CN110208551B - Convenient detection device for chronic kidney disease marker supar - Google Patents
Convenient detection device for chronic kidney disease marker supar Download PDFInfo
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- CN110208551B CN110208551B CN201910619833.9A CN201910619833A CN110208551B CN 110208551 B CN110208551 B CN 110208551B CN 201910619833 A CN201910619833 A CN 201910619833A CN 110208551 B CN110208551 B CN 110208551B
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
The invention discloses a convenient detection device for chronic kidney disease marker subpar, which comprises a detection box body, a test groove, a liquid absorption pad, a liquid absorption ring, a detection composite pad, a sample pad, an NC membrane, a first conductive terminal, a second conductive terminal and a prompting lamp.
Description
Technical Field
The invention relates to the field of medical detection equipment, in particular to a convenient detection device for chronic kidney disease marker supar.
Background
Renal disease (KD) is a chronic structural and functional disorder of the kidney caused by various reasons, and the renal disease is of various types, more common glomerulonephritis caused by immune injury and pyelonephritis related to bacterial infection, and patients with diabetes, hypertension, systemic lupus erythematosus and the like also have complicated nephropathy. For over 10 years, the study of Chronic Kidney Disease (CKD) has become a focus of great interest internationally. The human urokinase-type plasminogen activating receptor (uPAR) is a highly glycosylated protein, is a multifunctional receptor in a fibrinolytic system, has 3 homologous domains such as D1, D2 and D3, is connected by disulfide bonds, is expressed in cells such as neutrophils, phagocytes, active T cells, endothelial cells, cancer cells and the like, can be used as a receptor of urokinase and other transmembrane proteins, and mediates various physiological functions such as plasminogen activation, signal transmission, cell adhesion, cell transfer and the like by combining with a ligand thereof. When the uPAR is shed from the cell surface and released into peripheral blood circulation, the uPAR becomes a soluble urokinase-type plasminogen activator receptor (supar), and the supar is a specific receptor of the uPAR and widely exists on various blood cells, vascular endothelial cells, smooth muscle cells and tumor cell membranes in vivo. Studies have shown that measuring the blood concentration of supar can reliably predict the risk of onset of kidney disease within 5 years before the disease begins to cause tissue damage, which will likely improve early detection, prevention and monitoring of kidney disease, and thus supar can be used as an early diagnostic marker for chronic kidney disease. At present, the fast and effective detection method for the subpar established at home and abroad mainly adopts methods such as an enzyme-linked immunosorbent assay (ELISA), a chemiluminescence method, a gold-labeled chromatography method, a biochip and the like for detection, but the methods have high cost and complex operation, and in addition, some existing detection equipment cannot well prompt the detection state, so that the detection accuracy is greatly influenced.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the convenient detection device for the chronic kidney disease marker supar is provided, and the problems that the traditional detection method is high in cost and complex in operation and detection accuracy is affected at present are solved.
In order to solve the technical problems, the technical scheme of the invention is as follows: the convenient detection device for chronic kidney disease marker supar comprises a detection box body, a test slot, a liquid absorption pad, a liquid absorption ring, a detection composite pad, a sample pad, an NC (numerical control) membrane, a first conductive terminal, a second conductive terminal and a prompting lamp, wherein the detection box body is cylindrical, the test slot is positioned inside the detection box body, the test slot is a circular groove, the liquid absorption pad is fixedly arranged at the bottom inside the test slot, the liquid absorption ring is fixedly arranged around the top of the liquid absorption pad, the detection composite pad is fixedly arranged on the inner wall of the liquid absorption ring, the sample pad is fixedly arranged on the inner wall of the detection composite pad, the NC membrane is fixedly arranged at the top of the detection composite pad and the sample pad, the first conductive terminal is fixedly arranged on the left side of the lower end inside the detection box body, the second conductive terminal is fixedly arranged on the right side of the lower end inside the detection box body, the first conductive terminal and the second conductive terminal are respectively in contact with the liquid absorption pad in a laminating manner, the prompting lamp is fixedly arranged on the bottom inside the detection box body, and the positive pole of the prompting lamp is connected with the first conductive terminal through a wire.
Further, the top of the detection box body is also sleeved with a box cover, and the box cover and the detection box body are in internal and external thread rotary connection.
Furthermore, the top of the box cover is fixedly provided with an identification slot, and the identification slot and the box cover are integrally formed.
Furthermore, the inside upper end of detection box body still set firmly the refluence cover, the refluence cover adopt the adhesive to be connected with the detection box body, just the refluence cover be transparent PVC material.
Furthermore, the inner middle end of the backflow hood is also provided with a liquid dropping hole, and the liquid dropping hole is a circular through hole.
Furthermore, detection box body lower extreme one side still be equipped with the battery, battery and detection box body adopt the buckle to be connected, just battery positive negative pole adopt the wire to be connected with second conductive terminal and warning light respectively.
Further, the round of detection box body bottom still set firmly transparent ring, transparent ring and detection box body adopt the adhesive to be connected, just transparent ring be transparent PVC material.
Furthermore, the detection composite pad is composed of a mouse anti-human supar monoclonal antibody 1-nano fluorescent microsphere compound and a rabbit anti-chicken IgY-nano fluorescent microsphere compound.
Compared with the prior art, the convenient detection device for the chronic kidney disease marker supar can realize quick and convenient detection of the chronic kidney disease marker supar through the cooperation of the mouse anti-human supar monoclonal antibody 1-nano fluorescent microsphere compound and the rabbit anti-chicken IgY-nano fluorescent microsphere compound in the detection composite pad, is high in specificity and sensitivity, brings convenience to clinical operation, greatly reduces the use cost and expands the practicability, can ensure effective contact of detection liquid through the design of a liquid dropping prompting mechanism, improves the detection accuracy, effectively avoids invalid detection, and finally achieves the good clinical popularization and application purposes.
Drawings
FIG. 1 is a front view of a convenient detection device for chronic kidney disease marker supar;
FIG. 2 is a top view of a convenient detection device for chronic kidney disease marker supra;
FIG. 3 is a perspective view of a convenient detection device for chronic kidney disease marker supar;
FIG. 4 is a perspective view of a chronic kidney disease marker supar convenient detection device in a cross-section A;
fig. 5 is a perspective view of the chronic kidney disease marker supar convenient detection device in a disassembled state;
fig. 6 is a circuit diagram of a convenient detection device for chronic kidney disease marker supar.
The detection device comprises a detection box body 1, a test slot 2, a liquid absorption pad 3, a liquid absorption ring 4, a detection composite pad 5, a sample pad 6, an NC membrane 7, a first conductive terminal 8, a second conductive terminal 9, a prompting lamp 10, a box cover 101, an identification slot 102, a backflow cover 103, a liquid dropping hole 104, a storage battery 105 and a transparent ring 106.
The following detailed description will be further described in conjunction with the above-identified drawings.
Detailed Description
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the concepts underlying the described embodiments, however, it will be apparent to one skilled in the art that the described embodiments may be practiced without some or all of these specific details, and in other cases well-known process steps have not been described in detail.
