CN110201021A - A kind of broccoli seed extraction process - Google Patents
A kind of broccoli seed extraction process Download PDFInfo
- Publication number
- CN110201021A CN110201021A CN201910435329.3A CN201910435329A CN110201021A CN 110201021 A CN110201021 A CN 110201021A CN 201910435329 A CN201910435329 A CN 201910435329A CN 110201021 A CN110201021 A CN 110201021A
- Authority
- CN
- China
- Prior art keywords
- solution
- broccoli seed
- broccoli
- extraction process
- centrifugation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 title claims abstract description 145
- 235000017647 Brassica oleracea var italica Nutrition 0.000 title claims abstract description 145
- 240000003259 Brassica oleracea var. botrytis Species 0.000 title claims abstract description 135
- 238000000605 extraction Methods 0.000 title claims abstract description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 50
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000007788 liquid Substances 0.000 claims abstract description 46
- 239000000843 powder Substances 0.000 claims abstract description 37
- 238000005119 centrifugation Methods 0.000 claims abstract description 30
- 235000019441 ethanol Nutrition 0.000 claims abstract description 24
- 239000012153 distilled water Substances 0.000 claims abstract description 21
- 238000001914 filtration Methods 0.000 claims abstract description 20
- 102000004190 Enzymes Human genes 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 19
- 238000002156 mixing Methods 0.000 claims abstract description 16
- 230000007062 hydrolysis Effects 0.000 claims abstract description 14
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 14
- 230000004927 fusion Effects 0.000 claims abstract description 8
- 238000003801 milling Methods 0.000 claims abstract description 8
- 238000005406 washing Methods 0.000 claims abstract description 7
- 238000002390 rotary evaporation Methods 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 14
- 244000308180 Brassica oleracea var. italica Species 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 7
- 238000005516 engineering process Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 125
- 238000006243 chemical reaction Methods 0.000 description 10
- 239000012141 concentrate Substances 0.000 description 6
- 239000011259 mixed solution Substances 0.000 description 6
- 238000001704 evaporation Methods 0.000 description 5
- 230000008020 evaporation Effects 0.000 description 5
- 230000000415 inactivating effect Effects 0.000 description 5
- 239000000284 extract Substances 0.000 description 3
- -1 glucosinolates compound Chemical class 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000219198 Brassica Species 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- SUVMJBTUFCVSAD-UHFFFAOYSA-N sulforaphane Chemical compound CS(=O)CCCCN=C=S SUVMJBTUFCVSAD-UHFFFAOYSA-N 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- SUVMJBTUFCVSAD-JTQLQIEISA-N 4-Methylsulfinylbutyl isothiocyanate Natural products C[S@](=O)CCCCN=C=S SUVMJBTUFCVSAD-JTQLQIEISA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 241001674939 Caulanthus Species 0.000 description 1
- 241000732800 Cymbidium Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- NMOJAXCSURVGEY-UHFFFAOYSA-N N#CC#N.[S] Chemical compound N#CC#N.[S] NMOJAXCSURVGEY-UHFFFAOYSA-N 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 241000220259 Raphanus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- ZFLXCZJBYSPSKU-CRHREIPCSA-N [(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] (e,1e)-5-methylsulfinyl-n-sulfooxypent-4-enimidothioate Chemical compound CS(=O)\C=C\CC\C(=N/OS(O)(=O)=O)S[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O ZFLXCZJBYSPSKU-CRHREIPCSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010352 biotechnological method Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- ZFLXCZJBYSPSKU-YYSSDTNZSA-N glucoraphenin Natural products C[S@](=O)C=CCCC(=NOS(=O)(=O)O)S[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O ZFLXCZJBYSPSKU-YYSSDTNZSA-N 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 230000024053 secondary metabolic process Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229960005559 sulforaphane Drugs 0.000 description 1
- 235000015487 sulforaphane Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of broccoli seed extraction processes, comprising the following steps: S1 crushes milling: choosing excellent broccoli seed, is put into pulverizer crushing-；S2 adds water to neutralize: broccoli seed powder and distilled water are proportionally added in container；Ultrasonic hydrolysis: S3 will be hydrolyzed in the solution being placed under room temperature again degree of being placed in water-bath；S4, centrifugation mixing: the ethyl alcohol that processed solution is added to inactivates black meson enzyme；Filtration washing: S5 solution after centrifugation is placed on filter and is filtered；Solid-liquid fusion: dregs are merged liquid and clear liquid merge by S6；S7 adds water to be centrifuged, and prepares broccoli seed extracting solution: solution after centrifugation is placed in Rotary Evaporators；By way of ratio, it can be processed in batches broccoli seed, avoid the broccoli seed that can only handle fraction in actual conditions, present invention reduces the production costs of factory, and also the cost for making broccoli seed extracting solution of progress is reduced.
