CN110250330A - A kind of Chinese herbal feed additive improving broiler chicken bone - Google Patents
A kind of Chinese herbal feed additive improving broiler chicken bone Download PDFInfo
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- CN110250330A CN110250330A CN201910499323.2A CN201910499323A CN110250330A CN 110250330 A CN110250330 A CN 110250330A CN 201910499323 A CN201910499323 A CN 201910499323A CN 110250330 A CN110250330 A CN 110250330A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
Abstract
The present invention relates to a kind of Chinese herbal feed additives for improving broiler chicken bone, belong to chicken feed addictive field, the Chinese herbal feed additive of the improvement broiler chicken bone includes square foundation and prescription, square foundation comprises the following components in parts by weight: Caulis Spatholobi 10-15 parts, 10-15 parts of Asiatic plantain, 10-15 parts of Divine Comedy, 3-9 parts of hawthorn, 10-15 parts of rhizoma polygonati, 3-9 parts of the fruit of glossy privet, 3-12 parts of Semen Cuscutae, 3-9 parts of oyster, prescription comprises the following components in parts by weight: 10-20 parts of Rehmannia glutinosa, 3-9 parts of Herba Epimedii, 10-15 parts of psoralea corylifolia, 10-20 parts of the rhizome of davallia.The present invention provides a kind of Chinese herbal feed additive that can effectively enhance poultry skeleton development, reduce poultry osteopathy disease incidence using traditional Chinese medicine principle and poultry threpsology as foundation；And the application extraction process is used, the recovery rate of aqueous soluble active constituent can be improved, the loss of effective component during the extraction process is reduced.
Description
Technical field
The invention belongs to chicken feed addictive fields, specifically, being related to a kind of Chinese herbal feed for improving broiler chicken bone
Additive.
Background technique
Skeletal system is that the life system of supporting function is provided for organism, and important work is played in animal growth
With.It as commercial broiler quickly increases weight, the breeding of the high laying rate of laying hen, has a negative impact, influences to the skeleton development of family chicken
Carcass quality, currently without the drug or feed addictive of preferable prevention and treatment family chicken bone disease, and on a large amount of mankind and mouse
Studies have shown that Chinese herbal medicine in have effects that anti-bone resorption (catabolism), for reach effectively enhancing poultry skeleton development,
The effect for reducing poultry osteopathy disease incidence applies Chinese herbal medicine in the prevention and treatment of family chicken bone disease.
Summary of the invention
In order to overcome the problems in background technique, this application provides a kind of Chinese herbal feed additions for improving broiler chicken bone
Agent can effectively enhance poultry skeleton development, reduce poultry osteopathy disease incidence.
To achieve the above object, the present invention is implemented according to following technical scheme:
A kind of Chinese herbal feed additive improving broiler chicken bone, including square foundation and prescription, it is basic by weight calculating
Side: prescription=1:1.
The square foundation comprises the following components in parts by weight: Caulis Spatholobi 10-15 parts, and 10-15 parts of Asiatic plantain, Divine Comedy 10-
15 parts, 3-9 parts of hawthorn, 10-15 parts of rhizoma polygonati, 3-9 parts of the fruit of glossy privet, 3-12 parts of Semen Cuscutae, 3-9 parts of oyster.
The prescription comprises the following components in parts by weight: 10-20 parts of Rehmannia glutinosa, 3-9 parts of Herba Epimedii, and psoralea corylifolia 10-15
Part, 10-20 parts of the rhizome of davallia.
A kind of preparation method for the Chinese herbal feed additive improving broiler chicken bone, specific steps are as follows:
1) square foundation and each raw material of prescription are weighed respectively, is mixed to get compound prescription by weight 1:1, by compound prescription plus
Water carries out soak at room temperature, and amount of water is 8-10 times, soaking time 60-80 minutes of compound prescription weight；
2) it completes to carry out continuous circumfluence extraction 3 times after impregnating, each extraction time is 30-40 minutes, will extract filter every time
Liquid is uniformly mixed；
3) the resulting filtrate of step 2) is condensed into medicinal extract, Chinese herbal feed additive is then made using freeze-drying.
Beneficial effects of the present invention: for the present invention using traditional Chinese medicine principle and poultry threpsology as foundation, providing one kind can
The effectively Chinese herbal feed additive of enhancing poultry skeleton development, reduction poultry osteopathy disease incidence；And work is extracted using the application
Skill can improve the recovery rate of aqueous soluble active constituent, reduce the loss of effective component during the extraction process.