As shown in fig. 1, 2, 3, 4, 5, and 6, the detection kit comprises a detection kit body 1, a test slot 2, a liquid absorption pad 3, a liquid absorption ring 4, a detection composite pad 5, a sample pad 6, an NC film 7, a first conductive terminal 8, a second conductive terminal 9, and a notification lamp 10, wherein the detection kit body 1 is cylindrical, the test slot 2 is located inside the detection kit body 1, the test slot 2 is a circular groove, the liquid absorption pad 3 is fixedly arranged at the bottom end inside the test slot 2, the liquid absorption ring 4 is fixedly arranged around the top of the liquid absorption pad 3, the detection composite pad 5 is fixedly arranged on the inner wall of the liquid absorption ring 4, the sample pad 6 is fixedly arranged on the inner wall of the detection composite pad 5, the NC film 7 is fixedly arranged on the top of the detection composite pad 5 and the sample pad 6, the first conductive terminal 7 is fixedly arranged on the left side of the lower end inside the detection kit body 1, the second conductive terminal 9 is fixedly arranged on the right side of the lower end inside the detection kit body 1, the first conductive terminal 8 and the second conductive terminal 9 are respectively contacted with the liquid absorption pad 3, the notification lamp 10 is fixedly arranged on the bottom end of the detection kit body, and the detection kit body and the notification lamp is connected with the positive electrode of the first conductive terminal by a wire;
during detection; when the detection liquid contacts the NC membrane 7, the absorption of the detection liquid can be improved due to the action of the peripheral liquid absorption ring 4, so that the detection liquid can conveniently contact the sample pad 6 through the detection composite pad 5, namely, the rapid detection can be achieved through the cooperation of the mouse anti-human supar monoclonal antibody 1-nano fluorescent microsphere compound and the rabbit anti-chicken IgY-nano fluorescent microsphere compound;
meanwhile, due to the action of the bottom liquid absorption pad 3, the other part of detection liquid can permeate downwards, when the bottom of the liquid absorption pad 3 completely permeates, namely through the electric conduction action of the detection liquid, the mutual contact of the left and right first conductive terminals 8 and the second conductive terminals 9 can be realized, namely, the storage battery 105 and the prompting lamp 10 are in an electrified state, and finally the prompting lamp 10 is lightened, so that the visual observation of medical staff is facilitated, the detection liquid can be ensured to permeate the detection composite pad 5, and the accuracy of a detection result is finally improved;
the top of the detection box body 1 is also sleeved with a box cover 101, and the box cover 101 is rotationally connected with the detection box body 1 through internal and external threads, so that the box cover 101 not only can seal the detection box body 1, but also is convenient to rotate and open, and is convenient for clinical operation;
the top of the box cover 101 is fixedly provided with an identification slot 102, and the identification slot 102 and the box cover 101 are integrally formed, so that corresponding patient information can be inserted into the identification slot 102, and subsequent tracking or recording is facilitated;
the upper end in the detection box body 1 is fixedly provided with the backflow cover 103, the backflow cover 103 is connected with the detection box body 1 through an adhesive, and the backflow cover 103 is made of transparent PVC (polyvinyl chloride), so that the backflow cover 103 can guide detection liquid downwards, and meanwhile, medical workers can observe a sample pad 6 below through the backflow cover 103 due to the fact that the backflow cover 103 is made of transparent materials;
the middle end in the backflow cover 103 is also provided with a liquid dropping hole 104, the liquid dropping hole 104 is a circular through hole, the diameter is 3mm, and therefore the liquid dropping hole 104 is convenient for detecting liquid to drop downwards;
a storage battery 105 is further arranged on one side of the lower end of the detection box body 1, the storage battery 105 is connected with the detection box body 1 through a buckle, and the positive and negative electrodes of the storage battery 105 are respectively connected with the second conductive terminals 9 and the prompting lamps 10 through wires, so that the storage battery 105 supplies energy to the prompting lamps 10;
a transparent ring 106 is fixedly arranged at one circle of the bottom of the detection box body 1, the transparent ring 106 is connected with the detection box body 1 through an adhesive, the transparent ring 106 is made of transparent PVC (polyvinyl chloride) and is 5mm high, and therefore the light of the prompting lamp 10 can be conveniently emitted outwards under the action of the transparent ring 106, and the medical staff can conveniently check the transparent ring;
the detection composite pad 5 consists of a mouse anti-human supar monoclonal antibody 1-nano fluorescent microsphere compound and a rabbit anti-chicken IgY-nano fluorescent microsphere compound;
in conclusion, through the cooperation effect of imbibition pad 3, imbibition ring 4, detect compound pad 5, sample pad 6 and NC membrane 7, not only can be convenient for detect liquid contact transmission, reach the short-term test mesh, the effectual emergence of avoiding invalid detection, cooperate first conductive terminal 8 in addition, the effect of second conductive terminal 9 and warning light 10, also can realize that liquid is electrically conductive, reach the mesh of triggering warning light 10 circular telegram and lighting, backflow cover 103 and drip hole 104 have also made things convenient for the operation in addition, be convenient for subsequent observation.
