CN105685939A - Hippophae rhamnoides soybean sauce and production method thereof - Google Patents

Hippophae rhamnoides soybean sauce and production method thereof Download PDF

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Publication number
CN105685939A
CN105685939A CN201610097340.XA CN201610097340A CN105685939A CN 105685939 A CN105685939 A CN 105685939A CN 201610097340 A CN201610097340 A CN 201610097340A CN 105685939 A CN105685939 A CN 105685939A
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CN
China
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parts
hippophae
fructus
hippophae rhamnoides
herba
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CN201610097340.XA
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Chinese (zh)
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陈爱华
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Individual
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Individual
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Priority to CN201610097340.XA priority Critical patent/CN105685939A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Medicines Containing Plant Substances (AREA)

Abstract

The invention provides hippophae rhamnoides soybean sauce. The hippophae rhamnoides soybean sauce is produced by, by weight, 18 parts of fructus hippophae, 20 parts of spatholirion longifolium, 18 parts of soroseris erysimoides, 10 parts of fructus phyllanthi, 12 parts of houttuynia cordata, 200 parts of Haitian dark soybean sauce, 50 parts of honey and 50 white sugar. The hippophae rhamnoides soybean sauce is excellent in sterilizing effect.

Description

A kind of hippophae rhamnoides soy and preparation method thereof
Technical field
The present invention relates to a kind of hippophae rhamnoides soy and preparation method thereof, belong to food technology field。
Background technology
Fructus Hippophae is a kind of natural plants of application newly developed in recent years, and it is natural nutrition good merchantable brand, has again special health-care effect, is also the fine tree species preserved the ecological environment。Fructus Hippophae contains nearly 200 kinds and has nutritive value and bioactive material, be eat, medicinal, beauty treatment and the former section of ideal of animal feed。
Summary of the invention
Goal of the invention: it is an object of the invention to provide a kind of hippophae rhamnoides soy and preparation method thereof。
Technical scheme: it is an object of the invention to by following scheme realization:
A kind of hippophae rhamnoides soy, it is characterised in that be made up of the raw material of following weight portion: Fructus Hippophae 18 parts, Folium Bambusae Herba Reineckeae Carneae 20 parts, Herba Crepidis hookerianae 18 parts, Fructus Phyllanthi 10 parts, Herba Houttuyniae 12 parts, sky, sea dark soy sauce 200 parts, Mel 50 parts, white sugar 50 parts。
Described hippophae rhamnoides soy, preparation method comprises the following steps: take Fructus Hippophae, Folium Longan, Fructus Alpinia Japanicae, Caulis et Folium Hyptidis suaveolentis fruit, Herba Lophatheri, add the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, medicinal liquid leaches, merge twice decoction liquor, being evaporated to 60 DEG C of relative densities is 1.05, adding ethanol makes the percent by volume of alcohol content reach 75%, stirring, stand, filter, when filtrate reduced in volume is to 65 DEG C, relative density is 1.25, and reclaim ethanol, obtain concentrated solution, again with sky, sea dark soy sauce, Mel and white sugar mixing, it is sufficiently stirred for and makes white sugar dissolve, mixing。
Folium Bambusae Herba Reineckeae Carneae is Commelianaceae, Folium Bambusae Herba Reineckeae Carneae platymiscium Folium Bambusae Herba Reineckeae Carneae Spatholirionlongifolium (Gagnep.) Dunn, is used as medicine with inflorescence。Summer gathers, and dries。Regulating menstruation, pain relieving。For menoxenia, nervous headache。The present invention is with all herbal medicine。
Herba Crepidis hookerianae, Classification system is Soroserishookeriana (C.B.Clarke) Stebb.ssp.erysimoides (Hand.-Mazz.) Stebb, Compositae, and Herba Soroseridis erysimoidis belongs to, nourishing the lung to arrest cough, regulating menstruation, hemostasis, antiinflammatory, stimulating milk secretion。For cold cough, bronchitis, agalactia, mastitis, menoxenia, red collapse, leucorrhea, epistaxis, furuncle carbuncle。The present invention is with all herbal medicine。
Fructus Phyllanthi is the dry mature fruit of Euphorbiaceae phyllanthus emblica platymiscium Fructus Phyllanthi PhyllanthusemblicaL.。Gather when winter is to secondary spring fruit maturation, remove impurity, dry。Clearing away heat and cooling blood, promoting digestion and invigorating the stomach, cough-relieving of promoting the production of body fluid。For heat in blood blood stasis, liver-gallbladder disease, dyspepsia, stomachache, cough, laryngalgia, xerostomia。All the other are pharmacopeia kind。
Beneficial effect: the present invention can disinfection, the feature of environmental protection is good, and result of use is good and convenient。
Detailed description of the invention
Form by the following examples, the foregoing of the present invention is described in further detail again, but this should not being interpreted as, the scope of the above-mentioned theme of the present invention is only limitted to Examples below, and all technology realized based on foregoing of the present invention belong to the scope of the present invention。
Embodiment 1: weigh Fructus Hippophae 18g, Folium Bambusae Herba Reineckeae Carneae 20g, Herba Crepidis hookerianae 18g, Fructus Phyllanthi 10g, Herba Houttuyniae 12g, sea sky dark soy sauce 200g, Mel 50g, white sugar 50g, take Fructus Hippophae, Folium Longan, Fructus Alpinia Japanicae, Caulis et Folium Hyptidis suaveolentis fruit, Herba Lophatheri, add the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, medicinal liquid leaches, merge twice decoction liquor, being evaporated to 60 DEG C of relative densities is 1.05, adding ethanol makes the percent by volume of alcohol content reach 75%, stirring, stand, filter, when filtrate reduced in volume is to 65 DEG C, relative density is 1.25, and reclaim ethanol, obtain concentrated solution, again with sky, sea dark soy sauce, Mel and white sugar mixing, it is sufficiently stirred for and makes white sugar dissolve, mixing。
Embodiment 2: bacteriostatic test of the present invention
Medicine and reagent: the present invention is prepared by embodiment 1 preparation method, matched group preparation method: weigh Fructus Hippophae 18g, sea sky dark soy sauce 200g, Mel 50g, white sugar 50g, take Fructus Hippophae, add the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, medicinal liquid leaches, merge twice decoction liquor, being evaporated to 60 DEG C of relative densities is 1.05, adding ethanol makes the percent by volume of alcohol content reach 75%, stirring, stand, filter, when filtrate reduced in volume is to 65 DEG C, relative density is 1.25, and reclaim ethanol, obtain concentrated solution, again with sky, sea dark soy sauce, Mel and white sugar mixing, it is sufficiently stirred for and makes white sugar dissolve, mixing。, it is configured to matched group hippophae rhamnoides soy;Penicillin sodium: Huabei Pharmaceutic Co., Ltd's specification: 800,000 units/bottle lot number: Y1205154, sodium chloride, Shanghai hundred million chemical reagent company limiteies of a specified duration, lot number: 00090130。Nutrient broth, CAS Shanghai Insect Bio Technological Development Co., Ltd., lot number: 130233。Nutrient agar, microorganism reagent factory of Jiangsu Prov. Disease Preventing and Controlling Center, lot number: 140026。Calf serum, Hangzhou Sijiqing Biological Engineering Material Co., Ltd., lot number: 140405;De-fine Sheep Blood, purchased from Sheep Blood supply station, Nanjing, lot number: 140803
Bacterial strain: Clinical isolation: staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, Candida albicans, is provided by Jiangsu Prov. People's Hospital microbial room isolation identification。
Instrument and equipment: electronic balance, Beijing Sai Duolisi balance company limited produces;Full-automatic high-pressure autoclave, Japan SANYO;Superclean bench, Suzhou Decontamination Equipment Plant;Water isolation type constant incubator, the permanent Science and Technology Ltd. in Shanghai one
Zengjing Granule: take Clinical isolation staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, albicans strain a little, be inoculated in respectively in broth bouillon, cultivates 18h for 37 DEG C。(adding 20% calf serum in B-mode micrococcus scarlatinae broth bouillon) takes the 18h each bacterial strain nutrient broth culture cultivated, and makes 10-3 with nutrient broth and dilutes for testing。
Doubling dilution (test tube method): take sterilizing test tubes 11,1st pipe adds nutrient broth fluid medium 1.6mL, all the other are pipe 1mL often, take the present invention respectively and add in the first pipe, 1mL to the 2nd pipe is taken after mixing, diluting successively, the 10th pipe sucking-off 1mL discards, and the 11st pipe is not added with hippophae rhamnoides soy as comparison。Often pipe adds dilution bacterium solution 0.1mL, cultivates 24h for 37 DEG C, takes out and observe bacterial growth situation。As hippophae rhamnoides soy pipe is muddy, namely represent bacterial growth, for examination hippophae rhamnoides soy without bacteriostasis;As limpid in hippophae rhamnoides soy pipe, represent that bacterial growth is suppressed, the hippophae rhamnoides soy of the greatest dilution of its energy bacteria growing inhibiting, it is the minimal inhibitory concentration (MIC) of this hippophae rhamnoides soy。By the limpid each pipe transferred species broth agar plates of hippophae rhamnoides soy meat soup, cultivate 24h and observe, be still the bacteriocidal concentration of this hippophae rhamnoides soy without the minimum hippophae rhamnoides soy concentration of bacterial growth。
Take penicillin sodium (being made into 160,000 units/mL) as stated above, to dilute successively, cultivate, make positive control。
Experimental result shows: clinical isolates staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, Candida albicans are respectively provided with stronger bacteriostasis by the present invention, and MIC is at 5mg~500mg/mL, it was shown that the present invention has vitro antibacterial activity。In Table 1。
Table 1 antibacterial activity in vitro of the present invention (n=2)
Experiment conclusion: clinical isolates staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, Candida albicans are respectively provided with stronger bacteriostasis by the present invention, MIC is at 5mg~200mg/mL, show that the present invention has vitro antibacterial activity, and effect is more effective than only using the soy sauce that Fructus Hippophae is configured to。

Claims (2)

1. a hippophae rhamnoides soy, it is characterised in that be made up of the raw material of following weight portion: Fructus Hippophae 18 parts, Folium Bambusae Herba Reineckeae Carneae 20 parts, Herba Crepidis hookerianae 18 parts, Fructus Phyllanthi 10 parts, Herba Houttuyniae 12 parts, sky, sea dark soy sauce 200 parts, Mel 50 parts, white sugar 50 parts。
2. hippophae rhamnoides soy as claimed in claim 1, it is characterized in that, preparation method comprises the following steps: take Fructus Hippophae, Folium Longan, Fructus Alpinia Japanicae, Caulis et Folium Hyptidis suaveolentis fruit, Herba Lophatheri, add the water of 10 times of weight of medical material, soak 0.5h, decoct 1 hour, filter, medicinal residues add the water of 8 times of weight of medical material, decoct 1 hour, medicinal liquid leaches, merge twice decoction liquor, being evaporated to 60 DEG C of relative densities is 1.05, adding ethanol makes the percent by volume of alcohol content reach 75%, stirring, stand, filter, when filtrate reduced in volume is to 65 DEG C, relative density is 1.25, and reclaim ethanol, obtain concentrated solution, again with sky, sea dark soy sauce, Mel and white sugar mixing, it is sufficiently stirred for and makes white sugar dissolve, mixing。
CN201610097340.XA 2016-02-22 2016-02-22 Hippophae rhamnoides soybean sauce and production method thereof Pending CN105685939A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610097340.XA CN105685939A (en) 2016-02-22 2016-02-22 Hippophae rhamnoides soybean sauce and production method thereof

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Application Number Priority Date Filing Date Title
CN201610097340.XA CN105685939A (en) 2016-02-22 2016-02-22 Hippophae rhamnoides soybean sauce and production method thereof

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CN105685939A true CN105685939A (en) 2016-06-22

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Application publication date: 20160622