Specific embodiment
Embodiment 1
A kind of Chinese herbal feed additive improving broiler chicken bone, including square foundation and prescription, it is basic by weight calculating
Side: prescription=1:1,5 kilograms of 5 kilograms+prescription of 10 kilograms of compound prescription=square foundations.
Wherein, the square foundation comprises the following components in parts by weight: 15 parts of Caulis Spatholobi, 15 parts of Asiatic plantain, and 12 parts of Divine Comedy,
9 parts of hawthorn, 10 parts of rhizoma polygonati, 9 parts of the fruit of glossy privet, 12 parts of Semen Cuscutae, 3 parts of oyster.The prescription comprises the following components in parts by weight:
20 parts of Rehmannia glutinosa, 9 parts of Herba Epimedii, 15 parts of psoralea corylifolia, 10 parts of the rhizome of davallia.
Preparation method are as follows:
1) square foundation and each raw material of prescription are weighed respectively, is mixed to get compound prescription by weight 1:1, by compound prescription plus
Water carries out soak at room temperature, and amount of water is 8 times, soaking time 60 minutes of compound prescription weight；
2) continuous circumfluence extraction 3 times repeatedly after impregnating are completed, each extraction time is 40 minutes, will extract every time filtrate and mix
It closes uniform；
3) the resulting filtrate of step 2) is condensed into medicinal extract, Chinese herbal feed additive is then made using freeze-drying.
Embodiment 2
A kind of Chinese herbal feed additive improving broiler chicken bone, including square foundation and prescription, it is basic by weight calculating
Side: prescription=1:1,5 kilograms of 5 kilograms+prescription of 10 kilograms of compound prescription=square foundations.
Wherein, the square foundation comprises the following components in parts by weight: 12 parts of Caulis Spatholobi, 15 parts of Asiatic plantain, and 12 parts of Divine Comedy,
9 parts of hawthorn, 15 parts of rhizoma polygonati, 9 parts of the fruit of glossy privet, 12 parts of Semen Cuscutae, 6 parts of oyster.The prescription comprises the following components in parts by weight:
12 parts of Rehmannia glutinosa, 9 parts of Herba Epimedii, 10 parts of psoralea corylifolia, 10 parts of the rhizome of davallia.
Preparation method are as follows:
1) square foundation and each raw material of prescription are weighed respectively, is mixed to get compound prescription by weight 1:1, by compound prescription plus
Water carries out soak at room temperature, and amount of water is 10 times, soaking time 60 minutes of compound prescription weight；
2) it completes after impregnating continuous circumfluence extraction 3 times, each extraction time is 30 minutes, and it is equal will to extract filtrate mixing every time
It is even；
3) the resulting filtrate of step 2) is condensed into medicinal extract, Chinese herbal feed additive is then made using freeze-drying.
Embodiment 3
A kind of Chinese herbal feed additive improving broiler chicken bone, including square foundation and prescription, it is basic by weight calculating
Side: prescription=1:1,5 kilograms of 5 kilograms+prescription of 10 kilograms of compound prescription=square foundations.
Wherein, the square foundation comprises the following components in parts by weight: 12 parts of Caulis Spatholobi, 15 parts of Asiatic plantain, and 12 parts of Divine Comedy,
9 parts of hawthorn, 15 parts of rhizoma polygonati, 9 parts of the fruit of glossy privet, 12 parts of Semen Cuscutae, 6 parts of oyster.The prescription comprises the following components in parts by weight:
13 parts of Rehmannia glutinosa, 6 parts of Herba Epimedii, 11 parts of psoralea corylifolia, 14 parts of the rhizome of davallia.
Preparation method are as follows:
1) square foundation and each raw material of prescription are weighed respectively, is mixed to get compound prescription by weight 1:1, by compound prescription plus
Water carries out soak at room temperature, and amount of water is 10 times, soaking time 80 minutes of compound prescription weight；
2) it completes continuous circumfluence extraction after impregnating to extract 3 times, each extraction time is 40 minutes, will extract every time filtrate and mix
It closes uniform；
3) the resulting filtrate of step 2) is condensed into medicinal extract, Chinese herbal feed additive is then made using freeze-drying.
