CN110195031A - A kind of antidote preparation method degraded vomitoxin and protect intestinal mucosa - Google Patents
A kind of antidote preparation method degraded vomitoxin and protect intestinal mucosa Download PDFInfo
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- CN110195031A CN110195031A CN201910416054.9A CN201910416054A CN110195031A CN 110195031 A CN110195031 A CN 110195031A CN 201910416054 A CN201910416054 A CN 201910416054A CN 110195031 A CN110195031 A CN 110195031A
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- vomitoxin
- antidote
- intestinal mucosa
- degradation
- bifidobacterium adolescentis
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- LINOMUASTDIRTM-QGRHZQQGSA-N deoxynivalenol Chemical compound C([C@@]12[C@@]3(C[C@@H](O)[C@H]1O[C@@H]1C=C(C([C@@H](O)[C@@]13CO)=O)C)C)O2 LINOMUASTDIRTM-QGRHZQQGSA-N 0.000 title claims abstract description 70
- LINOMUASTDIRTM-UHFFFAOYSA-N vomitoxin hydrate Natural products OCC12C(O)C(=O)C(C)=CC1OC1C(O)CC2(C)C11CO1 LINOMUASTDIRTM-UHFFFAOYSA-N 0.000 title claims abstract description 70
- 239000000729 antidote Substances 0.000 title claims abstract description 64
- 210000004347 intestinal mucosa Anatomy 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 235000013557 nattō Nutrition 0.000 claims abstract description 46
- 241000186018 Bifidobacterium adolescentis Species 0.000 claims abstract description 42
- 241000191998 Pediococcus acidilactici Species 0.000 claims abstract description 41
- 230000015556 catabolic process Effects 0.000 claims abstract description 37
- 238000006731 degradation reaction Methods 0.000 claims abstract description 37
- 241000894006 Bacteria Species 0.000 claims abstract description 36
- 235000013339 cereals Nutrition 0.000 claims abstract description 30
- 241001465754 Metazoa Species 0.000 claims abstract description 9
- 230000000694 effects Effects 0.000 claims description 12
- 230000006378 damage Effects 0.000 claims description 10
- 239000003053 toxin Substances 0.000 claims description 7
- 231100000765 toxin Toxicity 0.000 claims description 7
- 230000002633 protecting effect Effects 0.000 claims description 6
- 230000008673 vomiting Effects 0.000 claims description 6
- 231100000614 poison Toxicity 0.000 claims description 5
- 241000726221 Gemma Species 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 206010047700 Vomiting Diseases 0.000 claims description 3
- 239000000470 constituent Substances 0.000 claims description 3
- 230000000593 degrading effect Effects 0.000 claims description 3
- 239000002574 poison Substances 0.000 claims description 3
- 239000003440 toxic substance Substances 0.000 claims description 3
- 230000006872 improvement Effects 0.000 claims description 2
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- 238000000034 method Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000018199 S phase Effects 0.000 claims 1
- 238000005457 optimization Methods 0.000 claims 1
- MBMQEIFVQACCCH-QBODLPLBSA-N zearalenone Chemical compound O=C1O[C@@H](C)CCCC(=O)CCC\C=C\C2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-QBODLPLBSA-N 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 230000002708 enhancing effect Effects 0.000 abstract description 4
- 231100000419 toxicity Toxicity 0.000 abstract description 4
- 230000001988 toxicity Effects 0.000 abstract description 4
- MBMQEIFVQACCCH-UHFFFAOYSA-N trans-Zearalenon Natural products O=C1OC(C)CCCC(=O)CCCC=CC2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-UHFFFAOYSA-N 0.000 abstract description 4
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 10
- 238000000855 fermentation Methods 0.000 description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 230000004151 fermentation Effects 0.000 description 8
- 230000000968 intestinal effect Effects 0.000 description 8
- 241000282412 Homo Species 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 235000014655 lactic acid Nutrition 0.000 description 5
- 239000004310 lactic acid Substances 0.000 description 5
- 108010062877 Bacteriocins Proteins 0.000 description 4
- 241001478240 Coccus Species 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 241000186000 Bifidobacterium Species 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000010564 aerobic fermentation Methods 0.000 description 3
- 230000003260 anti-sepsis Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 238000001694 spray drying Methods 0.000 description 3
- 241000193403 Clostridium Species 0.000 description 2
- 231100000678 Mycotoxin Toxicity 0.000 description 2
- 241000209149 Zea Species 0.000 description 2
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 description 2
- 239000002115 aflatoxin B1 Substances 0.000 description 2
- 229930020125 aflatoxin-B1 Natural products 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
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- 210000004698 lymphocyte Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000011700 menaquinone-7 Substances 0.000 description 2
