CN110236061A - A kind of collagen chaperonin polypeptide and the preparation method and application thereof - Google Patents
A kind of collagen chaperonin polypeptide and the preparation method and application thereof Download PDFInfo
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- CN110236061A CN110236061A CN201910563234.XA CN201910563234A CN110236061A CN 110236061 A CN110236061 A CN 110236061A CN 201910563234 A CN201910563234 A CN 201910563234A CN 110236061 A CN110236061 A CN 110236061A
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- collagen
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- collagen peptide
- chaperonin polypeptide
- chaperonin
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 144
- 108010035532 Collagen Proteins 0.000 title claims abstract description 144
- 229920001436 collagen Polymers 0.000 title claims abstract description 144
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 141
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 72
- 108050001186 Chaperonin Cpn60 Proteins 0.000 title claims abstract description 66
- 102000052603 Chaperonins Human genes 0.000 title claims abstract description 66
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 66
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 239000007787 solid Substances 0.000 claims abstract description 52
- 235000013361 beverage Nutrition 0.000 claims abstract description 36
- 239000012466 permeate Substances 0.000 claims abstract description 36
- 239000002994 raw material Substances 0.000 claims abstract description 35
- 239000012528 membrane Substances 0.000 claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000000706 filtrate Substances 0.000 claims abstract description 21
- 108010038851 tannase Proteins 0.000 claims abstract description 19
- 238000000605 extraction Methods 0.000 claims abstract description 18
- 238000001223 reverse osmosis Methods 0.000 claims abstract description 17
- 239000000919 ceramic Substances 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 16
- 108010010803 Gelatin Proteins 0.000 claims abstract description 15
- 229920000159 gelatin Polymers 0.000 claims abstract description 15
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- 235000019322 gelatine Nutrition 0.000 claims abstract description 15
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 15
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- 238000010792 warming Methods 0.000 claims abstract description 15
- 238000006243 chemical reaction Methods 0.000 claims abstract description 12
- 238000001816 cooling Methods 0.000 claims abstract description 10
- 238000001914 filtration Methods 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 7
- 238000005507 spraying Methods 0.000 claims abstract description 7
- 235000019224 Camellia sinensis var Qingmao Nutrition 0.000 claims description 20
- 235000020339 pu-erh tea Nutrition 0.000 claims description 20
- 238000012545 processing Methods 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 238000005538 encapsulation Methods 0.000 claims description 7
- 238000010348 incorporation Methods 0.000 claims description 6
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- 229910001385 heavy metal Inorganic materials 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
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- 230000008016 vaporization Effects 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
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- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 208000036119 Frailty Diseases 0.000 description 1
- 108010006519 Molecular Chaperones Proteins 0.000 description 1
- 235000019082 Osmanthus Nutrition 0.000 description 1
- 241000333181 Osmanthus Species 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000001741 anti-phlogistic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 230000000015 effect on sense Effects 0.000 description 1
- 230000003311 flocculating effect Effects 0.000 description 1
- 235000021060 food property Nutrition 0.000 description 1
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- 235000013376 functional food Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- SYUXAJSOZXEFPP-UHFFFAOYSA-N glutin Natural products COc1c(O)cc2OC(=CC(=O)c2c1O)c3ccccc3OC4OC(CO)C(O)C(O)C4O SYUXAJSOZXEFPP-UHFFFAOYSA-N 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
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- 239000004615 ingredient Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 210000000282 nail Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
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- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 229940116540 protein supplement Drugs 0.000 description 1
- 235000005974 protein supplement Nutrition 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/72—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
- A23L2/74—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration using membranes, e.g. osmosis, ultrafiltration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Water Supply & Treatment (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Non-Alcoholic Beverages (AREA)
Abstract
The present invention relates to a kind of collagen chaperonin polypeptides and the preparation method and application thereof, belong to health product technology field.The preparation method of the companion is added raw materials into warm water, and tannase or gelatin reaction are added later；After reaction, liquid is warming up to 80-90 DEG C, constant-temperature enclosed extraction, filtering, filtrate are cooled to after 50 DEG C or less again by ceramic membrane filter, are lyophilized into powder after obtained permeate natural cooling, or by obtained permeate reverse osmosis membrane be concentrated or be evaporated under reduced pressure concentration after spraying drying powder-forming to get.The collagen chaperonin polypeptide enables to the fishy smell of animal collagen peptide to be obviously blanked, and manufactured solid beverage can dissolve rapidly in warm water, and soup look is clear and transparent, and no flocculation is muddy, and the fragrance of plant is obvious, and the mouthfeel of collagen peptide is obviously improved；The trophic function of collagen peptide can not only be maintained, while being still consolidated to the trophic function of tea and flower in collagen peptide, meets the supplement for women collagen of liking to be beautiful.
Description
Technical field
The invention belongs to health product technology fields, and in particular to a kind of collagen chaperonin polypeptide and preparation method thereof with answer
With.
Background technique
Collagen peptide is to pass through enzyme hydrolysis using animal (fish scale, Animal Skin, bone) or phytoprotein as primary raw material
Or sour water solution, protein molecule be hydrolyzed to be easily soluble in water with multiple amino acids formed short peptide chains, molecular weight is no more than
2000 dalton, human body are easily digested and assimilated, and collagen and amino acid therein are to constitute human skin, bone, eyes, tooth
Tooth, tendon, internal organ, nail, the main protein of hair and amino acid are the important nutriments of human body.
Current product processing technique both domestic and external all comparative maturities, product quality is also relatively stable, and product has solid beverage
Form, also there is the product of liquid form, liquid form also sees fruity collagen peptide product.But albumen based food also have with
The edible disadvantage of plant food collocation, many plants contain tannin or are tannin, i.e., the astringent taste ingredient in plant, water-soluble
In liquid can with protein occur flocculation generate precipitating and it is muddy, influence nutritive value of food and soup look, manufacturing enterprise is in technology
Not perfect, the products application that will not attempt natural plant extraction object and collagen peptide is arranged in pairs or groups.
Collagen peptide product is from animal product such as fish, ox, pig etc., and product is after being processed into powder, many products
With animal fishy smell, only small part tasteless, but their mouthfeel is all very poor, and the intension of nutrition is also inadequate, is not able to satisfy
The color, smell, taste and shape that food needs bring the aesthetic feeling and appetite of consumer, excessively emphasize pure DEG C and function and are not concerned with the category of its food
Property, limit the development of product.
The food addition of collagen peptides at present is all that collagen is suggested by essence class additive or production and sales enterprise
After protein peptides based food brews plus honey, fruit juice are to cover fishy smell or increase flavor, can bring a kind of bad cognition of consumer,
Think that raw material is rotten or bad processing technique idea, influences consumption experience.Therefore how overcome the deficiencies in the prior art
The problem of being current health product technology field urgent need to resolve.
Summary of the invention
It is an object of the present invention to solve the deficiency of the existing technology and provide a kind of collagen chaperonin polypeptide and its preparation sides
Method and application.Collagen chaperonin polypeptide produced by the present invention enables to the fishy smell of animal collagen peptide to be obviously blanked, system
At solid beverage can be dissolved in warm water rapidly, soup look is clear and transparent, and no flocculation is muddy, and the fragrance of plant is obvious, collagen egg
The mouthfeel of white peptide is obviously improved；The trophic function of collagen peptide can not only be maintained, while also by tea and the trophic function of flower
Also it is consolidated in collagen peptide, meets the supplement for women collagen of liking to be beautiful.
To achieve the above object, The technical solution adopted by the invention is as follows:
A kind of preparation method of collagen chaperonin polypeptide, includes the following steps:
15-25kg raw material is weighed, is added in 40-50 DEG C of 400L water, and keeping temperature is to be added later single between 40-50 DEG C
Peaceful enzyme or gelatin, react 10-20 minutes；After reaction, liquid is warming up to 80-90 DEG C, keeps constant-temperature enclosed extraction at 80-90 DEG C
25-35 minutes, filtering, filtrate was cooled to after 50 DEG C or less again through ceramic membrane filter, obtained permeate；
Obtained permeate there are two types of processing mode, the two select first,
First, permeate reverse osmosis membrane is concentrated into soluble solid after obtained permeate is naturally cooled to 30 DEG C or less
Mass concentration be 15%-25%, be lyophilized into powder into freeze dryer, obtain collagen chaperonin polypeptide；
It is concentrated second, obtained permeate reverse osmosis membrane is concentrated or is evaporated under reduced pressure, the quality for being concentrated into soluble solid is dense
Degree is 15%-25%, and spraying drying powder-forming, obtains collagen chaperonin polypeptide later；
The raw material includes Pu'er tea and flower；The flower is dried flower or fresh flower；Pu'er tea weight accounts for raw material weight in raw material
The 80-90% of amount, Hua Chongliang account for the 10-20% of raw material weight；
The addition weight of tannase is 18-23g；The addition weight of gelatin is 45-55g.
It is further preferred that twice by ceramic membrane filter.
It is further preferred that the temperature that concentration is evaporated under reduced pressure is no more than 65 DEG C.
It is further preferred that filtering is filtered using 100 mesh stainless steel mesh after constant-temperature enclosed extraction；Freeze dryer freeze-drying
Operating temperature when at powder is subzero 55 DEG C.
It is further preferred that 200L water is added in the filter residue being obtained by filtration after constant-temperature enclosed extraction again, in 80-90 DEG C
Under constant-temperature enclosed progress second extraction 20-35 minutes, filtering, filtrate merge it is cooling after enter ceramic membrane together.
Present invention simultaneously provides collagen chaperonin polypeptides made from the preparation method of above-mentioned collagen chaperonin polypeptide.
The present invention also provides the applications of above-mentioned collagen chaperonin polypeptide, specifically by collagen peptide and collagen peptide companion
Companion is used in mixed way according to weight ratio 1-5:1.
The present invention additionally provides a kind of preparation methods of collagen peptide solid beverage, using above-mentioned collagen peptide companion
Companion includes the following steps:
Collagen peptide and collagen chaperonin polypeptide are mixed according to weight ratio 1-5:1 to after uniformly, control moisture content in 4%-
8%, then package encapsulation to get arrive collagen peptide solid beverage.
It is further preferred that incorporation time is 10-20min.
The present invention finally provides the drink of collagen peptide solid made from the preparation method of above-mentioned collagen peptide solid beverage
Material.
What the present invention used, which spends, to be edible flowers, but is not limited to spend, can also be with other aromatics food plant kinds.
The application method of solid beverage of the present invention are as follows: collagen peptide and collagen chaperonin polypeptide are according to weight ratio 1:1 system
At 1 gram of product can be watered 300 milliliters and brew and drink, the formula of 5:1, which can be watered 100 milliliters and brew, to be drunk, remaining ratio
Example is watered in range at this to be used in right amount, can also be adjusted according to personal mouthfeel.
Two step of preparation method part of collagen peptide solid beverage of the present invention: first is that a kind of speed is made in Pu'er tea and flower together
Molten powder --- collagen chaperonin polypeptide；Second is that instant powder is mixed assorted packaging with collagen peptide, final collagen is obtained
Peptide solid beverage product.
The present invention is reacted using 40-50 DEG C of temperature, is to be lower than this because vigor of enzyme is most strong at a temperature of this
Temperature, then vigor weakens or even debility significantly, is higher than this temperature, then enzyme inactivates；The reaction temperature of glutin is maintained at
Be conducive to flocculation reaction between 40 degree to 50 degree.Reaction time of the invention is set as 10-20 minutes, is lower than this time, then tannin
Not by fully reacting, product has astringent taste, and soup look is not clear and transparent enough.
The present invention is effectively removed heavy metal and pesticide residue by being filtered for multiple times.
The present invention decomposes the tannin in colored and Pu'er tea using tannase；It can carry out flocculating using gelatin anti-
It answers, the tannin in colored and Pu'er tea can also be removed.
The temperature setting that liquid heats up after present invention reaction is between 80-90 DEG C, it is therefore an objective to not only can be carried out and effectively mention
It takes, moreover it is possible to prevent fragrance to be lost, and carry out necessary sterilization.
The present invention is using the pesticide and heavy metal that the purpose of ceramic membrane filter is that removal is greater than 5000 dalton macromoleculars.
Those skilled in the art should know that inflated with nitrogen preservation can also be used in package encapsulation of the present invention.
Collagen chaperonin polypeptide produced by the present invention and collagen peptide have similar moisture, specific gravity, heap density, this guarantor
When having demonstrate,proved mixing, there is therebetween the good assorted uniformity.Those skilled in the art should know ratios when being used in mixed way
Example is not limited to the ratio provided in the claims in the present invention, is also adjusted according to the taste of oneself.
Present invention applicant thinks that functional food will not only bring consumer's trophic function, will also be from mouthfeel, fragrance, soup look
Consumer's aesthetic feeling for enjoying food, the assorted flower of the present invention and Special plant (Pu'er tea) are brought, food nutrition function is not only strengthened
Can, flavour of food products and aesthetic feeling are also improved, and overcome flocculation reaction of the vegatable tannin to albumen, so that animal protein and plant
Group food reaches good combination.
Compared with prior art, the present invention has the advantages that:
The collagen peptide solid beverage that collagen chaperonin polypeptide of the present invention obtains after mixing in proportion with existing collagen peptide
(those skilled in the art can also conventionally be made into liquid beverage), wherein collagen chaperonin polypeptide and collagen
The specific gravity of protein peptides, heap density, moisture are substantially close to it ensure that the mixed uniformity, product soup look is clear and transparent.Simultaneously
The chaperone of suitable tea is had found, and guarantees the limpid degree of millet paste.
The fishy smell of the application of collagen chaperonin polypeptide through the invention, animal collagen peptide is obviously blanked (without bad
Smell), the fragrance of plant is obvious, and palatability is obviously improved, and reduces heavy metal and pesticide residue；The present invention passes through technology
Tannin composition in degradation flower and Pu'er tea, effectively raises the stability after product brews；Manufactured solid beverage is in temperature
It is dissolved rapidly in water, soup look is clear and transparent, and no flocculation is muddy, and mouthfeel is pure and mild, not stimulating intestine and stomach.Product maintains collagen peptide
Trophic function, while the trophic function of tea and flower is also consolidated in collagen peptide, can meet women collagen of liking to be beautiful
Supplement, the product can also solve patients clinical and after being ill albumen supplement, Disorder of Digestion and A orption crowd nutrient protein supplement
Problem (collagen peptide of small molecule can be directly entered blood of human body without digestion), while collagen chaperonin polypeptide can also add
Strong man's body is anti-oxidant, antiphlogistic effects.
Detailed description of the invention
Fig. 1 is 1 solid beverage examining report first page of application example of the present invention；
Fig. 2 is 1 solid beverage examining report second page of application example of the present invention；
Fig. 3 is the picture of solid beverage of the present invention, collagen peptide and collagen chaperonin polypeptide of the present invention；
Fig. 4 is the picture after solid beverage of the present invention, collagen peptide and collagen chaperonin polypeptide of the present invention brew；
Fig. 5 is that comparative experiments 3 brews comparison picture；Wherein, A is comparative example 6, and B is comparative example 5, and C is test example.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and it should not be regarded as limiting this hair
Bright range.In the examples where no specific technique or condition is specified, described technology or conditions according to the literature in the art
Or it is carried out according to product description.Production firm person is not specified in material therefor or equipment, is that can be obtained by purchase
Conventional products.
Embodiment 1
A kind of preparation method of collagen chaperonin polypeptide, includes the following steps:
Weigh 15kg raw material, be added in 40 DEG C of 400L water, and keep temperature be between 40-45 DEG C be added later tannase or
Person's gelatin reacts 10 minutes；After reaction, liquid is warming up to 80-85 DEG C, keeps constant-temperature enclosed at 80-85 DEG C and extracts 25 minutes, mistake
Filter, filtrate are cooled to after 50 DEG C or less again through ceramic membrane filter, obtain permeate；
Obtained permeate there are two types of processing mode, the two select first,
First, after obtained permeate is naturally cooled to 30 DEG C or less, the concentration of permeate reverse osmosis membrane is concentrated into soluble solid
The mass concentration of shape object is 15%, is lyophilized into powder into freeze dryer, obtains collagen chaperonin polypeptide；
It is concentrated second, obtained permeate reverse osmosis membrane is concentrated or is evaporated under reduced pressure, the quality for being concentrated into soluble solid is dense
Degree is 15%, and spraying drying powder-forming, obtains collagen chaperonin polypeptide later；
The raw material includes Pu'er tea and flower；The flower be dried flower or；Pu'er tea weight accounts for raw material weight in raw material
80%, Hua Chongliang account for the 20% of raw material weight；
The addition weight of tannase is 18g；The addition weight of gelatin is 45g.
A kind of preparation method of collagen peptide solid beverage, using the present embodiment collagen chaperonin polypeptide, including it is as follows
Step:
By collagen peptide and collagen chaperonin polypeptide according to weight ratio 1:1 mixing 15min after, control moisture content in 4%-8%, so
Afterwards package encapsulation to get arrive collagen peptide solid beverage.
Embodiment 2
A kind of preparation method of collagen chaperonin polypeptide, includes the following steps:
Weigh 25kg raw material, be added in 50 DEG C of 400L water, and keep temperature be between 45-50 DEG C be added later tannase or
Person's gelatin reacts 15 minutes；After reaction, liquid is warming up to 85-90 DEG C, keeps constant-temperature enclosed at 85-90 DEG C and extracts 35 minutes, mistake
Filter, filtrate is cooled to after 50 DEG C or less is filtered by ceramic membrane twice again, obtains permeate；
Obtained permeate there are two types of processing mode, the two select first,
First, after obtained permeate is naturally cooled to 30 DEG C or less, the concentration of permeate reverse osmosis membrane is concentrated into soluble solid
The mass concentration of shape object is 25%, is lyophilized into powder into freeze dryer, obtains collagen chaperonin polypeptide；
It is concentrated second, obtained permeate reverse osmosis membrane is concentrated or is evaporated under reduced pressure, the quality for being concentrated into soluble solid is dense
Degree is 25%, and spraying drying powder-forming, obtains collagen chaperonin polypeptide later；The temperature of the reduction vaporization concentration is no more than 65
℃；
The raw material includes Pu'er tea and flower；The Hua Weizhe fresh flower；Pu'er tea weight accounts for raw material weight in raw material
90%, Hua Chongliang account for the 10% of raw material weight；
The addition weight of tannase is 23g；The addition weight of gelatin is 55g.
A kind of preparation method of collagen peptide solid beverage, using the present embodiment collagen chaperonin polypeptide, including it is as follows
Step:
By collagen peptide and collagen chaperonin polypeptide according to weight ratio 5:1 mixing 20min after, control moisture content in 4%-8%, so
Afterwards package encapsulation to get arrive collagen peptide solid beverage.
Embodiment 3
A kind of preparation method of collagen chaperonin polypeptide, includes the following steps:
Weigh 20kg raw material, be added in 45 DEG C of 400L water, and keep temperature be between 43-48 DEG C be added later tannase or
Person's gelatin reacts 20 minutes；After reaction, liquid is warming up to 84-86 DEG C, keeps constant-temperature enclosed at 84-86 DEG C and extracts 30 minutes,
The filtering of 100 mesh stainless steel mesh, filtrate is cooled to after 50 DEG C or less is filtered by ceramic membrane twice again, obtains permeate；
Obtained permeate there are two types of processing mode, the two select first,
First, after obtained permeate is naturally cooled to 30 DEG C or less, the concentration of permeate reverse osmosis membrane is concentrated into soluble solid
The mass concentration of shape object is 20%, into freeze dryer, is lyophilized into powder at subzero 55 DEG C, obtains collagen chaperonin polypeptide；
It is concentrated second, obtained permeate reverse osmosis membrane is concentrated or is evaporated under reduced pressure, the quality for being concentrated into soluble solid is dense
Degree is 20%, and spraying drying powder-forming, obtains collagen chaperonin polypeptide later；The temperature of the reduction vaporization concentration is no more than 65
℃；
The raw material includes Pu'er tea and flower；The flower is dried flower；Pu'er tea weight accounts for the 85% of raw material weight in raw material,
Flower weight accounts for the 15% of raw material weight；
The addition weight of tannase is 20g；The addition weight of gelatin is 50g.
A kind of preparation method of collagen peptide solid beverage, using the present embodiment collagen chaperonin polypeptide, including it is as follows
Step:
By collagen peptide and collagen chaperonin polypeptide according to weight ratio 1.5:1 mixing 10min after, control moisture content in 4%-8%,
Then package encapsulation to get arrive collagen peptide solid beverage.
Embodiment 4
A kind of preparation method of collagen chaperonin polypeptide, includes the following steps:
Weigh 20kg raw material, be added in 45 DEG C of 400L water, and keep temperature be between 40-50 DEG C be added later tannase or
Person's gelatin reacts 20 minutes；After reaction, liquid is warming up to 80-90 DEG C, keeps constant-temperature enclosed at 80-90 DEG C and extracts 30 minutes,
The water of 200L is added in the filtering of 100 mesh stainless steel mesh, filter residue again, the constant-temperature enclosed carry out second extraction at 80-90 DEG C, and 100
The filtering of mesh stainless steel mesh, filtrate merges and is cooled to after 50 DEG C or less is filtered by ceramic membrane twice again, obtains permeate；
Obtained permeate there are two types of processing mode, the two select first,
First, after obtained permeate is naturally cooled to 30 DEG C or less, the concentration of permeate reverse osmosis membrane is concentrated into soluble solid
The mass concentration of shape object is 20%, into freeze dryer, is lyophilized into powder at subzero 55 DEG C, obtains collagen chaperonin polypeptide；
Second, obtained permeate reverse osmosis membrane to be concentrated to or is evaporated under reduced pressure the mass concentration for being concentrated into soluble solid
It is 20%, spraying drying powder-forming, obtains collagen chaperonin polypeptide later；The temperature of the reduction vaporization concentration is no more than 65 DEG C；
The raw material includes Pu'er tea and flower；The flower is fresh flower；Pu'er tea weight accounts for the 85% of raw material weight in raw material,
Flower weight accounts for the 15% of raw material weight；
The addition weight of tannase is 20g；The addition weight of gelatin is 50g.
A kind of preparation method of collagen peptide solid beverage, using the present embodiment collagen chaperonin polypeptide, including it is as follows
Step:
By collagen peptide and collagen chaperonin polypeptide according to weight ratio 1:1 mixing 10min after, control moisture content in 4%-8%, so
Afterwards package encapsulation to get arrive collagen peptide solid beverage.
Application example 1
20 kilograms of Pu'er cooked tea, 4 kilograms of fresh rose flower are put into 500 liters of capacity Cooling or heating jars, are directly added into 40 DEG C of 400 liters of pure water,
Being heated by steam and keeping temperature is that 18 grams of tannases are added, reacts 20 minutes, is warming up to after mixing well between 40-50 DEG C
Keep allowing enzyme to inactivate and completing to extract for 25 minutes between 80-90 DEG C, filter, filter residue add 200 kg of water be warming up to 89 DEG C it is complete
At second extraction, merging filtrate is cooled to 40 DEG C hereinafter, later by filtrate by ceramic membrane filter, permeate naturally cools to 30
DEG C or less after reverse osmosis membrane concentration, the mass concentration for being concentrated into soluble solid is 15%, filtrate carry out it is cooling after enter freeze-drying
Machine is spare by that can be lyophilized within 24 hours 3 kilograms of powder or so, obtains collagen chaperonin polypeptide.3 kilograms of collagen peptides are taken, with 3
Kilogram collagen chaperonin polypeptide is sufficiently mixed by mixing machine, and incorporation time 10 minutes, mixture was wrapped by 1 gram every bag
Dress sealing, obtains collagen peptide solid beverage product.
Application example 2
20 kilograms of Pu'er cooked tea, 2 kilograms of rose dried flower are put into 500 liters of capacity Cooling or heating jars, are directly added into 40 DEG C of 400 liters of pure water,
Being heated by steam and keeping temperature is that 23 grams of tannases are added, reacts 15 minutes, is warming up to after mixing well between 40-50 DEG C
It keeps allowing enzyme to inactivate and completing to extract for 25 minutes between 80-90 DEG C, filter, filter residue adds 200 kg of water and is warming up to 85 DEG C, mentions
It takes the time 25 minutes, completes second extraction, merging filtrate is cooled to 40 DEG C hereinafter, later by filtrate by ceramic membrane filter, thoroughly
Reverse osmosis membrane concentration after liquid naturally cools to 30 DEG C or less is crossed, being concentrated into soluble solid is 25%, and filtrate cool down laggard
It is spare by the way that 2 kilograms of powder or so can be lyophilized within 24 hours to enter freeze dryer, obtains collagen chaperonin polypeptide.Take 15 kilograms of collagens
Peptide is sufficiently mixed with 3 kilograms of collagen chaperonin polypeptides by mixing machine, incorporation time 10 minutes, and mixture presses 4 grams every bag
Packaging seal is carried out, collagen peptide solid beverage product is obtained.
Application example 3
20 kilograms of Pu'er cooked tea, 2 kilograms of dried sweet-scented osmanthus are put into 500 liters of capacity Cooling or heating jars, are directly added into 40 DEG C of 400 liters of pure water, lead to
Crossing steam heating and keeping temperature is that 50 grams of gelatin are added, reacts 15 minutes after mixing well, is warming up to 80-90 between 40-50 DEG C
It is kept for 30 minutes, is filtered between DEG C, filter residue adds 200 kg of water and is warming up to 85 DEG C of progress second extractions, extracts 25 minutes, closes
And filtrate is cooled to 40 DEG C hereinafter, later by filtrate by ceramic membrane filter, permeate naturally cools to 30 DEG C or less rear reverse osmosis
Permeable membrane concentration, being concentrated into soluble solid is 20%, filtrate carry out it is cooling after enter freeze dryer and can be lyophilized into powder by 24 hours
3 kilograms or so spare, obtains collagen chaperonin polypeptide.15 kilograms of collagen peptides are taken, are passed through with 3 kilograms of collagen chaperonin polypeptides
Mixing machine is sufficiently mixed, and incorporation time 10 minutes, mixture pressed every bag of 4 grams of progress packaging seals, obtains collagen peptide
Solid beverage product.
Application example 4
20 kilograms of Pu'er cooked tea, 2 kilograms of dry chrysanthemum are put into 500 liters of capacity Cooling or heating jars, are directly added into 40 DEG C of 400 liters of pure water, lead to
Crossing steam heating and keeping temperature is that 20 grams of tannases are added, reacts 15 minutes after mixing well, is warming up to 80- between 40-50 DEG C
It keeps allowing enzyme to inactivate and completing to extract for 25 minutes between 90 DEG C, filter, filter residue adds 200 kg of water and is warming up to 89 DEG C, extracts
Time 25 minutes, second extraction is completed, merging filtrate is cooled to 40 DEG C hereinafter, later penetrating filtrate by ceramic membrane filter
Reverse osmosis membrane concentration after liquid naturally cools to 30 DEG C or less, being concentrated into soluble solid is 20%, and filtrate enters after carrying out cooling
Freeze dryer is spare by that can be lyophilized within 24 hours 3 kilograms of powder or so, obtains collagen chaperonin polypeptide.3 kilograms of collagen peptides are taken,
Be sufficiently mixed with 1 kilogram of collagen chaperonin polypeptide by mixing machine, incorporation time 10 minutes, mixture by 4 grams every bag into
Row packaging seal obtains collagen peptide solid beverage product.
Collagen chaperonin polypeptide moisture content 5% or so made from above-mentioned 4 application examples has good dissolubility, fragrant
Gas is consistent with this flower smell that its raw material uses, and is mixed in proportion with collagen peptide.
Comparative experiments 1
Test example are as follows: the method that tannase is added in embodiment 4, permeate processing mode select the first, Hua Caiyong rose dried flower；
Comparative example 1 and the difference of test example are: Extracting temperature is fluidized state, remaining is all identical；
The difference of comparative example 2 and test example is: Extracting temperature is between 40-50 DEG C, remaining is all identical；
Experimental result: for 1 product of comparative example compared with 4 product of embodiment, fragrance is thin, and high temperature extraction causes fragrance matter to be lost；
2 product of comparative example is compared with 4 product of embodiment, fragrance no significant difference, but the amount of obtained collagen chaperonin polypeptide is reduced
28%.
Comparative experiments 2
Test example are as follows: the method that tannase is added in embodiment 4, permeate processing mode select the first, Hua Caiyong rose dried flower；
Comparative example 3 and the difference of test example are: extraction time 10min, remaining is all identical；
Comparative example 4 and the difference of test example are: extraction time 60min, remaining is all identical；
Experimental result: for 3 product of comparative example compared with 4 product of embodiment, the amount of obtained collagen chaperonin polypeptide reduces 35%；
4 product of comparative example is compared with 4 product of embodiment, the amount no significant difference of obtained collagen chaperonin polypeptide, but collagen
Polyphenol content is substantially reduced in chaperonin polypeptide, reduces 31%.
Comparative experiments 3
Test example are as follows: the method that tannase is added in embodiment 4, permeate processing mode select the first, Hua Caiyong rose dried flower；
Collagen peptide solid beverage 0.2 is brewed using 60mL water；
Comparative example 5 and the difference of test example are: being added without tannase, remaining is all identical；
Comparative example 6 is after Pu'er tea, rose dried flower are directly impregnated 15min, to take liquid that collagen peptide is added；Pu'er tea, rose
The dosage of rare dried flower is identical to be converted to Pu'er tea, rose dried flower raw material dosage in test example collagen peptide solid beverage
Amount；The amount of collagen peptide contained in the dosage of collagen peptide and test example collagen peptide solid beverage is identical.
Experimental result, as shown in figure 5, test example soup look is clear and transparent, no flocculation is muddy, and mouthfeel is excellent, there is Rose Essentielle,
Without bitter taste, alcohol thickness is preferable.5 soup look of comparative example has certain flocculation muddy, mouthfeel is bad, there is hardship compared with test example soup look weight
Astringent taste, alcohol thickness is general, can smell Rose Essentielle；Comparative example 6 is in completely cloudy state, has precipitating, mouthfeel is poor, bitter taste
Weight, has slightly fishy smell (slightly light compared with collagen peptide fishy smell), and bitter taste masks Rose Essentielle.
In addition, the tea cream that the present invention additionally uses conventional method preparation is mixed with collagen peptide and is brewed, as a result, it has been found that, by
It cannot be merged well in tea cream and powdered collagen peptide, and have flocculation sedimentation, largely effect on sense organ.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (10)
1. a kind of preparation method of collagen chaperonin polypeptide, which comprises the steps of:
15-25kg raw material is weighed, is added in 40-50 DEG C of 400L water, and keeping temperature is to be added later single between 40-50 DEG C
Peaceful enzyme or gelatin, react 10-20 minutes；After reaction, liquid is warming up to 80-90 DEG C, keeps constant-temperature enclosed extraction at 80-90 DEG C
25-35 minutes, filtering, filtrate was cooled to after 50 DEG C or less again through ceramic membrane filter, obtained permeate；
Obtained permeate there are two types of processing mode, the two select first,
First, being concentrated into soluble solid by reverse osmosis membrane after obtained permeate is naturally cooled to 30 DEG C or less
Mass concentration is 15%-25%, is lyophilized into powder into freeze dryer, obtains collagen chaperonin polypeptide；
It is concentrated second, obtained permeate reverse osmosis membrane is concentrated or is evaporated under reduced pressure, the quality for being concentrated into soluble solid is dense
Degree is 15%-25%, and spraying drying powder-forming, obtains collagen chaperonin polypeptide later；
The raw material includes Pu'er tea and flower；The flower is dried flower or fresh flower；Pu'er tea weight accounts for raw material weight in raw material
The 80-90% of amount, Hua Chongliang account for the 10-20% of raw material weight；
The addition weight of tannase is 18-23g；The addition weight of gelatin is 45-55g.
2. the preparation method of collagen chaperonin polypeptide according to claim 1, which is characterized in that pass through ceramic membrane filter two
It is secondary.
3. the preparation method of collagen chaperonin polypeptide according to claim 1, which is characterized in that the temperature of concentration is evaporated under reduced pressure
Degree is no more than 65 DEG C.
4. the preparation method of collagen chaperonin polypeptide according to claim 1, which is characterized in that mistake after constant-temperature enclosed extraction
Filter is filtered using 100 mesh stainless steel mesh；Operating temperature when freeze dryer is lyophilized into powder is subzero 55 DEG C.
5. the preparation method of collagen chaperonin polypeptide according to claim 1, which is characterized in that mistake after constant-temperature enclosed extraction
It filters obtained filter residue and 200L water is added again, constant-temperature enclosed progress second extraction 20-35 minutes at 80-90 DEG C, filtering, filtrate
Enter ceramic membrane together after merging cooling.
6. collagen peptide companion made from the preparation method using collagen chaperonin polypeptide described in claim 1-5 any one
Companion.
7. the application of collagen chaperonin polypeptide as claimed in claim 6, which is characterized in that by collagen peptide and collagen peptide
Companion is used in mixed way according to weight ratio 1-5:1.
8. a kind of preparation method of collagen peptide solid beverage, special using collagen chaperonin polypeptide as claimed in claim 6
Sign is, includes the following steps:
It will mix to after uniformly, control moisture content is in 4%-8%, and then package encapsulation is to get arriving collagen peptide solid beverage.
9. the preparation method of collagen peptide solid beverage according to claim 8, which is characterized in that incorporation time is
10-20min。
10. collagen peptide made from the preparation method of collagen peptide solid beverage described in claim 8-9 any one
Solid beverage.
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| CN112970891A (en) * | 2021-04-01 | 2021-06-18 | 贝因美（杭州）食品研究院有限公司 | Instant tea milk solid beverage and preparation method thereof |
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