CN110559429A

CN110559429A - Snail enzyme rhinitis ointment and preparation method thereof

Info

Publication number: CN110559429A
Application number: CN201911006561.1A
Authority: CN
Inventors: 刘侠; 杨范斌
Original assignee: Fujian Dekangbao Biotechnology Co Ltd
Current assignee: Fujian Dekangbao Biotechnology Co Ltd
Priority date: 2019-10-22
Filing date: 2019-10-22
Publication date: 2019-12-13
Anticipated expiration: 2039-10-22
Also published as: CN110559429B

Abstract

a rhinitis ointment containing helicase and its preparation method are provided, wherein the raw materials of the said helicase rhinitis ointment include helicase, lumbrokinase and Chinese medicinal composition ointment; the raw materials of the traditional Chinese medicine composition ointment comprise flos magnoliae, radix angelicae, centipeda minima, propolis, wild chrysanthemum, mint, lanolin, white muscardine silkworm and broadleaf holly leaf, and the traditional Chinese medicine composition ointment is simple in formula combination and free of toxic and side effects of chemical additives.

Description

Snail enzyme rhinitis ointment and preparation method thereof

Technical Field

The invention belongs to the technical field of biological products, and relates to helicase rhinitis cream and a preparation method thereof.

Background

Rhinitis, an inflammatory disease of the nasal cavity, is inflammation of the mucous membrane of the nasal cavity caused by viruses, bacteria, allergens, various physicochemical factors and certain systemic diseases. The main pathological changes of rhinitis are congestion, swelling, exudation, hyperplasia, atrophy or necrosis of the nasal mucosa.

When rhinitis occurs, the normal work and life of people can be seriously affected by symptoms such as nasal obstruction, continuous sneezing, nasal discharge, dizziness, headache and the like. Because the cause of rhinitis is complicated, when a patient uses some western medicines for treatment, the symptoms are treated, the root causes are not treated, and the treatment effect is not ideal. Moreover, some western medicines such as 0.5% -1% ephedrine nasal drops used for a long time at present can damage nasal mucociliary structures and aggravate rhinitis more easily. Although the western medicines can effectively control the symptoms of rhinitis, the rhinitis is difficult to completely cure, frequently recurs and has serious toxic and side effects.

The urban industrialization process aggravates the environmental deterioration, rhinitis patients in China reach 3 hundred million, the disease rate reaches 37 percent, the number of the rhinitis patients is increased at a speed of 3 percent each year, teenager and children rhinitis patients account for 2/3 of rhinitis groups, and the number of the rhinitis patients is gradually increased at a speed of 3 percent each year (2000-3000 ten thousand).

Disclosure of Invention

Aiming at the problems, the invention provides the helicase rhinitis cream which is simple in formula combination and free of toxic and side effects of chemical additives and the preparation method thereof, so as to overcome the problems in the prior related art.

In order to realize the purpose of the invention, the invention adopts the following technical scheme:

A rhinitis ointment containing helicase is prepared from helicase, lumbrokinase and Chinese medicinal composition ointment; the Chinese medicinal composition ointment is prepared from flos Magnoliae, radix Angelicae Dahuricae, herba Centipedae, propolis, flos Chrysanthemi Indici, herba Menthae, lanolin, Bombyx Batryticatus, and Folum Ilicis.

Furthermore, the raw materials of the helicase rhinitis cream comprise, by mass, 10-20 parts of helicase and 10-20 parts of lead kinase; 3-8 parts of magnolia flower, 3-8 parts of angelica dahurica, 5-15 parts of centipeda minima, 2-5 parts of propolis, 2-5 parts of wild chrysanthemum flower, 0.5-1.5 parts of mint, 23-28 parts of lanolin, 3-8 parts of white muscardine silkworm and 3-8 parts of broadleaf holly leaf.

Further, by mass, 15 parts of snailase, 15 parts of lumbrokinase, 5 parts of flos magnoliae, 5 parts of radix angelicae, 10 parts of centipeda minima, 3 parts of propolis, 3 parts of wild chrysanthemum flower, 1 part of mint, 25 parts of lanolin, 5 parts of white muscardine silkworm and 5 parts of broadleaf holly leaf.

A preparation method for preparing the helicase rhinitis cream comprises the following specific steps:

(1) Weighing all raw materials required by the helicase rhinitis cream according to the mass parts;

(2) Cleaning flos Magnoliae, radix Angelicae Dahuricae, herba Centipedae, propolis, flos Chrysanthemi Indici, herba Menthae, Bombyx Batryticatus, and Folum Ilicis, and air drying;

(3) Respectively grinding flos Magnoliae, radix Angelicae Dahuricae, herba Centipedae, propolis, flos Chrysanthemi Indici, herba Menthae, Bombyx Batryticatus, and Folum Ilicis into medicinal powder, and stirring to obtain mixed medicinal powder I;

(4) Soaking the first mixed powder in water for 12-24h, heating and decocting for 2-4h, and filtering to obtain a first filtrate;

(5) Mixing the filtrate I with lanolin, heating, and concentrating to obtain paste I;

(6) After the paste I is cooled, adding the helicase and the lumbrokinase, and fully and uniformly stirring to obtain a paste II;

(7) And sealing the second paste in vacuum, and fermenting for 20-30 days to obtain the helicase rhinitis ointment.

further, the granularity of the medicinal powder in the step (3) is 300 meshes.

further, the heating temperature in the step (4) was 100 ℃.

Further, the heating temperature in the step (5) is 90-100 ℃, and the heating time is 30-60 min.

Further, the stirring time in the step (6) is 2 to 4 hours.

Furthermore, the preparation containers used in the preparation process of the helicase rhinitis cream are all made of tile containers, so that the medicine can be fused more fully and the reaction is more thorough.

The snailase rhinitis cream provided by the invention is prepared by taking flos magnoliae, radix angelicae, centipeda minima, propolis, wild chrysanthemum, mint, white muscardine silkworm and broadleaf holly leaf as raw materials, crushing, weighing in proportion, and uniformly stirring, wherein the flos magnoliae (which is pungent in flavor and warm in property and good in relieving stuffy nose) and the centipeda minima (which is pungent in flavor and warm in property and good in relieving stuffy nose) in the formula can be used for treating nasosinusitis; radix Angelicae Dahuricae, flos Chrysanthemi Indici, herba Menthae, Bombyx Batryticatus, and Folum Ilicis have effects of dispelling pathogenic wind and cold, dispelling pathogenic wind and heat, and clearing lung heat, and can be used for treating rhinitis, headache, nasal obstruction, and watery nasal discharge. Lanolin is a very good moisturizing product as a natural ointment base, and can keep the skin moist when being applied to the skin, so that the skin tissue of the wound part is kept in an optimal state. The snail enzyme is used as a catalyst, the drug effect of the important combination is fully exerted, and the lumbrukinase is used as a drug guide to ensure that the drug can rapidly enter damaged cells, thereby accelerating the repair capability of the cells. The application of the rhinitis ointment prevents bacteria from contacting with skin in the air, thereby reducing the possibility of infection and accelerating the repair speed of damaged tissues.

The raw materials of the helicase rhinitis cream are all pure natural substance formulas, the reaction period is further shortened and the drug effect is enhanced by the corresponding physicochemical reaction generated after the important materials in the formulas are fully fused and the catalytic action of the helicase is added, the pure biology is safe and has no toxic or side effect, the harm to children and the old is avoided, and the helicase rhinitis cream can be used by any person and has good antibacterial and repairing effects. By combining advanced biotechnology with traditional Chinese medicine, the traditional Chinese medicine plays a greater role.

Description of the drawings:

Is free of

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely a few embodiments of the invention and are not to be taken as the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.

a rhinitis ointment containing helicase is prepared from helicase, lumbrokinase and Chinese medicinal composition ointment; the Chinese medicinal composition ointment is prepared from flos Magnoliae, radix Angelicae Dahuricae, herba Centipedae, propolis, flos Chrysanthemi Indici, herba Menthae, lanolin, Bombyx Batryticatus, and Folum Ilicis. The snail enzyme rhinitis cream comprises, by mass, 10-20 parts of snail enzyme and 10-20 parts of lead kinase. The traditional Chinese medicine composition ointment comprises the following components in parts by weight: 3-8 parts of magnolia flower, 3-8 parts of angelica dahurica, 5-15 parts of centipeda minima, 2-5 parts of propolis, 2-5 parts of wild chrysanthemum flower, 0.5-1.5 parts of mint, 23-28 parts of lanolin, 3-8 parts of white muscardine silkworm and 3-8 parts of broadleaf holly leaf.

The invention is further described in detail below in the context of several specific sets of examples: in the following example 1 part is 1 g.

1. Example 1

Snail enzyme rhinitis ointment comprises the following raw materials in parts by mass:

And 3 parts of magnolia flower with the particle size of 200 meshes.

2 parts of wild chrysanthemum flower with the particle size of 200 meshes.

0.5 part of mint with the particle size of 200 meshes.

And 3 parts of broadleaf holly leaf with the particle size of 200 meshes.

And 3 parts of angelica dahurica with the particle size of 200 meshes.

5 portions of centipeda minima with the grain diameter of 200 meshes.

3 parts of white muscardine silkworm with the particle size of 200 meshes.

2 parts of propolis.

And 23 parts of lanolin.

10 portions of helicase with the grain diameter of 2000 meshes.

10 parts of lumbrokinase with the grain diameter of 2000 meshes.

The preparation method of the helicase rhinitis cream provided by the embodiment comprises the following specific steps:

(1) Cleaning flos Magnoliae, radix Angelicae Dahuricae, herba Centipedae, propolis, flos Chrysanthemi Indici, herba Menthae, Bombyx Batryticatus, and folium Ilicis, and air drying;

(2) Respectively grinding flos Magnoliae, radix Angelicae Dahuricae, herba Centipedae, propolis, flos Chrysanthemi Indici, herba Menthae, Bombyx Batryticatus, and Folum Ilicis into powder (particle size of 200 mesh), and stirring to obtain mixed powder I.

(3) Soaking the first mixed powder in water for 12h, heating (100 deg.C), decocting (2h), and filtering to obtain the first filtrate.

(4) Mixing the first filtrate with lanolin, heating for 90-30 min, and concentrating to obtain a first paste.

(5) and (3) after the paste I is cooled, adding helicase (2000 meshes) and lumbrokinase (2000 meshes) and fully and uniformly stirring (stirring for 2h) to obtain a paste II.

(6) and sealing the second paste in vacuum, and fermenting for 20 days to obtain the helicase rhinitis ointment.

Example 2

and 5 parts of magnolia flower with the particle size of 400 meshes.

3 parts of wild chrysanthemum flower with the particle size of 400 meshes.

1 part of mint with the particle size of 400 meshes.

5 parts of broadleaf holly leaf with the particle size of 400 meshes.

5 portions of angelica dahurica with the grain diameter of 400 meshes.

8 portions of centipeda minima with the grain diameter of 400 meshes.

5 portions of white muscardine silkworm with the grain diameter of 400 meshes.

4 parts of propolis.

25 parts of lanolin.

15 portions of helicase with the grain size of 3000 meshes.

15 parts of lumbrokinase with the grain diameter of 3000 meshes.

(2) Respectively grinding flos Magnoliae, radix Angelicae Dahuricae, herba Centipedae, propolis, flos Chrysanthemi Indici, herba Menthae, Bombyx Batryticatus, and Folum Ilicis into powder (particle size of 400 mesh), and stirring to obtain mixed powder I.

(3) Soaking the first mixed powder in water for 18h, heating (100 deg.C), decocting (3h), and filtering to obtain filtrate I.

(4) Mixing the first filtrate with lanolin, heating for 95-45 min, and concentrating to obtain a first paste.

(5) And after the paste I is cooled, adding helicase (3000 meshes) and lumbrokinase (3000 meshes) and fully stirring (stirring for 3 hours) uniformly to obtain a paste II.

(6) And sealing the second paste in vacuum, and fermenting for 25 days to obtain the helicase rhinitis ointment.

example 3

8 parts of magnolia flower with the particle size of 200 meshes.

5 parts of wild chrysanthemum flower with the particle size of 200 meshes.

1.5 parts of mint with the particle size of 200 meshes.

8 parts of broadleaf holly leaf with the particle size of 200 meshes.

8 portions of angelica dahurica with the grain diameter of 200 meshes.

15 portions of centipeda minima with the grain diameter of 200 meshes.

8 portions of white muscardine silkworm with the grain diameter of 200 meshes.

5 parts of propolis.

28 parts of lanolin.

20 portions of helicase with the grain size of 3000 meshes.

20 parts of lumbrokinase with the grain diameter of 3000 meshes.

(3) Soaking the first mixed powder in water for 18h, heating (100 deg.C), decocting (4h), and filtering to obtain filtrate I.

(4) Mixing the first filtrate with lanolin, heating for 100-60 min, and concentrating to obtain a first paste.

(5) and after the paste I is cooled, adding helicase (3000 meshes) and lumbrokinase (3000 meshes) and fully stirring (stirring for 4h) uniformly to obtain a paste II.

(6) And sealing the second paste in vacuum, and fermenting for 30 days to obtain the helicase rhinitis ointment.

Example 4

snail enzyme rhinitis ointment comprises the following raw materials in parts by weight:

10 parts of magnolia flower with the grain diameter of 400 meshes.

8 parts of wild chrysanthemum flower with the particle size of 400 meshes.

2 portions of mint with the grain diameter of 400 meshes.

10 portions of broadleaf holly leaf with the grain diameter of 400 meshes.

8 portions of angelica dahurica with the grain diameter of 400 meshes.

10 portions of centipeda minima with the grain diameter of 400 meshes.

8 portions of white muscardine silkworm with the grain diameter of 400 meshes.

6 parts of propolis.

And 30 parts of lanolin.

25 portions of helicase with the grain diameter of 2000 meshes.

25 parts of lumbrokinase with the particle size of 2000 meshes.

(4) Mixing the above filtrate with lanolin, heating (95-60 min), and concentrating to obtain paste I.

(5) And after the paste I is cooled, adding helicase (2000 meshes) and lumbrokinase (2000 meshes) and fully stirring (stirring for 2h) uniformly to obtain a paste II.

(6) And (5) sealing and storing the second paste in vacuum for 28 days to obtain the helicase rhinitis ointment.

Examples of the experiments

The inventor carries out follow-up tests on people (50 children and 50 adults) using the helicase rhinitis cream. The snail enzyme rhinitis ointment has obvious curative effect and low recurrence rate, and especially has faster and better effect for children.

Xuan, male, 33 years old, suffered from allergic rhinitis for 10 years, and continuously sneezed and rhinorrhea in and out of an air conditioner, after 10 g of the snailase rhinitis ointment of the invention is used, the ointment can be cured, and no relapse occurs in 2 years.

Old, female, 15 years old. The chronic rhinitis is suffered for 2 years, and the learning is seriously influenced by frequent nasal obstruction and nasal discharge. After 5 g of the snail enzyme rhinitis ointment is used, the rhinitis ointment can be healed, and no relapse occurs in 3 years.

Liu somean, female 38 years old, nasal concha hypertrophy for 6 years, nasal obstruction, nasal cavity muscle swelling, pale nasal cavity mucosa and hyposmia, after 10 g of the snail enzyme rhinitis cream is used for curing, no relapse occurs in 1 year.

In order to facilitate understanding of the present invention, the following further description of the raw materials and the pharmaceutical effects of the present invention is provided:

Snails belong to the phylum mollusca, class gastropoda, and many types of parts are edible (e.g. french snails, agate snails, etc.). The feed has wide sources, strong reproductive capacity and simple feeding, mainly uses plant materials rich in cellulose in communities, and can take glucose generated by hydrolysis of the cellulose as an energy substance for growth and development. The snail meat has high protein, low fat, no cholesterol, delicate meat quality and delicious taste, and also has high medicinal value. Snailase (snail extract): snailase is a mixed enzyme prepared from the bursa and digestive tract of Snail, and contains more than 20 enzymes (cellulase, pectinase, amylase, etc.) such as cellulase, pectinase, amylase, etc. Helicase is an enzyme of great value. It can be used for the disruption of yeast cell walls, and thus is widely used for the research of cell biology and genetic engineering; it can be used as feed additive to improve the digestibility of feed; it can also be used for clarifying fruit juice, removing capsule coat of fructus Citri Tangerinae, and making jam. In the helicase rhinitis cream, the helicase plays a role of reacting various traditional Chinese medicines in the traditional Chinese medicine composition to exert the drug effect to the maximum extent in a very short time through the catalytic action of the helicase which is a compound enzyme.

flos Magnoliae, named as Chinese medicine. Also called magnolia, magnolia liliiflora, is a unique plant in China, and is a traditional flower and traditional Chinese medicine with more than 2000 years of history in China. Mild in nature and pungent in flavor, and mainly used for dispersing wind-cold and relieving stuffy nose.

The angelica dahurica, as a root medicine, has the functions of dispelling diseases, removing dampness, expelling pus, promoting granulation, promoting blood circulation, relieving pain and the like. Can be used for treating wind-cold type common cold, headache, rhinitis, and toothache. It can also be used as perfume for treating leucorrhea with red and white discharge, pain, furuncle, and toxic swelling.

Centipeda minima, centipeda minima and the name of traditional Chinese medicine. Is the whole plant of Centipeda minima (L.) A.Braun e tAschers. Has effects of dispelling pathogenic wind and cold, relieving nasal obstruction, relieving cough, and removing toxic substance. It can be used for treating wind-cold type common cold, nasal obstruction, cough with asthma due to cold-phlegm, sore, carbuncle, and toxic swelling.

Propolis, a traditional natural medicine, has been used in europe for over 2000 years. Propolis is currently recorded in the Chinese pharmacopoeia. Like other bee products, propolis has a wide range of pharmacological activities, such as antibacterial, antiviral, antipathogenic, antioxidant, anticancer, anesthetic, wound repair, anti-inflammatory, immunomodulating, caries prevention, cardiovascular protection, etc. Propolis has multiple pharmacological activities, and is widely applied to the fields of medicines, health-care foods, cosmetics and the like.

Wild chrysanthemum flower, leaf, flower and whole herb of wild chrysanthemum flower. Bitter, pungent and cool in taste, has the effects of clearing away heat and toxic materials, dispelling pathogenic wind and heat, removing blood stasis, improving eyesight, and lowering blood pressure. It can be used for preventing and treating epidemic cerebrospinal meningitis, influenza, hypertension, hepatitis, dysentery, carbuncle, furuncle, and sore. The infusion of wild chrysanthemum is also very effective for killing wigglers and fly maggots.

Mint, name of traditional Chinese medicine. The soil is named 'Yindancao', is a labiate plant, namely other dry whole plants of the same genus, mostly grows beside a mountain wetland river, and roots and stems transversely grow underground, and is an aromatic crop with special economic value. It is a sweating and antipyretic, and can be used for treating influenza, headache, conjunctival congestion, fever, sore throat, and gum swelling. The mint picked in the bright greenhouse is also a fresh vegetable on a dining table in spring festival, and is fresh, cool and delicious. Herba Menthae is usually used instead of tea for clearing away heart-fire and improving eyesight.

Lanolin, a secreted oil attached to wool, is an ester of sterols, fatty alcohols and triterpene alcohols with approximately equal amounts of fatty acids as the main component. Lanolin can make skin smooth and tender. The skin beautifying effect of the lanolin fatty acid is discovered at the earliest because the hands of workers who raise Australian sheep are generally tender than those of ordinary people, and the lanolin fatty acid is discovered through research. The product is used for preparing high-grade antirust oil, low-temperature lubricant, printing ink, fiber oil, leather fatting agent, plastic plasticizer, latex defoamer and the like in industry. It can be used for preparing rheumatism plaster, zinc oxide plaster and ointment base material. Cosmetic grade lanolin can be used in cold cream, wrinkle-preventing cream, anti-chap cream, shampoo, hair conditioner, hair cream, lipstick, high-grade soap, etc. Is commonly used as a water-in-oil emulsifier and is an excellent moisturizing substance. The dry or rough skin due to lack of natural moisture can be softened and recovered. It maintains the normal moisture content of the skin by delaying, rather than completely preventing, the passage of moisture through the epidermal layers.

Bombyx Batryticatus, alternative name: bombyx Batryticatus and Bombyx Batryticatus, neutral in nature, pungent and salty in flavor. The dry silkworm body is a kind of wind-extinguishing and spasm-stopping medicine, and has the main functions of stopping wind and spasm, dispelling wind and stopping pain, eliminating phlegm and dissipating mass, and may be used in treating convulsion, facial distortion, wind-heat headache, conjunctival congestion, pharyngalgia, rubella, etc. in 5-10 g. Is widely used for diseases such as allergic rhinitis and the like.

Kuding tea is cold in nature and bitter in taste. The main effects are as follows: dispelling wind and clearing heat; improving eyesight and promoting fluid production. Headache due to wind-heat; dental pain; conjunctival congestion; purulent otitis media; aphtha; fever with polydipsia; diarrhea; dysentery is caused.

Lumbrukinase is a group of proteolytic enzymes (acid proteins) extracted from special earthworms, and has a molecular weight of 1.6-4.5 ten thousand D. Lumbrokinase is an antithrombotic drug and is mainly used for thrombotic diseases clinically, in particular for patients with increased fibrinogen and platelet aggregation rate; can be used for treating ischemic cardiovascular and cerebrovascular diseases, and improving symptoms and preventing disease development. In the snail enzyme rhinitis ointment, lumbrukinase plays a role as a guiding drug, and the medicine is brought into nasal cavity tissues by using the penetrability of cardiovascular diseases of the lumbrukinase.

while the foregoing description shows and describes the preferred embodiments of the present invention, it is to be understood that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as described herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. A rhinitis ointment containing helicase is characterized in that the raw materials of the helicase rhinitis ointment comprise helicase, lumbrokinase and a Chinese medicinal composition ointment; the Chinese medicinal composition ointment is prepared from flos Magnoliae, radix Angelicae Dahuricae, herba Centipedae, propolis, flos Chrysanthemi Indici, herba Menthae, lanolin, Bombyx Batryticatus, and Folum Ilicis.

2. The rhinitis cream containing the helicase as claimed in claim 1, which is characterized in that the raw materials of the helicase rhinitis cream comprise, by mass, 10-20 parts of the helicase and 10-20 parts of lumbrokinase; 3-8 parts of magnolia flower, 3-8 parts of angelica dahurica, 5-15 parts of centipeda minima, 2-5 parts of propolis, 2-5 parts of wild chrysanthemum flower, 0.5-1.5 parts of mint, 23-28 parts of lanolin, 3-8 parts of white muscardine silkworm and 3-8 parts of broadleaf holly leaf.

3. The rhinitis cream containing helicase as claimed in claim 2, which is characterized by comprising, by mass, 15 parts of helicase, 15 parts of lumbrokinase, 5 parts of flos magnoliae, 5 parts of radix angelicae, 10 parts of centipeda minima, 3 parts of propolis, 3 parts of wild chrysanthemum flower, 1 part of mint, 25 parts of lanolin, 5 parts of white muscardine silkworm and 5 parts of broadleaf holly leaf.

4. A process for the preparation of a helicase rhinitis cream as claimed in claim 1, 2 or 3, characterised in that: the method comprises the following specific steps:

5. A method of preparing a snailase rhinitis cream as claimed in claim 4, wherein: the granularity of the medicinal powder in the step (3) is 300 meshes.

6. A method of preparing a snailase rhinitis cream as claimed in claim 4, wherein: the heating temperature in the step (4) was 100 ℃.

7. A method of preparing a snailase rhinitis cream as claimed in claim 4, wherein: the heating temperature in the step (5) is 90-100 ℃, and the heating time is 30-60 min.

8. a method of preparing a snailase rhinitis cream as claimed in claim 4, wherein: the stirring time in the step (6) is 2-4 hours.

9. A method of preparing a snailase rhinitis cream as claimed in claim 4, wherein: the preparation containers used in the preparation process of the helicase rhinitis cream are all made of tile containers, so that the medicine can be fused more fully and the reaction is more thorough.