CN110179085A - A kind of preparation method for the Chinese fiber crops seed bud being enriched with DHA and γ-aminobutyric acid - Google Patents
A kind of preparation method for the Chinese fiber crops seed bud being enriched with DHA and γ-aminobutyric acid Download PDFInfo
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- CN110179085A CN110179085A CN201910554479.6A CN201910554479A CN110179085A CN 110179085 A CN110179085 A CN 110179085A CN 201910554479 A CN201910554479 A CN 201910554479A CN 110179085 A CN110179085 A CN 110179085A
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- China
- Prior art keywords
- fiber crops
- chinese fiber
- crops seed
- aminobutyric acid
- dha
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- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 title claims abstract description 141
- 239000000835 fiber Substances 0.000 title claims abstract description 118
- 229960003692 gamma aminobutyric acid Drugs 0.000 title claims abstract description 71
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 title claims abstract description 69
- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 52
- 230000035784 germination Effects 0.000 claims abstract description 42
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000001035 drying Methods 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 241000218236 Cannabis Species 0.000 claims description 7
- QWCKQJZIFLGMSD-UHFFFAOYSA-N 2-Aminobutanoic acid Natural products CCC(N)C(O)=O QWCKQJZIFLGMSD-UHFFFAOYSA-N 0.000 claims description 6
- QWCKQJZIFLGMSD-GSVOUGTGSA-N D-alpha-aminobutyric acid Chemical compound CC[C@@H](N)C(O)=O QWCKQJZIFLGMSD-GSVOUGTGSA-N 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 3
- 230000007226 seed germination Effects 0.000 abstract description 4
- 238000007654 immersion Methods 0.000 abstract description 2
- MBMBGCFOFBJSGT-KUBAVDMBSA-N docosahexaenoic acid Natural products CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 63
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 60
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 8
- 235000001014 amino acid Nutrition 0.000 description 7
- 150000001413 amino acids Chemical class 0.000 description 7
- 229910021529 ammonia Inorganic materials 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 244000025254 Cannabis sativa Species 0.000 description 5
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 5
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 235000009120 camo Nutrition 0.000 description 5
- 235000005607 chanvre indien Nutrition 0.000 description 5
- 239000011487 hemp Substances 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 229940090949 docosahexaenoic acid Drugs 0.000 description 3
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- IJTNSXPMYKJZPR-UHFFFAOYSA-N parinaric acid Chemical compound CCC=CC=CC=CC=CCCCCCCCC(O)=O IJTNSXPMYKJZPR-UHFFFAOYSA-N 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 2
- YUFFSWGQGVEMMI-JLNKQSITSA-N (7Z,10Z,13Z,16Z,19Z)-docosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCCCC(O)=O YUFFSWGQGVEMMI-JLNKQSITSA-N 0.000 description 1
- TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 235000021294 Docosapentaenoic acid Nutrition 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- 241001300479 Macroptilium Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 201000009916 Postpartum depression Diseases 0.000 description 1
- 208000006399 Premature Obstetric Labor Diseases 0.000 description 1
- 206010036600 Premature labour Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- IJTNSXPMYKJZPR-WVRBZULHSA-N alpha-parinaric acid Natural products CCC=C/C=C/C=C/C=CCCCCCCCC(=O)O IJTNSXPMYKJZPR-WVRBZULHSA-N 0.000 description 1
- 229940124277 aminobutyric acid Drugs 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000002082 anti-convulsion Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000004641 brain development Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229930003827 cannabinoid Natural products 0.000 description 1
- 239000003557 cannabinoid Substances 0.000 description 1
- 229940065144 cannabinoids Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000020664 gamma-linolenic acid Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 230000000147 hypnotic effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 208000026440 premature labor Diseases 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 210000001525 retina Anatomy 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
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- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
- A23L25/20—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof consisting of whole seeds or seed fragments
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
A kind of preparation method for the Chinese fiber crops seed bud being enriched with DHA and γ-aminobutyric acid, it belongs to Chinese fiber crops seed germination field.After selected full, the unabroken Chinese fiber crops seed of the present invention cleans 3~4 times with clear water, it is put into germination tank；The temperature for adjusting germination tank is 23~25 DEG C, it is 1Kg:3~4L according to the solid-liquid ratio of Chinese fiber crops seed and pure water, pure water is injected into germination tank, then ozone is passed through into germination tank form Ozone Water, it is primary that water is changed after immersion 4~5h of Chinese fiber crops seed, impregnates Chinese fiber crops seed total time for water is discharged when 9~12h；The temperature of adjustment germination tank is 26~28 DEG C, carries out 10~12h of germination culture；After germination, the Chinese fiber crops seed of 3~5mm of radical bud length is chosen, is placed in drying in 35-45 DEG C of drying box, a kind of Chinese fiber crops seed bud for being enriched with DHA and γ-aminobutyric acid is made.The present invention improves the content of Chinese fiber crops seed DHA and γ-aminobutyric acid to a certain extent.
Description
Technical field
The invention belongs to Chinese fiber crops seed germination fields；More particularly to a kind of Chinese fiber crops seed bud for being enriched with DHA and γ-aminobutyric acid
Preparation method.
Background technique
Chinese fiber crops seed has very high nutritive value, and oil content is 25~35%, and protein content is 20~25%, carbon aquation
Closing object content is 20~30%, containing 10~15% non-solubility vitamin, and is rich in a large amount of minerals.Chinese sesame oil is big
The oil plant of water can be uniquely dissolved in nature, the unsaturated fatty acid content highest in all vegetable oil.Its mid-oleic is
10.48%, linoleic acid content 58.48%, linolenic acid content 17.78%, it is total to account for the 86.74% of total fatty acids.Sub- oil
For sour and linolenic ratio close to 3:1, this ratio is considered as optimal proportion needed for human homergy.But also contain
The gamma-Linolenic acid (GLA) and parinaric acid (SDA) that most of edible oils all do not contain.There are 21 kinds in Chinese fiber crops seed protein
Amino acid especially with content of glutamic acid highest, reaches 34.8mg/g, is secondly winter ammonia including 17 kinds of amino acid of needed by human body
Acid and arginine, content are respectively 19.8mg/g and 18.8mg/g.
γ-aminobutyric acid (GammaAminobut γ ricAcid, abbreviation GABA) is a kind of naturally occurring nonprotein
Amino acid is distributed widely in animal and plant body.All contain in plant such as Macroptilium, ginseng category, the seed of Chinese herbal medicine, rhizome and tissue fluid
There is GABA.Aminobutyric acid belongs to strong neural inhibitory aminoacid, has calmness, hypnosis, anticonvulsion, blood pressure lowering physiological action,
DHA, docosahexaenoic acid are commonly called as docosapentaenoic acid, are a kind of pair of very important unsaturated fatty acids of human body, belong to ω -3 insatiable hunger
With the important member in fatty acid families, promote fetal brain development, promote the maturation of retina light sensitive cells, it is thin to promote brain
Born of the same parents' development reduces postpartum depression, and treatment of cancer inhibits inflammation, reduces blood fat, prevention cardiovascular disease, it is silly to improve old man
It is slow-witted, inhibit influenza virus duplication, extends gestational period prevention premature labor, promote the absorption of PS (phosphatidylserine), anti-aging moisturizing
The effects of.
The upsurge for having started one research and development Chinese fiber crops seed in the world at present, has set up international hemp association for 1992
(IHA), many developed countries have also set up the hemp tissue of oneself in succession, and such as: the hemp Industry Association (HIA) in the U.S. adds and takes
Big hemp alliance (CHA), European hemp commercial guild (A, U.EU) of the European Community etc..External analysis and research show no matter
Which kind of purposes, which kind of kind Chinese fiber crops seed in all be free of any cannabinoids substance, have effects that safe healthcare to human body.
Summary of the invention
It is an object of the present invention to provide the preparation methods of a kind of enrichment DHA and the Chinese fiber crops seed bud of γ-aminobutyric acid.
The invention is realized by the following technical scheme:
The preparation method of a kind of enrichment DHA and the Chinese fiber crops seed bud of γ-aminobutyric acid, a kind of described enrichment DHA and γ-ammonia
The preparation method of the Chinese fiber crops seed bud of base butyric acid mainly includes the following steps:
After step 1, selected full, unabroken Chinese fiber crops seed clean 3~4 times with clear water, it is put into germination tank；
Step 2, the temperature for adjusting germination tank are 23~25 DEG C, are 1Kg:3 according to the solid-liquid ratio of Chinese fiber crops seed and pure water
Pure water is injected in~4L, Xiang Faya tank, then ozone is passed through into germination tank and forms Ozone Water, impregnate 4~5h of Chinese fiber crops seed
After to change water primary, impregnate and water be discharged when being 9~12h Chinese fiber crops seed total time；
Step 3, the temperature of adjustment germination tank are 26~28 DEG C, carry out 10~12h of germination culture；
After step 4, germination, the Chinese fiber crops seed of 3~5mm of radical bud length is chosen, is placed in 35-45 DEG C of drying box and does
It is dry, a kind of Chinese fiber crops seed bud for being enriched with DHA and γ-aminobutyric acid is made.
The preparation method of a kind of enrichment DHA of the present invention and the Chinese fiber crops seed bud of γ-aminobutyric acid, described in step 1
Chinese fiber crops seed is 1 year raw fructus cannabis seed.
The preparation method of a kind of enrichment DHA of the present invention and the Chinese fiber crops seed bud of γ-aminobutyric acid, described in step 1
Chinese fiber crops seed is fructus cannabis 1.
The preparation method of a kind of enrichment DHA of the present invention and the Chinese fiber crops seed bud of γ-aminobutyric acid, described in step 2
The concentration of Ozone Water is 0.4~0.8mg/l, changes after water that be passed through ozone to form the concentration of Ozone Water be 0.3~0.7mg/l.
The preparation method of a kind of enrichment DHA of the present invention and the Chinese fiber crops seed bud of γ-aminobutyric acid, Chinese fiber crops in step 2
The solid-liquid ratio of seed and pure water is 1Kg:3L, impregnates that change water after Chinese fiber crops seed 5h primary, and immersions Chinese fiber crops seed total time is 10h
When water is discharged.
The preparation method of a kind of enrichment DHA of the present invention and the Chinese fiber crops seed bud of γ-aminobutyric acid, germinate in step 3
The temperature of tank is 28 DEG C, and germination incubation time is 10h.
The preparation method of a kind of enrichment DHA of the present invention and the Chinese fiber crops seed bud of γ-aminobutyric acid, it is dry in step 4
The moisture content of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid afterwards is 5%.
The preparation method of a kind of enrichment DHA of the present invention and the Chinese fiber crops seed bud of γ-aminobutyric acid, it is dry in step 4
The alpha-aminobutyric acid content of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid afterwards is 409.8mg/l, and DHA content is
22.9mg/l。
The preparation method of a kind of enrichment DHA of the present invention and the Chinese fiber crops seed bud of γ-aminobutyric acid, germination tank used
For the CR-800 type intelligence sprouting machine of Nanjing Cheng Ru machinery Science and Technology Ltd. production.
The preparation method of a kind of enrichment DHA of the present invention and the Chinese fiber crops seed bud of γ-aminobutyric acid, are changed by germination
The structure of hempseed plant is handled using Chinese fiber crops seed as raw material through physiological activity method, is germinateed long to a normal bud, is made Chinese fiber crops seed
Middle part macromolecular substances resolve into the nutrients such as small-molecule substance, minerals, discharge and improve the organoleptic properties of Chinese fiber crops seed
And flavor, type and content that are abundant and improving the functional components in food especially improve the Chinese to a certain extent
The content of numb seed DHA and γ-aminobutyric acid.
The preparation method of a kind of enrichment DHA of the present invention and the Chinese fiber crops seed bud of γ-aminobutyric acid, can make DHA's
Content is increased to original 5 times, and the content of γ-aminobutyric acid is increased to original 6 times, and the method for the present invention step is simple, easily grasps
Make.
Detailed description of the invention
Fig. 1 is the photograph of a kind of enrichment DHA of one method of specific embodiment preparation and the Chinese fiber crops seed bud of γ-aminobutyric acid
Piece；
Fig. 2 be a kind of enrichment DHA of one method of specific embodiment preparation and the Chinese fiber crops seed bud of γ-aminobutyric acid it is single
Chinese fiber crops seed bud photo.
Specific embodiment
Specific embodiment 1:
The preparation method of a kind of enrichment DHA and the Chinese fiber crops seed bud of γ-aminobutyric acid, a kind of described enrichment DHA and γ-ammonia
The preparation method of the Chinese fiber crops seed bud of base butyric acid mainly includes the following steps:
After step 1, selected full, unabroken Chinese fiber crops seed clean 3 times with clear water, it is put into germination tank；
Step 2, the temperature for adjusting germination tank are 25 DEG C, are 1Kg:3L according to the solid-liquid ratio of Chinese fiber crops seed and pure water, to
Pure water is injected in germination tank, then ozone is passed through into germination tank and forms Ozone Water, impregnates that change water after Chinese fiber crops seed 5h primary,
Chinese fiber crops seed total time is impregnated for water is discharged when 10h；
Step 3, the temperature of adjustment germination tank are 28 DEG C, carry out germination culture 10h；
After step 4, germination, the Chinese fiber crops seed of 3~5mm of radical bud length is chosen, is placed in 35-45 DEG C of drying box and does
It is dry, a kind of Chinese fiber crops seed bud for being enriched with DHA and γ-aminobutyric acid is made.
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid described in present embodiment, step 1 institute
The Chinese fiber crops seed stated be 1 year raw fructus cannabis 1.
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid described in present embodiment, step 2 institute
The concentration for the Ozone Water stated is 0.8mg/l, changes after water that be passed through ozone to form the concentration of Ozone Water be 0.4mg/l.
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid described in present embodiment, in step 4
The moisture content of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid after drying is 5%.
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid described in present embodiment, in step 4
The alpha-aminobutyric acid content of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid after drying is 409.8mg/l, and DHA contains
Amount is 22.9mg/l.
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid described in present embodiment, with full-automatic
Amino-acid analyzer: DHA, the content of γ-aminobutyric acid are measured using National Standard Method GB5009.128.Instrument:
Full-automatic amino-acid analyzer: L-8800 splitter: IonExchangevolumn2622sc.pH4,6Film ×
60mm；Except ammonia column: IonExchangevolumn2650L.pH4,6mm × 60mm；Reaction column: 4.6mm × 40mm.
Agents useful for same: γ-aminobutyric acid standard items (>=99%), ninhydrin, ethyl alcohol.According to the full-automatic amino acid of L-8800
Analyzer user operation manual prepares B1, B2, B3, B4, B5 eluent and ninhydrin reagent R1, ninhydrin buffer solution respectively
R2.Gradient table is as shown in table 1:
1 gradient table of table/%
Separation condition:
Pump 1 flow velocity: 0.40mL/min；Pump 2 flow velocitys: 0.35mL/min；Column temperature: 57 DEG C of splitter, 135 DEG C of reaction column；Inspection
Survey wavelength: 1570nm sampling volume: 10 μ L.
The preparation of standard solution:
γ-aminobutyric acid (molecular weight 103.1) 10.31mg is accurately weighed, is dissolved and is determined with 0.02mol/L hydrochloric acid solution
Hold to 10mL, this solution concentration are as follows: 10 μm of ol/mL are diluted to 0.1 μm of ol/mL standard working solution using it.
The drafting of standard curve
By the γ-aminobutyric acid standard solution of 0.10mol/mL, under above-mentioned analysis condition successively sample introduction 5.0,7.0,
10.0,15.0,20.0 μ L make linear regression with γ-aminobutyric acid sample volume x (μ g) and peak area y, obtain regression equation.
Sample treatment:
The germination Chinese fiber crops seed sample that 3~5g has been crushed is weighed, is placed in 100mL volumetric flask, adds appropriate 60% ethanol solution,
6h is extracted in 55 DEG C of waters bath with thermostatic control, shakes up sample 30S every 30min therebetween.It is settled to 100mL after being cooled to room temperature, takes 2OmL
Extracting solution is set in people's 50mL centrifuge tube, is centrifuged 10min with 4000r/min, is taken supernatant through 0.45 μm of membrane filtration, take 10 μ L into
Sample measures the peak area of the sample.Resulting peak area value is substituted into the regression equation that titer is formulated, the sample can be obtained
The content of γ-aminobutyric acid.
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid described in present embodiment, the germination of Chinese fiber crops
Comparatively fast, it is generally completed in 48h, and speed of germination is uneven, therefore using Chinese fiber crops seed germination (radical bud) length as variable.Respectively to difference
The Chinese fiber crops of radical bud length (l-2mm, 4-5mm, 7-8mm, ll-12mm, 14-15mm, 17-18mm, 21-22mm, 25mm or more) take
Sample (is rounded a Chinese fiber crops), and sample is placed in 45 DEG C of drying boxes and is dried to moisture 5% or so, cooling, is ground into pulverizer
Powder is crossed 40 meshes, is saved backup.After test the result shows that, the Chinese fiber crops seed germination after DHA, (docosahexaenoic acid) and γ-ammonia
The content of base butyric acid can dramatically increase.But radical bud length is different, the content of DHA and γ-aminobutyric acid is also different, with root
The increase DHA and alpha-aminobutyric acid content of bud length also will increase, and reach peak when radical bud length is 3~5mm, radical bud is long
After degree is more than 3~5mm, the content of DHA and γ-aminobutyric acid will decline, and obtain the Chinese fiber crops seed that radical bud length is 3~5mm,
It can make DHA, (docosahexaenoic acid) and alpha-aminobutyric acid content content reach peak, as shown in table 2:
2 Chinese fiber crops seed of table compares before and after the processing
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid described in present embodiment, can make
The content of DHA is increased to original 5 times, and the content of γ-aminobutyric acid is increased to original 6 times, and method and step is simple, easily grasps
Make.
Specific embodiment 2:
The preparation method of a kind of enrichment DHA and the Chinese fiber crops seed bud of γ-aminobutyric acid, a kind of described enrichment DHA and γ-ammonia
The preparation method of the Chinese fiber crops seed bud of base butyric acid mainly includes the following steps:
After step 1, selected full, unabroken Chinese fiber crops seed clean 3~4 times with clear water, it is put into germination tank；
Step 2, the temperature for adjusting germination tank are 23~25 DEG C, are 1Kg:3 according to the solid-liquid ratio of Chinese fiber crops seed and pure water
Pure water is injected in~4L, Xiang Faya tank, then ozone is passed through into germination tank and forms Ozone Water, impregnate 4~5h of Chinese fiber crops seed
After to change water primary, impregnate and water be discharged when being 9~12h Chinese fiber crops seed total time；
Step 3, the temperature of adjustment germination tank are 26~28 DEG C, carry out 10~12h of germination culture；
After step 4, germination, the Chinese fiber crops seed of 3~5mm of radical bud length is chosen, is placed in 35-45 DEG C of drying box and does
It is dry, a kind of Chinese fiber crops seed bud for being enriched with DHA and γ-aminobutyric acid is made.
Specific embodiment 3:
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid according to specific embodiment two,
Chinese fiber crops seed described in step 1 is 1 year raw fructus cannabis seed.
Specific embodiment 4:
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid according to specific embodiment two,
Chinese fiber crops seed described in step 1 is fructus cannabis 1.
Specific embodiment 5:
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid according to specific embodiment two,
The concentration of Ozone Water described in step 2 is 0.4~0.8mg/l, change after water be passed through ozone formed the concentration of Ozone Water be 0.3~
0.7mg/l。
Specific embodiment 6:
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid according to specific embodiment two,
The solid-liquid ratio of Chinese fiber crops seed and pure water is 1Kg:3L in step 2, impregnates that change water after Chinese fiber crops seed 5h primary, impregnates Chinese fiber crops seed
Total time, water was discharged when being 10h.
Specific embodiment 7:
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid according to specific embodiment two,
The temperature of germination tank is 28 DEG C in step 3, and germination incubation time is 10h.
Specific embodiment 8:
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid according to specific embodiment two,
In step 4 it is dry after a kind of enrichment DHA and the moisture content of Chinese fiber crops seed bud of γ-aminobutyric acid be 5%.
Specific embodiment 9:
The preparation method of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid according to specific embodiment two,
In step 4 it is dry after a kind of enrichment DHA and the alpha-aminobutyric acid content of Chinese fiber crops seed bud of γ-aminobutyric acid be 409.8mg/
L, DHA content 22.9mg/l.
Claims (8)
1. the preparation method of a kind of enrichment DHA and the Chinese fiber crops seed bud of γ-aminobutyric acid, it is characterised in that: a kind of enrichment
The preparation method of DHA and the Chinese fiber crops seed bud of γ-aminobutyric acid mainly includes the following steps:
After step 1, selected full, unabroken Chinese fiber crops seed clean 3~4 times with clear water, it is put into germination tank；
Step 2, the temperature for adjusting germination tank are 23~25 DEG C, are 1Kg:3~4L according to the solid-liquid ratio of Chinese fiber crops seed and pure water,
Pure water is injected into germination tank, then ozone is passed through into germination tank and forms Ozone Water, change water after impregnating 4~5h of Chinese fiber crops seed
Once, Chinese fiber crops seed total time is impregnated for water is discharged when 9~12h；
Step 3, the temperature of adjustment germination tank are 26~28 DEG C, carry out 10~12h of germination culture；
After step 4, germination, the Chinese fiber crops seed of 3~5mm of radical bud length is chosen, is placed in drying in 35-45 DEG C of drying box,
A kind of Chinese fiber crops seed bud for being enriched with DHA and γ-aminobutyric acid is made.
2. the preparation method of a kind of enrichment DHA according to claim 1 and the Chinese fiber crops seed bud of γ-aminobutyric acid, feature
Be: Chinese fiber crops seed described in step 1 is 1 year raw fructus cannabis seed.
3. the preparation method of a kind of enrichment DHA according to claim 2 and the Chinese fiber crops seed bud of γ-aminobutyric acid, feature
Be: Chinese fiber crops seed described in step 1 is fructus cannabis 1.
4. the preparation method of a kind of enrichment DHA according to claim 1 and the Chinese fiber crops seed bud of γ-aminobutyric acid, feature
Be: the concentration of Ozone Water described in step 2 is 0.4~0.8mg/l, changes after water to be passed through ozone and form the concentration of Ozone Water and is
0.3~0.7mg/l.
5. the preparation method of a kind of enrichment DHA according to claim 3 and the Chinese fiber crops seed bud of γ-aminobutyric acid, feature
Be: the solid-liquid ratio of Chinese fiber crops seed and pure water is 1Kg:3L in step 2, impregnates that change water after Chinese fiber crops seed 5h primary, impregnates the Chinese
Numb seed total time, water was discharged when being 10h.
6. the preparation method of a kind of enrichment DHA according to claim 1 and the Chinese fiber crops seed bud of γ-aminobutyric acid, feature
Be: the temperature for the tank that germinates in step 3 is 28 DEG C, and germination incubation time is 10h.
7. the preparation method of a kind of enrichment DHA according to claim 1 and the Chinese fiber crops seed bud of γ-aminobutyric acid, feature
Be: the moisture content of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid after dry in step 4 is 5%.
8. the preparation method of a kind of enrichment DHA according to claim 1 and the Chinese fiber crops seed bud of γ-aminobutyric acid, feature
Be: the alpha-aminobutyric acid content of the Chinese fiber crops seed bud of a kind of enrichment DHA and γ-aminobutyric acid after dry in step 4 is
409.8mg/l, DHA content 22.9mg/l.
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