CN110200287A - A kind of oyster polypeptide-nano granules of selenium compound and the preparation method and application thereof with relieving alcoholism and protecting liver effect - Google Patents
A kind of oyster polypeptide-nano granules of selenium compound and the preparation method and application thereof with relieving alcoholism and protecting liver effect Download PDFInfo
- Publication number
- CN110200287A CN110200287A CN201910581455.XA CN201910581455A CN110200287A CN 110200287 A CN110200287 A CN 110200287A CN 201910581455 A CN201910581455 A CN 201910581455A CN 110200287 A CN110200287 A CN 110200287A
- Authority
- CN
- China
- Prior art keywords
- oyster
- selenium
- preparation
- polypeptide
- oyster polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000237502 Ostreidae Species 0.000 title claims abstract description 126
- 235000020636 oyster Nutrition 0.000 title claims abstract description 126
- 230000000694 effects Effects 0.000 title claims abstract description 51
- 239000008187 granular material Substances 0.000 title claims abstract description 48
- 210000004185 liver Anatomy 0.000 title claims abstract description 40
- 229940065287 selenium compound Drugs 0.000 title claims abstract description 39
- 150000003343 selenium compounds Chemical class 0.000 title claims abstract description 39
- 208000007848 Alcoholism Diseases 0.000 title claims abstract description 37
- 201000007930 alcohol dependence Diseases 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 72
- 229920001184 polypeptide Polymers 0.000 claims abstract description 55
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 55
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims abstract description 53
- 239000011669 selenium Substances 0.000 claims abstract description 53
- 229910052711 selenium Inorganic materials 0.000 claims abstract description 51
- 229940091258 selenium supplement Drugs 0.000 claims abstract description 51
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- 102000004190 Enzymes Human genes 0.000 claims abstract description 24
- 108090000790 Enzymes Proteins 0.000 claims abstract description 24
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 22
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 22
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 22
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims abstract description 20
- 229960001471 sodium selenite Drugs 0.000 claims abstract description 20
- 239000011781 sodium selenite Substances 0.000 claims abstract description 20
- 235000015921 sodium selenite Nutrition 0.000 claims abstract description 20
- 235000013372 meat Nutrition 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 13
- 238000004108 freeze drying Methods 0.000 claims abstract description 12
- 238000000502 dialysis Methods 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 11
- 239000006228 supernatant Substances 0.000 claims abstract description 10
- 238000009835 boiling Methods 0.000 claims abstract description 9
- 239000000047 product Substances 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 230000036541 health Effects 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims description 23
- 238000006243 chemical reaction Methods 0.000 claims description 22
- 239000004365 Protease Substances 0.000 claims description 13
- 238000001976 enzyme digestion Methods 0.000 claims description 12
- 108010019160 Pancreatin Proteins 0.000 claims description 10
- 229940055695 pancreatin Drugs 0.000 claims description 8
- 108091005804 Peptidases Proteins 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 7
- 238000005119 centrifugation Methods 0.000 claims description 7
- 235000019419 proteases Nutrition 0.000 claims description 7
- 238000000265 homogenisation Methods 0.000 claims description 6
- 238000009210 therapy by ultrasound Methods 0.000 claims description 6
- 108090000526 Papain Proteins 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 4
- 235000013376 functional food Nutrition 0.000 claims description 4
- 235000019834 papain Nutrition 0.000 claims description 4
- 229940055729 papain Drugs 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 108010004032 Bromelains Proteins 0.000 claims description 2
- NGWKGSCSHDHHAJ-YPFQVHCOSA-N Liquoric acid Chemical compound C1C[C@H](O)C(C)(C)C2CC[C@@]3(C)[C@]4(C)C[C@H]5O[C@@H]([C@](C6)(C)C(O)=O)C[C@@]5(C)[C@@H]6C4=CC(=O)C3[C@]21C NGWKGSCSHDHHAJ-YPFQVHCOSA-N 0.000 claims description 2
- NGWKGSCSHDHHAJ-UHFFFAOYSA-N Liquoric acid Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CC5OC(C(C6)(C)C(O)=O)CC5(C)C6C4=CC(=O)C3C21C NGWKGSCSHDHHAJ-UHFFFAOYSA-N 0.000 claims description 2
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 2
- 238000004458 analytical method Methods 0.000 claims description 2
- 235000019835 bromelain Nutrition 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- SCWQRZHMKQEINE-UHFFFAOYSA-N selenous acid;sodium Chemical compound [Na].O[Se](O)=O SCWQRZHMKQEINE-UHFFFAOYSA-N 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 12
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 abstract description 11
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract description 8
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract description 8
- 238000002604 ultrasonography Methods 0.000 abstract description 5
- 238000003556 assay Methods 0.000 abstract description 4
- 238000001816 cooling Methods 0.000 abstract 1
- 230000000254 damaging effect Effects 0.000 abstract 1
- 230000000087 stabilizing effect Effects 0.000 abstract 1
- 230000001502 supplementing effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 44
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 15
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 15
- 239000008279 sol Substances 0.000 description 14
- 238000007710 freezing Methods 0.000 description 11
- 230000008014 freezing Effects 0.000 description 11
- 239000000463 material Substances 0.000 description 11
- 102000004169 proteins and genes Human genes 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- 238000001035 drying Methods 0.000 description 10
- 239000003795 chemical substances by application Substances 0.000 description 9
- 235000019441 ethanol Nutrition 0.000 description 9
- 238000002156 mixing Methods 0.000 description 9
- 239000000654 additive Substances 0.000 description 8
- 230000000996 additive effect Effects 0.000 description 8
- 235000013339 cereals Nutrition 0.000 description 8
- 231100000753 hepatic injury Toxicity 0.000 description 8
- 230000001681 protective effect Effects 0.000 description 6
- 230000033228 biological regulation Effects 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 230000035484 reaction time Effects 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 4
- 230000009849 deactivation Effects 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 238000002525 ultrasonication Methods 0.000 description 4
- 230000004913 activation Effects 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000000975 bioactive effect Effects 0.000 description 3
- 239000013068 control sample Substances 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 206010067125 Liver injury Diseases 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 229960000074 biopharmaceutical Drugs 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000002270 dispersing agent Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 235000015170 shellfish Nutrition 0.000 description 2
- 239000012798 spherical particle Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- GDSOZVZXVXTJMI-SNAWJCMRSA-N (e)-1-methylbut-1-ene-1,2,4-tricarboxylic acid Chemical compound OC(=O)C(/C)=C(C(O)=O)\CCC(O)=O GDSOZVZXVXTJMI-SNAWJCMRSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 206010015037 epilepsy Diseases 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 230000002443 hepatoprotective effect Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 125000003748 selenium group Chemical group *[Se]* 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
- 229940048086 sodium pyrophosphate Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/65—Addition of, or treatment with, microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Marine Sciences & Fisheries (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention discloses a kind of oyster polypeptide-nano granules of selenium compound and the preparation method and application thereof with relieving alcoholism and protecting liver effect.This method comprises: oyster meat is homogenized, water and organized enzyme, enzymatic hydrolysis is added, boiling water bath, centrifuging and taking supernatant do supernatant liquid cooling, oyster polypeptide powder is obtained, is added to the water, is mixed into solution, sodium selenite solution is added in oyster polypeptide solution, is mixed, while stirring, ascorbic acid solution, water-bath, dialysis is added, functionalized nano selenium sol is obtained, ultrasound, freeze-drying obtains the oyster polypeptide-nano granules of selenium compound with relieving alcoholism and protecting liver effect.Present invention oyster stabilizing polypeptides nanometer selenium, significantly increases the stability of nanometer selenium, can be storage-stable 1 month at 4 DEG C, detects through mtt assay, which can mitigate alcohol or hydrogen peroxide to the damaging action of liver.If converting the product to liver-protecting function and capable of supplementing the health care product of selenium element, it will generate biggish economic results in society.
