CN110237170A - A kind of preparation method of plant cell enzymatic hydrolyzed extract that treating bird flu - Google Patents
A kind of preparation method of plant cell enzymatic hydrolyzed extract that treating bird flu Download PDFInfo
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- CN110237170A CN110237170A CN201910605588.6A CN201910605588A CN110237170A CN 110237170 A CN110237170 A CN 110237170A CN 201910605588 A CN201910605588 A CN 201910605588A CN 110237170 A CN110237170 A CN 110237170A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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Abstract
The invention discloses a kind of preparation method of plant cell enzymatic hydrolyzed extract for treating bird flu, include the following steps: to weigh fresh garlic, balsam pear is cleaned, mixing is broken into 10-20 mesh, mature vinegar is added, edible grain wine is placed in the molten device of enzymatic hydrolysis；Amyloid proteins enzyme is added in digesting molten device, high speed rotation stirs 20 minutes, daily stirring 1 time, and chitosan oligosaccharide cell fusion enzyme is added after continuously stirring 5 days, is equipped with growth accelerator mixing, stands 30 days；Under room temperature supernatant filtering, separating-purifying fused cell metabolite.One of present invention treats the preparation method of the plant cell enzymatic hydrolyzed extract of bird flu, garlic, balsam pear are pure natural medicinal and edible plant substance, the substance extracted after cell fusion digests, in addition to keeping the characteristics such as its garlic, the original immunocompetence of balsam pear, anti-inflammation and sterilization, antiviral, also there is the pharmacological property that can kill avian influenza virus, avoid the survival rate that birds are improved using side effect brought by a large amount of antibiotic and drug resistance.
Description
Technical field
The present invention relates to field of pharmaceutical technology more particularly to a kind of systems for the plant cell enzymatic hydrolyzed extract for treating bird flu
Preparation Method.
Background technique
Bird flu, full name bird class influenza are a kind of acute infectious diseases as caused by avian influenza virus, general
It is propagated between birds, rare situation can infect pig, can infect people across species barrier in rare situations.Birds infect fowl stream
Influenza Virus mainly will appear systemic or respiratory system disease, the poultry such as chicken, turkey, duck and quail and wild bird, aquatic bird, sea
The wild fowls such as bird can infect, and incidence is extremely various from acute septic death to asymptomatic band poison etc..
Currently, if it find that when birds suffer from highly pathogenic bird flu, substantially still take stringent isolation block and
The aggregate measures that full group slaughters can undoubtedly cause poultry cultivation industry huge although being able to suppress the propagation of virus in this way
Economic loss.Also there are the Chinese Traditional Medicines of prophylactic treatment bird flu currently on the market, but absorbed after taking relatively slowly, HIV suppression is made
With unobvious, therapeutic effect is unsatisfactory, in addition it is also possible to prevented and treated using a large amount of antibiotic, but its there are drugs
The shortcomings that side effect and drug resistance.
Summary of the invention
To solve to treat that slow curative effect existing for avian flu cytotoxic drug, side effect be big, skill of drug resistance in the prior art
Art problem, technical scheme is as follows:
One of present invention treats the preparation method of the plant cell enzymatic hydrolyzed extract of bird flu, includes the following steps:
Step S01, weighs fresh garlic, balsam pear is cleaned, and mixing is broken into 10-20 mesh, mature vinegar is added, edible grain wine is set
In the molten device of enzymatic hydrolysis.Wherein, mature vinegar, edible grain wine are the necessary conditions of garlic, balsam pear Plant Cytomixis, so that required work
Property substance dissolution it is more convenient.
Amyloid proteins enzyme is added in step S02 in digesting molten device, and high speed rotation stirs 20 minutes, daily stirring 1 time, even
Chitosan oligosaccharide cell fusion enzyme is added after 5 days in continuous stirring, is equipped with growth accelerator mixing, stands 30 days.Pass through mature vinegar and edible grain
The convergence media of wine acts on, and by the connection obstruction broken between cell and cell that runs at high speed, so that Garlic cell and hardship
For melon cell under the action of amyloid proteins enzyme and chitosan oligosaccharide enzyme, fusion is more extensive, and growth accelerator further increases cell fusion
Rate, and then the active material obtained is more.
Step S03, supernatant filtering under room temperature, separating-purifying fused cell metabolite.Garlic cell and balsam pear cell
Cell fusion after, the metabolite of cell is small-molecular peptides active material, with the very strong ability for killing virus, to fowl
Influenza virus is by being sensitive.
Further, in step S01, the mass concentration of mature vinegar is 30%, and the mass concentration for eating grain wine is 50%, old
Vinegar, the fluid temperature for eating grain wine are 25-30 DEG C.
Further, in step S01, garlic, mature vinegar, eats the mass ratio of grain wine as 1:1:1:1 at balsam pear.
