CN110151938A - A kind of new application of ampelopsis grossedentata preparation - Google Patents
A kind of new application of ampelopsis grossedentata preparation Download PDFInfo
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A61K36/88—Liliopsida (monocotyledons)
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention belongs to traditional Chinese medicine health care product fields, and in particular to a kind of new application of ampelopsis grossedentata preparation.The compound preparation that ampelopsis grossedentata preparation of the present invention is made of vine tea, galangal, Poria cocos, four taste raw material of mung bean; the ampelopsis grossedentata preparation has certain protective effect to Alcoholic mucosal lesion rat model; the gastric juice amount of Alcoholic mucosal lesion rat can be significantly reduced; increase gastric juice pH; mitigate ulcerative lesions, has the function of preferably protecting Alcoholic mucosal lesion.Also, ampelopsis grossedentata preparation of the present invention has many advantages, such as that at low cost, preparation process is simple, easy to carry and convenient for taking.
Description
Technical field
The invention belongs to traditional Chinese medicine health care product fields, and in particular to a kind of new application of ampelopsis grossedentata preparation.
Background technique
Drinking beyond one's capacity can cause the intracorporal acetaldehyde of people that cannot be completely broken down, and make one generation nausea after drinking and be intended to spit, go into a coma
The drunk symptom such as discomfort.Show that somatization can have fatigue, headache, thirsty, dizziness, stomach trouble, Nausea and vomiting, insomnia, hand tremor
It is raised and lowered with blood pressure；Mental symptom includes acute anxiety, irritability, extrasensitivity, depression or sense of guilt.Long-term alcohol
It can cause chronic gastritis.The mucosal lesion disease incident of crowd of drinking is apparently higher than non-alcoholic crowd.Mucosal lesion with
Gastric acid secretion is excessively, helicobacter pylori (H.pylori) infects, non-steroidal anti-inflammatory drugs (NSAID) inhibits Cycloxygenase (COX)
Activity and the active oxygen (ROS) of generation cause the factors such as oxidative stress related.Alcohol is the key factor of mucosal lesion
One of, alcohol includes: that alcohol can interfere stomach lining secretory activity to the main mechanism of mucosal lesion, changes the permeability of cell
And stomach mucus is consumed, the pH value of normal gastric juice can reduce；Alcohol can induce the lipid peroxidation of stomach lining, generate oxygen radical,
Damage stomach lining.
Patent of invention protect liver tune stomach composition and its preparation method and application (CN201910124081.9) is disclosed by the heart of a lotus seed
The health care product that the seed of jog's tears, Gan Li, radish seed, campanulaceae, the tuber of dwarf lilyturf, Schisandra chinensis, semen armeniacae amarae and Radix Glycyrrhizae form, protect liver tune stomach combination
Each raw material scientific compatibility in object, synergistic, Small side effects, securely and reliably.But Halth-care composition disclosed in the invention
Complicated component, and the price of some drugs is higher, and the health care product higher cost prepared, price goes up therewith, Wu Fapu
And it is public.Doctoral thesis " Radix Astragali-pueraria lobata medicine is to the protection to alcohol induced gastric mucosa damage " (2015), has studied Huang
Stilbene-protective effect of the pueraria lobata medicine to alcohol induced gastric mucosa damage, wherein the compatibility of Radix Astragali pueraria lobata 3:1 is for protecting gastric mucosa
The effect of damage is better than other compatibility groups, can mitigate the pathology damages such as gastric bleeding caused by ethyl alcohol, oedema, erosion.
Inventor has conducted extensive research early period, applied for that patent of invention one kind is anti-intoxication on December 10th, 2014, relieved the effect of alcohol,
The preparation and its preparation process (CN201410751437.9) of liver protection, the invention using the health food of integration of drinking and medicinal herbs or food as
Raw material, is prepared for a kind of anti-intoxication, relieving alcoholism and protecting livers preparations, said preparation have the function of it is good it is anti-intoxication, relieve the effect of alcohol and liver protection.It should
The compound preparation that anti-intoxication, relieving alcoholism and protecting livers the preparations of invention are made of vine tea, galangal, Poria cocos, four taste raw material of mung bean.Its
In, for vine tea as natural products, toxic side effect is small, and a large amount of Flavonoid substances are contained in vine tea, is releasing ethylism, prevention
There is special efficacy in terms of alcoholic liver, fatty liver and the disease incidence for inhibiting liver cell to deteriorate, reduce liver cancer, protection liver；It is high
Galanga nature and flavor are hot, enter taste warp, the function with eliminating cold to stop pain, warming middle energizer to arrest vomiting, recording galangal in " new compilation of materia medica " can " warm stomach
Cold dispelling helps digestion and sobers up, controls stomach protein crymodynia ", there is certain antialcoholism action；Poria cocos have clearing damp and promoting diuresis, invigorating the spleen, calming heart function
Effect can promote alcohol in human body with the discharge of urine, conducive to sobering up.Mung bean has clearing heat and detoxicating effect, and wine can be effectively relieved
Dizziness, the symptoms such as or not enunciating afterwards.
Inventor is it was unexpectedly observed that the ampelopsis grossedentata preparation there is certain protection to make Alcoholic mucosal lesion rat model
With can significantly reduce the gastric juice amount of Alcoholic mucosal lesion rat, increase gastric juice pH, mitigate ulcerative lesions, have more
The effect of good protection Alcoholic mucosal lesion.Therefore, the present invention provides a kind of ampelopsis grossedentata preparations in preparation prevention or treatment
Application in Alcoholic mucosal lesion drug or health care product.
Summary of the invention
The purpose of the present invention is to provide a kind of new applications of ampelopsis grossedentata preparation.
The technical solution adopted by the invention is as follows:
A kind of application of ampelopsis grossedentata preparation in preparation prevention or treatment mucosal lesion drug or health care product, the vine tea
Preparation is made of the raw material of following parts by weight: 20-50 parts of vine tea, 5-15 parts of galangal, 10-40 parts of mung bean, 10-40 parts of Poria cocos.
Preferably, the ampelopsis grossedentata preparation is made of the raw material of following parts by weight: 30-50 parts of vine tea, 5-15 parts of galangal,
15-35 parts of mung bean, 10-40 parts of Poria cocos.
Preferably, the ampelopsis grossedentata preparation is made of the raw material of following parts by weight: 30 parts of vine tea, 5 parts of galangal, and mung bean 30
Part, 35 parts of Poria cocos.
Preferably, the ampelopsis grossedentata preparation is made of the raw material of following parts by weight: 35 parts of vine tea, 10 parts of galangal, and mung bean
30 parts, 25 parts of Poria cocos.
Preferably, the ampelopsis grossedentata preparation is made of the raw material of following parts by weight: 30 parts of vine tea, 15 parts of galangal, and mung bean
35 parts, 20 parts of Poria cocos.
Preferably, the ampelopsis grossedentata preparation is made of the raw material of following parts by weight: 40 parts of vine tea, 5 parts of galangal, and mung bean 25
Part, 30 parts of Poria cocos.
Preferably, the mucosal lesion is Alcoholic mucosal lesion.
A kind of application of ampelopsis grossedentata preparation in preparation prevention or treatment Alcoholic mucosal lesion drug or health care product, it is described
Ampelopsis grossedentata preparation preparation method the following steps are included:
A, each raw material is weighed by formula；
B, vine tea is added in the water of 5-15 times of its quality and impregnates 10-60min, refluxing extraction 1-3 times at 50-100 DEG C,
Each reflux extracting time is 30-120min, 200 mesh filter clothes of mistake after each refluxing extraction, cold under the conditions of 4 DEG C after merging filtrate
Hiding for 24 hours, obtains material A；
C, material A described in step b is centrifuged 5-15min under the conditions of 2000-4000r/min, obtain sediment and
Supernatant with 0.1-2 times of water washing precipitate of supernatant quality, and is centrifuged 5-15min under the conditions of 2000-4000r/min,
It repeats 3-5 times, finally obtains and be deposited in 60-100 DEG C of drying, obtain material B；
D, Poria cocos, galangal, mung bean are obtained into mixed material after mixing, 5-15 times of its quality is added in mixed material
Water in, then refluxing extraction 1-3 times at 50-100 DEG C, each refluxing extraction 30-120min, mistake after each refluxing extraction
200 mesh filter clothes, it is dry at 60-100 DEG C after merging filtrate concentration, obtain material C.
E, the material B that step c is obtained is uniformly mixed to obtain mixed material D with the material C that step d is obtained, with mixture
Expect D as active ingredient, using in pharmacy customary adjuvant and technique corresponding preparation is made.
Preferably, the ampelopsis grossedentata preparation is any one of chewable tablet, tablet, capsule and granule.
Preferably, the ampelopsis grossedentata preparation is granule.
The beneficial effects of the present invention are: the ampelopsis grossedentata preparation that the present invention is claimed, relative to (the beautiful pearl of positive controls 1
Obtain happy), positive controls 2 (Cimetidine), positive controls 3 (Radix Astragali-pueraria lobata Chinese medicine liquid) positive controls 4 (galangal),
It can significantly reduce the gastric juice amount of Alcoholic mucosal lesion rat, increase gastric juice pH, mitigate ulcerative lesions, have and preferably protect
Protect the effect of Alcoholic mucosal lesion；Meanwhile the ampelopsis grossedentata preparation has easy to carry and use, low in cost, production
The advantages that simple process.
Specific embodiment
The present invention is specifically illustrated below in conjunction with specific embodiment, but protection scope of the present invention is not
It is confined to following embodiment.
The ampelopsis grossedentata preparation 1 of a kind of prevention of embodiment 1 or treatment mucosal lesion
A kind of ampelopsis grossedentata preparation prevented or treat mucosal lesion, is made of the raw material of following parts by weight: 30 parts of vine tea, high
5 parts of galanga, 30 parts of mung bean, 35 parts of Poria cocos.
The preparation process of above-mentioned ampelopsis grossedentata preparation, comprising the following steps:
A, each raw material is weighed by formula；
B, 60min will be impregnated in the water that 5 times of its quality is added in vine tea, refluxing extraction 1 time at 50 DEG C, when refluxing extraction
Between be 30min, 200 mesh filter clothes are crossed after refluxing extraction, filtrate refrigerates for 24 hours under the conditions of 4 DEG C, obtains material A；
C, material A described in step b is centrifuged under the conditions of 2000r/min 15min, obtains sediment and supernatant,
With 0.1 times of water washing precipitate of supernatant quality, and it is centrifuged 15min under the conditions of 4000r/min, is repeated 3 times, finally obtains
80 DEG C of dryings are deposited in, material B is obtained；
D, Poria cocos, galangal, mung bean are obtained into mixed material after mixing, 15 times of its quality is added in mixed material
In water, then refluxing extraction 3 times at 100 DEG C, each refluxing extraction 30min crosses 200 mesh filter clothes after each refluxing extraction, closes
And it is dry at 100 DEG C after filtrate concentration, obtain material C；
E, the material B that step c is obtained is uniformly mixed to obtain mixed material D with the material C that step d is obtained；
D, be prepared into chewable tablet by the formula and technique of following weight percent: by 50% mixed material D, 40% is solvable
Property starch, 9% sucrose is pelletized with ethyl alcohol softwood, and dry, whole grain adds 1% magnesium stearate, is pressed into prevention or treatment stomach
The chewable tablets of mucosa injury.
The ampelopsis grossedentata preparation 2 of a kind of prevention of embodiment 2 or treatment mucosal lesion
A kind of ampelopsis grossedentata preparation prevented or treat mucosal lesion, is made of the raw material of following parts by weight: 35 parts of vine tea, high
10 parts of galanga, 30 parts of mung bean, 25 parts of Poria cocos.
The preparation process of above-mentioned ampelopsis grossedentata preparation, comprising the following steps:
A, each raw material is weighed by formula；
B, 10min will be impregnated in the water that 10 times of its quality is added in vine tea, refluxing extraction 3 times at 80 DEG C flow back every time
Extraction time is 120min, and 200 mesh filter clothes are crossed after each refluxing extraction, refrigerates for 24 hours, obtains under the conditions of 4 DEG C after merging filtrate
Material A；
C, material A described in step b is centrifuged under the conditions of 4000r/min 10min, obtains sediment and supernatant,
With 1 times of water washing precipitate of supernatant quality, and it is centrifuged 10min under the conditions of 2000r/min, is repeated 5 times, it is heavy to finally obtain
It forms sediment in 60 DEG C of dryings, obtains material B；
D, Poria cocos, galangal, mung bean are obtained into mixed material after mixing, 10 times of its quality is added in mixed material
In water, then refluxing extraction 1 time at 80 DEG C, refluxing extraction 60min crosses 200 mesh filter clothes after refluxing extraction, after filtrate concentration
It is dry at 80 DEG C, obtain material C.
E, the material B that step c is obtained is uniformly mixed to obtain mixed material D with the material C that step d is obtained；
D, be prepared into tablet by the formula and technique of following weight percent: by 70% mixed material D, 29% starch is used
Ethyl alcohol softwood is pelletized, dry, and whole grain adds 1% talcum powder, is pressed into prevention or is treated the tablet of mucosal lesion.
The ampelopsis grossedentata preparation 3 of a kind of prevention of embodiment 3 or treatment mucosal lesion
A kind of ampelopsis grossedentata preparation prevented or treat mucosal lesion, is made of the raw material of following parts by weight: 30 parts of vine tea, high
15 parts of galanga, 35 parts of mung bean, 20 parts of Poria cocos.
The preparation process of above-mentioned ampelopsis grossedentata preparation, comprising the following steps:
A, each raw material is weighed by formula；
B, 40min will be impregnated in the water that 15 times of its quality is added in vine tea, refluxing extraction 3 times at 100 DEG C flow back every time
Extraction time is 80min, and 200 mesh filter clothes are crossed after each refluxing extraction, refrigerates for 24 hours under the conditions of 4 DEG C after merging filtrate, obtains object
Expect A；
C, material A described in step b is centrifuged under the conditions of 3000r/min 5min, obtains sediment and supernatant, used
The water washing precipitate that 2 times of supernatant quality, and it is centrifuged 5min under the conditions of 4000r/min, it is repeated 4 times, finally obtains precipitating
In 100 DEG C of dryings, material B is obtained；
D, Poria cocos, galangal, mung bean are obtained into mixed material after mixing, 5 times of its quality is added in mixed material
In water, then refluxing extraction 3 times at 50 DEG C, each refluxing extraction 120min crosses 200 mesh filter clothes after each refluxing extraction, closes
And it is dry at 60 DEG C after filtrate concentration, obtain material C.
E, the material B that step c is obtained is uniformly mixed to obtain mixed material D with the material C that step d is obtained；
D, granule is prepared by the formula and technique of following weight percent: by 30% mixed material D, 55% paste
Softwood is made with ethyl alcohol in essence, 15% sucrose, pelletizes, and dry, whole grain is to get the granule for preventing or treating mucosal lesion.
The ampelopsis grossedentata preparation 4 of a kind of prevention of embodiment 4 or treatment mucosal lesion
A kind of ampelopsis grossedentata preparation prevented or treat mucosal lesion, is made of the raw material of following parts by weight: 40 parts of vine tea, high
5 parts of galanga, 25 parts of mung bean, 30 parts of Poria cocos.
The preparation process of above-mentioned ampelopsis grossedentata preparation, comprising the following steps:
A, each raw material is weighed by formula；
B, 40min will be impregnated in the water that 15 times of its quality is added in vine tea, refluxing extraction 2 times at 100 DEG C flow back every time
Extraction time is 80min, and 200 mesh filter clothes are crossed after each refluxing extraction, refrigerates for 24 hours under the conditions of 4 DEG C after merging filtrate, obtains object
Expect A；
C, material A described in step b is centrifuged under the conditions of 3000r/min 5min, obtains sediment and supernatant, used
The water washing precipitate that 2 times of supernatant quality, and it is centrifuged 5min under the conditions of 3000r/min, it is repeated 4 times, finally obtains precipitating
In 80 DEG C of dryings, material B is obtained；
D, Poria cocos, galangal, mung bean are obtained into mixed material after mixing, 5 times of its quality is added in mixed material
In water, then refluxing extraction 2 times at 80 DEG C, each refluxing extraction 120min crosses 200 mesh filter clothes after each refluxing extraction, closes
And it is dry at 100 DEG C after filtrate concentration, obtain material C.
E, the material B that step c is obtained is uniformly mixed to obtain mixed material D with the material C that step d is obtained；
D, mixed material D is prepared into softwood with ethyl alcohol, pelletized, drying, being fitted into hard capsule can must prevent or treat
The capsule of mucosal lesion.
5 ampelopsis grossedentata preparation of embodiment is used to prevent the research of mouse Alcoholic mucosal lesion
1. medicine ordinance
Ampelopsis grossedentata preparation 1: prescription proportion described in embodiment 1, the granule prepared according to 3 the method for embodiment；Vine tea
Preparation 2: prescription proportion as described in example 2, the granule prepared according to 3 the method for embodiment；Ampelopsis grossedentata preparation 3: by implementing
The proportion of prescription described in example 3, the granule prepared according to 3 the method for embodiment；Ampelopsis grossedentata preparation 4: by medicine as described in example 4
Side's proportion, the granule prepared according to 3 the method for embodiment；Bismuth potassium citrate: bismuth potassium citrate is configured to suspension；Radix Astragali-
Pueraria lobata Chinese medicine liquid: Radix Astragali and pueraria lobata are mixed by weight 3:1, decocted 3 times with 10 times of amount water, each 30min, collecting decoction obtains
Radix Astragali pueraria lobata Chinese medicine liquid；Galangal group: weighing galangal identical with crude drug amount in each ampelopsis grossedentata preparation, and being added to its quality is 5
Times water in, then refluxing extraction 3 times at 50 DEG C, each refluxing extraction 120min crosses the filter of 200 mesh after each refluxing extraction
Cloth, it is dry at 60 DEG C after merging filtrate concentration, galangal rhizome extract is obtained, the used time is faced, is configured with physiological saline.
2. grouping and administration
180-220g, male Wistar rat 100 (purchase is in Lanzhou biological products Co., Ltd animal experimental center),
10 groups are randomly divided into, control group: physiological saline 10ml/kg；Model group: after physiological saline 10ml/kg stomach-filling 14d, 56 ° of Red Stars two
Pot 1ml/ stomach-filling of head；Vine tea group: 1 group of ampelopsis grossedentata preparation (is formulated, dosage is calculated as 1g/kg with crude drug amount) described in embodiment 1, rattan
2 groups of tea formulation (is formulated, dosage is calculated as 1g/kg with crude drug amount) described in embodiment 2, and 3 groups of ampelopsis grossedentata preparation (are matched described in embodiment 3
Side, dosage are calculated as 1g/kg with crude drug amount), 4 groups of ampelopsis grossedentata preparation (is formulated, dosage is calculated as 1g/ with crude drug amount described in embodiment 4
Kg), after stomach-filling 14d, 56 ° of Red Star strong, colourless liquor distilled from sorghum stomach-fillings, the same model group of dosage；Positive controls 1: bismuth potassium citrate suspension, 1g/kg
After stomach-filling 14d, 56 ° of Red Star strong, colourless liquor distilled from sorghum stomach-fillings, the same model group of dosage；Positive controls 2: Cimetidine, 10ml/kg stomach-filling 14d
Afterwards, 56 ° of Red Star strong, colourless liquor distilled from sorghum stomach-fillings, the same model group of dosage；Positive controls 3: Radix Astragali-pueraria lobata Chinese medicine liquid, dosage is with crude drug amount
It is calculated as 1g/kg, after stomach-filling 14d, 56 ° of Red Star strong, colourless liquor distilled from sorghum stomach-fillings, the same model group of dosage；Galangal group: galangal extracting solution, administration
Amount is calculated as 1g/kg with crude drug amount, after stomach-filling 14d, 56 ° of Red Star strong, colourless liquor distilled from sorghum stomach-fillings, and the same model group of dosage.
3. processing
Before last dose, Rat Fast for 24 hours, prohibits water 8h, after the last administration 1h, each group rat 0.35ml/kg intraperitoneal injection
10% chloraldurate, is anaesthetized, abdominal aortic blood, centrifuging and taking serum, takes full stomach, is collected gastric juice and is measured Gastric pH, into
The observation of row gross specimen, -20 DEG C of juxtaposition spare.
4. index determining
4.1 body weight determination
The weight of rat is measured before administration daily.
The measurement of 4.2 rat stomach liquid measure stomach function regulating acid values
Gastric content is poured out in graduated centrifuge tube, collects gastric juice, and observe the degree and form of mucosal lesion.It will receive
The gastric juice collected is centrifuged 10min in centrifuge 1500r/min, supernatant is taken, using titration measuring total acidity.
The detection of 4.3 gastric tissue ulcer indexes
It after taking out body of stomach, cuts off along big curved side, bursts with ruler and magnifying glass measurement ulcer size and by Guth criterion calculation
Ulcer index: spot erosion remembers 1 point；Rotten to the corn length < lmm remembers 2 points；1mm~2mm remembers 3 points；2mm~3mm remembers 4 points；>=3mm note 5
Point, score value × 2 when width > 1mm.
4.4 macropathology semi-quantitative analyses
According to lesion area ratio shared by entire stomach lining, diagnosis sxemiquantitative is no abnormality seen (0 point), Minimal change
(1 point), slight lesion (2 points), moderate lesion (3 points) and severe lesion (4 points).
4.5 pathologic examinations and scoring
Gross examination finishes, and the position of every animal mucosal lesion most serious is cut, 10% formalin is fixed on,
Conventional film-making, HE dyeing, under the microscope.Select the positive cross section of stomach lining, the regional observation including mucous membrane holostrome.It is tired according to lesion
And the depth of mucous layer is to determine the main foundation of mucosa injury severity, it is proposed that lesion is divided into no abnormality seen (0 point), light
(2 points: it is glutinous that 1/3 < mucous membrane of mucous layer is damaged depth < for micro- lesion (1 point: mucous membrane is damaged depth < mucous layer 1/3), slight lesion
Film layer 2/3), (4 points: mucosa injury not only involves for moderate lesion (3 points: mucous membrane is damaged depth > mucous layer 2/3) and severe lesion
Holostrome, and the lesions such as visible bleeding of submucosa), observe lesion type.
4.6 serum NOs, the measurement of SOD, MDA, GSH level
Take above-mentioned steps 3 to be centrifuged resulting serum, using serum reagent box measurement rat blood serum in NO (nitric oxide),
The level of SOD (superoxide dismutase), MDA (malonaldehyde) and GSH (glutathione).
ENOS (eNOS), nNOS (Neuronal Nitric Oxide Synthase) and COX-2 in 4.7 gastric tissues
The mRNA of (cyclooxygenase-2 gene) expresses measurement
Above-mentioned spare gastric tissue is taken, is shredded, is homogenized with the gastric tissue that physiological saline is made 10%, centrifuging and taking supernatant,
The total serum IgE in gastric tissue is extracted with Trizol, total rna concentration is measured by micro-spectrophotometer, RNA stoste is diluted to 1 μ
g/mL.1 μ L (oligo) Primer dT and 10 μ L sterilizeds water for injection are added in 1 μ L of RNA solution after taking dilution, anti-in 65 DEG C
5min is answered, 4 μ L Reaction Buffer, 1 μ L Riblock Rnase Inhibitor and 2 μ L dNTP are added after taking-up
Mix is uniformly mixed, and adds 1 μ L Revert Aid M-mu/v RT, in 42 DEG C, 60min, 70 DEG C, 5min synthesis cDNA.So
10 μ L Master, 1 μ L upstream primer, 1 μ L downstream primer, 1 μ L cDNA template and 7 μ L sterile injections are added in eight unions afterwards
With water, centrifugation is mixed.It is finally putting into qPCR instrument and expands, amplification condition are as follows: 95 DEG C of denaturation 15min, 60 DEG C of annealing 1h, 95 DEG C
Extend 15min, then recycles 40 times.Using housekeeping gene GAPDH as reference gene, pass through 2-ΔΔCTCalculate the opposite of each gene
Expression quantity.
5. statistical analysis
All data are represented as average value ± standard deviation, carry out significance difference analysis using 17.0 software of SPSS,
Indicate that data have significant difference with P < 0.05.
6. result
The variation of 6.1 rat body weights
The weight indifference (P > 0.05) of each group rat before stomach-filling, after 14d is administered, the weight of every group of rat is relative to giving
(P < 0.05) is dramatically increased before medicine, but without significant difference (P > 0.05) between each group, is illustrated ampelopsis grossedentata preparation and other groups,
Toxic side effect will not be brought, does not influence the diet of rat, the normal growth of rat is will not influence, the results are shown in Table 1.
The changes of weight of the administration of table 1 front and back each group rat
Note:*P < 0.05, compared with rat body weight before stomach-filling
The measurement of 6.1 rat stomach liquid measure stomach function regulating acid values
Gastric juice amount hypersecretion or Gastric pH is too low can all aggravate mucosal lesion degree.As shown in Table 2, model group is big
The gastric juice amount of mouse is most, and the gastric juice amount of the rat of stomach-filling ampelopsis grossedentata preparation and positive control drug is substantially less than model group (P <
0.05), wherein the gastric juice amount of 3 groups of rats of ampelopsis grossedentata preparation closer to control group；The gastric juice pH of model group rats is minimum, stomach-filling rattan
The gastric juice pH of the rat of tea formulation and positive control drug is substantially less than model group (P < 0.05), wherein 3 groups of ampelopsis grossedentata preparation big
The gastric juice pH of mouse is closer to control group.Illustrate that ampelopsis grossedentata preparation can reduce the excessive secretion of rat stomach liquid measure, rat can be maintained
Normal pH in stomach, thus to a certain extent can less alcohol to the damaging action of stomach lining.
2 rat stomach liquid measure of table and gastric acid value
Note:*P < 0.05, compared with the control group；#P < 0.05, compared with model group
The detection of 6.2 gastric tissue ulcer indexes, gross examination of skeletal muscle scoring and histopathology scoring
The detection display of gastric tissue ulcer index: control rats mucosa surface is smooth, has no ulcer, ulcer index 0；
The visible many places of model group rats gastric tissue naked eyes are linear rotten to the corn, and rotten to the corn length majority is greater than 1mm in 3mm or more, width, and ulcer refers to
Number is (8.8 ± 1.40)；Ampelopsis grossedentata preparation group and positive controls make moderate progress compared with model group, wherein ampelopsis grossedentata preparation 2, rattan
The ulcer index of tea formulation 3 and positive controls rat is substantially less than model group (P < 0.05), and 3 groups of rats of ampelopsis grossedentata preparation
Ulcer index be lower than positive controls.
Gross examination of skeletal muscle scoring: control rats mucosa surface is smooth, no congested, rotten to the corn lesion, no abnormality seen；Model group
The visible many places stomach lining of gastric mucosa naked eyes is dotted, linear rotten to the corn and bleeding, and gross examination of skeletal muscle scoring is (3.5 ± 0.71)；Rattan
Tea formulation group and positive controls make moderate progress compared with model group, wherein ampelopsis grossedentata preparation 2, ampelopsis grossedentata preparation 3 and positive control
The gross examination of skeletal muscle scoring of group rat is substantially less than model group (P < 0.05), and the gross examination of skeletal muscle scoring of 3 groups of rats of ampelopsis grossedentata preparation
Lower than positive controls.
Histopathology scoring: lesion is divided into no abnormality seen (0 point), (1 point: mucous membrane is damaged depth < mucous layer to Minimal change
1/3), (3 points: mucous membrane is impaired deep for slight lesion (2 points: 1/3 < mucous membrane of mucous layer is damaged depth < mucous layer 2/3), moderate lesion
Spend > mucous layer 2/3) and severe lesion (4 points: mucosa injury not only involves holostrome, but also the diseases such as visible bleeding of submucosa
Become), observe lesion type.Control rats mucosa surface is smooth, no congested, rotten to the corn lesion, no abnormality seen；Model group rats
Stomach lining is damaged depth majority and is all larger than mucous layer 2/3, and histopathology scoring is (3.6 ± 0.52)；Ampelopsis grossedentata preparation group and the positive
Control group makes moderate progress compared with model group, wherein the tissue disease of ampelopsis grossedentata preparation 2, ampelopsis grossedentata preparation 3 and positive controls rat
Reason scoring is substantially less than model group (P < 0.05), and the histopathology scoring of 3 groups of rats of ampelopsis grossedentata preparation is lower than positive control
Group.
The detection of gastric tissue ulcer index, gross examination of skeletal muscle scoring and histopathology scoring show that ampelopsis grossedentata preparation can be to wine
Certain inhibiting effect is played in the damage of gastric mucosa caused by essence, and wherein appraisal result is shown in Table 3.
3 each group rat stomach tissue ulcer index of table, gross examination of skeletal muscle scoring and histopathology scoring
Note:#P < 0.05, compared with model group
6.3 serum NOs, the horizontal measurement result of SOD, MDA, GSH
As can be known from the results of Table 4, the NO content in model group rats serum, SOD vigor and GSH content be substantially less than pair
According to group (P < 0.05), MDA content is significantly higher than control group (P < 0.05)；In ampelopsis grossedentata preparation and positive controls rat blood serum
NO content, SOD vigor and GSH content are all remarkably higher than model group (P < 0.05), MDA content be substantially less than model group (P <
0.05), wherein NO content, SOD vigor, GSH content and the MDA content of 3 groups of rats of ampelopsis grossedentata preparation are compared in positive controls,
Closer to the level of control rats.Illustrate that ampelopsis grossedentata preparation can increase the NO content in rat blood serum, SOD vigor and GSH
Content reduces MDA content, and then protects gastric tissue not oxidative damage caused by by alcohol.
NO, SOD, MDA, GSH are horizontal in 4 each group rat blood serum of table
Note:*P < 0.05, compared with the control group；#P < 0.05, compared with model group
The mRNA of eNOS, nNOS and COX-2 express measurement in 6.4 gastric tissues
The generation of gastric ulcer is related with the extra-inhibitory of eNOS and nNOS, and Alcoholic mucosal lesion will lead to COX-
2 expression increases.Each group rat gastric tissue relevant inflammatory factors relative expression quantity is as shown in table 5 in this experiment, model group rats
The expression water of eNOS and nNOS is substantially less than control group (P < 0.05) in gastric tissue, and the expression of COX-2 is significantly higher than control
Group (P < 0.05)；The expression water of eNOS and nNOS is significantly higher than model group in ampelopsis grossedentata preparation and positive controls rat gastric tissue
(P < 0.05), the expression water of COX-2 are substantially less than model group (P < 0.05)；Wherein, compared with positive controls group, ampelopsis grossedentata preparation
Level of the expression of eNOS, nNOS and COX-2 closer to control rats in 3 groups of rat gastric tissues.As a result illustrate, rattan
Tea formulation can raise the expression of eNOS and nNOS in Alcoholic mucosal lesion rat gastric tissue, lower the expression of COX-2, right
Mucosal lesion caused by alcohol has certain protective effect.
ENOS, nNOS and COX-2 expression in 5 each group rat gastric tissue of table
Note:*P < 0.05, compared with the control group；#P < 0.05, compared with model group
7. conclusion
The present invention has inquired into ampelopsis grossedentata preparation disclosed by the invention to stomach lining using rat Alcoholic mucosal lesion as model
The protective effect of damage, as a result, it has been found that, vine tea system disclosed by the invention can significantly reduce the stomach of Alcoholic mucosal lesion rat
Liquid measure increases gastric juice pH, mitigates ulcerative lesions, has the function of preferably protecting Alcoholic mucosal lesion.Alcoholic stomach
Mucosa injury is one of the most common type mucosal lesion model, in ampelopsis grossedentata preparation disclosed by the invention to alcohol caused by stomach lining
On the basis of damage has prevention or therapeutic effect, also there is certain prevention to mucosal lesion caused by other factors or control
Treatment effect, and this be also skilled artisans appreciate that.
Claims (10)
1. a kind of application of ampelopsis grossedentata preparation in preparation prevention or treatment mucosal lesion drug or health care product, which is characterized in that
The ampelopsis grossedentata preparation is made of the raw material of following parts by weight: 20-50 parts of vine tea, 5-15 parts of galangal, and 10-40 parts of mung bean, Fu
10-40 parts of Siberian cocklebur.
2. a kind of ampelopsis grossedentata preparation as described in claim 1 is in preparation prevention or treatment mucosal lesion drug or health care product
Using, which is characterized in that the ampelopsis grossedentata preparation is made of the raw material of following parts by weight: 30-50 parts of vine tea, galangal 5-15
Part, 15-35 parts of mung bean, 15-40 parts of Poria cocos.
3. a kind of ampelopsis grossedentata preparation as claimed in claim 2 is in preparation prevention or treatment mucosal lesion drug or health care product
Using, which is characterized in that the ampelopsis grossedentata preparation is made of the raw material of following parts by weight: 30 parts of vine tea, 5 parts of galangal, and mung bean
30 parts, 35 parts of Poria cocos.
4. a kind of ampelopsis grossedentata preparation as claimed in claim 2 is in preparation prevention or treatment mucosal lesion drug or health care product
Using, which is characterized in that the ampelopsis grossedentata preparation is made of the raw material of following parts by weight: 35 parts of vine tea, 10 parts of galangal, and mung bean
30 parts, 25 parts of Poria cocos.
5. a kind of ampelopsis grossedentata preparation as claimed in claim 2 is in preparation prevention or treatment mucosal lesion drug or health care product
Using, which is characterized in that the ampelopsis grossedentata preparation is made of the raw material of following parts by weight: 30 parts of vine tea, 15 parts of galangal, and mung bean
35 parts, 20 parts of Poria cocos.
6. a kind of ampelopsis grossedentata preparation as claimed in claim 2 is in preparation prevention or treatment mucosal lesion drug or health care product
Using, which is characterized in that the ampelopsis grossedentata preparation is made of the raw material of following parts by weight: 40 parts of vine tea, 5 parts of galangal, and mung bean
25 parts, 30 parts of Poria cocos.
7. the ampelopsis grossedentata preparation as described in claim 1-6 is any is in preparation prevention or treatment mucosal lesion drug or health care product
Application, which is characterized in that the mucosal lesion be Alcoholic mucosal lesion.
8. a kind of ampelopsis grossedentata preparation as claimed in claim 7 is in preparation prevention or treatment mucosal lesion drug or health care product
Using, which is characterized in that the ampelopsis grossedentata preparation preparation method the following steps are included:
A, each raw material is weighed by formula；
B, vine tea is added in the water of 5-15 times of its quality and impregnates 10-60min, refluxing extraction 1-3 times at 50-100 DEG C, every time
Reflux extracting time is 30-120min, and 200 mesh filter clothes are crossed after each refluxing extraction, are refrigerated under the conditions of 4 DEG C after merging filtrate
For 24 hours, material A is obtained；
C, material A described in step b is centrifuged under the conditions of 2000-4000r/min 5-15min, obtains sediment and supernatant
Liquid with 0.1-2 times of water washing precipitate of supernatant quality, and is centrifuged 5-15min under the conditions of 2000-4000r/min, repeats
It 3-5 times, finally obtains and is deposited in 60-100 DEG C of drying, obtain material B；
D, Poria cocos, galangal, mung bean are obtained into mixed material after mixing, mixed material is added to the water of 5-15 times of its quality
In, then refluxing extraction 1-3 times at 50-100 DEG C, each refluxing extraction 30-120min crosses 200 mesh after each refluxing extraction
Filter cloth, it is dry at 60-100 DEG C after merging filtrate concentration, obtain material C.
E, the material B that step c is obtained is uniformly mixed to obtain mixed material D with the material C that step d is obtained, with mixed material D work
For active ingredient, using in pharmacy customary adjuvant and technique corresponding preparation is made.
9. a kind of ampelopsis grossedentata preparation as claimed in claim 8 is in preparation prevention or treatment Alcoholic mucosal lesion drug or health care
Application in product, which is characterized in that the ampelopsis grossedentata preparation is any one of chewable tablet, tablet, capsule and granule.
10. a kind of ampelopsis grossedentata preparation as claimed in claim 9 is in preparation prevention or treatment Alcoholic mucosal lesion drug or guarantor
Application in strong product, which is characterized in that the ampelopsis grossedentata preparation is granule.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101973976A (en) * | 2010-09-16 | 2011-02-16 | 湖北民族学院 | Method for extracting dihydromyricetin, ampelopsis grossedentata amylase and ampelopsis grossedentata polyphenol from ampelopsis grossedentata |
| CN104522639A (en) * | 2014-12-10 | 2015-04-22 | 甘肃陇神戎发药业股份有限公司 | Anti-intoxication, alcohol-expelling and liver-protection preparation and preparation process thereof |
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2019
- 2019-06-27 CN CN201910565421.1A patent/CN110151938B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101973976A (en) * | 2010-09-16 | 2011-02-16 | 湖北民族学院 | Method for extracting dihydromyricetin, ampelopsis grossedentata amylase and ampelopsis grossedentata polyphenol from ampelopsis grossedentata |
| CN104522639A (en) * | 2014-12-10 | 2015-04-22 | 甘肃陇神戎发药业股份有限公司 | Anti-intoxication, alcohol-expelling and liver-protection preparation and preparation process thereof |
Non-Patent Citations (1)
| Title |
|---|
| 陈立峰: "二氢杨梅素对大鼠免疫性慢性胃炎胃黏膜的保护作用及其机制", 《中国药理学与毒理学杂志》 * |
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