CN110115267B - Compound composition for resisting diseases and killing pests - Google Patents

Compound composition for resisting diseases and killing pests Download PDF

Info

Publication number
CN110115267B
CN110115267B CN201910422806.2A CN201910422806A CN110115267B CN 110115267 B CN110115267 B CN 110115267B CN 201910422806 A CN201910422806 A CN 201910422806A CN 110115267 B CN110115267 B CN 110115267B
Authority
CN
China
Prior art keywords
chlorfenapyr
fluopyram
compound composition
agent
chive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201910422806.2A
Other languages
Chinese (zh)
Other versions
CN110115267A (en
Inventor
罗建军
翁群芳
胡琼波
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
South China Agricultural University
Original Assignee
South China Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by South China Agricultural University filed Critical South China Agricultural University
Priority to CN201910422806.2A priority Critical patent/CN110115267B/en
Publication of CN110115267A publication Critical patent/CN110115267A/en
Application granted granted Critical
Publication of CN110115267B publication Critical patent/CN110115267B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a compound composition for resisting diseases and killing insects, belonging to the technical field of pesticides. The active ingredients of the composition comprise fluopyram and chlorfenapyr, the weight ratio of the fluopyram to the chlorfenapyr is 1: 50-50: 1, and the fluopyram and the chlorfenapyr account for 5-60% of the weight of the composition. The compound composition disclosed by the invention achieves the effect of treating insect pests and diseases by one-time application through the compounding of the fluopyram and the chlorfenapyr, is time-saving and labor-saving, and particularly has a good effect of preventing and treating chive maggot and chive root nematode disease.

