CN110143830A - The application of application and composting preparation method and compost of the peroxidase in compost - Google Patents
The application of application and composting preparation method and compost of the peroxidase in compost Download PDFInfo
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- CN110143830A CN110143830A CN201910572829.1A CN201910572829A CN110143830A CN 110143830 A CN110143830 A CN 110143830A CN 201910572829 A CN201910572829 A CN 201910572829A CN 110143830 A CN110143830 A CN 110143830A
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- compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/80—Separation, elimination or disposal of harmful substances during the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Toxicology (AREA)
- Fertilizers (AREA)
Abstract
The application of the application and composting preparation method and compost that the present invention relates to peroxidase in compost, which is characterized in that application of the peroxidase in compost.The removal rate of antibiotic in compost can be improved in the present invention.
Description
Technical field
The application of the application and composting preparation method and compost that the present invention relates to peroxidase in compost.
Background technique
Antibiotic is also known as antibiotic, is the secondary that certain phase generation is grown by microorganisms such as actinomyces, bacterium, fungies
Metabolite directlys adopt substance similar or identical made from chemical synthesis or semi-synthesis method, within the scope of a certain concentration
Have the function of killing or inhibiting pathogen.The antibiotic usage amount of livestock and poultry breeding industry accounts for about the 70% of whole world total output,
And the usage amount of China's annual antimicrobial DP finish in recent years reaches 6000t.Absorption of the antibiotic in enteron aisle is seldom, only on a small quantity
Antibiotic can be absorbed by organisms, in vivo by hydroxylating, cracking and glucoside acidification etc. processes generate it is nontoxic
Substance, about 60~90% can be excreted with original shape with fecaluria, so producing excrement largely containing antibiotic.
Excrement majority is used in farmland, or even is discharged into water body with sewage.Remaining antibiotic is applied soil in feces of livestock and poultry
The ecological balance of Soil Microorganism is not only broken up after earth, and antibiotic can also enter food by being enriched in plant
Chain；Antibiotic in feces of livestock and poultry also can enter water body with approach such as infiltration losses, be contaminated water environment, and to wherein
Biology generate toxic action, in environment remaining antibiotic will Induction of bacterial generate drug resistance or lead to strong drug tolerant bacteria
Generation in addition Induction of bacterial generate and drug resistance gene and shifted in similar bacterium, further harm is generated to human health.
Feces of livestock and poultry, which is directly manured into soil, may cause disease pest and the murder by poisoning to seed and seedling, and usual feces of livestock and poultry exists
Apply farmland before, need to carry out it is decomposed, however, antibiotic have stronger antibacterial, bactericidal property, only with the side of compost maturity
Formula is difficult to be completely removed, and removes antibiotic using compost by the way of at present, and removal rate is 65%~85%, can not allow people
It is satisfied.
Currently, there has been no the methods for further increasing removal antibiotic removal rate.
Summary of the invention
The present invention provides the application of application and composting preparation method and compost of the peroxidase in compost, solves skill
Art problem is the removal rate for further increasing removal antibiotic.
In order to solve the above-mentioned technical problem, the invention adopts the following technical scheme:
Application of the peroxidase in compost.
Application of the peroxidase in compost.
Peroxidase is added in the feces of livestock and poultry containing antibiotic by the preparation method of compost, under certain condition,
To get the compost of removal antibiotic after stacking a period of time.
It further include that catalase is added.
It further include that pH buffer solution is added；The pH buffer solution pH is 6.0~10.
Peroxidase is dissolved in pH buffer solution, is added in the feces of livestock and poultry containing antibiotic；Or
Peroxidase, catalase are dissolved in pH buffer solution, the feces of livestock and poultry containing antibiotic is added to
In.
The certain condition is 5~35 DEG C of temperature, water content 55~75%；Described stacking a period of time is 15~180 days.
It further include carrying out stirring in stacking process.
The mass ratio of peroxidase, pH buffer solution and feces of livestock and poultry is 0.5~15:0~700:1000.
Above-mentioned compost is applied in fertilizer or farmland by the application of compost.
Invention has following advantageous effects:
1. the present invention can further remove antibiotic on the basis of compost.
It, will by the phenol ring and amino in peroxidase deoxidation antibiotic 2. peroxidase is added in the present invention
Antibiotic removal.
3. peroxidase and catalase joint, can preferably remove antibiotic.
4. soda acid buffer solution is added in the application, prevent from locally generating in composting process organic hyper acid so as to cause enzyme
Inactivation；Compound of polysaccharide is hydrolyzed into monosaccharide specially in composting process, monosaccharide resolves into rapidly alcohol, acetic acid, oxalic acid etc., because
This can be such that acid excessive generate to enzyme in part poisons, or even make its inactivation.
5. peroxidase and catalase are added in pH buffer solution by the application, buffer solution can be made more preferable
Enzyme is protected, while being also easy to use.
Specific embodiment
The present invention is further illustrated below with reference to specific example.
Embodiment 1
Application of the peroxidase in compost.
Peroxidase is added in the feces of livestock and poultry containing antibiotic by the preparation method of compost, under certain condition,
To get the compost of removal antibiotic after stacking a period of time.
The certain condition is 22~24 DEG C of temperature, water content 60~62%；Described stacking a period of time is 60 days.
The mass ratio of peroxidase, water and feces of livestock and poultry is 3:400:1000.
Embodiment 2
The application of peroxidase and catalase in compost.
Peroxidase, catalase are added in the feces of livestock and poultry containing antibiotic by the preparation method of compost,
Under certain condition, to get the compost of removal antibiotic after stacking a period of time.
The certain condition is 22~24 DEG C of temperature, water content 60~62%；Described stacking a period of time is 60 days.
Peroxidase, catalase, water and feces of livestock and poultry mass ratio be 3:1:399:1000.
Embodiment 3
The application of peroxidase and catalase in compost.
Peroxidase, catalase and pH buffer solution are added separately to containing antibiosis by the preparation method of compost
In the feces of livestock and poultry of element, under certain condition, to get the compost of removal antibiotic after stacking a period of time.
The pH buffer solution is the phosphate buffer solution that pH is 8.0.
The certain condition is 22~24 DEG C of temperature, water content 60~62%；Described stacking a period of time is 60 days.
Peroxidase, catalase, pH buffer solution and feces of livestock and poultry mass ratio be 3:1:399:1000.
Embodiment 3
The application of peroxidase and catalase in compost.
Peroxidase, catalase are dissolved in pH buffer solution by the preparation method of compost, then by mixed liquor
It is added in the feces of livestock and poultry containing antibiotic, under certain condition, to get the heap of removal antibiotic after stacking a period of time
Fertilizer.
The pH buffer solution is the phosphate buffer solution that pH is 8.0.
The certain condition is 22~24 DEG C of temperature, water content 60~62%；Described stacking a period of time is 60 days.
Peroxidase, catalase, pH buffer solution and feces of livestock and poultry mass ratio be 3:1:399:1000.
Embodiment 4
Application of the peroxidase in compost.
Peroxidase and catalase are dissolved in pH buffer solution by the preparation method of compost, be added to containing
In the feces of livestock and poultry of antibiotic, under certain condition, to get the compost of removal antibiotic after stacking a period of time.
It further include that pH buffer solution is added；The pH buffer solution is Tris- hydrochloric acid buffer solution, pH 8.5.
The certain condition is 26~30 DEG C of temperature, water content 55~60%；Described stacking a period of time is 90 days.
Further include carrying out stirring in stacking process, was turned over every 5 days fertile primary.
Above-mentioned compost is applied in fertilizer.
Peroxidase, catalase, pH buffer solution and feces of livestock and poultry mass ratio be 10:2:600:1000.
Embodiment 5
Application of the peroxidase in compost.
Peroxidase and catalase are added in the feces of livestock and poultry containing antibiotic by the preparation method of compost,
Under certain condition, to get the compost of removal antibiotic after stacking a period of time.
It further include that pH buffer solution is added；The pH buffer solution is Glycine-NaOH buffer solution, pH 9.0.
Specifically, peroxidase and catalase are dissolved in pH buffer solution, it is added to containing antibiotic
In feces of livestock and poultry.
The certain condition is 15~20 DEG C of temperature, water content 60~65%；Described stacking a period of time is 120 days.
Further include carrying out stirring in stacking process, was turned over every 7 days fertile primary.
Above-mentioned compost is applied in fertilizer.
Peroxidase, catalase, pH buffer solution and feces of livestock and poultry mass ratio be 0.5:1:100:1000.
Embodiment 6
Application of the peroxidase in compost.
Embodiment 7
Application of the peroxidase in compost.
Peroxidase is added in the feces of livestock and poultry containing antibiotic by the preparation method of compost, under certain condition,
To get the compost of removal antibiotic after stacking a period of time.
It further include that pH buffer solution is added；The pH buffer solution is citric acid-sodium hydroxide-hydrochloric acid buffer solution, pH
It is 6.5.
Specifically, peroxidase is dissolved in pH buffer solution, it is added in the feces of livestock and poultry containing antibiotic.
The certain condition is 18~21 DEG C of temperature, water content 62~65%；Described stacking a period of time is 120 days.
Further include carrying out stirring in stacking process, was turned over every 4 days fertile primary.
Above-mentioned compost is applied in fertilizer.
The mass ratio of peroxidase, pH buffer solution and feces of livestock and poultry is 6:300:1000.
Beneficial effects of the present invention are further illustrated below with reference to experimental data:
Material to be tested
1 materials and methods:
1.1 test sites: the Yongdeng County Wusheng post town village Hei Lin.
1.2 experiment detections: the content of detection aureomycin, terramycin, tetracycline and fortimicin.
1.3 materials to be tested: CK (before not decomposed), comparison 1 (except non-peroxidase by water substitution in addition to, other preparation methods
Unanimously with embodiment 1), embodiment 1, embodiment 2, embodiment 3 and embodiment 4.
1.4 experimental methods:
The pig manure dried is divided into 10 parts, every 2 groups are Duplicate Samples, and comparison 1 is respectively adopted (except non-peroxidase is replaced by water
In generation, is outer, and other preparation methods are consistent with embodiment 1), embodiment 1, embodiment 2, embodiment 3 and embodiment 4 handled.
1.5 detection methods: GB/T 32951-2016.
In addition to experiment process is different, other operations are consistent for this experiment.
2 results and analysis
Aureomycin, terramycin, tetracycline and fortimicin content are shown in Table 1 in each processing
Table 1
As can be seen from Table 1, part antibiotic can be removed by composting mode, but removal rate is not very high, and added
After entering peroxidase, the removal rate of antibiotic in compost can be improved, catalase is added can be further improved again
The removal rate of antibiotic in compost, pH buffer solution, which is added, can be further improved the removal rate of removal antibiotic, by peroxidating
Object enzyme and catalase are dissolved in pH buffer solution that effect is added together is best.
Claims (9)
1. application of the peroxidase in compost.
2. the preparation method of compost, which is characterized in that peroxidase is added in the feces of livestock and poultry containing antibiotic, one
Under fixed condition, to get the compost of removal antibiotic after stacking a period of time.
3. the preparation method of compost as claimed in claim 2, which is characterized in that further include that catalase is added.
4. the preparation method of compost as described in Claims 2 or 3, which is characterized in that further include that pH buffer solution is added；The pH
Buffer solution pH is 6.0~10.
5. the preparation method of compost as claimed in claim 4, which is characterized in that peroxidase is dissolved in pH buffer solution
In, it is added in the feces of livestock and poultry containing antibiotic；Or
Peroxidase, catalase are dissolved in pH buffer solution, are added in the feces of livestock and poultry containing antibiotic.
6. the preparation method of compost as described in claim 2 to 5 any one, which is characterized in that the certain condition is temperature 5
~35 DEG C, water content 55~75%；Described stacking a period of time is 15~180 days.
7. the preparation method of compost as claimed in claim 6, which is characterized in that further include carrying out stirring in stacking process.
8. the preparation method of compost as claimed in claim 5, which is characterized in that peroxidase, pH buffer solution and animal dung
Just mass ratio is 0.5~15:0~700:1000.
9. the application of compost, which is characterized in that above-mentioned compost to be applied in fertilizer or farmland.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910572829.1A CN110143830B (en) | 2019-06-28 | 2019-06-28 | Application of peroxidase in compost, compost preparation method and application of compost |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910572829.1A CN110143830B (en) | 2019-06-28 | 2019-06-28 | Application of peroxidase in compost, compost preparation method and application of compost |
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| Publication Number | Publication Date |
|---|---|
| CN110143830A true CN110143830A (en) | 2019-08-20 |
| CN110143830B CN110143830B (en) | 2021-09-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910572829.1A Active CN110143830B (en) | 2019-06-28 | 2019-06-28 | Application of peroxidase in compost, compost preparation method and application of compost |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101172745A (en) * | 2007-09-21 | 2008-05-07 | 宁波工程学院 | Livestock and poultry dung or urine deodorizing method and uses thereof |
| CN102029163A (en) * | 2009-09-27 | 2011-04-27 | 华中科技大学 | Catalase catalyst analog as well as preparation method and application thereof |
| CN107573121A (en) * | 2017-09-21 | 2018-01-12 | 宿州天雨养殖（集团）有限公司 | A kind of method that fresh chicken manure compost fermentation prepares organic fertilizer |
| CN109354351A (en) * | 2018-10-22 | 2019-02-19 | 陈先锐 | A kind of method of antibiotic in degradation feces of livestock and poultry |
-
2019
- 2019-06-28 CN CN201910572829.1A patent/CN110143830B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101172745A (en) * | 2007-09-21 | 2008-05-07 | 宁波工程学院 | Livestock and poultry dung or urine deodorizing method and uses thereof |
| CN102029163A (en) * | 2009-09-27 | 2011-04-27 | 华中科技大学 | Catalase catalyst analog as well as preparation method and application thereof |
| CN107573121A (en) * | 2017-09-21 | 2018-01-12 | 宿州天雨养殖（集团）有限公司 | A kind of method that fresh chicken manure compost fermentation prepares organic fertilizer |
| CN109354351A (en) * | 2018-10-22 | 2019-02-19 | 陈先锐 | A kind of method of antibiotic in degradation feces of livestock and poultry |
Non-Patent Citations (4)
| Title |
|---|
| WEN, XH等: "Enzymatic degradation of tetracycline and oxytetracycline by crude manganese peroxidase prepared from Phanerochaete chrysosporium", 《JOURNAL OF HAZARDOUS MATERIALS》 * |
| 李霞等: "堆肥消减畜禽粪便中病原微生物及抗生素残留的研究进展", 《山东农业科学》 * |
| 贾燕南等: "木质素降解酶对四环素的降解可行性", 《环境科学学报》 * |
| 钟洪枢等: "《生物化学》", 31 October 1988, 高等教育出版社 * |
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Inventor after: Dong Bo Inventor after: Jiang Xiaofeng Inventor after: Zeng Jun Inventor after: Liu Xiaowei Inventor after: Zhang Yingying Inventor after: Tian Jianxia Inventor after: Sun Pengzhou Inventor after: Zhang Guoping Inventor after: Wang Lei Inventor after: Song Shuzhen Inventor after: Ding Yang Inventor after: Zhang Rui Inventor after: Chen Guangrong Inventor after: Gan Yandong Inventor after: Jiang Jing Inventor before: Dong Bo Inventor before: Jiang Xiaofeng Inventor before: Zeng Jun Inventor before: Liu Xiaowei Inventor before: Chen Guangrong Inventor before: Song Shuzhen |