CN107764913A - A kind of nucleic acid-protein conductance combination detecting system and its detection method - Google Patents
A kind of nucleic acid-protein conductance combination detecting system and its detection method Download PDFInfo
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Abstract
The present invention provides a kind of nucleic acid-protein conductance combination detecting system, including dual wavelength nucleic acid-protein detection means, and it is furnished with the liquid chromatographic separation system that chromatographic column (3), constant flow pump (2), the embedded acquisition controller of fraction collector (4) and computer work (6) form complete set；In the dual wavelength nucleic acid-protein detection means dual wavelength nucleic acid-protein conductance combination detection means is formed equipped with conductive detection device, the combination detection means includes collimater, slit, sample cell and the photoelectric tube being linked in sequence, analog signal device, logarithmic converter and double channel A/D converters, it is furnished with conductance electrode in the sample cell, the conductance electrode is linked in sequence Conductivity detection amplifier, phase-sensitive detector and double channel A/D converters；The embedded acquisition controller connects computer work (6) by interface controller, and controls dual wavelength switching and the coordination of double channel A/D converters to change；The computer work (6) carry out data transmission receive, screen paint spectrum, Parameter analysis, spectrogram editor, data preservation work.
Description
Technical field
The present invention relates to biochemistry and field of biological pharmacy, and in particular to a kind of nucleic acid-protein-conductance combination detection system
System and its detection method.
Background technology
During bioscience and bio-pharmaceuticals are researched and produced, nucleic acid-protein detection means is non-in chromatographic assay system
Often important detection device.Mix chromatographic column, constant flow pump, fraction collector (apolegamy as needed) and computerized print equipment i.e. structure
Into the liquid chromatographic separation system of complete set.Turn into and be engaged in life science, drug monitoring, chemical industry, environmental protection, food now
The modern analysis laboratory apparatus of the industry such as product science and medical research.It is widely used in the section of industry, agricultural, scientific research and universities and colleges
Learn research and teaching experiment.Its principle is with the feature substantially absorbed, to sample according to material (sample) to ultraviolet certain wave
Component content compares analysis, to carry out the Object Classifications such as sample protein, nucleic acid and assay.Although the egg of current domestic production
White detector species is various, but using Single wavelength detection (254nm, 280nm ...).These detectors can only select certain in measurement
One wavelength (280nm or 254nm) detection tie substance (albumen or nucleic acid), and in system coexists in protein nucleic acid etc., a sublevel
Analysis can not measure the purity of albumen (or nucleic acid), and twice chromatographic needs double experimental period to can guarantee that identical tests bar again
Part.Double UV check not only has the repertoire of Single wavelength measurement, and once can be with the suction of detectable substance confrontation different wave length
Receive, improve the science and conventional efficient of tomographic system.
The content of the invention
The purpose of the present invention is：A kind of nucleic acid-protein-conductance combination detecting system and its detection method are provided, are to complete
On the basis of " dual wavelength nucleic acid-protein detection means ", by the another item detection means completed after experimental demonstration repeatedly.Feature
It is the same Time absorbed in Measuring amounts 280nm and 260nm, the ionic strength of measurement eluent (mobile phase) (is eluted with electrical conductivity is corresponding
The ionic strength of liquid), grope nucleic acid-protein lightning strip for universities and colleges' teaching and scientific research experiment and biological medicine research and development technology personnel
Part, formulation elution processes and later stage further isolate and purify and provide a kind of highly efficient unique equipment.Single unit system designs
It is compact, system is stable, easy to operate, data acquisition, software analysis integrate.
The technical scheme is that：A kind of nucleic acid-protein-conductance combination detecting system, including the inspection of dual wavelength nucleic acid-protein
Device is surveyed, and is structure equipped with chromatographic column, constant flow pump, fraction collector and the computer work with embedded acquisition controller
Into the liquid chromatographic separation system of complete set；
In the dual wavelength nucleic acid-protein detection means dual wavelength nucleic acid-protein-conductance connection is formed equipped with conductive detection device
With detection means, the conductive detection device includes collimater, slit, sample cell and the photoelectric tube being linked in sequence, simulation letter
Number device, logarithmic converter and double channel A/D converters, it is furnished with conductance electrode in the sample cell, the conductance electrode order connects
Connect Conductivity detection amplifier, phase-sensitive detector and double channel A/D converters；
The embedded acquisition controller connects computer work by interface controller, and controls dual wavelength switching and double
The coordination conversion of passage A/D converter；
The computer work carry out data transmission receive, screen paint spectrum, Parameter analysis, spectrogram editor, data preservation work
Make.
Further, the conductance electrode is two electrodes, and uses high frequency dipulse or square wave as alternating excitation source.Use
Phase sensitive detection technology, effectively improves the stability of a system.
Further, the interface controller is furnished with com port and USB interface.
Further, the double wave a length of 260nm and 280nm.
The present invention also provides nucleic acid-protein-electricity that nucleic acid-protein-conductance combination detecting system described in a kind of basis is realized
Lead detection method, is comprised the following steps that：
Step 1: gather two beam ultraviolet source monochrome light absorbs data using liquid chromatographic separation system；
Step 2: two beam ultraviolet source monochrome light projections are entered onto collimater, it is saturating respectively after slit is mapped to sample cell
It is mapped on photoelectric tube, two-way and the directly proportional electric signal of transmitted intensity is obtained through opto-electronic conversion；
Step 3: under the control of embedded acquisition controller, first two-way analog signal is amplified, then carries out signal logarithm
Conversion, the signal now obtained and sample cell light absorbs are in direct ratio, then the signal now obtained is changed into shape through double channel A/D
Into data signal；
Step 4: being furnished with conductance electrode in sample cell, excitation change-over circuit is obtained into the alternating signal related to electric conductivity value,
The electric signal directly proportional to ionic strength in sample cell is obtained through signal amplification and phase sensitive detection；
Step 5: the ionic strength in sample cell is can obtain after being calibrated with KCL solution.
Further, the two beams ultraviolet source wavelength is 260nm and 280nm.
Further, two beam ultraviolet source monochrome light absorbs data of collection and sample cell conductivity data are directly in computer
The screen display collection of illustrative plates of work station.
The beneficial effects of the invention are as follows：
1) " nucleic acid-protein conductance is combined detection means " is on the basis of " dual wavelength nucleic acid-protein detection means " is completed, to pass through
The multi-functional chromatography detection means of another item completed repeatedly after experimental demonstration.Feature Shi Measuring amounts 280nm and 260nm absorb
Same Time, Measuring amount wash the ionic strength (ionic strength that Off liquid is correspondingly washed with electrical conductivity) of Off liquid (mobile phase).Taught for universities and colleges
It is further from Strip parts, formulation elution processes and later stage that scientific experiment and biological medicine research and development technology personnel grope nucleic acid-protein point
Isolate and purify and provide a kind of highly efficient unique equipment.Engagement positions are compact to design, system is stable, easy to operate, data are adopted
Collection, software analysis integrate.
2) " nucleic acid-protein conductance combination detection work station " is the core component of " nucleic acid-protein conductance is combined detection means ".
Gather 260nm and 280nm and absorb data and sample cell conductivity data directly in screen display collection of illustrative plates, peak is carried out to absworption peak
The parameter such as height, standard deviation, half-peak breadth, peak base is wide, peak area, Return mono- change, area fraction and retention time is calculated.
3) the use of electric conductivity detector is two electrodes in the present apparatus, uses high frequency dipulse or square wave to make for alternating excitation source
With phase sensitive detection technology, the stability of a system is effectively improved.
The present invention is on the basis of " dual wavelength nucleic acid-protein detection means " is completed, by what is completed after experimental demonstration repeatedly
Another item detection means.Measuring amounts 280nm and 260nm absorb Tong Time, the ionic strength (electricity consumption of measurement eluent (mobile phase)
Conductance corresponds to the ionic strength of eluent), grope core for universities and colleges' teaching and scientific research experiment and biological medicine research and development technology personnel
Acid albumin separation condition, formulation elution processes and later stage further isolate and purify and provide a kind of highly efficient unique equipment.
Single unit system is compact to design, system is stable, easy to operate, data acquisition, software analysis integrate.
Brief description of the drawings
Fig. 1 is that nucleic acid-protein-conductance is combined detecting system overall structure diagram；
Fig. 2 is that nucleic acid-protein-conductance is combined detecting system partial schematic diagram；
Fig. 3 is that nucleic acid-protein-conductance is combined detecting system elution test test chart.
In figure：1 is gradient eluent, and 2 be constant flow pump, and 3 be chromatographic column, and 4 be fraction collector, and 5 be nucleic acid-protein-conductance
Detection means is combined, 6 be computer work, and 7 be mobile phase (ionic strength change) gradient elution spectrogram, and 8 be that absorbing proteins are composed
Figure, 9 be that nucleic acid absorbs spectrogram.
Embodiment
The present invention is described further below in conjunction with the accompanying drawings.
As shown in Figure 1, 2, a kind of nucleic acid-protein-conductance combination detecting system, including dual wavelength nucleic acid-protein detection means,
And it is furnished with chromatographic column 3, constant flow pump 2, fraction collector 4 and the computer work 6 with embedded acquisition controller and forms
The liquid chromatographic separation system of complete set；
In the dual wavelength nucleic acid-protein detection means dual wavelength nucleic acid-protein-conductance connection is formed equipped with conductive detection device
With detection means, the conductive detection device includes collimater, slit, sample cell and the photoelectric tube being linked in sequence, simulation letter
Number device, logarithmic converter and double channel A/D converters, it is furnished with conductance electrode in the sample cell, the conductance electrode order connects
Connect Conductivity detection amplifier, phase-sensitive detector and double channel A/D converters；
The embedded acquisition controller by interface controller connect computer work 6, and control dual wavelength switching and
The coordination conversion of double channel A/D converters；
The computer work 6 carry out data transmission receive, screen paint spectrum, Parameter analysis, spectrogram editor, data preservation work
Make.
The conductance electrode is two electrodes, and uses high frequency dipulse or square wave as alternating excitation source.Use phase sensitive detection
Technology, effectively improve the stability of a system.
Nucleic acid-protein-conductance combination detection method, is comprised the following steps that：
Step 1: gather two beam ultraviolet source monochrome light absorbs data using liquid chromatographic separation system；
Step 2: two beam ultraviolet source monochrome light projections are entered onto collimater, it is saturating respectively after slit is mapped to sample cell
It is mapped on photoelectric tube, two-way and the directly proportional electric signal of transmitted intensity is obtained through opto-electronic conversion；
Step 3: under the control of embedded acquisition controller, first two-way analog signal is amplified, then carries out signal logarithm
Conversion, the signal now obtained and sample cell light absorbs are in direct ratio, then the signal now obtained is changed into shape through double channel A/D
Into data signal；
Step 4: being furnished with conductance electrode in sample cell, excitation change-over circuit is obtained into the alternating signal related to electric conductivity value,
The electric signal directly proportional to ionic strength in sample cell is obtained through signal amplification and phase sensitive detection；
Step 5: the ionic strength in sample cell is can obtain after being calibrated with KCL solution.
The working environment of nucleic acid-protein-conductance combination detecting system is as follows：
First, major parameter：
1st, wavelength：260nm, 280nm.
2nd, sample cell 70ul, light path 3mm.
3rd, range：Absorbance (A)：0--2.000；Electricity Guide rates (K)：10-5--10-2
4th, resolution：Absorbance (A)：0.001
5th, computer for analysis parameter：Peak height, peak width, peak area, area normalization, retention time, volume content, purity point
Analysis, chromatographic column resolution etc..
6th, power supply 220V ± 10%, 50HZ.
7th, main frame weight：About 3.5Kg.
2nd, system installation and operating procedure
1st, it is the output end on instrument backboard is serial by COM1, COM2 of a serial port connecting cable and host computer
Mouth or USB port are connected.
2nd, Ultraviolet Detector power supply (indicator lamp Chang Liang) is opened, instrument preheats more than 30 minutes.
3rd, after opening computer, accompanying software (ZHD_MK, Help, data) is copied on hard disk in same catalogue.By the emperor himself one
Auto zero button on lower instrument panel, after instrument shows 0.000, double-click ZHD_MK.exe and start application software, system
Into collection (analysis) state.
4th, " measurement starts " is clicked under " detection operation " menu, computer starts to gather.
Stop gathering, click on " measurement terminates " menu under " detection operation ", be then shut off detector.
3rd, software for analyzing spectrum is chromatographed to use
1st, hardware requirement and USB port driving：
A, simplified form of Chinese Character Windows xp, win7 operating systems；
B, display resolution is 1024*768, small font, the configuration of 256 colors；
C, graphic printer；
D, the necessary normal work of computer system, and ensure that interface (COM or USB) is effective；
USB port drive programe installation method：1st, instrument runs ZHDUSB.EXE in the case of not connected computer USB interface；
2nd, instrument is connected with computer USB port with USB lines, computer can show find new equipment and occur install driver to
Lead, the installation that can complete USB port driver is answered according to acquiescence, last computer can show that new equipment can use.After
Just connected during use with the mouth with instrument (with serial ports without driver).
2nd, first allow detector to work after system connection is errorless, then perform application software ZHD_MK；
3rd, " the opening spectrogram " under file operation menu is clicked on, file operation dialog box occurs, opens the number on random disk
According to file (.ran), figure is opened, and is familiar with menu operation.Menu is described below：
A, have under " file operation " menu and open spectrogram, preserve spectrogram, printing spectrogram, Print Preview etc.；
B, there is measurement to start under " detection operation " menu, measure and terminate that (after measurement terminates, system is under application catalog
Generation " filename .TXT " files, this formatted file can be opened in Excel softwares, and can be posted in Word document and use)；
C, have after " spectrogram translation " menu to the left it is slow mobile [<], to the left it is fast mobile [<<], to the right it is slow mobile [>] and to the right
Mobile soon [>>]；
D, " spectrogram is redrawn " menu：Spectrum is retouched from starting point；Cleaning interference；Release compression；
E, " spectrogram overall picture " menu：Whole spectrograms are observed on screen.As shown in Figure 3.
F, " parameter selection " menu:Parameter analysis calculating can be carried out to spectrogram.Method is as follows：Under absorbance state, point
Hit left mouse button and choose baseline and time range (click on choose at first point for the first time, click on choose second point for the second time), click on
The peak height of " selection parameter " drop-down menu, standard deviation, half-peak breadth, peak base are wide, peak area, normalization, area fraction and when retaining
Between etc. parameter calculated, can also calculate chromatographic column resolution ratio；Double left button mouse click, you can cancel this calculating.
G, there are albumen (280nm), nucleic acid (260nm), electrical conductivity (ms) and purity order under " spectrogram is shown " menu, use mouse
Punctuate, which is hit, to be chosen or abandons.Purity curve represents a certain moment A280 and A260 ratios, is represented in figure with K, i.e. K=A280/
A260.In nucleic acid-protein mixed system, K values are pure protein more than 1.7, and K values are pure nucleic acid less than 0.5.
H, area fraction concept (absorptiometry scope：0-2.000, flow speed stability)
1st, the nucleic acid-protein amount (material) of outflow in certain unit interval is set as m (unit is volume), and liquid is dense in this time
Spend for C=k*h (h tie substances (absorption) height, k is correction factor, and numerical value is between 0-1), then have:M=C* Δs V=k*h*
V* Δs T (Δ V is volume element here, and v is flow velocity, and Δ T is tempon).Therefore, in trickle in certain section seclected time
The nucleic acid-protein scale of construction (M) should be the cumulative of each tempon m, i.e.,:
M=Σ m=Σ k*h*v* Δ T=k*v* Σ h* Δs T=k*v*A is 1.
A is peak area.
2 but the total amount that is located at trickle in this time section be W, have：
W=Σ Δ V=Σ H*v* Δ T=v* Σ H* Δs T=v*S is 2.
H is liquid height, is here 2.000 (all absorbing) as should be a constant without disconnected liquid, H values in elution process.
Volume content (M/W) is extremely important parameter in biochemical analysis and extraction purification work.By 1. and 2., volume contains
(M/W)=k* (A/S) is measured, thus we provide the area fraction concept corresponding with volume content：
Area fraction=A/S
By measurement standard sample, correction factor k numerical value can be obtained.Later under same experimental conditions, pass through face
Product content value can obtain volume content numerical value.
I, after baseline is selected, (" ") , Ci Time double click right buttons are marked in summit position can for computer automatic Peak Search
Peak is selected in cancellation, and double click right button recovers to select peak again.Find out Bao Liu Time Inter that each peak is can print out behind peak, peak height, peak area,
Area normalization (preceding 15 peaks).
J, during unselected peak, click right can show the Bao Liu Time Inter of the point.
J, left button is double-clicked, cancels screen analyze data (cls).
4th, points for attention：
A, can not be by zero-setting button (particularly after appearance) after measurement starts.
B, to stop gathering, after " measurement terminates " please be click on, first click on " EXIT ", turn off detector.
When c, starting measurement, screen can eject preservation FileDialog, it is desirable to input data file name and storage path；It
Afterwards, computer automatically saves data.
H, baseline, which is chosen, will ensure that baseline must have two focuses with selected peak, and with other peaks without focus.
Note：1st, except especially naming, in text described " click ", single left button mouse click is referred both to.
2nd, instrumental working conditions should not have strong electromagnetic.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
- A kind of 1. nucleic acid-protein-conductance combination detecting system, it is characterised in that：Including dual wavelength nucleic acid-protein detection means, and Equipped with chromatographic column（3）, constant flow pump（2）, fraction collector（4）Embedded acquisition controller and computer work（6）Form one Cover complete liquid chromatographic separation system；In the dual wavelength nucleic acid-protein detection means dual wavelength nucleic acid-protein-conductance combination inspection is formed equipped with conductive detection device Survey device, it is described combination detection means include collimater, slit, sample cell and the photoelectric tube being linked in sequence, analog signal device, Logarithmic converter and double channel A/D converters, are furnished with conductance electrode in the sample cell, and the conductance electrode is linked in sequence conductance Detect amplifier, phase-sensitive detector and double channel A/D converters；The embedded acquisition controller connects computer work by interface controller（6）, and control dual wavelength switching and double The coordination conversion of passage A/D converter；The computer work（6）Carry out data transmission receive, screen paint spectrum, Parameter analysis, spectrogram editor, data preservation work Make.
- A kind of 2. nucleic acid-protein according to claim 1-conductance combination detecting system, it is characterised in that：The conductance electricity Extremely two electrodes, and high frequency dipulse or square wave are used as alternating excitation source.
- A kind of 3. nucleic acid-protein according to claim 1-conductance combination detecting system, it is characterised in that：The interface control Device processed is furnished with com port and USB interface.
- A kind of 4. nucleic acid-protein according to claim 1-conductance combination detecting system, it is characterised in that：The dual wavelength For 260nm and 280nm.
- A kind of 5. nucleic acid-protein-electricity realized according to any described nucleic acid-proteins of claim 1-4-conductance combination detecting system Lead detection method, it is characterised in that：Comprise the following steps that：Step 1: gather the monochromatic absorption data of two beam ultraviolet sources using liquid chromatographic separation system；Step 2: two beam ultraviolet source monochrome light projections are entered onto collimater, it is transmitted to respectively after slit is mapped to sample cell On photoelectric tube, two-way and the directly proportional electric signal of transmitted intensity are obtained through opto-electronic conversion；Step 3: under the control of embedded acquisition controller, first two-way analog signal is amplified, signal logarithm is then carried out and turns Change, the signal now obtained and sample cell light absorbs are in direct ratio, then the signal now obtained is changed to be formed through double channel A/D Data signal；Step 4: being furnished with conductance electrode in sample cell, excitation change-over circuit is obtained into the alternating signal related to electric conductivity value, through letter Number amplification and phase sensitive detection obtain the electric signal directly proportional to ionic strength in sample cell；Step 5: the ionic strength in sample cell is can obtain after being calibrated with KCL solution.
- 6. electric conductivity detecting method according to claim 5, it is characterised in that：The two beams ultraviolet source wavelength is 260nm And 280nm.
- 7. electric conductivity detecting method according to claim 5, it is characterised in that：Two beam ultraviolet source monochrome light absorbs of collection Data and sample cell conductivity data are directly in the screen display collection of illustrative plates of computer work.
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| CN110133144A (en) * | 2019-07-03 | 2019-08-16 | 南京大学 | Nucleic acid-protein detection device and method based on LED light source |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1973202A (en) * | 2004-05-20 | 2007-05-30 | 启龙有限公司 | Analysis of liquid chromatography eluates |
| CN201449370U (en) * | 2009-06-04 | 2010-05-05 | 南京大学 | Multi-wavelength nucleic acid protein chromatographic separation detection device |
| CN201837607U (en) * | 2010-10-15 | 2011-05-18 | 杭州泰林生物技术设备有限公司 | Measuring electrode for liquid electric conductivity |
| CN106018309A (en) * | 2016-05-05 | 2016-10-12 | 武汉光昱科技有限公司 | Multifunctional nucleic acid protein detection system |
| CN205844242U (en) * | 2016-07-25 | 2016-12-28 | 邓有鹏 | A kind of conductivity sensing electrodes |
| CN106929565A (en) * | 2015-12-30 | 2017-07-07 | 北京大学 | Protein monomolecular electronic device and its preparation and application based on nanostructured |
-
2017
- 2017-09-30 CN CN201710945093.9A patent/CN107764913A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1973202A (en) * | 2004-05-20 | 2007-05-30 | 启龙有限公司 | Analysis of liquid chromatography eluates |
| CN201449370U (en) * | 2009-06-04 | 2010-05-05 | 南京大学 | Multi-wavelength nucleic acid protein chromatographic separation detection device |
| CN201837607U (en) * | 2010-10-15 | 2011-05-18 | 杭州泰林生物技术设备有限公司 | Measuring electrode for liquid electric conductivity |
| CN106929565A (en) * | 2015-12-30 | 2017-07-07 | 北京大学 | Protein monomolecular electronic device and its preparation and application based on nanostructured |
| CN106018309A (en) * | 2016-05-05 | 2016-10-12 | 武汉光昱科技有限公司 | Multifunctional nucleic acid protein detection system |
| CN205844242U (en) * | 2016-07-25 | 2016-12-28 | 邓有鹏 | A kind of conductivity sensing electrodes |
Non-Patent Citations (1)
| Title |
|---|
| 罗德泉 等: "一种多通道相敏检波电路", 《电子技术应用》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110133144A (en) * | 2019-07-03 | 2019-08-16 | 南京大学 | Nucleic acid-protein detection device and method based on LED light source |
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