CN110170047A - A kind of antibacterial ointment of glusulase - Google Patents
A kind of antibacterial ointment of glusulase Download PDFInfo
- Publication number
- CN110170047A CN110170047A CN201910465046.3A CN201910465046A CN110170047A CN 110170047 A CN110170047 A CN 110170047A CN 201910465046 A CN201910465046 A CN 201910465046A CN 110170047 A CN110170047 A CN 110170047A
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- China
- Prior art keywords
- ointment
- glusulase
- parts
- antibacterial
- raw material
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Abstract
The present invention relates to a kind of antibacterial ointment of glusulase, the raw material of the antibacterial ointment of glusulase includes ointment bases, snail enzyme extract, earth-worm extractive as raw material and Chinese medicine composition ointment；The raw material of the Chinese medicine composition ointment is Chinese gall, frutus cnidii, Cortex Phellodendri, kuh-seng, wild chrysanthemum, the fruit of summer cypress, smilax and black snake.The antibacterial ointment of glusulase provided by the invention contains the snails such as the glusulase of bacteriostatic activity and extracts bioactive substance, and protein content is high, and inhibitory potency effect is more preferable.The plaster that collocation in conjunction with earth-worm extractive as raw material and Chinese medicine composition is prepared can promote dyskeratosis cytothesis, prevents and treats skin unicorn caused by dry and cracked, decortication and hyperkeratosis hyperplasia and considers to be worth doing.Preparation is simple with ointment for preferred snail enzyme extract preparation, and stability is good, can be used for industrial production.
Description
Technical field
The invention belongs to biological product technical fields, are related to a kind of antibacterial ointment of glusulase.
Background technique
Antimicrobial compound is a kind of active material with antibacterial action.Bacterium infection once seriously threatened the life of the mankind
It deposits and develops.Using Fleming discovery penicillin as the beginning, people start constantly to find from microbial secondary metabolite numerous
Effective antibacterial substance simultaneously develops semisynthetic antibiotics.The antibacterial of numerous species can be extracted out of microbial body
Matter, it becomes the main source for obtaining antibacterial material.In addition, antibacterial material can also derive from plant, animal etc..Plant is certainly
In right boundary's life and growth and development process, it is subjected to infecting for pathogen.Plant can pass through the expression institute of intracorporal disease-resistant gene
The antibacterial material of generation, the i.e. product of its secondary metabolism resist the infringement of pathogen.Plants antimicrobial compound can be divided into group again
Become second nature antimicrobial compound and inductivity antimicrobial compound, they have desinsection, sterilization, weeding, kill rat destruction and adjust biology life
Long and development isoreactivity, such as S-Ethyl ethylthio sulfonate, matrine, citral, protective plant protecting agent.And animal and its appendicular antibacterial material are ground
Study carefully and illustrates its wide application prospect for the development and utilization of natural products.Especially nucleoprotamine, tussah antibacterial skin and yolk
The kinds such as immunoglobulin, propolis, chitosan and lysozyme can be used as from the natural antimicrobial substance of animal prepares drug, makeup
Ingredient in product or health care product.
Snail belongs to Mollusca, Gastropoda, there are many kinds of class is edible, such as escargot (snails), flower garden snail and Ma
Nao snail etc..Its source is simple, and breeding is very fast, and raising is convenient, can will be fine based on the rich cellulose-containing vegetable matter of intake
Energy substance of the glucose that dimension element hydrolysis generates as growth and development.Carnis Limax is consolidated divided by high protein, low fat, without gallbladder
Alcohol, meat are fine and smooth, taste is fresh and tender palatable outer, and more than 20 enzymes are contained in snail glandula digestive, mainly have fibre with application value
Tie up plain enzyme, pectase, alpha-amylase, 1,4- β-D mannose restriction endonuclease, β-D glucose hydrolase, alpha-Mannosidase, sweet dew
Sugar alcohol oxidizing ferment, aminotransferase and cholinesterase, wherein with cellulase (10%), pectase (10%), alpha-amylase
(8%) at most.Glusulase is a kind of of great value enzyme, it can be used for the broken of yeast cell wall, and it is raw to be widely used in cell
The research of object and genetic engineering, also can be used as feed addictive, to improve the digestibility of feed；It also is used as fruit juice
Clarification, orange take off tunicle, jam production etc..China's heliculture industry and the processing industry of Carnis Limax have developed rapidly at present, and big portion
Snail internal organ are divided to be thrown away as waste.Do not influence snail it is edible in the case where, how to make full use of the internal organ of snail discarded
Object extracts glusulase and other protein substances, is both conducive to the comprehensive utilization of snail, increases benefit, and be conducive to environmental protection.How is research
It is with important application prospects with antibacterial substance is extracted in affluent resources internal organ waste.
Earthworm contains a large amount of protein and fat, and nutritive value is very high, is excellent protein feed and food.From earthworm
The active constituent (such as Lumbrokinase and antibacterial peptide) extracted in earthworm has been developed that patent medicine, tool thrombus dissolving, anticoagulation, antitumor, suppression
The effects of bacterium.It can reach the effect of disease-control using coelomic fluid of earthworm processing vegetables and obtain environmentally protective food.Earthworm can incite somebody to action
Reach nuisanceless after pollutant in soil processing.Therefore, collect medicinal material, feed, food and decontamination is antifouling etc. is economical and environmentally friendly worth
Earthworm, great development prospect.
Summary of the invention
In view of this, the ointment is that biological products mention the purpose of the present invention is to provide a kind of glusulase antibacterial ointment
Object is taken, it is mild to skin, to dermatitis (nerve, anaphylaxis), psoriasis, brothers' favus of the scalp, tinea pedis and manus, tinea versicolor, eczema, nettle
Rash has certain clinical effectiveness.
In order to achieve the above objectives, the invention provides the following technical scheme:
1. a kind of antibacterial ointment of glusulase, the raw material of the antibacterial ointment of glusulase includes ointment bases, glusulase extraction
Object, earth-worm extractive as raw material and Chinese medicine composition ointment；The raw material of the Chinese medicine composition ointment be Chinese gall, frutus cnidii, Cortex Phellodendri,
Kuh-seng, wild chrysanthemum, the fruit of summer cypress, smilax and black snake.
Further, according to the mass fraction, in the raw material of the antibacterial ointment of the glusulase, 100 parts of ointment bases, glusulase
2-3 parts of extract, 1-2 parts of earth-worm extractive as raw material, 20-30 parts of Chinese medicine composition ointment.
Further, according to the mass fraction, in the raw material of Chinese medicine composition ointment: 20-25 portions of Chinese galls, 10-15 portions of snakes
Machine tool, 100-130 portions of Cortex Phellodendris, 10-15 parts of kuh-sengs, 10-15 parts of wild chrysanthemums, the 10-12 parts of fruit of summer cypress, 20-25 portions of smilaxs and 10-
15 parts of black snakes.
Further, after the snail enzyme extract adds buffer to be homogenized for snail internal organ after centrifuging and taking supernatant repurity
Biological products.
Further, the snail enzyme extract the preparation method comprises the following steps:
A. citric acid-sodium citrate buffer solution that 5-10 times of volume is added in foul in snail is homogenized, then be collected by centrifugation
Clear liquid A；
B. 80% saturation degree ammonium sulfate is added to carry out primary deposition supernatant A, after abandoning supernatant, precipitating plus PBS dissolution are obtained just
Grade concentrate B；
C. 50% saturation degree ammonium sulfate is added to by percent by volume calculating in primary concentrate B again to precipitate again, precipitate
Desalination obtains snail enzyme extract after adding PBS to dissolve.
Further, the citric acid-sodium citrate buffer solution concentration is 0.05-0.2mol/L, pH5.6-6.6.
Further, the citric acid-sodium citrate buffer solution concentration is 0.1mol/L, pH5.6-6.6.
Further, centrifugal condition is that 8000-12000rpm is centrifuged 10-15min in step a.
Further, precipitating plus 100-150 times of volume PBS dissolution in step b.
Further, precipitating plus 10-50 times of volume PBS dissolution in step c
Further, 50% saturation degree ammonium sulfate is added in step c and is precipitated as ammonium sulfate is directly added to 50% again satisfies
And degree,
Or ammonium sulfate is first added into 30% saturation degree, after centrifugation, then by supernatant add ammonium sulfate to 50% saturation
Degree is precipitated.
Further, the method for the desalination is desalting column desalination, dialysis desalting or ultrafiltration desalination.
Further, the snail enzyme extract can also be freeze-dried, spare with long-term preservation.
Further, the earth-worm extractive as raw material is the biology system of centrifuging and taking supernatant after adding buffer homogenate after earthworm is broken
Product.
Further, the earth-worm extractive as raw material can also be freeze-dried, spare with long-term preservation.
Further, the Chinese medicine composition ointment be by Chinese gall, frutus cnidii, Cortex Phellodendri, kuh-seng, wild chrysanthemum, the fruit of summer cypress,
Small fire tanning paste again after smilax and black snake add water to cook.
Further, the Chinese medicine composition ointment be by Chinese gall, frutus cnidii, Cortex Phellodendri, kuh-seng, wild chrysanthemum, the fruit of summer cypress,
Smilax and black snake add 5-10 times of water to decoct 1-2 hours, 2-3 times repeatedly, merge all decoction liquors, then small fire tanning paste is
It can.
Further, the preparation method of the ointment bases is by 120 parts of albolene, 30 parts of lanolin and atoleine
20 parts are placed in a beaker, and in 70-80 DEG C of heating water bath to melting, obtain oily phase；6 parts of additions of emulsifier sodium lauryl sulfate are steamed
170 parts of distilled water with until be completely dissolved, obtain water phase in 30 parts of mixed solutions of glycerol；By water phase heating water bath to oily phase equality of temperature
Afterwards, oil is mutually slowly added into water phase, takes out and puts to room temperature after stirring 30min under condition of water bath heating, be ointment bases.
The beneficial effects of the present invention are: the antibacterial ointment of glusulase provided by the invention contains the glusulase etc. of bacteriostatic activity
Snail extracts bioactive substance, and protein content is high, and inhibitory potency effect is more preferable.In conjunction with earth-worm extractive as raw material and Chinese traditional medicine composition
The plaster that the collocation of object is prepared can promote dyskeratosis cytothesis, and preventing and treating dry and cracked, decortication and hyperkeratosis hyperplasia causes
Skin unicorn bits.It is equal to dermatitis (nerve, anaphylaxis), psoriasis, brothers' favus of the scalp, tinea pedis and manus, tinea versicolor, eczema, nettle rash
There is certain clinical effectiveness.And the snail enzyme extract being prepared using edible snail, to human body without it is harmful at
Point.By largely using proof, non-stimulated to human skin, temporarily have no adverse reaction appearance.Preferred snail enzyme extract system
Standby preparation is simple with ointment, and stability is good, can be used for industrial production.
Detailed description of the invention
In order to keep the purpose of the present invention, technical scheme and beneficial effects clearer, the present invention provides following attached drawing and carries out
Illustrate:
Fig. 1 is that embodiment 10 uses antibacterial ointment effect contrast figure；
Fig. 2 is that embodiment 11 uses antibacterial ointment effect contrast figure；
Fig. 3 is that embodiment 12 uses antibacterial ointment effect contrast figure.
Specific embodiment
Below in conjunction with attached drawing, a preferred embodiment of the present invention will be described in detail.It is not specified in embodiment specific
The experimental method of condition, usually according to conventional conditions or according to the manufacturer's recommendations.
Embodiment 1
Selection weighs about the snail of 40-80g, is rinsed well with clear water, touches broken snail shell, and carefully peel off, will be edible
It is separated with meat and internal organ waste, wherein edible meat carries out next step food processing, uses sterile water after internal organ waste gathering
It rinses well, places -80 DEG C of refrigerator freezings as snail enzyme solution and extract raw material.The white jade snail that the present embodiment uses, agate spiral shell
Section, also referred to as Achatina, fleshy hypertrophy, full of nutrition, high protein, low fat, gallbladder are admittedly liquor-saturated to go to zero, and is rich in 20 kinds of amino
Acid.
Foul 100g in freezing snail is taken out, slightly melts, is put into tissue mashing machine's machine, add the difference of 500ml
The buffer solution of system is homogenized, and is kept under cryogenic conditions 4 DEG C after stirring extraction, and 8000-12000rpm is centrifuged 10-15min, collects
Supernatant A；Ultra-filtration centrifuge tube is reused, the sample that the buffer solution of different systems extracts all is concentrated into 50ml effect, then divide
Its protein concentration and inhibition zone size are not surveyed, and test method is as described in Example 2.
The buffer solution of the different systems of table 1 extracts result
By table 1 it is found that in the snail enzyme solution that the buffer of different buffer system extracts protein content and to Escherichia coli,
The inhibiting effect bacterium of staphylococcus aureus is variant, wherein citric acid-sodium citrate buffer solution protein content highest, concentration
After can arrive 0.715g/ml, and it is best to the inhibiting effect of Escherichia coli, staphylococcus aureus.
2 bacteriostatic experiment of embodiment
1) antibacterial strain: Escherichia coli (ATCC11229), staphylococcus aureus (ATCC6538), Candida albicans
(ATCC10231), salmonella (BZW23102), staphylococcus epidermis (ATCC12228), bacillus cereus
(ATCC63301), bacillus subtilis (ATCC6633).
2) antibacterial agent carrier: 5mm diameter circular Xinhua No.1 qualitative filter paper piece sets 120 after pressuresteam sterilization is handled
DEG C baking 2h, save backup.
3) nutrient agar: tryptose soya agar culture medium (TSA), pancreas peptone soybean broth culture medium
(TSB)。
4) bacterial suspension: by bacterial suspension preparation in Ministry of Health of the People's Republic of China " disinfection technology standard " 2.1.1.2
Program prepares the bacterial suspension of the above bacterial strain, and each bacterial suspension bacteria containing amount is respectively 5 × 105Cfu/ml~5 × 106cfu/ml。
5) inoculation of experimental bacteria: the bacterial suspension prepared is dipped with sterile cotton swab, respectively in nutrient agar
Planar surface is uniformly smeared 3 times, every to smear 1 time, and plate is rotated 60 °, is finally smeared cotton swab one week around plate edge, lid
Good plate, sets drying at room temperature 5min.
6) sterile and dry filter paper, every 5 μ l of dropwise addition sample solution, then by filter paper the preparation of bacteria inhibition tablet: are taken
It lies against in clean sterilized petri dishes, sets spare after spontaneously drying at room temperature.The preparation of negative control print: sterile dry filter is taken
The scraps of paper, every 5 μ l of dropwise addition PBS are spare after dry.
7) bacteriostatic agent print is placed with: test is placed with 1 microbiological contamination plate every time, and each plate is placed with 4 test prints, and 1
Negative control print, totally 5.It is placed with aseptic nipper coupongs in planar surface.At a distance of 25mm or more between each print center,
Periphery with plate is at a distance of 15mm or more.After being placed with, with the light pressure-like piece of aseptic nipper, it is made to be tightly attached to planar surface.It covers
Plate, sets 37 DEG C of constant incubators, and culture 16h~18h observes result.With vernier caliper (capable electronic digital indicator, 0-
150mm, ± 0.01mm) it measures the diameter (including patch) of antibacterial ring size and records.Test is repeated 5 times.When measuring antibacterial ring size, Ying Xuan
The uniformly antibacterial ring size progress of integral asepsis growth.Measuring its diameter should be with antibacterial ring size outer.The judgement of bacteriostasis: antibacterial ring size
Diameter is greater than 7mm person, has been judged to bacteriostasis；Inhibition zone diameter is less than or equal to 7mm person, is judged to no bacteriostasis；It is repeatedly same
One, which repeats test, bacteriostasis person, determines that bacteriostatic test result is effective.
Embodiment 3
Selection weighs about the snail of 40-80g, is rinsed well with clear water, touches broken snail shell, and carefully peel off, will be edible
It is separated with meat and internal organ waste, wherein edible meat carries out next step food processing, uses sterile water after internal organ waste gathering
It rinses well, places -80 DEG C of refrigerator freezings as snail enzyme solution and extract raw material.
The preparation method of snail enzyme solution:
A. foul in freezing snail is taken out, slightly melts, is put into tissue mashing machine's machine, add 5-10 times of volume (1g
It is add 5-10ml, the same below) citric acid-sodium citrate buffer solution (0.1mol/L, pH5.6-6.6) homogenate, keep cryogenic conditions
4 DEG C after lower stirring extraction, 8000-12000rpm is centrifuged 10-15min, collects supernatant A；
B. by supernatant A add 80% saturation degree ammonium sulfate carry out primary deposition (under the conditions of 4 DEG C into supernatant it is slowly few
Finely ground solid ammonium sulfate is added in amount in multiple times, excessive to avoid local salinity and lead to albuminous degeneration, and enzymatic activity reduces, with
Similarly hereinafter), after abandoning supernatant, precipitating is again plus 1:100-150 times of volume ratio PBS dissolution, PBS are cooled to 4 DEG C in advance, to guarantee enzymatic activity, obtain
Primary concentrate B；
C. it is calculated in primary concentrate B by percent by volume again plus ammonium sulfate saturation degree is 30%, 50%, 70% progress
Classification subdivision is from the precipitating respectively obtained is dissolved with 1:10-50 times of volume ratio PBS, desalination；The present embodiment experiment is de- using G-25
Salt plug carries out desalination, and dialysis, ultrafiltration or other existing desalting technologies also can be used greatly in amount；Different ammonium sulfate saturation degrees are obtained
Sample be denoted as WN30, WN50 and WN70 respectively, carry out bacterium Bactericidal test.The present embodiment has investigated ammonium sulfate precipitation respectively
With the bacteriostatic experiment of sample after the PBS dissolution of different volumes ratio, is confirmed by abundant experimental results, precipitating 1:15-30 times of body
Product is than under PBS dissolution conditions, the fungistatic effect of same sample is best, at this with 20 times of (such as 20 μ l precipitating plus 1ml PBS) PBS
Dissolution precipitating is used as same parallel testing condition experiment, the same below.Comparison:
A. foul in freezing snail is taken out, slightly melts, is put into tissue mashing machine's machine, add the lemon of 5-10 times of volume
Lemon acid-sodium citrate buffer solution (0.1mol/L, pH5.6-6.6) homogenate is kept under cryogenic conditions 4 DEG C after stirring extraction, 8000-
12000rpm is centrifuged 10-15min, collects supernatant A；
B. it is calculated in supernatant A by percent by volume again plus ammonium sulfate saturation degree is classified for 30%, 50%, 70%
It segments from the precipitating respectively obtained is dissolved with 20 times of volume PBS, desalination；The sample difference that different ammonium sulfate saturation degrees are obtained
It is denoted as DWN30, DWN50 and DWN70, carries out bacterium Bactericidal test.
Above-mentioned sample is subjected to antibacterial test respectively, test result is as shown in table 1.
1 bacteriostatic experiment test result of table
Note: test bit unit is mm in table 2, is indicated with bacteriostatic diameter, and all data are the average value of 5 experiments.
By table 2 it is found that first using 80% ammonium sulfate precipitation, a part of impurity can be first removed, is conducive to improve albumen
Matter purity and activity, so that the inhibitory potency effect of the snail enzyme solution of processing is more preferable again.Ammonium sulfate precipitation is classified again
Segment from when, the albumen that 30% and 50% ammonium sulfate obtains has good fungistatic effect, thus segment from when, can directly make
It is precipitated with 50% ammonium sulfate, can also first carry out that 30% ammonium sulfate, 50% ammonium sulfate precipitation obtains sample again.
Embodiment 4
The preparation method of snail enzyme solution:
A. foul 200g in freezing snail is taken out, slightly melts, is put into tissue mashing machine's machine, add the lemon of 1000ml
Lemon acid-sodium citrate buffer solution (0.1mol/L, pH6.6) homogenate is kept under cryogenic conditions 4 DEG C after stirring extraction, 8000-
12000rpm is centrifuged 10-15min, collects supernatant A；
B. 80% saturation degree ammonium sulfate is added to carry out primary deposition supernatant A, after abandoning supernatant, precipitating adds 1:100 times of body again
Product is dissolved than PBS, and PBS is cooled to 4 DEG C in advance, to guarantee enzymatic activity, obtains primary concentrate B；
C. it is calculated in primary concentrate B by percent by volume again plus ammonium sulfate saturation degree is classified for 30%, 50%
It segments from the precipitating respectively obtained is dissolved with 1:20 times of volume ratio PBS, desalination；The sample that different ammonium sulfate saturation degrees are obtained
It is denoted as WN30P, WN50P respectively, then is freeze-dried spare.
Embodiment 5
Earthworm living is spat net soil naturally to clean, moisture control, weighing.High-speed breakage after fast freezing, adds 1-2 times
It is homogenized under citric acid-sodium citrate buffer solution (0.1mol/L, pH6.6) low temperature of volume, high speed freezing centrifuge 8000-
12000r/min is centrifuged 15-20min, and taking supernatant is earthworm extract, then is freeze-dried spare.
Embodiment 6
By 20g Chinese gall, 10g frutus cnidii, 110g Cortex Phellodendri, 10g kuh-seng, 10g wild chrysanthemum, the 10g fruit of summer cypress, 20g smilax
It is crushed with 10g crow snake with pulverizer, adds 5-15 times of water (1-3L) to decoct 1-2 hours, 2-3 times repeatedly, merge all decoction liquors, then
Small fire tanning paste is spare, is Chinese medicine ointment.
Embodiment 7
By 25g Chinese gall, 15g frutus cnidii, 100g Cortex Phellodendri, 15g kuh-seng, 15g wild chrysanthemum, the 10g fruit of summer cypress, 20g smilax
It is crushed with 15g crow snake with pulverizer, adds 5-15 times of water to decoct 1-2 hours, 2-3 times repeatedly, merge all decoction liquors, then small fire is endured
Paste processed is spare.
Embodiment 8
By 20g Chinese gall, 15g frutus cnidii, 130g Cortex Phellodendri, 15g kuh-seng, 15g wild chrysanthemum, the 12g fruit of summer cypress, 25g smilax
It is crushed with 12g crow snake with pulverizer, adds 5-15 times of water to decoct 1-2 hours, 2-3 times repeatedly, merge all decoction liquors, then small fire is endured
Paste processed is spare.
Embodiment 9
Antibacterial ointment:
Albolene 120g, lanolin 30g and atoleine 20g are placed in a beaker, in 70-80 DEG C of heating water bath to molten
Melt, obtains oily phase；By emulsifier sodium lauryl sulfate 6g be added distilled water 170g in glycerol 30g mixed solution until complete molten
Solution, obtains water phase；By water phase heating water bath to after oily phase equality of temperature, oil is mutually slowly added into water phase, under condition of water bath heating
It takes out and is put to room temperature after stirring 30min, be ointment bases.Added in every 100g ointment bases snail enzyme solution freeze-dried powder 2-3g,
The Chinese medicine ointment 20-30g of earthworm extract freeze-dried powder 1-2g, above-described embodiment 6-8 preparation, mixing can obtain antibacterial ointment.Through
It is uniform to test the antibacterial ointment character, fine and smooth easy to apply, centrifugation and height stability are good；Matrix ductility is more preferable, is readily cleaned,
And it is suitable for surface of a wound skin；More preferable compared with water-soluble base stability, ointment medicament release is fast, penetrates, easy cleaning, and is suitable for
Surface of a wound skin.
Snail enzyme solution freeze-dried powder prepared by the present invention can also be added based on other ointment bases, earthworm extract freezes
Dry powder and above-mentioned Chinese medicine ointment prepare antibacterial ointment.
By antibacterial ointment prepared by the present invention to dermatitis (nerve, anaphylaxis), psoriasis, brothers' favus of the scalp, tinea pedis and manus, flower
Spot tinea, eczema, nettle rash have certain clinical effectiveness.Ointment has safe and non-toxic, easy to use, curative for effect and patient
The features such as compliance is good can improve the various inconvenience of traditional Chinese medicine therapy bring, Chinese medicine is made preferably to play a role.
Embodiment 10
35 years old male's whole bodies suffer from psoriasis for many years, seek doctor everywhere and have no result, and daily itch is hard to bear, continuous present invention preparation suppression
Bacterium ointment 21 days, itch degree is gradually reduced, slight place starts to form a scab；Continue to smear 1 month, it is most of recovered good
Turn, as shown in Fig. 1 comparison, wherein a is to start symptom, and b is after improving.
Embodiment 11
Hu 48 years old, is corroded by cement throughout the year, with palm blister, furfur, it is coarse thicken, dry-cracking, consciously itch pain
For the tinea class skin disease mainly showed, antibacterial ointment is prepared 16 days using the present invention, has been peeled, it is bright after continuing to use 1 month
It is aobvious to form a scab and have neoplastic skin appearance.As shown in Fig. 2 comparison, wherein a is to start symptom, and b is after improving.
Embodiment 12
Mr. Li, 38 years old, female, because both hands soak for a long time and contact detergent, the tinea manuum was as long as half a year, through other drugs
Treatment, take a favorable turn, but after a period of time and does not stop to recur.Antibacterial ointment is prepared 10 days using the present invention, itch disappears, followed by
After 7 days, all symptoms completely disappear continuous smearing.As shown in Fig. 3 comparison, wherein a is and to be coated with soft using effect after 10 days
Cream, b are that symptom completely disappears rear effect.
Finally, it is stated that preferred embodiment above is only used to illustrate the technical scheme of the present invention and not to limit it, although logical
It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (10)
1. a kind of antibacterial ointment of glusulase, which is characterized in that the raw material of the antibacterial ointment of glusulase includes ointment bases, snail
Enzyme extract, earth-worm extractive as raw material and Chinese medicine composition ointment；The raw material of the Chinese medicine composition ointment be Chinese gall, frutus cnidii,
Cortex Phellodendri, kuh-seng, wild chrysanthemum, the fruit of summer cypress, smilax and black snake.
2. the antibacterial ointment of glusulase according to claim 1, which is characterized in that according to the mass fraction, the glusulase suppression
In the raw material of bacterium ointment, 100 parts of ointment bases, 2-3 parts of snail enzyme extract, 1-2 parts of earth-worm extractive as raw material, Chinese medicine composition ointment
20-30 parts.
3. the antibacterial ointment of glusulase according to claim 1, which is characterized in that according to the mass fraction, Chinese medicine composition medicine
In the raw material of cream: 20-25 portions of Chinese galls, 10-15 parts of frutus cnidiis, 100-130 portions of Cortex Phellodendris, 10-15 parts of kuh-sengs, 10-15 portions of mother chrysanthemums
The black snake of flower, the 10-12 parts of fruit of summer cypress, 20-25 portions of smilaxs and 10-15 parts.
4. the antibacterial ointment of glusulase according to claim 1-3, which is characterized in that the snail enzyme extract is
Biological products after snail internal organ add buffer to be homogenized after centrifuging and taking supernatant repurity.
5. the antibacterial ointment of glusulase according to claim 4, which is characterized in that the preparation method of the snail enzyme extract
Are as follows:
A. citric acid-sodium citrate buffer solution that 5-10 times of volume is added in foul in snail is homogenized, then supernatant is collected by centrifugation
A；
B. 80% saturation degree ammonium sulfate is added to carry out primary deposition supernatant A, after abandoning supernatant, precipitating plus PBS dissolution are obtained primary dense
Contracting liquid B；
C. it adds to 50% saturation degree ammonium sulfate by percent by volume calculating in primary concentrate B again to precipitate again, precipitating plus PBS
Desalination obtains snail enzyme extract after dissolution.
6. the antibacterial ointment of glusulase according to claim 5, which is characterized in that add to 50% saturation degree sulfuric acid in step c
Ammonium is precipitated as that ammonium sulfate is directly added to 50% saturation degree again,
Or ammonium sulfate is first added into 30% saturation degree, after centrifugation, then by supernatant add ammonium sulfate to 50% saturation degree into
Row precipitating.
7. the antibacterial ointment of glusulase according to claim 4, which is characterized in that the earth-worm extractive as raw material is after earthworm is broken
The biological products of centrifuging and taking supernatant after adding buffer to be homogenized.
8. the antibacterial ointment of glusulase according to claim 4, which is characterized in that the Chinese medicine composition ointment is by five times
Small fire tanning paste again after son, frutus cnidii, Cortex Phellodendri, kuh-seng, wild chrysanthemum, the fruit of summer cypress, smilax and black snake add water to cook.
9. the antibacterial ointment of glusulase according to claim 8, which is characterized in that the Chinese medicine composition ointment is by five times
Son, frutus cnidii, Cortex Phellodendri, kuh-seng, wild chrysanthemum, the fruit of summer cypress, smilax and black snake add 5-10 times of water decoction 1-2 hours, repeatedly 2-3
It is secondary, merge all decoction liquors, then small fire boils paste.
10. the antibacterial ointment of glusulase according to claim 4, which is characterized in that the preparation method of the ointment bases is
120 parts of albolene, 30 parts of lanolin and 20 parts of atoleine are placed in a beaker, in 70-80 DEG C of heating water bath to melting, are obtained
Oily phase；By 6 parts of emulsifier sodium lauryl sulfate, 170 parts of addition distilled water in 30 parts of mixed solutions of glycerol until completely it is molten
Solution, obtains water phase；By water phase heating water bath to after oily phase equality of temperature, oil is mutually slowly added into water phase, under condition of water bath heating
It takes out and is put to room temperature after stirring 30min, be ointment bases.
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| CN110559428A (en) * | 2019-10-22 | 2019-12-13 | 福建德康宝生物科技有限公司 | Snail enzyme mixed ointment for treating children eczema and preparation method thereof |
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Application publication date: 20190827 |