CN110150141A - A kind of method of purplish blue double-colored Waxy wheat quick breeding and identification - Google Patents
A kind of method of purplish blue double-colored Waxy wheat quick breeding and identification Download PDFInfo
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- CN110150141A CN110150141A CN201910592620.1A CN201910592620A CN110150141A CN 110150141 A CN110150141 A CN 110150141A CN 201910592620 A CN201910592620 A CN 201910592620A CN 110150141 A CN110150141 A CN 110150141A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The present invention relates to a kind of methods for improving Waxy wheat breeding efficiency of selection, it is characterized in that, using double cross or convergent cross, iodine staining and positive and negative baclccrossing techniques means quick breeding and the identification double-colored Waxy wheat strain of purplish blue, a kind of novel germ plasm resource of the double-colored Waxy wheat of purplish blue rich in 14 kinds of anthocyanin and amylopectin is formulated.The present invention is suitable for polymerizeing the glutinous trait gene (wx-A being located in the different chromosomes such as wheat 7AS, 4AL, 7DS, 3A, 7B and 4B1、wx‑B1And wx-D1), purple gene (Pk1And Pk2) and Blue Gene (Ba or Bk), initiative and the cultivation double-colored Waxy wheat of purplish blue, wheat health value and nutritional quality are improved, meets the needs of market is to health-nutrition green wheat products.
Description
Technical field
The present invention relates to a kind of methods of purplish blue double-colored Waxy wheat quick breeding and identification, belong to wheat genetic breeding technique
Field, it is specifically a kind of by double cross or convergent cross quick breeding and it is positive and negative backcrossing identification the double-colored Waxy wheat of purplish blue side
Method.
Background technique
Wheat seed has the colors such as red, white, purple and blue, and wheat with purple grain, blue-grain wheat are different from common wheat seed coat color
Also known as colorful wheat, because it rich in 14 kinds there is the important health cares such as removing interior free yl, raising immunity and inhibition cancer to make
With flavonoids anthocyanin, it has also become precious germ plasm resource.Blue-grain wheat is rich in 9 kinds of delphinidin anthocyanin etc. blue patterns
Glycosides, wheat with purple grain is rich in 7-9 kind purple anthocyanin such as Cyanidin anthocyanin, peonidin anthocyanin.Wheat with purple grain seed pericarp
In rich in purple anthocyanin and it is purple, pericarp is that maternal ovary wall is developed, female genotype determine fruit colour, performance
Maternal instinct delayed ingeritance effect out.Blue-grain wheat grain endosperm aleurone is blue rich in blue anthocyanin, and aleurone is by endosperm
Cells of superficial layer is developed, and aleurone is the outer layer endosperm of 3 times of bodies, shows Metaxenia.Since purple grain maternal instinct postpones
Hereditary and blue grain xenia and its dialogue grain are the reasons such as dominant, the purple grain pollen pollination F of white wheat1Still it is white grain, uses
Blue grain pollen pollination F1Show as blue grain.Research shows that Luo precious No. 1 is waited purple kernel Characters by dominant positioned at 2 pairs on 3A and 7B chromosome
Complementary genes Pk1、Pk2It controls, F after maternal instinct delayed ingeritance3Seed is 9 purple grains: 7 white grains, wherein Pk1May be located at 3A on it is red
Scytoblastema is because of R2It is allele, Pk2May with to be located at 7B purple stalk gene (pc) chain, thus between purple fruit skin and red skin and purple stalk
There is correlation；Common wheat indigo plant kernel Characters are by blue grain gene Ba or Bk Dominant gene and have apparent dosage effect, and Ba (comes
Derived from couchgrass) it is located on translocation chromosome 4BS4AgL, Bk (deriving from one grained wheat) is located on 4B substitution line chromosome.
Waxy wheat is that artificially creating amylopectin content is high novel with the wheat of amylose content pole (< 2%) in recent years
Germ plasm resource has the integrated qualities such as good food processing, pharmacy.Waxy wheat is straight on 7AS, 4AL and 7DS chromosome by being located at
Chain Starch synthesis Waxy protein gene lacks to form 3 pairs of waxy recessive gene (wx-A1, wx-B1 and wx-D1) controls, common small
Wheat hybridizes transformation F with Waxy wheat2It is 1/64 for Waxy Wheat Grains After Anthesis ratio, commonly uses 2%KI and 0.2%I2Iodine staining seed is crosscutting
Section 5-8min identifies that (Waxy wheat pinkiness or brownish red, partial waxy wheat and common wheat present blue, dark blue Waxy wheat
Color or black).
Wheat with purple grain, blue-grain wheat and Waxy wheat are all Special germplasm types, have not yet to see successfully breeding and contain purple
The relevant report of the double-colored Waxy wheat of purplish blue of grain gene and blue grain gene.There is colorful wheat stronger health value and Waxy wheat to have
Have a better nutritivity quality heterosis, and the gene for controlling waxy seed, purple and blue be located at 7AS, 4AL, 7DS, 3A, 7B and
In the different chromosomes such as 4B.Thus, waxy, purple and Blue Gene in different chromosomes are located at by technological means polymerization,
Formulate it is a kind of may be rich in 14 kinds of anthocyanin the double-colored Waxy wheat New idioplasm resource of purplish blue, can significantly improve its health value and
Nutritional quality is conducive to meet the needs of market is to health-nutrition green wheat products.
Summary of the invention
The object of the present invention is to provide a kind of methods of purplish blue double-colored Waxy wheat quick breeding and identification, mainly using double
The technological means quick breeding such as friendship or convergent cross, iodine staining and positive and negative backcrossing and the identification double-colored Waxy wheat strain of purplish blue, wound
Make a kind of novel germ plasm resource of the double-colored Waxy wheat of purplish blue rich in 14 kinds of anthocyanin and amylopectin.
The method that the present invention identifies the double-colored Waxy wheat of purplish blue using double cross or convergent cross quick breeding and positive and negative backcrossing, it is complete
At Waxy wheat Germplasm enhancement and new varieties (strain) breeding, the specific technical proposal is:
The first step is to choose wheat with purple grain and blue-grain wheat for maternal or male parent, with white grain Waxy wheat be maternal or male parent into
Row is orthogonal or reciprocal cross generates F1；
Second step is to use wheat with purple grain, blue-grain wheat orthogonal with white grain Waxy wheat or reciprocal cross F1Carry out double cross, iodine staining
Identify double cross F1The middle double-colored seed of purplish blue is waxy；
Or iodine staining identification wheat with purple grain, blue-grain wheat be orthogonal with white grain Waxy wheat or reciprocal cross F2It is purple glutinous small in seed
Wheat, blue Waxy wheat, then convergent cross F is generated with purple Waxy wheat and blue Waxy wheat convergent cross1；
Third step is from double cross F1Or convergent cross F1The double-colored Waxy wheat single plant of purplish blue is continuously selected in offspring, until F7Choosing
It selects seed coat color and stablizes the double-colored Waxy wheat strain of unseparated purplish blue；
4th step is with the double-colored Waxy wheat strain of purplish blue and the white positive and negative backcrossing of grain Waxy wheat, orthogonal F1Seed coat color and purplish blue
Double-colored Waxy wheat parent seed coat color is consistent, reciprocal cross F1Seed coat color is consistent with blue-grain wheat parent's seed coat color；
5th step is breeding and selection fine individual plant, cultivates the double-colored Waxy wheat new varieties of purplish blue or strain.
The present invention provides a kind of methods of purplish blue double-colored Waxy wheat quick breeding and identification, are suitable for polymerization and are located at wheat
Glutinous trait gene (wx-A in the different chromosomes such as 7AS, 4AL, 7DS, 3A, 7B and 4B1、wx-B1And wx-D1), purple gene (Pk1
And Pk2) and Blue Gene (Ba or Bk), initiative and the cultivation double-colored Waxy wheat of purplish blue, wheat health value and nutritional quality are improved,
Meets the needs of market is to health-nutrition green wheat products.
Specific embodiment
It is described in conjunction with the embodiments the specific technical solution of the present invention, the specific steps of following embodiment are as follows:
(1) parent chooses and hybridizes: choosing excellent wheat with purple grain, blue-grain wheat new varieties (strain) as maternal or father
This, is that maternal or male parent progress is orthogonal or reciprocal cross generates F with excellent white grain Waxy wheat new varieties (strain)1。
(2) double cross or the identification of convergent cross and iodine staining: with wheat with purple grain, the orthogonal or reciprocal cross F of white grain Waxy wheat1With
The orthogonal or reciprocal cross F of blue-grain wheat and white grain Waxy wheat1Carry out double cross, from a large amount of double cross F1Selection is than purple grain and indigo plant in seed
It is waxy to carry out iodine staining identification for the grain double-colored seed of the deeper purplish blue of parent's seed coat color.Or in wheat with purple grain, blue-grain wheat
Or reciprocal cross F orthogonal with white grain Waxy wheat respectively1Breed the F obtained2In seed, carries out iodine staining and identify purple Waxy wheat or indigo plant
Waxy wheat, then convergent cross F is generated with purple Waxy wheat and blue Waxy wheat convergent cross1。
(3) the double-colored single plant of breeding purplish blue: double cross F after breeding iodine staining identification1Or convergent cross F1Seed, from offspring
The double-colored Waxy wheat single plant of continuous selection purplish blue more deeper than purple grain and blue grain parent's seed coat color, until F7Choose seed coat color
Stablize the double-colored Waxy wheat strain of unseparated purplish blue (because of dominant single-gene F7Stablize 95% or more heredity individual).
(4) the positive and negative backcrossing identification of white grain Waxy wheat: to F7The double-colored Waxy wheat strain of purplish blue carries out positive and negative with white grain Waxy wheat
Backcrossing, utilizes wheat with purple grain maternal instinct delayed ingeritance and the double-colored Waxy wheat genotype of blue-grain wheat xenia CHARACTERISTICS IDENTIFICATION purplish blue.
As the double-colored Waxy wheat of purplish blue F orthogonal with white grain Waxy wheat1Seed coat color is consistent with the double-colored Waxy wheat parent seed coat color of purplish blue, anti-
Hand over F1When seed coat color is consistent with blue-grain wheat parent's seed coat color, show that purple is completed in the double-colored Waxy wheat strain of breeding purplish blue
The polymerization of grain, blue grain and waxy three genoid, successfully formulates the double-colored Waxy wheat germ plasm resource new material of purplish blue.
(5) the double-colored Waxy wheat of breeding purplish blue: the breeding double-colored Waxy wheat strain of purplish blue continues breeding and selects excellent purplish blue double-colored
Waxy wheat single plant cultivates the double-colored Waxy wheat new varieties (strain) of purplish blue.
It is specific:
Embodiment 1
The method of first set double cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 2
The method of second set of double cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 3
The method that third covers double cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 4
The method of 4th set of double cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 5
The method of 5th set of double cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 6
The method of 6th set of double cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 7
The method of 7th set of double cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 8
The method of 8th set of double cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 9
The method of 9th set of convergent cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 10
The method of tenth set of convergent cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 11
The method of tenth a set of convergent cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 12
The method of 12nd set of convergent cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 13
The method of 13rd set of convergent cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 14
The method of 14th set of convergent cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 15
The method of 15th set of convergent cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
Embodiment 16
The method of 16th set of convergent cross breeding and the positive and negative backcrossing identification double-colored Waxy wheat of purplish blue:
The present invention has introduced characteristic germ plasm resource Luo precious No. 1 and has waited blue-grain wheats and its derivatives such as wheat with purple grain, blue mark wheat
System, and the above method is applied, double cross or convergent cross are carried out with Waxy wheats Derivative lines such as Chongqing L-1,12LF6-12, has cultivated Chongqing
LF0The double-colored Waxy wheat strain of the purplish blue such as -1 more than 100, and the purple Waxy wheat of a batch and blue Waxy wheat new lines.
Claims (2)
1. a kind of method of purplish blue double-colored Waxy wheat quick breeding and identification, which is characterized in that utilize double cross or convergent cross, iodine
Liquid dyeing and positive and negative baclccrossing techniques means quick breeding and the identification double-colored Waxy wheat strain of purplish blue.
2. a kind of method of purplish blue double-colored Waxy wheat quick breeding and identification according to claim 1, which is characterized in that tool
Body includes following technical step:
The first step chooses wheat with purple grain and blue-grain wheat as maternal or male parent, is that maternal or male parent carries out just with white grain Waxy wheat
It hands over or reciprocal cross generates F1；
Second step, with wheat with purple grain, blue-grain wheat with white grain Waxy wheat orthogonal or reciprocal cross F1Double cross is carried out, iodine staining identification is double
Hand over F1The middle double-colored seed of purplish blue is waxy；Or iodine staining identification wheat with purple grain, blue-grain wheat and white grain Waxy wheat are orthogonal or anti-
Hand over F2Purple Waxy wheat, blue Waxy wheat in seed, then convergent cross F is generated with purple Waxy wheat and blue Waxy wheat convergent cross1；
Third step, from double cross F1Or convergent cross F1The double-colored Waxy wheat single plant of purplish blue is continuously selected in offspring, until F7Choose seed
The grain double-colored Waxy wheat strain of the unseparated purplish blue of colour stable；
4th step, with the double-colored Waxy wheat strain of purplish blue and the white positive and negative backcrossing of grain Waxy wheat, orthogonal F1Seed coat color is double-colored glutinous with purplish blue
Wheat parent seed coat color is consistent, reciprocal cross F1Seed coat color is consistent with blue-grain wheat parent's seed coat color；
5th step, breeding and selection fine individual plant, cultivate the double-colored Waxy wheat new varieties of purplish blue or strain.
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| CN201910592620.1A CN110150141A (en) | 2019-07-03 | 2019-07-03 | A kind of method of purplish blue double-colored Waxy wheat quick breeding and identification |
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| CN201910592620.1A CN110150141A (en) | 2019-07-03 | 2019-07-03 | A kind of method of purplish blue double-colored Waxy wheat quick breeding and identification |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111183894A (en) * | 2020-02-15 | 2020-05-22 | 四川农业大学 | Creating method of HMW-GS full-deletion waxy wheat |
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| CN101755668A (en) * | 2008-12-24 | 2010-06-30 | 周扬家 | Breeding method of purplish black waxy wheat |
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| CN106069729A (en) * | 2016-07-07 | 2016-11-09 | 福建农林大学 | A kind of selection of colorful wheat kind |
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Patent Citations (4)
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| CN101755668A (en) * | 2008-12-24 | 2010-06-30 | 周扬家 | Breeding method of purplish black waxy wheat |
| CN102283095A (en) * | 2011-06-22 | 2011-12-21 | 云南省农业科学院粮食作物研究所 | Method for breeding hybrid seeds of color waxy corn |
| CN106069729A (en) * | 2016-07-07 | 2016-11-09 | 福建农林大学 | A kind of selection of colorful wheat kind |
| CN106212268A (en) * | 2016-08-12 | 2016-12-14 | 漯河市农业科学院 | A kind of selection of disease-resistant wheat with purple grain strain of getting bumper crops |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111183894A (en) * | 2020-02-15 | 2020-05-22 | 四川农业大学 | Creating method of HMW-GS full-deletion waxy wheat |
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Application publication date: 20190823 |