Further, in step S02, the dispensing ratio of amyloid proteins enzyme is 10%, the dispensing ratio of chitosan oligosaccharide cell fusion enzyme
Example is 1.5%.
Further, in step S02, growth accelerator is sodium carboxymethylcellulose, and ratio of launching is 6%.
Further, it in step S03, is filtered using the filter device of 200 mesh.
The pharmacological property and effect of each raw material of the invention are as follows:
Balsam pear: balsam pear includes that quinoline abundant is peaceful and Charantin, has extremely strong adhesiveness, and have target to avian influenza virus
To adhesiveness, so that the surface protein for solidifying and changing its avian influenza virus is played, and lethal fowl stream virus.
Garlic: with the very strong ability for killing virus, to avian influenza virus by be it is sensitive, can be in very short time lethal fowl
The many types of and hypotype of influenza virus, blocks the duplication of the virus.
One of present invention treats the preparation method of the plant cell enzymatic hydrolyzed extract of bird flu, with prior art phase
Than, it has the advantage that:
One of present invention treats the preparation method of the plant cell enzymatic hydrolyzed extract of bird flu, and garlic, balsam pear are pure
Natural medicine-food homologous plant substance, the substance extracted after cell fusion digests not only have the killing virus capable of garlic,
The duplication of the virus can be blocked in many types of and hypotype of very short time lethal avian influenza virus, meanwhile, also with the adherency of balsam pear
Property, there is targeted adhesion to avian influenza virus, so that the surface protein for solidifying and changing its avian influenza virus is played, lethal fowl stream
The effect of virus, compare the prior art, can avoid making using pair brought by a large amount of antibiotic using extract of the invention
With and drug resistance, improve the survival rate of birds.
Detailed description of the invention
Fig. 1 is a kind of flow chart of the preparation method for the plant cell enzymatic hydrolyzed extract for treating bird flu in the present invention.
Specific embodiment
Below in conjunction with attached drawing of the invention, technical solution of the present invention is clearly and completely described.Based on this hair
Embodiment in bright, every other implementation obtained by those of ordinary skill in the art without making creative efforts
Example, shall fall within the protection scope of the present invention.
Embodiment 1
One of present invention treats the preparation method of the plant cell enzymatic hydrolyzed extract of bird flu, includes the following steps:
Step S01, weigh fresh garlic, balsam pear clean, mixing be broken into 10 mesh, be added equivalent mass concentration be 30%
Mature vinegar, the edible grain wine that mass concentration is 50% is placed in the molten device of enzymatic hydrolysis, wherein mature vinegar, edible grain wine temperature be
25 DEG C, garlic, balsam pear, mature vinegar, edible grain wine are that equivalent is added.
Amyloid proteins enzyme is added in step S02 in digesting molten device, and the dispensing ratio of amyloid proteins enzyme is 10%, and high speed is revolved
Turn stirring 20 minutes, daily stirring 1 time, chitosan oligosaccharide cell fusion enzyme is added after continuously stirring 5 days, chitosan oligosaccharide cell fusion enzyme
Dispensing ratio is 1.5%, and the sodium carboxymethylcellulose that dispensing ratio is 6% is added, and is stood 30 days thin to Garlic cell and balsam pear
Born of the same parents are sufficiently merged.
Step S03, supernatant filters the metabolite of 200 mesh, separating-purifying fused cell under room temperature.
Embodiment 2
One of present invention treats the preparation method of the plant cell enzymatic hydrolyzed extract of bird flu, includes the following steps:
Step S01, weigh fresh garlic, balsam pear clean, mixing be broken into 20 mesh, be added equivalent mass concentration be 30%
Mature vinegar, the edible grain wine that mass concentration is 50% is placed in the molten device of enzymatic hydrolysis, wherein mature vinegar, edible grain wine temperature be
30 DEG C, garlic, balsam pear, mature vinegar, edible grain wine are that equivalent is added.
Amyloid proteins enzyme is added in step S02 in digesting molten device, and the dispensing ratio of amyloid proteins enzyme is 10%, and high speed is revolved
Turn stirring 20 minutes, daily stirring 1 time, chitosan oligosaccharide cell fusion enzyme is added after continuously stirring 5 days, chitosan oligosaccharide cell fusion enzyme
Dispensing ratio is 1.5%, and the sodium carboxymethylcellulose that dispensing ratio is 6% is added, and is stood 30 days thin to Garlic cell and balsam pear
Born of the same parents are sufficiently merged.
Step S03, supernatant filters the metabolite of 200 mesh, separating-purifying fused cell under room temperature.
Test example:
1, test material
Subjects: Hubei Province, Tuanfeng County chicken farm, Huanggang City detects 38 bird flu positive cases.
Test drug: the metabolite of the fused cell in the embodiment of the present invention 1, antiviral agent in the prior art steam
Distilled water.
2, testing program
1. taking stringent isolation；
2. the metabolite of fused cell and water 1:3 dilution are matched and are compared to environment disinfected；
3. 38 detect that the chicken of avian influenza virus is divided into three groups, i.e., A group 20 are given the metabolite of fused cell, B group
10 are given antiviral agent, 8 distilled water of C group；
4. the chicken that 38 detect avian influenza virus is carried out oral cavity and anus stoste is perfused, 2 times a day each 1ml.
3, data processing
4, result
A group is all survived, and is got well, and start to lay eggs, and B, C group are all dead.The same period according to said method does many fowl streams
Feel positive case, all achieves satisfied effect.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (6)
1. a kind of preparation method for the plant cell enzymatic hydrolyzed extract for treating bird flu, which comprises the steps of:
Step S01, weighs fresh garlic, balsam pear is cleaned, and mixing is broken into 10-20 mesh, mature vinegar is added, edible grain wine is placed in enzyme
It solves in molten device；
Amyloid proteins enzyme is added in step S02 in digesting molten device, and high speed rotation stirs 20 minutes, and stirring 1 time, is continuously stirred daily
Chitosan oligosaccharide cell fusion enzyme is added after mixing 5 days, is equipped with growth accelerator mixing, stands 30 days；
Step S03, supernatant filtering under room temperature, separating-purifying fused cell metabolite.
2. a kind of preparation method of plant cell enzymatic hydrolyzed extract for treating bird flu according to claim 1, feature
It is, in step S01, the mass concentration of mature vinegar is 30%, and the mass concentration for eating grain wine is 50%, mature vinegar, edible grain
The fluid temperature of wine is 25-30 DEG C.
3. a kind of preparation method of plant cell enzymatic hydrolyzed extract for treating bird flu according to claim 2, feature
It is, in step S01, garlic, mature vinegar, eats the mass ratio of grain wine as 1:1:1:1 at balsam pear.
4. a kind of preparation method of plant cell enzymatic hydrolyzed extract for treating bird flu according to claim 1, feature
It is, in step S02, the dispensing ratio of amyloid proteins enzyme is 10%, and the dispensing ratio of chitosan oligosaccharide cell fusion enzyme is 1.5%.
5. a kind of preparation method of plant cell enzymatic hydrolyzed extract for treating bird flu according to claim 1, feature
It is, in step S02, growth accelerator is sodium carboxymethylcellulose, and ratio of launching is 6%.
6. a kind of preparation method of plant cell enzymatic hydrolyzed extract for treating bird flu according to claim 1, feature
It is, in step S03, is filtered using the filter device of 200 mesh.
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| CN201910605588.6A CN110237170A (en) | 2019-07-05 | 2019-07-05 | A kind of preparation method of plant cell enzymatic hydrolyzed extract that treating bird flu |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110585327A (en) * | 2019-10-10 | 2019-12-20 | 湖北慎诚生命科学有限公司 | Preparation method of plant cell zymolyte for killing and inhibiting animal parvovirus |
| CN114903955A (en) * | 2022-05-09 | 2022-08-16 | 武汉世纪迈得生命科技有限公司 | Feed additive, preparation method and application thereof in preparation of medicine for treating feline infectious peritonitis |
Citations (3)
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| CN102225146A (en) * | 2011-05-30 | 2011-10-26 | 河南黑马动物药业有限公司 | Formula and production technology of traditional Chinese medicine injection for controlling bird flu |
| CN105918821A (en) * | 2016-04-20 | 2016-09-07 | 厦门元之道生物科技有限公司 | Compound enzyme liquid having effects of lowering high blood lipids, high blood pressure and high blood sugar, and improving immunity, and preparation method thereof |
| CN107158209A (en) * | 2017-07-17 | 2017-09-15 | 孟繁华 | It is a kind of to treat herbal mixture biofermentation preparation of bird flu and preparation method thereof |
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2019
- 2019-07-05 CN CN201910605588.6A patent/CN110237170A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102225146A (en) * | 2011-05-30 | 2011-10-26 | 河南黑马动物药业有限公司 | Formula and production technology of traditional Chinese medicine injection for controlling bird flu |
| CN105918821A (en) * | 2016-04-20 | 2016-09-07 | 厦门元之道生物科技有限公司 | Compound enzyme liquid having effects of lowering high blood lipids, high blood pressure and high blood sugar, and improving immunity, and preparation method thereof |
| CN107158209A (en) * | 2017-07-17 | 2017-09-15 | 孟繁华 | It is a kind of to treat herbal mixture biofermentation preparation of bird flu and preparation method thereof |
Non-Patent Citations (2)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110585327A (en) * | 2019-10-10 | 2019-12-20 | 湖北慎诚生命科学有限公司 | Preparation method of plant cell zymolyte for killing and inhibiting animal parvovirus |
| CN114903955A (en) * | 2022-05-09 | 2022-08-16 | 武汉世纪迈得生命科技有限公司 | Feed additive, preparation method and application thereof in preparation of medicine for treating feline infectious peritonitis |
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