CN110133144A - Nucleic acid-protein detection device and method based on LED light source - Google Patents
Nucleic acid-protein detection device and method based on LED light source Download PDFInfo
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- CN110133144A CN110133144A CN201910444790.5A CN201910444790A CN110133144A CN 110133144 A CN110133144 A CN 110133144A CN 201910444790 A CN201910444790 A CN 201910444790A CN 110133144 A CN110133144 A CN 110133144A
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- 238000002331 protein detection Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title description 8
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 21
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 21
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 21
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 20
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 20
- 230000003321 amplification Effects 0.000 claims abstract description 17
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 17
- 238000006243 chemical reaction Methods 0.000 claims abstract description 14
- 239000000835 fiber Substances 0.000 claims abstract description 14
- 230000005284 excitation Effects 0.000 claims abstract description 12
- 230000008878 coupling Effects 0.000 claims abstract description 4
- 238000010168 coupling process Methods 0.000 claims abstract description 4
- 238000005859 coupling reaction Methods 0.000 claims abstract description 4
- 238000001514 detection method Methods 0.000 claims description 21
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- 238000002834 transmittance Methods 0.000 claims description 16
- 238000004458 analytical method Methods 0.000 claims description 10
- 238000001228 spectrum Methods 0.000 claims description 9
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- 238000004321 preservation Methods 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 5
- 238000004587 chromatography analysis Methods 0.000 claims description 5
- 230000005622 photoelectricity Effects 0.000 claims description 5
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- 230000003287 optical effect Effects 0.000 claims 1
- 238000012360 testing method Methods 0.000 description 6
- 239000011521 glass Substances 0.000 description 4
- 238000010828 elution Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
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- 238000005259 measurement Methods 0.000 description 2
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 2
- 229910052753 mercury Inorganic materials 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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- 230000003595 spectral effect Effects 0.000 description 2
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- 229910052724 xenon Inorganic materials 0.000 description 2
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- 241000258971 Brachiopoda Species 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/64—Electrical detectors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/64—Electrical detectors
- G01N2030/645—Electrical detectors electrical conductivity detectors
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
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- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8827—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving nucleic acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
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Abstract
The nucleic acid-protein detection device based on LED light source that the invention discloses a kind of, embedded acquisition controller, which is electrically connected and controls ultra-violet light-emitting module, issues ultraviolet source, the ultraviolet source that ultra-violet light-emitting module issues is irradiated into sample cell through fiber coupler, ultraviolet light receives ultraviolet transmissive light by ultraviolet light photo sensor after sample cell, and ultraviolet light photo sensor is sequentially connected electrically small-signal amplification module and logarithmic converter；Conductivity sensor is installed in sample cell, conductivity sensor is sequentially connected electrically excitation conversion module, amplifier module and phase-sensitive detector, the logarithmic converter and phase-sensitive detector pass through A/D converter and are electrically connected embedded acquisition controller, and embedded acquisition controller is electrically connected nucleic acid protein chromatographic work station by interface controller.Light source is irradiated into sample cell by fiber coupling and beam path alignment system using ultra-violet light-emitting chip as light source (LED) by the present invention, receives ultraviolet transmissive luminous intensity with photoelectric sensor.
Description
Technical field
The invention belongs to technological field of biochemistry, in particular to a kind of nucleic acid-protein detection device based on LED light source
And method.
Background technique
During bioscience and bio-pharmaceuticals are researched and produced, nucleic acid-protein detection device is non-in chromatographic assay system
Often important detection device.Mix chromatographic column, constant flow pump, part collector (apolegamy as needed) and computerized print equipment i.e. structure
At the liquid chromatographic separation system of complete set.It has become and is engaged in life science, drug monitoring, chemical industry, environmental protection, food now
The modern analysis laboratory apparatus of the industries such as product science and medical research.It is widely used in the section of industry, agricultural, scientific research and universities and colleges
Learn research and teaching experiment.Its principle is that had to ultraviolet certain wave with the feature obviously absorbed according to substance (sample)
Effect is collected, and is compared and is analyzed to sample component content, to carry out the material identifications such as sample protein, nucleic acid and assay.
Although the nucleic acid-protein detector of current domestic production is many kinds of, there are obvious problems:
1, the ultraviolet source used is all made of mercury lamp and xenon source.Mercury lamp shell is glass and easily broken, mercuryvapour volatilization tool
There is very big security risk, national requirements for environmental protection cannot be met；Xenon lamp housing is also glass and easily broken, and calorific value is big when work,
Service life is shorter.