Experimental analysis
Chinese herbal feed additive prepared by 2 Chinese herbal medicine formula of embodiment is applied in experimental group.
Experimental chicken chooses 240 section's treasured broiler chicken (the honest limited liability company in Kunming), is randomly divided into 4 groups, every group 60, divides
Not Wei control group (Chinese herbal feed additive is not added), compound low dose group (Chinese herbal feed additive additive amount be 1%) is multiple
Square middle dose group (Chinese herbal feed additive additive amount is 3%) and (the Chinese herbal feed additive addition of compound high dose group
5%) amount is.All experimental group basal diet formulas are identical with trophic level, and test chicken Basic drawing selects corn, dregs of beans, swollen
Changing soybean etc. is raw material, and for trophic level referring to Chinese fowl raising standard (2004), Screening Experiment of Diet is shown in Table 1.
1 daily ration of table composition and trophic level
Note: mineral additive provides for every kg diet: iron 80.00mg；Copper 20.00mg；Manganese 80.00mg；Zinc
100.00mg；Iodine 0.40mg；Selenium 0.30mg.
When 30,60 age in days, 20 test chickens similar in every group selection weight carry out at 3 points by universal testing machine
Bend test detects the bone strength of shin bone；Using Biochemical Analyzer detection bone metabolism associated blood biochemical indicator (VDR, OT,
OPG, ALP), with Real-Time-PCR method detection thoracic vertebrae, OPG, VDR in leg cartilaginous tissue, Runx-2, RANK, RANKL,
The difference of TNF-α, BMP-2 gene mRNA expression amount.Comparative experiments group and control group bone strength, associated blood biochemical indicator, phase
Correlation gene expression difference.
Experimental result
1. live-weight comparative analysis
Live-weight comparative analysis between table 2 30,60 age in days difference groups
Note: between same treatment group different days, * indicates that comparing difference is significant (p < 0.05), and * * indicates poor heteropolar aobvious
It writes (p < 0.01)；Between identical age in days different disposal group, lowercase indicates that comparing difference is significant (p < 0.05), and capitalization indicates
More heteropolar significant (p < 0.01)；Between each processing group of identical age in days and control group, # indicates that comparing difference is significant (p < 0.05), ##
Indicate that comparing difference is extremely significant (p < 0.01).(similarly hereinafter)
As can be seen from Table 2, between each processing group of identical age in days and control group: 30 age in days compound Chinese herb groups and control group are poor
Different not significant (P > 0.05).Between identical age in days different disposal group: 30 age in days compound Chinese herb middle dose groups are significantly higher than in compound
Herbal medicine low dose group (P < 0.05).
30 age in days compound Chinese herbs are high, middle dose group live-weight is above control group；60 age in days compound Chinese herbs are high, medium and low
Dosage group is slightly above control group, and the weight gain of compound Chinese herb high dose group is most obvious.On the whole, compound Chinese herb high dose adds
Add gaining effect after feeding experiment chicken best.
2. vitamin D receptor (VDR) comparision contents are analyzed
VDR comparision contents are analyzed in serum between table 3 30,60 age in days difference groups
As can be seen from Table 3, between each processing group of identical age in days and control group: VDR in 30 age in days compound Chinese herb group serum
Content is above control group；VDR content is above control group in 60 age in days compound Chinese herb group serum, and compound Chinese herb it is high,
In, low dose group be significantly higher than control group (P < 0.05).VDR content is all higher than control group in each processing group serum of 30 ages in days, and
Compound Chinese herb high dose group > Chinese herbal medicine low dose group > Chinese herbal medicine middle dose group；Each processing group serum VDR content of 60 ages in days
All it is higher than control group, and compound Chinese herb high dose group > Chinese herbal medicine middle dose group > Chinese herbal medicine low dose group.First and last, compound
VDR content highest in Chinese herbal medicine high dose group serum.
3. osteocalcin (OT) comparision contents are analyzed
OT comparision contents are analyzed in serum between table 4 30,60 age in days difference groups
As can be seen from Table 4, between each processing group of identical age in days and control group: 30 age in days compound Chinese herb high dose group serum
Middle OT content is higher than control group, but without significant difference (P > 0.05)；The 60 high, medium and low dosage groups of age in days compound Chinese herb are significantly high
In control group (P < 0.05).Between same treatment group different days: OT content is below 60 ages in days in 30 age in days serum.
