CN110199779B

CN110199779B - Preparation method of mushroom black fungus plastic mold strain

Info

Publication number: CN110199779B
Application number: CN201910521395.2A
Authority: CN
Inventors: 应国华; 吕明亮; 李伶俐; 薛振文
Original assignee: LISHUI ACADEMY OF FORESTRY
Current assignee: LISHUI ACADEMY OF FORESTRY
Priority date: 2019-06-17
Filing date: 2019-06-17
Publication date: 2021-11-05
Anticipated expiration: 2039-06-17
Also published as: CN110199779A

Abstract

The invention discloses a method for preparing mushroom black fungus plastic mold strains, which comprises the following steps: manufacturing a strain mould which is of a nearly conical structure and comprises a circular bottom edge and a cylindrical head, wherein 3-9 arc-shaped flat long strip-shaped ribs are arranged on the bottom edge and are collected on the cylindrical head according to a certain radian, so that a nearly conical hollow mould is formed; proportionally mixing the coarse particle sawdust, the fine particle sawdust, the wheat bran, the corn flour and the gypsum powder with water, soaking and boiling the mixture with the water until no white core exists, uniformly mixing the mixture with the wheat grains, adding a quantitative strain mold, stirring the mixture uniformly, and picking out the mold filled with the culture material; according to the number of the plastic molds, adding culture materials as fillers between the plastic molds, bagging, sterilizing, cooling, inoculating and culturing. The strain obtained by the invention has the advantages of quick and uniform strain breaking, no need of punching, high inoculation efficiency, low pollution rate and the like.

Description

Preparation method of mushroom black fungus plastic mold strain

Technical Field

The invention relates to a production technology of edible fungus strains, in particular to a preparation method of mushroom black fungus plastic mold strains.

Background

The mushroom and the black fungus are two edible fungus industries with the largest output in China, and the annual output of the fresh mushroom and the black fungus reaches 1500 ten thousand tons. The production mode of the mushroom black fungus in the southern main production area has a fungus stick type. The strains used at present are mainly wood chip strains, and need punch, break off with fingers and divide the strain, fill in the kind of a seed during inoculation, the wood chip strain often breaks off with fingers and divides the strain speed slower, the size is not too even, has influenced inoculation efficiency and effect, is unfavorable for intensification, large-scale production, and black fungus all adopts wood chip strain, and the finger extrusion during inoculation, damages the strain easily, influences the survival rate. The other strain is a capsule strain with consistent specification, overcomes the defects that the traditional wood chip strain is slow and uneven in strain breaking and separating and the inoculation speed is improved compared with that of the wood chip strain, but the defects are obvious, and firstly, the capsule has small strain quantity, is not durable to store and has short storage life; secondly, specialized equipment is needed for producing the capsule strains, investment is large, cost is high, the capsule strains are not suitable for self production of general bases and households, the cost for purchasing the capsule strains is higher than 0.1 yuan per rod, thirdly, secondary infection hidden dangers exist when the strains are cultured, crushed and then compressed into capsules, and the capsule strains are only limited to be popularized in a small amount in partial areas at present. Over ten years, 3 types of strain molds have been seen, and collection tests show that the strain molds have several defects, so that the strain molds are not applied to production until now. Firstly, the sharp head part of the plastic mould is quite sharp, the hand is easily injured when the plastic mould is bottled and bagged, and the bag is easily punctured when seeds are produced; secondly, the dimension of the mould structure is unreasonable, especially the mould area of the upright post and the tip is large, which affects the contact area between the strain inoculated into the material stick and the material stick, the spawn running is slow, and the inoculation survival rate is affected. Thirdly, the unreasonable structure and size of the mould leads to higher cost of the mould and the applied mould strains, and has no competitive advantage.

Disclosure of Invention

In order to solve the problems, the invention provides a preparation method of a mushroom black fungus plastic mold strain.

In order to achieve the purpose, the invention adopts the technical scheme that:

a preparation method of mushroom black fungus plastic mold strains comprises the following steps:

s1 mould for making strain

The strain mold is of a nearly conical structure and comprises a circular bottom edge and a cylindrical head, wherein 3-9 arc flat long strip-shaped ribs are uniformly arranged on the bottom edge and are converged at the cylindrical head according to a certain radian to form a nearly conical hollow mold, and the strain mold is obtained by manufacturing a grinding tool according to the size and performing injection molding;

s2, weighing the following components in percentage by mass: 25-50% of coarse particle sawdust, 25-50% of fine particle sawdust, 10-20% of wheat bran, 5-15% of corn flour, 10-30% of wheat grains and 1-2% of gypsum powder;

s3, adding water into the weighed coarse particle sawdust, fine particle sawdust, wheat bran, corn flour and gypsum powder, stirring uniformly, soaking and boiling the mixture with the water until the wheat grains are free of white centers, stirring uniformly until the water content of the compost reaches 50-60%, adding a strain mold with the dry weight of 10-40% of the raw materials, stirring uniformly, filling the inner cavity of the mold with the compost, and picking out the mold filled with the compost;

s4, according to the number of the plastic molds, adding culture materials as fillers between the plastic molds after bagging, wherein the adding amount is based on the standard that culture materials are filled between the adjacent plastic molds, after the culture materials are added, plastic bagging is carried out, after the bagging, a ferrule is provided with a cotton cover body, whether bags are broken or not is checked, an adhesive tape is pasted at the broken bag position, after the bagging, an outer sleeve bag is sleeved, and the inoculation culture is carried out after sterilization and cooling.

Furthermore, under the condition of an inoculation box or open inoculation, the mould strains are directly pressed into the material rod by hands or knocked into the material rod by a hammer to finish inoculation without punching the material rod.

Further, the overall length of the nearly conical structure is set to be 2.5-5 cm, wherein: the inner diameter of the bottom edge of the circular ring of the main body is 1-3 cm, the width is 0.1-0.5 cm, and the wall thickness is 0.1-0.5 cm; the width of the arc-shaped flat and long strip-shaped rib column is 0.1-0.5 cm, the thickness of the arc-shaped flat and long strip-shaped rib column is 0.1-0.4 cm, the length of the cylindrical head is 0.3-1 cm, the diameter of the cylindrical head is 0.1-0.5 cm, and the top of the cylindrical head is arc-shaped.

The invention has the following beneficial effects:

the mould strain is suitable for stick inoculation of wood-rotting edible fungi such as mushrooms, black fungus and the like, and has the advantages of quick and uniform strain breaking, no need of punching, high inoculation efficiency, low pollution rate, good economy and the like.

Drawings

FIG. 1 is a schematic structural diagram of a seed mold in an embodiment of the invention.

Detailed Description

The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.

Examples

s1 mould for making strain

As shown in fig. 1, the strain mold is of a nearly conical structure and comprises a circular bottom edge and a cylindrical head, 3-9 arc flat long strip-shaped ribs are uniformly arranged on the bottom edge and are gathered at the cylindrical head according to a certain radian to form a nearly conical hollow mold, and the strain mold is obtained by manufacturing a grinding tool according to the size and performing injection molding; the total length of the mold of the nearly conical structure is set to be 2.5-5 cm, wherein: the inner diameter of the bottom edge of the circular ring of the main body is 1-3 cm, the width is 0.1-0.5 cm, and the wall thickness is 0.1-0.5 cm; the width of the arc-shaped flat long strip-shaped rib column is 0.1-0.5 cm, the thickness of the rib column is 0.1-0.4 cm, the length of the cylindrical head is 0.3-1 cm, the diameter of the cylindrical head is 0.1-0.5 cm, and the top of the cylindrical head is arc-shaped;

s4, according to the number of the plastic molds, adding culture materials as fillers between the plastic molds after bagging, wherein the adding amount is based on the standard that culture materials are filled between adjacent plastic molds, the number of the plastic molds in each bag is increased as much as possible to reach the number of the multiple culture material rods of each strain, the economy of the plastic molds is improved, after the culture materials are added, the plastic molds are bagged by adopting a manual bagging method or a punching bagging machine, after the bags are bagged, a ferrule is provided with no cotton cover body, whether the bags are broken or not is checked, after the bags are broken, the ferrule is sleeved with an outer bag, sterilization, cooling, inoculation and culture are carried out, the temperature of a culture room is controlled to be 20-27 ℃, the humidity is 50-70%, ventilation is carried out for 1-2 times every day, and shading culture is carried out. The two-section type bagging can solve the problems that when the plastic molds are mixed with the culture materials, the cavity is not filled with a part of the plastic molds with too few raw materials, and the plastic molds are not detected and are proportionally filled again when too many culture materials are mixed, so that the number of the plastic molds in one strain is small, and the space is wasted.

The strain can be inoculated by directly pressing the mould strain into the material rod by hand or knocking the mould strain into the material rod by a hammer without punching the material rod under the conditions of an inoculation box or open inoculation. The strain and the inoculation method have the characteristics of convenience, rapidness, high inoculation speed, early strain feed recovery and high survival rate.

The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.

Claims

1. A preparation method of mushroom black fungus plastic mold strains is characterized by comprising the following steps: the method comprises the following steps:

s1 mould for making strain

The strain mould is of a nearly conical structure and comprises a circular bottom edge and a cylindrical head, wherein 3-9 arc flat long strip-shaped ribs are uniformly arranged on the bottom edge and are collected on the cylindrical head according to a certain radian, so that a nearly conical hollow mould is formed; the overall length of nearly circular cone column structure sets up to 2.5 ~ 5cm, wherein: the inner diameter of the bottom edge of the circular ring of the main body is 1-3 cm, the width is 0.1-0.5 cm, and the wall thickness is 0.1-0.5 cm; the width of the arc-shaped flat long strip-shaped rib column is 0.1-0.5 cm, the thickness of the rib column is 0.1-0.4 cm, the length of the cylindrical head is 0.3-1 cm, the diameter of the cylindrical head is 0.1-0.5 cm, and the top of the cylindrical head is arc-shaped; the material is obtained by manufacturing a mould according to the size and performing injection molding;

s4, according to the number of the plastic molds, adding culture materials as fillers between the plastic molds after bagging, wherein the adding amount is based on the standard that culture materials are filled between the adjacent plastic molds, after the culture materials are added, plastic bagging is carried out, after bagging, a ferrule has no cotton cover body, whether bags are broken or not is checked, an adhesive tape is pasted at the broken bag position, after the bagging, an outer sleeve bag is sleeved, and sterilization, cooling and inoculation culture are carried out;

under the condition of an inoculation box or open inoculation, the mould strains are directly pressed into the material rod by hands or knocked into the material rod by a hammer without punching the material rod first, so that inoculation is completed.