CN110251984A - A kind of efficient trehalose extract equipment and its extracting method - Google Patents

Abstract

The invention discloses a kind of efficient trehalose extract equipment and its extracting methods, including going up and down module, release module, main body module and collection module, main body module includes the hopper of upper end opening, hopper inside bottom is equipped with heated at constant temperature piece, hopper left side wall is connected with feed pipe, it is left inside hopper, right side is symmetrically fixed with a left side, right heating box part, hopper is left, right outside sidewall is designed with the exhaust fan that air hose is in hopper, lifting module includes the uniaxial driver rack for being equipped with vertical uniaxial driver, release the cylinder that module includes horizontal cross arrangement, collection module includes the collecting board for collecting the scraped seaweed sugar crystal from absorbent cloth, the invention has the benefit that can not only high efficiency extraction go out seaweed sugar crystal and be also avoided that the problem of traditional extract equipment longtime running leads to Crystallization Plugging discharge port.

Description

A kind of efficient trehalose extract equipment and its extracting method

Technical field

The invention belongs to the technical fields that trehalose extracts, and in particular to a kind of efficient trehalose extract equipment and its extraction Method.

Background technique

Trehalose is irreducibility disaccharide made of being closed by two glucose molecules by the bond of α, α -1,1, is widely present In bacterium, fungi, algae, rudimentary plant and insect.It has been investigated that the sugar has unique biological function, there is protection Large biological molecule, protect cell membrane, protected protein matter from freezing, drying and osmotic pressure variation etc. caused by destruction effect, Extensive use is all had in fields such as food, medicine, cosmetics, agriculturals.

Currently, the preparation method comprises the following steps: bacterium producing multi enzyme preparation is inoculated in fermentation medium, fermentation liquid is made in existing trehalose, By fermentation liquid through thallus separation, thallus broken wall, enzyme purification, trehalose synthetase liquid is made, trehalose synthetase liquid is handed over Connection embedding reaction, is made immobilization trehalose synthetase, immobilization trehalose synthetase is mixed with maltose substrate solution, pass through Conversion, separation crystallize obtained trehalose, the preparation method of fermentation liquid, trehalose synthetase liquid, immobilization trehalose synthetase etc. It is all the prior art.

It include enzyme bacterium culture device, enzyme bacterium Zymolysis Equipment, thallus broken wall equipment, Synthesis liquid centrifugation in trehalose extract equipment Equipment, mixed solution separating and filtering equipment, drying crystalline is mixed in equipment, crosslinking embedding consersion unit, synthesis enzyme-to-substrate Device.

But the drying crystalline device of each company's trehalose is different, that there are contents is unstable, crystal form is irregular, mobility The problem of difference, agglomeration, purity difference, crystallization time length etc., moreover, crystal block section is with reaction when traditional crystallization apparatus work It constantly is deposited in crystallizer bottom, longtime running be easy to cause the hardened of crystal, causes discharging very difficult or even blocks out Material mouth needs regular stop cleaning or reduces reaction density to develop the crystallization of discharge port, causes crystalline rate low, because This, is badly in need of finding the novel trehalose extract equipment of one kind and extracting method solves problem above.

Summary of the invention

The present invention provides a kind of efficient trehalose extract equipment and its extracting method, can not only high efficiency extraction go out trehalose crystalline substance Body is also avoided that the problem of traditional extract equipment longtime running leads to Crystallization Plugging discharge port.

In order to solve the above-mentioned technical problem, the present invention adopts the following technical scheme:

A kind of trehalose extract equipment, including drying crystalline device, drying crystalline device include lifting module, release module, master Module and collection module；Main body module includes the hopper of upper end opening, and hopper inside bottom is equipped with heated at constant temperature piece, and hopper is left Side wall is connected with feed pipe, and left and right side is symmetrically fixed with left and right heating box part inside hopper, Zuo Jiare box part include from Top to bottm is in turn secured in hopper four left heater boxes on left side, and right heating box part includes that four right heater boxes are most lower The left heater box in side is in above feed pipe, and the left and right lateral wall of hopper is designed with the exhaust fan that air hose is in hopper, at exhaust fan Above the heater box of top side；Lifting module includes the uniaxial driver rack for being equipped with vertical uniaxial driver, single shaft driving Drying box connecting rod that is vertical and moving up and down under the driving of uniaxial driver is installed, drying box connecting rod bottom end is fixed on device Dry tank module, dry tank module moves up and down in hopper under the driving of uniaxial driver in company with drying box connecting rod, dry Top, bottom surface inside multiple hothouses and each hothouse are equipped in tank module from top to bottom and is all fixed with absorbent cloth；Release module packet Include the cylinder of horizontal cross arrangement, the push rod of cylinder is to the right and push rod right end is fixed with fixed block, fixed block right end on to The scraper plate fixed link of more horizontal cross of lower fixation arrangement, each scraper plate fixed link right end are fixed with scraper plate, scraper plate and correspondence The quantity of scraper plate fixed link is consistent with hothouse quantity, and the structure and size of scraper plate and the structure and size of corresponding hothouse are mutually fitted Match；Collection module is equipped on the right side of main body module, collection module includes for collecting the scraped seaweed sugar crystal from absorbent cloth Collecting board, collecting board lower right are equipped with collecting box.

Further, the heating temperature of four left heater boxes from top to bottom successively controls as 50 DEG C, 55 DEG C, 60 DEG C, 70 DEG C, and the heating and temperature control of heated at constant temperature piece is 80 DEG C.

Further, uniaxial driver rack be arranged vertically on ground and in left part, right part, lower openings U-typed Framework, uniaxial driver rack include the topmast column of vertical front standing pillar and back prop and level, and uniaxial driver rack is whole Body is set up in hopper top, and front standing pillar is set on front side of hopper front side wall, and back prop is set on rear side of hopper rear wall, uniaxial driver Frame upper relative position is equipped with horizontal longitudinally arranged crossbeam between front standing pillar and back prop, and crossbeam is in above hopper, It is equipped with vertical support column between in the middle part of beam surface upper and in the middle part of topmast column lower surface, vertical support column right side is fixed with vertically The uniaxial driver of arrangement；Uniaxial driver slide unit and uniaxial driver slide unit are installed in uniaxial driver on uniaxial driver It is freely up and down moved in stroke range, uniaxial driver slide unit right side is fixed with the drying box connecting rod arranged vertically and drying Case connecting rod bottom end is fixedly connected with the center of dry tank module top surface, drying box connecting rod and with drying box connecting rod is fixed connects The drying tank module connect follows uniaxial driver slide unit freely up and down to move.

Further, dry tank module includes horizontally disposed and the dry cassette frame of the cuboid of left end, right-end openings, is done Be equipped with the drying box reticle of multiple horizontal cross arrangement in dry cassette frame from top to bottom, each drying box segmentation front edge of board with it is dry Dry cassette frame inner front side face is fixedly connected, and drying box reticle rear end is fixedly connected with dry cassette frame inner back side face, Each left and right end of drying box reticle is flushed with the dry left and right end of cassette frame respectively, and multiple drying box reticles are equal along the vertical direction Even distribution will be divided into from top to bottom multiple hothouses inside drying box, multiple drying chamber structures are identical and each hothouse inside Top surface and bottom surface are fixed respectively is equipped with absorbent cloth, on the absorbent cloth lower surface of hothouse top surface and the absorbent cloth of hothouse bottom surface There are certain interval between surface, each scraper plate enters to the right corresponding hothouse under push rod drive in company with corresponding scraper plate fixed link It is interior, with to corresponding hothouse top surface absorbent cloth lower surface and the absorbent cloth upper surface of corresponding hothouse bottom surface scrape simultaneously Material.

Further, it is equipped with left electric heating wire in each left heater box and left electric heating wire controls adding for corresponding left heater box The distance between hot left and right heating box part of temperature and dry cassette frame horizontal cross distance are adapted, and left heater box is right side Rectangular box and openings of sizes, the shape of opening are adapted with hothouse left and right side opening.

Further, fixed block is vertical arrangement, and shape, construction, the size of each scraper plate fixed link are all the same, each scraper plate Shape, construction, size are all the same, and scraper plate is vertical and arranges along the longitudinal direction, each scraper plate top and bottom ends respectively with corresponding hothouse The absorbent cloth upper surface contact of the absorbent cloth lower surface of top surface, corresponding hothouse bottom surface, while each scraper plate is in corresponding hothouse Left and right moves freely, and the fixed pole length of scraper plate is more than hothouse horizontal cross length and diameter is high vertically less than corresponding hothouse It spends, the vertical gap between adjacent scraper plate is adapted with the vertical thickness of drying box reticle.

Further, hopper right side wall top is fixed with the collecting board of inclination arrangement, and high right side is low on the left of collecting board, collects Plate right end is arranged right below the collecting box of upper opening, and dry cassette frame rises to each scraper plate when highest position and enters corresponding do Dry indoor with scraper, collecting board left end is at the lower right of drying cassette frame when rising to highest position.

Further, hopper is the rectangular box for being placed horizontally at ground, and heating sheet was set to hopper inside bottom and along week Wall is inwardly projecting, and heating sheet inner space size is greater than drying box module size, and heating sheet is the heating sheet that electric heating wire is formed, The heating temperature of electric heating wire control heating sheet in heating sheet；The structure of exhaust fan, position are symmetrical in left and right side walls； Cylinder is fixedly connected with hopper by " L " type air cylinder support.

Further, the extracting method of above-mentioned a kind of efficient trehalose extract equipment, it is characterised in that: first by bacterium producing multi enzyme preparation It is inoculated in fermentation medium, fermentation liquid is made；By fermentation liquid obtained through thallus separation, thallus broken wall, enzyme purification, sea is made Algae sugar synthesizes enzyme solution；Trehalose synthetase liquid is carried out the obtained immobilization trehalose of crosslinking embedding reaction with chitosan gel rubber to synthesize Enzyme；Immobilization trehalose synthetase is mixed with maltose substrate solution, aqueous trehalose is made in inverted, separation；Then make With a kind of above-mentioned efficient trehalose extract equipment by aqueous trehalose drying crystalline, the step of drying crystalline, is as follows:

S1, the aqueous trehalose handled well is transported in hopper by feed pipe, allows aqueous trehalose liquid level lower than feed pipe Highly, starting heating sheet and controlling the heating temperature of heating sheet is 80 DEG C, and aqueous trehalose is heated piece heating, and starts single shaft For driver so that uniaxial driver slide unit declines and drying box connecting rod is driven to decline, drying box connecting rod drives dry tank module in turn Decline, when uniaxial driver slide unit drops to bottom of stroke, dry tank module sinks to the trehalose in heated condition completely In solution, dry indoor absorbent cloth absorbs aqueous trehalose；

S2, it after a period of time, dries tank module and slowly rises in company with uniaxial driver slide unit, inhaled in uniaxial driver slide unit rising The extra moisture of water cloth is dropped back into again in the aqueous trehalose of hopper bottom, and dry tank module, which rises to, keeps dry tank module most upper On the inside of layer hothouse and hopper the left heater box of lowest level to it is corresponding when module stop rising, left heater box, top layer's drying Room and right heater box surround the cabinet of relative closure together, then start all left heater boxes, right heater box, and make from top to bottom The heating temperatures of four left heater boxes successively control and allow top layer's hothouse in 70 DEG C of environment for 50 DEG C, 55 DEG C, 60 DEG C, 70 DEG C In stay for some time；

S3, after a period of time, dry tank module continues slowly to rise in company with uniaxial driver slide unit, makes dry tank module top layer It is corresponding that the left heater boxes of the second layer are counted on the inside of hothouse and hopper from bottom to top, while making dry tank module the second layer is done from top to bottom On the inside of dry room and hopper the left heater box of lowest level to it is corresponding when module stop rising, allow top layer's hothouse in 60 DEG C of environment In stay for some time；

S4, after a period of time, dry tank module continues slowly to rise in company with uniaxial driver slide unit, makes dry tank module top layer Counted from bottom to top on the inside of hothouse and hopper the left heater boxes of third layer to it is corresponding when module stop rising, allow top layer's drying Room stays for some time in 55 DEG C of environment；

S5, after a period of time, dry tank module continues slowly to rise in company with uniaxial driver slide unit, makes dry tank module top layer On the inside of hothouse and hopper the left heater box of top to it is corresponding when module stop rising, allow top layer's hothouse in 50 DEG C of rings It stays for some time in border, and so on, each layer of hothouse of dry tank module from top to bottom all successively passes through from top to bottom It the left heater box of each layer, corresponding right heater box and all stays for some time in every layer of left heater box, corresponding right heater box；

S6, when lowest level hothouse is after the left heater box of top layer, corresponding right heater box stay for some time, uniaxial driver is sliding Platform rising is further driven to dry tank module and rises, and stops rising when dry tank module is parallel with exhaust fan height, starting is taken out Blower, exhaust fan carry out each hothouse air-dried, start trehalose crystallization occur on absorbent cloth, complete to the moisture on absorbent cloth Evaporation and seaweed sugar crystal is when being no longer precipitated, uniaxial driver slide unit rising is further driven to dry tank module and rises；

S7, when scraper plate and corresponding hothouse level to it is corresponding when driver slide unit stop rising, push rod pushes fixed block to the right Movement, fixed block drive each scraper plate fixed link and corresponding scraper plate to move right, and the multiple scraper plates being vertically arranged are also cross vertical Multiple hothouses of arrangement, scraper plate top and corresponding hothouse top surface absorbent cloth following table face contact, scraper plate bottom surface and corresponding drying The contact of room bottom surface absorbent cloth upper surface while scraper plate moves right, the trehalose being precipitated on absorbent cloth crystallization is scraped, seaweed Sugar crystallization follows scraper plate to move right together, finally falls on collecting board, the trehalose on collecting board is slided along collecting board, is fallen It falls in collecting box.

Compared with the existing technology, the invention has the benefit that

In the present invention, left and right side is symmetrically fixed with left and right heating box part inside hopper, and Zuo Jiare box part includes from upper Four left heater boxes being in turn secured in hopper under on left side, right heating box part include four right heater box lower sides Left heater box is in above feed pipe, and the left and right lateral wall of hopper is designed with the exhaust fan that air hose is in hopper, and exhaust fan is in It is all solid that top, bottom surface inside multiple hothouses and each hothouse are equipped with above the heater box of top side, in dry tank module from top to bottom Surely have an absorbent cloth, the quantity of scraper plate and corresponding scraper plate fixed link is consistent with hothouse quantity, and the structure and size of scraper plate with accordingly The structure and size of hothouse are adapted, and entire crystallization process does not have impact of the huge external force to crystal, are also only used when collecting crystal Scraper plate scrapes, the inviolent temperature change of crystallizing system, and environment locating for crystalline particle does not have excessive concentration difference, without crystalline substance Hardened caused by the bulk deposition of body, the crystal size size of output is full, pattern is complete, epigranular, and drying box mould Be equipped with top inside multiple hothouses and each hothouse from top to bottom in block, bottom surface is all fixed with absorbent cloth, primary drying can be Multiple hothouses obtain crystal, and output is more efficient；

Trehalose crystallization process carried out all on absorbent cloth, be detached from hopper bottom aqueous trehalose, whole device only into Expects pipe without the discharge port of other pipeline types, and finally all scrapes collecting board using scraper plate and finally falls in collecting box, Thoroughly avoid hardened the problem of causing discharge port to block that traditional crystallizer longtime running be easy to cause crystal.

Detailed description of the invention

Fig. 1 is the structural diagram of the present invention；

Fig. 2 is the structural schematic diagram of dry tank module in Fig. 1；

Fig. 3 is the cross-sectional view of hopper in Fig. 1 when dry tank module is immersed in aqueous trehalose；

Fig. 4 is the cross-sectional view of hopper in Fig. 1 when top layer hothouse and lowest level heater box cooperate；

Fig. 5 is the cross-sectional view of hopper in Fig. 1 when top layer hothouse and layer heater box second from the bottom cooperate.

In figure, 1. lifting modules, 2. release module, and 3. main body modules, 4. collection modules, 5. uniaxial drivers, 6. is uniaxial Driver rack, 7. drying box connecting rods, 8. dry tank modules, 9. cylinders, 10. push rods, 11. air cylinder supports, 12. fixed blocks, 13. scraper plate fixed link, 14. scraper plates, 15. feed pipes, 16. blowers, 17. heater boxes, 18. heating sheets, 19. collecting boards, 20. collect Case, 21. hothouses, 22. absorbent cloths, 23. dry cassette frames, 24. hoppers, 25. uniaxial driver slide units, the segmentation of 26. drying boxes Plate, 27. right heater boxes, 28. front standing pillars, 29. back props, 30. topmast columns, 31. vertical support columns.

Specific embodiment

Embodiment: as shown in Figure 1, Figure 2, Figure 3 shows, drying crystalline device includes lifting module 1, releases module 2, main body module 3 and collection module 4；Main body module 3 includes the hopper 24 of upper end opening, and 24 inside bottom of hopper is equipped with heated at constant temperature piece 18, material 24 left side wall of case is connected with feed pipe 15, and 24 inside left and right side of hopper is symmetrically fixed with left and right heating box part, left heater box Component includes four left heater boxes 17 being in turn secured in hopper from top to bottom on left side, and right heating box part includes four The right left heater box 17 in 27 lower side of heater box is in 15 top of feed pipe, and the left and right lateral wall of hopper 24 is designed with air hose and is in material Exhaust fan 16 in case, exhaust fan 16 are in 17 top of top side heater box；Lifting module 1 includes being equipped with vertical uniaxial driving The uniaxial driver rack 6 of device 5 is equipped on uniaxial driver 5 vertical and moving up and down under the driving of uniaxial driver 5 dry Dry case connecting rod 7, the fixed dry tank module 8 in 7 bottom end of drying box connecting rod, dry tank module 8 is in company with drying box connecting rod 7 in list Axis driver 5 moves up and down in hopper 24 under driving, and is equipped with multiple hothouses 21 and each dry in dry tank module 8 from top to bottom Top, bottom surface are all fixed with absorbent cloth 22 inside dry room 21；The cylinder 9 that module 2 includes horizontal cross arrangement is released, cylinder 9 pushes away Bar 10 is to the right and 10 right end of push rod is fixed with fixed block 12, and 12 right end of fixed block fixes more horizontal cross arrangements from top to bottom Scraper plate fixed link 13, each 13 right end of scraper plate fixed link is fixed with scraper plate 14, scraper plate 14 and corresponding scraper plate fixed link 13 Quantity is consistent with 21 quantity of hothouse, and the structure and size of scraper plate 14 are adapted with the structure and size of corresponding hothouse 21；It is main Collection module 4 is equipped on the right side of module 3, collection module 4 includes for collecting the scraped seaweed sugar crystal from absorbent cloth 22 Collecting board 19,19 lower right of collecting board are equipped with collecting box 20.

The heating temperature of the left heater box (17) of four from top to bottom successively controls as 50 DEG C, 55 DEG C, 60 DEG C, 70 DEG C, and The heating and temperature control of heated at constant temperature piece 18 is 80 DEG C.

Uniaxial driver rack 6 be arranged vertically on ground and in left part, right part, lower openings U-typed framework, it is uniaxial Driver rack 6 includes the topmast column 30 of vertical front standing pillar 28 and back prop 29 and level, and uniaxial driver rack 6 is whole It is set up in 24 top of hopper, front standing pillar 28 is set on front side of 24 front side wall of hopper, and back prop 29 is set on rear side of 24 rear wall of hopper, single 6 top relative position of axis driver rack is equipped with horizontal longitudinally arranged crossbeam, crossbeam between front standing pillar 28 and back prop 29 Above hopper 24, it is equipped with vertical support column 31 between the middle part of 30 lower surface of topmast column in the middle part of beam surface upper, it is vertical to prop up 31 right side of dagger is fixed with the uniaxial driver 5 arranged vertically；Be equipped on uniaxial driver 5 uniaxial driver slide unit 25 and Uniaxial driver slide unit 25 freely up and down moves in uniaxial 5 stroke range of driver, and uniaxial 25 right side of driver slide unit is solid Surely there is the drying box connecting rod 7 arranged vertically and 7 bottom end of drying box connecting rod is fixedly connected with the center of dry 8 top surface of tank module The heart, drying box connecting rod 7 and the drying tank module 8 being fixedly connected with drying box connecting rod 7 follow uniaxial driver slide unit 25 Freely up and down move.

Dry tank module 8 includes the dry cassette frame 23 of cuboid of horizontally disposed and left end, right-end openings, dry box-frame Be equipped with the drying box reticle 26 of multiple horizontal cross arrangement in body 23 from top to bottom, each 26 front end of drying box reticle with it is dry Dry 23 inner front side face of cassette frame is fixedly connected, and 26 rear end of drying box reticle is fixed with dry 23 inner back side face of cassette frame Connection, the left and right end of each drying box reticle 26 are flushed with the dry left and right end of cassette frame 23 respectively, multiple drying box reticles 26 It is uniformly distributed along the vertical direction multiple hothouses 21 will be divided into from top to bottom inside drying box, multiple 21 structures of hothouse are identical And fixation is equipped with absorbent cloth 22,22 following table of absorbent cloth of 21 top surface of hothouse respectively for the top surface inside each hothouse 21 and bottom surface There are certain intervals between 22 upper surface of absorbent cloth of 21 bottom surface of face and hothouse, and each scraper plate 14 is in company with corresponding scraper plate fixed link 13 Push rod 10 drive under to the right enter corresponding hothouse 21 in, with to corresponding 21 top surface of hothouse 22 lower surface of absorbent cloth and phase It answers 22 upper surface of absorbent cloth of 21 bottom surface of hothouse while carrying out scraper.

It is equipped with left electric heating wire in each left heater box 17 and left electric heating wire controls the heating temperature of accordingly left heater box 17 It spends the distance between left and right heating box part and dry 23 horizontal cross distance of cassette frame is adapted, left heater box 17 is right side Rectangular box and openings of sizes, the shape of opening are adapted with 21 left and right side of hothouse opening.

Fixed block 12 is vertical arrangement, and shape, construction, the size of each scraper plate fixed link 13 are all the same, the shape of each scraper plate 14 Shape, construction, size are all the same, and scraper plate 14 is vertical and arranges along the longitudinal direction, and each 14 top and bottom ends of scraper plate are dried with corresponding respectively 22 upper surface of the absorbent cloth contact of 22 lower surface of absorbent cloth, 21 bottom surface of corresponding hothouse of 21 top surface of room, while each scraper plate 14 exists Left and right moves freely in corresponding hothouse 21, and 13 length of scraper plate fixed link is more than 21 horizontal cross length of hothouse and diameter is less than Corresponding hothouse 21 is vertical highly, and the vertical thickness in vertical gap and drying box reticle 26 between adjacent scraper plate 14 is mutually fitted Match.

24 right side wall top of hopper is fixed with the collecting board 19 of inclination arrangement, 19 low, the collecting board in the high right side in left side of collecting board 19 right ends are arranged right below the collecting box 20 of upper opening, and dry cassette frame 23 rises to each scraper plate 14 when highest position and enters With scraper in corresponding hothouse 21,19 left end of collecting board is in the lower right of the drying cassette frame 23 when rising to highest position Place.

Hopper 24 is the rectangular box for being placed horizontally at ground, heating sheet 18 be set to 24 inside bottom of hopper and along peripheral wall to Interior protrusion, 18 inner space size of heating sheet are greater than dry 8 size of tank module, and heating sheet 18 is the heating that electric heating wire is formed Piece, the heating temperature of the electric heating wire control heating sheet 18 in heating sheet 18；The structure of exhaust fan 16, position in left and right side walls Symmetrically；Cylinder 9 is fixedly connected with hopper 24 by " L " type air cylinder support 11.

A kind of extracting method of above-mentioned efficient trehalose extract equipment, S1, passes through charging for the aqueous trehalose handled well Pipe 15 is transported in hopper 24, is allowed aqueous trehalose liquid level lower than 15 height of feed pipe, is started heating sheet 18 and control heating sheet 18 heating temperature is 80 DEG C, and aqueous trehalose is heated the heating of piece 18, and starts uniaxial driver 5 so that uniaxial driver is sliding Platform 25 declines and drying box connecting rod 7 is driven to decline, and drying box connecting rod 7 drives dry tank module 8 to decline in turn, and uniaxial driver is sliding When platform 25 drops to bottom of stroke, dry tank module 8 is sunk to completely in the aqueous trehalose in heated condition, hothouse 21 Interior absorbent cloth 22 absorbs aqueous trehalose；

S2, it after a period of time, dries tank module 8 and slowly rises in company with uniaxial driver slide unit 25, on uniaxial driver slide unit 25 The extra moisture of absorbent cloth 22 is dropped back into again in the aqueous trehalose of 24 bottom of hopper in liter, and dry tank module 8, which rises to, to be made to do Module 8 stops rising when dry 8 top layer's hothouse 21 of tank module is corresponding with hopper left 17 pairs of the heater box of 24 inside lowest level, Left heater box 17, top layer's hothouse 21 and right heater box 27 surround the cabinet of relative closure together, then start all left sides and add Hot tank 17, right heater box 27, and control the heating temperature of four left heater boxes from top to bottom successively for 50 DEG C, 55 DEG C, 60 DEG C, 70 DEG C allow top layer's hothouse 21 to stay for some time in 70 DEG C of environment；

S3, it after a period of time, dries tank module 8 and continues slowly to rise in company with uniaxial driver slide unit 25, make dry tank module 8 most It is corresponding that 24 inside of upper layer hothouse 21 and hopper counts the left heater boxes 17 of the second layer from bottom to top, while making to dry tank module 8 from upper Module 8 stops rising when corresponding with hopper left 17 pairs of the heater box of 24 inside lowest level to lower second layer hothouse 21, allows most Upper layer hothouse 21 stays for some time in 60 DEG C of environment；

S4, it after a period of time, dries tank module 8 and continues slowly to rise in company with uniaxial driver slide unit 25, make dry tank module 8 most Module 8 stops rising when upper layer hothouse 21 is corresponding with left 17 pairs of the heater box of third layer is counted on the inside of hopper 24 from bottom to top, Top layer's hothouse 21 is allowed to stay for some time in 55 DEG C of environment；

S5, it after a period of time, dries tank module 8 and continues slowly to rise in company with uniaxial driver slide unit 25, make dry tank module 8 most Module 8 stops rising when upper layer hothouse 21 is corresponding with hopper left 17 pairs of the heater box of 24 inside top, makes top layer dry Dry room 21 stays for some time in 50 DEG C of environment, and so on, each layer of hothouse 21 of dry tank module 8 from top to bottom is all From top to bottom successively by the left heater box 17 of each layer, corresponding right heater box 27 and in every layer of left heater box 17, corresponding right heating Case 27 all stays for some time；

S6, when lowest level hothouse 21 is after the left heater box 17 of top layer, corresponding right heater box 27 stay for some time, single shaft drives The dynamic rising of device slide unit 25 is further driven to dry tank module 8 and rises, and stops when dry tank module 8 is parallel with 16 height of exhaust fan Rising, starts exhaust fan 16, exhaust fan 16 carries out each hothouse 21 air-dried, start trehalose crystallization occur on absorbent cloth 22, When the moisture evaporating completely on absorbent cloth 22 and when seaweed sugar crystal is no longer precipitated, uniaxial driver slide unit 25 rises further band Dynamic dry tank module 8 rises；

S7, when scraper plate 14 and corresponding hothouse 21 it is horizontal to it is corresponding when driver slide unit 25 stop rising, push rod 10 pushes admittedly Determine block 12 to move right, fixed block 12 drives each scraper plate fixed link 13 and corresponding scraper plate 14 to move right, and what is be vertically arranged is multiple Scraper plate 14 is also cross the multiple hothouses 21 being vertically arranged, 14 top of scraper plate and corresponding 21 top surface absorbent cloth of hothouse, 22 following table Face contact, 14 bottom surface of scraper plate is contacted with corresponding 21 bottom surface absorbent cloth of hothouse, 22 upper surface, will while scraper plate 14 moves right The trehalose crystallization being precipitated on absorbent cloth 22 scrapes, and trehalose crystallization follows scraper plate 14 to move right together, finally falls and is receiving Collect on plate 19, the trehalose on collecting board 19 is slided along collecting board 19, is fallen in collecting box 20.

The preparation of aqueous trehalose needed for above equipment can be realized using existing trehalose preparation method:

First bacterium producing multi enzyme preparation is inoculated in fermentation medium, under conditions of 28 DEG C ~ 33 DEG C of temperature, 30h ~ 80h is cultivated, hair is made Zymotic fluid；

Fermentation liquid is carried out refrigerated centrifuge 1 hour under the conditions of 10 DEG C, the wet thallus obtained after centrifugation is collected, by wet thallus and phosphorus Sour sodium buffer mixing, then high pressure homogenizer is used, the broken of thallus is carried out, carries out the refrigerated centrifuge one under the conditions of 10 DEG C again Hour, collecting resulting supernatant is crude enzyme liquid；

Solid ammonium sulfate powder is added in crude enzyme liquid, 3 hours is stood after stirring, takes supernatant, trehalose synthetase liquid is made；

It is 0.5%~1.0% in glutaraldehyde mass concentration, the volume mass ratio of trehalose synthetase liquid and chitosan gel rubber is 1:1 ~6:1, pH8.0, under conditions of 12 DEG C~25 DEG C of temperature, volume mass is ml/g than unit, and it is small to carry out crosslinking embedding reaction 8 When, immobilization trehalose synthetase is made；

Immobilization trehalose synthetase is mixed with maltose substrate solution, carbohydrase is then added, carbohydrase additional amount is 20U/ml adjusts pH 6.0, and control saccharification temperature is 65 DEG C, and enzymolysis time 8 hours, trehalose crude liquid was made in filtering；

The active carbon that addition accounts for trehalose crude liquid gross mass 1% into trehalose crude liquid decolourizes, and stirs, and filtering obtains two Secondary filtrate；

Standing 3 is small after 70% ethyl alcohol for 2 CO 3,10% of Na for accounting for secondary filtrate gross mass 0.6% is added into secondary filtrate When, filtering, the aqueous trehalose handled well.

Claims (10)

1. a kind of efficient trehalose extract equipment, including drying crystalline device, it is characterised in that: drying crystalline device includes lifting Module (1) releases module (2), main body module (3) and collection module (4)；Main body module (3) includes the hopper of upper end opening (24), hopper (24) inside bottom is equipped with heated at constant temperature piece (18), and hopper (24) left side wall is connected with feed pipe (15), hopper (24) Internal left and right side is symmetrically fixed with left and right heating box part, and Zuo Jiare box part includes being in turn secured to hopper from top to bottom Four left heater boxes (17) on interior left side, right heating box part include four left heater boxes in right heater box (27) lower side (17) above feed pipe (15), hopper (24) left and right lateral wall is designed with the exhaust fan (16) that air hose is in hopper, takes out Blower (16) is in above top side heater box (17)；Lifting module (1) includes the single shaft for being equipped with vertical uniaxial driver (5) Driver rack (6) is equipped with drying that is vertical and moving up and down under uniaxial driver (5) driving on uniaxial driver (5) Case connecting rod (7), drying box connecting rod (7) bottom end is fixed dry tank module (8), and dry tank module (8) are connected in company with drying box Bar (7) moves up and down in hopper (24) in the case where uniaxial driver (5) drive, and is equipped with from top to bottom in dry tank module (8) more Top, bottom surface are all fixed with absorbent cloth (22) inside a hothouse (21) and each hothouse (21)；It includes horizontal horizontal for releasing module (2) To the cylinder (9) of arrangement, the push rod (10) of cylinder (9) is to the right and push rod (10) right end is fixed with fixed block (12), fixed block (12) right end fixes the scraper plate fixed link (13) of more horizontal cross arrangement from top to bottom, and each scraper plate fixed link (13) right end is solid Surely it is connected with scraper plate (14), the quantity of scraper plate (14) and corresponding scraper plate fixed link (13) is consistent with hothouse (21) quantity, and scraper plate (14) structure and size are adapted with the structure and size of corresponding hothouse (21)；Collection module is equipped on the right side of main body module (3) (4), collection module (4) includes the collecting board (19) for collecting the scraped seaweed sugar crystal from absorbent cloth (22), collecting board (19) lower right is equipped with collecting box (20).

2. a kind of efficient trehalose extract equipment according to claim 1, it is characterised in that: four left sides from top to bottom add The heating temperature of hot tank (17) successively controls as 50 DEG C, 55 DEG C, 60 DEG C, 70 DEG C, and the heating temperature control of heated at constant temperature piece (18) It is made as 80 DEG C.

3. a kind of efficient trehalose extract equipment according to claim 1, it is characterised in that: uniaxial driver rack (6) Be arranged vertically on ground and in left part, right part, lower openings U-typed framework, uniaxial driver rack (6) includes vertical The topmast column (30) of front standing pillar (28) and back prop (29) and level, uniaxial driver rack (6) integral erection is in hopper (24) top, front standing pillar (28) are set on front side of hopper (24) front side wall, and back prop (29) is set on rear side of hopper (24) rear wall, single Axis driver rack (6) top relative position is equipped with horizontal longitudinally arranged cross between front standing pillar (28) and back prop (29) Beam, crossbeam are in above hopper (24), are equipped with vertical support in the middle part of beam surface upper between the middle part of topmast column (30) lower surface Column (31), vertical support column (31) right side are fixed with the uniaxial driver (5) arranged vertically；It is installed on uniaxial driver (5) There is uniaxial driver slide unit (25) and uniaxial driver slide unit (25) is freely up and down moved in uniaxial driver (5) stroke range Dynamic, uniaxial driver slide unit (25) right side is fixed with the drying box connecting rod (7) arranged vertically and drying box connecting rod (7) bottom End is fixedly connected with the center of dry tank module (8) top surface, drying box connecting rod (7) and with drying box connecting rod (7) are fixed connects The drying tank module (8) connect follows uniaxial driver slide unit (25) freely up and down to move.

4. a kind of efficient trehalose extract equipment according to claim 1, it is characterised in that: dry tank module (8) include Horizontally disposed and left end, the cuboid of right-end openings are dry cassette frame (23), are equipped with from top to bottom in dry cassette frame (23) The drying box reticle (26) of multiple horizontal cross arrangements, each drying box reticle (26) front end is and in dry cassette frame (23) Portion's leading flank is fixedly connected, and drying box reticle (26) rear end is fixedly connected with dry cassette frame (23) inner back side face, respectively Drying box reticle (26) left and right end is flushed with dry cassette frame (23) left and right end respectively, multiple drying box reticle (26) edges Vertical direction is uniformly distributed will be divided into from top to bottom inside drying box multiple hothouses (21), multiple hothouse (21) structure phases Fixation is equipped with absorbent cloth (22) respectively for same and each hothouse (21) internal top surface and bottom surface, the water suction of hothouse (21) top surface There are certain interval, each scraper plate (14) companions between cloth (22) lower surface and absorbent cloth (22) upper surface of hothouse (21) bottom surface Corresponding scraper plate fixed link (13) enters in corresponding hothouse (21) to the right in the case where push rod (10) drive, to corresponding hothouse (21) Absorbent cloth (22) lower surface of top surface and absorbent cloth (22) upper surface of corresponding hothouse (21) bottom surface carry out scraper simultaneously.

5. a kind of efficient trehalose extract equipment according to claim 4, it is characterised in that: in each left heater box (17) Equipped with left electric heating wire and left electric heating wire controls between the left and right heating box part of heating temperature of corresponding left heater box (17) Distance and dry cassette frame (23) horizontal cross distance are adapted, and left heater box (17) is the rectangular box and opening of right openings Size, shape are adapted with hothouse (21) left and right side opening.

6. a kind of efficient trehalose extract equipment according to claim 1, it is characterised in that: fixed block (12) is vertical cloth It sets, shape, construction, the size of each scraper plate fixed link (13) are all the same, and shape, construction, the size of each scraper plate (14) are all the same, and Scraper plate (14) is vertical and arranges along the longitudinal direction, the water suction with corresponding hothouse (21) top surface respectively of each scraper plate (14) top and bottom ends Absorbent cloth (22) the upper surface contact of cloth (22) lower surface, corresponding hothouse (21) bottom surface, while each scraper plate (14) is accordingly being done Left and right moves freely in dry room (21), and scraper plate fixed link (13) length is more than hothouse (21) horizontal cross length and diameter is less than Corresponding hothouse (21) is vertical highly, the vertical thickness in vertical gap and drying box reticle (26) between adjacent scraper plate (14) It is adapted.

7. a kind of efficient trehalose extract equipment according to claim 4, it is characterised in that: hopper (24) right side wall top It is fixed with the collecting board (19) of inclination arrangement, high right side is low on the left of collecting board (19), and collecting board (19) right end is arranged right below The collecting box (20) of portion's opening, dry cassette frame (23) rise to each scraper plate (14) when highest position and enter corresponding hothouse (21) with scraper in, collecting board (19) left end is at the lower right of the drying cassette frame (23) when rising to highest position.

8. a kind of efficient trehalose extract equipment according to claim 1, it is characterised in that: hopper (24) is horizontal positioned Rectangular box in ground, heating sheet (18) is set to hopper (24) inside bottom and protrudes inwardly from along peripheral wall, in heating sheet (18) Side bulk is greater than dry tank module (8) size, and heating sheet (18) is the heating sheet that electric heating wire is formed, and heating sheet (18) is interior Electric heating wire control heating sheet (18) heating temperature；The structure of exhaust fan (16), position are symmetrical in left and right side walls； Cylinder (9) is fixedly connected with hopper (24) by " L " type air cylinder support (11).

9. bacterium producing multi enzyme preparation, is first inoculated in fermentation medium by a kind of extracting method of above-mentioned efficient trehalose extract equipment, system Obtain fermentation liquid；By fermentation liquid obtained through thallus separation, thallus broken wall, enzyme purification, trehalose synthetase liquid is made；By trehalose Synthesis enzyme solution carries out crosslinking embedding with chitosan gel rubber and reacts obtained immobilization trehalose synthetase；Immobilization trehalose is synthesized Enzyme is mixed with maltose substrate solution, and aqueous trehalose is made in inverted, separation；Then a kind of above-mentioned efficient trehalose is used For extract equipment by aqueous trehalose drying crystalline, the step of drying crystalline, is as follows:

S1, the aqueous trehalose handled well is transported in hopper (24) by feed pipe (15), makes aqueous trehalose liquid level low In feed pipe (15) height, starting heating sheet (18) and controlling the heating temperature of heating sheet (18) is 80 DEG C, aqueous trehalose quilt Heating sheet (18) heating, and start uniaxial driver (5) so that uniaxial driver slide unit (25) declines and drives drying box connecting rod (7) decline, drying box connecting rod (7) drives dry tank module (8) decline in turn, and uniaxial driver slide unit (25) drops under stroke When dead point, dry tank module (8) are sunk to completely in the aqueous trehalose in heated condition, the absorbent cloth in hothouse (21) (22) aqueous trehalose is absorbed；

S2, after a period of time, dry tank module (8) slowly rise in company with uniaxial driver slide unit (25), uniaxial driver slide unit (25) the extra moisture of absorbent cloth (22) is dropped back into again in the aqueous trehalose of hopper (24) bottom in rising, dry tank module (8) rising to keeps dry tank module (8) top layer's hothouse (21) corresponding with lowest level left heater box (17) on the inside of hopper (24) When dry tank module (8) stop rising, left heater box (17), top layer's hothouse (21) and right heater box (27) surround phase together To closed cabinet, then start all left heater boxes (17), right heater box (27), and makes four left heater boxes from top to bottom Heating temperature successively control and allow top layer's hothouse (21) to stop one section in 70 DEG C of environment for 50 DEG C, 55 DEG C, 60 DEG C, 70 DEG C Time；

S3, after a period of time, dry tank module (8) continue slowly to rise in company with uniaxial driver slide unit (25), make drying box mould Block (8) top layer's hothouse (21) is corresponding with the second layer left heater box (17) are counted on the inside of hopper (24) from bottom to top, while making to do Dry tank module (8) from top to bottom on the inside of second layer hothouse (21) and hopper (24) the left heater box of lowest level (17) to it is corresponding when Tank module (8) stops rising, and top layer's hothouse (21) is allowed to stay for some time in 60 DEG C of environment；

S4, after a period of time, dry tank module (8) continue slowly to rise in company with uniaxial driver slide unit (25), make drying box mould Counted from bottom to top on the inside of block (8) top layer's hothouse (21) and hopper (24) the left heater boxes of third layer (17) to it is corresponding when mould Block (8) stops rising, and top layer's hothouse (21) is allowed to stay for some time in 55 DEG C of environment；

S5, after a period of time, dry tank module (8) continue slowly to rise in company with uniaxial driver slide unit (25), make drying box mould On the inside of block (8) top layer's hothouse (21) and hopper (24) the left heater box of top (17) to it is corresponding when module (8) stopping Rise, top layer's hothouse (21) allowed to stay for some time in 50 DEG C of environment, and so on, dry tank module (8) on to Under each layer of hothouse (21) all from top to bottom successively by the left heater box of each layer (17), corresponding right heater box (27) and Every layer of left heater box (17), corresponding right heater box (27) all stay for some time；

S6, when lowest level hothouse (21) is after the left heater box of top layer (17), corresponding right heater box (27) stay for some time, Uniaxial driver slide unit (25), which rises, is further driven to dry tank module (8) rising, when dry tank module (8) and exhaust fan (16) Stop rising when height is parallel, start exhaust fan (16), exhaust fan (16) carries out air-dried, absorbent cloth (22) to each hothouse (21) On start trehalose crystallization occur, it is uniaxial when the moisture evaporating completely on absorbent cloth (22) and when seaweed sugar crystal is no longer precipitated Driver slide unit (25) rising is further driven to dry tank module (8) and rises；

S7, when scraper plate (14) and corresponding hothouse (21) it is horizontal to it is corresponding when driver slide unit (25) stop rising, push rod (10) fixed block (12) are pushed to move right, fixed block (12) drives each scraper plate fixed link (13) and corresponding scraper plate (14) to transport to the right Dynamic, the multiple scraper plates (14) being vertically arranged are also cross the multiple hothouses (21) being vertically arranged, scraper plate (14) top and corresponding Hothouse (21) top surface absorbent cloth (22) following table face contact, scraper plate (14) bottom surface and corresponding hothouse (21) bottom surface absorbent cloth (22) Upper surface contact while scraper plate (14) moves right, the trehalose being precipitated on absorbent cloth (22) crystallization is scraped, trehalose knot Crystalline substance follows scraper plate (14) to move right together, finally falls on collecting board (19), and the trehalose on collecting board (19) is along collection Plate (19) slides, and falls in collecting box (20).

10. a kind of extracting method of efficient trehalose extract equipment according to claim 9, which is characterized in that trehalose Solution is produced including specific step is as follows:

First bacterium producing multi enzyme preparation is inoculated in fermentation medium, under conditions of 28 DEG C ~ 33 DEG C of temperature, 30h ~ 80h is cultivated, hair is made Zymotic fluid；

Fermentation liquid is carried out refrigerated centrifuge 1 hour under the conditions of 10 DEG C, the wet thallus obtained after centrifugation is collected, by wet thallus and phosphorus Sour sodium buffer mixing, then high pressure homogenizer is used, the broken of thallus is carried out, carries out the refrigerated centrifuge one under the conditions of 10 DEG C again Hour, collecting resulting supernatant is crude enzyme liquid；

Solid ammonium sulfate powder is added in crude enzyme liquid, 3 hours is stood after stirring, takes supernatant, trehalose synthetase liquid is made；

It is 0.5%~1.0% in glutaraldehyde mass concentration, the volume mass ratio of trehalose synthetase liquid and chitosan gel rubber is 1:1 ~6:1, pH8.0, under conditions of 12 DEG C~25 DEG C of temperature, volume mass is ml/g than unit, and it is small to carry out crosslinking embedding reaction 8 When, immobilization trehalose synthetase is made；

Immobilization trehalose synthetase is mixed with maltose substrate solution, carbohydrase is then added, carbohydrase additional amount is 20U/ml adjusts pH 6.0, and control saccharification temperature is 65 DEG C, and enzymolysis time 8 hours, trehalose crude liquid was made in filtering；

The active carbon that addition accounts for trehalose crude liquid gross mass 1% into trehalose crude liquid decolourizes, and stirs, and filtering obtains two Secondary filtrate；

Standing 3 is small after 70% ethyl alcohol for 2 CO 3,10% of Na for accounting for secondary filtrate gross mass 0.6% is added into secondary filtrate When, filtering, the aqueous trehalose handled well.