CN110229213B - Artificially synthesized small molecular peptide, growth regulator containing artificially synthesized small molecular peptide and application method of artificially synthesized small molecular peptide - Google Patents
Artificially synthesized small molecular peptide, growth regulator containing artificially synthesized small molecular peptide and application method of artificially synthesized small molecular peptide Download PDFInfo
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- CN110229213B CN110229213B CN201910401957.XA CN201910401957A CN110229213B CN 110229213 B CN110229213 B CN 110229213B CN 201910401957 A CN201910401957 A CN 201910401957A CN 110229213 B CN110229213 B CN 110229213B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
- A01H6/201—Brassica juncea
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
- A01H6/202—Brassica napus [canola]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
Abstract
The invention discloses an artificially synthesized small molecular peptide, a growth regulator containing the artificially synthesized small molecular peptide and an application method thereof, wherein the amino acid sequence of the small molecular peptide is shown as Seq ID No. 1. The growth regulator is used for promoting the plants to absorb mineral elements. The mineral elements include at least one of Mg, Ca, Mn, Fe, Zn, Cu, or B. The application method comprises the following steps: the artificially synthesized small molecule peptide is prepared into a solution and applied to the roots or leaves of plants. The micromolecular peptide has a simple structure, is easy to be absorbed and utilized by plants, can be absorbed and utilized by the roots of the plants, and can transmit signals to the roots after being absorbed by the leaves so as to cause the change of trace elements in the roots; the peptide can obviously promote the absorption process of various mineral elements by only adding trace amount of the peptide, and meanwhile, the polypeptide also has a certain promotion effect on plant growth.
Description
Technical Field
The invention relates to the technical field of biology, in particular to an artificially synthesized small molecular peptide, a growth regulator containing the artificially synthesized small molecular peptide and an application method of the artificially synthesized small molecular peptide.
Background
Polypeptides are compounds in which alpha-amino acids are linked together by peptide bonds and are intermediates in the hydrolysis of proteins. The polypeptides are divided into natural bioactive polypeptides and artificially synthesized polypeptides according to the source of the polypeptides. Among them, natural bioactive polypeptides are directly extracted from organisms, and generally have strong activity, and thus have good effect on organisms. However, the extraction process of natural bioactive polypeptide is complex, which results in high extraction cost, and the biological organism is complex, so the purity of the extracted polypeptide is usually low. The artificially synthesized polypeptide is a polypeptide which is synthesized by a chemical method by taking amino acid as a raw material, however, most of the artificially synthesized polypeptides have no activity, and therefore, a large amount of screening and verification methods are needed to obtain an active polypeptide sequence. The small molecule peptide refers to a polypeptide consisting of 2-10 amino acids, and the molecular weight of the small molecule peptide is usually less than 1000. Due to the small molecular weight, the small molecular peptide is easier to be absorbed by organisms and has higher bioavailability. In recent years, artificial synthesis of small molecule peptides is becoming a research hotspot of many researchers.
Plants require elements such as potassium, phosphorus, or nitrogen as nutrients, and also absorb very small amounts of elements necessary for life activities such as iron, boron, arsenic, manganese, copper, cobalt, molybdenum, and these elements are called trace elements. When the plant lacks trace elements, the resistance to the external adverse environment is reduced, and the growth and development performance is abnormal, so that it is necessary to ensure that the plant can absorb enough trace elements from the environment during the growth process. Among factors influencing the absorption of trace elements by plants, the content of a growth regulator for regulating and controlling the absorption capacity of the plants is also of great significance except for the external environment. These growth regulators are usually synthesized by the plant itself, but for plants which are partially less capable of synthesis, it would also be of great benefit to the plant cultivation process if a growth regulator of the synthetic type which can be taken up by the plant could be applied. Artificially synthesized small molecule peptides have the potential to be used as plant growth regulators, however, related reports in the prior art are relatively few.
Disclosure of Invention
The first technical problem to be solved by the invention is as follows: provides an artificially synthesized small molecular peptide which is easy to be absorbed and utilized by plants and has good promotion effect on the absorption of various plant mineral elements.
The second technical problem to be solved by the invention is: a growth regulator containing artificially synthesized small-molecule peptide is provided.
The third technical problem to be solved by the invention is: provides an application method of the growth regulator.
In order to solve the first technical problem, the invention has the following scheme: an artificially synthesized small molecule peptide, wherein the amino acid sequence of the small molecule peptide is RPPFKLIPN (shown as Seq ID No. 1).
In order to solve the second technical problem, the invention has the following scheme: a growth regulator contains the artificially synthesized small molecular peptide.
Further, the growth regulator is a growth regulator for promoting mineral element absorption by plants.
Preferably, the mineral element is a trace mineral element.
More preferably, the mineral elements include at least one of Mg, Ca, Mn, Fe, Zn, Cu or B.
More preferably, the mineral elements include Mg, Ca, Mn, Fe, Zn, Cu and B.
In order to solve the third technical problem, the invention has the following scheme: an application method of the growth regulator comprises the following steps: the artificially synthesized small molecule peptide is prepared into a solution and applied to the roots or leaves of plants.
Further, the concentration of the artificially synthesized small molecular peptide in the solution is 0.05-0.5 mu mol/L; preferably, the concentration is 0.1. mu. mol/L.
Further, the applying operation is performed at the seedling stage of the plant.
Preferably, the plant is a crucifer.
More preferably, the plant is arabidopsis thaliana or brassica napus.
The invention has the beneficial effects that: the scheme of the invention provides a small molecular peptide structure only containing 9 amino acids, the small molecular peptide structure is simple and easy to be absorbed and utilized by plants, and the small molecular peptide structure not only can be absorbed and utilized by the roots of the plants, but also can transmit signals to the roots after being absorbed by leaves so as to cause the change of trace elements in the roots; the polypeptide with the structure of the scheme of the invention can obviously promote the absorption process of various mineral elements by only adding trace amount, and meanwhile, the polypeptide also has a certain promotion effect on plant growth.
Drawings
FIG. 1 is a graph (A) showing the results of measurement of the length of seedlings (A) cultured for 15 days in the first and second groups in the example of the present invention;
FIG. 2 is a graph showing the results of measuring the weight of the bolting-stage plants (A) and their overground parts cultured with and without artificial synthetic small molecule peptides applied to the leaves in the embodiment of the present invention;
FIG. 3 is a histogram showing the relative expression levels of genes in the leaf (A) of Arabidopsis thaliana and the root tissue to which a synthetic small molecule peptide was applied in the root of the present invention.
Detailed Description
In order to explain technical contents, structural features, and objects and effects of the present invention in detail, the following detailed description is given with reference to the accompanying drawings in conjunction with the embodiments.
The embodiment of the invention is as follows: an artificially synthesized small molecule peptide, wherein the amino acid sequence of the artificially synthesized small molecule peptide is RPPFKLIPN. The solution prepared by the artificially synthesized small molecular peptide is applied to arabidopsis seedlings, and the effect of the solution on promoting plant growth and trace element absorption is verified.
Entrusts Wuhan Huamei bioengineering limited company to synthesize the artificially synthesized small molecule peptide according to the sequence.
Firstly, culturing and treating arabidopsis seedlings:
340mL of tissue culture medium is used for preparing solid MS culture medium without artificially synthesized small molecular peptide (the first group) and with artificially synthesized small molecular peptide (the second group) of 0.1 mu mol/L, and 40mL of solid culture medium is contained in each culture bottle. The arabidopsis seeds are sterilized by 70 percent alcohol for 5min, washed by sterile water for 4 times and respectively sowed on MS solid culture media (containing artificially synthesized small molecular peptides). After vernalization in the dark at 4 ℃ for 48 hours, the cells were cultured under long-day conditions (22 ℃, 16 hours light/8 hours dark).
Secondly, the influence of artificially synthesized small molecule peptides on plant growth:
the length of the young root of Arabidopsis thaliana cultured for 15 days by the above procedure is measured for 11 young roots of the first and second groups, respectively, as shown in FIG. 1(A), and the average result is shown in FIG. 1(B), and it can be seen from FIG. 1 that the root length of the young root of Arabidopsis thaliana added with the artificially synthesized small molecule peptide is slightly longer than that of the young root of Arabidopsis thaliana cultured in the medium without the artificially synthesized small molecule peptide.
Carrying out pot culture on a first group of cultured arabidopsis seedlings under the same condition, taking two arabidopsis plants which have the same plant height and begin to be in the bolting stage, spraying 0.1 mu mol/L artificially synthesized small molecular peptide solution to leaves of one of the two arabidopsis plants, and spraying equivalent water to the other arabidopsis plant. After further cultivation for 5 days, the Arabidopsis plants obtained were as shown in FIG. 2 (A). As can be seen from fig. 2(a), the arabidopsis plants sprayed with the synthetic small molecule peptide solution were slightly higher than the other plants. The aerial parts of the two plants were weighed, and the results are shown in FIG. 2(B), from which it can be seen that the weight of the artificially synthesized small molecule peptide solution sprayed was greater than that of the non-sprayed group.
From the results, the artificially synthesized small molecule peptide provided by the scheme of the invention has a certain promotion effect on the whole growth process of plants.
Thirdly, artificially synthesizing the influence of the small molecular peptide on the absorption of the trace elements:
1. root application:
and (3) taking 20 arabidopsis seedlings cultured for 15 days in the first group and the second group for analyzing trace elements, and analyzing roots and leaves respectively.
The sample preparation operation specifically comprises: removing enzyme of fresh Arabidopsis thaliana sample at 105 deg.C for 20min, and drying at 80 deg.C for more than 30min until the weight is unchanged. Taking about 0.1g of dry sample (root or leaf) and placing in HNO3/H2O2(7:1.5, V/V), digesting at 120 ℃ for 10min, cooling to 139 ℃ for 20min, when the temperature is lower than 50 ℃, taking a sample, placing the sample in an acid dispelling instrument (Shanghai Yao instruments science and technology development Co., Ltd.) at 120 ℃ for dispelling acid for 30min, cooling to room temperature, diluting to 100mL, filtering by using a 5-micrometer filter head for 10mL, taking 2.5mL, and injecting ICP-MS (Agilent 7500) for measuring the ion content.
ICP-MS test conditions: a Bablington high salt nebulizer; the quartz double-channel scott atomizing chamber, the quartz integrated rectangular tube and the 1.5mm central channel. The temperature of the atomizing chamber is 200 ℃; 1.0/0.4mm (Ni) sampling cone; the flow rate of the carrier gas is 0.85L/min; high frequency transmit power 1450W; the flow rate of the mixed gas is 0.28L/min; the sample lifting rate is 0.1 r/s; the plasma gas flow is 15.0L/min; the sampling depth is 7.0 mm; the auxiliary gas flow is 1.0L/min; the sample lifting amount is 0.4 mL/min; the flow rate of the helium gas was 5 mL/min.
The measured Mg, Ca, Mn, Fe, Zn, Cu, B and Mo contents (. mu.g/g) of the root samples were averaged as shown in Table 1 below:
TABLE 1 measurement results of mineral element content in Arabidopsis thaliana root when root is applied
As can be seen from the table, after the artificial synthesis of the small molecule peptide provided by the scheme of the invention is treated, the contents of elements such as magnesium, calcium, manganese, iron, zinc, copper and boron in the root of the seedling of Arabidopsis are obviously increased except molybdenum. Wherein, the change of magnesium, calcium, manganese, iron and zinc reaches a very significant level (P <0.01), and the change of Cu and B elements reaches a significant level (P < 0.05). As the result shows, the artificially synthesized small molecular peptide of the scheme of the invention can directly induce the arabidopsis root to absorb and accumulate trace elements.
The measured contents (. mu.g/g) of Mg, Ca, Mn, Fe, Zn, Cu, B and Mo in the vane samples were averaged as shown in Table 2 below:
TABLE 2 table of the results of the measurement of mineral element content in the blade when the root is applied
As can be seen from Table 2, compared with the results in roots, the artificial synthesized small molecule peptide treatment has the advantages that the contents of manganese, iron and zinc only increase remarkably (P <0.05), Mg and Cu decrease inversely (P >0.05) and the contents of Ca, B and Mo do not change obviously in Arabidopsis leaves compared with the control. Therefore, the artificially synthesized small molecule peptide mainly plays a role in roots, promotes the absorption and accumulation of trace elements in the roots, and simultaneously can induce part of ions to be transported to the overground part, thereby causing the ion content in leaves to change.
2. Blade application:
taking 20 arabidopsis seedlings cultured for 15 days in the first group, directly applying an artificially synthesized small molecular peptide solution (0.1 mu mol/L) on the leaf surface for analyzing trace elements, and then continuously culturing for 5 days to determine the content of the trace elements in the seedlings. The sample preparation and measurement steps were as in root application above. Similarly, the trace elements in the roots and leaves were measured, respectively.
The root measurements were averaged (μ g/g) and are shown in Table 3 below:
TABLE 3 table of measurement results of mineral element content in root at the time of blade application
After arabidopsis leaves are treated by artificially synthesizing a small molecular peptide solution, the contents of Fe and Zn in the leaves are obviously improved (P is less than 0.05), and the content changes of elements such as Mg, Ca, Mn, Cu, B, Mo and the like are not obvious. Therefore, the artificially synthesized small molecule peptide can be absorbed into the plant body through the leaf.
The results of the trace element measurements in the leaves were averaged (μ g/g) and are shown in Table 4 below:
TABLE 4 table of measurement results of mineral element content in root at the time of blade application
From the above table, it can be seen that after the arabidopsis thaliana leaves are treated by the synthetic peptide, the content of trace elements in the roots is basically consistent with the root application result, and the content of elements such as Mg, Ca, Mn, Fe, Zn and Cu is improved to different degrees, wherein the change of Mg, Ca, Fe and Zn reaches a very significant level (P <0.01), and the change of Mn and Cu elements reaches a significant level (P < 0.05). After leaf treatment, the difference between B and Mo elements in the root of Arabidopsis is not obvious. The result further proves that the arabidopsis thaliana leaves can directly absorb the artificially synthesized small molecular peptide in the scheme of the invention so as to play a physiological function. Meanwhile, the scheme of the invention also indicates that the artificially synthesized small molecule peptide can be transported from the overground part to the root to play a role.
Fourthly, the influence of artificially synthesized small molecule peptides on the accumulation of non-essential elements:
the contents of some of the unnecessary elements in the Arabidopsis plants obtained by the first and second groups of cultivation were measured, and the results (average values) are shown in Table 5 below:
TABLE 5 measurement results of contents of unnecessary elements in root and leaf of Arabidopsis thaliana at the time of root application
The measurement sample amounts in the tables are also 20 strains.
From the above table, it can be seen that when arabidopsis seedlings are cultured in a culture medium containing artificially synthesized small molecule peptides, the elements of aluminum (Al), chromium (Cr), cobalt (Co) and lead (Pb) in the roots are all significantly increased (P <0.05), the content difference of Cr and Co in the leaves is not large, but both Al and Pb are significantly increased (P < 0.05).
Fifthly, the influence of artificially synthesized small molecular peptides on the expression of the trace element absorption transport gene:
by researching the regulation and control effect of the synthetic small molecular peptide on the trace element absorption, part of the representative mineral element absorption transporter genes are selected and the expression condition of the mineral element absorption transporter genes is systematically analyzed. Aiming at the genes of absorption transporters of Mg, Ca, Mn, Fe, Zn, Cu, Mo and B, genes AtMGT1, AtCAX4, AtNRAMP1, AtIRT1, AtMTP1, AtCOPT1, AtMOT1 and AtNIP5 are respectively selected; 1 as the object of the study.
Arabidopsis seedlings (15 days old) were removed from the first group of media, placed in solid MS medium containing artificially synthesized small molecule peptides (0.1. mu. mol/L), and sampled for analysis of gene expression after further 6h of culture. About 0.1g of arabidopsis thaliana seedling tissue (leaf or root) was taken, total RNA was extracted using trizol (Invitrogen) reagent, cDNA was obtained using poly (dt) primer with reference to M-MLV kit (Invitrogen) instructions, reaction solution was prepared with reference to fluorescent quantitative PCR kit instructions, and reaction program was run in CFX96 Real-time system (BIO-RAD): pre-denaturation at 95 ℃ for 30s, then 40 cycles were run, including denaturation at 95 ℃ for 10s, annealing at 58 ℃ for 30s and extension at 72 ℃ for 10s, with the internal parameter Actin. The primers involved in the protocol of the invention are shown in table 6:
TABLE 6 primer sequence Listing
The measured relative expression amounts of the genes in the leaf and root tissues of arabidopsis thaliana are shown in fig. 3(a) and 3(B), respectively, and it can be seen from fig. 3(a) and 3(B) that after artificial synthesis of small molecule peptides, the expression of AtMGT1, AtCAX4, AtNRAMP1, AtIRT1, AtMTP1, AtCOPT1 and AtNIP5 in the root are not significantly changed except for the expression of the molybdenum element absorption transporter gene AtMOT 1; 1, etc. genes are obviously up-regulated; after artificial synthesis of small molecule peptide, only AtIRT1 and AtMTP1 genes in Arabidopsis leaves are significantly up-regulated. Therefore, the arabidopsis root treated by artificially synthesized small molecular peptide can induce the expression of trace element absorption transport genes.
Similarly, the expression of the genes can be induced in other plants, so that the artificially synthesized small molecule peptide provided by the scheme of the invention has the promotion effect on the absorption of mineral elements of other plants.
In conclusion, the application of 0.1 mu mol/L artificially synthesized small molecular peptide on the roots can remarkably promote the accumulation of trace elements such as Mg, Ca, Mn, Fe, Zn, Cu and B in the roots, the application of the artificially synthesized small molecular peptide on the leaves can remarkably improve the content of the elements such as Mg, Ca, Mn, Fe, Zn and Cu in the roots, and the artificially synthesized small molecular peptide has small influence on the content of the trace elements in the arabidopsis leaves; the artificially synthesized small molecular peptide can obviously induce the up-regulation of the gene expression of absorption transporters of Mg, Ca, Mn, Fe, Zn, Cu, B and other elements in roots, so that the roots and leaves of plants can absorb and utilize the artificially synthesized small molecular peptide, and the artificially synthesized small molecular peptide can be used as a growth regulator. The artificially synthesized small molecular peptide only contains 9 amino acid residues to cause the content change of trace elements, has extremely low action concentration and has good application prospect.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Sequence listing
<110> Hunan agriculture university
<120> artificially synthesized small molecule peptide, growth regulator containing the artificially synthesized small molecule peptide and application method thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 9
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 1
Arg Pro Pro Phe Lys Leu Ile Pro Asn
1 5
Claims (8)
1. An artificially synthesized small molecule peptide, which is characterized in that: the amino acid sequence of the small molecule peptide is RPPFKLIPN.
2. A growth regulator characterized by: the growth regulator comprises the artificially synthesized small-molecule peptide as claimed in claim 1.
3. A method of using the growth regulator of claim 2, wherein: the application method comprises the following steps: the artificially synthesized small molecule peptide is prepared into a solution and applied to the roots or leaves of plants.
4. The method of application according to claim 3, characterized in that: the concentration of the artificially synthesized small molecular peptide in the solution is 0.05-0.5 mu mol/L.
5. The method of application according to claim 4, characterized in that: the concentration was 0.1. mu. mol/L.
6. The method of application according to claim 3, characterized in that: the application is carried out at the seedling stage of the plant.
7. The method of application according to claim 3, characterized in that: the plant is a cruciferous plant.
8. The method of application according to claim 7, characterized in that: the plant is Arabidopsis thaliana or rape.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172246A (en) * | 2010-12-29 | 2011-09-07 | 北京中农瑞利源高科技发展有限公司 | Plant growth regulator and application thereof in crops |
| CN108464303A (en) * | 2018-02-06 | 2018-08-31 | 浦江县欧立生物技术有限公司 | The preparation method of plant growth regulator |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172246A (en) * | 2010-12-29 | 2011-09-07 | 北京中农瑞利源高科技发展有限公司 | Plant growth regulator and application thereof in crops |
| CN108464303A (en) * | 2018-02-06 | 2018-08-31 | 浦江县欧立生物技术有限公司 | The preparation method of plant growth regulator |
Non-Patent Citations (2)
| Title |
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| A peptide hormone required for Casparian strip diffusion-barrier formation in Arabidopsis roots;Takuya Nakayama等;《Science》;20171231;第284-286页 * |
| 几种植物生长调节剂对铁皮石斛生长发育的影响;朱睿等;《时珍国医国药》;20171231;第28卷(第11期);第2737-2740页 * |
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