Description
Technical field
The invention belongs to nanometer selenium nutritional preparations and functional food preparation technical field, and in particular to one kind, which has, relieves the effect of alcohol
Oyster polypeptide-nano granules of selenium compound of protect liver effect and the preparation method and application thereof.
Background technique
Selenium (Selenium, Se) is one of required, indispensable nutrient in organism, is human erythrocyte paddy
The important composition ingredient of the sweet peptide peroxidase of Guang, main function are the synthesis for participating in enzyme, avoid cell membrane excessive oxidation, are protected
Its structure and function are protected, has the function of the multi-biologicals such as anti-oxidant, antitumor, prevention cardiovascular disease, epilepsy.
Nanometer selenium belongs to zeroth order selenium, and general norivalent elements selenium cannot be absorbed by the body utilization, and nanometer selenium is due to its spy
Different nanostructure, can directly be absorbed by the body utilization.Compared with organic selenium, nanometer selenium have better bioavailability and
Higher bioactivity, and there is lower toxicity, it is the minimum selenium form of the toxicity that has been found at present.In addition, receiving
Rice selenium bioactive functions are prominent, have the characteristics such as anti-oxidant, antitumor, protect liver, immunity regulatin remedy, antibacterial, in selenium-supply product
It is had a good application prospect in exploitation and medical field of nanometer technology.The common synthetic method of nanometer selenium has chemical synthesis at present
Method and biological synthesis process, but due to biological synthesis process long preparation period, and the nano granules of selenium formed is larger, the scope of application is small,
It is not used widely.And the red elemental selenium that chemical synthesis can be formed quickly, but it is unstable when the elemental selenium individualism
It is fixed, it is easy aggregation, adjusting control agent is generally needed to be added and increases its stability.Some researches show that some polysaccharide, albumen and surfaces at present
Activating agent (polyvinyl alcohol, lauryl sodium sulfate, sodium carboxymethylcellulose etc.) etc. can be used as the adjusting control agent of nanometer selenium formation.With
The other biologicals macromolecular such as polysaccharide is compared, and protide adjusting control agent has more advantage in terms of biocompatibility and physiological activity.But
The nano granules of selenium compound that adjusting control agent is formed is added to be easy to lead to whole due to nanometer selenium surface is unevenly distributed because of adjusting control agent
The storage-stable of grain is poor.In addition, the functional activity of research multi-focus selenium itself, and adjusting control agent itself is raw to nano granules of selenium
It is less to manage active synergistic function correlative study.
Oyster (Oyster) is known as " marine milk ", is that a kind of global shellfish and China famous four cultivate greatly
One of economic shellfish, is distributed widely in the ground such as Liaoning, Hebei, Shandong, Jiangsu, Zhejiang, Guangdong, Guangxi, Hainan and China defends
First medicinal and health care functional food of life portion approval.The content of protein is higher in oyster, is to prepare bioactivity
The very good material of peptide.The active peptide content being naturally occurring in marine organisms is less, and extracts difficult, it is difficult to mass production；
Chemically synthesized polypeptide is time-consuming and laborious, expensive, therefore people more and more utilize the method for enzymatic hydrolysis from marine protein
Active peptide is extracted in matter.Food industry to the Study on Physiological Activity of oyster peptide be concentrated mainly on anti-angiocardiopathy and anticoagulation,
Reducing blood lipid, antithrombotic, raising body's immunity etc..And according to its amino acid and minerals compositing characteristic, utilize biology
The research that zymolysis technique preparation has both the oyster peptide of relieving alcoholism and protecting liver ability and regulation nanometer selenium Forming ability never has been reported that.
Summary of the invention
In order to overcome deficiencies of the prior art, the object of the present invention is to provide one kind to have relieving alcoholism and protecting liver effect
Oyster polypeptide-nano granules of selenium compound preparation method and application.
The primary purpose of the present invention is that providing a seed oyster polypeptide-nano granules of selenium compound.Nanometer selenium technology of preparing
There is extensive use to related innovation medicine research and development to the collaboration of bioactive molecule incorporation, be primarily present nanometer selenium size tune at present
Control, stability and bioactive molecule richness carry the problems such as efficiency.The present invention develops a kind of safely and effectively new oyster polypeptide-nanometer
Selenium is identified and is characterized its form, shows preferable antioxidant activity.A kind of new oyster polypeptide regulation preparation nanometer selenium
Method, and its application of the hepatic injury of antagonism alcohol and hydrogen peroxide-induced in vivo.
