CN110229213A - A kind of artificial synthesized small-molecular peptides, growth regulator and its application method containing the artificial synthesized micromolecule peptide - Google Patents
A kind of artificial synthesized small-molecular peptides, growth regulator and its application method containing the artificial synthesized micromolecule peptide Download PDFInfo
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- CN110229213A CN110229213A CN201910401957.XA CN201910401957A CN110229213A CN 110229213 A CN110229213 A CN 110229213A CN 201910401957 A CN201910401957 A CN 201910401957A CN 110229213 A CN110229213 A CN 110229213A
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- artificial synthesized
- molecular peptides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
- A01H6/201—Brassica juncea
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
- A01H6/202—Brassica napus [canola]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
Abstract
The invention discloses a kind of artificial synthesized small-molecular peptides, growth regulator and its application method containing the artificial synthesized micromolecule peptide, and the amino acid sequence of the small-molecular peptides is as shown in Seq ID No.1.The growth regulator is the growth regulator for promoting plant absorption mineral element.The mineral element includes at least one of Mg, Ca, Mn, Fe, Zn, Cu or B.The application method is applied on the root or blade of plant the following steps are included: the artificial synthesized small-molecular peptides are configured to solution.The small-molecular peptides structure of the present invention program is simple, is easily absorbed and used by plants, and can not only be utilized by the root absorption of plant, can also transmit signal to root after being absorbed by blade so as to cause the variation of microelement in root；The peptide only needs micro addition that can play significant facilitation to the absorption process of a variety of mineral elements, meanwhile, which also has certain facilitation to plant growth.
Description
Technical field
The present invention relates to field of biotechnology, and in particular to a kind of artificial synthesized small-molecular peptides, artificial synthesized small containing this
The growth regulator and its application method of molecular peptide.
Background technique
Polypeptide is a-amino acid with the compound that peptide bond links together and is formed, and is the intermediate product of protein hydrolysis.
Polypeptide is divided into natural bioactive polypeptide and two kinds of artificial synthetic polypeptide according to its source.Wherein, natural bioactive polypeptide is
It directly extracts and obtains from organism, usually there is stronger activity, therefore, there is preferable effect to organism.However, day
Right biologically active polypeptide is due to extraction process complexity, so that its extraction cost is higher, simultaneously as living organism is complex,
Therefore, the purity of the polypeptide extracted is usually lower.Artificial synthetic polypeptide is to be closed using amino acid as raw material by chemical method
At polypeptide, however artificial synthesized polypeptide is without activity mostly, and therefore, it is necessary to can obtain by a large amount of screening verification
Active polypeptide sequence.Small-molecular peptides refer to that the polypeptide being made of 2~10 amino acid, molecular weight are usually less than
1000.Since molecular weight is smaller, small-molecular peptides are easier to be absorbed by living organism and bioavailability is higher.In recent years messenger
Work synthesized micromolecule peptide has been increasingly becoming the research hotspot of numerous scientific research scholars.
Plant except need the elements such as potassium, phosphorus or nitrogen as nutriment in addition to, it is also necessary to absorb minimal amount of iron, boron, arsenic, manganese,
Element necessary to the vital movements such as copper, cobalt molybdenum, these elements are referred to as microelement.When plant lacks microelement,
The resistivity of extraneous poor environment is reduced, growth and development performance is abnormal, it is therefore necessary to ensure that plant is during the growth process
Enough microelements can be absorbed into from environment.Influence plant in the factor of absorption of trace elements, in addition to external environment,
The content for regulating and controlling the growth regulator of plant own absorption ability is also of great significance.These growth regulators are usually by planting
Object itself synthesis, and the plant weaker for partially synthetic ability, if by applying the artificial conjunction that can be absorbed and used by plants
It also will be of great advantage for plant cultivation process at class growth regulator.Artificial synthesized small-molecular peptides, which have, is used as plant growth tune
The potentiality for saving agent, however, relevant report in the prior art is also relatively fewer.
Summary of the invention
It is easily absorbed and used by plants and to various plants the first technical problem to be solved by the present invention is: providing one kind
Mineral element absorbs the artificial synthesized small-molecular peptides with good facilitation.
Second technical problem to be solved by this invention is: providing a kind of growth tune containing artificial synthesized small-molecular peptides
Save agent.
Third technical problem to be solved by this invention is: providing a kind of application method of above-mentioned growth regulator.
In order to solve above-mentioned first technical problem, the solution of the present invention are as follows: a kind of artificial synthesized small-molecular peptides are described small
The amino acid sequence of molecular peptide is RPPFKLIPN (as shown in Seq ID No.1).
In order to solve above-mentioned second technical problem, the solution of the present invention are as follows: a kind of growth regulator, the growth regulating
Contain above-mentioned artificial synthesized small-molecular peptides in agent.
Further, the growth regulator is the growth regulator for promoting plant absorption mineral element.
Preferably, the mineral element is trace mineral element.
It is highly preferred that the mineral element includes at least one of Mg, Ca, Mn, Fe, Zn, Cu or B.
It is highly preferred that the mineral element includes Mg, Ca, Mn, Fe, Zn, Cu and B.
In order to solve above-mentioned third technical problem, the solution of the present invention are as follows: a kind of application side of above-mentioned growth regulator
Method, the application method is the following steps are included: be configured to the root that solution is applied to plant for the artificial synthesized small-molecular peptides
Or on blade.
Further, the concentration of artificial synthesized small-molecular peptides described in the solution is 0.05~0.5 μm of ol/L；It is preferred that
Ground, the concentration are 0.1 μm of ol/L.
Further, described to apply the Seedling Stage implementation operated be in plant.
Preferably, the plant is crucifer.
It is highly preferred that the plant is arabidopsis or rape.
The beneficial effects of the present invention are: the present invention program provides a kind of small-molecular peptides structure for containing only 9 amino acid,
The small-molecular peptides structure is simple, is easily absorbed and used by plants, and can not only be utilized by the root absorption of plant, can also be in quilt
To root transmitting signal so as to cause the variation of microelement in root after blade absorption；The polypeptide of the present invention program structure only needs micro-
Amount addition can play significant facilitation to the absorption process of a variety of mineral elements, meanwhile, the polypeptide is also to plant growth
With certain facilitation.
Detailed description of the invention
Fig. 1 is the first group and second group seedling (A) and its length measurement after culture 15 days in the embodiment of the present invention
Scheme (B)；
Fig. 2 is that blade applies and do not apply the plant for the peduncle-growing period for rapeseed that artificial synthesized small-molecular peptides are cultivated in the embodiment of the present invention
(A) and its aerial part weight measurement result figure (B)；
Fig. 3 is the Arabidopsis leaf (A) and root group that root is applied with artificial synthesized small-molecular peptides in the embodiment of the present invention
Knit the relative expression quantity histogram (B) of middle gene.
Specific embodiment
In order to describe the technical content, the structural feature, the achieved object and the effect of this invention in detail, below in conjunction with embodiment
And attached drawing is cooperated to be explained in detail.
The embodiment of the present invention are as follows: a kind of artificial synthesized small-molecular peptides, the amino acid sequence of the artificial synthesized small-molecular peptides
For RPPFKLIPN.Above-mentioned artificial synthesized small-molecular peptides are configured to solution and are applied to Arabidopsis thaliana Seedlings, due to verifying it to plant
The facilitation of growth and absorption of trace elements.
Entrust Wuhan Sino-American Biotechnology Company according to artificial synthesized small-molecular peptides described in above-mentioned sequent synthesis.
One, the culture and processing of Arabidopsis thaliana Seedlings:
It is prepared with 340mL tissue culture without artificial synthesized small-molecular peptides (first group) and containing artificial synthesized small point of 0.1 μm of ol/L
The solidified MS media of sub- peptide (second group) contains 40mL solid medium in each culture bottle.Arabidopsis seed with 70% wine
Essence sterilizing 5min, after sterile water wash 4 times, point is sowed on the MS solid medium (containing artificial synthesized small-molecular peptides) respectively.4℃
Dark vernalization is placed on culture (22 DEG C, 16 hours illumination/8 hour dark) under long-day conditions for 48 hours.
Two, influence of the artificial synthesized small-molecular peptides to plant growth:
Shown in arabidopsis young root such as Fig. 1 (A) after aforesaid operations culture 15 days, 11 plants first group and second is measured respectively
The length of the young root of group, shown in averaged result such as Fig. 1 (B), from figure 1 it appears that being added with artificial synthesized small point
The Arabidopsis thaliana Seedlings root long degree of sub- peptide is slightly longer than the Arabidopsis thaliana Seedlings for being not added with artificial synthesized small-molecular peptides culture medium culture.
It takes the Arabidopsis thaliana Seedlings of first group of culture to carry out potting culture under equal conditions, take plant height consistent and is initially located in pumping
Two plants of the Arabidopsis plant of a kind of sedge phase, wherein one plant to the artificial synthesized small-molecular peptides solution of 0.1 μm of ol/L of blade spraying, another plant of spray
Apply the water of equivalent.After continuing culture 5 days, shown in the Arabidopsis plant of acquisition such as Fig. 2 (A).As can be seen that spraying from Fig. 2 (A)
There is the Arabidopsis plant of artificial synthesized micromolecule peptide solution to be slightly above another strain.The weight of two plants of above-ground plant parts is weighed, is tied
Shown in fruit such as Fig. 2 (B), it can be seen from the figure that the weight for spraying artificial synthesized micromolecule peptide solution, which is greater than, does not spray group.
It can be seen from the results above that the artificial synthesized small-molecular peptides of the present invention program growth course entire for plant is equal
There is certain facilitation.
Three, influence of the artificial synthesized small-molecular peptides to absorption of trace elements:
1, root applies:
First group and second group 20 plants of progress trace element analysis of Arabidopsis thaliana Seedlings after culture 15 days are taken, respectively to root
And blade is analyzed.
PROCEDURE FOR SAMPLE PREPARATION specifically: take the fresh sample of arabidopsis respectively at 105 DEG C 20min finish, 80 DEG C of dry 30min with
On, until weight is constant.0.1g or so dry sample (root or leaf) is taken to be placed in HNO3/H2O2In (7:1.5, V/V), digested at 120 DEG C
10min, 139 DEG C of 20min, when temperature is lower than 50 DEG C, (the towering like a mountain peak Yao's instrument in Shanghai is scientific and technological for the sour instrument of catching up with for taking sample to be placed into 120 DEG C
Development Co., Ltd) in catch up with sour 30min, be settled to 100mL after cooling to room temperature, filter 10mL with 5 μm of filtering heads, take 2.5mL
It injects ICP-MS (Agilent 7500) and measures ion concentration.
ICP-MS test condition: Bablington atomizer with high salt；Quartzy binary channels scott spray chamber, quartz integration
Quarter bend, 1.5mm central passage.200 DEG C of room temperature of atomization；1.0/0.4mm (Ni) sampling cone；Flow rate of carrier gas 0.85L/min；High frequency
Transmission power 1450W；Mixed gas flow 0.28L/min；Sample promotes rate 0.1r/s；Plasma gas flow rate 15.0L/min；It adopts
Sample depth 7.0mm；Secondary air amount 1.0L/min；Sample lifting capacity 0.4mL/min；Helium gas flow 5mL/min.
To the content (μ g/g) of Mg, Ca, Mn, Fe, Zn, Cu, B and Mo that root samples measure, asks and calculate average results such as
Shown in the following table 1:
Arabidopsis root Mineral Elements assay result table when 1 root of table applies
As can be seen from the table, after the artificial synthesized small-molecular peptides processing of the present invention program, in addition to molybdenum, Arabidopsis thaliana Seedlings root
In the contents of the elements such as magnesium, calcium, manganese, iron, zinc, copper and boron significantly rise.Wherein, the variation of magnesium, calcium, manganese, iron and zinc reaches
To extremely significant horizontal (P < 0.01), Cu and B element variation are the level of signifiance (P < 0.05).Due to showing the people of the present invention program
Work synthesized micromolecule peptide can directly induce absorption and accumulation of the arabidopsis root to microelement.
To the content (μ g/g) of Mg, Ca, Mn, Fe, Zn, Cu, B and Mo that leaf sample measures, asks and calculate average results such as
Shown in the following table 2:
Blade Mineral Elements assay result table when 2 root of table applies
From Table 2, it can be seen that larger with result difference in root, artificial synthesized small-molecular peptides processing compared with the control, is intended
The content significant (P<0.05) for only having manganese, iron and zinc in southern mustard leaf rises, and Mg and Cu have downward trend (P>0.05), Ca, B instead
There is no significant change with Mo content.It can be seen that artificial synthesized small-molecular peptides mainly play a role in root, promote microelement
Absorption and accumulation in root, while possible inducing moiety ion is transported to aerial part, so as to cause the change of leaf ions content
Change.
2, blade applies:
It is molten that 20 plants of Arabidopsis thaliana Seedlings after taking first group of culture 15 days directly apply artificial synthesized small-molecular peptides on blade face
Liquid (0.1 μm of ol/L) carries out trace element analysis, measures micronutrient levels in seedling after then proceeding to culture 5 days.Sample preparation and
Determination step is consistent in the application of above root.Similarly, the microelement in root and blade is measured respectively.
To root measurement result averaged (μ g/g), as a result as shown in table 3 below:
Root Mineral Elements assay result table when 3 blade of table applies
After artificial synthesized small-molecular peptides solution processing arabidopsis leaf, Ye Zhong Fe and Zn content has significant (P < 0.05) to improve,
The variation of the constituent contents such as Mg, Ca, Mn, Cu, B and Mo is not obvious.It can be seen that artificial synthesized small-molecular peptides may can pass through
Blade is absorbed into plant.
To determination of trace element result averaged (μ g/g) in blade, as a result as shown in table 4 below:
Root Mineral Elements assay result table when 4 blade of table applies
As can be seen from the above table, manually after synthetic peptide processing Arabidopsis leaf, micronutrient levels and root in root
It is almost the same to apply result, the constituent contents such as Mg, Ca, Mn, Fe, Zn and Cu have to be promoted to some extent, wherein Mg, Ca, Fe and
Up to extremely significant horizontal (P < 0.01), Mn and Cu element variation is the level of signifiance (P < 0.05) for the variation of Zn.Arabidopsis after leaf processing
B and Mo element difference is unobvious in root.The result, which further demonstrates Arabidopsis leaf, can directly absorb the present invention program people
Work synthesized micromolecule peptide is to play physiological function.Also illustrate that the artificial synthesized small-molecular peptides of the present invention program may can also be with simultaneously
It transports to root downwards and plays a role from aerial part.
Four, influence of the artificial synthesized small-molecular peptides to the accumulation of non-essential element:
Part non-essential element content is measured in the Arabidopsis plant obtained to above-mentioned first group and second group of culture,
As a result (average value) is as shown in table 5 below:
5 root of table apply when arabidopsis root and blade in non-essential element assay result table
Measurement sample size in table is similarly 20 plants.
As can be seen from the above table, Arabidopsis thaliana Seedlings, aluminium in root are cultivated in the culture medium containing artificial synthesized small-molecular peptides
(Al), chromium (Cr), cobalt (Co) and lead (Pb) element have significant (P < 0.05) to improve, Cr and Co constituent content difference is not in leaf
Greatly, but Al and Pb are significantly increased (P < 0.05).
Five, the influence that artificial synthesized small-molecular peptides express absorption of trace elements transporter gene:
By studying the artificial synthesized small-molecular peptides of the present invention program to the regulating and controlling effect of absorption of trace elements, selected part generation
The mineral element absorption transporter gene of table simultaneously carries out network analysis to its expression.For Mg, Ca, Mn, Fe, Zn, Cu,
The absorption and transport body gene of Mo and B, respectively choose Gene A tMGT1, AtCAX4, AtNRAMP1, AtIRT1, AtMTP1,
AtCOPT1, AtMOT1 and AtNIP5；1 expression quantity is as research object.
Arabidopsis thaliana Seedlings (15 day age) is taken out from first group of culture medium, is placed in containing artificial synthesized small-molecular peptides (0.1 μ
Mol/L solidified MS media) continues sampling analysis gene expression after culture 6h.Take 0.1g or so Arabidopsis thaliana Seedlings tissue (leaf
Piece or root), total serum IgE is extracted using Trizol (Invitrogen) reagent, referring to M-MLV kit (Invitrogen) specification
CDNA is obtained using poly (dT) primer, reaction solution is configured referring to PCR kit for fluorescence quantitative specification, in CFX96
Response procedures: 95 DEG C of initial denaturation 30s are run in Real-time system (BIO-RAD), then run 40 circulations, including 95
DEG C denaturation 10s, 58 DEG C of annealing 30s and 72 DEG C of extension 10s, internal reference Actin.Such as 6 institute of table of primer involved in the present invention program
Show:
6 primer sequence table of table
Such as Fig. 3 (A) and 3 (B) are shown respectively for the relative expression quantity of gene in the Arabidopsis leaf and root tissue measured, from
In Fig. 3 (A) and 3 (B) as can be seen that after the processing of artificial synthesized small-molecular peptides, except molybdenum element absorption and transport Gene A tMOT1 is expressed
Other than significant change, AtMGT1, AtCAX4, AtNRAMP1, AtIRT1, AtMTP1, AtCOPT1 and AtNIP5 in root；1 equal bases
The expression of cause is significantly raised；After artificial synthesized small-molecular peptides processing, only AtIRT1 and AtMTP1 gene expression is aobvious in arabidopsis leaf
Write up-regulation.It can be seen that artificial synthesized small-molecular peptides processing arabidopsis root can induce absorption of trace elements transporter gene to express.
Similarly, it similarly can induce the expression of these genes in other plant, therefore, the artificial conjunction of the present invention program
Equally there is facilitation for the absorption of the mineral element of other plant at small-molecular peptides.
In conclusion root apply 0.1 μm of ol/L artificial synthesized small-molecular peptides can remarkably promote Mg, Ca in root,
The accumulation of the microelements such as Mn, Fe, Zn, Cu and B, blade apply artificial synthesized small-molecular peptides be remarkably improved Mg, Ca in root,
The content of the elements such as Mn, Fe, Zn and Cu, artificial synthesized small-molecular peptides influence micronutrient levels in arabidopsis leaf smaller；People
Work synthesized micromolecule peptide can significantly induce the absorption and transport body gene expression of the elements such as Mg, Ca, Mn, Fe, Zn, Cu and B in root
Therefore up-regulation illustrates that plant roots and Ye Jun are absorbable using the artificial synthesized micromolecule peptide, can be as growth regulating
Agent.The artificial synthesized micromolecule peptide, which only contains 9 amino acid residues, to cause micronutrient levels to change, and activity pole
It is low, it has a good application prospect.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright specification and accompanying drawing content is applied directly or indirectly in other relevant skills
Art field, is included within the scope of the present invention.
Sequence table
<110>Agricultural University Of Hunan
<120>a kind of artificial synthesized small-molecular peptides, growth regulator and its application method containing the artificial synthesized micromolecule peptide
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 9
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 1
Arg Pro Pro Phe Lys Leu Ile Pro Asn
1 5
Claims (10)
1. a kind of artificial synthesized small-molecular peptides, it is characterised in that: the amino acid sequence of the small-molecular peptides is RPPFKLIPN.
2. a kind of growth regulator, it is characterised in that: containing as described in claim 1 artificial synthesized in the growth regulator
Small-molecular peptides.
3. growth regulator according to claim 2, it is characterised in that: the growth regulator is for promoting plant to inhale
Receive the growth regulator of mineral element.
4. growth regulator according to claim 3, it is characterised in that: the mineral element is trace mineral element.
5. growth regulator according to claim 4, it is characterised in that: the mineral element include Mg, Ca, Mn, Fe,
At least one of Zn, Cu or B.
6. a kind of application method of such as described in any item growth regulators of claim 2-5, it is characterised in that: the application side
Method is applied on the root or blade of plant the following steps are included: the artificial synthesized small-molecular peptides are configured to solution.
7. application method according to claim 6, it is characterised in that: artificial synthesized small-molecular peptides described in the solution
Concentration is 0.05~0.5 μm of ol/L.
8. application method according to claim 7, it is characterised in that: the concentration is 0.1 μm of ol/L.
9. application method according to claim 6, it is characterised in that: described to apply the Seedling Stage implementation operated be in plant
's.
10. application method according to claim 6, it is characterised in that: the plant is crucifer, it is preferable that
The plant is arabidopsis or rape.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116444621A (en) * | 2023-05-16 | 2023-07-18 | 南京农业大学 | Small peptide for promoting plant root system development and application thereof |
Citations (2)
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| CN102172246A (en) * | 2010-12-29 | 2011-09-07 | 北京中农瑞利源高科技发展有限公司 | Plant growth regulator and application thereof in crops |
| CN108464303A (en) * | 2018-02-06 | 2018-08-31 | 浦江县欧立生物技术有限公司 | The preparation method of plant growth regulator |
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2019
- 2019-05-15 CN CN201910401957.XA patent/CN110229213B/en active Active
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| CN102172246A (en) * | 2010-12-29 | 2011-09-07 | 北京中农瑞利源高科技发展有限公司 | Plant growth regulator and application thereof in crops |
| CN108464303A (en) * | 2018-02-06 | 2018-08-31 | 浦江县欧立生物技术有限公司 | The preparation method of plant growth regulator |
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| 朱睿等: "几种植物生长调节剂对铁皮石斛生长发育的影响", 《时珍国医国药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116444621A (en) * | 2023-05-16 | 2023-07-18 | 南京农业大学 | Small peptide for promoting plant root system development and application thereof |
| CN116444621B (en) * | 2023-05-16 | 2024-03-29 | 南京农业大学 | Small peptide for promoting plant root system development and application thereof |
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