Description
Technical field
The present invention relates to broccoli seed extractive technique field more particularly to a kind of broccoli seed extraction processes.
Background technique
Broccoli, broccoli are commonly called as broccoli, and Appennin sausage is Vegetables.It is generally called Broccoliflorets, it is also referred to as western
Orchid, biennial herb plant are a kind of mutation of wild cabbage, and leaf is big, and stem top forms loose bouquet, green or purple green
The small bud of color, surface is not closely located together, the small bouquet of each life in the top of side shoot, broccoli also known as stem cabbage, broccoli, green flower
Dish, using green bouquet as a mutation of product in Cruciferae Brassica genus brassica specie.The meaning for originating in Europe Mediterranean is big
A sharp band, is passed to China at the end of the 19th century.
Broccoli ascorbic acid rich in, can enhance the detoxification ability of liver, improve immunity of organisms, and wherein one
Quantitative flavonoid substances then have the function of adjusting and prevention to hypertension, heart disease, meanwhile, broccoli belongs to high microsteping vegetable
Dish can be effectively reduced absorption of the stomach to glucose, and then reduce blood glucose, effectively control the state of an illness of diabetes, wherein broccoli
With other crucifers contain family it is relatively unique, be known as glucosinolates compound secondary metabolism, glucoraphenin
For glucosinolates compound important in broccoli, the different sulphur cyanogen of antitumaous effect, radish sulphur are hydrolyzed to form for myrosin
Alkane is then representative most outstanding in isothiocyanic acid salt compound, and broccoli is to contain the most abundant plant of sulforaphane.
Existing broccoli seed when extracting broccoli seed extracting solution, can only for fraction broccoli seed into
Row processing, so crushing extraction can not be carried out for the blue flower seed in large batch of west in actual conditions, and in broccoli seed
When extracting broccoli seed extracting solution, wherein the utilization rate of broccoli seed is very low, results in the broccoli of identical quantity in this way
Seed amount can not extract more broccoli seed extracting solutions, therefore we need to propose that one kind can extract west on a large scale
The method of cymbidium seed extracting solution.
Summary of the invention
Technical problems based on background technology, the invention proposes a kind of broccoli seed extraction processes.
A kind of broccoli seed extraction process proposed by the present invention, comprising the following steps:
S1 crushes milling: choosing excellent broccoli seed, is put into pulverizer and crushes 10-20min, then crushing
Broccoli seed powder afterwards screens and intercepts foreign matter therein, later that broccoli seed powder indwelling is spare；
S3, ultrasonic hydrolysis: the solution being placed under room temperature is placed in again in 40 degree of water-baths, 4h is hydrolyzed, later will be through
The solution for crossing hydrolysis is placed in vibration processing 20min under ultrasonic wave；
Centrifugation mixing: the ethyl alcohol that processed solution is added to 1:2 is inactivated black meson enzyme, then put at normal temperature by S4
10min is set, solution is placed into centrifuge later and is centrifuged；
S6, solid-liquid fusion: merging liquid for dregs and clear liquid merge, 10min in the environment of being then placed into 40 degree, then will
Mixed solution is placed into centrifuge herein and is centrifuged；
S7 adds water to be centrifuged, and prepares broccoli seed extracting solution: solution after centrifugation is placed in Rotary Evaporators,
Rotary evaporation is carried out, and will be contracted by the solution addition distilled water after rotary evaporation to get to broccoli seed water concentrate
Liquid；
It preferably, will be interior with 80-100 revs/min of progress powder by preferred broccoli seed transfer pulverizer in the S1
It is broken, it is ensured that broccoli seed can be crushed thoroughly, and the powder after crushing is selected by 50 meshes, obtains broccoli
Seed powder.
Preferably, solution is subjected to triple mixing in the S2, the solution first passes around 40-60 revs/min of revolving speed progress
Stirring, the solution are stirred secondary by 70-80 revs/min of revolving speed, the solution finally pass through 90-100 revs/min of revolving speed into
Row stirring.
Preferably, the filter hole of filter is 30 mesh in the S5, and the dregs after the filtering have to be placed in nothing
Among the environment of bacterium.
Preferably, the clear liquid in the S6 is the liquid of solution for the first time after centrifugation.
Preferably, solution is placed with rotary evaporator in the S7, the motor rotary speed of the rotary evaporator is
1000-1300 revs/min.
Preferably, the distilled water of the solution addition 1:3 in the S7 by rotary evaporation is neutralized.
Beneficial effects of the present invention are as follows: by way of ratio, can be processed in batches broccoli seed, avoid practical feelings
The broccoli seed that fraction can only be handled in condition improves the quantity of processing broccoli seed, improves the work effect of engineering
Rate reduces the production cost of factory, and also the cost for making broccoli seed extracting solution of progress is reduced, by multiple
Metering method and metering method use, so as to so that the extracting solution of broccoli seed can increase, letter speech and know and be exactly
Least broccoli seed quantity has been used, and more broccoli seed extracting solutions can be obtained, has reduced costs, improves life
Produce efficiency, the present invention by biotechnological method in a short time, meet the demand commercially produced, be enterprise scale,
Industrialization extracts broccoli seed extracting solution and provides technical support.
Detailed description of the invention
Fig. 1 is the preparation flow figure of broccoli seed extracting solution.
Specific embodiment
Combined with specific embodiments below the present invention is made further to explain.
Embodiment 1
S1 crushes milling: choosing excellent broccoli seed, is put into pulverizer and crushes 10-20min, then crushing
Broccoli seed powder afterwards screens and intercepts foreign matter therein, later that broccoli seed powder indwelling is spare；
S2 adds water to neutralize: broccoli seed powder and distilled water being added in container according to the ratio of 1:10, and passed through
Repeatedly stirring is crossed, the solution after stirring is placed in 20min under room temperature；
S3, ultrasonic hydrolysis: the solution being placed under room temperature is placed in again in 40 degree of water-baths, 4h is hydrolyzed, later will be through
The solution for crossing hydrolysis is placed in vibration processing 20min under ultrasonic wave；
Centrifugation mixing: the ethyl alcohol that processed solution is added to 1:2 is inactivated black meson enzyme, then put at normal temperature by S4
Set 5min, it is 52% that wherein ethyl alcohol, which inactivates the extent of reaction of black meson and solution, later by solution be placed into centrifuge carry out from
The heart；
S5, filtration washing: solution after centrifugation being placed on filter and is filtered, and the dregs after filtering are put
It is placed in 10min in normal temperature environment, then rinses dregs 3 times respectively with the pure water of 1:0.5；
S6, solid-liquid fusion: merging liquid for dregs and clear liquid merge, 10min in the environment of being then placed into 40 degree, then will
Mixed solution is placed into centrifuge herein and is centrifuged；
S7 adds water to be centrifuged, and prepares broccoli seed extracting solution: solution after centrifugation is placed in Rotary Evaporators,
Rotary evaporation is carried out, and will be contracted by the solution addition distilled water after rotary evaporation to get to broccoli seed water concentrate
Liquid.
2, a kind of broccoli seed extraction process according to claim 1, which is characterized in that will pass through in the S1
It is crushed in preferred broccoli seed transfer pulverizer with 80-100 revs/min, it is ensured that broccoli seed can obtain thoroughly
Crushing, and crush after powder by 50 meshes choosing, obtain broccoli seed powder.
3, a kind of broccoli seed extraction process according to claim 1, which is characterized in that by solution in the S2
Triple mixing is carried out, the solution first passes around 40-60 revs/min of revolving speed and is stirred, and the solution passes through revolving speed 70- secondary
80 revs/min are stirred, and the solution finally passes through 90-100 revs/min of revolving speed and is stirred.
4, a kind of broccoli seed extraction process according to claim 1, which is characterized in that filter in the S5
Filter hole be 30 mesh, the dregs after the filtering have to be placed among sterile environment.
5, a kind of broccoli seed extraction process according to claim 1, which is characterized in that the clear liquid in the S6
For the liquid of solution for the first time after centrifugation.
6, a kind of broccoli seed extraction process according to claim 1, which is characterized in that by solution in the S7
It places with rotary evaporator, the motor rotary speed of the rotary evaporator is 1000-1300 revs/min.
7, a kind of broccoli seed extraction process according to claim 1, which is characterized in that through overwinding in the S7
The distilled water for turning the solution addition 1:3 of evaporation is neutralized.
The solution for being added to ethyl alcohol and inactivating black meson enzyme is placed in room temperature in S4, it is black that the time is that 5min ethyl alcohol inactivates
The extent of reaction of meson enzyme and solution is 52%, and the concentration of broccoli seed extracting solution is 30%.
Embodiment 2
S1 crushes milling: choosing excellent broccoli seed, is put into pulverizer and crushes 10-20min, then crushing
Broccoli seed powder afterwards screens and intercepts foreign matter therein, later that broccoli seed powder indwelling is spare；
S2 adds water to neutralize: broccoli seed powder and distilled water being added in container according to the ratio of 1:10, and passed through
Repeatedly stirring is crossed, the solution after stirring is placed in 20min under room temperature；
S3, ultrasonic hydrolysis: the solution being placed under room temperature is placed in again in 40 degree of water-baths, 4h is hydrolyzed, later will be through
The solution for crossing hydrolysis is placed in vibration processing 20min under ultrasonic wave；
Centrifugation mixing: the ethyl alcohol that processed solution is added to 1:2 is inactivated black meson enzyme, then put at normal temperature by S4
Set 7min, it is 73% that wherein ethyl alcohol, which inactivates the extent of reaction of black meson and solution, later by solution be placed into centrifuge carry out from
The heart；
S5, filtration washing: solution after centrifugation being placed on filter and is filtered, and the dregs after filtering are put
It is placed in 10min in normal temperature environment, then rinses dregs 3 times respectively with the pure water of 1:0.5；
S6, solid-liquid fusion: merging liquid for dregs and clear liquid merge, 10min in the environment of being then placed into 40 degree, then will
Mixed solution is placed into centrifuge herein and is centrifuged；
S7 adds water to be centrifuged, and prepares broccoli seed extracting solution: solution after centrifugation is placed in Rotary Evaporators,
Rotary evaporation is carried out, and will be contracted by the solution addition distilled water after rotary evaporation to get to broccoli seed water concentrate
Liquid.
2, a kind of broccoli seed extraction process according to claim 1, which is characterized in that will pass through in the S1
It is crushed in preferred broccoli seed transfer pulverizer with 80-100 revs/min, it is ensured that broccoli seed can obtain thoroughly
Crushing, and crush after powder by 50 meshes choosing, obtain broccoli seed powder.
3, a kind of broccoli seed extraction process according to claim 1, which is characterized in that by solution in the S2
Triple mixing is carried out, the solution first passes around 40-60 revs/min of revolving speed and is stirred, and the solution passes through revolving speed 70- secondary
80 revs/min are stirred, and the solution finally passes through 90-100 revs/min of revolving speed and is stirred.
4, a kind of broccoli seed extraction process according to claim 1, which is characterized in that filter in the S5
Filter hole be 30 mesh, the dregs after the filtering have to be placed among sterile environment.
5, a kind of broccoli seed extraction process according to claim 1, which is characterized in that the clear liquid in the S6
For the liquid of solution for the first time after centrifugation.
6, a kind of broccoli seed extraction process according to claim 1, which is characterized in that by solution in the S7
It places with rotary evaporator, the motor rotary speed of the rotary evaporator is 1000-1300 revs/min.
7, a kind of broccoli seed extraction process according to claim 1, which is characterized in that through overwinding in the S7
The distilled water for turning the solution addition 1:3 of evaporation is neutralized.
The solution for being added to ethyl alcohol and inactivating black meson enzyme is placed in room temperature in S4, it is black that the time is that 7min ethyl alcohol inactivates
The extent of reaction of meson enzyme and solution is 73%, and the concentration of broccoli seed extracting solution is 41%.
Embodiment 3
S1 crushes milling: choosing excellent broccoli seed, is put into pulverizer and crushes 10-20min, then crushing
Broccoli seed powder afterwards screens and intercepts foreign matter therein, later that broccoli seed powder indwelling is spare；
S2 adds water to neutralize: broccoli seed powder and distilled water being added in container according to the ratio of 1:10, and passed through
Repeatedly stirring is crossed, the solution after stirring is placed in 20min under room temperature；
S3, ultrasonic hydrolysis: the solution being placed under room temperature is placed in again in 40 degree of water-baths, 4h is hydrolyzed, later will be through
The solution for crossing hydrolysis is placed in vibration processing 20min under ultrasonic wave；
Centrifugation mixing: the ethyl alcohol that processed solution is added to 1:2 is inactivated black meson enzyme, then put at normal temperature by S4
Set 8min, it is 80% that wherein ethyl alcohol, which inactivates the extent of reaction of black meson and solution, later by solution be placed into centrifuge carry out from
The heart；
S5, filtration washing: solution after centrifugation being placed on filter and is filtered, and the dregs after filtering are put
It is placed in 10min in normal temperature environment, then rinses dregs 3 times respectively with the pure water of 1:0.5；
S6, solid-liquid fusion: merging liquid for dregs and clear liquid merge, 10min in the environment of being then placed into 40 degree, then will
Mixed solution is placed into centrifuge herein and is centrifuged；
S7 adds water to be centrifuged, and prepares broccoli seed extracting solution: solution after centrifugation is placed in Rotary Evaporators,
Rotary evaporation is carried out, and will be contracted by the solution addition distilled water after rotary evaporation to get to broccoli seed water concentrate
Liquid.
2, a kind of broccoli seed extraction process according to claim 1, which is characterized in that will pass through in the S1
It is crushed in preferred broccoli seed transfer pulverizer with 80-100 revs/min, it is ensured that broccoli seed can obtain thoroughly
Crushing, and crush after powder by 50 meshes choosing, obtain broccoli seed powder.
3, a kind of broccoli seed extraction process according to claim 1, which is characterized in that by solution in the S2
Triple mixing is carried out, the solution first passes around 40-60 revs/min of revolving speed and is stirred, and the solution passes through revolving speed 70- secondary
80 revs/min are stirred, and the solution finally passes through 90-100 revs/min of revolving speed and is stirred.
4, a kind of broccoli seed extraction process according to claim 1, which is characterized in that filter in the S5
Filter hole be 30 mesh, the dregs after the filtering have to be placed among sterile environment.
5, a kind of broccoli seed extraction process according to claim 1, which is characterized in that the clear liquid in the S6
For the liquid of solution for the first time after centrifugation.
6, a kind of broccoli seed extraction process according to claim 1, which is characterized in that by solution in the S7
It places with rotary evaporator, the motor rotary speed of the rotary evaporator is 1000-1300 revs/min.
7, a kind of broccoli seed extraction process according to claim 1, which is characterized in that through overwinding in the S7
The distilled water for turning the solution addition 1:3 of evaporation is neutralized.
The solution for being added to ethyl alcohol and inactivating black meson enzyme is placed in room temperature in S4, it is black that the time is that 8min ethyl alcohol inactivates
The extent of reaction of meson enzyme and solution is 80%, and the concentration of broccoli seed extracting solution is 55%
Embodiment 4
S1 crushes milling: choosing excellent broccoli seed, is put into pulverizer and crushes 10-20min, then crushing
Broccoli seed powder afterwards screens and intercepts foreign matter therein, later that broccoli seed powder indwelling is spare；
S2 adds water to neutralize: broccoli seed powder and distilled water being added in container according to the ratio of 1:10, and passed through
Repeatedly stirring is crossed, the solution after stirring is placed in 20min under room temperature；
S3, ultrasonic hydrolysis: the solution being placed under room temperature is placed in again in 40 degree of water-baths, 4h is hydrolyzed, later will be through
The solution for crossing hydrolysis is placed in vibration processing 20min under ultrasonic wave；
Centrifugation mixing: the ethyl alcohol that processed solution is added to 1:2 is inactivated black meson enzyme, then put at normal temperature by S4
Set 9min, it is 86% that wherein ethyl alcohol, which inactivates the extent of reaction of black meson and solution, later by solution be placed into centrifuge carry out from
The heart；
S5, filtration washing: solution after centrifugation being placed on filter and is filtered, and the dregs after filtering are put
It is placed in 10min in normal temperature environment, then rinses dregs 3 times respectively with the pure water of 1:0.5；
S6, solid-liquid fusion: merging liquid for dregs and clear liquid merge, 10min in the environment of being then placed into 40 degree, then will
Mixed solution is placed into centrifuge herein and is centrifuged；
S7 adds water to be centrifuged, and prepares broccoli seed extracting solution: solution after centrifugation is placed in Rotary Evaporators,
Rotary evaporation is carried out, and will be contracted by the solution addition distilled water after rotary evaporation to get to broccoli seed water concentrate
Liquid.
2, a kind of broccoli seed extraction process according to claim 1, which is characterized in that will pass through in the S1
It is crushed in preferred broccoli seed transfer pulverizer with 80-100 revs/min, it is ensured that broccoli seed can obtain thoroughly
Crushing, and crush after powder by 50 meshes choosing, obtain broccoli seed powder.
3, a kind of broccoli seed extraction process according to claim 1, which is characterized in that by solution in the S2
Triple mixing is carried out, the solution first passes around 40-60 revs/min of revolving speed and is stirred, and the solution passes through revolving speed 70- secondary
80 revs/min are stirred, and the solution finally passes through 90-100 revs/min of revolving speed and is stirred.
4, a kind of broccoli seed extraction process according to claim 1, which is characterized in that filter in the S5
Filter hole be 30 mesh, the dregs after the filtering have to be placed among sterile environment.
5, a kind of broccoli seed extraction process according to claim 1, which is characterized in that the clear liquid in the S6
For the liquid of solution for the first time after centrifugation.
6, a kind of broccoli seed extraction process according to claim 1, which is characterized in that by solution in the S7
It places with rotary evaporator, the motor rotary speed of the rotary evaporator is 1000-1300 revs/min.
7, a kind of broccoli seed extraction process according to claim 1, which is characterized in that through overwinding in the S7
The distilled water for turning the solution addition 1:3 of evaporation is neutralized.
The solution for being added to ethyl alcohol and inactivating black meson enzyme is placed in room temperature in S4, it is black that the time is that 9min ethyl alcohol inactivates
The extent of reaction of meson enzyme and solution is 86%, and the concentration of broccoli seed extracting solution is 67%.
Embodiment 5
S1 crushes milling: choosing excellent broccoli seed, is put into pulverizer and crushes 10-20min, then crushing
Broccoli seed powder afterwards screens and intercepts foreign matter therein, later that broccoli seed powder indwelling is spare；
S2 adds water to neutralize: broccoli seed powder and distilled water being added in container according to the ratio of 1:10, and passed through
Repeatedly stirring is crossed, the solution after stirring is placed in 20min under room temperature；
S3, ultrasonic hydrolysis: the solution being placed under room temperature is placed in again in 40 degree of water-baths, 4h is hydrolyzed, later will be through
The solution for crossing hydrolysis is placed in vibration processing 20min under ultrasonic wave；
Centrifugation mixing: the ethyl alcohol that processed solution is added to 1:2 is inactivated black meson enzyme, then put at normal temperature by S4
Set 10min, it is 79% that wherein ethyl alcohol, which inactivates the extent of reaction of black meson and solution, later by solution be placed into centrifuge carry out from
The heart；
S5, filtration washing: solution after centrifugation being placed on filter and is filtered, and the dregs after filtering are put
It is placed in 10min in normal temperature environment, then rinses dregs 3 times respectively with the pure water of 1:0.5；
S6, solid-liquid fusion: merging liquid for dregs and clear liquid merge, 10min in the environment of being then placed into 40 degree, then will
Mixed solution is placed into centrifuge herein and is centrifuged；
S7 adds water to be centrifuged, and prepares broccoli seed extracting solution: solution after centrifugation is placed in Rotary Evaporators,
Rotary evaporation is carried out, and will be contracted by the solution addition distilled water after rotary evaporation to get to broccoli seed water concentrate
Liquid.
2, a kind of broccoli seed extraction process according to claim 1, which is characterized in that will pass through in the S1
It is crushed in preferred broccoli seed transfer pulverizer with 80-100 revs/min, it is ensured that broccoli seed can obtain thoroughly
Crushing, and crush after powder by 50 meshes choosing, obtain broccoli seed powder.
3, a kind of broccoli seed extraction process according to claim 1, which is characterized in that by solution in the S2
Triple mixing is carried out, the solution first passes around 40-60 revs/min of revolving speed and is stirred, and the solution passes through revolving speed 70- secondary
80 revs/min are stirred, and the solution finally passes through 90-100 revs/min of revolving speed and is stirred.
4, a kind of broccoli seed extraction process according to claim 1, which is characterized in that filter in the S5
Filter hole be 30 mesh, the dregs after the filtering have to be placed among sterile environment.
5, a kind of broccoli seed extraction process according to claim 1, which is characterized in that the clear liquid in the S6
For the liquid of solution for the first time after centrifugation.
6, a kind of broccoli seed extraction process according to claim 1, which is characterized in that by solution in the S7
It places with rotary evaporator, the motor rotary speed of the rotary evaporator is 1000-1300 revs/min.
7, a kind of broccoli seed extraction process according to claim 1, which is characterized in that through overwinding in the S7
The distilled water for turning the solution addition 1:3 of evaporation is neutralized.
The solution for being added to ethyl alcohol and inactivating black meson enzyme is placed in room temperature in S4, it is black that the time is that 10min ethyl alcohol inactivates
The extent of reaction of meson enzyme and solution is 79%, and the concentration of broccoli seed extracting solution is 63%.
It follows that ethyl alcohol inactivates the reactivity of black meson enzyme and solution as 86% after using embodiment 4 to handle, broccoli
The concentration of seed extracting solution is 67%, is not set forth in detail here.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (7)
1. a kind of reparation technology of broccoli seed health care product, which comprises the following steps:
S1 crushes milling: choosing excellent broccoli seed, is put into pulverizer and crushes 10-20min, then after crushing
Broccoli seed powder screens and intercepts foreign matter therein, later that broccoli seed powder indwelling is spare；
S2 adds water to neutralize: broccoli seed powder and distilled water being added in container according to the ratio of 1:10, and through excessive
Solution after stirring is placed in 20min under room temperature by secondary stirring；
S3, ultrasonic hydrolysis: the solution being placed under room temperature is placed in again in 40 degree of water-baths, 4h is hydrolyzed, and will pass through water later
The solution of solution is placed in vibration processing 20min under ultrasonic wave；
Centrifugation mixing: the ethyl alcohol that processed solution is added to 1:2 is inactivated black meson enzyme, then places 5- at normal temperature by S4
Solution is placed into centrifuge later and is centrifuged by 10min；
S5, filtration washing: solution after centrifugation being placed on filter and is filtered, and the dregs after filtering are placed in
Then 10min in normal temperature environment rinses dregs 3 times with the pure water of 1:0.5 respectively；
S6, solid-liquid fusion: merging liquid for dregs and clear liquid merge, 10min in the environment of being then placed into 40 degree, then will mixing
Solution be placed into centrifuge herein and be centrifuged；
S7 adds water to be centrifuged, and prepares broccoli seed extracting solution: solution after centrifugation being placed in Rotary Evaporators, is carried out
Rotary evaporation, and the solution after process rotary evaporation is added into distilled water to get broccoli seed water extracting liquid is arrived.
2. a kind of broccoli seed extraction process according to claim 1, which is characterized in that will be by preferred in the S1
Broccoli seed transfer pulverizer in crushed with 80-100 revs/min, it is ensured that broccoli seed can obtain thorough powder
It is broken, and the powder after crushing is selected by 50 meshes, obtains broccoli seed powder.
3. a kind of broccoli seed extraction process according to claim 1, which is characterized in that carry out solution in the S2
Triple mixing, the solution first pass around 40-60 revs/min of revolving speed and are stirred, the solution it is secondary by revolving speed 70-80 turn/
Divide and be stirred, the solution finally passes through 90-100 revs/min of revolving speed and is stirred.
4. a kind of broccoli seed extraction process according to claim 1, which is characterized in that the mistake of filter in the S5
Filter opening is 30 mesh, and the dregs after the filtering have to be placed among sterile environment.
5. a kind of broccoli seed extraction process according to claim 1, which is characterized in that the clear liquid in the S6 is molten
The liquid of liquid for the first time after centrifugation.
6. a kind of broccoli seed extraction process according to claim 1, which is characterized in that place solution in the S7
In rotary evaporator, the motor rotary speed of the rotary evaporator is 1000-1300 revs/min.
7. a kind of broccoli seed extraction process according to claim 1, which is characterized in that steamed in the S7 by rotation
The distilled water of the solution addition 1:3 of hair is neutralized.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910435329.3A CN110201021A (en) | 2019-05-23 | 2019-05-23 | A kind of broccoli seed extraction process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910435329.3A CN110201021A (en) | 2019-05-23 | 2019-05-23 | A kind of broccoli seed extraction process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110201021A true CN110201021A (en) | 2019-09-06 |
Family
ID=67788395
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910435329.3A Pending CN110201021A (en) | 2019-05-23 | 2019-05-23 | A kind of broccoli seed extraction process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110201021A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114916670A (en) * | 2022-05-26 | 2022-08-19 | 安徽本森堂生物科技有限公司 | Meal replacement powder assisting in reducing blood sugar and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102898341A (en) * | 2012-10-26 | 2013-01-30 | 贵州大学 | Extracting and purifying method of high-purity sulforaphane |
-
2019
- 2019-05-23 CN CN201910435329.3A patent/CN110201021A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102898341A (en) * | 2012-10-26 | 2013-01-30 | 贵州大学 | Extracting and purifying method of high-purity sulforaphane |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114916670A (en) * | 2022-05-26 | 2022-08-19 | 安徽本森堂生物科技有限公司 | Meal replacement powder assisting in reducing blood sugar and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101878845B (en) | Medlar coffee | |
| CN101491326B (en) | Perilla sauce and preparation technique thereof | |
| CN110759797A (en) | Plant fermentation extract for improving peanut planting soil and preparation method thereof | |
| CN101138430B (en) | Method for producing red date clarified juice | |
| CN102965241A (en) | Processing method of blueberry and honeysuckle fruit wine | |
| CN102845599B (en) | The reuse method of root, stem, leaf and seed Chinese medicine slag | |
| CN103989715B (en) | Series of products and method are prepared using pine needle | |
| CN104222495B (en) | A kind of method utilizing agricultural crop straw to prepare Herbivore feed | |
| CN109851606A (en) | A method of extracting anthocyanidin from Black Box Tracing | |
| CN104351892A (en) | Production method for radix puerariae beverage | |
| CN106834014A (en) | A kind of selenium-rich waxberry health-care wine and preparation method thereof | |
| CN102657306A (en) | Fine dried Japanese premna herb noodles | |
| CN104041901B (en) | Peony peptide drink and preparation method of peony peptide drink | |
| CN110201021A (en) | A kind of broccoli seed extraction process | |
| CN103222581A (en) | Bean dreg carrot steamed bun and preparation method thereof | |
| CN106723026A (en) | A kind of Barley Greeg and its preparation technology | |
| CN108753558B (en) | Preparation method of red yeast rice olive vinegar | |
| CN105660888B (en) | A kind of camellia oil compound product and preparation method thereof | |
| CN1225801A (en) | Compounded Mailusuo and preparation process thereof | |
| CN103494294A (en) | Diversecolor cinquefoil and mulberry leaf sugar-reducing healthcare beverage and preparation method thereof | |
| CN106360172A (en) | Preparation method of fermentation aroma type pueraria lobata, water chestnut and saccharum officinarum composite beverage | |
| CN101878844A (en) | Cynomorium coffee | |
| CN106261273A (en) | A kind of leaf of Moringa blue berry composite fruit juice with blood fat reducing function and preparation method thereof | |
| CN104830594A (en) | Making method for pumpkin health wine | |
| CN104726302B (en) | A kind of persimmon wine and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190906 |