CN110132929A - A kind of food safety time-resolved fluorescence quick and quantitative determination system - Google Patents
A kind of food safety time-resolved fluorescence quick and quantitative determination system Download PDFInfo
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- CN110132929A CN110132929A CN201910503967.4A CN201910503967A CN110132929A CN 110132929 A CN110132929 A CN 110132929A CN 201910503967 A CN201910503967 A CN 201910503967A CN 110132929 A CN110132929 A CN 110132929A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
A kind of food safety time-resolved fluorescence quick and quantitative determination system, including fluorogenic quantitative detection test strips, quantitative fluorescence analysis instrument and data are traced to the source cloud management platform；Sample pad, bonding pad, nitrocellulose filter and water absorption pad are arranged on substrate；Main detection excitation module output end in excitation light path module receives the main detection photoelectric conversion module in conversion module with photosignal and connect, and the output end of the auxiliary optical scan module in excitation light path module is connect with the input terminal that photosignal receives the sub-scanning photoelectric conversion module in conversion module；The output end of sub-scanning photoelectric conversion module is connected by amplification and rectification circuit and decoding interface circuit and the input terminal of display device.The present invention has sample pre-treatments simple, marker preparation convenience, detection time end, high sensitivity, high specificity, storage time long, "dead" pollution, detect it is reproducible and the advantages of have a wide range of application.
Description
Technical field
The present invention relates to field of food detection more particularly to a kind of food safety time-resolved fluorescence rapid quantitative detection systems
System.
Background technique
Time-resolved fluoroimmunoassay chromatographic theory: to be measured when the sample drop of determined antigen (antibody) will be contained in sample application zone
The antibody (antigen) of antigen (antibody) in sample and the label of the fluorescent nanometer microsphere in bonding pad combines and through capillary action
It chromatographs forward, after reaching detection zone, is combined with antibody (antigen) fixed in detection line, it is anti-to form microparticle-antibody-antigen-
Body sandwich complex is simultaneously fixed in detection line, and the continuation of extra Fluorescent microsphere marker chromatographs forward, and is fixed on matter
Control two anti-binding of line.After reaction, glimmering in detection line and nature controlling line with ultraviolet source (340nm) to detection zone Scanning Detction
Light nanosphere issues high-intensitive fluorescence (615nm), and decay time is also longer.Using time of measuring is delayed, to sample base
After abiogenous short life fluorescence (1-10ns) is all decayed in matter, then the specificity fluorescent of rare earth element is measured, thus
The interference of special background fluorescence can be excluded completely.Pass through the power and its ratio of detection line and nature controlling line fluorescence intensity
Analyze the concentration of determinand in sample.And existing colloid gold immune analytic approach and the unstability of enzyme-linked immunosorbent assay,
Chemiluminescence immune assay is complicated vulnerable to environmental disturbances and Electrogenerated chemiluminescent immunoassay selling at exorbitant prices, chromatography or mass spectrum operation
And it is bothersome and the disadvantages of high is required to testing staff, to solve the above problems, proposing a kind of food safety time point in the application
Distinguish fluorescence quick and quantitative determination system.
Summary of the invention
(1) goal of the invention
To solve technical problem present in background technique, the present invention proposes a kind of food safety time-resolved fluorescence fast quantification
Detection system, with sample pre-treatments, simple, marker prepares convenience, detection time end, high sensitivity, high specificity, storage
Time is long, and "dead" pollution, detection are reproducible and the advantages of have a wide range of application.
(2) technical solution
To solve the above problems, the present invention provides a kind of food safety time-resolved fluorescence quick and quantitative determination systems, including
Fluorogenic quantitative detection test strips, quantitative fluorescence analysis instrument and data are traced to the source cloud management platform；Fluorogenic quantitative detection test strips include
Substrate, sample pad, bonding pad, nitrocellulose filter, T line, C line and water absorption pad；Sample pad, bonding pad, nitrocellulose filter and
Water absorption pad is arranged on substrate；Nitrocellulose filter is equipped with T line and C line, bonding pad are positioned close to nitrocellulose filter
On T line side, and be overlapped on cellulose nitrate speed film；Water absorption pad is positioned close to one of the C line on nitrocellulose filter
Side, and be overlapped on cellulose nitrate speed film, side of the bonding pad far from nitrocellulose filter is arranged in sample pad, and is overlapped on knot
It closes on pad；Bar code area is provided on substrate；
Quantitative fluorescence analysis instrument receives conversion module, control system mould by mechanical driving device, excitation light path module, photosignal
Block, data cache module and display device composition；Excitation light path module includes main detection excitation module and auxiliary optical scanning
Module；The main detection excitation module includes the first excitation light source, the first lens, the first optical filter, the second lens, light splitting
Mirror, the second optical filter, the third lens and grating；The auxiliary optical scan module include the second excitation light source, third optical filter,
4th lens and the 5th lens；It includes that main detection photoelectric conversion module and sub-scanning photoelectricity turn that photosignal, which receives conversion module,
Change the mold block；Control system module receives conversion module, data with mechanical driving device, excitation light path module, photosignal respectively
Cache module is connected with display device；Main detection excitation module output end and photosignal in excitation light path module, which receive, to be turned
Change the mold the main detection photoelectric conversion module connection in block, and the output end connection and display device of main detection photoelectric conversion module
Input terminal connection；The output end and photosignal of auxiliary optical scan module in excitation light path module receive in conversion module
The input terminal of sub-scanning photoelectric conversion module connects；The output end of sub-scanning photoelectric conversion module passes through amplification and rectification circuit
It is connected with the input terminal of decoding interface circuit and display device, sub-scanning photoelectric conversion module is connect with data cache module；
Fluorogenic quantitative detection test strips are arranged on mechanical driving device；
The trace to the source input terminal of cloud management platform of the output end of data cache module and data is connect.
Preferably, sample pad 2 is water adsorption glass tunica fibrosa.
Preferably, water absorption pad 7 is blotting paper.
Preferably, substrate 1 is polyvinyl chloride plastic sheets.
Preferably, display device includes liquid crystal display and printing equipment.
Above-mentioned technical proposal of the invention has following beneficial technical effect: control system module controls the first exciting light
The light that source issues is emitted to the inspection of fluorogenic quantitative detection test strips through the first lens, the first optical filter, spectroscope and the second lens
It surveys in area, the phosphorescent signal that detection zone issues is mapped to main detection photoelectric conversion module through the second optical filter, the third lens and grating,
It is input in display device through photoelectric conversion；Control system module controls the second excitation light source and emits excitation beam, filters through third
Mating plate, the 4th lens are emitted to the bar code area 8 of fluorogenic quantitative detection test strips, and bar code area 8 reflects laser signal again through the 5th
Lens, sub-scanning photoelectric conversion module, amplification and rectification circuit and decoding interface circuit are input in display device, meanwhile, and
By the storage of the data of analysis to in data cache module, data cache module upload the data to data and traces to the source cloud management platform
On, then, the present invention has that sample pre-treatments are simple, marker preparation convenience, detection time end, high sensitivity, specificity
By force, storage time is long, and "dead" pollution, detection are reproducible and the advantages of have a wide range of application.
Detailed description of the invention
Fig. 1 is a kind of structural representation of food safety time-resolved fluorescence quick and quantitative determination system proposed by the present invention
Figure.
Fig. 2 is fluorescent quantitation point in a kind of food safety time-resolved fluorescence quick and quantitative determination system proposed by the present invention
The structural schematic diagram of analyzer.
Appended drawing reference:
1, substrate；2, sample pad；3, bonding pad；4, nitrocellulose filter；5, T line；6, C line；7, water absorption pad；8, bar code area.
Specific embodiment
In order to make the objectives, technical solutions and advantages of the present invention clearer, With reference to embodiment and join
According to attached drawing, the present invention is described in more detail.It should be understood that these descriptions are merely illustrative, and it is not intended to limit this hair
Bright range.In addition, in the following description, descriptions of well-known structures and technologies are omitted, to avoid this is unnecessarily obscured
The concept of invention.
As illustrated in fig. 1 and 2, a kind of food safety time-resolved fluorescence quick and quantitative determination system proposed by the present invention, packet
Fluorogenic quantitative detection test strips, quantitative fluorescence analysis instrument and data are included to trace to the source cloud management platform；Fluorogenic quantitative detection test strips packet
Include substrate 1, sample pad 2, bonding pad 3, nitrocellulose filter 4, T line 5, C line 6 and water absorption pad 7；Sample pad 2, bonding pad 3, nitric acid
Cellulose membrane 4 and water absorption pad 7 are respectively provided on substrate 1；Nitrocellulose filter 4 is equipped with T line 5 and C line 6, and the setting of bonding pad 3 exists
The side of T line 5 on nitrocellulose filter 4, and be overlapped on cellulose nitrate speed film 4；Water absorption pad 7 is positioned close to nitric acid
The side of C line 6 on cellulose membrane 4, and be overlapped on cellulose nitrate speed film 4, sample pad 2 is arranged in bonding pad 3 far from nitric acid
The side of cellulose membrane 4, and be overlapped on bonding pad 3；Bar code area 8 is provided on substrate 1；
Quantitative fluorescence analysis instrument receives conversion module, control system mould by mechanical driving device, excitation light path module, photosignal
Block, data cache module and display device composition；Excitation light path module includes main detection excitation module and auxiliary optical scanning
Module；The main detection excitation module includes the first excitation light source, the first lens, the first optical filter, the second lens, light splitting
Mirror, the second optical filter, the third lens and grating；The auxiliary optical scan module include the second excitation light source, third optical filter,
4th lens and the 5th lens；It includes that main detection photoelectric conversion module and sub-scanning photoelectricity turn that photosignal, which receives conversion module,
Change the mold block；Control system module receives conversion module, data with mechanical driving device, excitation light path module, photosignal respectively
Cache module is connected with display device；Main detection excitation module output end and photosignal in excitation light path module, which receive, to be turned
Change the mold the main detection photoelectric conversion module connection in block, and the output end connection and display device of main detection photoelectric conversion module
Input terminal connection；The output end and photosignal of auxiliary optical scan module in excitation light path module receive in conversion module
The input terminal of sub-scanning photoelectric conversion module connects；The output end of sub-scanning photoelectric conversion module passes through amplification and rectification circuit
It is connected with the input terminal of decoding interface circuit and display device, sub-scanning photoelectric conversion module is connect with data cache module；
Fluorogenic quantitative detection test strips are arranged on mechanical driving device；
The trace to the source input terminal of cloud management platform of the output end of data cache module and data is connect.
In the present invention, control system module control the first excitation light source issue light through the first lens, the first optical filter, point
Light microscopic and the second lens are emitted in the detection zone of fluorogenic quantitative detection test strips, and the phosphorescent signal that detection zone issues is through the second filter
Mating plate, the third lens and grating are mapped to main detection photoelectric conversion module, are input in display device through photoelectric conversion；Control system
Module controls the second excitation light source and emits excitation beam, is emitted to fluorogenic quantitative detection test paper through third optical filter, the 4th lens
The bar code area 8 of item, bar code area 8 reflect laser signal again through the 5th lens, sub-scanning photoelectric conversion module, amplification shaping electricity
Road and decoding interface circuit are input in display device, meanwhile, and by the storage of the data of analysis to in data cache module, number
Data are upload the data to according to cache module to trace to the source on cloud management platform.
In an alternative embodiment, sample pad 2 is water adsorption glass tunica fibrosa.
In an alternative embodiment, water absorption pad 7 is blotting paper.
In an alternative embodiment, substrate 1 is polyvinyl chloride plastic sheets.
In an alternative embodiment, display device includes liquid crystal display and printing equipment.
It should be understood that above-mentioned specific embodiment of the invention is used only for exemplary illustration or explains of the invention
Principle, but not to limit the present invention.Therefore, that is done without departing from the spirit and scope of the present invention is any
Modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.In addition, appended claims purport of the present invention
Covering the whole variations fallen into attached claim scope and boundary or this range and the equivalent form on boundary and is repairing
Change example.
Claims (5)
1. a kind of food safety time-resolved fluorescence quick and quantitative determination system, which is characterized in that tried including fluorogenic quantitative detection
Paper slip, quantitative fluorescence analysis instrument and data are traced to the source cloud management platform；Fluorogenic quantitative detection test strips include substrate (1), sample pad
(2), bonding pad (3), nitrocellulose filter (4), T line (5), C line (6) and water absorption pad (7)；Sample pad (2), bonding pad (3), nitre
Acid cellulose film (4) and water absorption pad (7) are arranged on substrate (1)；Nitrocellulose filter (4) is equipped with T line (5) and C line
(6), bonding pad (3) is positioned close to the side of the T line (5) on nitrocellulose filter (4), and is overlapped on cellulose nitrate speed film
(4) on；Water absorption pad (7) is positioned close to the side of the C line (6) on nitrocellulose filter (4), and is overlapped on cellulose nitrate speed film
(4) on, sample pad (2) setting is overlapped on bonding pad (3) in the side of bonding pad (3) far from nitrocellulose filter (4)；
Bar code area (8) are provided on substrate (1)；
Quantitative fluorescence analysis instrument receives conversion module, control system mould by mechanical driving device, excitation light path module, photosignal
Block, data cache module and display device composition；Excitation light path module includes main detection excitation module and auxiliary optical scanning
Module；The main detection excitation module includes the first excitation light source, the first lens, the first optical filter, the second lens, light splitting
Mirror, the second optical filter, the third lens and grating；The auxiliary optical scan module include the second excitation light source, third optical filter,
4th lens and the 5th lens；It includes that main detection photoelectric conversion module and sub-scanning photoelectricity turn that photosignal, which receives conversion module,
Change the mold block；Control system module receives conversion module, data with mechanical driving device, excitation light path module, photosignal respectively
Cache module is connected with display device；Main detection excitation module output end and photosignal in excitation light path module, which receive, to be turned
Change the mold the main detection photoelectric conversion module connection in block, and the output end connection and display device of main detection photoelectric conversion module
Input terminal connection；The output end and photosignal of auxiliary optical scan module in excitation light path module receive in conversion module
The input terminal of sub-scanning photoelectric conversion module connects；The output end of sub-scanning photoelectric conversion module passes through amplification and rectification circuit
It is connected with the input terminal of decoding interface circuit and display device, sub-scanning photoelectric conversion module is connect with data cache module；
Fluorogenic quantitative detection test strips are arranged on mechanical driving device；The output end of data cache module and data cloud management of tracing to the source are flat
The input terminal of platform connects.
2. a kind of food safety time-resolved fluorescence quick and quantitative determination system according to claim 1, which is characterized in that
Sample pad (2) is water adsorption glass tunica fibrosa.
3. a kind of food safety time-resolved fluorescence quick and quantitative determination system according to claim 1, which is characterized in that
Water absorption pad (7) is blotting paper.
4. a kind of food safety time-resolved fluorescence quick and quantitative determination system according to claim 1, which is characterized in that
Substrate (1) is polyvinyl chloride plastic sheets.
5. a kind of food safety time-resolved fluorescence quick and quantitative determination system according to claim 1, which is characterized in that
Display device includes liquid crystal display and printing equipment.
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| CN201910503967.4A CN110132929A (en) | 2019-06-12 | 2019-06-12 | A kind of food safety time-resolved fluorescence quick and quantitative determination system |
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| CN201910503967.4A CN110132929A (en) | 2019-06-12 | 2019-06-12 | A kind of food safety time-resolved fluorescence quick and quantitative determination system |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101614748A (en) * | 2008-06-25 | 2009-12-30 | 上海新波生物技术有限公司 | Follicle-stimulating hormone time-resolved fluoroimmunoassay method and kit |
| CN102866135A (en) * | 2012-06-27 | 2013-01-09 | 深圳市艾瑞生物科技有限公司 | Time resolution fluorescent biosensor based on phosphorescent light emitting technology and application thereof |
| CN109406778A (en) * | 2018-10-15 | 2019-03-01 | 国家烟草质量监督检验中心 | The time-resolved fluorescence quantitative test paper item and its preparation method and application of ralstonia solanacearum in a kind of detection tobacco leaf |
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2019
- 2019-06-12 CN CN201910503967.4A patent/CN110132929A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101614748A (en) * | 2008-06-25 | 2009-12-30 | 上海新波生物技术有限公司 | Follicle-stimulating hormone time-resolved fluoroimmunoassay method and kit |
| CN102866135A (en) * | 2012-06-27 | 2013-01-09 | 深圳市艾瑞生物科技有限公司 | Time resolution fluorescent biosensor based on phosphorescent light emitting technology and application thereof |
| CN109406778A (en) * | 2018-10-15 | 2019-03-01 | 国家烟草质量监督检验中心 | The time-resolved fluorescence quantitative test paper item and its preparation method and application of ralstonia solanacearum in a kind of detection tobacco leaf |
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