CN110144423A - Depilating method associated with a kind of alkali enzyme - Google Patents
Depilating method associated with a kind of alkali enzyme Download PDFInfo
- Publication number
- CN110144423A CN110144423A CN201910541008.1A CN201910541008A CN110144423A CN 110144423 A CN110144423 A CN 110144423A CN 201910541008 A CN201910541008 A CN 201910541008A CN 110144423 A CN110144423 A CN 110144423A
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- CN
- China
- Prior art keywords
- enzyme
- animal skin
- alkali
- keratinase
- dipped
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C14—SKINS; HIDES; PELTS; LEATHER
- C14C—CHEMICAL TREATMENT OF HIDES, SKINS OR LEATHER, e.g. TANNING, IMPREGNATING, FINISHING; APPARATUS THEREFOR; COMPOSITIONS FOR TANNING
- C14C1/00—Chemical treatment prior to tanning
- C14C1/06—Facilitating unhairing, e.g. by painting, by liming
-
- C—CHEMISTRY; METALLURGY
- C14—SKINS; HIDES; PELTS; LEATHER
- C14C—CHEMICAL TREATMENT OF HIDES, SKINS OR LEATHER, e.g. TANNING, IMPREGNATING, FINISHING; APPARATUS THEREFOR; COMPOSITIONS FOR TANNING
- C14C1/00—Chemical treatment prior to tanning
- C14C1/06—Facilitating unhairing, e.g. by painting, by liming
- C14C1/065—Enzymatic unhairing
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention discloses depilating methods associated with a kind of alkali enzyme.It is reacted this method comprises: 1) Animal Skin to be epilated is dipped into alkali, keeping system pH in reaction process is alkalinity, then wipes off and become mildewed, and is cleaned；2) by step 1), treated that Animal Skin to be epilated is dipped into keratin enzyme solution, the pH value for adjusting keratinase enzymolysis liquid carries out enzyme digestion reaction I after being 7-8, it is dipped into neutral protein enzyme solution again, the pH value for adjusting neutral protease enzymolysis liquid carries out enzyme digestion reaction II after being 6-8, it cleans, shaving obtains finished product.It is lost hair or feathers using depilating method provided by the invention to animal fur, up to 98.2%, hair root part can be preferably removed plucking rate, can reduce cost, time saving and energy saving.
Description
Technical field
The present invention relates to a kind of depilating methods, more particularly, to depilating method associated with a kind of alkali enzyme.
Background technique
Currently used depilating method has grey alkaline process, enzyme process, hydrogen peroxide oxidation epilation etc., but there are problems that three:
First is that it is seriously polluted caused by chemical residue, second is that bio-chemical used in processing is not suitable for food processing,
Third is that depilation efficiency is lower.In ox-hide product processing, in order to keep cortex noresidue hair root etc., it is de- to generally use enzyme process
Hair.
Summary of the invention
The object of the present invention is to provide depilating methods associated with a kind of alkali enzyme.This method can guarantee to slough ox-hide surface residual
Hair root is stayed, preferable hair removal effect is reached.
A kind of claimed alkali and the enzyme-linked application in the depilation of Animal Skin.
A kind of composition for Animal Skin depilation, alkali and enzyme including independent packaging is also claimed in the present invention.
In above-mentioned application and composition, any one in aqueous solution of the alkali selected from sodium hydroxide and sodium bicarbonate；
The enzyme in neutral proteinase and keratinase any one.
Above-mentioned composition can also be only made of the alkali and enzyme of above-mentioned independent packaging.
The depilating method of Animal Skin provided by the invention, comprising:
1) Animal Skin to be epilated is dipped into alkali and is reacted, keeping system pH in reaction process is alkalinity, then is scraped
Fall to become mildewed, clean；
2) by step 1), treated that Animal Skin to be epilated is dipped into keratin enzyme solution, adjusts keratinase enzymolysis liquid
PH value carries out enzyme digestion reaction I after being 7-8, then is dipped into neutral protein enzyme solution, and the pH value for adjusting neutral protease enzymolysis liquid is
Enzyme digestion reaction II is carried out after 6-8, is cleaned, shaving obtains finished product.
In the step 1) of the above method, the alkali is selected from the aqueous solution of sodium hydroxide and sodium bicarbonate；
The mass percentage concentration of the alkali is 2-20%；Specially 3%；
In the reaction step, temperature is 18-60 DEG C；Specially 25 DEG C；Time is 2-120min；
In the adjusting pH value step, regulator is alkaline buffer；PH value after adjusting is 12-14；Specially 13.
In step 2), the dosage for impregnating keratin enzyme solution or neutral protein enzyme solution used every time is Animal Skin to be epilated
Tare weight amount.
In step 2), the enzyme activity of the keratinase is 1500-3000u/g；Specially 2000u/g；The keratin
The dosage of enzyme is that every 2-8kg Animal Skin to be epilated uses keratinase 300g-600g；Specially 500g；
The enzyme activity of the neutral proteinase is 1500-3000u/g；Specially 2000u/g；The use of the neutral proteinase
Amount is that every 2-8kg Animal Skin to be epilated uses neutral proteinase 300g-600g；Specially 500g.
In above-mentioned keratinase and neutral proteinase, the enzyme activity of protease is every g protease in 25 DEG C of thermotonuses 1
Enzyme amount required for 1 micromole substrate of conversion is a unit of activity (U) in minute
In the enzyme digestion reaction I step, temperature is 22-40 DEG C；Specially 38 DEG C；Time is 3-9h；
In the enzyme digestion reaction II step, temperature is 20-50 DEG C；Specially 38 DEG C；Time is 1.5-9h；Specially 3h.
The Animal Skin can be various Animal Skins, concretely ox-hide.
The all material that the above method is related to is cleaning, free of contamination material.
The above method may also include that before the step 1), and ox-hide is cut into small pieces, to be more conducive to lose hair or feathers.
It is lost hair or feathers using depilating method provided by the invention to animal fur, plucking rate is up to 98.2%, hair root part
It can preferably be removed, cost can be reduced, it is time saving and energy saving.
Detailed description of the invention
Fig. 1 is the Angus ox-hide not lost hair or feathers；
Fig. 2 is in reference examples with the effect picture after soaking with sodium hydroxide ox-hide shaving；
Fig. 3 is the top view after being lost hair or feathers with protease；
Fig. 4 is the side view after being lost hair or feathers with protease.
Specific embodiment
The present invention is further elaborated combined with specific embodiments below, but the present invention is not limited to following embodiments.Institute
State method is conventional method unless otherwise instructed.The raw material can obtain unless otherwise instructed from public commercial source.
Keratinase used in following embodiments is the product of one promise Biotechnology Co., Ltd of Zhejiang, and the date of manufacture is December 23 in 2018
Day, neutral proteinase used is the product of Cangzhou Xia Sheng biotechnology of enzymes Co., Ltd, lot number 20181118.
Embodiment 1
A kind of edible depilating method of beef cattle ox-hide, includes the following steps:
(1) the Angus ox-hide not lost hair or feathers shown in Fig. 1 is taken, stored frozen is spare after packaging；
(2) after cleaning up ox-hide defrosting, ox-hide is cut into small pieces；
(3) ox-hide is placed in slot, being dipped into the food grade sodium hydroxide solution of 3wt% (ensures ox-hide grain part
Sodium hydroxide can be all dipped into), 120min is reacted under conditions of temperature is 25 DEG C, alkaline buffer is added, and adjusts pH value
It is 13；
(4) it is wiped off after surface becomes mildewed with physical method, is left hair root part；
(5) ox-hide is cleaned, residual lye is washed off；
(6) the angle property aqueous solution of protease for being again 2000u/g by step 5) treated ox-hide 5kg is dipped into enzyme activity
In, adjusting enzymolysis liquid pH value is to 7, enzyme digestion reaction 3h under the conditions of 38 DEG C；It is 2000u/g's that ox-hide, which is dipped into enzyme activity, therewith
In neutral protein enzyme aqueous solution, adjusting enzymolysis liquid pH value is to 7, enzyme digestion reaction 3h under the conditions of 38 DEG C；Angle used egg is impregnated every time
The dosage of white enzyme solution or neutral protein enzyme solution is ox-hide weight to be epilated, to guarantee that ox-hide can be completely submerged in enzyme solution
Face；The dosage of the keratinase and neutral proteinase is 500g；
(7) take out ox-hide, after cleaning using scrape hair plates carry out physics depilation；Obtain finished product.
Fig. 3 and Fig. 4 is the side view and top view after being lost hair or feathers with protease, as seen from the figure, it can be seen that hair root portion
Divide and is divested.
Reference examples
For being lost hair or feathers with alkali and enzyme, exclusive use alkali concentration is 3wt%, temperature is reacted under conditions of being 25 DEG C
Effect such as Fig. 2 when 120min, hair root part can not be removed, and keratinase is used alone, enzyme activity 2000u/g, pH
Enzyme digestion reaction 3h under the conditions of 7,38 DEG C of value, when being lost hair or feathers exist become mildewed due to cause protease penetrate into pore when
Between relatively long, part pore is not affected by effect, and hair removal effect is poor.
For the step of using alkali or enzyme, if do not lost hair or feathers under the conditions of of the present invention, the depilation time is longer,
Hair removal effect is poor.
Claims (9)
1. alkali and the enzyme-linked application in the depilation of Animal Skin.
2. a kind of composition for Animal Skin depilation, alkali and enzyme including independent packaging.
3. application according to claim 1 or composition as claimed in claim 2, it is characterised in that: the alkali is selected from hydrogen
Any one in the aqueous solution of sodium oxide molybdena and sodium bicarbonate；
The enzyme in neutral proteinase and keratinase any one.
4. a kind of depilating method of Animal Skin, comprising:
1) Animal Skin to be epilated is dipped into alkali and is reacted, keeping system pH in reaction process is alkalinity, then wipes length off
Hair is cleaned；
2) by step 1), treated that Animal Skin to be epilated is dipped into keratin enzyme solution, adjusts the pH value of keratinase enzymolysis liquid
To carry out enzyme digestion reaction I after 7-8, then it is dipped into neutral protein enzyme solution, after the pH value for adjusting neutral protease enzymolysis liquid is 6-8
Enzyme digestion reaction II is carried out, is cleaned, shaving obtains finished product.
5. according to the method described in claim 4, it is characterized by: the alkali is selected from sodium hydroxide and carbon in the step 1)
The aqueous solution of sour hydrogen sodium；
The mass percentage concentration of the alkali is 2-20%；
In the reaction step, temperature is 18-60 DEG C；Time is 2-120min；
In the adjusting pH value step, regulator is alkaline buffer；PH value after adjusting is 12-14.
6. according to any method of claim 4 or 5, it is characterised in that: in the step 2), impregnate angle used egg every time
The dosage of white enzyme solution or neutral protein enzyme solution is the tare weight amount of Animal Skin to be epilated.
7. according to the method any in claim 4-6, it is characterised in that: in the step 2), the keratinase
Enzyme activity is 1500-3000u/g；The dosage of the keratinase is that every 2kg-8kg Animal Skin to be epilated uses keratinase
300g-600g；
The enzyme activity of the neutral proteinase is 1500-3000u/g；The dosage of the neutral proteinase is that every 2kg-8kg waits taking off
The Animal Skin of hair uses neutral proteinase 300g-600g.
8. according to the method any in claim 4-7, it is characterised in that: in the enzyme digestion reaction I step, temperature is
22-40℃；Time is 3-9h；
In the enzyme digestion reaction II step, temperature is 20-50 DEG C；Time is 1.5-9h.
9. any in application according to claim 1 or 3 or composition described in claim 2 or 3 or claim 4-8
The method, it is characterised in that: the Animal Skin is ox-hide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910541008.1A CN110144423A (en) | 2019-06-21 | 2019-06-21 | Depilating method associated with a kind of alkali enzyme |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910541008.1A CN110144423A (en) | 2019-06-21 | 2019-06-21 | Depilating method associated with a kind of alkali enzyme |
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| Publication Number | Publication Date |
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| CN110144423A true CN110144423A (en) | 2019-08-20 |
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ID=67596275
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910541008.1A Pending CN110144423A (en) | 2019-06-21 | 2019-06-21 | Depilating method associated with a kind of alkali enzyme |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111264597A (en) * | 2019-12-17 | 2020-06-12 | 中国农业科学院北京畜牧兽医研究所 | Slaughtering method of beef with skin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106048104A (en) * | 2016-07-28 | 2016-10-26 | 四川大学 | Alkaline complex unhairing enzyme preparation for tanning and application process of alkaline complex unhairing enzyme preparation |
| CN107893133A (en) * | 2017-11-09 | 2018-04-10 | 四川大学 | A kind of process hides ox-hide enzymatic depilation method |
-
2019
- 2019-06-21 CN CN201910541008.1A patent/CN110144423A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106048104A (en) * | 2016-07-28 | 2016-10-26 | 四川大学 | Alkaline complex unhairing enzyme preparation for tanning and application process of alkaline complex unhairing enzyme preparation |
| CN107893133A (en) * | 2017-11-09 | 2018-04-10 | 四川大学 | A kind of process hides ox-hide enzymatic depilation method |
Non-Patent Citations (1)
| Title |
|---|
| 王艺伦: "碱-酶脱牛皮、牛蹄毛工艺条件优化", 《甘肃农业大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111264597A (en) * | 2019-12-17 | 2020-06-12 | 中国农业科学院北京畜牧兽医研究所 | Slaughtering method of beef with skin |
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Application publication date: 20190820 |
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