CN110229762A - One plant has the hydrogen-oxidizing bacterium of Plant growth promotion and its is separately cultured and applies - Google Patents
One plant has the hydrogen-oxidizing bacterium of Plant growth promotion and its is separately cultured and applies Download PDFInfo
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- CN110229762A CN110229762A CN201910158266.1A CN201910158266A CN110229762A CN 110229762 A CN110229762 A CN 110229762A CN 201910158266 A CN201910158266 A CN 201910158266A CN 110229762 A CN110229762 A CN 110229762A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The invention discloses one plant of Microbacterium Microbacterium1-0-19, which is classified as Microbacterium bacterium near the grade separation of Shaanxi Province, Tongchuan, Tongchuan City Yaozhou District, based on physio-biochemical characteristics and 16SrDNA analysis.The present invention separates hydrogen-oxidizing bacterium using hydrogen production device is persistently led to using alfalfa rhizosphere soil as research object；Its classification position is determined by morphologic observation to hydrogen-oxidizing bacterium and Physiology and biochemistry, 16SrDNA identification.The present invention, in laboratory ferment, prepares hydrogen bacteria preparation with optimal bacterial strain, which can be used for agricultural production and promote plant growth, improve yield, improves fertilizer efficiency and improves the anti-adversity ability of plant.
Description
Technical field
The present invention relates to the separation method of hydrogen-oxidizing bacterium 1-0-19 and applications, and in particular to a kind of without the beans for inhaling hydrogen enzyme
The hydrogen-oxidizing bacterium Microbacterium 1-0-19(Microbacterium sp. 1-0- being separately cultured out in section's plant rhizosphere soil
19) and its microbial bacterial agent, hydrogen-oxidizing bacterium 1-0-19 bacterial strain in 2018 September 19 be preserved in Wuhan University's Chinese Typical Representative
Culture collection, deposit number are CCTCC No:M 2018638.
Background technique
During permanent agro-farming, it has been found that make the rule that can increase production between legume crop rotation, but all right
Its physiology course and mechanism, which are done, deeply probes into.In earlier 1900s, Dong Zhongmin professor et al. passes through experiment, it was found that leguminous plant
Root nodule has H during fixed nitrogen2It releases, but the content detected in the soil is seldom even without it is inferred that this
The phenomenon that hydrogen disappears may be related with quasi-microorganism a certain or a certain in soil.By a large amount of experimental verification, it was found that
One kind can aoxidize the bacterium of hydrogen, be referred to as hydrogen-oxidizing bacterium.
Hydrogen-oxidizing bacterium belongs to plant growth-promoting rhizobacteria, and the growth of it and crops and environment remediation have very close pass
, especially there is significant facilitation in system to crop yield, therefore have extensive practical significance for the research of hydrogen-oxidizing bacterium
With value.The 19th-century later period, foreign countries just had begun the research to hydrogen-oxidizing bacterium, so for point of hydrogen-oxidizing bacterium
Tend to be mature from method and means, but in general, the research of hydrogen-oxidizing bacterium is especially deep not yet, still in first
The grade stage.Currently, scientist studies the pedotheque in some regions of the country such as Canada, soil-like is found
Product pass through H2After processing, the plant growth degree of progress speed of plantation can be made, it was confirmed that " hydrogen fertilizer is theoretical ".
The H discharged during legume symbiosis fixed nitrogen2And its depend on H2And the hydrogen-oxidizing bacterium grown constitutes pulse family
Biological community structure specific to plant rhizosphere, and to plant rhizosphere soil microorganism and plant pathogenic microorganisms ecosystem
System generates beneficial effect.The research of concern legume symbiosis fixed nitrogen hydrogen release will enrich the content of biological nitrogen fixation, and to soil
Facilitation is played in the research of earth fertility and Nutrient Cycling.
The separation method of new hydrogen-oxidizing bacterium is explored in the research for carrying out legume rhizosphere hydrogen-oxidizing bacterium, is obtained new
Physiological-type microbial strains, study the physiological and ecological characteristic and physiological activator of its population, facilitate abundant rhizosphere
Microbial resources disclose molecular basis relevant to the crop rotation of legume, Intercropping Benefit, mention for developing agricultural sustainable development
For new approaches.
Summary of the invention
The object of the present invention is to provide one plant of hydrogen-oxidizing bacterium Microbacterium 1-0-19(Microbacterium sp. 1-
0-19) and its microbial bacterial agent.
The present invention realizes process:
A kind of hydrogen-oxidizing bacterium 1-0-19, classification naming are hydrogen-oxidizing bacterium Microbacterium 1-0-19(Microbacterium
Sp. 1-0-19), on September 19th, 2018 by China typical culture collection center preservation, deposit number is CCTCC No:
M 2018638.Its Ecological Characteristics is as follows: spherical, small, white, neat in edge is relatively wet, Gram-positive.
The preparation method of above-mentioned hydrogen-oxidizing bacterium 1-0-19, comprising the following steps:
(1) sample acquires: acquisition growth is vigorous and without the dross leguminous plant rhizosphere soil for inhaling hydrogen enzyme according to a conventional method；
(2) soil enrichment: the pedotheque of acquisition is placed in and persistently leads to H2Culture apparatus, the hydroxide in Enriching soil is thin
Bacterium；
(3) strain separating and purifying: soil dilution is coated on mineral salt culture medium plate, is placed in closed container, and room temperature is inverted training
It supports, grows obvious bacterium colony to plate, picking single colonie is further purified with method of dilution butteron on plate；
(4) bacterial screening: the identification of hydrogenase activity measurement and bacterial strain autophyting ability.
The preparation method of above-mentioned hydrogen-oxidizing bacterium 1-0-19: wherein without the leguminous plant for inhaling hydrogen enzyme described in step (1)
Including alfalfa, soybean, Chinese milk vetch or clover, rhizosphere soil range is within root nodule 5mm.
In above-mentioned steps (2), soil supension is made in the rhizosphere soil of the acquisition, by soil supension on superclean bench
The dilution of each gradient is uniformly coated on MSA mineral salt plating medium by continuous gradient dilutions, and inversion is incubated at gas
Body circulation system is cultivated three weeks under room temperature, observes the growing state of bacterium on culture medium, and picking size and form color is different
Bacterium colony, be crossed on mineral salt culture medium and continuously cultivate, the purifying of each bacterium colony more than three times, obtains initial hydrogen-oxidizing bacterium
Doubtful bacterial strain is transferred on beef extract-peptone test tube slant, and 4 DEG C of preservations are spare.
Above-mentioned hydrogen-oxidizing bacterium is promoting the application in plant growth.
Solid fungicide containing above-mentioned hydrogen-oxidizing bacterium, preparation method are as follows: by fresh hydrogen-oxidizing bacterium fermentation liquid with
Turf, peat soil, rice bran, vegetable garden soil, wood sawdust or montmorillonite are that solid fungicide is made in adsorbent.
It is attached that Microbacterium Microbacterium1-0-19 of the present invention is located away from Shaanxi Province, Tongchuan, Tongchuan City Yaozhou District grade separation
Closely, alfalfa crop field is classified as Microbacterium bacterium based on physio-biochemical characteristics and 16SrDNA analysis.The present invention is with pale reddish brown
Alfalfa Rhizospheric Soils are research object, are separated using hydrogen production device is persistently led to hydrogen-oxidizing bacterium；By to hydrogen-oxidizing bacterium
Morphologic observation and Physiology and biochemistry, 16SrDNA identification determine its classification position, it was found that one plant of hydrogen-oxidizing bacterium novel bacterial,
The hydrogen-oxidizing bacterium can promote plant growth, for improvement soil property and biological nitrogen fixation resource development and utilization provide science according to
According to providing fundamental basis for agricultural research.The present invention, in laboratory ferment, prepares hydrogen bacteria preparation with optimal bacterial strain.The biology
Plant growth can be improved for agricultural production in preparation, improves yield, improves fertilizer efficiency and improves the anti-adversity ability of plant.
Detailed description of the invention
Fig. 1 is the bacterial strain influence long to wheat seed root long and bud；
Fig. 2 is influence of the bacterial strain to wheat germination fresh weight and dry weight.
Specific embodiment
Hydrogen-oxidizing bacterium 1-0-19 of the invention is the gram-positive bacterium cultivated in 28 DEG C of MSA culture medium.
The solid medium group is divided into MSA culture medium: (NH4)2SO4 5.00g, NaCl 5.00g, K2HPO4
2.00g, KH2PO4 5.00g,CaCl2 0.01g, MgSO4 0.20g, FeSO4·7H2O 0.01g, sterile soil dilution
(it is diluted to 10-4) 100mL, water 900mL, pH 7.2.
Its identification mark is as follows:
1, physiological and biochemical property
The physiological and biochemical property of 1-0-19 bacterial strain is shown in table 1, and the 1-0-19 bacterial strain is in spherical, Gram-positive,
Bacterium colony can be formed on MSA culture medium, bacterium colony is small, and white edge is neat, relatively wet.
Note: "+" indicates positive, and "-" indicates negative.
1: Starch Hydrolysis；2: methyl red；3:V-P measurement；4；Cellulose decomposition；5: nitrate reduction；6: nitrous acid reduction；
7: producing ammonia test；8: glucose oxidative fermentation；9: catalase；10: oxidizing ferment；11: nitrogenase activity；12: indoles；13: gelatin
Liquefaction；14: acetic acid oxidation.
2, sequence is analyzed
Bacterial strain is sent to Xi'an and holds up Ke Zexi Bioisystech Co., Ltd progress sequencing analysis, strain idenfication.
Strain idenfication result: the strain sequence that sequencing obtains is subjected to Blast sequence alignment on NCBI, comparison result is aobvious
Show, the sequence and Microbacterium sequence similarity of bacterial strain 1-0-19 is 100%, can determine that bacterial strain 1-0-19 exists accordingly
Belong to Microbacterium on Molecular Phylogeny taxology.
The isolated culture method of hydrogen-oxidizing bacterium of the invention is to utilize persistently logical H first2Culture apparatus realize to soil
The enrichment of earth sample, then by its dilution spread in MSA culture medium flat plate, screening can be with H2For the energy and assimilate CO2It is unique
The bacterial strain of carbon source, and then purify and MSA medium slant of transferring, then carry out the identification of hydrogenase and bacterial strain autophyting ability, thus
Bacterium.The ultimate analysis bacterial strain is promoting the effect in plant growth.
Embodiment 1: edaphon 1-0-19's is separately cultured
1, sample acquires: the pedotheque acquired respectively in May, 2016, July, September to Shaanxi Province Tongchuan City Yaozhou District utilizes
" S-shaped sampling method " selects several sampled points in big Tanaka, removes topsoil and the residual branch of leaf, excavate to upper soll layer l0cm with
Under, the soil around rhizosphere of alfalfa 5-15cm is collected with hermetic bag, mixes soil sample after the completion of acquisition, the soil sample after mixing point
At three parts, it is spare that -80 DEG C of Storage in refrigerator are put into as three repetitions, after liquid nitrogen flash freezer.
Nearby big field acquires the Rhizosphere Soil of non-leguminous plant according to the above method, as control.
2, soil enrichment with separate
Clover soil sample 5.0g is accurately weighed respectively, is put into the conical flask equipped with 45mL sterile water, and 30 small glass are housed in bottle
Pearl helps to be completely mixed soil sample uniformly.Conical flask is put on 180r/min shaking table and is shaken 30 minutes, the soil formed in this way
Earth suspension is the Initial dilution concentration 10 of soil-1, by soil supension continuous gradient dilutions to 10 on superclean bench-10, will be each
The dilution of gradient is all uniformly coated on MSA mineral salt plating medium, and inversion is incubated at gas circulation system, each gradient
Three parallel.
Non- pulse family soil sample is also diluted coating according to the above method and cultivates.It cultivates three weeks under room temperature, observation culture
The growing state of bacterium on base, the different bacterium colony of picking size and form color are crossed on mineral salt culture medium and continuously cultivate, often
A bacterium colony purifying more than three times, obtains the doubtful bacterial strain of initial hydrogen-oxidizing bacterium, it is oblique to be transferred to beef extract-peptone test tube
On face, 4 DEG C of preservations are spare.
3, bacterial screening
Screening the common experimental method of the bacterium containing hydrogenase is TTC(2,3,5- benzyltriphenylphosphonium chloride tetrazole) method.It will be sterilized
The filter membrane of bacterium is placed on MSA solid medium, and bacterial strain point to be detected is connected on filter membrane, gas circulation system is placed into
In, to filter membrane on when growing obvious bacterium colony, filter membrane is taken out.Place it in the filter that newly prepare 0.1% TTC solution was impregnated with
It is dark in air at room temperature to place 10-20min on paper, pay attention to observing color change and intensity that bacterium colony is likely to occur, then
It is placed in the closed container for being passed through hydrogen again, room temperature dark places 10-20min, pays attention to the color change of bacterium colony.Face in air
Color does not change and the bacterial strain for the color that reddens under hydrogen atmosphere has hydrogenase activity for the positive, the as bacterium of containing hydrogenated enzyme, often
A bacterial strain repeats three times, to ensure the accuracy of experimental result.
It identifies whether strain to be tested can be carried out autophyting growth as unique energy source using hydrogen, is the stringent detection of comparison
The method of hydrogen-oxidizing bacterium.The test of bacterial strain autophyting growth includes two methods of plate culture and shaking flask culture.
Plate cultural method: taking the higher several plants of bacterium bacterial strains of hydrogenase activity, prepares bacteria suspension, successively gradient is dilute respectively
Release (diluted concentration 10-1, 10-2, 10-3, 10-4, 10-5, 10-6), it is 10 by dilution-4, 10-5, 10-6Bacterium solution be applied to MSA
Mineral salt culture medium, the culture medium of sterile water coating is as blank control.Plate is inverted in gas-cycle incubation system, is made
The flow velocity of gas is 280mL/min in device, and the content of hydrogen is 832ppm, and continuous culture two weeks pays attention to observing on culture medium
The growing state of bacterium colony.Another group of culture medium is placed directly in air, at room temperature continuous culture two weeks, and observation was cultivated
The growing state of bacterium colony in journey, judges whether bacterial strain can carry out autophyting growth using hydrogen as unique energy source.
Shaking table culture method: strain to be tested is inoculated into MSA fluid nutrient medium, and two groups of experiments is divided to carry out.One group of taper
Bottle is closed with the sealing of sterile rubber stopper, and air inlet pipe and an air outlet pipe is housed on rubber stopper.It is 280mL/min with flow velocity, hydrogen contains
Amount is the mixed gas of 832ppm by the gas displacement in conical flask, stoppers nozzle, shakes on 30 DEG C, the shaking table of 120r/min
Culture, it is every two days that gas displacement in conical flask is primary, it cultivates one week, obstructed hydrogen in another group of conical flask, at 30 DEG C,
Aerobic culture on the shaking table of 120r/min.Bacteria suspension is sampled daily, with 721 spectrophotometers in 560nm wavelength condition
Under, the OD value of bacteria suspension is measured, influence of the hydrogen to strain growth is detected.
It is detected by hydrogenase activity and autophyting ability, filters out 16 plants of hydrogen-oxidizing bacteriums, including bacterial strain 1-0-19, bacterial strain
1-1-2, bacterial strain 1-2-3, bacterial strain 1-2-9, bacterial strain 1-3-2, bacterial strain 2-0-4, bacterial strain 2-1-4, bacterial strain 2-2-16, bacterial strain 2-3-1,
Bacterial strain 2-3-6, bacterial strain 2-3-8, bacterial strain 3-0-15, bacterial strain 3-1-1, bacterial strain 3-1-17, bacterial strain 3-3-7, bacterial strain 3-3-10.These
Bacterial strain has stronger hydrogenase activity, and in the case where only hydrogen is as the energy, can in plate and conical flask
Autophyting growth is carried out, it is determined that these bacterial strains are hydrogen-oxidizing bacterium.
The plant growth of 2 hydrogen-oxidizing bacterium 1-0-19 of embodiment promotes effect
Full no insect pest is chosen, wheat seed similar in size is put into the water of 5 times of grain weights, is gently mixed the left side 15min
It is right.Then, with 30 DEG C of warm water immersion 6h, (soaking time is suitable, if the time is too long, seed will will do it anaerobic respiration, generation
Ethyl alcohol can damage seed, reduce germination percentage;If the time is too short, mortifier can not be leached in seed), seed vitality at this temperature
Highest.Again with 75% alcohol treatment 1min, then with aseptic water washing remove ethyl alcohol, then with sodium hypochlorite impregnate 5min, with nothing
Bacterium water is repeatedly rinsed well, the wheat seed of disinfection is placed in sterile culture dish and (is covered with sterilized filter in culture dish
Paper), 15 are put in each culture dish, cover completely wet gauze, 27 DEG C of dark place vernalization 2d above.Bacterial strain to be measured is connected to
In LB liquid medium, 27 DEG C, shake culture 48h on 180r/min shaking table selects the almost the same wheat of Germinating status and exists
3h is placed in 15mL bacteria suspension, continues illumination cultivation l0d on plate, the brightness time is assigned as 1:1, observes the growth feelings of wheat
Condition, root long, the bud for recording wheat after 10d be long, fresh weight and dry weight.As the result is shown in table 2.
Note: numerical value is 3 duplicate average values in figure, and " scholar " subsequent numerical value is standard deviation.
Claims (9)
1. a kind of hydrogen-oxidizing bacterium 1-0-19, classification naming is hydrogen-oxidizing bacterium Microbacterium 1-0-19
(Microbacterium sp. 1-0-19) was protected on September 19th, 2018 by China typical culture collection center preservation
Hiding number is CCTCC No:M 2018638.
2. hydrogen-oxidizing bacterium 1-0-19 described in claim 1, Ecological Characteristics are as follows: spherical, small, white, neat in edge,
It is relatively wet, Gram-positive.
3. the preparation method of hydrogen-oxidizing bacterium 1-0-19 described in claim 1, comprising the following steps:
(1) sample acquires: acquisition growth is vigorous and without the dross leguminous plant rhizosphere soil for inhaling hydrogen enzyme according to a conventional method；
(2) soil enrichment: the pedotheque of acquisition is placed in and persistently leads to H2Culture apparatus, the hydrogen-oxidizing bacterium in Enriching soil；
(3) strain separating and purifying: soil dilution is coated on mineral salt culture medium plate, is placed in closed container, and room temperature is inverted training
It supports, grows obvious bacterium colony to plate, picking single colonie is further purified with method of dilution butteron on plate；
(4) bacterial screening: the identification of hydrogenase activity measurement and bacterial strain autophyting ability.
4. the preparation method of hydrogen-oxidizing bacterium according to claim 3, it is characterised in that: wherein described in step (1) not
It include alfalfa, soybean, Chinese milk vetch or clover containing the leguminous plant for inhaling hydrogen enzyme, rhizosphere soil range is apart from root nodule 5mm
Within.
5. the preparation method of hydrogen-oxidizing bacterium according to claim 3, it is characterised in that: wherein step (2) acquisition
Rhizosphere soil soil supension is made, by soil supension continuous gradient dilutions on superclean bench, by the dilution of each gradient
Liquid is all uniformly coated on MSA mineral salt plating medium, and inversion is incubated at gas circulation system, cultivates three under room temperature
In week, the growing state of bacterium on culture medium is observed, the different bacterium colony of picking size and form color is crossed on mineral salt culture medium
Continuous culture, each bacterium colony purifying more than three times, obtain the doubtful bacterial strain of initial hydrogen-oxidizing bacterium, are transferred to beef extract egg
On white peptone test tube slant, 4 DEG C of preservations are spare.
6. the preparation method of hydrogen-oxidizing bacterium according to claim 5, it is characterised in that the MSA mineral salt plate training
Support base composition are as follows: (NH4)2SO4 5.00g, NaCl 5.00g, K2HPO4 2.00g, KH2PO4 5.00g,CaCl2 0.01g,
MgSO4 0.20g, FeSO4·7H2O 0.01g, sterile soil dilution 100mL, water 900mL, pH 7.2.
7. hydrogen-oxidizing bacterium described in claim 1 is promoting the application in plant growth.
8. containing the solid fungicide of hydrogen-oxidizing bacterium described in claim 1.
9. the preparation method of hydrogen-oxidizing bacterium solid fungicide according to any one of claims 8, it is characterised in that: fresh hydroxide is thin
Solid fungicide is made using turf, peat soil, rice bran, vegetable garden soil, wood sawdust or montmorillonite as adsorbent in fermented liquid.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521323A (en) * | 2019-09-29 | 2019-12-03 | 南京林业大学 | A method of locust tree of sowing grass seeds by duster on the exposed palisades of shale is efficiently replied immediately green fastly and improves soil pH value |
| CN112300958A (en) * | 2020-08-04 | 2021-02-02 | 西安医学院 | Hydroxide bacteria S-1-4 and screening method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102268391A (en) * | 2011-07-09 | 2011-12-07 | 西北大学 | Hydrogen-oxidizing bacteria WMQ-7, and separation method and application thereof |
| CN102603371A (en) * | 2012-03-19 | 2012-07-25 | 西安文理学院 | Microbial fertilizer and preparation method thereof |
| JP2015171340A (en) * | 2014-03-12 | 2015-10-01 | 東ソー株式会社 | Recombinant hydrogen oxidizing bacteria and processes of protein production using the same |
| CN105431538A (en) * | 2013-06-14 | 2016-03-23 | 赢创德固赛有限公司 | Method for producing organic compositions from oxyhydrogen and CO2 via acetoacetyl-coa as intermediate product |
| JP2016185088A (en) * | 2015-03-27 | 2016-10-27 | 東ソー株式会社 | Recombinant hydrogen oxidizing bacteria and methods for producing proteins using the same |
| CN106164268A (en) * | 2013-09-03 | 2016-11-23 | 贝尔生物系统公司 | The host cell utilizing artificial endosymbiont is modified |
| CN107760622A (en) * | 2017-11-03 | 2018-03-06 | 深圳克格瑞环保生物科技有限公司 | One strain denitrogen paracoccus and the method for high ammonia-nitrogen wastewater processing production single cell protein |
| CN109880757A (en) * | 2019-03-04 | 2019-06-14 | 西北大学 | One plant of hydrogen-oxidizing bacterium and its separation method and application with itself nitrogen fixing capacity |
-
2019
- 2019-03-04 CN CN201910158266.1A patent/CN110229762A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102268391A (en) * | 2011-07-09 | 2011-12-07 | 西北大学 | Hydrogen-oxidizing bacteria WMQ-7, and separation method and application thereof |
| CN102603371A (en) * | 2012-03-19 | 2012-07-25 | 西安文理学院 | Microbial fertilizer and preparation method thereof |
| CN105431538A (en) * | 2013-06-14 | 2016-03-23 | 赢创德固赛有限公司 | Method for producing organic compositions from oxyhydrogen and CO2 via acetoacetyl-coa as intermediate product |
| CN106164268A (en) * | 2013-09-03 | 2016-11-23 | 贝尔生物系统公司 | The host cell utilizing artificial endosymbiont is modified |
| JP2015171340A (en) * | 2014-03-12 | 2015-10-01 | 東ソー株式会社 | Recombinant hydrogen oxidizing bacteria and processes of protein production using the same |
| JP2016185088A (en) * | 2015-03-27 | 2016-10-27 | 東ソー株式会社 | Recombinant hydrogen oxidizing bacteria and methods for producing proteins using the same |
| CN107760622A (en) * | 2017-11-03 | 2018-03-06 | 深圳克格瑞环保生物科技有限公司 | One strain denitrogen paracoccus and the method for high ammonia-nitrogen wastewater processing production single cell protein |
| CN109880757A (en) * | 2019-03-04 | 2019-06-14 | 西北大学 | One plant of hydrogen-oxidizing bacterium and its separation method and application with itself nitrogen fixing capacity |
Non-Patent Citations (4)
| Title |
|---|
| JIAN YU: "Fixation of carbon dioxide by a hydrogen-oxidizing bacterium for value-added products", 《WORLD J MICROBIOL BIOTECHNOL》 * |
| 常小娟: "紫花苜蓿根际氢氧化细菌的分离鉴定及氢气对根际微生物多样性的影响", 《中国优秀硕士学位论文全文数据库（电子期刊）农业科技辑》 * |
| 李忠玲等: "1株具促生作用的氢氧化细菌的分离及鉴定", 《江苏农业科学》 * |
| 蒙渊等: "氢氧化细菌分离、筛选及促生机制研究进展 ", 《微生物学通报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521323A (en) * | 2019-09-29 | 2019-12-03 | 南京林业大学 | A method of locust tree of sowing grass seeds by duster on the exposed palisades of shale is efficiently replied immediately green fastly and improves soil pH value |
| CN110521323B (en) * | 2019-09-29 | 2022-02-01 | 南京林业大学 | Method for efficiently and quickly spraying locust trees on exposed shale rock wall to restore green and improve soil pH value |
| CN112300958A (en) * | 2020-08-04 | 2021-02-02 | 西安医学院 | Hydroxide bacteria S-1-4 and screening method thereof |
| CN112300958B (en) * | 2020-08-04 | 2023-12-05 | 西安医学院 | Hydroxyl bacteria S-1-4 and screening method thereof |
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