Another method that be designed to provide oyster polypeptide regulation preparation nanometer selenium of the invention.On the one hand more with oyster
Peptide is form stable nanometer selenium, on the other hand by the active factors incorporation nano granules of selenium in oyster polypeptide, realizes nanometer
The uniform, Stable distritation of grain, and there is oyster polypeptide and the active dual-functional nanometer granules of selenium of selenium simultaneously.The red simple substance prepared
Selenium has nano-grade size, and average grain diameter is the spheric granules less than 200 nm, and Se content is greater than 40%.
Another syllabus of the invention is the provision of oyster polypeptide regulation preparation nanometer selenium in antagonism alcohol or peroxidating
The application of hydrogen induced liver injury.
The purpose of the present invention is realized at least through one of following technical solution.
A kind of oyster polypeptide-nano granules of selenium compound preparation side with relieving alcoholism and protecting liver effect provided by the invention
Method includes the following steps:
(1) oyster decladding is taken into meat, homogenized is directly carried out after oyster meat is cleaned, obtain homogenate, water and work is added
Property enzyme, carry out enzyme digestion reaction, obtain enzymolysis liquid；
(2) by step (1) enzymolysis liquid, boiling water bath is handled, and supernatant is freeze-dried, obtains oyster by centrifuging and taking supernatant
Polypeptide powder (has the activation active oyster polypeptide of ADH)；
(3) step (2) oyster polypeptide (OPH) powder is added to the water, is uniformly mixed and is configured to oyster polypeptide solution, it will be sub-
Selenic acid sodium solution is added in oyster polypeptide solution, stirs evenly, obtains mixed liquor；
(4) while stirring, ascorbic acid solution is added drop-wise in step (3) described mixed liquor, is mixed when being added dropwise, water
Bath reaction, dialysis treatment obtain functionalized nano selenium sol；
(5) step (4) the functionalized nano selenium sol is ultrasonically treated, is freeze-dried (the pre-freezing temperature-of freeze-drying
30 DEG C -40 DEG C, material thickness 4-7 mm, drying time 24-35 h) after to obtain the oyster with relieving alcoholism and protecting liver effect more
Peptide-nano granules of selenium compound (OPH-SeNPs).
Further, the mass ratio of step (1) homogenate and water is 1:1-1:4；The homogenate rate is 10000-
15000 r/min；Homogenization time is 30-60 s.
Further, step (1) organized enzyme is papain, flavor protease, alkali protease, bromelain
One of enzyme, pancreatin, compound protease and neutral proteinase etc., the quality of the organized enzyme are oyster meat (substrate protein) matter
The 0.1-1wt% of amount；The temperature of the enzyme digestion reaction is 37-60 DEG C, and the time of enzyme digestion reaction is 0.5-12 h, enzyme digestion reaction
PH value is 5-11.
Preferably, the preferred pancreatin of the organized enzyme, enzymatic hydrolysis condition are as follows: pH 7.0-8.0, enzyme concentration contain for substrate protein
The 0.1-1 %(w/w of amount), reaction temperature is 40-60 DEG C, and the reaction time is 0.5-12 h.
Further, the time of step (2) the boiling water bath processing is 10-15min；The temperature of the centrifugation is taken the photograph for 4-10
Family name's degree, the revolving speed of centrifugation are 8000-10000 r/min, and the time of centrifugation is 10-30min.
Preferably, the vacuum degree of step (2) described freeze-drying is 1.0-1.5 mbar, and pre-freezing temperature is -30 DEG C -- 40
DEG C, material thickness is 4-7 mm, and drying time is 24-35 h.
Further, the oyster polypeptide (being abbreviated as OPH) has activation alcohol dehydrogenase (ADH) activity.
Further, the concentration of step (3) the oyster polypeptide solution is 0.5-10 mg/mL；The sodium selenite solution
Concentration be 0.5-2.0 mM；The volume ratio of the sodium selenite solution and oyster polypeptide solution is 1:1-4:1 (v/v).
Further, step (4) stirring rate while stirring is 100-500rpm, it is preferable that stirring
Rate is 100-200rpm；The concentration of the ascorbic acid solution is 1.0-8.0 mM；The mixed liquor and ascorbic acid solution
Volume ratio be 1:2-5:16 (v/v).
Further, the temperature of step (4) described water-bath is 25-45 DEG C, and the time of water-bath is 0.5-12
h；The bag filter specification that the dialysis treatment uses is 100-1000 Da, it is preferable that the specification of bag filter is 500-1000Da；
The time of the dialysis treatment is 36-48 h；The temperature of the dialysis treatment is 4-10 DEG C.
Further, the supersonic frequency of step (5) described ultrasonic treatment is 20-50 kHz, and the time of ultrasonic treatment is 5-
15 min, the temperature of ultrasonic treatment is less than 25 DEG C.
The present invention provides a kind of oyster polypeptide-nanometer selenium as made from above-mentioned preparation method with relieving alcoholism and protecting liver effect
Particle composites, abbreviation OPH-SeNPs.
Oyster polypeptide with relieving alcoholism and protecting liver effect-nano granules of selenium compound (OPH-SeNPs) provided by the invention is
Spheric granules, partial size is less than 200 nm, and wherein selenium element content is greater than 40 %(w/w).
The present invention provides the oyster polypeptide-nano granules of selenium compound (OPH-SeNPs) with relieving alcoholism and protecting liver effect,
It can be applied to prepare functional food or health care product.
Compared with prior art, the invention has the advantages that and the utility model has the advantages that
(1) it is multiple that oyster polypeptide-nano granules of selenium with relieving alcoholism and protecting liver effect can be not only made in preparation method provided by the invention
Close object, moreover it is possible to produce Oyster Protein source and promote the thick peptide of alcohol metabolism, production method is simple, and product Oyster Protein source promotees alcohol metabolism
Thick peptide obvious effect, has certain realistic meaning for large-scale production；
(2) preparation method provided by the invention regulates and controls preparation nanometer using the oyster polypeptide with activation ADH ability is template
Selenium, the oyster polypeptide-nano granules of selenium compound being prepared have good relieving alcoholism and protecting liver effect, on the one hand improve male
On the other hand the added value of oyster improves the stability of nanometer selenium again, the nanometer selenium prepared by oyster polypeptide regulation is in 4 DEG C of items
The time of stable preservation for one month, for Product transport, stores certain positive effect under part；
(3) oyster polypeptide-nano granules of selenium compound provided by the invention with relieving alcoholism and protecting liver effect, is detected through mtt assay, with
Single oyster polypeptide is compared, and is had stronger hepatoprotective activity, can be prevented alcohol and hydrogen peroxide from causing hepar damnification；Institute
State the one thousandth that oyster polypeptide-nano granules of selenium compound effective concentration is only oyster polypeptide (OPH).
