CN110243637B - 用于微流体装置分段装载的毛细屏障 - Google Patents

用于微流体装置分段装载的毛细屏障 Download PDF

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CN110243637B
CN110243637B CN201910521030.XA CN201910521030A CN110243637B CN 110243637 B CN110243637 B CN 110243637B CN 201910521030 A CN201910521030 A CN 201910521030A CN 110243637 B CN110243637 B CN 110243637B
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liquid
capillary
interface region
microfluidic channel
barrier
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CN110243637A (zh
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J·G·圣地亚哥
L·A·马歇尔
A·鲁格克斯
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Leland Stanford Junior University
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
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    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
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    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2300/00—Additional constructional details
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    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
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    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
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    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
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    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
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    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break
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CN201910521030.XA 2013-03-14 2014-03-14 用于微流体装置分段装载的毛细屏障 Active CN110243637B (zh)

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US201361785255P 2013-03-14 2013-03-14
US61/785,255 2013-03-14
CN201480021876.2A CN105190280B (zh) 2013-03-14 2014-03-14 用于微流体装置分段装载的毛细屏障
PCT/US2014/029017 WO2014153092A1 (en) 2013-03-14 2014-03-14 Capillary barriers for staged loading of microfluidic devices

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US (6) US20160160208A1 (enExample)
EP (1) EP2972185A4 (enExample)
JP (2) JP6673820B2 (enExample)
CN (2) CN110243637B (enExample)
AU (4) AU2014236262A1 (enExample)
CA (1) CA2906730C (enExample)
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Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2505706A (en) * 2012-09-10 2014-03-12 Univ Leiden Apparatus comprising meniscus alignment barriers
JP6673820B2 (ja) 2013-03-14 2020-03-25 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー マイクロ流体デバイスの段階的ロードのための毛細管バリア
US10117563B2 (en) * 2014-01-09 2018-11-06 Gyrus Acmi, Inc. Polyp detection from an image
JP7277050B2 (ja) 2016-01-29 2023-05-18 ピューリジェン バイオシステムズ， インコーポレイテッド 核酸の精製のための等速電気泳動
WO2017151098A1 (en) * 2016-02-29 2017-09-08 Hewlett-Packard Development Company, L.P. Liquid directing sample container
AU2017408261A1 (en) * 2017-04-05 2019-11-28 Dianax S.R.L. Lab-on-a-chip diagnosis device
AU2018312570B2 (en) * 2017-08-02 2024-01-11 Purigen Biosystems, Inc. Systems, devices, and methods for isotachophoresis
US12241907B2 (en) 2019-04-22 2025-03-04 Sekisui Chemical Co., Ltd. Chip and fluid-merging method
JP7164505B2 (ja) * 2019-10-02 2022-11-01 積水化学工業株式会社 マイクロ流路チップ
US20230294094A1 (en) * 2020-07-29 2023-09-21 Kyocera Corporation Flow channel device

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1326549A (zh) * 1998-10-13 2001-12-12 微生物系统公司 基于无源流体动力学的流体管路元件
CA2304641C (en) * 1997-09-26 2003-09-02 The Regents Of The University Of Michigan Moving and mixing of microdroplets through microchannels
CN1548957A (zh) * 2002-09-27 2004-11-24 ��տƼ��ɷ����޹�˾ 微流体传送及分析系统
CN1715929A (zh) * 2004-06-30 2006-01-04 生命扫描苏格兰有限公司 流控调节装置
EP1932593A1 (en) * 2006-11-22 2008-06-18 FUJIFILM Corporation Microchannel chip and converging device
EP2213364A1 (en) * 2009-01-30 2010-08-04 Albert-Ludwigs-Universität Freiburg Phase guide patterns for liquid manipulation

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4426451A (en) 1981-01-28 1984-01-17 Eastman Kodak Company Multi-zoned reaction vessel having pressure-actuatable control means between zones
EP0373192B1 (en) 1988-03-09 1996-06-12 The Board Of Trustees Of The Leland Stanford Junior University Diagnostic genes for toxoplasmosis
AU4248593A (en) 1992-05-14 1993-12-13 Mark Holodniy Polymerase chain reaction assays for monitoring antiviral therapy
GB9324310D0 (en) * 1993-11-26 1994-01-12 Univ Birmingham Liquid transfer device
US5827415A (en) 1994-09-26 1998-10-27 The Board Of Trustees Of Leland Stanford Jun. Univ. Oxygen sensor
US6143248A (en) 1996-08-12 2000-11-07 Gamera Bioscience Corp. Capillary microvalve
US6090251A (en) 1997-06-06 2000-07-18 Caliper Technologies, Inc. Microfabricated structures for facilitating fluid introduction into microfluidic devices
US5992820A (en) 1997-11-19 1999-11-30 Sarnoff Corporation Flow control in microfluidics devices by controlled bubble formation
US6601613B2 (en) 1998-10-13 2003-08-05 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
US6626417B2 (en) 2001-02-23 2003-09-30 Becton, Dickinson And Company Microfluidic valve and microactuator for a microvalve
US7429354B2 (en) 2001-03-19 2008-09-30 Gyros Patent Ab Structural units that define fluidic functions
US6581438B1 (en) * 2002-01-31 2003-06-24 Sandia Corporation Capillary test specimen, system, and methods for in-situ visualization of capillary flow and fillet formation
US6561224B1 (en) 2002-02-14 2003-05-13 Abbott Laboratories Microfluidic valve and system therefor
FR2848125B1 (fr) 2002-12-04 2006-06-09 Commissariat Energie Atomique Dispositif microfluidique dans lequel l'interface liquide/fluide est stabilisee
DE10302721A1 (de) * 2003-01-23 2004-08-05 Steag Microparts Gmbh Mikrofluidische Anordnung zum Dosieren von Flüssigkeiten
WO2006061026A2 (en) * 2004-12-09 2006-06-15 Inverness Medical Switzerland Gmbh A micro fluidic device and methods for producing a micro fluidic device
US20070014695A1 (en) 2005-04-26 2007-01-18 Applera Corporation Systems and Methods for Multiple Analyte Detection
EP1917097A2 (en) 2005-07-05 2008-05-07 Massachusetts Institute of Technology (MIT) Microfluidic separators for multiphase fluid-flow based on membranes
US8414754B1 (en) 2006-05-31 2013-04-09 The Board Of Trustees Of The Leland Stanford Junior University Electrophoretic sample analysis and approach therefor
US8562804B2 (en) 2006-07-20 2013-10-22 The Board Of Trustees Of The Leland Stanford Junior University Fluorescent finger prints for indirect detection in isotachophoresis
US7951278B2 (en) 2006-07-20 2011-05-31 The Board Of Trustees Of The Leland Standford Junior University Method of detecting directly undetectable analytes using directly detectable spacer molecules
US8999129B2 (en) 2011-03-07 2015-04-07 Lawrence Livermore National Security, Llc Liquid and gel electrodes for transverse free flow electrophoresis
WO2008124046A1 (en) 2007-04-04 2008-10-16 Micropoint Bioscience Inc.. Micromachined electrowetting microfluidic valve
US8852952B2 (en) * 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
US8394251B2 (en) 2008-10-07 2013-03-12 The Board Of Trustees Of The Leland Stanford Junior University Control of chemical reactions using isotachophoresis
US8846314B2 (en) 2009-03-03 2014-09-30 The Board Of Trustees Of The Leland Stanford Junior University Isotachophoretic focusing of nucleic acids
US9285340B2 (en) 2009-08-17 2016-03-15 Lawrence Livermore National Security, Llc Methods for separating particles and/or nucleic acids using isotachophoresis
US9144799B2 (en) 2009-11-24 2015-09-29 Lawrence Livermore National Security, Llc Modular microfluidic system for biological sample preparation
US8721858B2 (en) 2010-03-12 2014-05-13 The Board Of Trustees Of The Leland Stanford Junior University Non-focusing tracers for indirect detection in electrophoretic displacement techniques
US8512538B2 (en) * 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US8986529B2 (en) 2010-09-13 2015-03-24 The Board Of Trustees Of The Leland Stanford Junior University Isotachophoresis having interacting anionic and cationic shock waves
US9151732B2 (en) 2010-10-01 2015-10-06 The Board Of Trustees Of The Leland Stanford Junior University Enhanced isotachophoresis assays using additives with spatial gradients
US8524061B2 (en) 2010-11-29 2013-09-03 The Board Of Trustees Of The Leland Stanford Junior University On-chip hybridization coupled with ITP based purification for fast sequence specific identification
GB201103917D0 (en) * 2011-03-08 2011-04-20 Univ Leiden Apparatus for and methods of processing liquids or liquid based substances
US9057673B2 (en) 2012-08-24 2015-06-16 The Board Of Trustees Of The Leland Stanford Junior University Method of preparing RNA from ribonuclease-rich sources
JP6673820B2 (ja) 2013-03-14 2020-03-25 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー マイクロ流体デバイスの段階的ロードのための毛細管バリア
US11325123B2 (en) 2013-08-02 2022-05-10 The Board Of Trustees Of The Leland Stanford Junior University Flow regulation in fluidic systems using a phase-change material at system ports
JP7277050B2 (ja) 2016-01-29 2023-05-18 ピューリジェン バイオシステムズ， インコーポレイテッド 核酸の精製のための等速電気泳動

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2304641C (en) * 1997-09-26 2003-09-02 The Regents Of The University Of Michigan Moving and mixing of microdroplets through microchannels
CN1326549A (zh) * 1998-10-13 2001-12-12 微生物系统公司 基于无源流体动力学的流体管路元件
CN1548957A (zh) * 2002-09-27 2004-11-24 ��տƼ��ɷ����޹�˾ 微流体传送及分析系统
CN1715929A (zh) * 2004-06-30 2006-01-04 生命扫描苏格兰有限公司 流控调节装置
EP1932593A1 (en) * 2006-11-22 2008-06-18 FUJIFILM Corporation Microchannel chip and converging device
EP2213364A1 (en) * 2009-01-30 2010-08-04 Albert-Ludwigs-Universität Freiburg Phase guide patterns for liquid manipulation

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