OT content is all higher than control group in each processing group serum of 60 ages in days, and in compound Chinese herb high dose group > Chinese herbal medicine
Dosage group > Chinese herbal medicine low dose group.First and last, OT content highest in compound Chinese herb high dose group serum, to raising serum
Middle OT content is the most obvious.
4. osteoprotegerin (OPG) comparision contents are analyzed
OPG comparision contents are analyzed in serum between table 5 30,60 age in days difference groups
As can be seen from Table 5, between each processing group of identical age in days and control group: 30 ages in days remove compound Chinese herb middle dose group
Outside, OPG content is above control group in other processing group serum；OPG content is above control in each processing group serum of 60 ages in days
Group.Between same treatment group different days: OPG content is above 60 ages in days (P < 0.05) in 30 age in days serum.
OPG content is higher than control group, and compound Chinese herb high dose in 30 age in days compound Chinese herb high and low dose group serum
Group > Chinese herbal medicine low dose group；OPG content is all higher than control group in each processing group serum of 60 ages in days, and compound Chinese herb high dose group >
Chinese herbal medicine low dose group > Chinese herbal medicine middle dose group.First and last, in compound Chinese herb group high dose group to improve serum in OPG
Content is best.
5. alkaline phosphatase (ALP) comparision contents are analyzed
ALP comparision contents are analyzed in serum between table 6 30,60 age in days difference groups
As can be seen from Table 6, between each processing group of identical age in days and control group: 30 age in days compound Chinese herb high dose group serum
Middle ALP content is higher than control group；ALP content is above control group, compound Chinese herb high dose in each processing group serum of 60 ages in days
Sex differernce (P < 0.05) is write and there is limit with control group in group.30 age in days compound Chinese herb high dose groups are significantly higher than compound Chinese herb
In, low dose group (P < 0.05).Between same treatment group different days: ALP content is above 60 ages in days in 30 age in days serum, and multiple
Square 30 age in days serum levels of ALP content of Chinese herbal medicine high dose group is significantly higher than 60 ages in days (P < 0.05), ALP in 30 age in days serum of control group
Content is significantly higher than 60 ages in days (P < 0.05).
ALP content is higher than control group in 30 age in days compound Chinese herb high dose group serum；In each processing group serum of 60 ages in days
ALP content is above control group.First and last, high dose group effect is best in compound Chinese herb group.
6. elasticity modulus compares analysis
Shin bone elasticity modulus compares analysis between table 7 30,60 age in days difference groups
As can be seen from Table 7, between each processing group of identical age in days and control group: each processing group shin bone elasticity modulus of 30 ages in days is equal
Higher than control group, but there was no significant difference (P > 0.05)；Each processing group shin bone elasticity modulus of 60 ages in days is above control group, but poor
Different not significant (P > 0.05).It is anisotropic (P > 0.05) without significance difference between each processing group of 30 and 60 ages in days.Same treatment group is not on the same day
Between age: 30 age in days shin bone elasticity modulus are above 60 ages in days.
Each processing group shin bone elasticity modulus of 30 ages in days is above control group, compound Chinese herb high dose elasticity modulus highest；
60 age in days compound Chinese herb high dose group shin bone elasticity modulus highests are higher than control group.First and last, compound Chinese herb high dose
Group shin bone elasticity modulus highest, to improving, shin bone elasticity modulus effect is optimal.
7. yield strength compares analysis
Shin bone yield strength compares analysis between table 8 30,60 age in days difference groups
As can be seen from Table 8,30 age in days Chinese herbal medicine processing group shin bone yield strengths are above control group, but poor without conspicuousness
Different (P > 0.05)；In 60 age in days compound Chinese herbs, low dose group shin bone yield strength is lower than outside control group.Same treatment group is different
Between age in days: 30 age in days shin bone yield strengths are above 60 ages in days (P < 0.05).
30 age in days shin bone yield strength compound Chinese herb high dose groups > Chinese herbal medicine middle dose group > Chinese herbal medicine low dose group；60
Age in days compound Chinese herb high dose group > Chinese herbal medicine middle dose group > Chinese herbal medicine low dose group.First and last, the high agent of compound Chinese herb
It is most strong to bear ambient pressure ability for amount group shin bone yield strength highest.
8. maximum stress comparative analysis
Shin bone maximum stress comparative analysis between table 9 30,60 age in days difference groups
As can be seen from Table 9, between each processing group of identical age in days and control group: 30 age in days compound Chinese herbs height, middle dose group
Shin bone maximum stress is higher than control group, but there was no significant difference (P > 0.05)；60 ages in days remove compound Chinese herb low dose group shin bone
Maximum stress is lower than outside control group, and other groups are above control group, but difference is not significant (P > 0.05).30 ages in days and 60 ages in days
Each processing group there are no significant difference (P > 0.05).Between same treatment group different days: 30 age in days shin bone maximum stresses are above
60 ages in days, and 30 age in days shin bone yield strength of compound Chinese herb high and low dose group is significantly higher than 60 ages in days (P < 0.05).
30 age in days shin bone maximum stress compound Chinese herb high dose groups are higher than control group；60 age in days compound Chinese herb high doses
Group > control group.First and last, high dose group maximum stress is maximum in compound Chinese herb group.
The analysis of 9.OPG gene relative expression quantity comparison in difference
Different group OPG gene relative expression quantities in table 10 30,60 age in days thoracic vertebrae tissues
As can be seen from Table 10, between identical age in days difference group: OPG base in 30 age in days compound Chinese herb group thoracic vertebrae tissues
Because mRNA relative expression quantity is all higher than control group；OPG gene mRNA relative expression in 60 age in days compound Chinese herb group thoracic vertebrae tissues
Amount is all higher than control group, and compound Chinese herb is high, middle dose group OPG gene mRNA relative expression quantity is control group 1.6 times
More than.Between identical group Different Month: OPG gene mRNA phase in the high, medium and low 30 age in days thoracic vertebrae tissue of dosage group of compound Chinese herb
60 ages in days are above to expression quantity.
Different group OPG gene relative expression quantities in table 11 30,60 age in days leg cartilaginous tissues
It can be seen that from table 11, between identical age in days difference group: the high, medium and low dosage group leg of 30 age in days compound Chinese herbs is soft
OPG gene mRNA relative expression quantity is higher than control group in bone tissue, and phase is all presented in the high, medium and low dosage group of compound Chinese herb
Successively decrease situation to expression quantity；OPG gene mRNA relative expression quantity in 60 age in days compound Chinese herbs height, middle dose group leg cartilaginous tissue
It is 1 times or more of control group, and compound Chinese herb high dose group is 1.83 times of control group.Between identical group Different Month:
In addition to compound Chinese herb high dose group, other each groups are all OPG gene mRNA relative expression quantities in 30 age in days leg cartilaginous tissues
It is above 60 ages in days.
On the whole, Chinese herbal medicine high dose group OPG gene relative expression quantity highest, explanation in thoracic vertebrae and leg cartilaginous tissue
Chinese herbal medicine high dose has the function of promoting OPG gene expression, and effect is optimal in Chinese herbal medicine group.10.VDR gene phase
To expression difference comparative analysis
Different group VDR gene relative expression quantities in table 12 30,60 age in days thoracic vertebrae tissues
It can be seen that from table 12, between identical age in days difference group: VDR base in 30 age in days compound Chinese herb group thoracic vertebrae tissues
Because mRNA relative expression quantity is all higher than control group, and compound Chinese herb is high, VDR gene mRNA is opposite in middle dose group thoracic vertebrae tissue
Expression quantity is 3.68 times of control group or more, and Chinese herbal medicine group relative expression quantity is in high dose > middle dosage > low dosage；60 ages in days
Each processing group VDR relative expression quantity is above control group.Between identical group Different Month: the high, medium and low dosage group of compound Chinese herb
VDR gene mRNA relative expression quantity is above 60 ages in days in 30 age in days thoracic vertebrae tissues.
Different group VDR gene relative expression quantities in table 13 30,60 age in days leg cartilaginous tissues
It can be seen that from table 13, between identical age in days difference group: the high, medium and low dosage group leg of 30 age in days compound Chinese herbs is soft
VDR gene mRNA relative expression quantity is below control group in bone tissue；The high, medium and low dosage group leg of 60 age in days compound Chinese herbs is soft
VDR gene mRNA relative expression quantity is below control group in bone tissue.Between identical group Different Month: except compound Chinese herb is high,
Outside low dose group, other each groups are all that VDR gene mRNA relative expression quantity is above 60 ages in days in 30 age in days leg cartilaginous tissues.
The analysis of 11.Runx-2 gene relative expression quantity comparison in difference
Different group Runx-2 gene relative expression quantities in table 14 30,60 age in days thoracic vertebrae tissues
It can be seen that from table 14, between identical age in days difference group: Runx-2 in 30 age in days compound Chinese herb group thoracic vertebrae tissues
Gene mRNA relative expression quantity is all higher than control group, and compound Chinese herb is high, Runx-2 gene in middle dose group thoracic vertebrae tissue
MRNA relative expression quantity is 5 times of control group or more；The high, medium and low dosage group Runx-2 gene of 60 age in days compound Chinese herbs
MRNA relative expression quantity is above control group, and Runx-2 gene mRNA relative expression in compound Chinese herb high dose thoracic vertebrae tissue
Amount is 3.13 times of control group.Between identical group Different Month: in addition to compound Chinese herb low dose group, compound Chinese herb is high, in
Runx-2 gene mRNA relative expression quantity is above 60 ages in days in 30 age in days thoracic vertebrae tissue of dosage group.
Different group Runx-2 gene relative expression quantities in table 15 30,60 age in days leg cartilaginous tissues
It can be seen that from table 15, between identical age in days difference group: the high, medium and low dosage group leg of 30 age in days compound Chinese herbs is soft
Runx-2 gene mRNA relative expression quantity is above control group in bone tissue；The high, medium and low dosage group leg of 60 age in days compound Chinese herbs
Runx-2 gene mRNA relative expression quantity is above control group in cartilaginous tissue, and in compound Chinese herb group leg cartilaginous tissue
Runx-2 gene mRNA relative expression quantity is in high dose > middle dosage > low dosage trend.Between identical group different days: each group
It is all that Runx-2 gene mRNA relative expression quantity is above 60 ages in days in 30 age in days leg cartilaginous tissues.
First and last, either 30 ages in days or 60 ages in days, compound Chinese herb high dose group in thoracic vertebrae, leg cartilaginous tissue
Runx-2 gene relative expression quantity is all higher than other groups, illustrates that Chinese herbal medicine high dose group has the effect for promoting Runx-2 gene expression
Fruit.
The analysis of 12.RANK gene relative expression quantity comparison in difference
Different group RANK gene relative expression quantities in table 16 30,60 age in days thoracic vertebrae tissues
It can be seen that from table 16, between identical age in days difference group: RANK base in 30 age in days compound Chinese herb group thoracic vertebrae tissues
Because mRNA relative expression quantity is below control group, and RANK gene mRNA is opposite in compound Chinese herb high dose group thoracic vertebrae tissue
Expression quantity is only 0.14 times of control group；The high, medium and low dosage group RANK gene mRNA relative expression quantity of 60 age in days compound Chinese herbs
It is below control group, and compound Chinese herb group low, middle and high dose groups RANK gene mRNA relative expression quantity tapers off trend.Phase
With between group different days: RANK gene mRNA relative expression in the high, medium and low 30 age in days thoracic vertebrae tissue of dosage group of compound Chinese herb
Amount is below 60 ages in days.
Different group RANK gene relative expression quantities in table 17 30,60 age in days leg cartilaginous tissues
It can be seen that from table 17, between identical age in days difference group: the high, medium and low dosage group leg of 30 age in days compound Chinese herbs is soft
RANK gene mRNA relative expression quantity is below control group in bone tissue；The high, medium and low dosage group leg of 60 age in days compound Chinese herbs is soft
RANK gene mRNA relative expression quantity is below control group, and RANK gene in compound Chinese herb group leg cartilage bone tissue in tissue
MRNA relative expression quantity is in low dosage > middle metering > high dose trend.Between identical group different days: each group is all 30 ages in days
RANK gene mRNA relative expression quantity is below 60 ages in days in leg cartilaginous tissue.
First and last, in thoracic vertebrae and leg cartilaginous tissue, 30,60 age in days Chinese herbal medicine high dose group RANK gene relative expression quantities
Other groups are below, illustrate that Chinese herbal medicine high dose group has effects that inhibit RANK gene expression.
The analysis of 13.RANKL gene relative expression quantity comparison in difference
Different group RANKL gene relative expression quantities in table 18 30,60 age in days thoracic vertebrae tissues
It can be seen that from table 18, between identical age in days difference group: RANKL base in 30 age in days compound Chinese herb group thoracic vertebrae tissues
Because mRNA relative expression quantity is below control group；The high, medium and low dosage group RANKL gene mRNA of 60 age in days compound Chinese herbs is opposite
Expression quantity is below control group；And 30,60 RANKL gene mRNA in the high, medium and low dosage group thoracic vertebrae tissue of age in days compound Chinese herb
Low dosage > middle metering > high dose trend is presented in relative expression quantity.Between identical group different days: compound Chinese herb is high, in,
RANKL gene mRNA relative expression quantity is below 60 ages in days in 30 age in days thoracic vertebrae tissue of low dose group.
Different group RANKL gene relative expression quantities in table 19 30,60 age in days leg soft tissues
It can be seen that from table 19, between identical age in days difference group: the high, medium and low dosage group leg of 30 age in days compound Chinese herbs is soft
RANKL gene mRNA relative expression quantity is below control group in bone tissue；The high, medium and low dosage group leg of 60 age in days compound Chinese herbs
RANKL gene mRNA relative expression quantity is below control group in cartilaginous tissue.Between identical group different days: removing compound Chinese herb
Outside middle dose group, other each groups are all that RANKL gene mRNA relative expression quantity is below 60 in 30 age in days leg cartilaginous tissues
Age.
The analysis of 14.TNF- α gene relative expression quantity comparison in difference
Different group TNF-α gene relative expression quantities in table 20 30,60 age in days thoracic vertebrae tissues
It can be seen that from table 20, between identical age in days difference group: TNF-α gene mRNA phase in 30 age in days compound Chinese herb groups
Control group is below to expression quantity；The high, medium and low dosage group TNF-α gene mRNA relative expression quantity of 60 age in days compound Chinese herbs is equal
Lower than control group；And 30 TNF-α gene mRNA relative expression quantity in the high, medium and low dosage group thoracic vertebrae tissue of age in days compound Chinese herb
Low dosage > middle dosage > high dose trend is presented.Between identical group different days: the high, medium and low dosage group 30 of compound Chinese herb
TNF-α gene mRNA relative expression quantity is below 60 ages in days in age in days thoracic vertebrae tissue.
Different group TNF-α gene relative expression quantities in table 21 30,60 age in days leg cartilaginous tissues
It can be seen that from table 21, between identical age in days difference group: the high, medium and low dosage group leg of 30 age in days compound Chinese herbs is soft
TNF-α gene mRNA relative expression quantity is below control group in bone tissue；The high, medium and low dosage group leg of 60 age in days compound Chinese herbs
TNF-α gene mRNA relative expression quantity is below control group in cartilaginous tissue.Between identical group different days: each group is all 30
TNF-α gene mRNA relative expression quantity is below 60 ages in days in age in days leg cartilaginous tissue.
The analysis of 15.BMP-2 gene relative expression quantity comparison in difference
Different group BMP-2 gene relative expression quantities in table 22 30,60 age in days thoracic vertebrae tissues
It can be seen that from table 22, between identical age in days difference group: BMP-2 in 30 age in days compound Chinese herb group thoracic vertebrae tissues
Gene mRNA relative expression quantity is all higher than control group, and compound Chinese herb is high, BMP-2 gene mRNA in middle dose group thoracic vertebrae tissue
Relative expression quantity is 2.5 times of control group or more；The high, medium and low dosage group BMP-2 gene mRNA phase of 60 age in days compound Chinese herbs
Control group is above to expression quantity, and BMP-2 gene mRNA relative expression quantity is in compound Chinese herb high dose thoracic vertebrae tissue
1.5 times or more of control group.Between identical group different days: in the high, medium and low 30 age in days thoracic vertebrae tissue of dosage group of compound Chinese herb
BMP-2 gene mRNA relative expression quantity is above 60 ages in days.
Different group BMP-2 gene relative expression quantities in table 23 30,60 age in days leg cartilaginous tissues
It can be seen that from table 23, between identical age in days difference group: the high, medium and low dosage group leg of 30 age in days compound Chinese herbs is soft
BMP-2 gene mRNA relative expression quantity is above control group in bone tissue, and compound Chinese herb is high, middle dose group leg cartilaginous tissue
Middle BMP-2 gene mRNA relative expression quantity is all 2 times of control group or so, BMP-2 gene in compound Chinese herb group leg cartilaginous tissue
MRNA relative expression quantity is in high dose > middle dosage > low dosage trend；The high, medium and low dosage group leg cartilage of 60 age in days compound Chinese herbs
BMP-2 gene mRNA relative expression quantity is above control group in tissue.Between identical group different days: removing low dose of compound Chinese herb
Outside amount group, other each groups are all that BMP-2 gene mRNA relative expression quantity is above 60 ages in days in 30 age in days leg cartilaginous tissues.
For the present invention using traditional Chinese medicine principle and poultry threpsology as foundation, poultry bone hair can effectively be enhanced by providing one kind
Educate, reduce the Chinese herbal feed additive of poultry osteopathy disease incidence；And using the technique of ultrasound extraction, improve water-soluble effective
The recovery rate of ingredient reduces the loss of effective component during the extraction process.
Finally, it is stated that preferred embodiment above is only used to illustrate the technical scheme of the present invention and not to limit it, although logical
It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (4)
1. a kind of Chinese herbal feed additive for improving broiler chicken bone, it is characterised in that: the medium-height grass of the improvement broiler chicken bone
Medicine feed addictive includes square foundation and prescription, by weight calculating square foundation: prescription=1:1.
2. a kind of Chinese herbal feed additive for improving broiler chicken bone according to claim 1, it is characterised in that: described
Square foundation comprises the following components in parts by weight: Caulis Spatholobi 10-15 parts, and 10-15 parts of Asiatic plantain, 10-15 parts of Divine Comedy, 3-9 parts of hawthorn,
10-15 parts of rhizoma polygonati, 3-9 parts of the fruit of glossy privet, 3-12 parts of Semen Cuscutae, 3-9 parts of oyster.
3. a kind of Chinese herbal feed additive for improving broiler chicken bone according to claim 1, it is characterised in that: described
Prescription comprises the following components in parts by weight: 10-20 parts of Rehmannia glutinosa, 3-9 parts of Herba Epimedii, and 10-15 parts of psoralea corylifolia, rhizome of davallia 10-20
Part.
4. a kind of preparation side of the Chinese herbal feed additive of improvement broiler chicken bone according to claim 1 to 3
Method, it is characterised in that: specific steps are as follows:
1) square foundation and each raw material of prescription are weighed respectively, is mixed to get compound prescription by weight 1:1, by compound prescription plus water into
Row soak at room temperature, amount of water are 8-10 times, soaking time 60-80 minutes of compound prescription weight；
2) it completes to carry out continuous circumfluence extraction 3 times after impregnating, each extraction time is 30-40 minutes, will extract every time filtrate and mix
It closes uniform；
3) the resulting filtrate of step 2 is condensed into medicinal extract, Chinese herbal feed additive is then made using freeze-drying.
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| CN103082138A (en) * | 2013-01-18 | 2013-05-08 | 山东省农业科学院家禽研究所 | Compound premix for promoting development of body sizes and ovaries of laying hens |
| CN103110779A (en) * | 2011-11-17 | 2013-05-22 | 广东紫金正天药业有限公司 | Composition containing Chinese yam and application thereof |
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| CN104489398A (en) * | 2014-12-23 | 2015-04-08 | 西安鑫诚大唐畜牧有限公司 | Additive of feed for meat chicken and preparation method of additive |
| CN109123181A (en) * | 2018-08-14 | 2019-01-04 | 播恩生物技术股份有限公司 | Improve laying hen bone density and the premixed feed of eggshell quality and preparation method thereof |
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| CN103110779A (en) * | 2011-11-17 | 2013-05-22 | 广东紫金正天药业有限公司 | Composition containing Chinese yam and application thereof |
| CN103082138A (en) * | 2013-01-18 | 2013-05-08 | 山东省农业科学院家禽研究所 | Compound premix for promoting development of body sizes and ovaries of laying hens |
| CN104171667A (en) * | 2014-07-30 | 2014-12-03 | 樊继绪 | Special composite chicken feed for laying hens in egg producing period |
| CN104489398A (en) * | 2014-12-23 | 2015-04-08 | 西安鑫诚大唐畜牧有限公司 | Additive of feed for meat chicken and preparation method of additive |
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