Detailed description of the invention
Fig. 1 is compound for the oyster polypeptide-nano granules of selenium with relieving alcoholism and protecting liver effect prepared in case study on implementation 1 of the present invention
The transmission electron microscope picture of object；
Fig. 2 is the oyster polypeptide-nano granules of selenium compound with relieving alcoholism and protecting liver effect prepared in case study on implementation 2 of the present invention
Granularmetric analysis curve graph；
Fig. 3 be the oyster polypeptide-nano granules of selenium compound with relieving alcoholism and protecting liver effect prepared in case study on implementation 3 of the present invention and
The ADH activity ratio histogram of the oyster polypeptide of same protein concentration；
Fig. 4 be the oyster polypeptide-nano granules of selenium compound with relieving alcoholism and protecting liver effect prepared in case study on implementation 3 of the present invention and
Protective effect histogram of the oyster polypeptide of same protein concentration to Alcoholic to hepatic injury；
Fig. 5 is the oyster polypeptide-nano granules of selenium compound pair with relieving alcoholism and protecting liver effect prepared in case study on implementation 4 of the present invention
Hydrogen peroxide to hepatic injury protective effect histogram.
Specific embodiment
Specific implementation of the invention is described further below in conjunction with attached drawing and example, but implementation and protection of the invention
It is without being limited thereto.If it is existing to be that those skilled in the art can refer to it is noted that there is the not special process of detailed description below
Technology realize or understand.
In following embodiment, external ADH activity ratio using ADH kit, (build up, and article No. A083-2 includes: examination by Nanjing
Agent one, sodium pyrophosphate buffer solution；Reagent two, ethyl alcohol；Reagent three, NADH.) combination ADH enzyme solution measurement, the specific method is as follows: ADH
The dilution of one 0.65 mL(1:1 ultrapure water of kit buffer reagent), 0.75 mL reagent three is added, and (pulvis, 10 mL are super before use
Pure water dilution), 0.05 mL reagent two (ethyl alcohol) is added later, mixed liquor is used as after mixing；Experimental group adds 150 in 96 orifice plates
The polypeptide solution (blank group is with water replacement) of 0.5 mg/mL of μ L mixed liquor and 50 μ L, 37 DEG C of 5 min of incubation after mixing, then
The ADH enzyme solution of 20 μ L, 0.2 U/mL is added, since the timing sample blending is added measure A340nm, 10 s record single reading,
10 min of METHOD FOR CONTINUOUS DETERMINATION.Using the time as abscissa, A340nm is ordinate mapping, and slope is initial reaction rate, can be used as ADH
Opposite enzyme activity.ADH activity ratio calculation formula:
ADH activity ratio (%)=(V s -V 0 )/V 0 ×100%
Wherein,V 0 It is the initial reaction rate of negative control, andV S It is the initial reaction rate of test sample.
In following embodiment, the measuring method of partial size is as follows: by OPH- made from 1.2 mL different condition down regulations
SeNPs is added in measurement ware, using nano particle size instrument (Nano-ZS & MPT-2 type nano particle size instrument, Malvern company, Britain)
Its size distribution, granule absorbance 0.001 are measured, dispersing agent is water, and the refractive index of dispersing agent is 1.330, and test temperature is
25 DEG C, obtain average particle size value (nm) and grain size volume distribution map.
Embodiment 1
A kind of oyster polypeptide-nano granules of selenium compound preparation method with relieving alcoholism and protecting liver effect, includes the following steps:
(1) using commercially available oyster as raw material, directly (homogenate speed is 12000 r/ for homogenate after its decladding is taken meat, cleaned meat
Min, Homogenization time s), form homogenate for 60, add water (mass ratio of homogenate and water is 1:1), pancreatin is added
Pancreatin is digested, enzymatic hydrolysis condition are as follows: pH 7.0, enzyme additive amount are 0.1%(w/w) (i.e. enzyme additive amount is oyster meat
The 0.1% of amount), 60 DEG C of reaction temperature, 4 h of reaction time.After enzyme digestion reaction, 10 min of boiling water bath enzyme deactivation, at 4 DEG C
8000 r/min, 30 min of centrifugation, the freeze-drying of gained supernatant (- 40 DEG C of the pre-freezing temperature of freeze-drying, 7 mm of material thickness,
Drying time 35 h), obtains oyster polypeptide powder (OPH)；
(2) step (1) the oyster polypeptide powder is added to the water, is uniformly mixed and is configured to oyster polypeptide solution (concentration 10
Mg/mL), the sodium selenite solution that concentration is 2 mM is added to the oyster polypeptide solution that concentration is 10 mg/mL, stirred evenly
Afterwards, the ascorbic acid solution that concentration is 8.0 mM is added dropwise, wherein oyster polypeptide solution, sodium selenite solution, ascorbic acid solution
Ratio is 1:4:4(v/v/v), (500 rpm) is mixed when being added dropwise, after 0.5 h is reacted under 25 DEG C of water-baths, with 100 Da's
Bag filter obtains functionalized nano selenium sol in 4 DEG C of 48 h of dialysis；Functionalized nano selenium sol colloidal sol is defeated by ultrasonic wave
20 kHz of frequency out, ultrasonic time be 5 min ultrasonication after (in ultrasonic procedure with ice bath control sample temperature be lower than 25
DEG C), (- 40 DEG C of pre-freezing temperature, 7 mm of material thickness, drying time 35 h) obtains described with relieving alcoholism and protecting liver after freeze-drying
Oyster polypeptide-nano granules of selenium compound of effect.Its pattern is observed by transmission electron microscope (TEM), it as a result as shown in Figure 1, should
Compound is spherical particles, and average grain diameter is less than 200 nm.The effect of other embodiments is similar to Example 1, can refer to Fig. 1
It is shown.
Embodiment 2
A kind of oyster polypeptide-nano granules of selenium compound preparation method with relieving alcoholism and protecting liver effect, includes the following steps:
(1) using commercially available oyster as raw material, directly (homogenate speed is 12000 r/ for homogenate after its decladding is taken meat, cleaned meat
Min, Homogenization time are 60 s), to form homogenate, add water (mass ratio of homogenate and water is 1:1), be added pancreatin Papain into
Row enzymatic hydrolysis, enzymatic hydrolysis condition are as follows: pH 5, enzyme additive amount be 1 %(w/w) (i.e. enzyme additive amount be oyster meat quality 1%), react warm
37 DEG C of degree, 12 h of reaction time.After enzyme digestion reaction, 15 min of boiling water bath enzyme deactivation, 10000 r/min are centrifuged at 10 DEG C
10 min, gained supernatant are freeze-dried (- 30 DEG C of the pre-freezing temperature of freeze-drying, 4 mm of material thickness, drying time 24
H), oyster polypeptide powder (OPH) is obtained；
(2) step (1) the oyster polypeptide powder is added to the water, is uniformly mixed and is configured to oyster polypeptide solution (concentration is
5mg/mL)；The sodium selenite solution that concentration is 0.5 mM is added to the oyster polypeptide solution that concentration is 5 mg/mL, stirring is equal
The ascorbic acid solution that concentration is 2 mM is added dropwise after even, wherein oyster polypeptide solution, sodium selenite solution, ascorbic acid solution
Ratio is 1:1:4(v/v/v), (100 rpm) is mixed when being added dropwise, it is saturating with 1000 Da after 12 h are reacted under 45 DEG C of water-baths
Bag is analysed in 10 DEG C of 36 h of dialysis, obtains functionalized nano selenium sol；Functionalized nano selenium sol colloidal sol is exported by ultrasonic wave
50 kHz of frequency, ultrasonic time be 15 min ultrasonication after (in ultrasonic procedure with ice bath control sample temperature be lower than 25
DEG C), (- 30 DEG C of pre-freezing temperature, 4 mm of material thickness, drying time 24 h) obtains described with relieving alcoholism and protecting liver effect after freeze-drying
Oyster polypeptide-nano granules of selenium compound of fruit.Measure its particle diameter distribution with Zeta nano particle size instrument, as a result as shown in Fig. 2, its
Average grain diameter is less than 200 nm.The effect of other embodiments is similar to Example 2, can refer to shown in Fig. 2.
Embodiment 3
A kind of oyster polypeptide-nano granules of selenium compound preparation method with relieving alcoholism and protecting liver effect, includes the following steps:
(1) using commercially available oyster as raw material, directly (homogenate speed is 12000 r/ for homogenate after its decladding is taken meat, cleaned meat
Min, Homogenization time s), form homogenate for 60, add water (mass ratio of homogenate and water is 1:2), alkali protease is added
Alcalase2.4 L is digested, enzymatic hydrolysis condition are as follows: pH 11, enzyme additive amount are 0.4%(w/w) (i.e. enzyme additive amount is oyster meat
The 0.4% of quality), 40 DEG C of reaction temperature, 0.5 h of reaction time.After enzyme digestion reaction, 10 min of boiling water bath enzyme deactivation, at 4 DEG C
Lower 10000 r/min are centrifuged 15 min, and gained supernatant is freeze-dried (- 35 DEG C of the pre-freezing temperature of freeze-drying, material thickness 5
Mm, drying time 30 h), obtain oyster polypeptide powder (OPH)；
(2) step (1) the oyster polypeptide powder is added to the water, is uniformly mixed and is configured to oyster polypeptide solution (concentration 0.5
It mg/mL), is oyster polypeptide that 1.5 mM sodium selenite solutions are added to that concentration is 0.5 mg/mL by concentration, after mixing evenly
Dropwise addition concentration is 6 mM ascorbic acid solutions, and wherein oyster polypeptide solution, sodium selenite solution, ascorbic acid solution ratio are
1:1:6.4(v/v/v), (150 rpm) is mixed when being added dropwise, after 6 h are reacted under 35 DEG C of water-baths, with the bag filter of 500 Da
In 4 DEG C of 36 h of dialysis, functionalized nano selenium sol is obtained；Functionalized nano selenium sol colloidal sol is passed through into ultrasonic output frequency
20 kHz, ultrasonic time be 10 min ultrasonication after (in ultrasonic procedure with ice bath control sample temperature be lower than 25 DEG C),
After freeze-drying (- 35 DEG C of pre-freezing temperature, 5 mm of material thickness, drying time 24 h) obtain after obtain described there is relieving alcoholism and protecting liver
Oyster polypeptide-nano granules of selenium compound of effect.
Under same protein concentration, oyster polypeptide-nano granules of selenium compound (OPH- with relieving alcoholism and protecting liver effect
SeNPs) compared with oyster peptide (OPH), OPH-SeNPs is apparently higher than oyster peptide to the activity ratio of ADH, and is in agent with protein concentration
Magnitude relation, as a result as shown in Figure 3.Oyster peptide and oyster polypeptide-nano granules of selenium compound are detected to alcoholic liver injury through MTT
Protective effect, as a result as shown in Figure 4.Other embodiments effect is similar to Example 3, can refer to Fig. 3 and Fig. 4.
Embodiment 4
A kind of oyster polypeptide-nano granules of selenium compound preparation method with relieving alcoholism and protecting liver effect, includes the following steps:
(1) using commercially available oyster as raw material, directly (homogenate speed is 12000 r/ for homogenate after its decladding is taken meat, cleaned meat
Min, Homogenization time s), form homogenate for 60, add water (mass ratio of homogenate and water is 1:4), pancreatin is added
Pancreatin is digested, enzymatic hydrolysis condition are as follows: pH 7.0, enzyme additive amount are 1%(w/w) (indicate that enzyme additive amount is oyster meat
The 1% of amount), 55 DEG C of reaction temperature, 2 h of reaction time.After enzyme digestion reaction, 15 min of boiling water bath enzyme deactivation, at 10 DEG C
10000 r/min are centrifuged 30 min, and gained supernatant is freeze-dried (- 35 DEG C of the pre-freezing temperature of freeze-drying, material thickness 5
Mm, drying time 30 h), obtain oyster polypeptide powder (OPH)；
(2) step (1) the oyster polypeptide powder is added to the water, is uniformly mixed and is configured to oyster polypeptide solution (concentration 5
Mg/mL), it is oyster polypeptide that 1.5 mM sodium selenite solutions are added to that concentration is 5 mg/mL by concentration, drips after mixing evenly
Adding concentration is 6 mM ascorbic acid solutions, and wherein oyster polypeptide solution, sodium selenite solution, ascorbic acid solution ratio are 1:
4:4(v/v/v), (150 rpm) is mixed when being added dropwise, after 6 h are reacted under 25 DEG C of water-baths, with the bag filter of 500 Da at 4 DEG C
Dialyse 36 h, obtains functionalized nano selenium sol；Functionalized nano selenium sol colloidal sol is passed through into 50 kHz of ultrasonic output frequency,
(sample temperature being controlled with ice bath in ultrasonic procedure and is lower than 25 DEG C), freezing is dry after the ultrasonication that ultrasonic time is 10 min
(- 35 DEG C of pre-freezing temperature, 5 mm of material thickness, drying time 30 h), obtains described male with relieving alcoholism and protecting liver effect after dry
Oyster polypeptide-nano granules of selenium compound.
Have oyster polypeptide-nano granules of selenium compound (OPH-SeNPs) of relieving alcoholism and protecting liver effect to mistake through mtt assay detection
The protective effect of the hepatic injury of hydrogen oxide induction, as a result as shown in Figure 5.Other embodiments effect is similar to Example 4, can refer to
Shown in Fig. 5.
For above-described embodiment it is found that being digested with pancreatin Pancreatin, the oyster peptide that 4 h are obtained is template, and
Under its 10 mg/mL concentration, after mixing evenly with 2 mM sodium selenite solutions, when being added dropwise one section of the reaction of 8 mM ascorbic acid
Between after (wherein oyster polypeptide, sodium selenite, ascorbic acid volume ratio be 1:4:4), through 5 min of ultrasound, obtain described in have
Oyster polypeptide-nano granules of selenium compound of relieving alcoholism and protecting liver effect, for close to spherical particles, average grain diameter is less than 200 nm
(Fig. 1).It is digested with Papain, the oyster peptide that 12 h are obtained is template, and under its 5 mg/mL concentration, with 0.5 mM
Sodium selenite solution after mixing evenly, be added dropwise 2 mM ascorbic acid reaction a period of time after (wherein oyster polypeptide, selenous acid
Sodium, ascorbic acid volume ratio are 1:1:4), through 15 min of ultrasound, obtain the oyster polypeptide-with relieving alcoholism and protecting liver effect
Nano granules of selenium compound, average grain diameter is less than 200 nm(Fig. 2).It is digested with Alcalase2.4 L, 0.5 h is obtained
Oyster peptide be template, and under its 0.5 mg/mL concentration, after mixing evenly with 1.5 mM sodium selenite solutions, be added dropwise
6 mM ascorbic acid react after a period of time (wherein oyster polypeptide, sodium selenite, ascorbic acid volume ratio are 1:1:6.4),
Through 10 min of ultrasound, the oyster polypeptide-nano granules of selenium compound with relieving alcoholism and protecting liver effect is obtained, it is dense in same protein
Lower its ADH activity ratio activity compared with oyster peptide more preferable (Fig. 3) is spent, detects oyster polypeptide-nano granules of selenium compound through mtt assay
Protective effect to alcohol-induced hepatic injury, is as a result shown in Fig. 4.It is digested with pancreatin Pancreatin, the oyster that 2 h are obtained
Peptide is template, and under its 5 mg/mL concentration, after mixing evenly with 1.5 mM sodium selenite solutions, anti-6 mM are added dropwise
Bad hematic acid reacts after a period of time (wherein oyster polypeptide, sodium selenite, ascorbic acid volume ratio are 1:4:4), through ultrasound 10
Min obtains the oyster polypeptide-nano granules of selenium compound with relieving alcoholism and protecting liver effect and detects it to hydrogen peroxide through MTT
The protective effect of the hepatic injury of induction, is as a result shown in Fig. 5.
Above embodiments are only preferrred embodiment of the present invention, for explaining only the invention, are not intended to limit the present invention, this
Field technical staff should belong to guarantor of the invention without departing from change made under spirit of the invention, replacement, modification etc.
Protect range.
Claims (10)
1. a kind of oyster polypeptide-nano granules of selenium compound preparation method with relieving alcoholism and protecting liver effect, which is characterized in that packet
Include following steps:
(1) oyster decladding is taken into meat, carries out homogenized after oyster meat is cleaned, obtain homogenate, water and organized enzyme is added,
Enzyme digestion reaction is carried out, enzymolysis liquid is obtained；
(2) by step (1) enzymolysis liquid, boiling water bath is handled, and supernatant is freeze-dried, obtains oyster by centrifuging and taking supernatant
Polypeptide powder；
(3) step (2) the oyster polypeptide powder is added to the water, is uniformly mixed and is configured to oyster polypeptide solution, by selenous acid
Sodium solution is added in oyster polypeptide solution, stirs evenly, obtains mixed liquor；
(4) while stirring, ascorbic acid solution is added drop-wise in step (3) described mixed liquor, is uniformly mixed, water-bath is anti-
It answers, dialysis treatment, obtains functionalized nano selenium sol；
(5) step (4) the functionalized nano selenium sol is ultrasonically treated, is obtained after freeze-drying described with relieving alcoholism and protecting liver effect
Oyster polypeptide-nano granules of selenium compound of fruit.
2. preparation method according to claim 1, which is characterized in that step (1) the homogenate rate is 10000-15000
R/min, Homogenization time are 30-60 s；The mass ratio of the homogenate and water is 1:1-1:4.
3. preparation method according to claim 1, which is characterized in that step (1) organized enzyme is papain, wind
One of taste protease, alkali protease, bromelain, pancreatin, compound protease and neutral proteinase, the organized enzyme
Quality be oyster meat quality 0.1-1wt%；The temperature of the enzyme digestion reaction is 37-60 DEG C, and the time of enzyme digestion reaction is
0.5-12 h, the pH value of enzyme digestion reaction are 5-11.
4. preparation method according to claim 1, which is characterized in that the time of step (2) the boiling water bath processing is 10-
15min；The temperature of the centrifugation is 4-10 degrees Celsius, and the revolving speed of centrifugation is 8000-10000 r/min, and the time of centrifugation is 10-
30min。
5. preparation method according to claim 1, which is characterized in that the concentration of step (3) the oyster polypeptide solution is
0.5-10 mg/mL；The concentration of the sodium selenite solution is 0.5-2.0 mM；The sodium selenite solution and oyster polypeptide are molten
The volume ratio of liquid is 1:1-4:1.
6. preparation method according to claim 1, which is characterized in that step (4) stirring while stirring
Rate is 100-500rpm；The concentration of the ascorbic acid solution is 1.0-8.0 mM；The mixed liquor and ascorbic acid solution
Volume ratio be 1:2-5:16.
7. preparation method according to claim 1, which is characterized in that the temperature of step (4) described water-bath is 25-45
DEG C, the time of water-bath is 0.5-12 h；The bag filter specification that the dialysis treatment uses is 100-1000 Da；It is described
The time of analysis processing is 36-48 h；The temperature of the dialysis treatment is 4-10 DEG C.
8. preparation method according to claim 1, which is characterized in that the supersonic frequency of step (5) described ultrasonic treatment is
The time of 20-50 kHz, ultrasonic treatment are 5-15 min, and the temperature of ultrasonic treatment is less than 25 DEG C.
9. a kind of oyster polypeptide-as made from the described in any item preparation methods of claim 1-8 with relieving alcoholism and protecting liver effect
Nano granules of selenium compound.
10. oyster polypeptide-nano granules of selenium compound described in claim 9 with relieving alcoholism and protecting liver effect is in the functional food of preparation
Application in product or health care product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910581455.XA CN110200287B (en) | 2019-06-29 | 2019-06-29 | Oyster polypeptide-nano selenium particle compound with effects of dispelling effects of alcohol and protecting liver as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910581455.XA CN110200287B (en) | 2019-06-29 | 2019-06-29 | Oyster polypeptide-nano selenium particle compound with effects of dispelling effects of alcohol and protecting liver as well as preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110200287A true CN110200287A (en) | 2019-09-06 |
| CN110200287B CN110200287B (en) | 2023-06-09 |
Family
ID=67795476
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910581455.XA Active CN110200287B (en) | 2019-06-29 | 2019-06-29 | Oyster polypeptide-nano selenium particle compound with effects of dispelling effects of alcohol and protecting liver as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110200287B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112710822A (en) * | 2020-12-17 | 2021-04-27 | 四川农业大学 | In-vitro simulated digestion method for edible fungus polysaccharide and selenizing derivative thereof |
| CN113142589A (en) * | 2021-02-25 | 2021-07-23 | 广东维尔嘉康生命科技有限公司 | Liver-protecting hexapeptide composition and preparation method thereof |
| CN113875989A (en) * | 2021-09-23 | 2022-01-04 | 山东安为先生物科技有限公司 | Functionalized water-soluble nano-selenium, preparation method and application |
| CN114836505A (en) * | 2022-05-19 | 2022-08-02 | 泸州品创科技有限公司 | Micromolecular hangover-alleviating peptide and preparation method and application thereof |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762380A (en) * | 2005-09-30 | 2006-04-26 | 烟台益生药业有限公司 | Nano selenium product and its preparation method |
| CN1947792A (en) * | 2005-10-13 | 2007-04-18 | 暨南大学 | Method for preparing active protein composition type nano-selenium and its liquid phase storage tech. |
| CN101972004A (en) * | 2010-09-01 | 2011-02-16 | 江苏大学 | Preparation method and application of oyster zymolyte |
| CN102763849A (en) * | 2012-08-08 | 2012-11-07 | 张颜青 | Health care product containing nano-selenium |
| US20130029931A1 (en) * | 2011-07-25 | 2013-01-31 | The Hong Kong Polytechnic University | Pleurotus tuber-regium polysaccharide functionalized nano-selenium hydrosol with anti-tumor activity and preparation method thereof |
| CN103271353A (en) * | 2013-05-08 | 2013-09-04 | 天津市星河系科技有限公司 | Liver protecting and health care composition and preparation method thereof |
| CN104174008A (en) * | 2014-07-30 | 2014-12-03 | 华南理工大学 | Method for preparing walnut polypeptide nano-selenium with anti-tumor activity |
| CN105200105A (en) * | 2015-09-10 | 2015-12-30 | 烟台源力德海洋生物有限公司 | Preparation method of oyster protein liver protecting peptide preparation |
| CN105296584A (en) * | 2015-10-09 | 2016-02-03 | 烟台源力德海洋生物有限公司 | Preparation method of marine biological health care peptide preparation |
| CN105918755A (en) * | 2016-05-16 | 2016-09-07 | 青岛海和创兴海洋生物科技有限公司 | Nano-selenium oyster polysaccharide solid drink |
| CN106243187A (en) * | 2016-09-05 | 2016-12-21 | 华南理工大学 | A kind of method of sharp separation oyster peptide from Concha Ostreae enzymolysis solution |
| CN107343658A (en) * | 2017-06-13 | 2017-11-14 | 程榆茗 | A kind of antifatigue SOD oyster peptides complex capsule and preparation method thereof |
| US20170362629A1 (en) * | 2015-11-05 | 2017-12-21 | Infinitus (China) Company Ltd | Oyster peptide capable of enhancing sexual function, and preparation method and application thereof |
| CN107581619A (en) * | 2017-09-11 | 2018-01-16 | 陈石良 | A kind of antifatigue anti senility sea cucumber oyster peptide complex capsule and preparation method thereof |
-
2019
- 2019-06-29 CN CN201910581455.XA patent/CN110200287B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762380A (en) * | 2005-09-30 | 2006-04-26 | 烟台益生药业有限公司 | Nano selenium product and its preparation method |
| CN1947792A (en) * | 2005-10-13 | 2007-04-18 | 暨南大学 | Method for preparing active protein composition type nano-selenium and its liquid phase storage tech. |
| CN101972004A (en) * | 2010-09-01 | 2011-02-16 | 江苏大学 | Preparation method and application of oyster zymolyte |
| US20130029931A1 (en) * | 2011-07-25 | 2013-01-31 | The Hong Kong Polytechnic University | Pleurotus tuber-regium polysaccharide functionalized nano-selenium hydrosol with anti-tumor activity and preparation method thereof |
| CN102763849A (en) * | 2012-08-08 | 2012-11-07 | 张颜青 | Health care product containing nano-selenium |
| CN103271353A (en) * | 2013-05-08 | 2013-09-04 | 天津市星河系科技有限公司 | Liver protecting and health care composition and preparation method thereof |
| CN104174008A (en) * | 2014-07-30 | 2014-12-03 | 华南理工大学 | Method for preparing walnut polypeptide nano-selenium with anti-tumor activity |
| CN105200105A (en) * | 2015-09-10 | 2015-12-30 | 烟台源力德海洋生物有限公司 | Preparation method of oyster protein liver protecting peptide preparation |
| CN105296584A (en) * | 2015-10-09 | 2016-02-03 | 烟台源力德海洋生物有限公司 | Preparation method of marine biological health care peptide preparation |
| US20170362629A1 (en) * | 2015-11-05 | 2017-12-21 | Infinitus (China) Company Ltd | Oyster peptide capable of enhancing sexual function, and preparation method and application thereof |
| CN105918755A (en) * | 2016-05-16 | 2016-09-07 | 青岛海和创兴海洋生物科技有限公司 | Nano-selenium oyster polysaccharide solid drink |
| CN106243187A (en) * | 2016-09-05 | 2016-12-21 | 华南理工大学 | A kind of method of sharp separation oyster peptide from Concha Ostreae enzymolysis solution |
| CN107343658A (en) * | 2017-06-13 | 2017-11-14 | 程榆茗 | A kind of antifatigue SOD oyster peptides complex capsule and preparation method thereof |
| CN107581619A (en) * | 2017-09-11 | 2018-01-16 | 陈石良 | A kind of antifatigue anti senility sea cucumber oyster peptide complex capsule and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112710822A (en) * | 2020-12-17 | 2021-04-27 | 四川农业大学 | In-vitro simulated digestion method for edible fungus polysaccharide and selenizing derivative thereof |
| CN113142589A (en) * | 2021-02-25 | 2021-07-23 | 广东维尔嘉康生命科技有限公司 | Liver-protecting hexapeptide composition and preparation method thereof |
| CN113875989A (en) * | 2021-09-23 | 2022-01-04 | 山东安为先生物科技有限公司 | Functionalized water-soluble nano-selenium, preparation method and application |
| CN113875989B (en) * | 2021-09-23 | 2023-09-29 | 山东安为先生物科技有限公司 | Functionalized water-soluble nano-selenium, preparation method and application |
| CN114836505A (en) * | 2022-05-19 | 2022-08-02 | 泸州品创科技有限公司 | Micromolecular hangover-alleviating peptide and preparation method and application thereof |
| CN114836505B (en) * | 2022-05-19 | 2024-04-12 | 泸州品创科技有限公司 | Small molecule anti-alcohol peptide and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110200287B (en) | 2023-06-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110200287A (en) | A kind of oyster polypeptide-nano granules of selenium compound and the preparation method and application thereof with relieving alcoholism and protecting liver effect | |
| CN107177567B (en) | A kind of liquid enzyme formulation and preparation method | |
| CN106993813B (en) | Preparation method of probiotic microcapsules | |
| KR20210088408A (en) | Lactobacillus Plantarum and uses thereof | |
| WO2022068615A1 (en) | Formulation for improving activity and/or thermostability of superoxide dismutases and use thereof | |
| Santacruz et al. | Viability of free and encapsulated Lactobacillus acidophilus incorporated to cassava starch edible films and its application to Manaba fresh white cheese | |
| CN102994395A (en) | Aureobasidium pullulans and application thereof | |
| CN108424942A (en) | A kind of carrier material of glucityl core-shell structure and its preparation and application | |
| CN110777097A (en) | Lactobacillus strain with strong acid resistance and screening and fermenting processes thereof | |
| CN109007807A (en) | A kind of microcapsules fruit ferment powder and preparation method thereof | |
| CN108324731A (en) | It improves antioxidant activity and enhances the preparation method and applications of the biological polyoses particle of bacteriostasis | |
| CN108048349B (en) | Preparation method and application of lactobacillus paracasei N1115 embedded bacteria powder | |
| CN106616171A (en) | Probiotic beverage containing saussurea involucrate culture and preparation method thereof | |
| CN114916675A (en) | Water-in-oil-in-water type multiple emulsion gel bead for improving survival rate of probiotics, preparation method and application | |
| CN108324588A (en) | Mouthwash and preparation method thereof containing lysozyme | |
| CN109527099A (en) | A kind of fructus lycii compound probiotic goat milk piece and preparation method thereof | |
| CN116286371B (en) | Freeze-drying protective agent formula for improving freeze-drying effect of strain | |
| TWI595088B (en) | A medium for mycelia of antrodia cinnamomea | |
| CN116515808A (en) | Probiotic microcapsule and application thereof | |
| CN107646531A (en) | A kind of preparation method of Se-rich lucid ganoderma culture medium | |
| CN116570029A (en) | Walnut oligopeptide probiotics microcapsule and preparation method thereof | |
| CN114747769A (en) | Probiotic product and preparation method thereof | |
| CN107896921A (en) | The white fungus bacterium bioconversion composition and its manufacture method of maca and RHIIZOMA DIOSCOREAE from Henan of China | |
| CN114190558A (en) | Oral instant film containing probiotics and preparation method thereof | |
| TW202019395A (en) | Lactic acid bacteria crystal composition capable of promoting intestinal stem cell proliferation, antiviral, anti-inflammatory and anti-allergic effects and preparation method thereof. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |