface of the cross beam; install single-axis drive slip table 25 and single-axis drive slip table 25 on single-axis drive 5 and freely remove from top to bottom in 5 stroke range of single-axis drive, the right side of single-axis drive slip table 25 is fixed with the positive center of the 8 top surfaces of the drying cabinet connecting rod 7 and the 7 bottom fixed connection drying cabinet modules of drying cabinet connecting rod 7 of vertical arrangement, drying cabinet connecting rod 7 and all follow single-axis drive slip table 25 with drying cabinet connecting rod 7 fixed connection's drying cabinet module 8 and freely remove from top to bottom.

The drying box module 8 comprises a cuboid drying box frame body 23 which is horizontally arranged and has an opening at the left end and the right end, a plurality of drying box partition plates 26 which are horizontally and transversely arranged are arranged in the drying box frame body 23 from top to bottom, the front end of each drying box partition plate 26 is fixedly connected with the front side surface in the drying box frame body 23, the rear end of each drying box partition plate 26 is fixedly connected with the rear side surface in the drying box frame body 23, the left end and the right end of each drying box partition plate 26 are respectively flush with the left end and the right end of the drying box frame body 23, the drying box partition plates 26 are uniformly distributed along the vertical direction to divide the inside of the drying box into a plurality of drying chambers 21 from top to bottom, the drying chambers 21 have the same structure, water absorbing cloth 22 is respectively and fixedly laid on the top surface and the bottom surface in each drying chamber 21, a certain gap is left between the lower surface of the water absorbing cloth 22 on the top surface of the drying chamber 21, each scraper 14 along with the corresponding scraper fixing rod 13 is driven by the push rod 10 to enter the corresponding drying chamber 21 to scrape the lower surface of the water absorption cloth 22 on the top surface of the corresponding drying chamber 21 and the upper surface of the water absorption cloth 22 on the bottom surface of the corresponding drying chamber 21 simultaneously.

All be equipped with left electric heating wire and left electric heating wire control corresponding left heating box 17 in each left heating box 17 the heating temperature between the left and right heating box part distance and the horizontal transverse distance looks adaptation of drying cabinet framework 23, left heating box 17 is right open-ended rectangle box and opening size, shape and the left and right side opening looks adaptation of drying chamber 21.

The fixed block 12 is vertical to be arranged, the shape of each scraper blade dead lever 13, the structure, the size is all the same, the shape of each scraper blade 14, the structure, the size is all the same, and scraper blade 14 is vertical and arranges along the fore-and-aft direction, each scraper blade 14 top, the bottom respectively with the cloth 22 lower surface that absorbs water of corresponding drying chamber 21 top surface, the cloth 22 upper surface contact that absorbs water of corresponding drying chamber 21 bottom surface, simultaneously each scraper blade 14 is in the free movement about corresponding drying chamber 21, scraper blade dead lever 13 length exceedes the horizontal transverse length of drying chamber 21 and the diameter is less than corresponding drying chamber 21 vertical height, vertical clearance and the vertical thickness looks adaptation of drying cabinet cut-off board 26 between the adjacent scraper blade 14.

The top end of the right side wall of the feed box 24 is fixed with a collecting plate 19 which is obliquely arranged, the left side of the collecting plate 19 is higher and the right side is lower, a collecting box 20 with an upper opening is arranged right below the right end of the collecting plate 19, each scraper 14 enters a corresponding drying chamber 21 to scrape materials when the drying box frame body 23 ascends to the highest position, and the left end of the collecting plate 19 is arranged at the right lower part of the drying box frame body 23 when the collecting plate ascends to the highest position.

The feed box 24 is a rectangular box body horizontally placed on the ground, the heating sheet 18 is arranged at the bottom of the inner side of the feed box 24 and protrudes inwards along the peripheral wall, the size of the space inside the heating sheet 18 is larger than that of the drying box module 8, the heating sheet 18 is a heating sheet formed by an electric heating wire, and the electric heating wire in the heating sheet 18 controls the heating temperature of the heating sheet 18; the structures and the positions of the exhaust fans 16 on the left side wall and the right side wall are symmetrical; the air cylinder 9 is fixedly connected with the feed box 24 through an L-shaped air cylinder bracket 11.

S1, conveying the processed trehalose solution into a material box 24 through a feeding pipe 15, making the liquid level of the trehalose solution lower than the height of the feeding pipe 15, starting a heating sheet 18, controlling the heating temperature of the heating sheet 18 to be 80 ℃, heating the trehalose solution by the heating sheet 18, starting a single-shaft driver 5 to enable a sliding table 25 of the single-shaft driver to descend and drive a connecting rod 7 of a drying box to descend, the connecting rod 7 of the drying box further drives a module 8 of the drying box to descend, when the sliding table 25 of the single-shaft driver descends to a stroke bottom dead center, the module 8 of the drying box completely sinks into the trehalose solution in a heating state, and a water absorption cloth 22 in a drying chamber 21 absorbs the trehalose solution;

s2, after a period of time, the drying box module 8 slowly rises along with the single-shaft driver sliding table 25, the excessive moisture of the water absorption cloth 22 falls back to the trehalose solution at the bottom of the material box 24 again when the single-shaft driver sliding table 25 rises, the drying box module 8 stops rising when the drying box module 8 rises to enable the uppermost layer drying chamber 21 of the drying box module 8 to correspond to the lowest layer left heating box 17 at the inner side of the material box 24, the left heating box 17, the uppermost layer drying chamber 21 and the right heating box 27 form a relatively closed box body together, then all the left heating boxes 17 and the right heating boxes 27 are started, and the heating temperatures of the four left heating boxes from top to bottom are controlled to be 50 ℃, 55 ℃, 60 ℃ and 70 ℃ in sequence, so that the uppermost layer drying chamber 21 stays for a period of time in an environment of 70 ℃;

s3, after a period of time, the drying oven module 8 continuously and slowly rises along with the single-axis driver sliding table 25, so that the drying chamber 21 on the uppermost layer of the drying oven module 8 corresponds to the second layer of left heating boxes 17 on the inner side of the material box 24 from bottom to top, and the drying oven module 8 stops rising when the drying chamber 21 on the second layer of the drying oven module 8 from top to bottom corresponds to the left heating box 17 on the lowermost layer of the inner side of the material box 24, so that the drying chamber 21 on the uppermost layer stays for a period of time in an environment of 60 ℃;

s4, after a period of time, the drying oven module 8 continuously and slowly rises along with the single-shaft driver sliding table 25, so that the drying oven module 8 stops rising when the uppermost drying chamber 21 of the drying oven module 8 corresponds to the third layer of left heating boxes 17 from bottom to top inside the feed box 24, and the uppermost drying chamber 21 stays in an environment of 55 ℃ for a period of time;

s5, after a period of time, the drying box module 8 continuously and slowly rises along with the single-axis driver sliding table 25, so that the drying box module 8 stops rising when the drying chamber 21 on the uppermost layer of the drying box module 8 corresponds to the topmost left heating box 17 inside the material box 24, the drying chamber 21 on the uppermost layer stays in an environment of 50 ℃ for a period of time, and so on, each layer of drying chambers 21 of the drying box module 8 from top to bottom sequentially passes through each layer of left heating box 17 and corresponding right heating box 27 from bottom to top, and stays in each layer of left heating box 17 and corresponding right heating box 27 for a period of time;

s6, after the drying chamber 21 at the lowest layer stays for a period of time in the left heating box 17 at the uppermost layer and the corresponding right heating box 27, the single-shaft driver sliding table 25 rises to further drive the drying box module 8 to rise, when the drying box module 8 is parallel to the exhaust fan 16 in height, the exhaust fan 16 is started, the drying chambers 21 are air-dried by the exhaust fan 16, trehalose crystals begin to appear on the water absorbing cloth 22, and when the moisture on the water absorbing cloth 22 is completely evaporated and the trehalose crystals are not separated out, the single-shaft driver sliding table 25 rises to further drive the drying box module 8 to rise;

s7, when the scraper 14 horizontally corresponds to the corresponding drying chamber 21, the single-shaft driver sliding table 25 stops rising, the push rod 10 pushes the fixed block 12 to move rightwards, the fixed block 12 drives the scraper fixed rods 13 and the corresponding scraper 14 to move rightwards, the plurality of vertically arranged scrapers 14 simultaneously penetrate through the plurality of vertically arranged drying chambers 21, the top ends of the scrapers 14 are in contact with the lower surface of the water absorption cloth 22 on the top surface of the corresponding drying chamber 21, the bottom surfaces of the scrapers 14 are in contact with the upper surface of the water absorption cloth 22 on the bottom surface of the corresponding drying chamber 21, when the scrapers 14 move rightwards, trehalose crystals separated from the water absorption cloth 22 are scraped off, the trehalose crystals move rightwards along with the scrapers 14 and finally fall on the collecting plate 19, and the trehalose on the collecting plate 19 slides down along the.

The preparation of the trehalose solution required by the above equipment can be realized by adopting the existing trehalose preparation method:

firstly, inoculating an enzyme-producing strain into a fermentation culture medium, and culturing for 30-80 h at the temperature of 28-33 ℃ to prepare a fermentation liquid;

freezing and centrifuging the fermentation liquor at 10 ℃ for 1 hour, collecting wet thalli obtained after centrifugation, mixing the wet thalli with a sodium phosphate buffer solution, crushing the thalli by using a high-pressure homogenizer, freezing and centrifuging at 10 ℃ for one hour again, and collecting the obtained supernatant, namely a crude enzyme solution;

adding solid ammonium sulfate powder into the crude enzyme solution, stirring, standing for 3 hr, and collecting supernatant to obtain trehalose synthetase solution;

carrying out cross-linking embedding reaction for 8 hours under the conditions that the mass concentration of glutaraldehyde is 0.5-1.0%, the volume-mass ratio of trehalose synthase solution to chitosan gel is 1: 1-6: 1, the pH value is 8.0, and the temperature is 12-25 ℃, wherein the volume-mass ratio unit is ml/g, so as to prepare immobilized trehalose synthase;

mixing immobilized trehalose synthase with a maltose substrate solution, adding glucoamylase into the mixture, adjusting the pH to 6.0, controlling the saccharification temperature to 65 ℃, carrying out enzymolysis for 8 hours, and filtering the mixture to obtain a trehalose crude solution;

adding activated carbon accounting for 1% of the total mass of the trehalose crude liquid into the trehalose crude liquid for decoloring, stirring and filtering to obtain secondary filtrate;

adding Na 2 CO 3 accounting for 0.6% of the total mass of the secondary filtrate and 70% ethanol accounting for 10% of the total mass of the secondary filtrate, standing for 3 hours, and filtering to obtain a treated trehalose solution.

Claims (10)

1. The utility model provides a high-efficient trehalose extraction equipment, includes dry crystallization device, its characterized in that: the drying and crystallizing device comprises a lifting module (1), a pushing module (2), a main body module (3) and a collecting module (4); the main body module (3) comprises a material box (24) with an opening at the upper end, a constant temperature heating sheet (18) is arranged at the bottom of the inner side of the material box (24), a feeding pipe (15) is connected with the left side wall of the material box (24), left and right heating box parts are symmetrically fixed on the left and right side surfaces in the material box (24), the left heating box part comprises four left heating boxes (17) which are sequentially fixed on the left side surface in the material box from top to bottom, the right heating box part comprises four right heating boxes (27), the left heating box (17) at the lowest side is positioned above the feeding pipe (15), exhaust fans (16) with air pipes positioned in the material box are arranged on the left and right outer side walls of the material box (24), and the exhaust fans (16); the lifting module (1) comprises a single-shaft driver support (6) provided with a vertical single-shaft driver (5), a drying box connecting rod (7) which is vertical and can move up and down under the driving of the single-shaft driver (5) is arranged on the single-shaft driver (5), a drying box module (8) is fixed at the bottom end of the drying box connecting rod (7), the drying box module (8) moves up and down in a material box (24) along with the drying box connecting rod (7) under the driving of the single-shaft driver (5), a plurality of drying chambers (21) are arranged in the drying box module (8) from top to bottom, and water absorbing cloth (22) is fixed on the top and bottom surfaces in each drying chamber (21); the push-out module (2) comprises cylinders (9) which are horizontally and transversely arranged, push rods (10) of the cylinders (9) are rightward, fixing blocks (12) are fixedly connected to the right ends of the push rods (10), a plurality of horizontal and transversely arranged scraper fixing rods (13) are fixed to the right ends of the fixing blocks (12) from top to bottom, scrapers (14) are fixedly connected to the right ends of the scraper fixing rods (13), the quantity of the scrapers (14) and the quantity of the corresponding scraper fixing rods (13) are consistent with that of the drying chambers (21), and the structures and the sizes of the scrapers (14) are matched with those of the corresponding drying chambers (21); the right side of the main body module (3) is provided with a collecting module (4), the collecting module (4) comprises a collecting plate (19) used for collecting trehalose crystals scraped from the water absorbing cloth (22), and a collecting box (20) is arranged at the lower right side of the collecting plate (19).

2. The high-efficiency trehalose extraction equipment according to claim 1, wherein: the heating temperatures of the four left heating boxes (17) from top to bottom are controlled to be 50 ℃, 55 ℃, 60 ℃ and 70 ℃ in sequence, and the heating temperature of the constant temperature heating sheet (18) is controlled to be 80 ℃.

3. The high-efficiency trehalose extraction equipment according to claim 1, wherein: the single-shaft driver support (6) is vertically arranged on the ground and is a left part, a right part, a lower part opened U-shaped frame body, the single-shaft driver support (6) comprises a vertical front support column (28), a rear support column (29) and a horizontal top support column (30), the single-shaft driver support (6) is integrally erected on the upper part of a material box (24), the front support column (28) is arranged on the front side of the front side wall of the material box (24), the rear support column (29) is arranged on the rear side wall of the material box (24), a horizontal longitudinally-arranged cross beam is arranged between the front support column (28) and the rear support column (29) at the relative position of the upper part of the single-shaft driver support (6), the cross beam is positioned above the material box (24), a vertical support column (31) is arranged between the middle part of the upper surface of the cross beam and the middle part of; install unipolar driver slip table (25) and unipolar driver slip table (25) on unipolar driver (5) and freely move from top to bottom in unipolar driver (5) stroke range, unipolar driver slip table (25) right flank is fixed with the positive center of vertical drying cabinet connecting rod (7) and drying cabinet connecting rod (7) bottom fixed connection drying cabinet module (8) top surface of arranging, drying cabinet connecting rod (7) and with drying cabinet connecting rod (7) fixed connection drying cabinet module (8) all follow unipolar driver slip table (25) freely move from top to bottom.

4. The high-efficiency trehalose extraction equipment according to claim 1, wherein: the drying box module (8) comprises a horizontally arranged and left end and a right end open-ended rectangular drying box frame body (23), a plurality of horizontally and transversely arranged drying box partition plates (26) are arranged in the drying box frame body (23) from top to bottom, the front end of each drying box partition plate (26) is fixedly connected with the front side face inside the drying box frame body (23), the rear end of each drying box partition plate (26) is fixedly connected with the rear side face inside the drying box frame body (23), the left end and the right end of each drying box partition plate (26) are respectively flush with the left end and the right end of the drying box frame body (23), the plurality of drying box partition plates (26) are uniformly distributed along the vertical direction to divide the inside of the drying box into a plurality of drying chambers (21) from top to bottom, the plurality of drying chambers (21) are identical in structure, water absorbing cloth (22) is respectively and fixedly laid on the top face and the bottom face inside of each drying chamber (21), and water absorbing cloth (22) on the lower face of the top face of the drying chamber (21) and water absorbing cloth (22) upper And each scraper (14) enters the corresponding drying chamber (21) rightwards along with the corresponding scraper fixing rod (13) under the driving of the push rod (10) so as to scrape the lower surface of the water absorption cloth (22) on the top surface of the corresponding drying chamber (21) and the upper surface of the water absorption cloth (22) on the bottom surface of the corresponding drying chamber (21) simultaneously.

5. The high-efficiency trehalose extraction equipment according to claim 4, wherein: the distance between the left heating box component and the right heating box component of the heating temperature of the left heating box (17) corresponding to the left heating box (17) controlled by the left electric heating wire and the left electric heating wire is matched with the horizontal transverse distance of the drying box frame body (23), and the left heating box (17) is a rectangular box body with an opening at the right side and is matched with the opening size and the opening shape of the left side surface and the right side surface of the drying chamber (21).

6. The high-efficiency trehalose extraction equipment according to claim 1, wherein: the fixed block (12) is arranged vertically, the shape, the structure and the size of each scraper fixing rod (13) are the same, the shape, the structure and the size of each scraper (14) are the same, the scrapers (14) are arranged vertically along the front-back direction, the top and the bottom of each scraper (14) are respectively in contact with the lower surface of the water absorbing cloth (22) on the top surface of the corresponding drying chamber (21) and the upper surface of the water absorbing cloth (22) on the bottom surface of the corresponding drying chamber (21), meanwhile, each scraper (14) freely moves left and right in the corresponding drying chamber (21), the length of each scraper fixing rod (13) exceeds the horizontal transverse length of the drying chamber (21), the diameter of each scraper fixing rod is smaller than the vertical height of the corresponding drying chamber (21), and the vertical gap between the adjacent scrapers (14) is matched with the vertical thickness of the.

7. The high-efficiency trehalose extraction equipment according to claim 4, wherein: the top end of the right side wall of the feed box (24) is fixedly provided with a collecting plate (19) which is obliquely arranged, the left side of the collecting plate (19) is high, the right side of the collecting plate (19) is low, a collecting box (20) with an upper opening is arranged right below the right end of the collecting plate (19), each scraper (14) enters a corresponding drying chamber (21) to scrape materials when the drying box frame body (23) ascends to the highest position, and the left end of the collecting plate (19) is positioned at the right lower part of the drying box frame body (23) when the collecting plate (19) ascends.

8. The high-efficiency trehalose extraction equipment according to claim 1, wherein: the feed box (24) is a rectangular box body horizontally placed on the ground, the heating sheet (18) is arranged at the bottom of the inner side of the feed box (24) and protrudes inwards along the peripheral wall, the size of the space of the inner side of the heating sheet (18) is larger than that of the drying box module (8), the heating sheet (18) is formed by electric heating wires, and the electric heating wires in the heating sheet (18) control the heating temperature of the heating sheet (18); the structures and the positions of the exhaust fans (16) on the left side wall and the right side wall are symmetrical; the air cylinder (9) is fixedly connected with the feed box (24) through an L-shaped air cylinder bracket (11).

9. The extraction method of the high-efficiency trehalose extraction equipment as claimed in any one of the claims 1 to 8, which is characterized by comprising the following steps: firstly, inoculating an enzyme-producing strain into a fermentation culture medium to prepare a fermentation liquid; separating the obtained fermentation liquor by thalli, breaking cell walls and purifying enzyme to obtain trehalose synthase solution; carrying out cross-linking embedding reaction on trehalose synthetase liquid and chitosan gel to prepare immobilized trehalose synthetase; mixing the immobilized trehalose synthetase and a maltose substrate solution, and preparing a trehalose solution through conversion and separation; then, the trehalose solution is dried and crystallized by using the high-efficiency trehalose extraction equipment, and the drying and crystallization steps are as follows:

s1, conveying the processed trehalose solution into a material box (24) through a feeding pipe (15), enabling the liquid level of the trehalose solution to be lower than the height of the feeding pipe (15), starting a heating sheet (18), controlling the heating temperature of the heating sheet (18) to be 80 ℃, heating the trehalose solution by the heating sheet (18), starting a single-shaft driver (5) to enable a single-shaft driver sliding table (25) to descend and drive a drying box connecting rod (7) to descend, driving the drying box module (8) to descend by the drying box connecting rod (7), completely sinking the drying box module (8) into the trehalose solution in a heating state when the single-shaft driver sliding table (25) descends to a stroke bottom dead center, and absorbing the trehalose solution by a water absorbing cloth (22) in a drying chamber (21);

s2, after a period of time, the drying box module (8) slowly rises along with the single-shaft driver sliding table (25), redundant moisture of the water absorption cloth (22) falls back to the trehalose solution at the bottom of the material box (24) again when the single-shaft driver sliding table (25) rises, the drying box module (8) stops rising when the uppermost layer drying chamber (21) of the drying box module (8) corresponds to the lowest layer left heating box (17) in the material box (24), the left heating box (17), the uppermost layer drying chamber (21) and the right heating box (27) form a relatively closed box body together, then all the left heating box (17) and the right heating box (27) are started, and the heating temperatures of the four left heating boxes from top to bottom are controlled to be 50 ℃, 55 ℃, 60 ℃ and 70 ℃ in sequence, and the uppermost layer drying chamber (21) is stopped in a 70 ℃ environment for a period of time;

s3, after a period of time, the drying box module (8) continuously and slowly rises along with the single-shaft driver sliding table (25), so that the uppermost layer of drying room (21) of the drying box module (8) corresponds to the second layer of left heating boxes (17) counting from bottom to top on the inner side of the material box (24), and meanwhile, when the second layer of drying room (21) of the drying box module (8) corresponds to the lowest layer of left heating boxes (17) on the inner side of the material box (24) from top to bottom, the drying box module (8) stops rising, so that the uppermost layer of drying room (21) stays for a period of time in an environment at 60 ℃;

s4, after a period of time, the drying box module (8) continuously and slowly rises along with the single-shaft driver sliding table (25), so that the drying box module (8) stops rising when the uppermost layer of drying chambers (21) of the drying box module (8) correspond to the third layer of left heating boxes (17) which are arranged on the inner side of the material box (24) from bottom to top, and the uppermost layer of drying chambers (21) stay for a period of time in an environment of 55 ℃;

s5, after a period of time, the drying box module (8) continuously and slowly rises along with the single-shaft driver sliding table (25), the drying box module (8) stops rising when the uppermost layer drying chamber (21) of the drying box module (8) corresponds to the topmost layer left heating box (17) on the inner side of the material box (24), the uppermost layer drying chamber (21) is kept for a period of time in an environment with the temperature of 50 ℃, and so on, each layer of drying chamber (21) of the drying box module (8) from top to bottom sequentially passes through each layer of left heating box (17) and corresponding right heating box (27), and each layer of left heating box (17) and corresponding right heating box (27) are kept for a period of time;

s6, when the lowermost drying chamber (21) stays for a period of time in the uppermost left heating box (17) and the corresponding right heating box (27), the single-shaft driver sliding table (25) rises to further drive the drying box module (8) to rise, when the drying box module (8) is highly parallel to the exhaust fan (16), the exhaust fan (16) is started, the exhaust fan (16) air-dries each drying chamber (21), trehalose crystals begin to appear on the water absorption cloth (22), and when the moisture on the water absorption cloth (22) is completely evaporated and the trehalose crystals are not separated out, the single-shaft driver sliding table (25) rises to further drive the drying box module (8) to rise;

s7, when the scrapers (14) horizontally correspond to the corresponding drying chambers (21), the single-shaft driver sliding table (25) stops rising, the push rod (10) pushes the fixing block (12) to move rightwards, the fixing block (12) drives each scraper fixing rod (13) and the corresponding scraper (14) to move rightwards, the plurality of vertically arranged scrapers (14) simultaneously penetrate through the plurality of vertically arranged drying chambers (21), the top ends of the scrapers (14) are in contact with the lower surfaces of the water absorbing cloth (22) on the top surfaces of the corresponding drying chambers (21), the bottom surfaces of the scrapers (14) are in contact with the upper surfaces of the water absorbing cloth (22) on the bottom surfaces of the corresponding drying chambers (21), when the scrapers (14) move rightwards, trehalose crystals separated from the water absorbing cloth (22) are scraped off, the trehalose crystals move rightwards along with the scrapers (14), finally fall off the collecting plate (19), and the trehalose on the collecting plate (19) slides down along the collecting, falls into the collection box (20).

10. The extraction method of the high-efficiency trehalose extraction equipment according to claim 9, wherein the preparation of the trehalose solution comprises the following specific steps:

firstly, inoculating an enzyme-producing strain into a fermentation culture medium, and culturing for 30-80 h at the temperature of 28-33 ℃ to prepare a fermentation liquid;

freezing and centrifuging the fermentation liquor at 10 ℃ for 1 hour, collecting wet thalli obtained after centrifugation, mixing the wet thalli with a sodium phosphate buffer solution, crushing the thalli by using a high-pressure homogenizer, freezing and centrifuging at 10 ℃ for one hour again, and collecting the obtained supernatant, namely a crude enzyme solution;

adding solid ammonium sulfate powder into the crude enzyme solution, stirring, standing for 3 hr, and collecting supernatant to obtain trehalose synthetase solution;

carrying out cross-linking embedding reaction for 8 hours under the conditions that the mass concentration of glutaraldehyde is 0.5-1.0%, the volume-mass ratio of trehalose synthase solution to chitosan gel is 1: 1-6: 1, the pH value is 8.0, and the temperature is 12-25 ℃, wherein the volume-mass ratio unit is ml/g, so as to prepare immobilized trehalose synthase;

mixing immobilized trehalose synthase with a maltose substrate solution, adding glucoamylase into the mixture, adjusting the pH to 6.0, controlling the saccharification temperature to 65 ℃, carrying out enzymolysis for 8 hours, and filtering the mixture to obtain a trehalose crude solution;

adding activated carbon accounting for 1% of the total mass of the trehalose crude liquid into the trehalose crude liquid for decoloring, stirring and filtering to obtain secondary filtrate;

adding Na 2 CO 3 accounting for 0.6% of the total mass of the secondary filtrate and 70% ethanol accounting for 10% of the total mass of the secondary filtrate, standing for 3 hours, and filtering to obtain a treated trehalose solution.

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