CN110143830B - Application of peroxidase in compost, compost preparation method and application of compost - Google Patents

Application of peroxidase in compost, compost preparation method and application of compost Download PDF

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Publication number
CN110143830B
CN110143830B CN201910572829.1A CN201910572829A CN110143830B CN 110143830 B CN110143830 B CN 110143830B CN 201910572829 A CN201910572829 A CN 201910572829A CN 110143830 B CN110143830 B CN 110143830B
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compost
peroxidase
buffer solution
antibiotics
livestock
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CN110143830A (en
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董博
姜小凤
曾骏
刘晓伟
陈光荣
宋淑珍
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Shengfeng Yantai Agricultural Technology Co ltd
DRYLAND AGRICULTURE INSTITUTE GANSU ACADEMY OF AGRICULTURAL SCIENCES
Gansu Agricultural University
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Shengfeng Yantai Agricultural Technology Co ltd
DRYLAND AGRICULTURE INSTITUTE GANSU ACADEMY OF AGRICULTURAL SCIENCES
Gansu Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/80—Separation, elimination or disposal of harmful substances during the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00—Fertilisers from human or animal excrements, e.g. manure
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Toxicology (AREA)
  • Fertilizers (AREA)

Abstract

The invention relates to an application of peroxidase in compost, a compost preparation method and an application of compost. The method can improve the removal rate of antibiotics in the compost.

Description

Application of peroxidase in compost, compost preparation method and application of compost
Technical Field
The invention relates to an application of peroxidase in compost, a compost preparation method and an application of compost.
Background
The antibiotic is also called antibiotic, is a secondary metabolite produced by the growth of microbes such as actinomycetes, bacteria, fungi and the like to a certain stage or a similar or same substance prepared by directly adopting a chemical synthesis or semi-synthesis method, and has the effect of killing or inhibiting pathogenic bacteria within a certain concentration range. The usage amount of antibiotics in livestock and poultry breeding industry accounts for about 70 percent of the total production amount all over the world, and the usage amount of the antibacterial drugs in recent years in China reaches 6000 t. The antibiotics are rarely absorbed in intestinal tracts, only a small amount of antibiotics can be absorbed by organisms, non-toxic and harmless substances are generated in vivo through hydroxylation, cracking, glucoside acidification and other processes, about 60-90% of the antibiotics can be discharged out of the bodies along with excrement and urine in an original shape, and therefore a large amount of excrement containing the antibiotics is generated.
Most of the excrement is used in the farmland and even discharged into the water body along with the sewage. After being applied to soil, the antibiotics remained in the livestock and poultry manure not only destroy the ecological balance of microorganisms in the soil, but also enter a food chain through enrichment in plant bodies; antibiotics in the livestock and poultry excrement can enter a water body along with ways of seepage, loss and the like, so that the water environment is polluted and toxic effects are generated on organisms in the water environment, the residual antibiotics in the environment can induce bacteria to generate drug resistance or strong drug resistance bacteria, even induce the bacteria to generate drug resistance genes and transfer the drug resistance genes in the similar bacteria, and further harm is generated on human health.
The direct application of the livestock and poultry manure into the soil may cause diseases and pests and poison to seeds and seedlings, generally, the livestock and poultry manure needs to be thoroughly decomposed before being applied into a farmland, however, antibiotics have strong bacteriostatic and bactericidal properties, and are difficult to completely remove only by adopting a compost decomposition mode, and the removal rate is 65-85% for removing the antibiotics by adopting the compost mode at present, which is not satisfactory.
At present, no method for further improving the removal rate of antibiotics is available.
Disclosure of Invention
The invention provides an application of peroxidase in compost, a compost preparation method and an application of compost, and aims to further improve the removal rate of antibiotics.
In order to solve the technical problems, the invention adopts the following technical scheme:
use of peroxidase in composting.
Use of peroxidase in composting.
The preparation method of the compost comprises the steps of adding peroxidase into livestock and poultry manure containing antibiotics, and stacking for a period of time under a certain condition to obtain the compost without the antibiotics.
Further comprises adding catalase.
Further comprises adding a pH buffer solution; the pH of the pH buffer solution is 6.0-10.
Dissolving peroxidase in a pH buffer solution, and adding the solution into livestock and poultry manure containing antibiotics; or
Dissolving peroxidase and catalase in pH buffer solution, and adding into livestock and poultry feces containing antibiotic.
The certain conditions are that the temperature is 5-35 ℃ and the water content is 55-75%; and the stacking time is 15-180 days.
And also comprises turning the materials in the stacking process.
The mass ratio of the peroxidase to the pH buffer solution to the livestock and poultry manure is 0.5-15: 0-700: 1000.
The application of compost, the compost is applied to fertilizers or farmlands.
The invention has the following beneficial technical effects:
1. the invention can further remove antibiotics on the basis of compost.
2. The invention adds peroxidase, and removes the antibiotic by removing the phenol ring and the amino group in the antibiotic by the peroxidase.
3. The combination of peroxidase and catalase can remove antibiotics better.
4. According to the method, an acid-base buffer solution is added to prevent enzyme inactivation caused by excessive organic acid locally generated in the composting process; specifically, the polysaccharide compound is hydrolyzed into monosaccharide during composting, and the monosaccharide is rapidly decomposed into alcohol, acetic acid, oxalic acid and the like, so that excessive local acidity can poison and even inactivate enzyme.
5. The application adds peroxidase and catalase into pH buffer solution, can make buffer solution better protect the enzyme, also facilitates the use simultaneously.
Detailed Description
The present invention is further illustrated by the following specific examples.
Example 1
Use of peroxidase in composting.
The preparation method of the compost comprises the steps of adding peroxidase into livestock and poultry manure containing antibiotics, and stacking for a period of time under a certain condition to obtain the compost without the antibiotics.
The certain conditions are that the temperature is 22-24 ℃ and the water content is 60-62%; the stacking period is 60 days.
The mass ratio of the peroxidase to the water to the livestock and poultry manure is 3:400: 1000.
Example 2
Use of peroxidase and catalase in composting.
The preparation method of the compost comprises the steps of adding peroxidase and catalase into livestock and poultry manure containing antibiotics, and stacking for a period of time under certain conditions to obtain the compost without the antibiotics.
The certain conditions are that the temperature is 22-24 ℃ and the water content is 60-62%; the stacking period is 60 days.
The mass ratio of the peroxidase to the catalase to the water to the livestock and poultry manure is 3:1:399: 1000.
Example 3
Use of peroxidase and catalase in composting.
The preparation method of the compost comprises the steps of respectively adding peroxidase, catalase and pH buffer solution into livestock and poultry manure containing antibiotics, and stacking for a period of time under certain conditions to obtain the compost without the antibiotics.
The pH buffer solution is a phosphate buffer solution with the pH of 8.0.
The certain conditions are that the temperature is 22-24 ℃ and the water content is 60-62%; the stacking period is 60 days.
The mass ratio of the peroxidase to the catalase to the pH buffer solution to the livestock and poultry manure is 3:1:399: 1000.
Example 4
Use of peroxidase and catalase in composting.
The preparation method of the compost comprises the steps of dissolving peroxidase and catalase in a pH buffer solution, adding the mixed solution into livestock and poultry manure containing antibiotics, and stacking for a period of time under certain conditions to obtain the compost without the antibiotics.
The pH buffer solution is a phosphate buffer solution with the pH of 8.0.
The certain conditions are that the temperature is 22-24 ℃ and the water content is 60-62%; the stacking period is 60 days.
The mass ratio of the peroxidase to the catalase to the pH buffer solution to the livestock and poultry manure is 3:1:399: 1000.
Example 5
Use of peroxidase in composting.
The preparation method of the compost comprises the steps of dissolving peroxidase and catalase in a pH buffer solution, adding the solution into livestock and poultry manure containing antibiotics, and stacking the manure for a period of time under certain conditions to obtain the compost without the antibiotics.
Further comprises adding a pH buffer solution; the pH buffer solution is Tris-hydrochloric acid buffer solution, and the pH value is 8.5.
The certain conditions are that the temperature is 26-30 ℃ and the water content is 55-60%; the stacking period is 90 days.
And in the stacking process, turning over the fertilizer once every 5 days.
The compost is applied to fertilizers.
The mass ratio of the peroxidase to the catalase to the pH buffer solution to the livestock and poultry manure is 10:2:600: 1000.
Example 6
Use of peroxidase in composting.
The preparation method of the compost comprises the steps of adding peroxidase and catalase into livestock and poultry manure containing antibiotics, and stacking for a period of time under certain conditions to obtain the compost without the antibiotics.
Further comprises adding a pH buffer solution; the pH buffer solution is glycine-sodium hydroxide buffer solution, and the pH is 9.0.
Specifically, peroxidase and catalase are dissolved in a pH buffer solution and added to livestock and poultry manure containing antibiotics.
The certain conditions are that the temperature is 15-20 ℃ and the water content is 60-65%; the stacking period is 120 days.
And in the stacking process, turning over the fertilizer once every 7 days.
The compost is applied to fertilizers.
The mass ratio of the peroxidase to the catalase to the pH buffer solution to the livestock and poultry manure is 0.5:1:100: 1000.
Example 7
Use of peroxidase in composting.
Example 8
Use of peroxidase in composting.
The preparation method of the compost comprises the steps of adding peroxidase into livestock and poultry manure containing antibiotics, and stacking for a period of time under a certain condition to obtain the compost without the antibiotics.
Further comprises adding a pH buffer solution; the pH buffer solution is citric acid-sodium hydroxide-hydrochloric acid buffer solution, and the pH is 6.5.
Specifically, peroxidase is dissolved in a pH buffer solution and added to livestock and poultry manure containing antibiotics.
The certain conditions are that the temperature is 18-21 ℃ and the water content is 62-65%; the stacking period is 120 days.
And in the stacking process, turning over the fertilizer once every 4 days.
The compost is applied to fertilizers.
The mass ratio of the peroxidase to the pH buffer solution to the livestock and poultry manure is 6:300: 1000.
The beneficial effects of the present invention are further illustrated below in conjunction with experimental data:
test material
1, materials and methods:
1.1 test site: yongdeng county Wusheng Yizhen black forest village.
1.2 test detection: and detecting the contents of aureomycin, terramycin, tetracycline and doxycycline.
1.3 test materials: CK (before maturation), comparative 1 (except that the peroxidase was replaced by water, the other preparation methods were identical to those of example 1), example 1, example 2, example 3 and example 4.
1.4 Experimental methods:
the dried pig manure was divided into 10 portions, each of 2 groups was used as a parallel sample, and treated with comparative example 1 (except that peroxidase was not replaced with water, and the other preparation methods were identical to those of example 1), example 1, example 2, example 3 and example 4, respectively.
1.5 detection method: GB/T32951-2016.
The experiment was conducted in a consistent manner except for the different experimental treatments.
2 results and analysis
The contents of aureomycin, oxytetracycline, tetracycline and doxycycline in each treatment are shown in Table 1
TABLE 1
Figure GDA0003047631590000051
As can be seen from Table 1, the removal rate of antibiotics can be partially removed by composting, but the removal rate is not very high, the removal rate of antibiotics in the compost can be improved after peroxidase is added, the removal rate of antibiotics in the compost can be further improved by catalase, the removal rate of antibiotics can be further improved by adding a pH buffer solution, and the best effect is achieved by dissolving peroxidase and catalase in the pH buffer solution together.

Claims (5)

1. The application of peroxidase in compost is characterized in that the preparation method of the compost is carried out according to the following steps: dissolving peroxidase and catalase in a pH buffer solution, adding the mixed solution into livestock and poultry manure containing antibiotics, and stacking for a period of time under certain conditions to obtain compost without the antibiotics;
the pH buffer solution is a phosphate buffer solution with the pH of 8.0;
the certain conditions are that the temperature is 22-24 ℃ and the water content is 60-62%; the stacking time is 60 days;
the mass ratio of the peroxidase to the catalase to the pH buffer solution to the livestock and poultry manure is 3:1:399: 1000.
2. The preparation method of the compost is characterized in that peroxidase and catalase are dissolved in a pH buffer solution, then the mixed solution is added into livestock and poultry manure containing antibiotics, and the compost without the antibiotics is obtained after stacking for a period of time under certain conditions;
the pH buffer solution is a phosphate buffer solution with the pH of 8.0;
the certain conditions are that the temperature is 22-24 ℃ and the water content is 60-62%; the stacking time is 60 days;
the mass ratio of the peroxidase to the catalase to the pH buffer solution to the livestock and poultry manure is 3:1:399: 1000.
3. The use of a peroxidase in composting as claimed in claim 1 further comprising turning during stacking.
4. The method of preparing compost of claim 2, further comprising turning the compost during stacking.
5. Use of compost, characterized in that the compost of claim 1 or 2 is applied to manure or farmland.
CN201910572829.1A 2019-06-28 2019-06-28 Application of peroxidase in compost, compost preparation method and application of compost Active CN110143830B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101172745A (en) * 2007-09-21 2008-05-07 宁波工程学院 Livestock and poultry dung or urine deodorizing method and uses thereof
CN102029163A (en) * 2009-09-27 2011-04-27 华中科技大学 Catalase catalyst analog as well as preparation method and application thereof
CN107573121A (en) * 2017-09-21 2018-01-12 宿州天雨养殖（集团）有限公司 A kind of method that fresh chicken manure compost fermentation prepares organic fertilizer
CN109354351A (en) * 2018-10-22 2019-02-19 陈先锐 A kind of method of antibiotic in degradation feces of livestock and poultry

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101172745A (en) * 2007-09-21 2008-05-07 宁波工程学院 Livestock and poultry dung or urine deodorizing method and uses thereof
CN102029163A (en) * 2009-09-27 2011-04-27 华中科技大学 Catalase catalyst analog as well as preparation method and application thereof
CN107573121A (en) * 2017-09-21 2018-01-12 宿州天雨养殖（集团）有限公司 A kind of method that fresh chicken manure compost fermentation prepares organic fertilizer
CN109354351A (en) * 2018-10-22 2019-02-19 陈先锐 A kind of method of antibiotic in degradation feces of livestock and poultry

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Enzymatic degradation of tetracycline and oxytetracycline by crude manganese peroxidase prepared from Phanerochaete chrysosporium;Wen, XH等;《Journal of Hazardous Materials》;20100111;第177卷;第924-928页 *
堆肥消减畜禽粪便中病原微生物及抗生素残留的研究进展;李霞等;《山东农业科学》;20170730;第49卷(第7期);第161-166页 *
木质素降解酶对四环素的降解可行性;贾燕南等;《环境科学学报》;20080115;第28卷(第1期);第68-75页 *

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