Claims (6)
1. The convenient detection device for the chronic kidney disease marker supar is characterized by comprising a detection box body, a detection groove, a liquid absorption pad, a liquid absorption ring, a detection composite pad, a sample pad, an NC (numerical control) membrane, a first conductive terminal, a second conductive terminal and a prompting lamp, wherein the detection box body is cylindrical, the detection groove is positioned in the detection box body, the liquid absorption pad is fixedly arranged at the bottom end in the detection box body, the liquid absorption ring is fixedly arranged on the periphery of the top of the liquid absorption pad, the detection composite pad is fixedly arranged on the inner wall of the liquid absorption ring, the sample pad is fixedly arranged on the inner wall of the detection composite pad, the NC membrane is fixedly arranged on the tops of the detection composite pad and the sample pad, the first conductive terminal is fixedly arranged on the left side of the lower end in the detection box body, the second conductive terminal is fixedly arranged on the right side of the lower end in the detection box body, the first conductive terminal and the second conductive terminal are respectively in liquid absorption contact with the liquid absorption pad in a laminating manner, the prompting lamp is fixedly arranged at the bottom end in the detection box body, the prompting lamp is fixedly arranged on the bottom end in the detection box body, the first conductive terminal is fixedly connected with the first conductive terminal through a conducting wire, a transparent ring is further connected with the detection box body through a detection composite supar compound for detecting rabbit anti-rabbit anti-human-rabbit anti-rabbit nano fluorescent composite compound.
2. The convenient chronic kidney disease marker supar detection device as claimed in claim 1, wherein a box cover is further sleeved on the top of the detection box body, and the box cover and the detection box body are rotatably connected through internal and external threads.
3. The convenient chronic kidney disease marker subpar detection device as claimed in claim 2, wherein an identification slot is further fixedly arranged at the top of the box cover, and the identification slot and the box cover are integrally formed.
4. The convenient chronic kidney disease marker subpar detection device as claimed in claim 1, wherein a backflow cover is fixedly arranged at the upper end inside the detection box body, and the backflow cover and the detection box body are connected by adopting an adhesive.
5. The convenient chronic kidney disease marker subpar detection device as claimed in claim 4, wherein a drip hole is further formed in the middle end of the interior of the backflow cover, and the drip hole is a circular through hole.
6. The convenient chronic kidney disease marker subpar detection device as claimed in claim 1, wherein a storage battery is further arranged on one side of the lower end of the detection box body, the storage battery and the detection box body are connected through a buckle, and the positive electrode and the negative electrode of the storage battery are respectively connected with the second conductive terminal and the indicator light through leads.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910619833.9A CN110208551B (en) | 2019-07-10 | 2019-07-10 | Convenient detection device for chronic kidney disease marker supar |
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| CN201910619833.9A CN110208551B (en) | 2019-07-10 | 2019-07-10 | Convenient detection device for chronic kidney disease marker supar |
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| CN110208551A CN110208551A (en) | 2019-09-06 |
| CN110208551B true CN110208551B (en) | 2023-01-03 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| GB2342443A (en) * | 1998-10-02 | 2000-04-12 | Abp Diagnostics Limited | Immunoassay device |
| US8101429B2 (en) * | 2003-06-03 | 2012-01-24 | Siemens Healthcare Diagnostics Inc. | Native analyte as a reference in lateral flow assays |
| CN102124341A (en) * | 2008-06-04 | 2011-07-13 | Ds基因组公司 | Rapid detection of post-vaccination antibody response |
| CN202442747U (en) * | 2012-02-15 | 2012-09-19 | 赵舜培 | Water detector |
| CN108303550A (en) * | 2018-02-09 | 2018-07-20 | 广东优尼德生物科技有限公司 | A kind of the spot gold diafiltration kit and its quantitative detecting method of detection adiponectin |
| CN208568802U (en) * | 2018-07-02 | 2019-03-01 | 上海凯璟生物科技有限公司 | A kind of chronic kidney disease marker suPAR rapid detection card |
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