CN110208551A - Chronic kidney disease marker supar easy detection device - Google Patents
Chronic kidney disease marker supar easy detection device Download PDFInfo
- Publication number
- CN110208551A CN110208551A CN201910619833.9A CN201910619833A CN110208551A CN 110208551 A CN110208551 A CN 110208551A CN 201910619833 A CN201910619833 A CN 201910619833A CN 110208551 A CN110208551 A CN 110208551A
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- China
- Prior art keywords
- detection
- pad
- box body
- kidney disease
- chronic kidney
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
Abstract
The invention discloses chronic kidney disease marker supar easy detection devices, including detecting box body, test trough, imbibition pad, imbibition ring, detect composite pad, sample pad, NC film, first conductive terminal, second conductive terminal, warning light, pass through the mating reaction of the anti-human supar monoclonal antibody 1- nanometer fluorescent microspheres compound of mouse and rabbit-anti chicken IgY- nanometer fluorescent microspheres compound in detection composite pad first, it can be realized detection of the rapid and convenient to chronic kidney disease marker supar, specificity and high sensitivity, also clinical operation is facilitated, greatly reduce use cost, practicability is expanded, secondly by the design of dropping liquid prompting mechanism, it can also ensure that effective contact of detection liquid, improve the accuracy of detection, effectively avoid the generation of invalid detection, it is finally reached very well Clinical application purpose.
Description
Technical field
The present invention relates to Medical Equipment field more particularly to chronic kidney disease marker supar easy detection devices.
Background technique
Kidney trouble (KD) is chronic Renal Structure and dysfunction as caused by a variety of causes, kidney trouble it is many kinds of,
More typical has acute glomerulonephritis and the related pyelonephritis of bacterium infection caused by immune injure, in addition diabetes, hypertension
And the patients such as systemic lupus erythematosus also Chang Bingfa renal lesions.Nearly 10 Yu Nianlai, at the international level in chronic kidney disease
(CKD) research becomes the hot spot being widely noticed.Human urokinase type Plasminogen activation receptor (uPAR) is a kind of height glycosyl
The protein of change is a multifunctional receptor in fibrinolytic system, has 3 homeodomains such as D1, D2 and D3, passes through two sulphur
Key connection, expresses in the cells such as neutrophil leucocyte, phagocyte, active t cell, endothelial cell and cancer cell, can be used as urine
The receptor of kinases and some other transmembrane protein, by the activation, the signal transmitting, cell that mediate plasminogen with its ligand binding
Stick and the different physiological roles such as cell transfer.Discharge into that become in Peripheral Circulation can when uPAR falls off from cell surface
Dissolubility uPAR Research (supar), supar are the specific receptors of uPAR, are widely present in vivo
On a variety of haemocytes, vascular endothelial cell, smooth muscle cell and tumor cell membrane.Researches show that detection supar haemoconcentration energy
Before disease initially causes tissue damage in 5 years reliable prediction kidney trouble onset risk, this will likely improve kidney trouble
Early detection, prevention and monitoring, therefore supar can be used as chronic kidney disease early diagnosis marker.It builds both at home and abroad at present
It is vertical quickly and effectively to the detection method of supar, mainly using enzyme-linked immunization (ELISA), chemoluminescence method, gold mark chromatography
The detection of the methods of method, biochip, but it is these method higher costs, complicated for operation, in addition, existing some detection devices, nothing
Method prompts detecting state well, then leverages the accuracy of detection, in consideration of it, it is necessary to design chronic kidney disease mark
Will object supar easy detection device solves the above problems.
Summary of the invention
Technical problem to be solved by the present invention lies in: chronic kidney disease marker supar easy detection device is provided, is come
It solves the problem of at present because of traditional detection method higher cost, complicated for operation and influence detection accuracy.
In order to solve the above technical problems, the technical scheme is that chronic kidney disease marker supar easy detection fills
It sets, including detection box body, test trough, imbibition pad, imbibition ring, detection composite pad, sample pad, NC film, the first conductive terminal, second
Conductive terminal, warning light, the detection box body be it is cylindric, the test groove is and described in detection tray interior
Test trough is circular groove, and the imbibition pad is fixedly arranged on test trough inner bottom, and the imbibition ring is fixedly arranged on imbibition pad top
Portion's surrounding, the detection composite pad are fixedly arranged on imbibition ring inner wall, and the sample pad is fixedly arranged on detection composite pad inner wall, described
NC film be fixedly arranged at the top of detection composite pad and sample pad, it is left that first conductive terminal is fixedly arranged on detection tray interior lower end
Side, second conductive terminal is fixedly arranged on the right side of detection tray interior lower end, and first conductive terminal and second is led
Electric terminal is contacted with the fitting of imbibition pad respectively, and the warning light is fixedly arranged on detection tray interior bottom end, and the warning light
Anode is connect with the first conductive terminal using conducting wire.
Further, box cover is also arranged at the top of the detection box body, the box cover and detection box body use inside and outside spiral shell
Line rotation connection.
Further, mark slot is fixedly provided at the top of the box cover, the mark slot and box cover are integrally formed.
Further, the detection tray interior upper end is fixedly provided with refluence cover, and the refluence cover is adopted with detection box body
It is connected with adhesive, and the refluence cover is transparent PVC material.
Further, middle-end is additionally provided with dripping hole inside the refluence cover, and the dripping hole is circular through hole.
Further, the detection box body lower end side is additionally provided with battery, and the battery and detection box body use
It snaps connection, and the accumulator anode and cathode is connect with the second conductive terminal and warning light using conducting wire respectively.
Further, the detection box body bottom one circle is fixedly provided with transparent ring, and the transparent ring is adopted with detection box body
It is connected with adhesive, and the transparent ring is transparent PVC material.
Further, the detection composite pad by the anti-human supar monoclonal antibody 1- nanometer fluorescent microspheres compound of mouse and
Rabbit-anti chicken IgY- nanometer fluorescent microspheres compound composition.
Compared with prior art, the chronic kidney disease marker supar easy detection device passes through detection composite pad first
In the anti-human supar monoclonal antibody 1- nanometer fluorescent microspheres compound of mouse and rabbit-anti chicken IgY- nanometer fluorescent microspheres compound
Mating reaction can be realized detection of the rapid and convenient to chronic kidney disease marker supar, specificity and high sensitivity, also side
Clinical operation, greatly reduce use cost, expanded practicability, secondly by the design of dropping liquid prompting mechanism,
The effective contact that can also ensure that detection liquid, improves the accuracy of detection, effectively avoids the generation of invalid detection, finally
Reach good clinical application purpose.
Detailed description of the invention
Fig. 1 is the main view of chronic kidney disease marker supar easy detection device；
Fig. 2 is the top view of chronic kidney disease marker supar easy detection device；
Fig. 3 is the perspective view of chronic kidney disease marker supar easy detection device；
Fig. 4 is the A of chronic kidney disease marker supar easy detection device to sectional block diagram；
Fig. 5 is the disassembly status perspective view of chronic kidney disease marker supar easy detection device；
Fig. 6 is the circuit diagram of chronic kidney disease marker supar easy detection device.
It is conductive to detect box body 1, test trough 2, imbibition pad 3, imbibition ring 4, detection composite pad 5, sample pad 6, NC film 7, first
Terminal 8, warning light 10, box cover 101, mark slot 102, flows backwards cover 103, dripping hole 104, battery at second conductive terminal 9
105, transparent ring 106.
The following detailed description will be further explained with reference to the above drawings.
Specific embodiment
Hereinafter, a variety of specific details are elaborated, in order to provide to the saturating of the concept for constituting described embodiment basis
Thorough understanding, however, it will be apparent to those skilled in the art that described embodiment can be in these no specific details
In the practice of getting off of some or all situations do not specifically describe well-known processing step in other cases.
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4, Fig. 5, Fig. 6, including detection box body 1, test trough 2, imbibition pad 3, imbibition ring 4,
Detect composite pad 5, sample pad 6, NC film 7, the first conductive terminal 8, the second conductive terminal 9, warning light 10, the detection box body
1 is that cylindric, described test trough 2 is located inside detection box body 1, and the test trough 2 is circular groove, the imbibition
Pad 3 is fixedly arranged on 2 inner bottom of test trough, and the imbibition ring 4 is fixedly arranged on 3 top surrounding of imbibition pad, the detection composite pad 5
It is fixedly arranged on 4 inner wall of imbibition ring, the sample pad 6 is fixedly arranged on detection 5 inner wall of composite pad, and it is multiple that the NC film 7 is fixedly arranged on detection
6 top of pad 5 and sample pad is closed, first conductive terminal 7 is fixedly arranged on the left of detection 1 interior lower end of box body, and described second
Conductive terminal 9 is fixedly arranged on the right side of detection 1 interior lower end of box body, and first conductive terminal 8 and the second conductive terminal 9 difference
It is contacted with the fitting of imbibition pad 3, the warning light 10 is fixedly arranged on detection 1 inner bottom of box body, and 10 anode of warning light
It is connect with the first conductive terminal 8 using conducting wire；
When detection；After detecting liquid contact NC film 7, because around imbibition ring 4 acts on, the absorption to detection liquid can be improved,
It is easy to implement detection liquid and sample pad 6 is contacted by detection composite pad 5, i.e., it is glimmering by 1- nanometers of mouse anti-human supar monoclonal antibody
The mating reaction of light microsphere compound and rabbit-anti chicken IgY- nanometer fluorescent microspheres compound, can reach quick testing goal；
Meanwhile because of the effect of bottom imbibition pad 3, it then can be realized another part detection liquid and permeate downwards, when imbibition pad 3
After bottom percolation is complete, i.e., by the electrical conduction effect of detection liquid, it can be realized the first conductive terminal 8 of left and right and the second conducting end
Son 9 contacts with each other, i.e., battery 105 and warning light 10 generate energized state, final to realize that warning light 10 lights, and is convenient for medical care
Personnel are visual, so as to which ensuring to detect liquid has penetrated detection composite pad 5, finally improve the accuracy of testing result；
1 top of detection box body is also arranged with box cover 101, and the box cover 101 and detection box body 1 use inside and outside spiral shell
Line rotation connection, therefore box cover 101 can not only closed detection box body 1, be also convenient for Unscrew, facilitate clinical manipulation；
Be fixedly provided at the top of the box cover 101 mark slot 102, the mark slot 102 and the one of box cover 101 at
Type, therefore identify and be inserted into respective patient information inside slot 102, convenient for subsequent tracking or record etc.；
1 inner upper end of detection box body is fixedly provided with refluence cover 103, and the refluence cover 103 is adopted with detection box body 1
It is connected with adhesive, and the refluence cover 103 is transparent PVC material, therefore flow backwards cover 103 to reach to detection liquid
Downward guidance, while because flow backwards cover 103 be transparent material, be also convenient for medical staff through flow backwards cover 103 pairs lower section sample pads 6
It is observed；
The 103 inside middle-end of refluence cover is additionally provided with dripping hole 104, and the dripping hole 104 is circular through hole, diameter
For 3mm, therefore dripping hole 104 drips downwards convenient for detection liquid；
The 1 lower end side of detection box body is additionally provided with battery 105, and the battery 105 and detection box body 1 use
It snaps connection, and 105 positive and negative anodes of battery are connect with the second conductive terminal 9 and warning light 10 using conducting wire respectively, because
This battery 105 is to carry out energy supply to warning light 10；
1 bottom one of the detection box body circle is fixedly provided with transparent ring 106, and the transparent ring 106 is adopted with detection box body 1
It is connected with adhesive, and the transparent ring 106 is transparent PVC material, is highly 5mm, therefore the effect of transparent ring 106 can
Convenient for penetrating outside the light of warning light 10, medical staff is facilitated to check；
The detection composite pad 5 is by the anti-human supar monoclonal antibody 1- nanometer fluorescent microspheres compound of mouse and rabbit-anti chicken
IgY- nanometer fluorescent microspheres compound composition；
It to sum up, can not only by imbibition pad 3, imbibition ring 4, the fiting effect for detecting composite pad 5, sample pad 6 and NC film 7
It is enough to reach quick testing goal convenient for detection liquid contact transmitting, effectively avoid the generation of invalid detection, this fit on first
The effect of conductive terminal 8, the second conductive terminal 9 and warning light 10, also can be realized liquid conduction, and it is logical to reach triggering warning light 10
Electricity lights purpose, furthermore flows backwards cover 103 and dripping hole 104 also facilitates operation, is convenient for subsequent observation.
Claims (8)
1. chronic kidney disease marker supar easy detection device, it is characterised in that including detection box body, test trough, imbibition pad,
Imbibition ring, detection composite pad, sample pad, NC film, the first conductive terminal, the second conductive terminal, warning light, the detection box body
To be cylindric, for the test groove in detection tray interior, the imbibition pad is fixedly arranged on test trough inner bottom, described
Imbibition ring is fixedly arranged on surrounding at the top of imbibition pad, and the detection composite pad is fixedly arranged on imbibition ring inner wall, and the sample pad is fixed
In detection composite pad inner wall, the NC film is fixedly arranged at the top of detection composite pad and sample pad, and first conductive terminal is solid
On the left of detection tray interior lower end, second conductive terminal is fixedly arranged on the right side of detection tray interior lower end, and described
The first conductive terminal and the second conductive terminal respectively with imbibition pad fitting contact, the warning light be fixedly arranged on detection box body in
Portion bottom end, and the warning light anode is connect with the first conductive terminal using conducting wire.
2. chronic kidney disease marker supar easy detection device as described in claim 1, it is characterised in that the detection
Box cover, the box cover and detection box body is also arranged at the top of box body to connect using internal and external threads rotation.
3. chronic kidney disease marker supar easy detection device as claimed in claim 2, it is characterised in that the box cover
Top is fixedly provided with mark slot, and the mark slot and box cover are integrally formed.
4. chronic kidney disease marker supar easy detection device as described in claim 1, it is characterised in that the detection
Tray interior upper end is fixedly provided with refluence cover, and the refluence cover is connect with detection box body using adhesive.
5. chronic kidney disease marker supar easy detection device as claimed in claim 4, it is characterised in that the refluence
It covers internal middle-end and is additionally provided with dripping hole, the dripping hole is circular through hole.
6. chronic kidney disease marker supar easy detection device as described in claim 1, it is characterised in that the detection
Box body lower end side is additionally provided with battery, and the battery is used with detection box body and snapped connection, and the accumulator positive
Cathode is connect with the second conductive terminal and warning light using conducting wire respectively.
7. chronic kidney disease marker supar easy detection device as described in claim 1, it is characterised in that the detection
The circle of box body bottom one is fixedly provided with transparent ring, and the transparent ring is connect with detection box body using adhesive.
8. chronic kidney disease marker supar easy detection device as described in claim 1, it is characterised in that the detection
Composite pad is compound by the anti-human supar monoclonal antibody 1- nanometer fluorescent microspheres compound of mouse and rabbit-anti chicken IgY- nanometer fluorescent microspheres
Object composition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910619833.9A CN110208551B (en) | 2019-07-10 | 2019-07-10 | Convenient detection device for chronic kidney disease marker supar |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910619833.9A CN110208551B (en) | 2019-07-10 | 2019-07-10 | Convenient detection device for chronic kidney disease marker supar |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110208551A true CN110208551A (en) | 2019-09-06 |
| CN110208551B CN110208551B (en) | 2023-01-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910619833.9A Active CN110208551B (en) | 2019-07-10 | 2019-07-10 | Convenient detection device for chronic kidney disease marker supar |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000020862A1 (en) * | 1998-10-02 | 2000-04-13 | Abp Diagnostics Ltd. | Process and apparatus for the in vitro detection of multiple analytes |
| US20070172963A1 (en) * | 2003-06-03 | 2007-07-26 | Krauth Gary H | Native analyte as a reference in lateral flow assays |
| US20100322823A1 (en) * | 2008-06-04 | 2010-12-23 | Surapaneni Krishna P | Rapid Detection of Post-Vaccination Antibody Response |
| CN202442747U (en) * | 2012-02-15 | 2012-09-19 | 赵舜培 | Water detector |
| CN108303550A (en) * | 2018-02-09 | 2018-07-20 | 广东优尼德生物科技有限公司 | A kind of the spot gold diafiltration kit and its quantitative detecting method of detection adiponectin |
| CN208568802U (en) * | 2018-07-02 | 2019-03-01 | 上海凯璟生物科技有限公司 | A kind of chronic kidney disease marker suPAR rapid detection card |
-
2019
- 2019-07-10 CN CN201910619833.9A patent/CN110208551B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000020862A1 (en) * | 1998-10-02 | 2000-04-13 | Abp Diagnostics Ltd. | Process and apparatus for the in vitro detection of multiple analytes |
| US20070172963A1 (en) * | 2003-06-03 | 2007-07-26 | Krauth Gary H | Native analyte as a reference in lateral flow assays |
| US20100322823A1 (en) * | 2008-06-04 | 2010-12-23 | Surapaneni Krishna P | Rapid Detection of Post-Vaccination Antibody Response |
| CN202442747U (en) * | 2012-02-15 | 2012-09-19 | 赵舜培 | Water detector |
| CN108303550A (en) * | 2018-02-09 | 2018-07-20 | 广东优尼德生物科技有限公司 | A kind of the spot gold diafiltration kit and its quantitative detecting method of detection adiponectin |
| CN208568802U (en) * | 2018-07-02 | 2019-03-01 | 上海凯璟生物科技有限公司 | A kind of chronic kidney disease marker suPAR rapid detection card |
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| CN110208551B (en) | 2023-01-03 |
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