CN205484221U - Aflatoxin liquid chromatogram detector - Google Patents
Aflatoxin liquid chromatogram detector Download PDFInfo
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- CN205484221U CN205484221U CN201521111820.4U CN201521111820U CN205484221U CN 205484221 U CN205484221 U CN 205484221U CN 201521111820 U CN201521111820 U CN 201521111820U CN 205484221 U CN205484221 U CN 205484221U
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Abstract
The utility model discloses an aflatoxin liquid chromatogram detector, its characterized in that: the detector including deriving light source (1), be equipped with tetrafluoro pipe (2) with the light source of deriving (1) matches, the entry of tetrafluoro pipe (2) links to each other with the chromatographic column, the export and fluorescence detection pond (3) the intercommunication of tetrafluoro pipe (2) are provided with dichroic mirror (4) with fluorescence detection pond (3) matches, are provided with excitation light source (5) and glimmering light detector (6) with dichroic mirror (4) matches, just excitation light source (5) be located the reflected light path direction of dichroic mirror (4), glimmering light detector (6) are located the transmitted light path direction of dichroic mirror (4), glimmering light detector (6) link to each other through circuit system (7) and chromatographic work station. This is a convenient operation, low price, aflatoxin liquid chromatogram detector that maintenance cost is low.
Description
Technical field
This utility model relates to a kind of detector, particularly a kind of aflatoxin liquid chromatogram detector.
Background technology
Aflatoxin is a kind of length Aspergillus flavus in food and feedstuff and the one of aspergillus parasiticus metabolism group of product that chemical constitution is similar.It is primarily present in Semen arachidis hypogaeae, corn, kernel, rice, Chinese medicine and the food such as Related product and milk product thereof gone mouldy, and is the extremely strong material of a kind of toxicity.The hazardness of aflatoxin is that people and animal livers tissue are had destruction, may result in hepatocarcinoma the most dead time serious.Therefore, the detection for aflatoxin is the most necessary.
The detection method of current aflatoxin mainly has thin layer chromatography, enzyme linked immunosorbent assay and high performance liquid chromatography etc..The detector that wherein high-efficient liquid phase color method spectrum uses mainly includes following two: 1, fluorescence detector, the characteristic utilizing aflatoxin to have fluorescence detects, excitation wavelength 360nm, launches wavelength 420nm；2, mass detector, highly sensitive, qualitative accurately such as use triple level Four bar, positive ion mode MRM monitoring ion pair carries out qualitative and quantitative analysis.
Derivative is to utilize derivative reaction to make determinand generate derivant with corresponding derivatization reagent, to change the physicochemical property of determinand.Therefore, when configuration fluorescence detector detects, in order to improve aflatoxin B1、G1Fluorescence intensity, main use following two method to improve fluorescence intensity, and then improve detection sensitivity: 1, column front derivation, as used trifluoroacetic acid (TFA) as the chemical derivatization of derivative reagent；2, post-column derivation, including the chemical post-column derivation method with bromine or iodine as derivative reagent and photochemistry post-column derivation method.In addition to photochemistry post-column derivation method, being required for using other chemical reagent and needing the special derivatization device of supporting correspondence, not only solution preparation is loaded down with trivial details, experimental implementation bothers, and pipeline connects complexity, and derivative reagent exists poisonous and hazardous risk, it is unfavorable for the commercialization of detector, universalness.
In sum, being currently used in the liquid chromatogram detector of aflatoxin, although mass detector testing result is good, but instrument price is the highest, and needs high use and maintenance cost, is unfavorable for the universal of detection method.The fluorescence detector price that fluorescence detection uses is relatively low relative to mass spectrum, but instrument is limited by the xenon lamp life-span and often needs higher maintenance cost.
Summary of the invention
This utility model is to solve the above-mentioned deficiency existing for prior art, proposes a kind of easy to operate, low price, the aflatoxin liquid chromatogram detector that maintenance cost is low.
nullTechnical solution of the present utility model is: a kind of aflatoxin liquid chromatogram detector，It is characterized in that: described detector includes derivative light source 1，It is provided with four fluorine tube 2 with derivative light source 1 matches，The entrance of four fluorine tube 2 is connected with column outlet，The outlet of four fluorine tube 2 connects with fluorescence detection cell 3，It is provided with dichroscope 4 with fluorescence detection cell 3 matches，It is provided with excitation source 5 and fluorescent probe 6 with dichroscope 4 matches，And described excitation source 5 is positioned on the reflected light path direction of dichroscope 4，Fluorescent probe 6 is positioned on the transmitted light path direction of dichroscope 4，Described fluorescent probe 6 is connected with chromatographic work station by Circuits System 7，Described derivative light source 1 is the uviol lamp of 254nm，Described excitation source 5 is the light emitting diode of 365nm，The port of export of described fluorescence detection cell 3 is connected with waste liquid barrel.
This utility model compared with the existing technology, has the advantage that
The aflatoxin liquid chromatogram detector of this kind of version, photochemistry post-column derivation device originally and the function of two instruments of fluorescence detector can be realized with an instrument, not only reduce cost and the price of instrument, by integrated, instrument mechanical dimension is substantially reduced, and has the feature of miniaturization；And its intrasystem dead volume is substantially reduced, and improves aflatoxin detection sensitivity simultaneously.And light emitting diode has the feature of life-span length, low cost as excitation source compared to xenon lamp, this makes the use of instrument and maintenance cost greatly reduce；Further, since photochemistry post-column derivation need not use any reagent, the most also decrease the pollution to environment that derivative reagent brings and the murder by poisoning problem to people.The above-mentioned number of advantages possessed just because of this aflatoxin specific detectors, is particularly suitable for liquid-phase chromatographic analysis aflatoxin in analysis field, and market prospect is the most wide.
Accompanying drawing explanation
Fig. 1 is the structural representation of this utility model embodiment.
Fig. 2 is the actually detected chromatograms of aflatoxin standard substance.
Fig. 3 is aflatoxin B1With G1Light derives schematic diagram.
Detailed description of the invention
Below in conjunction with accompanying drawing, detailed description of the invention of the present utility model is described.Such as Fig. 1, Fig. 2, shown in Fig. 3: a kind of aflatoxin liquid chromatogram detector, it includes derivative light source 1, this derivative light source is the uviol lamp of 254nm, derivative light source 1 match be provided with four fluorine tube 2, the arrival end of this four fluorine tube 2 is connected with column outlet, the port of export of four fluorine tube 2 is then connected with fluorescence detection cell 3, it is provided with dichroscope 4 with fluorescence detection cell 3 matches, it is provided with excitation source 5 and fluorescent probe 6 with this dichroscope 4 matches, and described excitation source 5 is positioned on the reflected light path direction of dichroscope 4, fluorescent probe 6 is then positioned on the transmitted light path direction of dichroscope 4, excitation source 5 is the light emitting diode of 365nm simultaneously；Above-mentioned fluorescent probe 6 is connected with chromatographic work station by Circuits System 7, and the outlet of fluorescence detection cell 3 is then connected with waste liquid barrel.
The work process of the aflatoxin liquid chromatogram detector of this utility model embodiment is as follows: aflatoxin sample is after chromatographic column (high performance liquid chromatography) separates, enter four fluorine tube 2, and at derivative light source 1(254nm ultraviolet light) irradiation under derived, to improve B1With G1Fluorescence intensity, sample after photochemical derivatization is directly entered fluorescence detection cell 3 afterwards, the light that excitation source (Light-Emitting Diode of 365nm) produces is after the reflection of dichroscope 4, sample in fluorescence detection cell 3 is excited, produce the fluorescence of 430nm wavelength, this part fluorescence is through entering fluorescent probe 6 after dichroscope 4, fluorescence signal is changed into the signal of telecommunication by fluorescent probe 6, after eventually passing Circuits System 7 process, chromatographic signal is passed to chromatographic work station to be analyzed, detection, sample after being excited by fluorescence detection cell 3 is then directly discharged into waste liquid barrel.
As in figure 2 it is shown, Fig. 2 is the Sensitivity comparison result after this device photochemical derivatization operates.
Use EClassical 3100 high performance liquid chromatograph to configure this aflatoxin specific detectors and analyze aflatoxin B1、G1(20 μ g/L) and B2、G2(6 μ g/L), concrete chromatographic condition is as follows: chromatographic column is SinoChrom ODS-BP 5 μm, ID4.6 × 200mm, and flowing is methanol/acetonitrile/water (20/20/60, v/v/v) solution mutually, and flow is 0.8mL/min, and sample size is 20 μ L, room temperature.
As it is shown on figure 3, Fig. 3 is aflatoxin B1With G1Photochemical derivatization schematic diagram, wherein (a) is aflatoxin B1Becoming B2a through photochemical derivatization hydroxylating, (b) is AFG1G2a is become through photochemical derivatization hydroxylating.
Claims (1)
- null1. an aflatoxin liquid chromatogram detector，It is characterized in that: described detector includes derivative light source (1)，It is provided with four fluorine tube (2) with derivative light source (1) matches，The entrance of four fluorine tube (2) is connected with column outlet，The outlet of four fluorine tube (2) connects with fluorescence detection cell (3)，It is provided with dichroscope (4) with fluorescence detection cell (3) matches，It is provided with excitation source (5) and fluorescent probe (6) with dichroscope (4) matches，And described excitation source (5) is positioned on the reflected light path direction of dichroscope (4)，Fluorescent probe (6) is positioned on the transmitted light path direction of dichroscope (4)，Described fluorescent probe (6) is connected with chromatographic work station by Circuits System (7)，Described derivative light source (1) is the uviol lamp of 254nm，Described excitation source (5) is the light emitting diode of 365nm，The port of export of described fluorescence detection cell (3) is connected with waste liquid barrel.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521111820.4U CN205484221U (en) | 2015-12-29 | 2015-12-29 | Aflatoxin liquid chromatogram detector |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201521111820.4U CN205484221U (en) | 2015-12-29 | 2015-12-29 | Aflatoxin liquid chromatogram detector |
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| CN205484221U true CN205484221U (en) | 2016-08-17 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107037162A (en) * | 2016-12-30 | 2017-08-11 | 大连技嘉科技有限公司 | Photochemistry post-column derivation device |
| CN108088822A (en) * | 2016-11-21 | 2018-05-29 | 中国科学院大连化学物理研究所 | A kind of chip type light derivatization device of aflatoxin and sulfa drugs |
| CN110095549A (en) * | 2019-06-12 | 2019-08-06 | 吉林省产品质量监督检验院(吉林省农产品认证中心) | The detection device and method of aflatoxin in a kind of Grain and its product |
-
2015
- 2015-12-29 CN CN201521111820.4U patent/CN205484221U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108088822A (en) * | 2016-11-21 | 2018-05-29 | 中国科学院大连化学物理研究所 | A kind of chip type light derivatization device of aflatoxin and sulfa drugs |
| CN108088822B (en) * | 2016-11-21 | 2020-05-26 | 中国科学院大连化学物理研究所 | Chip type light derivatization device for aflatoxin and sulfanilamide drugs |
| CN107037162A (en) * | 2016-12-30 | 2017-08-11 | 大连技嘉科技有限公司 | Photochemistry post-column derivation device |
| CN110095549A (en) * | 2019-06-12 | 2019-08-06 | 吉林省产品质量监督检验院(吉林省农产品认证中心) | The detection device and method of aflatoxin in a kind of Grain and its product |
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