Description

Compound composition for resisting diseases and killing pests
Technical Field
The invention belongs to the technical field of pesticides, and relates to a compound composition for resisting diseases and killing insects.
Background
Nematodes belong to the phylum of the phylum nematoda, are tiny in shape and can only be observed with the aid of a microscope. About 3000 nematodes which are common and serious in China and harmful to plants live in soil mostly, and some nematodes are also parasitic in plants. Nematodes spread through soil or seeds and can damage the root system of plants. The leek root-knot nematodes only harm the root, invade from the root and eat the underground pseudostem and rhizome, and the secretion thereof can stimulate the cell proliferation and the expansion after entering the root tissue to form a plurality of nodose objects with different shapes and sizes, the root knots are milky white and solid, then gradually turn black and brown with the continuous increase of the harm, and are often rotten. Therefore, in a protected area with serious damage of root-knot nematodes, other diseases of the Chinese chives are easy to damage. As the roots of the upper part and the lower part of the ground are damaged by the root-knot nematodes, the activity of the roots is reduced, the absorption capacity of the roots to nutrition and moisture is poor, and the growth of the disease plants on the upper part of the ground is also influenced. Generally, the disease strain is not obvious in symptoms at the initial stage of damage, namely, the stem tip turns yellow, grows weak and is short, but gradually yellows and withers to die with the continuous increase of harm.
Allium tuberosum (Bradysia odoriphaga Yang et Zhang) is commonly called as Allium tuberosum maggot, belongs to Diptera, Eumycotina, Euonymus, and Allium, and mainly damages Allium tuberosum, Allium sativum, and other Liliaceae plants. For the characteristic of harm, adults lay eggs in soil gaps or soil blocks around the leek plants, after larvae are hatched, bulbs and tender stems gathered at the underground parts of the leeks are harmed, the young stems are rotted, the leek leaves are withered and yellow until rotten and dead, and the complete leek plant dies if severe.
Under the condition of double diseases and insect pests, the production of Chinese chives or other crops is seriously influenced, and the development of a pesticide which can resist diseases and kill insects is very significant, but a large amount of cost and time are needed for developing a new pesticide compound, and the cost and the time for developing the compound pesticide composition are relatively low, so the invention develops the compound pesticide composition for resisting diseases and killing insects.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a compound composition which has obvious synergy and can resist diseases and kill insects.
The invention also aims to provide application of the compound composition to agricultural pests, in particular to application to prevention or control of Chinese chive maggots, phyllotreta striolata, root knot nematode disease, blight, damping off, root rot, soft rot and the like.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the active ingredients of the compound composition comprise fluopyram and chlorfenapyr, wherein the weight ratio of the fluopyram to the chlorfenapyr is 1: 50-50: 1, preferably 1: 30-40: 1, more preferably 1: 10-12: 1, and most preferably 5:3, 2:3, 5:7, 4:3, 11:14 and 1: 4.
The active ingredients in the compound composition account for 5-60 percent of the composition by weight, preferably 12-50 percent of the composition by weight, and the balance is auxiliary agents and/or carriers allowed to be used in agriculture.
The compound composition can be prepared into any agriculturally acceptable formulation, and the preferred formulation comprises a microcapsule suspending agent, a soluble solution, a suspending agent, granules and a suspended seed coating agent.
The auxiliary agent and/or the carrier is selected from one or a mixture of more than two of solvent, emulsifier, dispersant, preservative, capsule wall material, binder, thickener, wetting agent, antifreeze agent, disintegrant, stabilizer, defoamer, slow release agent, film forming agent, colorant, filler and deionized water.
The solvent can be one or more of cyclohexanone, trimethylbenzene, ethanol, N-propanol, isopropanol, propylene glycol, glycerol, N-butanol, acetone, butanone, toluene, xylene, tetramethylbenzene, methylnaphthalene, methyl oleate, ethyl acetate, fatty acid methyl ester, pine oil, soybean oil, isooctanol, sec-butyl acetate, dimethyl carbonate, dibasic ester, N-methylpyrrolidone, N-dimethylacetamide, fatty acid methyl ester and pine resin-based vegetable oil.
