CN110243637B - 用于微流体装置分段装载的毛细屏障 - Google Patents

用于微流体装置分段装载的毛细屏障 Download PDF

Info

Publication number
CN110243637B
CN110243637B CN201910521030.XA CN201910521030A CN110243637B CN 110243637 B CN110243637 B CN 110243637B CN 201910521030 A CN201910521030 A CN 201910521030A CN 110243637 B CN110243637 B CN 110243637B
Authority
CN
China
Prior art keywords
liquid
capillary
interface region
microfluidic channel
barrier
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201910521030.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN110243637A (zh
Inventor
J·G·圣地亚哥
L·A·马歇尔
A·鲁格克斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Leland Stanford Junior University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of CN110243637A publication Critical patent/CN110243637A/zh
Application granted granted Critical
Publication of CN110243637B publication Critical patent/CN110243637B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/088—Passive control of flow resistance by specific surface properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Electrochemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micromachines (AREA)
CN201910521030.XA 2013-03-14 2014-03-14 用于微流体装置分段装载的毛细屏障 Active CN110243637B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361785255P 2013-03-14 2013-03-14
US61/785,255 2013-03-14
PCT/US2014/029017 WO2014153092A1 (en) 2013-03-14 2014-03-14 Capillary barriers for staged loading of microfluidic devices
CN201480021876.2A CN105190280B (zh) 2013-03-14 2014-03-14 用于微流体装置分段装载的毛细屏障

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201480021876.2A Division CN105190280B (zh) 2013-03-14 2014-03-14 用于微流体装置分段装载的毛细屏障

Publications (2)

Publication Number Publication Date
CN110243637A CN110243637A (zh) 2019-09-17
CN110243637B true CN110243637B (zh) 2022-06-24

Family

ID=51581416

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201910521030.XA Active CN110243637B (zh) 2013-03-14 2014-03-14 用于微流体装置分段装载的毛细屏障
CN201480021876.2A Active CN105190280B (zh) 2013-03-14 2014-03-14 用于微流体装置分段装载的毛细屏障

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201480021876.2A Active CN105190280B (zh) 2013-03-14 2014-03-14 用于微流体装置分段装载的毛细屏障

Country Status (8)

Country Link
US (6) US20160160208A1 (enExample)
EP (1) EP2972185A4 (enExample)
JP (2) JP6673820B2 (enExample)
CN (2) CN110243637B (enExample)
AU (4) AU2014236262A1 (enExample)
CA (1) CA2906730C (enExample)
GB (1) GB2526999B (enExample)
WO (1) WO2014153092A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2505706A (en) * 2012-09-10 2014-03-12 Univ Leiden Apparatus comprising meniscus alignment barriers
US20160160208A1 (en) 2013-03-14 2016-06-09 The Board Of Trustees Of The Leland Stanford Junior University Capillary barriers for staged loading of microfluidic devices
US10117563B2 (en) * 2014-01-09 2018-11-06 Gyrus Acmi, Inc. Polyp detection from an image
AU2017212754B2 (en) 2016-01-29 2023-06-29 Purigen Biosystems, Inc. Isotachophoresis for purification of nucleic acids
US10888866B2 (en) 2016-02-29 2021-01-12 Hewlett-Packard Development Company, L.P. Liquid directing sample container
AU2017408261A1 (en) * 2017-04-05 2019-11-28 Dianax S.R.L. Lab-on-a-chip diagnosis device
SG11202000871WA (en) * 2017-08-02 2020-02-27 Purigen Biosystems Inc Systems, devices, and methods for isotachophoresis
US12241907B2 (en) 2019-04-22 2025-03-04 Sekisui Chemical Co., Ltd. Chip and fluid-merging method
JP7164505B2 (ja) * 2019-10-02 2022-11-01 積水化学工業株式会社 マイクロ流路チップ
JP7483892B2 (ja) * 2020-07-29 2024-05-15 京セラ株式会社 流路デバイス

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1326549A (zh) * 1998-10-13 2001-12-12 微生物系统公司 基于无源流体动力学的流体管路元件
CA2304641C (en) * 1997-09-26 2003-09-02 The Regents Of The University Of Michigan Moving and mixing of microdroplets through microchannels
CN1548957A (zh) * 2002-09-27 2004-11-24 ��տƼ��ɷ����޹�˾ 微流体传送及分析系统
CN1715929A (zh) * 2004-06-30 2006-01-04 生命扫描苏格兰有限公司 流控调节装置
EP1932593A1 (en) * 2006-11-22 2008-06-18 FUJIFILM Corporation Microchannel chip and converging device
EP2213364A1 (en) * 2009-01-30 2010-08-04 Albert-Ludwigs-Universität Freiburg Phase guide patterns for liquid manipulation

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4426451A (en) 1981-01-28 1984-01-17 Eastman Kodak Company Multi-zoned reaction vessel having pressure-actuatable control means between zones
WO1989008700A1 (en) 1988-03-09 1989-09-21 The Board Of Trustees Of The Leland Stanford Junio Diagnostic genes for toxoplasmosis
AU4248593A (en) 1992-05-14 1993-12-13 Mark Holodniy Polymerase chain reaction assays for monitoring antiviral therapy
GB9324310D0 (en) * 1993-11-26 1994-01-12 Univ Birmingham Liquid transfer device
US5827415A (en) 1994-09-26 1998-10-27 The Board Of Trustees Of Leland Stanford Jun. Univ. Oxygen sensor
US6143248A (en) 1996-08-12 2000-11-07 Gamera Bioscience Corp. Capillary microvalve
US6090251A (en) 1997-06-06 2000-07-18 Caliper Technologies, Inc. Microfabricated structures for facilitating fluid introduction into microfluidic devices
US5992820A (en) 1997-11-19 1999-11-30 Sarnoff Corporation Flow control in microfluidics devices by controlled bubble formation
US6601613B2 (en) 1998-10-13 2003-08-05 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
US6626417B2 (en) 2001-02-23 2003-09-30 Becton, Dickinson And Company Microfluidic valve and microactuator for a microvalve
US7429354B2 (en) 2001-03-19 2008-09-30 Gyros Patent Ab Structural units that define fluidic functions
US6581438B1 (en) * 2002-01-31 2003-06-24 Sandia Corporation Capillary test specimen, system, and methods for in-situ visualization of capillary flow and fillet formation
US6561224B1 (en) 2002-02-14 2003-05-13 Abbott Laboratories Microfluidic valve and system therefor
FR2848125B1 (fr) 2002-12-04 2006-06-09 Commissariat Energie Atomique Dispositif microfluidique dans lequel l'interface liquide/fluide est stabilisee
DE10302721A1 (de) * 2003-01-23 2004-08-05 Steag Microparts Gmbh Mikrofluidische Anordnung zum Dosieren von Flüssigkeiten
EP1827693B1 (en) * 2004-12-09 2010-03-24 Scandinavian Micro Biodevices ApS A micro fluidic device and methods for producing a micro fluidic device
WO2006116616A2 (en) 2005-04-26 2006-11-02 Applera Corporation Systems and methods for multiple analyte detection
US20090282978A1 (en) 2005-07-05 2009-11-19 Massachusetts Institute Of Technology Microfluidic Separators for Multiphase Fluid-Flow Based On Membranes
US8414754B1 (en) 2006-05-31 2013-04-09 The Board Of Trustees Of The Leland Stanford Junior University Electrophoretic sample analysis and approach therefor
US7951278B2 (en) 2006-07-20 2011-05-31 The Board Of Trustees Of The Leland Standford Junior University Method of detecting directly undetectable analytes using directly detectable spacer molecules
US8562804B2 (en) 2006-07-20 2013-10-22 The Board Of Trustees Of The Leland Stanford Junior University Fluorescent finger prints for indirect detection in isotachophoresis
US8999129B2 (en) 2011-03-07 2015-04-07 Lawrence Livermore National Security, Llc Liquid and gel electrodes for transverse free flow electrophoresis
EP2139597B1 (en) 2007-04-04 2016-05-18 Micropoint Bioscience Inc. Micromachined electrowetting microfluidic valve
US8852952B2 (en) * 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
US8394251B2 (en) 2008-10-07 2013-03-12 The Board Of Trustees Of The Leland Stanford Junior University Control of chemical reactions using isotachophoresis
US8846314B2 (en) 2009-03-03 2014-09-30 The Board Of Trustees Of The Leland Stanford Junior University Isotachophoretic focusing of nucleic acids
US9285340B2 (en) 2009-08-17 2016-03-15 Lawrence Livermore National Security, Llc Methods for separating particles and/or nucleic acids using isotachophoresis
US9144799B2 (en) 2009-11-24 2015-09-29 Lawrence Livermore National Security, Llc Modular microfluidic system for biological sample preparation
US8721858B2 (en) 2010-03-12 2014-05-13 The Board Of Trustees Of The Leland Stanford Junior University Non-focusing tracers for indirect detection in electrophoretic displacement techniques
US8512538B2 (en) * 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US8986529B2 (en) 2010-09-13 2015-03-24 The Board Of Trustees Of The Leland Stanford Junior University Isotachophoresis having interacting anionic and cationic shock waves
US9151732B2 (en) 2010-10-01 2015-10-06 The Board Of Trustees Of The Leland Stanford Junior University Enhanced isotachophoresis assays using additives with spatial gradients
US8524061B2 (en) 2010-11-29 2013-09-03 The Board Of Trustees Of The Leland Stanford Junior University On-chip hybridization coupled with ITP based purification for fast sequence specific identification
GB201103917D0 (en) * 2011-03-08 2011-04-20 Univ Leiden Apparatus for and methods of processing liquids or liquid based substances
US9057673B2 (en) 2012-08-24 2015-06-16 The Board Of Trustees Of The Leland Stanford Junior University Method of preparing RNA from ribonuclease-rich sources
US20160160208A1 (en) 2013-03-14 2016-06-09 The Board Of Trustees Of The Leland Stanford Junior University Capillary barriers for staged loading of microfluidic devices
US11325123B2 (en) 2013-08-02 2022-05-10 The Board Of Trustees Of The Leland Stanford Junior University Flow regulation in fluidic systems using a phase-change material at system ports
AU2017212754B2 (en) 2016-01-29 2023-06-29 Purigen Biosystems, Inc. Isotachophoresis for purification of nucleic acids

