CN103499686A - Immunosensor-based resistance-type pesticide residue detector - Google Patents
Immunosensor-based resistance-type pesticide residue detector Download PDFInfo
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Abstract
The invention relates to an immunosensor-based resistance-type pesticide residue detector which is composed of a detection circuit, an immunosensor, a power source, buttons, a microcontroller and a display screen. The detection circuit is used for generating excitation voltage and applying the same on the immunosensor, and then sampling response signals after excitation, performing digital processing and sending a result to the microcontroller, the microcontroller and a detection program running on the same reprocess input signals and control on and respond to running of a whole system, the buttons are used for inputting instructions and detection parameters, the display screen is used for quantitatively outputting and presenting a final detection result and serves as an interaction interface of a user and a system, and the power source provides energy to the whole system. The immunosensor-based resistance-type pesticide residue detector integrates pesticide residue detection technology based on a biosensor method with an embedded-type system designing method, thereby having the advantages of high practicability, portability, high detection accuracy, quickness and accuracy in detection, easiness in operation and the like, and is quite suitable for occasions performing on-site quick detection on pesticide residues.
Description
Technical field
The present invention relates to a kind of instrument for detecting pesticide residue through, relate in particular to a kind of impedance type instrument for detecting pesticide residue through based on immunosensor, it has merged the Detection Technologies of Pesticide Residues and the Embedded System Design method of biology sensor method, for situation convenient, that detect rapidly, accurately Pesticide Residues.Belong to the Pesticides Testing technical field.
Background technology
Approximately 400,000 tons of the actual outputs in China's agricultural chemicals every year, be only second to the U.S. and occupy second place of the world, and approximately 270,000 tons of annual consumptions are at the forefront in the world, and the trend increased is year by year arranged.But because China's pesticide application technology falls behind, agricultural chemicals can not be utilized effectively, and the agricultural chemicals of excessively using has caused serious Pesticides Residues, also China's food security and human health has been formed to serious threat.
For China's agricultural production present situation that peasant household disperses production on a small scale, control residues of pesticides, with regard to must development carrying out the means that detect in real time fast to the residues of pesticides of fruit and vegetable surfaces, before the agricultural product listing, inspect by random samples on a large scale.The shortage of Fast Determination of Pesticide Residue method and backwardness are the technical bottlenecks that causes the export of farm produce to be moved back or destroyed.
General Detecting Pesticide method mainly can be divided into chromatographic detection and the large class of biochemistry detection method two.
The great advantage that chromatogram detects is the agricultural chemicals in can the detection sample of qualitative, quantitative, but the sample of this method will be through complicated pre-treatment step, detect personnel's operating equipment that length consuming time, cost are expensive, need number of techniques.
The biochemistry detection method is to detect the important method of residues of pesticides, and its principle is that the specific recognition of utilizing agricultural chemicals to carry out with the agricultural chemicals sensitive Protein reacts to judge the pollution by pesticides situation.During mensuration, sample preparation is simple, and instrument cost is low, simple to operate, and the screening that is applicable to Site Detection and gross sample detects, thereby is commonly called " method for quick ".The immunosensor detection method detects the advantages such as residues of pesticides have simple in structure, high specificity, cost is low, analysis speed is fast.
Immunosensor detects principle: utilize the antigen and antibody specific immune response, using antibody as recognition component, by immobilization technology, antibody is attached to susceptor surface, after with agricultural chemicals, immune recognition reaction occurring, the immune complex generated is associated with physics or the chemical signal of generation, and the resistance of itself is changed, and through mensuration and the standard value of oversampling circuit, does contrast, pass through again the conversion of program, finally obtain testing concentration information.
Summary of the invention
The purpose of patent of the present invention is for the deficiencies in the prior art, and design provides a kind of impedance type instrument for detecting pesticide residue through based on immunosensor, have easy to carry, detect quick and precisely, the characteristics such as simple to operate.
To achieve these goals, the impedance type instrument for detecting pesticide residue through based on immunosensor provided by the invention consists of testing circuit, immunosensor, power supply, button, microcontroller and display screen.Testing circuit is used for producing driving voltage and is applied on immunosensor, with the line number word processing of going forward side by side of the response signal after post-sampling excitation, result is sent into to microcontroller; Microcontroller and the trace routine of moving above thereof are processed again to input signal, and the operation of whole system is controlled and responded; Button is used for inputting instruction and detected parameters; Display screen is used for quantizing output and presents final testing result, is the interactive interface of user and system; Power supply provides energy for whole system.
Impedance type instrument for detecting pesticide residue through concrete structure based on immunosensor of the present invention is:
Detector consists of testing circuit, immunosensor, power supply, button, microcontroller and display screen, wherein:
Testing circuit is connected and composed by feed circuit, detection chip, toggle switch, demarcation resistance, immunosensor.Feed circuit are connected with detection chip with microcontroller, adopt first through transformer pressure-reducing, through the method for filtering voltage regulation, obtain again+the 5V power supply; Chip is connected with demarcation resistance by toggle switch, according to the input measurement range parameter, by toggle switch, selects corresponding demarcation resistance, with this, adjusts measurement sensitivity; Under the control of microcontroller, chip produces driving voltage and is applied to liquid to be measured and has occurred on the immunosensor of specific immune response and collected response signal, obtains its real part and imaginary part after DFT, and it is outputed to microcontroller.
Move trace routine on microcontroller, mould value and phase angle that mould value and phase calculation formula by the impedance real part that receives and imaginary part by impedance calculates impedance, draw actual resistance value again after calibration, with set react before resistance value compare, obtain rate of change and the contrast standard curve is reached a conclusion, deliver to display screen and show.
The display screen of telling is connected with microcontroller, and for showing data and the window that carries out interactive operation as the user, display screen adopts LED12864, and its power consumption can meet the power consumption requirement of portable set.
Described button is connected with testing circuit with microcontroller, is provided with startup, starts, resets, stops key and four sensing range gears, for inputting dependent instruction and detected parameters.
Described power supply is connected with feed circuit.
When detection of the present invention is used, at first select and demarcate resistance assurance measurement sensitivity by toggle switch, immunosensor is put into to solution to be detected, under the control of microcontroller, chip produce driving voltage be applied to liquid generation specific immune response to be measured after immunosensor on and collect response signal, obtain its real part and imaginary part after DFT, and it is outputed to microcontroller, carrying out signal through microcontroller and trace routine thereof processes again, and compare with reacting front resistance value, calculate rate of change.The size of impedance rate of change has shown to detect the concentration height of solution Pesticide Residues.
A kind of impedance type instrument for detecting pesticide residue through based on immunosensor provided by the invention is applied to the pollution by pesticides detection field, can bring great convenience for the testing staff, can fully meet the requirement of residues of pesticides Site Detection.Must be transported to inspection center to various testing samples with respect to testing in the past, by the professional staff, in large-scale station device, carried out, detector of the present invention have compact conformation, easy to carry, detect quick and precisely, the outstanding feature such as simple to operate, practical.
The accompanying drawing explanation
Fig. 1 is the structured flowchart that the present invention is based on the impedance type instrument for detecting pesticide residue through of immunosensor.
Fig. 2 is feed circuit schematic diagram in the present invention.
Fig. 3 is testing circuit schematic diagram in the present invention.
Embodiment
Below in conjunction with drawings and Examples, technical scheme of the present invention is elaborated.
At first, structural representation block diagram of the present invention is illustrated; The course of work of the feed circuit that secondly testing circuit comprised, detection chip, toggle switch, demarcation resistance is described in detail; Finally application software and the testing process of whole system are illustrated.
Fig. 1 is the structured flowchart that the present invention is based on the impedance type instrument for detecting pesticide residue through of immunosensor, and as shown in Figure 1, the present invention consists of testing circuit, immunosensor, power supply, button, microcontroller and display screen.Wherein testing circuit is connected and composed by feed circuit, detection chip, toggle switch, demarcation resistance, immunosensor.Testing circuit is used for producing driving voltage and is applied on immunosensor, with the line number word processing of going forward side by side of the response signal after post-sampling excitation, result is sent into to microcontroller; Microcontroller and the trace routine of moving above thereof are processed again to input signal, and the operation of whole system is controlled and responded; Button is used for inputting instruction and detected parameters; Display screen is used for quantizing output and presents final testing result, is the interactive interface of user and system; Power supply provides energy for whole system.
Described microcontroller is selected STC89C52, loads trace routine on it.
In one embodiment of the present of invention, feed circuit in testing circuit form and principle as shown in Figure 2, by transformer T1, bridge rectifier circuit BR1, electrochemical capacitor C1, C3, common capacitor C 2, C4, three terminal regulator U1 forms, and its effect is the Power supply for provide+5V of whole system.In the design, the effect of electrochemical capacitor C1, C2 is filtering, simply say while being exactly the rising of electric capacity both end voltage, and capacitor charging, during lower voltage, capacitor discharge, the gradient while allowing lower voltage becomes gently, thereby plays the effect of filtering.The effect of common capacitor C 2, C4 is to prevent self-excitation, and it can absorb the high frequency energizing signal to keep stable, makes circuit there is no pumping signal, thereby prevents self-excitation.The effect of three terminal regulator U1 be by output voltage stabilization on+5V, connect respectively+5V of the output and ground of U1 and ground connection.
In testing circuit, the formation of core testing circuit as shown in Figure 3: by detection chip U3, toggle switch SW3, demarcate resistance R 1, R2, R3, R4, R5, common capacitor C 7, C8 form, its effect is produce driving voltage and be applied on immunosensor, with the line number word processing of going forward side by side of the response signal after post-sampling excitation, result is sent into to microcontroller.The effect of wherein demarcating resistance R 1, R2, R3 is as external feedback resistance.Toggle switch SW3 is 4-bit DIP switch, and its effect is to select the resistance of suitable demarcation to be connected into testing circuit to prevent that response signal from surpassing the range of detection chip and guaranteeing the system linearity characteristic.The effect of demarcating resistance R 4, R5 is to be connected between 15,16 pins and power supply of detection chip as pull-up resistor.The effect of capacitor C 7, C8 is used for filtering and shielding, and prevents electromagnetic interference (EMI).Detection chip U3 is a high-precision impedance measurement chip, and inside is integrated with 12, and sampling rate is up to the frequency generator of the AD converter of 1MSPS.Its effect is that the driving voltage that produces characteristic frequency encourages element under test, the response signal obtained is sampled by ADC, and carry out discrete Fourier transform by the DSP on sheet, return to the value of real part and the imaginary values that obtain after Fourier transform under this output frequency, and result is sent into to microcontroller.15,16 pins are connected with+5V power supply by pull-up resistor R4, R5,12,13,14 pin earths, 9,10,11 pin parallel connections are connected with+5V power supply, 4, between 5 pins by toggle switch with demarcate resistance and be connected, 5,6 be used for reception survey element.
Testing process of the present invention is: at first, immunosensor is put into to the substrate solution that does not add agricultural chemicals, recorded its resistance value A1; Afterwards immunosensor is taken out, with after distilled water flushing, put it into liquid to be detected, wait for that 20 to 30 minutes so that it fully reacts, and records resistance value A2; Finally by the trace routine of moving on microcontroller, automatically calculate changing value, contrast impedance rate of change and pesticide concentration relation curve draw the pesticide concentration sizes values.The rate of change computing formula is as follows:
Rate of change=| A1-A2|/A1
If usually detect in solution and contain agricultural chemicals, will with immunosensor electrode generation specific immune response, cause reacted immunosensor resistance value to increase.The difference of A1 and A2 is larger, and the residues of pesticides concentration that shows to detect in solution is higher, and the difference of A1 and A2 is less, shows that the concentration of residues of pesticides is lower.
Impedance rate of change and pesticide concentration concern typical curve, can measure and draw respectively the liquid to be measured of many groups variable concentrations agricultural chemicals by electric impedance analyzer.
Microcontroller is made qualitative conclusion according to the impedance rate of change contrast standard curve obtained, and result is delivered to the display screen demonstration.
Below with regard to the embodiment of trace routine, do and illustrate:
At first, immunosensor being put into to substrate solution soaks 20 to 30 minutes.
Then, opening power, it is system clock that microcontroller is set crystal oscillator, the relevant pin of configuration, and initialization system.Select suitable demarcation resistance, click start button, enter testing process.
After entering testing process, detection chip Engage of standard pattern, microprocessor controls sends instruction to detection chip, detection chip receives instruction, start to produce the driving voltage signal of assigned frequency in sheet on DDS module sheet, this signal can pass through a programmable gain amplifier, can realize the peak-to-peak value output of four different range by ride gain.The ADC module starts to receive response signal after the setting-up time period expires.In receiving course, at first the signal obtained from impedor enters the current/voltage switching amplifier, what closely follow later is a programmable gain amplifier, through the signal after programmable gain amplifier, by ADC, is sampled, and the data that sampling obtains send DSP to carry out Fourier transform.1024 points of each frequency sampling carry out Fourier transform, export its value of real part and imaginary values, and flow to microcontroller.After microcontroller receives, calculate mould value and the phase angle of this impedance signal according to set formula.Can repeatedly, to relative standard deviation<15%, get last measured value and be delivered to the display screen demonstration.Measuring process finishes for the first time.
After completing above-mentioned steps, immunosensor is taken out with after distilled water flushing and put into solution to be measured, after waiting for 20 to 30 minutes, click and start test button, enter the measurement procedure (identical with reaction pre-test flow process) after immune response, obtain secondary resistance value.Microcontroller is delivered to display screen by it and is shown.And calculating rate of change, the contrast standard curve obtains concentration of substrate information, delivers to equally display screen and shows.Complete testing process.
The immunosensor that the present invention uses can be general, and therefore, the present invention goes for Multiple Pesticides residue detection occasion.
Claims (4)
1. the impedance type instrument for detecting pesticide residue through based on immunosensor is characterized in that by testing circuit, immunosensor, microcontroller, button, display screen and electric power generating composition:
Described testing circuit is connected and composed by feed circuit, detection chip, toggle switch, demarcation resistance, immunosensor: feed circuit are connected with detection chip with microcontroller, adopt first the 5V feed circuit that obtain through the method for filtering voltage regulation again through transformer pressure-reducing; Chip is connected with demarcation resistance by toggle switch, according to the input measurement range parameter, by toggle switch, selects corresponding demarcation resistance, with this, adjusts measurement sensitivity; Under the control of microcontroller, chip produce driving voltage be applied to liquid generation specific immune response to be measured after immunosensor on and collect response signal, obtain its real part and imaginary part after DFT, and it outputed to microcontroller;
Move trace routine on described microcontroller, mould value and phase angle that mould value and phase calculation formula by the impedance real part that receives and imaginary part by impedance calculates impedance, draw actual resistance value again after calibration, with set react before resistance value compare, draw rate of change, the contrast standard curve is reached a conclusion, and delivers to display screen and shows;
Described display screen is connected with microcontroller, for showing data and the window that carries out interactive operation as the user;
Described button is connected with testing circuit with microcontroller, for inputting dependent instruction and detected parameters;
Described power supply is connected with feed circuit.
2. according to the impedance type instrument for detecting pesticide residue through based on immunosensor of claim 1, it is characterized in that described detection chip adopts the AD5933 chip.
3. according to the impedance type instrument for detecting pesticide residue through based on immunosensor of claim 1, it is characterized in that the trace routine of moving on described microcontroller.
4. according to the impedance type instrument for detecting pesticide residue through based on immunosensor of claim 1, be characterised in that described microcontroller selects the STC89C52 microcontroller.
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Cited By (5)
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| CN103884764A (en) * | 2014-04-10 | 2014-06-25 | 山东理工大学 | Fast detector capable of detecting antibiotics and pesticide residue in milk simultaneously |
| CN105424770A (en) * | 2015-11-10 | 2016-03-23 | 中国科学院电子学研究所 | Electrochemical detector for detecting direct electrochemical immunity sensor |
| CN105866355A (en) * | 2016-04-14 | 2016-08-17 | 山东省农业科学院农业质量标准与检测技术研究所 | Multi-residual-pesticide multi-direction detector capable of wirelessly transmitting data |
| CN104965081B (en) * | 2015-05-29 | 2016-08-17 | 刘晓竹 | Antibody antigen detection method based on mobile device |
| CN113740384A (en) * | 2021-09-03 | 2021-12-03 | 重庆微奥云芯生物技术有限公司 | Molecular detection method |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103884764A (en) * | 2014-04-10 | 2014-06-25 | 山东理工大学 | Fast detector capable of detecting antibiotics and pesticide residue in milk simultaneously |
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| CN105866355B (en) * | 2016-04-14 | 2018-02-13 | 山东省农业科学院农业质量标准与检测技术研究所 | It is a kind of can Wireless data transmission data the multi-direction detector of multi-tesidual pesticides |
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| CN113740384B (en) * | 2021-09-03 | 2024-01-09 | 重庆微奥云芯生物技术有限公司 | Molecular detection method |
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