CN110122084A - Cuttage breeding method in a kind of Morinda officinalis tissue-cultured seedling room - Google Patents
Cuttage breeding method in a kind of Morinda officinalis tissue-cultured seedling room Download PDFInfo
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- CN110122084A CN110122084A CN201910435615.XA CN201910435615A CN110122084A CN 110122084 A CN110122084 A CN 110122084A CN 201910435615 A CN201910435615 A CN 201910435615A CN 110122084 A CN110122084 A CN 110122084A
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- seedling
- morinda officinalis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/02—Treatment of plants with carbon dioxide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
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- Developmental Biology & Embryology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to Morinda officinalis tissue-cultured seedling reproduction technique fields, and in particular to cuttage breeding method in a kind of Morinda officinalis tissue-cultured seedling room.The present invention adds nutrient solution using indoor cottage method, in cuttage seed plate, promotes seedling fast-growth；It is passed through CO2And extend the illumination cultivation time, by reinforcing photosynthesis, promote cutting autotrophy, germination takes root, the seedling well developed root system of culture, can directly transplanting survival rate it is high, growth is rapid.Material therefor, utensil, culture solution, operating process and culture environment of the present invention etc. keep clean state, and pollution rate is very low, high production efficiency；Have many advantages, such as that nursery speed is fast, it is with short production cycle, seedling is healthy and strong, well developed root system, high-quality, direct transplanting survival rate is high, growth is rapid.
Description
Technical field
The invention belongs to Morinda officinalis tissue-cultured seedling reproduction technique fields, and in particular to cuttage is numerous in a kind of Morinda officinalis tissue-cultured seedling room
Grow method.
Background technique
Morinda officinalis is perennial woody climber medicinal plant, is used as medicine with fleshy root, has kidney-replenishing, strengthening the bones and muscles, wind-damp dispelling
The effect of.In the production of Morinda officinalis artificial cultivation, seeling industry is carried out based on cutting propagation, long-term vegetative propagation will lead to product
Kind is degenerated, disease accumulation, and the seedling quality of production is poor.In recent years, virus-free purification and rejuvenation culture is carried out by tissue culture technology, obtained
Morinda officinalis high quality seedling is producing upper Preliminary Applications.But according to current Morinda officinalis tissue-cultured seedling production technology, tissue-cultured seedling is in life
When root strong seedling culture, base portion root of hair easily forms callus compared with slow, radical is less, and when washing seedling, root is easy to fall off, and tissue-cultured seedling moves
Slow growth after cultivation, it is difficult to promote and apply.Morinda officinalis tissue culture seedling direct transplantation is slow in the presence of growth at present, uniformity is poor, nursery is all
The problem that phase is long, production efficiency is low.
Summary of the invention
The present invention provides cuttage breeding method in a kind of Morinda officinalis tissue-cultured seedling room, overcomes defect of the existing technology, tool
Have nursery speed fast, it is with short production cycle, seedling is healthy and strong, well developed root system, high-quality, direct transplanting survival rate is high, growth rapidly etc.
Advantage.
To achieve the above object, the present invention adopts the following technical scheme:
Cuttage breeding method in a kind of Morinda officinalis tissue-cultured seedling room, comprising the following steps:
(1) it cauline leaf stalwartness tissue-cultured seedling culture: is obtained a large amount of using conventional plant tissue culture technology by induction differentiation culture
Multiple Buds plant division is accessed strong seedling culture base MS+0.2-1.0 mg.L by Morinda officinalis Multiple Buds-1It is cultivated on NAA, it is strong to obtain cauline leaf
Strong Morinda officinalis tissue-cultured seedling；
(2) preparation with leaf cuttage item: Morinda officinalis tissue-cultured seedling being taken out, shearing, 1-3 stipes of each cutting band, 2-6 leaves
Piece, stem length 1-3cm, of short duration bubble are spare in clean water；
(3) cuttage seed plate adds nutrient solution: loose, ventilative matrix is added in seedlings nursing plate, surface smoothes out, be added without it is organic at
Divide the nutrient solution with sugar, with the anhydrous exudation of stromal surface to spend, nutrient composition is 0.5-1.0 MS+0-0.5 mg.L-1
NAA；
(4) with the cuttage of sectional leaf cutting: cutting being inserted into the matrix of seedlings nursing plate, depth 1-2 cm, the not phase mutual respect of the blade of cutting
It is folded, carbendazim or chlorothalonil are sprayed after cuttage, and seedlings nursing plate is placed in equipped with illumination, humidity generator, CO2Steel cylinder and control
It is cultivated in the closed subenvironment or incubator of device processed；
(5) it is passed through CO2And reinforce illumination cultivation: the humidity of cutting culture environment is maintained at 85% or more, and cultivation temperature is 26 ± 2
DEG C, it is passed through the CO of 800-1200 ppm daily2, daily light application time 16-20 h, light intensity 2000-5000 Lux are passed through CO2When
Between it is synchronous with light application time；
(6) seedling is bred as transplanting: by 30-60 days Rooting and hardening-off cultures, Morinda officinalis tissue-cultured seedling cutting sprouting grew 5-10cm,
Young leaves growth 4-8 piece, plant height 6-15cm, well developed root system, root carrying substrates are directly transplanted, 95% or more survival rate, and seedling growth is fast
Speed；
Wherein material therefor, utensil, culture solution, operating process and culture environment etc. are required to keep clean in (1)-(6)
Net state.
Compared with traditional tissue culture propagation and cutting propagation, remarkable advantage of the invention is:
1. the present invention adds nutrient solution in cuttage seed plate, promote seedling fast-growth；
2. the present invention is passed through CO2And extend the illumination cultivation time.By reinforcing photosynthesis, promote cutting autotrophy, germination life
Root, the seedling well developed root system of culture, can directly transplanting survival rate it is high, growth is rapid.
3. the present invention is using indoor cuttage.Cuttage work is carried out in the environment of manual control, round-the-clock can be produced, by
Clean state is kept in material therefor, utensil, culture solution, operating process and culture environment etc., pollution rate is very low, production efficiency
It is high.
Detailed description of the invention
Cuttage culture situation (culture 60 days) in Fig. 1 Morinda officinalis tissue-cultured seedling room；
60 days seedling growing states of cuttage culture in Fig. 2 Morinda officinalis tissue-cultured seedling room.
Specific embodiment
Further to disclose rather than the present invention is limited, the present invention is described in further detail below in conjunction with example.
Embodiment 1
Cuttage breeding method in a kind of Morinda officinalis tissue-cultured seedling room, the specific steps are that:
(1) it cauline leaf stalwartness tissue-cultured seedling culture: is obtained a large amount of using conventional plant tissue culture technology by induction differentiation culture
Multiple Buds plant division is accessed strong seedling culture base MS+0.2mg.L by Morinda officinalis Multiple Buds-1It is cultivated on NAA, obtains cauline leaf stalwartness
Morinda officinalis tissue-cultured seedling；
(2) preparation with leaf cuttage item: Morinda officinalis tissue-cultured seedling is taken out, shearing, 1 stipes of each cutting band, 2 blades, stem
Dry length 1-2cm, of short duration bubble are spare in clean water；
(3) cuttage seed plate adds nutrient solution: loose, ventilative matrix is added in seedlings nursing plate, surface smoothes out, be added without it is organic at
Divide the nutrient solution with sugar, with the anhydrous exudation of stromal surface to spend, nutrient composition is+0.5 mg.L of 1.0 MS-1NAA；
(4) with the cuttage of sectional leaf cutting: by the matrix of cutting insertion seedlings nursing plate, 1 cm of depth, the blade of cutting is not overlapped,
The fungicide such as carbendazim or Bravo are sprayed after cuttage, and seedlings nursing plate is placed in equipped with illumination, humidity generator, CO2Steel cylinder and control
It is cultivated in the closed subenvironment or incubator of device processed；
(5) it is passed through CO2And reinforce illumination cultivation: the humidity of cutting culture environment is maintained at 90%, and cultivation temperature is 26 ± 2 DEG C,
It is passed through the CO of 800 ppm daily2, daily 16 h of light application time, 3000 Lux of light intensity are passed through CO2Time and light application time it is same
Step；
(6) seedling is bred as transplanting: by 45 days Rooting and hardening-off cultures, Morinda officinalis tissue-cultured seedling cutting sprouting grew 5-8cm, young leaves
Growth 4-6 piece, plant height 6-12cm, well developed root system, root carrying substrates are directly transplanted, survival rate 95.5%, and seedling growth is rapid；
Wherein material therefor, utensil, culture solution, operating process and culture environment etc. are required to keep clean in (1)-(6)
Net state.
Embodiment 2
Cuttage breeding method in a kind of Morinda officinalis tissue-cultured seedling room, the specific steps are that:
(1) it cauline leaf stalwartness tissue-cultured seedling culture: is obtained a large amount of using conventional plant tissue culture technology by induction differentiation culture
Multiple Buds plant division is accessed+1.0 mg.L of strong seedling culture base MS by Morinda officinalis Multiple Buds-1It is cultivated on NAA, obtains cauline leaf stalwartness
Morinda officinalis tissue-cultured seedling；
(2) preparation with leaf cuttage item: Morinda officinalis tissue-cultured seedling is taken out, shearing, 2 stipes of each cutting band, 4 blades, stem
Dry length 1.5-3cm, of short duration bubble are spare in clean water；
(3) cuttage seed plate adds nutrient solution: loose, ventilative matrix is added in seedlings nursing plate, surface smoothes out, be added without it is organic at
Divide the nutrient solution with sugar, with the anhydrous exudation of stromal surface to spend, nutrient composition is+0.25 mg.L of 0.75 MS-1NAA；
(4) with the cuttage of sectional leaf cutting: by the matrix of cutting insertion seedlings nursing plate, the blade of depth 1cm, cutting are not overlapped,
The fungicide such as carbendazim or Bravo are sprayed after cuttage, and seedlings nursing plate is placed in equipped with illumination, humidity generator, CO2Steel cylinder and control
It is cultivated in the closed subenvironment or incubator of device processed；
(5) it is passed through CO2And reinforce illumination cultivation: the humidity of cutting culture environment is maintained at 95%, and cultivation temperature is 26 ± 2 DEG C,
It is passed through the CO of 1200 ppm daily2, daily 18 h of light application time, 4000 Lux of light intensity are passed through CO2Time and light application time it is same
Step；
(6) seedling is bred as transplanting: by 50 days Rooting and hardening-off cultures, Morinda officinalis tissue-cultured seedling cutting sprouting grew 5-10cm, newly
Leaf growth 2-6 piece, plant height 7-12cm, well developed root system, root carrying substrates are directly transplanted, survival rate 98.0%, and seedling growth is rapid；
Wherein material therefor, utensil, culture solution, operating process and culture environment etc. are required to keep clean in (1)-(6)
Net state.
Embodiment 3
Cuttage breeding method in a kind of Morinda officinalis tissue-cultured seedling room, the specific steps are that:
(1) it cauline leaf stalwartness tissue-cultured seedling culture: is obtained a large amount of using conventional plant tissue culture technology by induction differentiation culture
Multiple Buds plant division is accessed+0.5 mg.L of strong seedling culture base MS by Morinda officinalis Multiple Buds-1It is cultivated on NAA, obtains cauline leaf stalwartness
Morinda officinalis tissue-cultured seedling；
(2) preparation with leaf cuttage item: Morinda officinalis tissue-cultured seedling being taken out, shearing, 2-3 stipes of each cutting band, 4-6 leaves
Piece, stem length 1.5-3cm, of short duration bubble are spare in clean water；
(3) cuttage seed plate adds nutrient solution: loose, ventilative matrix is added in seedlings nursing plate, surface smoothes out, be added without it is organic at
Divide the nutrient solution with sugar, with the anhydrous exudation of stromal surface to spend, nutrient composition is+0.1 mg.L of 0.5 MS-1NAA；
(4) with the cuttage of sectional leaf cutting: by the matrix of cutting insertion seedlings nursing plate, 2 cm of depth, the blade of cutting is not overlapped,
The fungicide such as carbendazim or Bravo are sprayed after cuttage, and seedlings nursing plate is placed in equipped with illumination, humidity generator, CO2Steel cylinder and control
It is cultivated in the closed subenvironment or incubator of device processed；
(5) it is passed through CO2And reinforce illumination cultivation: the humidity of cutting culture environment is maintained at 92%, and cultivation temperature is 26 ± 2 DEG C,
It is passed through the CO of 1200 ppm daily2, extend light application time, daily 20 h of light application time, 4500 Lux of light intensity are passed through CO2Time
It is synchronous with light application time；
(6) seedling is bred as transplanting: by 60 days Rooting and hardening-off cultures, Morinda officinalis tissue-cultured seedling cutting sprouting grew 8-15cm, newly
Leaf growth 4-8 piece, plant height 10-15cm, well developed root system, root carrying substrates are directly transplanted, survival rate 99.0%, and seedling growth is rapid；
Wherein material therefor, utensil, culture solution, operating process and culture environment etc. are required to keep clean in (1)-(6)
Net state.
Comparative example 1
The breeding of Morinda officinalis tissue culture seedling direct transplantation, the specific steps are that:
(1) it cauline leaf stalwartness tissue-cultured seedling culture: is obtained a large amount of using conventional plant tissue culture technology by induction differentiation culture
Multiple Buds plant division is accessed+0.5 mg.L of strong seedling culture base MS by Morinda officinalis Multiple Buds-1It is cultivated on NAA, obtains cauline leaf stalwartness
Morinda officinalis tissue-cultured seedling, hardening of uncapping 5 days；
(2) tissue culture seedling direct transplantation: the Morinda officinalis tissue-cultured seedling after hardening is taken out, and carefully washes away culture medium, retains root system, directly
It is transplanted to seedbed, the matrix in seedbed wants loose ventilative water conservation, and the fungicide such as carbendazim or Bravo are sprayed after cultivation；
(3) manage after tissue culture transplantation of seedlings: after tissue culture transplantation of seedlings, seedbed covers film, shades above, light transmittance 30%-50%, humidity
It is maintained at 85% or more, seedling growth is slower, and training orientation 60 days, survival rate 55.0%.
Comparative example 2
Morinda officinalis tissue-cultured seedling cuttage culture transplanting breeding, the specific steps are that:
(1) it cauline leaf stalwartness tissue-cultured seedling culture: is obtained a large amount of using conventional plant tissue culture technology by induction differentiation culture
Multiple Buds plant division is accessed+0.5 mg.L of strong seedling culture base MS by Morinda officinalis Multiple Buds-1It is cultivated on NAA, obtains cauline leaf stalwartness
Morinda officinalis tissue-cultured seedling；
(2) preparation with leaf cuttage item: Morinda officinalis tissue-cultured seedling being taken out, shearing, 1-3 stipes of each cutting band, 2-6 leaves
Piece, stem length 1-3cm, of short duration bubble are spare in clean water；
(3) with the cuttage of sectional leaf cutting: loose, ventilative matrix being added in seedlings nursing plate, surface smoothes out.By cutting insertion seedlings nursing plate
In matrix, 1.5 cm of depth, the blade of cutting is not overlapped, and the fungicide such as carbendazim or Bravo is sprayed after cuttage, by nursery
Disk is placed in be cultivated equipped in illumination, humidity generator subenvironment or incubator,
(4) illumination cultivation: the humidity of cutting culture environment is maintained at 85% or more, and cultivation temperature is 26 ± 2 DEG C, when daily illumination
18 h, 4000 Lux of light intensity；
(5) seedling is bred as transplanting: by 60 days Rooting and hardening-off cultures, Morinda officinalis tissue-cultured seedling cutting sprouting grew 0.5-2cm, raw
Long slow, plant height 3-7 cm is directly transplanted, survival rate 61.5%.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (4)
1. cuttage breeding method in a kind of Morinda officinalis tissue-cultured seedling room, it is characterised in that: the following steps are included:
(1) it cauline leaf stalwartness tissue-cultured seedling culture: is obtained a large amount of using conventional plant tissue culture technology by induction differentiation culture
Multiple Buds plant division is accessed strong seedling culture base MS+0.2-1.0 mg.L by Morinda officinalis Multiple Buds-1It is cultivated on NAA, it is strong to obtain cauline leaf
Strong Morinda officinalis tissue-cultured seedling；
(2) preparation with leaf cuttage item: Morinda officinalis tissue-cultured seedling being taken out, shearing, 1-3 stipes of each cutting band, 2-6 leaves
Piece, stem length 1-3cm, of short duration bubble are spare in clean water；
(3) cuttage seed plate adds nutrient solution: loose, ventilative matrix is added in seedlings nursing plate, surface smoothes out, be added without it is organic at
Divide the nutrient solution with sugar, is to spend with the anhydrous exudation of stromal surface；
(4) with the cuttage of sectional leaf cutting: cutting being inserted into the matrix of seedlings nursing plate, depth 1-2 cm, the not phase mutual respect of the blade of cutting
It is folded, fungicide carbendazim or Bravo are sprayed after cuttage, and seedlings nursing plate is placed in equipped with illumination, humidity generator, CO2Steel cylinder and control
It is cultivated in the closed subenvironment or incubator of device processed；
(5) it is passed through CO2And reinforce illumination cultivation: the humidity of cutting culture environment is maintained at 85% or more, and cultivation temperature is 26 ± 2
DEG C, it is passed through the CO of 800-1200 ppm daily2, extend light application time, be passed through CO2Time it is synchronous with light application time；
(6) seedling is bred as transplanting: by 30-60 days Rooting and hardening-off cultures, Morinda officinalis tissue-cultured seedling cutting sprouting grew 5-10cm,
Young leaves growth 4-8 piece, plant height 6-15cm, well developed root system, root carrying substrates are directly transplanted, 95% or more survival rate, and seedling growth is fast
Speed.
2. a kind of Morinda officinalis tissue-cultured seedling cuttage breeding method according to claim 1, it is characterised in that: step (3) nutrition
Liquid ingredient is 0.5-1.0 MS+0-0.5 mg.L-1 NAA。
3. a kind of Morinda officinalis tissue-cultured seedling cuttage breeding method according to claim 1, it is characterised in that: step (5) is described
Light application time is specially daily light application time 16-20 h, light intensity 2000-5000 Lux.
4. a kind of Morinda officinalis tissue-cultured seedling cuttage breeding method according to claim 1, it is characterised in that: the step (1)-
(6) material therefor, utensil, culture solution, operating process and culture environment are required to keep clean state in.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111345163A (en) * | 2020-03-18 | 2020-06-30 | 吉林省林业科学研究院 | Rapid rooting method for tilia amurensis cutting slips |
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Application publication date: 20190816 |
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