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2304641C (en) * 1997-09-26 2003-09-02 The Regents Of The University Of Michigan Moving and mixing of microdroplets through microchannels
CN1326549A (zh) * 1998-10-13 2001-12-12 微生物系统公司 基于无源流体动力学的流体管路元件
CN1548957A (zh) * 2002-09-27 2004-11-24 ��տƼ��ɷ����޹�˾ 微流体传送及分析系统
CN1715929A (zh) * 2004-06-30 2006-01-04 生命扫描苏格兰有限公司 流控调节装置
EP1932593A1 (en) * 2006-11-22 2008-06-18 FUJIFILM Corporation Microchannel chip and converging device
EP2213364A1 (en) * 2009-01-30 2010-08-04 Albert-Ludwigs-Universität Freiburg Phase guide patterns for liquid manipulation

Also Published As

Publication number Publication date
AU2023202077A1 (en) 2023-05-04
AU2014236262A1 (en) 2015-10-15
AU2020239798A1 (en) 2020-10-29
WO2014153092A1 (en) 2014-09-25
US11851647B2 (en) 2023-12-26
AU2018206716A1 (en) 2018-08-02
US20180237767A1 (en) 2018-08-23
US10233441B2 (en) 2019-03-19
EP2972185A1 (en) 2016-01-20
GB201517364D0 (en) 2015-11-18
CA2906730A1 (en) 2014-09-25
US10787660B2 (en) 2020-09-29
US20160160208A1 (en) 2016-06-09
AU2020239798B2 (en) 2023-01-05
CA2906730C (en) 2023-03-21
US12435329B2 (en) 2025-10-07
JP2016512886A (ja) 2016-05-09
CN105190280B (zh) 2019-07-09
EP2972185A4 (en) 2017-02-22
JP6673820B2 (ja) 2020-03-25
JP2020109413A (ja) 2020-07-16
US20210062181A1 (en) 2021-03-04
CN110243637A (zh) 2019-09-17
US20240182885A1 (en) 2024-06-06
GB2526999B (en) 2020-02-19
GB2526999A (en) 2015-12-09
US20250333722A1 (en) 2025-10-30
CN105190280A (zh) 2015-12-23
AU2023202077B2 (en) 2024-09-26
US20190078077A1 (en) 2019-03-14

Similar Documents

Publication Publication Date Title
CN110243637B (zh) 用于微流体装置分段装载的毛细屏障
JP2016512886A5 (enExample)
CN101351270A (zh) 从用于芯片微流控的液柱中抽取液滴
US11911763B2 (en) Microfluidic device and methods
Yildirim et al. Phaseguides as tunable passive microvalves for liquid routing in complex microfluidic networks
JP7398469B2 (ja) 真空充填式液滴生成マイクロ流体チップおよび関連方法
JPWO2019107231A1 (ja) マイクロ流体チップ
US11590498B2 (en) Arrangement for mixing fluids in a capillary driven fluidic system
US10307756B2 (en) Capillary junction
US11110455B2 (en) Microfluidic device for electrically activated passive capillary stop valve
WO2020264447A1 (en) Open microfluidic system and various functional arrangements therefore
CN111565846A (zh) 在微流体装置中提供物质的溶液的方法
Lin Automated microfluidic device for the separation of cancer cells from blood

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant