CN110178991A - A kind of nutrition regulation method promoting lamb intestinal growth - Google Patents
A kind of nutrition regulation method promoting lamb intestinal growth Download PDFInfo
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- CN110178991A CN110178991A CN201910383990.4A CN201910383990A CN110178991A CN 110178991 A CN110178991 A CN 110178991A CN 201910383990 A CN201910383990 A CN 201910383990A CN 110178991 A CN110178991 A CN 110178991A
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- lamb
- folic acid
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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Abstract
The present invention provides a kind of nutrition regulation method for promoting lamb intestinal growth, specifically: folic acid is made an addition in sheep daily ration, for feed from breeding to the pregnancy ewes during lambing, promotes new life lamb intestinal growth.Method of the invention can effectively improve newborn lamb small intestine weight, small intestine weight accounts for Slaughter weight index, have important facilitation to the digestibility and utilization of nutriment in breast milk after being born to lamb；Newborn lamb villi height, muscle layer thickness morphologically can be improved, newborn lamb intestinal development can be improved in mRNA level in-site, the genes relative expression quantity such as EGF, IGF-1, BCL-2, PEPT1, LCT, CLAUDIN-1 can be improved, reduce BAX relative expression quantity, improve newborn lamb intestinal development comprehensively in terms of development, apoptosis, digestion and absorption and barrier function four, the healthy growth for guaranteeing lamb, makes the congenital acquisition advantage of lamb intestinal development；It is convenient to operate, remarkable in economical benefits.
Description
Technical field
The present invention relates to Animal Sciences, and in particular to a kind of nutrition regulation method for promoting lamb intestinal growth.
Background technique
Folic acid (Folic Acid), i.e. vitamin B9, it is water as a kind of essential nutrients in humans and animals growth and development
One of dissolubility B family vitamin participates in one carbon unit metabolism, with amino acid metabolism, DNA synthesis and reparation, and gene modification
Deng there is also close ties.Folic acid influences DNA synthesis and methionine circulation, participates in reproduction metabolic pathway and lactoprotein synthesis；Cause
This before gestation and includes lactation period after gestation, and folic acid is all very important.
Studies have found that the Supplement of folic acid in gestational period rat daily ration, can significantly improve offspring's Birth weight (Cho etc.,
2013).Because folic acid plays an important role in the metabolic process of purine, pyrimidine and amino acid, so embryo DNA, RNA can be promoted
And the biosynthesis of various protein, so that the growth and development of formation and fetus to embryo has an impact.
The development of small intestine and function are most important to the growth of animal and health, in the digestion and absorption of nutriment, energy
It plays an important role in terms of the consumption and immunocompetence of amount and nutriment.Small intestine is not only the master of nutrient digestion absorption
Place is wanted, and since its metabolic activity is high, turnover is fast and the primary warehouse of energy and nutriment.Enteron aisle growth and development
When in utero the variation in bad border is more sensitive in early days, and all the life be in dynamic change, gestational period enteron aisle it is good develop to it
Growth and development and health after offspring born have very important influence, are lamb survival rate, growth and development and production effect
Good physiological Foundations have all been established in rate raising.
The important as precursors object that folic acid is synthesized as nucleotide, horizontal relationship the growth and development of each tissue of body, especially
It is the stage developed rapidly in pregnant middle and later periods small intestine, and quick cell Proliferation needs a large amount of nucleotide to participate in.The study found that
Fetus and birth rear intestinal specific gene DNA methylation are changed in female rat daily ration when folic acid deficiency, influences intestinal health
(Mckay etc., 2011), and it is straight can to significantly reduce offspring's knot by influencing hindgut genomic DNA methylation level to mother's Supplement of folic acid
The incidence (Ly A etc., 2011) of intestinal cancer.
Although the natural folate content in daily ration is seldom, nutrition of classics ruminating thinks that cud itself can synthesize leaf
Acid, therefore the gestational period does not need additionally to supplement, but more and more tests prove the leaf of gestational period ruminant itself synthesis
Acid is not able to satisfy fetus and the parent development this numerous kind of height of needs, especially sheep, the folic acid and daily ration of cud synthesis
In natural folic acid do not ensure that the needs of polyembryony lamb development.
Summary of the invention
For defect present in the prior art and deficiency, one aspect of the present invention provides a kind of promotion lamb intestinal growth
Nutrition regulation method, specifically, using be added to folic acid basal diet feed pregnancy ewes.
In the above-mentioned technical solutions, the pregnancy ewes are through producing ewe.
Further, in the above-mentioned technical solutions, the folic acid is coating folic acid.
Preferably, in the above-mentioned technical solutions, the cud percent of pass of the coating folic acid is 90-95%, small intestine release rate
For 82-88%.
Still further, in the above-mentioned technical solutions, in every kilogram of basal diet dry matter, the additive amount of the folic acid
For 16-32mg.
Further, in the above-mentioned technical solutions, the feeding period of the basal diet for being added to folic acid is from matching
It plants to lambing.
Preferably, in the above-mentioned technical solutions, the feed mode of the basal diet for being added to folic acid is sooner or later each raises
It feeds primary.
Also further, in the above-mentioned technical solutions, the pregnancy ewes are sheep.
Another aspect of the present invention provides a kind of sheep daily ration for promoting newborn lamb intestinal growth, and the sheep daily ration includes base
Plinth daily ration and folic acid add folic acid described in 16-32mg in the dry matter of every kilogram of basal diet.
The present invention makes an addition to folic acid in sheep daily ration, feeds gravidic lambing ewe, provides a kind of promotion lamb intestine
The nutrition regulation method of road development has as follows compared with prior art
The utility model has the advantages that
(1) method of the invention can effectively improve newborn lamb small intestine weight, small intestine weight accounts for Slaughter weight index,
For having important facilitation to the digestibility and utilization of nutriment in breast milk after lamb birth；
(2) newborn lamb villi height, muscle layer thickness morphologically can be improved in method of the invention, in mRNA level in-site
On newborn lamb intestinal development can be improved the genes phase such as EGF, IGF-1, BCL-2, PEPT1, LCT, CLAUDIN-1 can be improved
To expression quantity, BAX relative expression quantity is reduced, is improved comprehensively in terms of development, apoptosis, digestion and absorption and barrier function four newborn
Lamb intestinal development guarantees the healthy growth of lamb, makes the congenital acquisition advantage of lamb intestinal development；
(3) provided by the present invention make an addition to folic acid in sheep daily ration is used to feed from breeding to the gestation mother during lambing
Sheep promotes the method for newborn lamb intestinal growth to operate convenient, remarkable in economical benefits, and practical significance is great.
Specific embodiment
Combined with specific embodiments below, specific embodiments of the present invention will be described in further detail.
Following embodiment is merely to illustrate the present invention, the protection scope being not intended to limit the invention.
Experimental method used in following embodiment is conventional method unless otherwise specified.
Material used in following embodiment, reagent etc., are commercially available unless otherwise specified.
It is illustrated by taking the sheep kind of high various tire as an example in embodiment, but applicants have found that, other types
The especially single tire kind of sheep be easier to obtain similar effect.
Embodiment 1
The present embodiment illustrates method of the invention using newborn lamb as research object.
Choose same strain, weight it is close and it is healthy produced heat and waited for sheep dam 120, and completed in three days
It breeds work (sooner or later respectively with primary), is randomly divided into 3 groups, respectively control group, processing group 1 and processing group after the completion of breeding immediately
2, the daily ration of the additional addition different level folic acid of feeding, maintains each processing feed intake identical, specifically as shown in table 1 respectively.
The different group daily rations of table 1 add folate level
Remarks: DMI is dry matter intake in table 1.
It after the completion of breeding, is fed according to processing, 16-18 age in days carries out trying anti-feelings, rejects non-pregnant female；With finishing
Cheng Hou, 30 ages in days further detect breeding as a result, rejecting non-pregnant female again according to B ultrasound testing result using B ultrasound, finally produce
The dam number of lamb is 85: control group 27, handles 1 group 32, handles 2 groups 26.
Lamb birth after, chosen respectively from every group immediately health, weight close to average weight, type of birth be twin lamb(s) and three
The lamb of lamb each 6 are butchered, and after opening abdominal cavity, acquire duodenum and jejunum leading portion morphology sample and molecule sample immediately
Product are respectively used to measurement morphological index and mRNA expression, specific as shown in table 2.
The lamb intestine road exponential case of 2 control group of table and processing group
As can be seen from Table 2, the small intestine of lamb in 1 group and 2 groups of processing/that Slaughter weight percentage compares control group is obvious is handled
Increase, 6.96% and 10.13% has specially been respectively increased, this is proved after adding folic acid in the daily ration of ewe at gestation period, to new
Raw lamb intestinal growth significantly facilitates effect.
3 control group of table and processing group enteron aisle phenotype dual factors the result of multiple comparisons
Result of study shows to add 16 and 32mg/kg.DMI folic acid in gestational period dam daily ration, can significantly improve 12 and refer to
Villi height, muscle layer thickness and jejunum muscle layer thickness, type of birth and folic acid concentration have significant interaction to Duodenal villi
Effect, folate level acts on Duodenal villi with extremely significant raising, in twin lamb(s) type, 1 duodenum suede of processing group
430.96 μm of hair height, 373.32 μm of 2 villi height of processing group, 304.64 μm of control group villi height, compared with 1 He of processing group
2 are respectively increased 41.47% and 22.54%；270.54 μm of 1 muscle layer thickness of processing group, 331.17 μm of 2 muscle layer thickness of processing group are right
According to 200.42 μm of group muscle layer thickness, processing group 1 and 2 improves 34.99% and 65.24% compared with control group respectively.Folate level is to sky
Myenteron thickness, which generates conspicuousness, to be influenced, 1 160.32 μm of jejunum muscle layer thickness of processing group, 2 jejunum muscle layer thickness of processing group
160.43 μm, 126.77 μm of thickness of control group jejunum muscle layer, 26.47% and 26.55% is respectively increased compared with processing group 1 and 2.To sum up
It can illustrate, after adding folic acid in the daily ration of ewe at gestation period, effect is significantly facilitated to the development of newborn lamb Intestinal Morphology,
Improve lamb production efficiency.
4 control group of table and processing group enteron aisle gene expression dual factors the result of multiple comparisons
5 control group of table and processing group enteron aisle gene expression dual factors the result of multiple comparisons
6 control group of table and processing group enteron aisle gene expression dual factors the result of multiple comparisons
Table 4, table 5 and table 6 are the comparisons of control group and the expression of processing group lamb intestine dao gene, and processing result shows gestation
In phase ewe daily ration add 16 and 32mg/kg.DMI folic acid, can significantly improve newborn lamb duodenum EGF, BCL-2,
CLAUDIN-1, ZO-1, SI, OCCLUDIN gene relative expression quantity and jejunum LCT gene relative expression quantity reduce jejunum BAX base
Because of relative expression quantity.
Specific effect is as follows: EGF relative expression quantity processing group 1 is improved compared with control group in duodenum twin lamb(s) type
197.5%；Relatively control group improves 127.65 and 96.8% respectively for BCL-2 relative expression quantity processing group 1 and processing group 2；12 refer to
SI relative expression quantity processing group 1 improves 65.92% compared with control group in intestines twin lamb(s) type, SI relative expression quantity processing in three lamb types
Group 2 improves 590.90% compared with control group；OCCLUDIN relative expression quantity processing group 2 is improved compared with control group in three lamb types
239.78%；CLAUDIN-1 relative expression quantity processing group 2 improves 132.81% compared with control group in three lamb types；In three lamb types
Relatively control group improves 73.64% and 273.73% respectively for ZO-1 relative expression quantity processing group 1 and processing group 2；Jejunum twin lamb(s) type
Middle LCT relative expression quantity processing group 1 improves 136.91% compared with control group；BAX relative expression quantity processing group 2 in jejunum twin lamb(s) type
60.75% is reduced compared with control group.This is proved after adding folic acid in the daily ration of ewe at gestation period, peomotes newborn lamb small intestine
Growth factor is secreted, intestinal epithelial cell apoptosis is reduced, improves small intestine epithelium nutriment transport vehicle protein gene and digestion
The expression of enzyme gene improves small intestine abilities of digestive and absorption, improves intestinal mucosal barrier function, improves intestinal permeability, to lamb
Sheep intestinal growth significantly facilitates effect
Therefore, Comprehensive research result adds effective coating folic acid energy of 16~32mg/kg.DMI in ewe at gestation period daily ration
Newborn lamb intestinal development is enough improved, makes the congenital acquisition advantage of lamb intestinal development, guarantees the healthy growth of lamb.
Although above having used general explanation, specific embodiment and processing, the present invention is made to retouch in detail
It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Range.
Claims (9)
1. a kind of nutrition regulation method for promoting lamb intestinal growth, which is characterized in that using the basal diet for being added to folic acid
Feed pregnancy ewes.
2. nutrition regulation method according to claim 1, which is characterized in that the pregnancy ewes are through producing ewe.
3. nutrition regulation method according to claim 1 or 2, which is characterized in that the folic acid is coating folic acid.
4. nutrition regulation method according to claim 3, which is characterized in that it is described coating folic acid cud percent of pass be
90-95%, small intestine release rate are 82-88%.
5. nutrition regulation method according to claim 1-4, which is characterized in that in every kilogram of basal diet dry
In matter, the additive amount of the folic acid is 16-32mg.
6. nutrition regulation method according to claim 1-5, which is characterized in that the basis for being added to folic acid
The feeding period of daily ration is from breeding to lambing.
7. nutrition regulation method according to claim 6, which is characterized in that the feeding of the basal diet for being added to folic acid
The mode of feeding is that each feeding is primary sooner or later.
8. nutrition regulation method according to claim 6 or 7, which is characterized in that the pregnancy ewes are sheep.
9. a kind of sheep daily ration for promoting newborn lamb intestinal growth, which is characterized in that including basal diet and folic acid, every kilogram of institute
It states and adds folic acid described in 16-32mg in the dry matter of basal diet.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910383990.4A CN110178991A (en) | 2019-05-09 | 2019-05-09 | A kind of nutrition regulation method promoting lamb intestinal growth |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910383990.4A CN110178991A (en) | 2019-05-09 | 2019-05-09 | A kind of nutrition regulation method promoting lamb intestinal growth |
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| CN110178991A true CN110178991A (en) | 2019-08-30 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107279497A (en) * | 2017-07-18 | 2017-10-24 | 南京农业大学 | Application of the Aspartame in young age ruminant intestinal development, the small intestinal barrier function of enhancing is promoted |
| CN108576416A (en) * | 2018-04-03 | 2018-09-28 | 中国农业大学 | Application of the folic acid in improving dam lambing percentage |
-
2019
- 2019-05-09 CN CN201910383990.4A patent/CN110178991A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107279497A (en) * | 2017-07-18 | 2017-10-24 | 南京农业大学 | Application of the Aspartame in young age ruminant intestinal development, the small intestinal barrier function of enhancing is promoted |
| CN108576416A (en) * | 2018-04-03 | 2018-09-28 | 中国农业大学 | Application of the folic acid in improving dam lambing percentage |
Non-Patent Citations (1)
| Title |
|---|
| JILL A.MCKAY ET AL.: "Maternal folate supply and sex influence gene-specific DNA methylation in the fetal gut", 《MOLECULAR NUTRITION & FOOD RESEARCH》 * |
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