CN110117208A - Ecological fertilizer and preparation method thereof - Google Patents
Ecological fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN110117208A CN110117208A CN201910578508.2A CN201910578508A CN110117208A CN 110117208 A CN110117208 A CN 110117208A CN 201910578508 A CN201910578508 A CN 201910578508A CN 110117208 A CN110117208 A CN 110117208A
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- Prior art keywords
- fertilizer
- peroxidase
- buffer solution
- catalase
- ecological
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
Abstract
The present invention relates to ecological fertilizers and preparation method thereof, which is characterized in that is made of fertilizer and peroxidase.The present invention can degrade antibiotic, safeguard the balance of edaphon, meanwhile, the application is applied in hardened soil, can provide oxygen for root system, store nutriment, achieve the purpose that volume increase；Oxygen is provided for soil simultaneously, inhibits the activity of anaerobic bacteria, improves utilization rate of fertilizer.
Description
Technical field
The present invention relates to ecological fertilizers and preparation method thereof.
Background technique
Antibiotic is also known as antibiotic, is the secondary that certain phase generation is grown by microorganisms such as actinomyces, bacterium, fungies
Metabolite directlys adopt substance similar or identical made from chemical synthesis or semi-synthesis method, within the scope of a certain concentration
Have the function of killing or inhibiting pathogen.The antibiotic usage amount of livestock and poultry breeding industry accounts for about the 70% of whole world total output, and
The usage amount of China's annual antimicrobial DP finish in recent years reaches 6000t.Absorption of the antibiotic in enteron aisle is seldom, only a small amount of
Antibiotic can be absorbed by organisms, and generate nontoxic object by processes such as hydroxylating, cracking and glucoside acidifications in vivo
Matter, about 60~90% can be excreted with original shape with fecaluria, so producing excrement largely containing antibiotic.
Feces of livestock and poultry containing antibiotic, which is directly manured into soil, may cause disease pest and the murder by poisoning to seed and seedling, lead to
Normal feces of livestock and poultry before applying farmland, need to carry out it is decomposed, however, antibiotic is with stronger antibacterial, bactericidal property, only with
The mode of compost maturity is difficult to be completely removed, and removes antibiotic by the way of compost at present, and removal rate is 65%~85%.
Therefore, a large amount of antibiotic still is flowed into farmland.
The antibiotic being manured into soil with feces of livestock and poultry can be adsorbed by soil particle, and can long-term accumulation, when in soil
Antibiotic when running up to a certain amount of, just will affect fungi in soil, bacterium and actinomyces, not only influence microorganism mushroom group
Structure, can also inducing microbial generate drug resistance, inhibit or enhancing microorganism metabolic pathway in addition antibiotic can inhibit or
Person directly kills microorganism, destroys the Tiny ecosystem structure in soil, and then the rotten and decomposition for influencing organic matter keeps soil fertile
Power decline.
Long-term unreasonable fertilising, causes soil fertility to decline, and soil acidification is hardened serious, and then affects root system of plant
Respiration, destroy " Pasteur effect ", cause glycolysis rate accelerate, prevent nutriment from storing in plant
It deposits, has in turn resulted in the underproduction.Meanwhile soil hardening causes soil porosity to reduce, permeability is poor, and ground temperature reduces, and causes soil
The activity of middle aerobic microorganism is suppressed, and utilization rate of fertilizer reduces, and has also delayed the decomposition of organic matter, soil physico-chemical property
It runs down, soil fertility gradually fails, and soil fertility declines therewith, cannot meet plant growth and development well.
It, can not using the method degradation antibiotic that microorganism is added since antibiotic has very strong antibacterial, sterilizing ability
Reach satisfactory effect, there has been no the methods of antibiotic in better degradation soil at present；Also there is not degradation antibiotic
Meanwhile root system of plant to be acidified in hardened soil and aerobic microbiological provide oxygen, maintain " Pasteur effect ", prevent crop cut
It produces, maintains the activity of aerobe, improve utilization rate of fertilizer.
Summary of the invention
The present invention provides ecological fertilizer and preparation method thereof, and solving technical problem is 1) the better antibiosis in degradation soil
Element；2) root system of plant to be acidified in hardened soil provides oxygen, maintains " Pasteur effect ", prevents crop failure；3) it maintains
Gas microbial activity improves utilization rate of fertilizer.
In order to solve the above-mentioned technical problem, the invention adopts the following technical scheme:
Ecological fertilizer is made of fertilizer and peroxidase.
Moderate-element and microelement are Chelating state in the fertilizer；Contain organic matter, the organic matter in the fertilizer
It can be by peroxidase oxidization.
It further include catalase.
It further include pH buffer solution.
Peroxidase, catalase and pH buffer solution are added in fertilizer, i.e., by the preparation method of ecological fertilizer
Obtain ecological fertilizer.
Peroxidase and catalase are added in pH buffer solution and dissolved, mixed liquor is obtained, mixed liquor is added
Into fertilizer, Eco-fertilizer is obtained.
Oxide enzyme, catalase, pH buffer solution and fertilizer mass ratio be 0.5~15:0~15:0~700:
1000。
Invention has following advantageous effects:
1. peroxidase is added in the present invention, by the phenol ring and amino in peroxidase deoxidation antibiotic, further
Antibiotic is removed, prevents antibiotic from assembling in the soil, destroys microecological balance.
2. microelement in Chelating state is added in the application, prevent in inorganic states microelement to peroxidase and mistake
Hydrogen oxide enzyme is poisoned, and is caused the inactivation of enzyme, is influenced the effect of product.
3. soda acid buffer solution is added in the application, the effect for preventing enzyme from inactivating is played, root system of plant divides when absorbing nutrition
Secrete organic acid, buffer solution prevent root system of plant it is locally organic hyper acid causes the inactivation of enzyme, influence the effect of product.
4. peroxidase is added in pH buffer solution by the application, buffer solution can be made preferably to peroxide
Enzyme is protected, while being also easy to use.
5. the application can be improved utilization rate of fertilizer, yield is improved, specific reaction is that substrate oxidation is by peroxidase
Hydrogen peroxide, RH2+O2→R+H2O2,CatalaseIt can use hydrogen peroxide again, by other substrates (such as aldehyde, alcohol, phenol) oxygen
Change, R ' H2+H2O2→R′+2H2O, while catalase can also be catalyzed following reaction: 2H2O2 → 2H2O + O2.Fertilizer benefit
With the low reason of rate: in being acidified hardened soil, soil is often in anaerobic condition, and anaerobic bacteria can convert nitrate nitrogen at this time
For nitrogen, the waste of nitrogen is caused；Simultaneously under conditions of anaerobism, H2SO4 can be reduced into H2S by sulfatereducting bacteria, and H2S is micro- in
Secondary element, which combines, generates MS(precipitating), to reduce the utilization rate of middle microelement.
Specific embodiment
The present invention is further illustrated below with reference to specific example.
Embodiment 1
Ecological fertilizer is made of fertilizer and peroxidase.
The fertilizer is the compound fertilizer that roller is granulated；Raw material of mix-compound fertilizer be urea, monoammonium phosphate, organic matter, bentonite and
EDTAZn according to mass ratio 4:3:2:0.9:0.1 composition；In the fertilizer organic matter be potassium fulvate and farmyard manure according to
The composition of mass ratio 1:1.
Peroxidase is added in the feces of livestock and poultry containing antibiotic, in certain condition by the preparation method of farmyard manure
Under, to get farmyard manure after stacking a period of time.
The certain condition is 22~24 DEG C of temperature, water content 60~62%；Described stacking a period of time is 60 days.
The mass ratio of peroxidase, water and feces of livestock and poultry is 3:400:1000.
Embodiment 2
Ecological fertilizer is made of fertilizer, peroxidase and catalase.
The fertilizer is the compound fertilizer that roller is granulated；Raw material of mix-compound fertilizer be urea, monoammonium phosphate, organic matter, bentonite and
EDTAZn according to mass ratio 4:3:2:0.9:0.1 composition；In the fertilizer organic matter be potassium fulvate and farmyard manure according to
The composition of mass ratio 1:1.
Peroxidase, catalase are added in the feces of livestock and poultry containing antibiotic by the preparation method of farmyard manure,
Under certain condition, to get farmyard manure after stacking a period of time.
The certain condition is 22~24 DEG C of temperature, water content 60~62%；Described stacking a period of time is 60 days.
Peroxidase, catalase, water and feces of livestock and poultry mass ratio be 3:1:399:1000.
Embodiment 3
Ecological fertilizer is made of fertilizer, peroxidase and catalase.
The fertilizer is the compound fertilizer that roller is granulated；Raw material of mix-compound fertilizer be urea, monoammonium phosphate, organic matter, bentonite and
EDTAZn according to mass ratio 4:3:2:0.9:0.1 composition；In the fertilizer organic matter be potassium fulvate and farmyard manure according to
The composition of mass ratio 1:1.
Peroxidase, catalase and pH buffer solution are added separately to containing anti-by the preparation method of farmyard manure
In the feces of livestock and poultry of raw element, under certain condition, to get the compost of removal antibiotic after stacking a period of time.
The pH buffer solution is the phosphate buffer solution that pH is 8.0.
The certain condition is 22~24 DEG C of temperature, water content 60~62%；Described stacking a period of time is 60 days.
Peroxidase, catalase, pH buffer solution and feces of livestock and poultry mass ratio be 3:1:399:1000.
Embodiment 4
Ecological fertilizer is made of fertilizer, peroxidase and catalase.
The fertilizer is the compound fertilizer that roller is granulated；Raw material of mix-compound fertilizer be urea, monoammonium phosphate, organic matter, bentonite and
EDTAZn according to mass ratio 4:3:2:0.9:0.1 composition；In the fertilizer organic matter be potassium fulvate and farmyard manure according to
The composition of mass ratio 1:1.
Peroxidase, catalase are dissolved in pH buffer solution by the preparation method of farmers', then by mixed liquor
It is added in the feces of livestock and poultry containing antibiotic, under certain condition, to get farmyard manure after stacking a period of time.
The pH buffer solution is the phosphate buffer solution that pH is 8.0.
The certain condition is 22~24 DEG C of temperature, water content 60~62%；Described stacking a period of time is 60 days.
Peroxidase, catalase, pH buffer solution and feces of livestock and poultry mass ratio be 3:1:399:1000.
Embodiment 5
Ecological fertilizer is made of fertilizer, peroxidase and pH buffer solution.
The fertilizer is organic and inorganic fertilizer；Raw material is ammonium sulfate, monoammonium phosphate, potassium sulfate, bentonite, plant ash, yellow corruption
Sour potassium and humic acid according to mass ratio 3:2:1:0.8:0.1:1:2 composition.
Peroxidase is dissolved in pH buffer solution, obtains mixed liquor by the preparation method of ecological fertilizer, by mixed liquor plus
Enter into fertilizer to get ecological fertilizer.
The mass ratio of peroxidase, pH buffer solution and fertilizer is 1:60:1000.
The pH buffer solution is Tris- hydrochloric acid buffer solution, pH 8.5.
Embodiment 6
Ecological fertilizer is made of fertilizer, peroxidase, catalase and pH buffer solution.
The fertilizer is organic and inorganic fertilizer；Raw material is ammonium sulfate, monoammonium phosphate, potassium sulfate, bentonite, plant ash, yellow corruption
Sour potassium and humic acid according to mass ratio 3:2:1:0.8:0.1:1:2 composition.
Peroxidase, catalase are dissolved in pH buffer solution by the preparation method of ecological fertilizer, must be mixed
Mixed liquor is added in fertilizer to get ecological fertilizer by liquid.
Peroxidase, catalase, pH buffer solution and fertilizer mass ratio be 1:1:60:1000.
The pH buffer solution is Tris- hydrochloric acid buffer solution, pH 8.5.
Embodiment 7
Ecological fertilizer is made of liquid fertilizer and peroxidase.
The liquid fertilizer group is as 20 parts of urea, 6 parts of potassium dihydrogen phosphate, EDTACa1 parts, 5 parts of algae essence and 68 parts of water
Composition.
It further include catalase.
It further include pH buffer solution.The pH buffer solution is citric acid-sodium hydroxide-hydrochloric acid buffer solution, and pH is
6.5。
Peroxidase and catalase are added in pH buffer solution and dissolved, mixed liquor is obtained, mixed liquor is added
Into fertilizer, Eco-fertilizer is obtained.
Peroxidase, catalase, pH buffer solution and liquid fertilizer mass ratio be 5:2:100:1000.
Embodiment 8
Ecological fertilizer is made of fertilizer and peroxidase.
The fertilizer is biological organic fertilizer；By potassium humate, yeast powder, mushroom residue, ammonium sulfate, potassium sulfate, attapulgite
It is formed with bacillus subtilis according to mass ratio 100:50:100:300:200:249:1.
It further include catalase.
It further include pH buffer solution.The pH buffer solution is citric acid-sodium hydroxide-hydrochloric acid buffer solution, and pH is
6.5。
Peroxidase, catalase and pH buffer solution are added in fertilizer to get ecological fertilizer.
Oxide enzyme, catalase, pH buffer solution and fertilizer mass ratio be 8:3:500:1000.
Beneficial effects of the present invention are further illustrated below with reference to experimental data:
Material to be tested
1 materials and methods:
1.1 test sites: the Yongdeng County Wusheng post town village Hei Lin.
1.2 experiment detections: the content of detection aureomycin, terramycin, tetracycline and fortimicin.
1.3 materials to be tested: CK (before not decomposed), comparison 1(are in addition to non-peroxidase is by water substitution, other preparation methods
It is consistent with the preparation method of farmyard manure in embodiment 1), embodiment 1 prepare farmyard manure, embodiment 2 prepare farmyard manure, reality
Farmyard manure prepared by the farmyard manure and embodiment 4 for applying the preparation of example 3.
1.4 experimental methods:
The pig manure dried is divided into 10 parts, every 2 groups are Duplicate Samples, and comparison 1(is respectively adopted except non-peroxidase is substituted by water
Outside, other preparation methods are consistent with embodiment 1), embodiment 1, embodiment 2, embodiment 3 and embodiment 4 handled.
1.5 detection methods: GB/T 32951-2016.
In addition to experiment process is different, other operations are consistent for this experiment.
2 results and analysis
Aureomycin, terramycin, tetracycline and fortimicin content are shown in Table 1 in each processing
Table 1
| Aureomycin (mg/Kg) | Terramycin (mg/Kg) | Tetracycline (mg/Kg) | Fortimicin (mg/Kg) | Removal rate (%) | |
| CK | 29.26 | 36.52 | 39.67 | 4.23 | |
| Comparison 1 | 9.56 | 11.35 | 12.41 | 1.75 | 68.02 |
| Embodiment 1 | 7.43 | 10.36 | 11.24 | 1.63 | 72.05 |
| Embodiment 2 | 5.98 | 7.75 | 8.85 | 1.57 | 77.98 |
| Embodiment 3 | 5.52 | 6.95 | 7.78 | 1.46 | 80.21 |
| Embodiment 4 | 3.21 | 5.27 | 5.66 | 1.24 | 85.98 |
As can be seen from Table 1, after peroxidase being added, the removal rate of antibiotic in compost, catalase can be improved
The removal rate that can be further improved antibiotic in compost again is added, pH buffer solution, which is added, can be further improved removal antibiosis
Peroxidase and catalase, are dissolved in pH buffer solution that effect is added together is best by the removal rate of element.
It can be seen that peroxidase and catalase have the effect of removing antibiotic, and the two is used in combination
Effect is more preferable.
Experiment 2
1.1 test sites: the Yongdeng County Wusheng post town village Hei Lin.
1.2 experiment detections: in corn yield and corn stover and seed in the content of crude protein and every 100g seed
Calcium and content of magnesium.
1.3 materials to be tested: comparison 1(in addition to peroxidase is not added when farmyard manure is decomposed, other preparation methods with reality
It is consistent to apply example 1), embodiment 1, embodiment 2, embodiment 3 and embodiment 4.
1.4 experimental methods:
Experimental plot takes the close 10 mu of adjacent fields of ground condition, and Tanaka's soil is hardened soil, and gas permeability is 7.0~7.2%, with every two
Mu is one group.Seeding corn kind 2.3kg per acre, using mechanical planting, maize seed is grand flat 206, and every group is respectively adopted comparison 1
(in addition to peroxidase is not added when farmyard manure is decomposed, other preparation methods are consistent with embodiment 1), embodiment 1, embodiment
2, embodiment 3 and embodiment 4 are used as base fertilizer, and base applies 100kg/ mus.
1.5 detection methods: Kjeldahl's method detects the percentage composition (N%) of nitrogen in stalk and seed, crude protein content
(CPC%)=N%*6.25.
In addition to experiment process is different, other operations are consistent for this experiment.
2 results and analysis
Corn yield and corn kernel and straw crude protein content, calcium and content of magnesium are shown in Table 2 in every 100g seed
Table 2
| Yield (kg) | Seed crude protein content (%) | Straw crude protein (%) | Calcium (mg) | Magnesium (mg) | |
| Comparison 1 | 657.6 | 8.31 | 5.46 | 2.2 | 9.6 |
| Embodiment 1 | 659.3 | 8.32 | 5.47 | 2.2 | 9.7 |
| Embodiment 2 | 683.5 | 8.39 | 5.54 | 2.7 | 10.2 |
| Embodiment 3 | 697.2 | 8.57 | 5.61 | 2.7 | 10.3 |
| Embodiment | 712.5 | 9.32 | 5.89 | 3.5 | 10.6 |
As can be seen from Table 2, yield and crude protein content can be slightly improved after peroxidase being added, but effect is unknown
It is aobvious, and the embodiment 2 that peroxidase and catalase is added then is promoted more obvious, is catalase by peroxidating
The H2O2 that object oxydasis generates is decomposed into H2O and O2, provides oxygen to be acidified in hardened soil, to prevent " Pasteur effect
Answer " it is destroyed, so that plant physical efficiency is assembled more nutriments, achievees the purpose that volume increase；Meanwhile oxygen can guarantee again it is aerobic
The living environment of bacterium, reduces the generation of anti-nitration reaction, to reduce the waste of nitrogen, reduces sulfate reduction reaction, prevent in it is micro-
Secondary element is fixed, to improve utilization rate of fertilizer.
Claims (8)
1. ecological fertilizer, which is characterized in that be made of fertilizer and peroxidase.
2. ecological fertilizer as described in claim 1, which is characterized in that moderate-element and microelement are chelating in the fertilizer
State；Contain organic matter in the fertilizer, the organic matter can be by peroxidase oxidization.
3. ecological fertilizer as claimed in claim 1 or 2, which is characterized in that further include catalase.
4. ecological fertilizer as claimed in claim 1 or 2, which is characterized in that further include pH buffer solution.
5. ecological fertilizer as claimed in claim 3, which is characterized in that further include pH buffer solution.
6. the preparation method of ecological fertilizer, which is characterized in that peroxidase, catalase and pH buffer solution to be added to
To get ecological fertilizer in fertilizer.
7. the preparation method of ecological fertilizer as claimed in claim 6, which is characterized in that add peroxidase and catalase
Enter into pH buffer solution and dissolve, obtain mixed liquor, mixed liquor is added in fertilizer, obtains Eco-fertilizer.
8. the preparation method of ecological fertilizer as claimed in claims 6 or 7, which is characterized in that oxide enzyme, catalase, pH
The mass ratio of buffer solution and fertilizer is 0.5~15:0~15:0~700:1000.
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| CN201910578508.2A CN110117208A (en) | 2019-06-28 | 2019-06-28 | Ecological fertilizer and preparation method thereof |
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| CN201910578508.2A CN110117208A (en) | 2019-06-28 | 2019-06-28 | Ecological fertilizer and preparation method thereof |
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ID=67524447
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111848288A (en) * | 2020-08-08 | 2020-10-30 | 山东喜美农业科技有限公司 | Water-soluble fertilizer and preparation method thereof |
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| CN102029163A (en) * | 2009-09-27 | 2011-04-27 | 华中科技大学 | Catalase catalyst analog as well as preparation method and application thereof |
| US20140256547A1 (en) * | 2008-01-15 | 2014-09-11 | Board Of Trustees Of Michigan State University | Polymicrobial formulations for enhancing plant productivity |
| CN107266192A (en) * | 2017-07-12 | 2017-10-20 | 烟台市昊星晟信息技术有限公司 | A kind of production method for the ecological organic fertilier for removing antibiotic |
| CN107573121A (en) * | 2017-09-21 | 2018-01-12 | 宿州天雨养殖（集团）有限公司 | A kind of method that fresh chicken manure compost fermentation prepares organic fertilizer |
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2019
- 2019-06-28 CN CN201910578508.2A patent/CN110117208A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140256547A1 (en) * | 2008-01-15 | 2014-09-11 | Board Of Trustees Of Michigan State University | Polymicrobial formulations for enhancing plant productivity |
| CN102029163A (en) * | 2009-09-27 | 2011-04-27 | 华中科技大学 | Catalase catalyst analog as well as preparation method and application thereof |
| CN107266192A (en) * | 2017-07-12 | 2017-10-20 | 烟台市昊星晟信息技术有限公司 | A kind of production method for the ecological organic fertilier for removing antibiotic |
| CN107573121A (en) * | 2017-09-21 | 2018-01-12 | 宿州天雨养殖（集团）有限公司 | A kind of method that fresh chicken manure compost fermentation prepares organic fertilizer |
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| CN111848288A (en) * | 2020-08-08 | 2020-10-30 | 山东喜美农业科技有限公司 | Water-soluble fertilizer and preparation method thereof |
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Application publication date: 20190813 |