- 239000002636 mycotoxin Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 210000004916 vomit Anatomy 0.000 description 2
- PFRQBZFETXBLTP-RCIYGOBDSA-N 2-[(2e,6e,10e,14e,18e)-3,7,11,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaen-1-yl]-3-methyl-1,4-dihydronaphthalene-1,4-dione Chemical compound C1=CC=C2C(=O)C(C/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)/CCC=C(C)C)=C(C)C(=O)C2=C1 PFRQBZFETXBLTP-RCIYGOBDSA-N 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 206010009900 Colitis ulcerative Diseases 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- 201000006704 Ulcerative Colitis Diseases 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000001062 anti-nausea Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000037180 bone health Effects 0.000 description 1
- 230000036996 cardiovascular health Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
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- 238000005260 corrosion Methods 0.000 description 1
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- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 210000002490 intestinal epithelial cell Anatomy 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 230000008944 intestinal immunity Effects 0.000 description 1
- 230000004675 intestinal mucosal permeability Effects 0.000 description 1
- 208000002551 irritable bowel syndrome Diseases 0.000 description 1
- 150000002561 ketenes Chemical class 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 229940086319 nattokinase Drugs 0.000 description 1
- 108010073682 nattokinase Proteins 0.000 description 1
- 235000021062 nutrient metabolism Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 235000016236 parenteral nutrition Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 235000020795 whole food diet Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/513—Adolescentes
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nutrition Science (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention discloses a kind of antidote preparation method degraded vomitoxin and protect intestinal mucosa.Antidote is composed of three kinds of bacterium solution dried objects of bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici according to different ratios.This preparation can be by the vomitoxin efficient degradation in cereal, degradation rate is up to 85%~95%, the degradation rate of this preparation improves 45%~180% compared with two component independent or any between its three kinds of components combines, and can control the vomitoxin in musty grain and limit 1mg/kg or less in safety.Antidote of the present invention is while efficient degradation vomitoxin, moreover it is possible to maintain beneficial bacteria of intestinal tract stable state, play the function of protection intestinal mucosa.Bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici are carried out reasonably combined Synergistic degradation vomitoxin by the present invention, and prevent vomitoxin and zearalenone from generating synergy enhancing toxicity, protect the safety of humans and animals intestinal mucosa.
Description
Technical field
The present invention relates to Tiny ecosystem fields, specifically, being related to bafillus natto, bifidobacterium adolescentis, lactic acid sheet
The antidote that the bacterium solution dried object of coccus is mixed according to certain component ratio, this antidote can be released efficiently in cereal
Vomitoxin, and intestinal mucosa can be protected.
Background technique
Vomitoxin is mainly produced by sickle-like bacteria, is widely present in cereal crops, is polluted when having eaten by mistake by vomitoxin
Food after, will lead to human body and vomiting, diarrhea, the poisoning symptoms such as anorexia occur, when serious or even will cause death.Youth bifid
Bacillus can be used for treating irritable bowel syndrome, can resist the effect of intestinal inflammatory such as ulcerative colitis illness recurrence, and
And by ours the study found that bifidobacterium adolescentis has the ability of degradation vomitoxin.There is Pediococcus acidilactici degradation to vomit
The ability of toxin is spat, the growth and breeding of pathogen can also be inhibited, adjusts intestinal microecology balance, there is enhancing intestinal immunity
Effect.Bafillus natto can inhibit the reproduction of pathogenic bacteria in a variety of enteron aisles, there is natural antiseptic agent, and natto
Bacillus can promote the growth of the anaerobic bacterias such as Bifidobacterium, Bacillus acidi lactici and clostridium, adjust intestinal microecology balance, natto
Bacillus can secret out of a variety of enzymes for promoting the absorption of Parenteral Nutrition substance, protect while enhancing digestive function
Intestinal mucosa epithelial cell and lymphocyte, prevent intestinal mucosa to be damaged, and protect the permeability of intestinal mucosa.
Application number CN201580045802.7 discloses a kind of bifidobacterium adolescentis for treat with potential impaired intestines
The disease of road barrier function.Patent of invention CN104046584A discloses a kind of bifidobacterium adolescentis bacteriocin and its production method
With special preparing strain, a kind of novel bifidobacterium bacteriocin is disclosed, antimicrobial spectrum is wide, and it is highly-safe, it is raw to can be used for wholefood
The exploitation of object preservative.Application number CN201710367900.3 discloses the preparation for containing Pediococcus acidilactici ZPA017 as feed
Additive feed weanling pig, be able to suppress harmful bacteria breeding, adjust intestinal microecology balance, have enhancing immunity of organisms,
Improve the effect of production performance.Application number CN201710360847.4 discloses a kind of Pediococcus acidilactici, and indicates that the bacterium can have
Effect improves the immunity of animal, improves intestinal microenvironment and promotes growth, has certain prebiotic effect.Jiang Fugui etc. (2017)
It was found that bafillus natto is mainly influenced the suction-operated of aflatoxin B1 by bacteria concentration, temperature and time is to absorption
Intensity does not make significant difference.Application number CN201710377231.8 disclose it is a kind of with produce MK-7 ability natto bud pole bacterium and
It is applied, and MK-7 is one of the active constituent of common farnoquinone, is easily absorbed by the human body, bone health can be enhanced, and is prevented
Osteoporosis inhibits angiosteosis, maintains arterial elasticity, and cardiovascular health is protected in prevention of arterial hardening.Liu Jinfang etc. (2019)
It was found that vomitoxin and aflatoxin B1 combined exposure can change the abundance of flora in mouse intestinal, it is extremely significant to reduce quasi- bar
The abundance of the beneficial bacteriums such as bacterium, deferrization bacillus, the extremely significant abundance for improving the harmful bacterias such as clostridium, conveyor screw.James is with undifferentiated
Caco-2 cell is external model, the reciprocation of a variety of mycotoxins is studied, as DON and ZEA synergy can promote cell
The generation of malonaldehyde causes cellular oxidation DNA damage, and more stronger than individually effect toxicity after two kinds of mycotoxin joints.
What the present invention studied focus on by by bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici bacterium solution dried object group
It closes, prepares antidote and efficiently release vomitoxin in cereal.In antidote, bafillus natto is able to suppress in environment
Harmful bacteria pollution is generated to antidote, there is good antisepsis, and it is able to maintain that bifidobacterium adolescentis and lactic acid
Piece coccus keeps the stability of height, and the Nattokinase etc. of secretion can also protect intestinal health.Through we have discovered that, solution
Bifidobacterium adolescentis in toxic agent has the ability of degradation vomitoxin, and the bacteriocin generated can also play protectant work
With, protection antidote safely and efficiently plays a role, and beneficial bacteria of intestinal tract is maintained to balance, promote nutrient metabolism and utilization,
And protect the stability of intestinal mucosa.Pediococcus acidilactici in antidote is then in the guarantor of bafillus natto and youth bacillus
Under shield, play the role of efficient degradation vomitoxin, prevent damage of the intestinal mucosa by toxin, enteron aisle is made to keep fit shape
State.Bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici bacterium solution dried object collective effect make antidote have efficiently solution
Bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici except vomitoxin ability, and in antidote can be in people and dynamic
It survives for a long time in object enteron aisle, and then vomitoxin bring secondary pollution can be prevented.And pass through our research indicate that, this hair
Bright antidote not only has efficient detoxication to vomitoxin, and also has efficiently to the zearalenone in cereal
Degradation, this antidote can prevent vomitoxin and zearalenone from generating combination, avoid two kinds of toxin superpositions
After generate stronger toxicity, thus it is safer it is efficient protection intestinal mucosa it is healthy and safe.This antidote safety non-pollution, can
Damage to prevent anti-nausea toxin to humans and animals intestinal mucosa, antidote can also be stablized in enteron aisle while high-efficiency detoxication
Probiotics flora inhibits the breeding of harmful bacteria in enteron aisle, repairs intestinal mucosa damage.
Summary of the invention
The present invention provides a kind of antidote preparation method degraded vomitoxin and protect intestinal mucosa.
Use of the invention bafillus natto (Bacillus subtilis natto) it is bafillus natto
CGMCC 1.1086, bifidobacterium adolescentis (Bifidobacterium adolescentis) it is bifidobacterium adolescentis CGMCC
1.2190 Pediococcus acidilactici (Pediococcus acidilactici) it is Pediococcus acidilactici CGMCC 1.123, it is purchased from China
General Microbiological Culture preservation administrative center, cereal are bought in the market of farm produce.
To achieve the above object, the technical solution adopted by the present invention are as follows:
A kind of antidote preparation method degraded vomitoxin and protect intestinal mucosa, mainly by bafillus natto, youth
Bifidobacterium, Pediococcus acidilactici bacterium solution dried object be composed according to different proportion.
Further, in the vomitoxin antidote of preparation protection intestinal mucosa, by bafillus natto, youth bifid bar
Bacterium, Pediococcus acidilactici bacterium solution dried object be composed, make bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici bacterium
Component ratio shared by liquid dried object be 20%~80%, the sum of be 100%.
A kind of degradation vomitoxin of the present invention simultaneously protects the antidote preparation method of intestinal mucosa the following steps are included: will receive
Beans bacillus, bifidobacterium adolescentis, Pediococcus acidilactici carry out liquid fermentation processing respectively, and the bacterium solution of acquisition is separately dried place
Reason, and by bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici bacterium solution dried object respectively with component ratio be 20%~
80%, the sum of be 100%, be combined and prepare vomitoxin solid antidote.
According to vomitoxin, there are the height of content in musty grain, when solid type antidote is mixed with cereal, Gu
Body antidote is added in musty grain according to the additive amount that mass percent is 0.05%~3%.
Bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici bacterium solution dried object individually or both combination is equal
The effect of efficient degradation vomitoxin cannot be obtained, can not play the efficient protecting effect to intestinal mucosa, the present invention by pair
Antidote optimizes improvement, combines the bacterium solution dried object of bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici, three
Person's interaction is common to improve the effect of antidote is to vomitoxin removing toxic substances efficiency and protection enteron aisle, and degradation rate is up to 85%~95%.
The efficiency of any one or two kinds of compositions in antidote of the present invention and its constituent in degradation vomitoxin
On compare, degradation rate improves 45%~180%.
Bafillus natto has good antisepsis in antidote of the present invention, humans and animals can be promoted to nutrients
Intestinal mucosa cell repair regeneration function is strengthened in the absorption of matter, safeguards intestinal mucosa permeability, and has degradation vomitoxin
Ability.
Bifidobacterium adolescentis can play the role of vomitoxin of degrading in antidote of the present invention, can generate bacteriocin, support
The pollution of harmful bacteria increases the resistance of antidote, is more adjustable intestinal flora in environment resistant to play the role of corrosion-resistant, promotees
It is bred into beneficial bacteria of intestinal tract, avoids damage of the vomitoxin to humans and animals intestinal mucosa.
Pediococcus acidilactici is able to suppress harmful bacteria in enteron aisle and breeds in antidote of the present invention, and can degrade and vomit poison
Element protects the normal morphology of intestinal epithelial cell and lymphocyte, avoids its damage by vomitoxin.
Utilize the content of national standard (GB 5009.111-2016) detection vomitoxin.
Antidote of the present invention not only has the function of vomitoxin of degrading, additionally it is possible to reduce vomitoxin to humans and animals intestines
The damage in road has the function of protecting intestinal mucosa.
The present invention provides a kind of antidote preparation method degraded vomitoxin and protect intestinal mucosa, vomits in efficient degradation
The healthy and safe of humans and animals intestinal mucosa can be protected while spitting toxin.The present invention has degradation vomitoxin and protects intestines
The antidote of mucous membrane is composed of the bacterium solution dried object of bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici.At this
In the antidote of invention, bafillus natto can degrade vomitoxin, have good antisepsis, be resistant to gastric acid guarantor
Shield antidote efficiently enters humans and animals enteron aisle, and bifidobacterium adolescentis and Pediococcus acidilactici can be made to keep the stabilization of height
Property, be conducive to bifidobacterium adolescentis and Pediococcus acidilactici efficient degradation vomitoxin, and it is thin into intestinal mucosa to secrete a variety of enzymatics
Born of the same parents, which are proliferated, to repair；Bifidobacterium adolescentis can degrade vomitoxin, protect intestinal mucosa not by the harm of vomitoxin；Lactic acid sheet
Coccus can be able to suppress harmful bacteria breeding, adjust with bifidobacterium adolescentis and bafillus natto Synergistic degradation vomitoxin
Intestinal microecology balance, maintains intestinal mucosal permeability.Of the invention has degradation vomitoxin and protects intestinal mucosa function
In antidote, the harm of vomitoxin can either be efficiently released, and protective barrier can be formed in enteron aisle, protects intestinal mucosa
Hold health status.Bafillus natto, bifidobacterium adolescentis and Pediococcus acidilactici in antidote can be protected for a long time in enteron aisle
High activity is held, can be degraded again to vomitoxin and avoids intestinal mucosa impaired.Studies have shown that antidote can not only
Vomitoxin in enough efficient degradation cereal, additionally it is possible to detoxify zearalenone, prevent vomitoxin red with corn
Mould ketenes, which generates synergy, enhances toxicity, so that a variety of toxin be avoided to combine the harm generated to intestinal mucosa.
Specific embodiment
Below with reference to embodiment, specific embodiment of the invention is described in further detail.Following embodiment is to use
The contents of the present invention are further illustrated, without limiting the scope of the invention.For related content according to the present invention
And the modification made, belong to the scope of the present invention.
Embodiment 1
Bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici are activated respectively, and are inoculated into respectively respectively suitably
Constant temperature incubation in liquid fermentation medium, wherein bifidobacterium adolescentis, Pediococcus acidilactici carry out anaerobic fermentation, natto gemma respectively
Bacillus carries out aerobic fermentation.Spray drying treatment is carried out to respective bacterium solution respectively after fermentation, collects junket natto gemma bar
Bacterium, bifidobacterium adolescentis, Pediococcus acidilactici bacterium solution dried object be combined, and according to bafillus natto, youth bifid bar
Bacterium, the bacterium solution dried object of Pediococcus acidilactici are uniformly mixed with component ratio (20%, 40%, 40%), to obtain having degradation vomiting poison
Element and the antidote for protecting intestinal mucosa function, it is spare.
The antidote of addition 0.05% into cereal, it is with blender that the exceeded cereal of vomitoxin and antidote is sufficiently mixed
It closes uniformly, the cereal for adding antidote is put into 30 DEG C of insulating boxs and is stood, after processing in 12 hours, detect remaining in cereal
Vomitoxin content, measure the degradation rate of vomitoxin up to 95%, be degraded to vomitoxin content in national standard limit 1mg/ safely
Within kg.
Embodiment 2
Bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici are activated respectively, and are inoculated into respectively respectively suitably
Constant temperature incubation in liquid fermentation medium, wherein bifidobacterium adolescentis, Pediococcus acidilactici carry out anaerobic fermentation, natto gemma respectively
Bacillus carries out aerobic fermentation.After fermentation collect bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici bacterium solution and point
Not carry out spray drying treatment, by the bacterium solution dried object handled well according to bafillus natto, bifidobacterium adolescentis, lactic acid sheet ball
Bacterium obtains the antidote of protection intestinal mucosa after mixing respectively with component ratio (20%, 20%, 60%).
Into cereal, the exceeded cereal of vomitoxin, is sufficiently mixed by the antidote of addition 0.1% with blender with antidote
Uniformly, the cereal for adding antidote is put into 30 DEG C of insulating boxs to stand, after 12 hours place, is detected remaining in cereal
Vomitoxin content.The bifidobacterium adolescentis bacterium solution dried object for adding 0.1% under the conditions of same treatment into cereal, at 12 hours
Vomitoxin degradation rate is detected after reason.Detection discovery antidote is 90% to the degradation rate of vomitoxin, bifidobacterium adolescentis bacterium solution
Dried object is 52% to the degradation rate of vomitoxin, and degradation rate improves 73.1%, vomitoxin in the cereal of detoxified dose of processing
Content drops within national standard safely limit 1mg/kg.
Embodiment 3 activates bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici respectively, and is inoculated with respectively
The constant temperature incubation into respective suitable liquid fermentation medium, wherein bifidobacterium adolescentis, Pediococcus acidilactici carry out anaerobism respectively
Fermentation, bafillus natto carry out aerobic fermentation.Bafillus natto, bifidobacterium adolescentis, lactic acid sheet are collected after fermentation
The bacterium solution of coccus simultaneously carries out spray drying treatment respectively, and the bacterium solution dried object handled well is double according to bafillus natto, youth
Discrimination bacillus, Pediococcus acidilactici obtain antidote respectively with component ratio (30%, 30%, 40%) after mixing, spare；And it will processing
Good bacterium solution dried object is mixed according to bafillus natto, Pediococcus acidilactici (50%, 50%), spare.
Into cereal, the exceeded cereal of vomitoxin, is sufficiently mixed by the antidote of addition 0.2% with blender with antidote
Uniformly, the cereal for adding antidote is put into 30 DEG C of insulating boxs to stand, after 12 hours place, is detected remaining in cereal
Vomitoxin content；Under the conditions of same treatment, 0.2% bafillus natto and the mixing of Pediococcus acidilactici are added into cereal
Bacterium solution dried object detects vomitoxin degradation rate after processing in 12 hours.Detection discovery antidote reaches the degradation rate of vomitoxin
93%, the mixed bacteria liquid dried object of bafillus natto and Pediococcus acidilactici is 63% to the degradation rate of vomitoxin, and degradation rate mentions
High by 47.6%, vomitoxin content drops within national standard safely limit 1mg/kg in the cereal of detoxified dose of processing.
Claims (6)
1. a kind of degradation vomitoxin and the antidote preparation method for protecting intestinal mucosa, which is characterized in that by natto gemma bar
Bacterium CGMCC 1.1086, bifidobacterium adolescentis CGMCC 1.2190, Pediococcus acidilactici CGMCC 1.123 bacterium solution dried object according to
Certain component ratio mixes.
2. a kind of antidote degraded vomitoxin and protect intestinal mucosa as described in claim 1, it is characterised in that antidote
When preparation, make bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici bacterium solution dried object shared by component ratio 20%~
80%, the sum of be 100%.
3. the antidote as described in claim 1~2, according to vomitoxin, there are the height of content in cereal, by solid type
When antidote is mixed with cereal, solid antidote is added in cereal according to mass percent for 0.05%~3%.
4. bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici bacterium solution dried object individually or both combination is not
The effect of efficient degradation vomitoxin can be obtained, the efficient protecting effect to intestinal mucosa can not be played, by antidote into
Row optimization improvement, combines the bacterium solution dried object of bafillus natto, bifidobacterium adolescentis, Pediococcus acidilactici, three's phase interaction
The effect of antidote is to vomitoxin removing toxic substances efficiency and protection enteron aisle is improved with common, and degradation rate is up to 85%~95%.
5. any one or the two kinds of compositions in the antidote as described in right 1~4, with its constituent are in degradation vomiting poison
It is compared in the efficiency of element, degradation rate improves 45%~180%.
6. the antidote of Claims 1 to 5 the method preparation not only has the function of vomitoxin of degrading, additionally it is possible to which reduction is vomitted
Damage of the toxin to humans and animals enteron aisle is spat, has the function of protecting intestinal mucosa.
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| CN102796694A (en) * | 2012-09-11 | 2012-11-28 | 国家粮食局科学研究院 | Engineering bacteria for efficiently degrading two fungal toxins and application |
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