2, photoelectric conversion uses expensive import photomultiplier tube.Photomultiplier tube works in high pressure conditions, glass
Glass shell, transportational process is easily broken, and preheating time is very long.Therefore, ultraviolet nucleic acid-protein detection field is badly in need of Environmental Safety, firm
Efficient new ultra-violet nucleic acid-protein detection device.
Summary of the invention
In order to solve the problems in the prior art, the present invention provides a kind of, and the nucleic acid-protein based on LED light source detects dress
It sets and method.
To achieve the above object, the technical solution adopted by the present invention are as follows:
A kind of nucleic acid-protein detection device based on LED light source, detection device include ultra-violet light-emitting module, photoelectricity collimation reception dress
It sets, small-signal amplification module, conductivity sensor, excitation conversion module, Conductivity detection amplification module, logarithmic converter, phase sensitivity
Wave detector, A/D converter, embedded acquisition controller, interface controller and chromatography work station, the photoelectricity collimate reception device
Including fiber coupler, sample cell and ultraviolet light transducer, in which:
Embedded acquisition controller, which is electrically connected and controls ultra-violet light-emitting module, issues ultraviolet source, the purple that ultra-violet light-emitting module issues
Outer light source is irradiated into sample cell through fiber coupler, and it is purple that ultraviolet light receives transmission by ultraviolet light photo sensor after sample cell
Outer light, ultraviolet light photo sensor are sequentially connected electrically small-signal amplification module and logarithmic converter；Installation conductance passes in sample cell
Sensor, conductivity sensor are sequentially connected electrically excitation conversion module, amplifier module and phase-sensitive detector, the logarithmic converter and
Phase-sensitive detector passes through A/D converter and is electrically connected embedded acquisition controller, and embedded acquisition controller passes through Interface Controller
Device is electrically connected nucleic acid protein chromatographic work station；
The signal of the ultraviolet light photo sensor successively obtains sample cell liquid through small-signal amplification module and logarithmic converter
To the absorption signal of ultraviolet light；The signal of conductivity sensor is successively through excitation conversion module, amplifier module and phase-sensitive detector
Obtain the conductance signal (ionic strength) of sample cell liquid；The absorption signal and conductance signal are transferred to embedding through A/D converter
Enter formula acquisition controller, embedded acquisition controller obtains uv transmittance and conductivity, and embedded acquisition controller passes through
Uv transmittance and conductivity are transferred to nucleic acid protein chromatographic work station by interface controller；
The nucleic acid protein chromatographic work station carries out data receiver, screen draws spectrum, Parameter analysis, spectrogram editor, data preservation work
Make.
Preferably, the characteristic wavelength for the ultraviolet source that the ultra-violet light-emitting module issues be respectively 260nm light source and
The light source of 280nm.
Further, the ultraviolet source that ultra-violet light-emitting module issues is sent to sample cell through optical fiber by the fiber coupler.
Preferably, the capacity of the sample cell is 70ul, light path 4mm.
Preferably, the ultraviolet light transducer model can be GUVB-T11GD etc..
Preferably, the MCU in the embedded acquisition controller is ARM, model STM32F103 series.
Further, the uv transmittance of liquid and conductivity data work in nucleic acid protein chromatographic in the sample cell
Real-time display on the screen stood.
A kind of detection method of the nucleic acid-protein detection device based on LED light source, treated that sample is placed in sample for chromatographic column
In product pond, the sample flowed out in sample cell is collected by part collector；
Embedded acquisition controller control ultra-violet light-emitting module issues a branch of or two beam ultraviolet sources, and ultraviolet source is through fiber coupling
Device is irradiated into sample cell, and ultraviolet source is through sample cell and transmission enhancement is in ultraviolet light photo sensor, and ultraviolet light photo sensor obtains
With the directly proportional absorption signal of transmitted intensity, absorption signal successively through small-signal amplification module and logarithmic converter, this
When obtained absorption signal intensity and sample cell absorbance it is in direct ratio, absorption signal is converted into number through A/D converter again
Signal is transferred to embedded acquisition controller, and embedded acquisition controller obtains the uv transmittance of liquid in sample cell；Together
When, the conductance signal that the interior conductivity sensor being arranged of sample cell will obtain, conductance signal is successively examined through excitation conversion module, conductance
Amplification module and phase-sensitive detector are surveyed, obtains the conductance signal directly proportional to ionic strength in sample cell, conductance signal is again through A/D
Converter is converted into digital signal and is transferred to embedded acquisition controller, and embedded acquisition controller obtains liquid in sample cell
Obtained uv transmittance and conductivity are transferred to nucleic acid egg by interface controller by conductivity, embedded acquisition controller
White analysis work station is shown.
Preferably, the characteristic wavelength of the ultraviolet source is respectively the light source of 260nm and the light source of 280nm.
Further, the nucleic acid protein chromatographic work station receives data, screen draws spectrum, Parameter analysis, spectrogram editor, number
According to preservation, and map is shown on the screen.
Compared with prior art, the invention has the following advantages:
The present invention with ultra-violet light-emitting module (chip) for light source, by fiber coupling and beam path alignment system, by light source irradiate into
Enter sample cell, receives ultraviolet transmissive light with photoelectric sensor.After small-signal is amplified, the light intensity through sample cell is measured
Value handles using microprocessor (MCU) and obtains the uv transmittance of sample cell liquid.The present apparatus measurement 280nm and
While 260nm absorbs, the ionic strength of liquid also can measure, touch for college teaching experiment and biological medicine research and development technology personnel
Rope elution separation condition, formulation elution processes and later period, which further isolate and purify, provides a kind of highly efficient unique equipment.
The present apparatus is compact to design, system is stable, easy to operate, data acquire, software analysis integrates.
Detailed description of the invention
Fig. 1 is nucleic acid-protein testing principle block diagram；
Fig. 2 is implementation method figure of the invention；
Fig. 3 be in the present invention detection device to albumen wash-out experimental test figure；
Fig. 4 be in the present invention detection device to map editor (compression) figure of albumen wash-out experimental test figure.
Specific embodiment
Below with reference to embodiment, the present invention will be further explained.
As illustrated in fig. 1 and 2, a kind of nucleic acid-protein detection device based on LED light source, detection device include ultra-violet light-emitting mould
Block, photoelectricity collimation reception device, small-signal amplification module, conductivity sensor, excitation conversion module, Conductivity detection amplify mould
Block, logarithmic converter, phase-sensitive detector, A/D converter, embedded acquisition controller, interface controller and chromatography work station.Institute
Stating photoelectricity collimation reception device includes fiber coupler, sample cell and ultraviolet light transducer, in which: embedded acquisition controller electricity
It connects and controls ultra-violet light-emitting module and issue ultraviolet source, the ultraviolet source that ultra-violet light-emitting module issues is irradiated through fiber coupler
Into sample cell, ultraviolet light passes through and receives ultraviolet transmissive light by ultraviolet light photo sensor after sample cell, ultraviolet light photo sensor according to
Secondary electrical connection small-signal amplification module and logarithmic converter；Conductivity sensor is installed, conductivity sensor is successively electric in sample cell
Connection excitation conversion module, amplifier module and phase-sensitive detector, the logarithmic converter and phase-sensitive detector are turned by A/D
Parallel operation is electrically connected embedded acquisition controller, and embedded acquisition controller is electrically connected nucleic acid protein chromatographic work by interface controller
It stands；The signal of the ultraviolet light photo sensor successively obtains sample cell liquid through small-signal amplification module and logarithmic converter
To the absorption signal of ultraviolet light；The signal of conductivity sensor is successively through excitation conversion module, amplifier module and phase-sensitive detector
Obtain the conductance signal (ionic strength) of sample cell liquid；The absorption signal and conductance signal are transferred to embedding through A/D converter
Enter formula acquisition controller, embedded acquisition controller obtains uv transmittance and conductivity, and embedded acquisition controller passes through
Uv transmittance and conductivity are transferred to nucleic acid protein chromatographic work station by interface controller；The nucleic acid protein chromatographic work
It stands and carries out data receiver, screen draws spectrum, Parameter analysis, spectrogram editor, data preservation work.
Preferably, the characteristic wavelength for the ultraviolet source that the ultra-violet light-emitting module issues be respectively 260nm light source and
The light source of 280nm.
In particular, the ultraviolet source that ultra-violet light-emitting module issues is sent to sample cell through optical fiber by the fiber coupler.
Preferably, the capacity of the sample cell is 70ul, light path 4mm.
Preferably, the model GUVB-T11GD of the ultraviolet light transducer.
Preferably, the MCU in the embedded acquisition controller is ARM, model STM32F103 series.
In particular, the uv transmittance of liquid and conductivity data work in nucleic acid protein chromatographic in the sample cell
Real-time display on the screen stood.
As illustrated in fig. 1 and 2, a kind of detection method of the nucleic acid-protein detection device based on LED light source, after chromatographic column processing
Sample be placed in sample cell, the sample flowed out in sample cell is collected by part collector；The part collector is independent quotient
Product use after can directly buying；Embedded acquisition controller control ultra-violet light-emitting module issues a branch of or two beam ultraviolet sources,
Ultraviolet source is irradiated into sample cell through fiber coupler, and ultraviolet source is through sample cell and transmission enhancement is in ultraviolet light photo sensor,
Ultraviolet light photo sensor obtains successively amplifying mould through small-signal with the directly proportional absorption signal of transmitted intensity, absorption signal
Block and logarithmic converter, the absorption signal intensity and sample cell absorbance obtained at this time is in direct ratio, and absorption signal is again through A/D
Converter is converted into digital signal and is transferred to embedded acquisition controller, and embedded acquisition controller obtains liquid in sample cell
Uv transmittance；Meanwhile the conductance signal that the conductivity sensor being arranged in sample cell will obtain, conductance signal is successively through motivating
Conversion module, Conductivity detection amplification module and phase-sensitive detector, obtain the conductance signal directly proportional to ionic strength in sample cell,
Conductance signal is converted into digital signal through A/D converter again and is transferred to embedded acquisition controller, and embedded acquisition controller obtains
Obtained uv transmittance and conductivity are passed through interface control by the conductivity of liquid in sample cell, embedded acquisition controller
Device processed is transferred to nucleic acid protein chromatographic work station and shows.
Preferably, the characteristic wavelength of the ultraviolet source is respectively the light source of 260nm and the light source of 280nm.
In particular, the nucleic acid protein chromatographic work station receives data, screen draws spectrum, Parameter analysis, spectrogram editor, number
According to preservation, and map is shown on the screen.
Photomultiplier tube is substituted using ultraviolet light photo receiver (ultraviolet light photo sensor) in the present invention.The ultraviolet light passes
Sensor model can be GUVB-T11GD etc..
As shown in Figures 3 and 4, it is detected using detection device provided by the invention to albumen wash-out experimental test figure；To albumen
Map editor (compression) figure of elution test test chart.
Detection device of the invention has the following characteristics that
1, the synchronous detection of dual wavelength (260nm 280nm), conductivity, technological innovation.
2, without preheating ultraviolet LED light source, technological innovation.
3, for device adjust automatically absorbance to 0.000, intelligence is convenient.
4, the absorbance and ionic strength (conductivity) of device real-time display dual wavelength.
5, chromatography work station has the function of spectral data acquisition, analytical calculation, map editor, preservation, printing etc..
6, device is furnished with USB interface, the system integration.
7, a computer can match more detection devices (being determined by the effective USB port number of computer).
Nucleic acid protein chromatographic work station is the computer (desktop computer or notebook) for being equipped with chromatography software for analyzing spectrum, tool
Body function is as follows:
1, by measurement selection menu, absorbance (A) spectrogram can be described on computer screen.
2, by menus such as figure translation, time stretching, extension and amplitude scaling selections, can go forward side by side to spectrogram line amplitude, width tune
Whole and spectral parameters calculate, and print out after preview is satisfied.
3, during retouching spectrum, figure can be moved to left and (can also be manually adjusted) automatically by computer, and computer acquisition is write from memory between retouching time spectrum
Recognize 10 hours.(longest 20 hours)
4, data are acquired, analysis map, editor's map, are automatically saved.
Nucleic acid-protein detection device major parameter based on LED light source:
1, Detection wavelength: 260nm, 280nm.
2, sample cell 70ul, light path 4mm.
3, range: absorbance (A): 0--2.000；Conductivity (K): 10-5ms--10-1ms。
4, resolution ratio: absorbance (A): 0.001.
5, work station analyzes parameter: peak height, peak width, peak area, area normalization, retention time, volume content, purity point
Analysis, chromatography column resolution etc..
6, power supply 220V ± 10%, 50HZ.
7, host weight: about 2.5Kg.
The above is only a preferred embodiment of the present invention, it should be pointed out that: those skilled in the art are come
It says, under the premise of not departing from feature of present invention, several improvements and modifications can also be made, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (9)
1. a kind of nucleic acid-protein detection device based on LED light source, it is characterised in that: detection device include ultra-violet light-emitting module,
Photoelectricity collimates reception device, small-signal amplification module, conductivity sensor, excitation conversion module, Conductivity detection amplification module, right
Number converter, phase-sensitive detector, A/D converter, embedded acquisition controller, interface controller and chromatography work station, the light
Electricity collimation reception device includes fiber coupler, sample cell and ultraviolet light transducer, in which:
Embedded acquisition controller, which is electrically connected and controls ultra-violet light-emitting module, issues ultraviolet source, the purple that ultra-violet light-emitting module issues
Outer light source is irradiated into sample cell through fiber coupler, and it is purple that ultraviolet light receives transmission by ultraviolet light photo sensor after sample cell
Outer light, ultraviolet light photo sensor are sequentially connected electrically small-signal amplification module and logarithmic converter；Installation conductance passes in sample cell
Sensor, conductivity sensor are sequentially connected electrically excitation conversion module, amplifier module and phase-sensitive detector, the logarithmic converter and
Phase-sensitive detector passes through A/D converter and is electrically connected embedded acquisition controller, and embedded acquisition controller passes through Interface Controller
Device is electrically connected nucleic acid protein chromatographic work station；
The signal of the ultraviolet light photo sensor successively obtains sample cell liquid through small-signal amplification module and logarithmic converter
To the absorption signal of ultraviolet light；The signal of conductivity sensor is successively through excitation conversion module, amplifier module and phase-sensitive detector
Obtain the conductance signal of sample cell liquid；The absorption signal and conductance signal are transferred to embedded acquisition through A/D converter and control
Device processed, embedded acquisition controller obtain uv transmittance and conductivity, and embedded acquisition controller passes through interface controller
Uv transmittance and conductivity are transferred to nucleic acid protein chromatographic work station；
The nucleic acid protein chromatographic work station carries out data receiver, screen draws spectrum, Parameter analysis, spectrogram editor, data preservation work
Make.
2. the nucleic acid-protein detection device according to claim 1 based on LED light source, it is characterised in that: the ultraviolet hair
The characteristic wavelength for the ultraviolet source that optical module issues is respectively the light source of 260nm and the light source of 280nm.
3. the nucleic acid-protein detection device according to claim 1 based on LED light source, it is characterised in that: the optical fiber coupling
The ultraviolet source that ultra-violet light-emitting module issues is sent to sample cell through optical fiber by clutch.
4. the nucleic acid-protein detection device according to claim 1 based on LED light source, it is characterised in that: the ultraviolet light
Sensor model number is GUVB-T11GD.
5. the nucleic acid-protein detection device according to claim 1 based on LED light source, it is characterised in that: described embedded
MCU in acquisition controller is ARM, model STM32F103 series.
6. the nucleic acid-protein detection device according to claim 1 based on LED light source, it is characterised in that: the sample cell
The uv transmittance and conductivity data of interior liquid real-time display on the screen of nucleic acid protein chromatographic work station.
7. the detection method of -6 any nucleic acid-protein detection devices based on LED light source according to claim 1, feature
Be: treated that sample is placed in sample cell for chromatographic column, and embedded acquisition controller control ultra-violet light-emitting module issues a branch of
Or two beam ultraviolet sources, ultraviolet source are irradiated into sample cell through fiber coupler, ultraviolet source through sample cell and transmission enhancement in
Ultraviolet light photo sensor, ultraviolet light photo sensor obtain with the directly proportional absorption signal of transmitted intensity, absorption signal is successively
Through small-signal amplification module and logarithmic converter, the absorption signal intensity obtained at this time is directly proportional to sample cell absorbance
Example, absorption signal are converted into digital signal through A/D converter again and are transferred to embedded acquisition controller, embedded acquisition controller
Obtain the uv transmittance of liquid in sample cell；Meanwhile the conductance signal that the conductivity sensor being arranged in sample cell will obtain,
Conductance signal successively through excitation conversion module, Conductivity detection amplification module and phase-sensitive detector, obtains strong with ion in sample cell
Directly proportional conductance signal is spent, conductance signal is converted into digital signal through A/D converter again and is transferred to embedded acquisition controller,
Embedded acquisition controller obtains the conductivity of liquid in sample cell, the uv transmittance that embedded acquisition controller will obtain
Nucleic acid protein chromatographic work station is transferred to by interface controller with conductivity to show.
8. the detection method of the nucleic acid-protein detection device according to claim 7 based on LED light source, it is characterised in that:
The characteristic wavelength of the ultraviolet source is respectively the light source of 260nm and the light source of 280nm.
9. the detection method of the nucleic acid-protein detection device according to claim 7 based on LED light source, it is characterised in that:
Nucleic acid protein chromatographic work station receives data, screen draws spectrum, Parameter analysis, spectrogram editor, data preservation, and shows on the screen
Map.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910444790.5A CN110133144A (en) | 2019-07-03 | 2019-07-03 | Nucleic acid-protein detection device and method based on LED light source |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910444790.5A CN110133144A (en) | 2019-07-03 | 2019-07-03 | Nucleic acid-protein detection device and method based on LED light source |
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| CN110133144A true CN110133144A (en) | 2019-08-16 |
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Application publication date: 20190816 |