The emulsifier can be one or a mixture of more than two of calcium alkyl benzene sulfonate, phenyl phenol polyoxyethylene ether phosphate, alkylphenol formaldehyde resin polyoxyethylene ether phosphate, phenyl phenol polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether, EO/PO block copolymer, fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, sorbitan monostearate and sorbitan fatty acid ester polyoxyethylene ether.
The dispersing agent can be one or a mixture of more than two of alkyl naphthalene sulfonate, naphthalene sulfonic acid formaldehyde condensate, polycarboxylate, lignosulfonate and fatty alcohol-polyoxyethylene ether.
The antiseptic can be one or more of formaldehyde, benzoic acid, sodium benzoate, potassium sorbate, Kathon.
The capsule wall material can be selected from polyurethane, poly toluene-2, 4-diisocyanate, polyvinyl acetate, cellulose phthalate, polyacrylate, polyamide, multifunctional aminoplast, and one or more than two of ethyl cellulose, hydroxyethyl cellulose, ethylenediamine and sodium polystyrene sulfonate.
The binder can be one or more of glucose, sucrose, hydroxyethyl cellulose, soluble starch, polyethylene glycol, methyl cellulose, polycarboxylic acids and water-soluble high molecular compound sodium carboxymethyl cellulose, polyvinyl alcohol, paraffin, gypsum, hydroxypropyl methyl cellulose and polyacrylamide.
The thickener is selected from one or more of xanthan gum, organic bentonite, magnesium aluminum silicate, white carbon black, gelatin, acacia and polyvinyl alcohol.
The wetting agent can be one or a mixture of more than two of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, fatty alcohol-polyoxyethylene ether, dioctyl sulfosuccinate, nekal BX, sodium lauryl sulfate, wetting penetrant F and saponin powder.
The antifreeze agent can be one or a mixture of more than two of ethylene glycol, propylene glycol, glycerol, polyethylene glycol, sorbitol, urea and inorganic salts.
The disintegrating agent can be one or more of sodium carbonate, bentonite, urea, ammonium sulfate, glucose, soluble starch and aluminum chloride.
The stabilizer can be one or more of sodium citrate, resorcinol, epoxidized soybean oil, epichlorohydrin, epoxidized linseed oil, pentaerythritol, xylitol and butyl hydroxy anisole.
The defoaming agent is one or the combination of more than two of silicon defoaming agent, polyether defoaming agent, higher alcohol, tributyl phosphate and polysiloxane.
The slow release agent can be selected from one or two of polyvinyl alcohol and Arabic gum.
The film forming agent can be one or a mixture of more than two of polyvinyl alcohol, polyvinylpyrrolidone, polyester resin, acrylic acid synthetic polymer, acrylic acid resin film forming agent, chitosan, butadiene resin film forming agent and polyurethane film forming agent.
The colorant is selected from one of basic rose bengal, permanent red and disperse blue.
The filler can be selected from one or more of kaolin, corn starch, ammonium sulfate, light calcium carbonate, white carbon black, kaolin, bentonite, light calcium carbonate, gypsum, attapulgite, urea, sucrose, diatomite, potassium dihydrogen phosphate, pottery clay and sepiolite.
The built compositions of the invention can also be applied in combination with other active ingredients, for example for widening the spectrum of control or preventing the development of resistance. Such as fungicides, bactericides, attractants, insecticides, acaricides, nematicides, growth regulators, herbicides, safeners, fertilizers, semiochemicals and the like.
The compound composition disclosed by the invention is applied to preventing or controlling agricultural pests.
Preferably, the agricultural pests are chive maggots and chive root-knot nematodes.
Compared with the prior art, the insecticidal composition has the beneficial effects that:
(1) the compound composition disclosed by the invention achieves the effect of treating insect pests and diseases by one-time application through the compounding of the fluopyram and the chlorfenapyr, is time-saving and labor-saving, and particularly has a good effect of preventing and treating chive maggot and chive root nematode disease.
(2) The compound composition has the effect of preventing and treating pests, is obviously improved compared with a single component, and well shows that the compound of the fluopyram and the chlorfenapyr has a synergistic effect.
(3) The compound composition disclosed by the invention has the advantages that the prevention and treatment effect is improved, the production cost is reduced, and the pollution to the environment is reduced.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more concise and clear, the present invention is described with the following specific embodiments, but the present invention is by no means limited to these embodiments. The following description is only a preferred embodiment of the present invention, and is only for the purpose of describing the present invention, and should not be construed as limiting the scope of the present invention. It should be understood that any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
First, preparation examples
Example 1: 48% Fluopyram-chlorfenapyr soluble solution (5:3)
Figure BDA0002065261200000051
The preparation method of the soluble agent comprises the following steps: the materials are mixed according to a proportion, added into a preparation kettle and fully mixed and stirred evenly, and then the 48 percent fluopyram-chlorfenapyr soluble solution is prepared.
Example 2: 12% Fluopyram-chlorfenapyr suspending agent (5:7)
Figure BDA0002065261200000052
The preparation method of the suspending agent comprises the following steps: the materials are mixed according to a proportion, and the 12 percent fluopyram-chlorfenapyr suspending agent can be prepared after the materials are uniformly dispersed by high-speed shearing and are sanded in a sand mill.
Example 3: 21% Fluopyram-chlorfenapyr water dispersible granule (4:3)
Figure BDA0002065261200000061
The preparation method of the water dispersible granule comprises the following steps: the materials are mixed according to a proportion, and then are subjected to jet milling, uniform mixing, kneading granulation, drying and screening to prepare the 21% fluopyram-chlorfenapyr water dispersible granule.
Example 4: 5% Fluopyram-chlorfenapyr microcapsule suspending agent (1:4)
Figure BDA0002065261200000062
The preparation method of the microcapsule suspending agent comprises the following steps: the active ingredients of the fluopyram and the chlorfenapyr are mixed with the wall material of the poly toluene-2, 4-diisocyanate to form an oil phase, the oil phase is sheared to form an emulsion prepolymer, and then other auxiliary agents are added to be uniformly mixed, so that the 5 percent fluopyram-chlorfenapyr microcapsule suspending agent can be prepared.
Example 5: 50% Fluopyram-chlorfenapyr granules (11:14)
Figure BDA0002065261200000063
The preparation method of the granules comprises the following steps: the components are uniformly mixed to prepare a soft material, and the prepared soft material is granulated and dried by an extrusion granulator to prepare the granular formulation of the fluopyram-chlorfenapyr with the concentration of 50 percent.
Example 6: 30% Fluopyram-chlorfenapyr suspension seed coating agent (2:3)
Figure BDA0002065261200000071
The preparation method of the suspension seed coating agent comprises the following steps: firstly, mixing all the auxiliary agents, uniformly mixing the auxiliary agents through high-speed shearing, adding the active ingredients of fluopyram and chlorfenapyr, and grinding the mixture in a ball mill for 2 to 3 hours until the particle fineness of the suspending agent is below 5 microns to obtain the 30 percent fluopyram-chlorfenapyr suspended seed coating agent.
Example 7: 60% Fluopyram-chlorfenapyr granules (1:1)
Figure BDA0002065261200000072
The preparation method of the granules comprises the following steps: the raw materials are uniformly mixed in a mixing tank, and the 60 percent fluopyram-chlorfenapyr granules are prepared through extrusion, granulation, drying and screening.
Example 8: 51% Fluopyram-chlorfenapyr granules (50:1)
Figure BDA0002065261200000073
Figure BDA0002065261200000081
The preparation method of the granules comprises the following steps: the components are uniformly mixed to prepare a soft material, and the prepared soft material is granulated and dried by an extrusion granulator to prepare 51 percent fluopyram-chlorfenapyr granules.
Example 9: 31% Fluopyram-chlorfenapyr suspension seed coating agent (1:30)
Figure BDA0002065261200000082
The preparation method of the suspension seed coating agent comprises the following steps: firstly, mixing all the auxiliary agents, uniformly mixing the auxiliary agents through high-speed shearing, adding the active ingredients of fluopyram and chlorfenapyr, and grinding the mixture in a ball mill for 2 to 3 hours until the particle fineness of the suspending agent is below 5 microns to obtain the 31 percent fluopyram-chlorfenapyr suspended seed coating agent.
Example 10: 22% Fluopyram-chlorfenapyr granules (1:10)
Figure BDA0002065261200000083
The preparation method of the granules comprises the following steps: the materials are mixed according to a proportion, and the levigated active ingredients are evenly coated on a carrier wetted by polyethylene glycol in a mixer to prepare the 22 percent fluopyram-chlorfenapyr granules.
Second, indoor biological activity determination
1. Indoor toxicity determination of fluopyram and chlorfenapyr combined preparation on leek maggots
Test subjects: chinese chive and leek maggot
The test method comprises the following steps: the stomach toxicity contact killing combined method is adopted, namely the Chinese chives and the Chinese chive maggots are respectively treated by the medicament. The method specifically comprises the following steps: the combined preparation of the invention was diluted with 0.1% Triton X-100 to the solutions of the gradients used, respectively, and was ready for use. Pouring molten 2.5% agar to a thickness of 2mm in a six-hole bioassay plate, and after cooling, spreading round filter paper slightly smaller than the diameter of the bioassay hole on the agar. Cleaning and air drying folium Allii tuberosi with uniform thicknessCutting the stem into 1.5cm pieces, soaking in pesticide solution from low concentration to high concentration for 15s, taking out, air drying, and placing on circular filter paper in the bioassay hole. Picking 3-year-old Chinese chive maggot larvae with a wet fine hairbrush in an insect-soaking net, completely soaking the insect-soaking net in the liquid medicine for 15s, taking out the insect-soaking net, placing the insect-soaking net on absorbent paper to remove excessive water, and transferring the larvae into a six-hole bioassay plate paved with filter paper and Chinese chive pseudostems subjected to liquid medicine soaking treatment. Pasting the raw test orifice of the raw test plate with rice paper. The bioassay plate is placed in a dark place under the conditions that the temperature is 23 +/-1 ℃ and the relative humidity is 60%, the drug treatment is repeated for 3 times at each concentration of 1, and 20 test insects are adopted for each repetition of 1. Using 3-year-old chive bradysia odoriphaga larvae treated by 0.1% of TritonX-100 distilled water as a control, effectively determining the mortality rate of less than 10%, observing and recording the death number of the 3-year-old chive bradysia odoriphaga larvae after 24h, and calculating the mortality rate by touching the larvae with a writing brush and determining the death rate of the larvae with the bodies still. Referring to the pesticide test technology and evaluation method compiled by the Huang Yang Ministry, an equivalent comparison method is adopted: LC to target when single agent50Value, LC to target with synergist added to same agent50The value is determined if LC with synergist is added to the medicament50The value is smaller than that of the single medicament, and the synergistic effect is shown. The synergy is expressed by a synergy index:
Figure BDA0002065261200000091
generally, a synergy index greater than 1 indicates synergy, less than 1 is antagonistic, and a value equal to 1 is additive. The test results are shown in Table 1.
TABLE 1 indoor toxicity determination of combined preparations of fluopyram and chlorfenapyr against Chinese chive maggot
Reagent for testing Proportioning LC50(μg/ml) Index of synergy
Fluopyram --- --- ---
Chlorfenapyr --- 6.03 ---
Fluopyram: chlorfenapyr 5:3 5.69 1.06
Fluopyram: chlorfenapyr 5:7 4.82 1.25
Fluopyram: chlorfenapyr 4:3 5.64 1.07
Fluopyram: chlorfenapyr 1:4 5.29 1.14
Fluopyram: chlorfenapyr 11:14 5.53 1.09
Fluopyram: chlorfenapyr 2:3 5.48 1.10
Fluopyram: chlorfenapyr 1:1 5.58 1.08
Fluopyram: chlorfenapyr 50:1 5.97 1.01
Fluopyram: chlorfenapyr 1:30 5.43 1.11
Fluopyram: chlorfenapyr 1:10 5.34 1.13
Fluopyram: chlorfenapyr 1:50 5.20 1.16
Fluopyram: chlorfenapyr 40:1 5.85 1.03
Fluopyram: chlorfenapyr 12:1 5.74 1.05
As can be seen from Table 1, the synergistic index of the combination of fluopyram and chlorfenapyr in the range of 1: 50-50: 1 to chive and chive maggot is greater than 1, which indicates that the combination preparation of fluopyram and chlorfenapyr has synergistic effect.
2. Indoor toxicity determination of fluopyram and chlorfenapyr combined preparation on leek root knot nematode disease
Test subjects: root knot nematode disease of Chinese chives
The test method comprises the following steps: according to a biological measurement standard method NY/T1833.1-2009, an insect soaking method is adopted. Preparing a nematode suspension: picking up egg masses of root-knot nematodes from diseased leek stems, cleaning with sterile water, incubating at 20-25 deg.C to obtain uniform second-instar larvae, and adjusting the concentration of nematode suspension to about 300 per ml with sterile water for use. 3mL of liquid medicine is sequentially sucked from low concentration to high concentration by a liquid transfer device and respectively added into the test tube, then 3mL of prepared equivalent nematode suspension liquid is sucked and added into the test tube, and the liquid medicine of the fluopyram and the chlorfenapyr is respectively and uniformly mixed with the nematode suspension liquid in an equivalent way. Using a pipette to remove 2mL of the mixed solution in the Xiaosun of the Polygrandchild biochemical test plate, covering the plate, and culturing at the constant temperature of 25 ℃ for 24 h. Each treatment was repeated 4 times, and a treatment containing no drug solution was used as a control.
Performing statistical analysis by DPS statistical analysis software by calculating EC of each agent50The insecticidal activity of each drug was evaluated, and the co-toxicity coefficient (CTC value) of the mixture was calculated according to the grandsinope method.
Measured virulence index (ATI) ═ b (Standard reagent EC50Reagent for test EC50)×100。
Theoretical virulence index (TTI) ═ a agent virulence index x percent of a in the mixture + B agent virulence index x percent of B in the mixture. Co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].
According to the insecticidal combination division standard of NY/T11547.7-2006: the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80 < co-toxicity coefficient (CTC) < 120 showed additive effects.
TABLE 2 indoor toxicity determination of Fluopyram and Chlorfenapyr combination formulations for leek root knot nematode disease
Reagent for testing Proportioning EC50(μg/ml) Co-toxicity coefficient
Fluopyram --- 3.35 ---
Chlorfenapyr --- 15.28 ---
Fluopyram: chlorfenapyr 5:3 3.10 152.80
Fluopyram: chlorfenapyr 5:7 4.26 144.41
Fluopyram: chlorfenapyr 4:3 3.03 166.16
Fluopyram: chlorfenapyr 1:4 5.49 162.55
Fluopyram: chlorfenapyr 11:14 4.67 127.47
Fluopyram: chlorfenapyr 2:3 4.01 157.17
Fluopyram: chlorfenapyr 1:1 3.63 151.38
Fluopyram: chlorfenapyr 50:1 2.78 122.38
Fluopyram: chlorfenapyr 1:30 10.52 130.28
Fluopyram: chlorfenapyr 1:10 9.09 126.99
Fluopyram: chlorfenapyr 1:50 11.71 121.97
Fluopyram: chlorfenapyr 40:1 2.25 151.78
Fluopyram: chlorfenapyr 12:1 2.65 134.49
From the indoor toxicity test results in the table 2, it can be seen that the co-toxicity coefficient of fluopyram and chlorfenapyr in the range of 1: 50-50: 1 to the root knot nematode disease of Chinese chive is greater than 120, which indicates a synergistic effect, wherein the synergistic effect is most remarkable when the ratio of fluopyram to chlorfenapyr to the root knot nematode disease is 4:3, and the co-toxicity coefficient is 166.16.
Third, field test
1. Field efficacy test of combined preparation of fluopyram and chlorfenapyr
Test subjects: chinese chive maggot and Chinese chive root knot nematode
Reagent to be tested: the treatment agents were the combined preparations of examples 1-4 above, the control agents were 41.7% flupyraclostrobin suspension and 10% chlorfenapyr suspension, and the blank control was sprayed with equal amount of clear water.
The test method comprises the following steps: putting the above doses into a medicine barrel of a sprayer respectively, adding 15L of water to prepare a liquid medicine, and spraying the liquid medicine one by using a spray head aiming at the root stubbles of the Chinese chives, wherein each hole is sprayed with 20-25 mL. And after the fixed-point spraying is finished, irrigating the test cell with big water immediately, wherein the water depth is 4-5 cm.
The investigation method comprises the following steps: according to GB/T17980.67-2004 were investigated and slightly modified. Before application, the population base number of each cell is investigated, and then the leeks are cut off for application. And 7 th and 21d after the application, respectively sampling 5 points in a Z shape to investigate the number of the chive maggots and the number of the plant of the root-knot nematode in each cell, wherein 2 adjacent holes are formed in each point, 15-25 plants are formed in each hole, and 10 holes are investigated in each cell. The control effect was calculated from the survey data and the test results are shown in table 3.
TABLE 3 field efficacy test of Fluopyram and Chlorfenapyr combination formulations
Figure BDA0002065261200000121
Figure BDA0002065261200000131
As can be seen from the results of the field efficacy test in Table 3, the combined preparation of fluopyram and chlorfenapyr can effectively prevent and control leek maggots and leek root knot nematode, achieves the effect of preventing and controlling various diseases by one-time application, has obviously improved prevention effect compared with a single component, prevents and controls more than 80% of leek root knot nematode, prevents and controls more than 65% of leek maggots, does not find that the tested medicament has phytotoxicity to crops during the test, and indicates that the compound composition of the invention is safe to crops.
In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims (1)

1. The application of the compound composition for resisting diseases and killing insects in preventing or controlling agricultural pests is characterized in that active ingredients of the composition comprise fluopyram and chlorfenapyr, and the weight ratio of the fluopyram to the chlorfenapyr is 5:3, 2:3, 5:7, 4:3, 11:14 and 1: 4; the agricultural pest is Chinese chive maggot.
CN201910422806.2A 2019-05-21 2019-05-21 Compound composition for resisting diseases and killing pests Active CN110115267B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910422806.2A CN110115267B (en) 2019-05-21 2019-05-21 Compound composition for resisting diseases and killing pests

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910422806.2A CN110115267B (en) 2019-05-21 2019-05-21 Compound composition for resisting diseases and killing pests

Publications (2)

Publication Number Publication Date
CN110115267A CN110115267A (en) 2019-08-13
CN110115267B true CN110115267B (en) 2021-07-06

Family

ID=67522849

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910422806.2A Active CN110115267B (en) 2019-05-21 2019-05-21 Compound composition for resisting diseases and killing pests

Country Status (1)

Country Link
CN (1) CN110115267B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115474606B (en) * 2022-08-26 2024-06-21 南京林业大学 Fluopicolide and chlorfenapyr compound synergistic insecticidal composition

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1385068A (en) * 2002-05-22 2002-12-18 江苏省农药研究所 Composite with pest-killing, mite-killing bacteria-killing activity
US8778835B2 (en) * 2006-07-06 2014-07-15 Bayer Cropscience Ag Pesticidal composition comprising a pyridylethylbenzamide derivative and an insecticide compound

Also Published As

Publication number Publication date
CN110115267A (en) 2019-08-13

Similar Documents

Publication Publication Date Title
CN105766925B (en) A kind of composition pesticide of the mite of pyrrole containing nitrile ester and its application
CN104770390A (en) Suspension seed coating agent
CN103081937A (en) Compound weeding composition of pretilachlor and pyrazosulfuron-ethyl and granules of composition
CN102960361A (en) Insecticide composition and application thereof
CN110115267B (en) Compound composition for resisting diseases and killing pests
CN105901012A (en) Suspended seed coating prepared from pyraclostrobin, penflufen and clothianidin
CN102805102B (en) Disinsection combination containing fosthiazate and ryanodine receptor inhibitor insecticide
CN102405914A (en) Synergistic mite killing composition containing cyflumetofen and clofentezine
CN103875701A (en) Insecticidal composition for preventing and treating soil insects
CN104982443B (en) A kind of Herbicidal combinations of Han oxaziclomefones and mefenacet
CN110115268B (en) Pesticide composition containing tolfenpyrad and fluopyram
CN108651504B (en) Pesticide composition containing abamectin, fluxapyroxad and thiamethoxam
CN109287651B (en) Bifenthrin-containing medicament composition
CN105532711B (en) A kind of Pesticidal combination and application thereof containing clothianidin and cyromazine
CN114097773A (en) Suspending agent capable of preventing and controlling pine wood nematode disease
CN105145635A (en) Composition used for preventing and treating American white moths by helicopter chemical application and preparation method thereof
CN109169688A (en) A kind of Pesticidal combination and application thereof containing thiocyclam and emamectin-benzoate
CN108651514A (en) A kind of composition pesticide containing fosthiazate and fluxapyroxad
CN108902151A (en) Application of the Pesticidal combination on prevention and treatment noctuid and thrips
CN113475508B (en) Weeding composition
CN102771512B (en) Insecticidal composition containing fosthiazate and pyridaben
CN108056105A (en) A kind of composition pesticide containing Flupyrazofos-containpesticide and dinotefuran
CN108353902A (en) A kind of Herbicidal composition for rice field
CN110946146B (en) Synergistic nematicidal composition containing trifloxystrobin and fosthiazate
CN105766989B (en) A kind of agricultural pesticide composition containing sulphur insect amide and cyfloxylate and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant