CN110075769B - Sol preparation process and device for making xeromyces dormancy breaking - Google Patents
Sol preparation process and device for making xeromyces dormancy breaking Download PDFInfo
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- CN110075769B CN110075769B CN201910439787.4A CN201910439787A CN110075769B CN 110075769 B CN110075769 B CN 110075769B CN 201910439787 A CN201910439787 A CN 201910439787A CN 110075769 B CN110075769 B CN 110075769B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/0004—Preparation of sols
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Abstract
The invention discloses a preparation process and a device of sol capable of making dormant thelephora ganbajun sporocarp release dormancy and enter a growth period, wherein the preparation process comprises the steps of leaching rosin after microorganism killing in absolute ethyl alcohol in advance to obtain a brownish yellow absolute ethyl alcohol rosin solution, mixing the obtained absolute ethyl alcohol rosin solution with sterile water, preparing sol by adopting a centrifugal spraying method, and adding calcium nitrate and xylose into the prepared sol to prepare the sol capable of making the thelephora ganbajun dormancy release; the preparation device comprises a liquid storage tank, an absolute ethanol storage tank and a Nobel steam generator, a sectional sterilization method is adopted, the air tightness is good, the collosol can promote the rhizopus to be dormant, a favorable foundation is provided for the scientific research and propagation promoting technology of the rhizopus, the propagules of the rhizopus are further expanded, the biological community of the forest land can be improved, and the restoration of the damaged forest land is promoted.
Description
Technical Field
The invention relates to the technical field of sol preparation, in particular to a sol preparation process and a sol preparation device for making thelephora ganbajun dormant.
Background
At present, the utilization of forestry resources in China is showing an increasing trend, people gradually deepen the understanding of wild fungi in forests, but always stay at a level depending on natural environment giving and giving away, and in numerous researches, people only can research the habitat of the wild fungi through basic science and research the heredity of the wild fungi through molecular biology; the method is characterized in that the Sparassis crispa is taken as a typical example, because the Sparassis crispa has a dormant period, the formation of the Sparassis crispa needs a long period, people only can use an inefficient method to cultivate the increased propagules of the Sparassis crispa, the breeding and cultivating efficiency of the Sparassis crispa is very low, at present, a method for promoting spore germination of the Sparassis crispa by artificially interfering the dormant period of the spore of the Sparassis crispa is not disclosed, and the utilization of the precious resource of the Sparassis crispa is greatly limited.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a sol preparation process and a sol preparation device for making the pasteurella go dormant, wherein the sol capable of breaking the dormancy period of the pasteurella is prepared by a solution replacement method, and the sol has the advantages of high stability, simple reaction and easy control of the preparation process.
In order to achieve the above object, the technical solution of the present invention is achieved as follows.
A process for preparing a sol for making thelephora hibernate, comprising the steps of:
the method comprises the following steps: the microorganisms in the natural rosin are killed by using 400K to 450K and 250Kpa to 255Kpa at high temperature and high pressure;
step two: rosin obtained in the step one is mixed according to the mass ratio of rosin: and (3) anhydrous ethanol is 120-180: leaching in advance to 900-1000 ℃ to obtain a brown-yellow absolute ethyl alcohol and rosin solution, and filtering to remove insoluble long-chain resin and pine needle weeds in the absolute ethyl alcohol and rosin solution;
step three: adding the absolute ethyl alcohol, the rosin solution and the sterile water obtained in the step two into a solvent replacement kettle according to the volume ratio of 1:4, and dispersing into the sterile water by adopting a centrifugal spraying method to prepare sol;
step four: adding sodium dihydrogen phosphate or sodium bicarbonate into the sol, and adjusting the pH value of the sol to 5.5-6.0;
step five: and (3) adding calcium nitrate accounting for 0.1-0.2% of the mass ratio of the calcium nitrate to the sol and xylose accounting for 0.3-0.5% of the mass ratio of the xylose to the sol into the sol obtained in the fourth step to prepare the sol which can enable dormant thelephora ganbajun sporocarp to be released from dormancy and enter a growth period.
The specific conditions of the centrifugal spraying method are as follows: when the peripheral speed of the atomizing centrifugal disc reaches 60m/s, the rosin ethanol solution and the sterile water are slowly conveyed to the atomizing centrifugal disc, so that the rosin ethanol solution and the sterile water are atomized into spherical small droplets and then are fully mixed.
The technical scheme of the specific device for completing the preparation process is as follows:
a collosol preparation device for making thelephora ganbajun dormant comprises a liquid storage tank 2, an absolute ethyl alcohol storage tank 4 and a Nobel steam generator 27, wherein the liquid output end of the liquid storage tank 2 is connected with one end of a first liquid conveying pipe g-l1, a clear water pump 3 and a valve j25 are arranged in a pipeline of the first liquid conveying pipe g-l1, the other end of the first liquid conveying pipe g-l1 is connected with the liquid input end of a high-level liquid storage tank 7, and the liquid output end of the high-level liquid storage tank 7 is connected with the liquid input end of a first metering pump 8; the liquid output end of the absolute ethyl alcohol storage tank 4 is connected with one end of a second liquid conveying pipe g-l2, an oil pump 5 and a valve j24 are arranged in a pipeline of a second liquid conveying pipe g-l2, the other end of a second liquid conveying pipe g-l2 is connected with the absolute ethyl alcohol input end of a high-position absolute ethyl alcohol storage tank 6, the absolute ethyl alcohol output end of the high-position absolute ethyl alcohol storage tank 6 is connected with the absolute ethyl alcohol input end of a second metering pump 9, the liquid output end of the first metering pump 8 and the absolute ethyl alcohol output end of the second metering pump 9 are respectively connected with the liquid input end of the solvent replacement kettle 12, the liquid output end of the solvent replacement kettle 12 is connected with the liquid input end of the floating coil heat exchanger 19 through a diaphragm pump 18, and the liquid output end of the floating coil heat exchanger 19 is connected with the liquid input end of the component blending tank 20 through a constant pressure valve j20 and a bottom valve j 21; the air pressure input end of the allocation tank 20 is connected with the air pressure output end of the pressure-resistant steel cylinder 22 through a constant-pressure pipe, the air pressure output end of the allocation tank 20 is connected with the air pressure input end of the floating coil heat exchanger 19 through a constant-pressure pipe and a constant-pressure valve j20, the air pressure output end of the floating coil heat exchanger 19 is connected with the air pressure input end of the solvent replacement kettle 12, the air pressure output end of the solvent replacement kettle 12 is connected with the air pressure input end of the high-level liquid storage tank 7 through a first metering pump 8, the air pressure output end of the high-level liquid storage tank 7 is connected with the air pressure input end of the liquid storage tank 2 through a constant-pressure pipe, the other air pressure output end of the replacement kettle 12 is connected with the air pressure input end of the high-level liquid storage tank 7 through a second metering pump 9, and the air pressure output end of the high-level liquid storage tank 7 is connected with the air pressure input end of the anhydrous ethanol storage tank 4 through a constant-pressure pipe; the air pressure input end of the pressure-resistant steel cylinder 22 is connected with the air pressure output end of the vacuum buffer tank 23 through an air conveying pipe provided with a valve j16, the air pressure input end of the vacuum buffer tank 23 is connected with the vacuum pump 24, and the vacuum pump 24 is provided with a pressure gauge 25;
a steam output end of the nobes steam generator 27 is connected with a steam input end of the air distribution cylinder 26 through a sterilizing steam pipeline g1, a steam output end of the air distribution cylinder 26 is adjusted in flow direction through an adjusting valve j11 and a j12, the adjusting valve j11 is connected with the floating coil heat exchanger 19 through a sterilizing steam pipeline g3, the adjusting valve j12 is connected with a sterilizing steam pipeline g2, a first three-way joint ST1, a second three-way joint ST2 and a gas transmission valve j1 are arranged on a pipeline of the sterilizing steam pipeline g2, the sterilizing steam pipeline g2 is divided into two steam pipeline branches after passing through the gas transmission valve j1, the first branch is connected with the steam input end of the anhydrous ethanol storage tank 4, the second branch is connected with the steam input end of the anhydrous ethanol storage tank 4, the first branch forms a steam branch flow control system through valves j1, j2 and j3, and the second branch forms another steam return pipe 5 and j6 control system through valves j 4; the steam output end of the high-level liquid storage tank 7 is connected with a fourth three-way joint ST4 through a bent pipe and is connected with a sterilization steam pipeline g4, the steam input end of the absolute ethyl alcohol storage tank 4 is input from a fifth three-way joint ST5, passes through the absolute ethyl alcohol storage tank 4 and the high-level absolute ethyl alcohol storage tank 6 and is connected with a sterilization steam pipeline g4 through a sixth three-way joint ST6 through a bent pipe; the first three-way joint ST1 is connected with the steam input end of the solvent replacement kettle 12 and the floating disc heat exchanger 19 through valves j7, j8 and j9, respectively, the steam output end of the solvent replacement kettle 12 is connected with the steam input end of the floating disc heat exchanger 19 through a diaphragm pump 18, a valve j23 and a valve j22, and is branched through the floating disc heat exchanger 19, one steam output end of the floating disc heat exchanger 19 is connected with the steam input end of the component preparation tank 20 through a valve j21, the other steam output end of the floating disc heat exchanger 19 is connected with the sterilizing steam pipeline g4 through a valve j17, the steam output end of the component preparation tank 20 is connected with the steam input end of the pressure-resistant steel cylinder 22 through a valve j19, the steam output end of the pressure-resistant steel cylinder 22 is connected with the sterilizing steam pipeline g4 through a valve j10, the sterilizing steam pipeline g4 is converged into a sterilizing steam pipeline g5 at a ninth three-way joint ST9, and flows back into the Nobel steam generator 27 to be reheated, a closed loop is formed.
The solvent replacement kettle 12 is vertically arranged, a second motor 11 is arranged at the upper part of the solvent replacement kettle and drives a central shaft 10 of the replacement kettle through a second belt P2, the central shaft 10 is provided with a centrifugal atomizing disc W after being sealed through a sealing sleeve 15-1, a liquid conveying pipe penetrates through the solvent replacement kettle body 12 and is introduced into the centrifugal atomizing disc W through a tail end thin pipe, a liquid stirring rake 30 is arranged at the lower part of the solvent replacement kettle 12 and is sealed through the central shaft sealing sleeve 15 and connected with a first motor 16 through a first belt P1, and the first motor 16 is connected with a foundation through a motor foot support.
The floating coil heat exchanger 19 is connected with a double-pipe heat exchanger 21, and the double-pipe heat exchanger 21 is connected with a vacuum pump 24 for pressure reduction through a pressure-resistant steel cylinder 22 and a vacuum buffer tank 23.
The pipeline connecting the liquid storage tank 2 and the high-level liquid storage tank 7 is a shared pipe of a constant pressure pipe and a steam delivery pipe, the pipeline connecting the absolute ethyl alcohol tank 4 and the high-level liquid storage tank 6 is a shared pipe of a constant pressure pipe and a steam delivery pipe, and the pipeline g-l3 connecting the solvent replacement kettle 12 and the floating coil heat exchanger 19 is a shared pipe of a constant pressure pipe and a steam delivery pipe.
The gas transmission pipe g2, the gas transmission pipe g4 and the gas transmission pipe g5 are preferably gas transmission pipes with the diameter of 28 mm; the air conveying pipe g1 and the air conveying pipe g3 are preferably 35mm in diameter.
The component blending tank 20 is connected with a pressure-resistant steel cylinder 22 by a pipeline.
The nobes steam generator 27 is provided with valves j14 and j15 for regulating the flow of condensed water and steam respectively, and the steam flow needs to be wrapped by a pipeline for heat preservation treatment.
According to the sol preparation process and device for enabling thelephora ganbajun to be dormant, the prepared sol can be used for removing the dormancy of thelephora ganbajun, and a favorable foundation is provided for scientific research and propagation promoting technologies of thelephora ganbajun; the sol prepared by the method can further expand propagules of thelephora ganbajun zang, and is used for promoting propagation technology and improving forest biological communities; the invention can improve the growth condition of pine trees and promote the restoration of damaged forest lands by matching with the powder of the sparassis crispa sporocarp for rainy season application.
Drawings
FIG. 1 is a schematic view of a sol preparation apparatus for making Thelephora hibernating according to the present invention, wherein: FIG. 1(a) is a general view of a sol preparation apparatus for making Thelephora dormant; FIG. 1(b) is a piping diagram of a sol preparation apparatus for making thelephora dormant; FIG. 1(c) is a valve identification diagram of a sol preparation apparatus for making Thelephora dormant; FIG. 1(d) is a steam cycle diagram of a sol device for rendering the Thelephora dormant.
In the figure: 1. the device comprises a long-neck funnel, a 2-liquid storage tank, a 3-clean water pump, a 4-absolute ethanol storage tank, a 5-oil pump, a 6-high absolute ethanol storage tank, a 7-high liquid storage tank, a 8-first metering pump, a 9-second metering pump, a 10-central shaft of a replacement kettle, a 11-second motor, a 12-solvent kettle, a 13-liquid stirring rake, a 14-central shaft, a 15-shaft sleeve, a 16-first motor, a 17-motor foot support, a 18-diaphragm pump, a 19-floating coil heat exchanger, a 20-component allocation tank, a 21-sleeve heat exchanger, a 22-pressure-resistant steel bottle, a 23-vacuum buffer tank, a 24-vacuum pump, a 25-pressure gauge, a 26-gas distribution cylinder, a 27-Nobes steam generator, a W-centrifugal atomizing disk, a g-l1. first liquid conveying pipe, a g-l2. second liquid conveying pipe, a g-l3. constant-pressure pipe, g1, g2, g3, g4 and g5 which are sterilization steam pipelines, and j1, j2, j3, j4, j5, j6, j7, j8, j9, j10, j11, j12, j13, j14, j15, j16, j17, j17-b and j19 are gas transmission valves, j20, j21, j22, j23, j24 and j25 are gas transmission valves, ST1 is a first three-way joint, ST2 is a second three-way joint, ST3 is a third three-way joint, ST4 is a fourth three-way joint, ST5 is a fifth three-way joint, ST6 is a sixth three-way joint, ST7 is a seventh three-way joint, ST8 is an eighth three-way joint and ST9 is a ninth three-way joint.
FIG. 2 is a schematic diagram of the experimental effect of the sol preparation process for making Thelephora hibernating of the present invention, wherein: FIG. 2(a) shows the use of the finished sol in the spore germination test of Thelephora ganbajun; FIG. 2(b) is a soil inoculation experiment of Thelephora collocalifornica spores; FIG. 2(c) is a diagram of the development of a bacterial pond under natural conditions; FIG. 2(d) is a soil inoculation map of a spore germinant of Thelephora ganbajun; FIG. 2(e) is a sol effect diagram.
Detailed Description
The basic flow and the working principle of a sol preparation process and a sol preparation device for making thelephora ganbajun dormant are further described in detail with reference to the attached drawings.
Referring to fig. 1, a collosol preparation device for making thelephora ganbajun dormant comprises a liquid storage tank 2, an absolute ethyl alcohol storage tank 4 and a Nobel steam generator 27, wherein a liquid output end of the liquid storage tank 2 is connected with one end of a first liquid conveying pipe g-l1, a clear water pump 3 and a valve j25 are arranged in a pipeline of the first liquid conveying pipe g-l1, the other end of the first liquid conveying pipe g-l1 is connected with a liquid input end of a high-level liquid storage tank 7, and a liquid output end of the high-level liquid storage tank 7 is connected with a liquid input end of a first metering pump 8; the liquid output end of the absolute ethyl alcohol storage tank 4 is connected with one end of a second liquid conveying pipe g-l2, an oil pump 5 and a valve j24 are arranged in a pipeline of a second liquid conveying pipe g-l2, the other end of a second liquid conveying pipe g-l2 is connected with the absolute ethyl alcohol input end of a high-position absolute ethyl alcohol storage tank 6, the absolute ethyl alcohol output end of the high-position absolute ethyl alcohol storage tank 6 is connected with the absolute ethyl alcohol input end of a second metering pump 9, the liquid output end of the first metering pump 8 and the absolute ethyl alcohol output end of the second metering pump 9 are respectively connected with the liquid input end of the solvent replacement kettle 12, the liquid output end of the solvent replacement kettle 12 is connected with the liquid input end of the floating coil heat exchanger 19 through a diaphragm pump 18, and the liquid output end of the floating coil heat exchanger 19 is connected with the liquid input end of the component blending tank 20 through a constant pressure valve j20 and a bottom valve j 21; the air pressure input end of the allocation tank 20 is connected with the air pressure output end of the pressure-resistant steel cylinder 22 through a constant-pressure pipe, the air pressure output end of the allocation tank 20 is connected with the air pressure input end of the floating coil heat exchanger 19 through a constant-pressure pipe and a constant-pressure valve j20, the air pressure output end of the floating coil heat exchanger 19 is connected with the air pressure input end of the replacement kettle 12, the air pressure output end of the replacement kettle 12 is connected with the air pressure input end of the high-level liquid storage tank 7 through a first metering pump 8, the air pressure output end of the high-level liquid storage tank 7 is connected with the air pressure input end of the liquid storage tank 2 through a constant-pressure pipe, the other air pressure output end of the replacement kettle 12 is connected with the air pressure input end of the high-level liquid storage tank 7 through a second metering pump 9, and the air pressure output end of the high-level liquid storage tank 7 is connected with the air pressure input end of the anhydrous ethanol storage tank 4 through a constant-pressure pipe; the air pressure input end of the pressure-resistant steel cylinder 22 is connected with the air pressure output end of the vacuum buffer tank 23 through an air conveying pipe provided with a valve j16, the air pressure input end of the vacuum buffer tank 23 is connected with the vacuum pump 24, and the vacuum pump 24 is provided with a pressure gauge 25;
a steam output end of the nobes steam generator 27 is connected with a steam input end of the air distribution cylinder 26 through a sterilizing steam pipeline g1, a steam output end of the air distribution cylinder 26 is adjusted in flow direction through an adjusting valve j11 and a j12, the adjusting valve j11 is connected with the floating coil heat exchanger 19 through a sterilizing steam pipeline g3, the adjusting valve j12 is connected with a sterilizing steam pipeline g2, a first three-way joint ST1, a second three-way joint ST2 and a gas transmission valve j1 are arranged on a pipeline of the sterilizing steam pipeline g2, the sterilizing steam pipeline g2 is divided into two steam pipeline branches after passing through the gas transmission valve j1, the first branch is connected with the steam input end of the anhydrous ethanol storage tank 4, the second branch is connected with the steam input end of the anhydrous ethanol storage tank 4, the first branch forms a steam branch flow control system through valves j1, j2 and j3, and the second branch forms another steam return pipe 5 and j6 control system through valves j 4; the steam output end of the high-level liquid storage tank 7 is connected with a fourth three-way joint ST4 through a bent pipe and is connected with a sterilization steam pipeline g4, the steam input end of the absolute ethyl alcohol storage tank 4 is input from a fifth three-way joint ST5, passes through the absolute ethyl alcohol storage tank 4 and the high-level absolute ethyl alcohol storage tank 6 and is connected with a sterilization steam pipeline g4 through a sixth three-way joint ST6 through a bent pipe; the first three-way joint ST1 is connected with the steam input end of the solvent replacement kettle 12 and the floating disc heat exchanger 19 through valves j7, j8 and j9, respectively, the steam output end of the solvent replacement kettle 12 is connected with the steam input end of the floating disc heat exchanger 19 through a diaphragm pump 18, a valve j23 and a valve j22, and is branched through the floating disc heat exchanger 19, one steam output end of the floating disc heat exchanger 19 is connected with the steam input end of the component preparation tank 20 through a valve j21, the other steam output end of the floating disc heat exchanger 19 is connected with the sterilizing steam pipeline g4 through a valve j17, the steam output end of the component preparation tank 20 is connected with the steam input end of the pressure-resistant steel cylinder 22 through a valve j19, the steam output end of the pressure-resistant steel cylinder 22 is connected with the sterilizing steam pipeline g4 through a valve j10, the sterilizing steam pipeline g4 is converged into a sterilizing steam pipeline g5 at a ninth three-way joint ST9, and flows back into the Nobel steam generator 27 to be reheated, a closed loop is formed.
The solvent replacement kettle 12 is vertically arranged, a second motor 11 is arranged at the upper part of the solvent replacement kettle and drives a central shaft 10 of the replacement kettle through a second belt P2, the central shaft 10 is provided with a centrifugal atomizing disc W after being sealed through a sealing sleeve 15-1, a liquid conveying pipe penetrates through the solvent replacement kettle body 12 and is introduced into the centrifugal atomizing disc W through a tail end thin pipe, a liquid stirring rake 30 is arranged at the lower part of the solvent replacement kettle 12 and is sealed through the central shaft sealing sleeve 15 and connected with a first motor 16 through a first belt P1, and the first motor 16 is connected with a foundation through a motor foot support.
The floating coil heat exchanger 19 is connected with a double-pipe heat exchanger 21, and the double-pipe heat exchanger 21 is connected with a vacuum pump 24 for pressure reduction through a pressure-resistant steel cylinder 22 and a vacuum buffer tank 23.
The pipeline connecting the liquid storage tank 2 and the high-level liquid storage tank 7 is a shared pipe of a constant pressure pipe and a steam delivery pipe, the pipeline connecting the absolute ethyl alcohol tank 4 and the high-level liquid storage tank 6 is a shared pipe of a constant pressure pipe and a steam delivery pipe, and the pipeline g-l3 connecting the solvent replacement kettle 12 and the floating coil heat exchanger 19 is a shared pipe of a constant pressure pipe and a steam delivery pipe.
The gas transmission pipes g2, g4 and g5, the gas transmission valves j1, j2, j3, j4, j5, j6, j7, j8, j9, j17-b, j18, j19 and j10 form a steam pipeline system, and preferably the gas transmission pipes with the diameter of 28 mm; g1 and g3 are main steam conveying pipelines, and preferably air conveying pipes with the diameter of 35 mm.
The component blending tank 20 is connected with a pressure-resistant steel cylinder 22 by a pipeline.
The nobes steam generator 27 is provided with valves j14 and j15 for regulating the flow of condensed water and steam respectively, and the steam flow needs to be wrapped by a pipeline for heat preservation treatment.
The working principle of the device is as follows: saturated steam generated by the Nobes steam generator 27 1 is conveyed to the branch cylinder 26 through a gas conveying pipeline, the gas flow is regulated through regulating valves j11 and j12, so that the steam flows in a sterilizing steam pipeline g2 for heating sterilization, after the sterilization is finished, valves j17-b, a constant-pressure valve j20 and a vacuum pump pipeline valve j16 on the floating coil heat exchanger 19 are closed, sterilizing steam pipeline valves j1, j3, j4, j5, j8, j12, j10, j11, j14, j15, j17-b, j19, j21, j20 and j22 are opened, valves j2, j5, j7, j9, j18, j16, j13, j23, j24 and j25 are closed, the steam flows into the device through an air inlet pipeline of the sterilizing steam pipeline 2 and then flows out of a steam return pipe to be circulated to the Nobes steam generator 27 for secondary heating, and then is circulated; after the equipment is sterilized for 3 hours, closing valves j1, j3, j4, j5, j8, j12, j10, j11, j14, j15, j17-b, j19, j21, j20 and j22, opening valves j2, j5, j7, j9, j18, j16, j13, j23, j24 and j25, enabling the device to be in an external isolated working state, opening a vacuum pump 24, opening a gas conveying pipe valve j16, pumping gas in the device, enabling the device to be in a low-pressure environment, and reducing the boiling point of a mixed system of ethanol and water; adding sterile water into a liquid storage tank 2, opening a water delivery pump 3, delivering the sterile water to a high-level tank 7, adding an ethanol rosin solution taken out of a stainless steel barrel 1 into an anhydrous ethanol storage tank 4, opening an oil pump 5, delivering the ethanol rosin solution to a high-level anhydrous ethanol storage tank 6, slowly delivering the rosin ethanol solution to an atomizing centrifugal disc W by a second metering pump 9, simultaneously opening a first metering pump 8 to slowly deliver the sterile water to the atomizing centrifugal disc W, opening a second motor 11 to drive the atomizing centrifugal disc W to rotate and atomize the sterile water and the ethanol rosin solution, closing all delivery pumps when 25L of the rosin ethanol solution flows in, continuously driving a liquid stirring rake 13 by the motor to continuously mix sol, opening a liquid delivery pipe and an air delivery shared pipe between a solvent replacement kettle 12 and a reduced pressure distiller after 2min, completing solvent replacement of the sterile water and the anhydrous ethanol resin solution in the solvent replacement kettle, opening a pipeline j23 at the bottom of the solvent replacement kettle, closing j22, opening a bottom valve j21, opening a diaphragm pump 18, conveying the sol into a floating disc heat exchanger, decompressing and extracting ethanol, opening a bottom valve j21 of the floating disc heat exchanger after extracting the ethanol, allowing the sol to flow into a component blending tank 20 under the action of gravity after opening a constant pressure valve j20, and taking the sol out of the component blending tank 20 to be stored in a tank for later use; after the outflow, the valves j20 and j21 are closed, the vacuum pump is connected with the vacuum buffer tank through a pipeline, the valves j10 and j16 are closed during the operation, the vacuumizing operation is started, and the valve j16 is slowly opened. Opening a valve j17 after the reduced pressure distillation is finished, and recovering ethanol; taking out the prepared sol, adding sodium dihydrogen phosphate or sodium bicarbonate into the sol, and adjusting the pH value of the sol to be 5.5-6.0; and (3) adding calcium nitrate accounting for 0.1-0.2% of the mass ratio of the calcium nitrate to the sol and xylose accounting for 0.3-0.5% of the mass ratio of the xylose to the sol into the sol obtained in the fourth step to prepare the sol which can enable dormant thelephora ganbajun sporocarp to be released from dormancy and enter a growth period.
Example 1
The method comprises the following steps: the microorganisms in the natural rosin are killed by using 400k, 250kpa of high temperature and high pressure; sterilizing the preparation device, introducing steam, sealing and sterilizing for three hours, performing sectional sterilization in the sterilization process, closing each outlet valve, adjusting the steam flow direction by using a three-way air-saving valve to form loop sterilization, opening an adjusting valve on a pipeline of the diaphragm pump 18, simultaneously opening an air path valve of the component blending tank 20, and closing the air path valve after sterilization;
step two: rosin according to the mass ratio: anhydrous ethanol 120: 900, leaching in advance to obtain a brown yellow absolute ethyl alcohol rosin solution, and filtering to remove insoluble long-chain resin and pine needle weeds in the absolute ethyl alcohol rosin solution;
step three: opening a constant pressure pipeline valve j25 of the liquid conveying equipment, opening the clean water pump 3, adding 45L of sterile water into the liquid storage tank 2, adding the absolute ethyl alcohol rosin solution obtained in the step two into the absolute ethyl alcohol storage tank 4, then opening the oil pump 5, conveying the absolute ethyl alcohol rosin solution to the high-position absolute ethyl alcohol storage tank 6, slowly conveying the absolute ethyl alcohol rosin solution to the atomizing centrifugal disc 11 by the second metering pump 9, meanwhile, sterile water is turned on and is input into the first metering pump 8, the motor 16 is turned on to drive the atomizing centrifugal disc 11 to rotate to atomize the sterile water and the absolute ethyl alcohol rosin solution, after 10L of rosin ethanol solution flows in, all the delivery pumps are closed, the motor 16 continues to drive the liquid stirring rake 13 to continue mixing the sol, the diaphragm pump 18 below the solvent replacement kettle 12 is opened after 2min, the mixed sol enters a floating coil heat exchanger 19, and a valve j21 is opened, so that the sol flows into a low-level component preparation tank 20 by gravity;
step four: adding sodium dihydrogen phosphate or sodium bicarbonate into the sol, and adjusting the pH value of the sol to be 5.5;
step five: 110mg of sol is measured and placed in a component blending tank 20, and 110.44mg of sol capable of making thejonebacteria dormant is prepared after 0.11mg of calcium nitrate and 0.33mg of xylose are added through a pipeline connected to a blending tank 20.
Example 2
The method comprises the following steps: the high temperature and high pressure of 410k, 251kpa are used for killing microorganisms in the natural rosin; sterilizing the preparation device, introducing steam, sealing and sterilizing for three hours, performing sectional sterilization in the sterilization process, closing each outlet valve, adjusting the steam flow direction by using a three-way air-saving valve to form loop sterilization, opening an adjusting valve on a pipeline of the diaphragm pump 18, simultaneously opening an air path valve of the component blending tank 20, and closing the air path valve after sterilization;
step two: rosin according to the mass ratio: absolute ethanol 135: 920, leaching in advance to obtain a brown yellow absolute ethyl alcohol rosin solution, and filtering to remove insoluble long-chain resin and pine needle weeds in the absolute ethyl alcohol rosin solution;
step three: opening a constant pressure pipeline valve j25 of the liquid conveying equipment, opening the clean water pump 3, adding 40L of sterile water into the liquid storage tank, adding the absolute ethyl alcohol rosin solution obtained in the step two into the absolute ethyl alcohol storage tank 4, then opening the oil pump 5, conveying the absolute ethyl alcohol rosin solution to the high-position absolute ethyl alcohol storage tank 6, slowly conveying the absolute ethyl alcohol rosin solution to the atomizing centrifugal disc 11 by the second metering pump 9, meanwhile, sterile water is turned on and is input into the first metering pump 8, the motor 16 is turned on to drive the atomizing centrifugal disc 11 to rotate to atomize the sterile water and the absolute ethyl alcohol rosin solution, after 9L of absolute ethyl alcohol rosin solution flows in, all the delivery pumps are closed, the motor 16 continues to drive the liquid stirring rake 13 to continue mixing the sol, the diaphragm pump 18 below the solvent replacement kettle 12 is opened after 2min, the mixed sol enters a floating coil heat exchanger 19, and a valve j21 is opened, so that the sol flows into a low-level component preparation tank 20 by gravity;
step four: adding sodium dihydrogen phosphate or sodium bicarbonate into the sol, and adjusting the pH value of the sol to be 5.6;
step five: 110mg of sol is measured and placed in a component blending tank 20, and 110.48mg of sol capable of making thejonebacteria dormant is prepared after 0.13mg of calcium nitrate and 0.35mg of xylose are added through a pipeline connected to a blending tank 20.
Example 3
The method comprises the following steps: using 425k, 252kpa of high temperature and high pressure to kill microorganisms in the natural rosin; sterilizing the preparation device, introducing steam, sealing and sterilizing for three hours, performing sectional sterilization in the sterilization process, closing each outlet valve, adjusting the steam flow direction by using a three-way air-saving valve to form loop sterilization, opening an adjusting valve on a pipeline of the diaphragm pump 18, simultaneously opening an air path valve of the component blending tank 20, and closing the air path valve after sterilization;
step two: rosin according to the mass ratio: absolute ethanol 144: 950 leaching in advance to obtain a brown yellow absolute ethyl alcohol rosin solution, and filtering to remove insoluble long-chain resin and pine needle weeds in the absolute ethyl alcohol rosin solution;
step three: opening a constant pressure pipeline valve j25 of the liquid conveying equipment, opening the clean water pump 3, adding 35L of sterile water into the liquid storage tank, adding the absolute ethyl alcohol rosin solution obtained in the step two into the absolute ethyl alcohol storage tank 4, then opening the oil pump 5, conveying the absolute ethyl alcohol rosin solution to the high-position absolute ethyl alcohol storage tank 6, slowly conveying the absolute ethyl alcohol rosin solution to the atomizing centrifugal disc 11 by the second metering pump 9, meanwhile, sterile water is turned on and is input into the first metering pump 8, the motor 16 is turned on to drive the atomizing centrifugal disc 11 to rotate to atomize the sterile water and the absolute ethyl alcohol rosin solution, after 8L of absolute ethyl alcohol rosin solution flows in, all the delivery pumps are closed, the motor 16 continues to drive the liquid stirring rake 13 to continue mixing the sol, the diaphragm pump 18 below the solvent replacement kettle 12 is opened after 2min, the mixed sol enters a floating coil heat exchanger 19, and a valve j21 is opened, so that the sol flows into a low-level component preparation tank 20 by gravity;
step four: adding sodium dihydrogen phosphate or sodium bicarbonate into the sol, and adjusting the pH value of the sol to be 5.7;
step five: 110mg of sol is measured and placed in a component blending tank 20, and after 0.15mg of calcium nitrate and 0.40mg of xylose are added through a pipeline connected to a blending tank 20, 110.55mg of sol capable of making thelephora ganbajun dormant is prepared.
Example 4
The method comprises the following steps: the high temperature and high pressure of 440k, 253kpa are used for killing microorganisms in the natural rosin; sterilizing the preparation device, introducing steam, sealing and sterilizing for three hours, performing sectional sterilization in the sterilization process, closing each outlet valve, adjusting the steam flow direction by using a three-way air-saving valve to form loop sterilization, opening an adjusting valve on a pipeline of the diaphragm pump 18, simultaneously opening an air path valve of the component blending tank 20, and closing the air path valve after sterilization;
step two: rosin according to the mass ratio: anhydrous ethanol ═ 156: 970 extracting in advance to obtain a brown yellow absolute ethyl alcohol rosin solution, and filtering to remove insoluble long-chain resin and pine needle weeds in the absolute ethyl alcohol rosin solution;
step three: opening a constant pressure pipeline valve j25 of the liquid conveying equipment, opening a clean water pump 3, adding 30L of sterile water into a liquid storage tank, adding the absolute ethyl alcohol rosin solution obtained in the step two into an absolute ethyl alcohol storage tank 4, then opening an oil pump 5, conveying the absolute ethyl alcohol rosin solution to a high-position absolute ethyl alcohol storage tank 6, slowly conveying the absolute ethyl alcohol rosin solution to an atomizing centrifugal disc 11 by a second metering pump 9, meanwhile, sterile water is turned on and is input into the first metering pump 8, the motor 16 is turned on to drive the atomizing centrifugal disc 11 to rotate to atomize the sterile water and the absolute ethyl alcohol rosin solution, after 7L of absolute ethyl alcohol rosin solution flows in, all the delivery pumps are closed, the motor 16 continues to drive the liquid stirring rake 13 to continue mixing the sol, the diaphragm pump 18 below the solvent replacement kettle 12 is opened after 2min, the mixed sol enters a floating coil heat exchanger 19, and a valve j21 is opened, so that the sol flows into a low-level component preparation tank 20 by gravity;
step four: adding sodium dihydrogen phosphate or sodium bicarbonate into the sol, and adjusting the pH value of the sol to be 5.8;
step five: 110mg of sol is measured and placed in a component blending tank 20, and 110.63mg of sol capable of making thejonebacteria dormant is prepared after 0.18mg of calcium nitrate and 0.45mg of xylose are added through a pipeline connected to a blending tank 20.
Example 5
The method comprises the following steps: the high temperature and high pressure of 450k, 255kpa are used for killing microorganisms in the natural rosin; sterilizing the preparation device, introducing steam, sealing and sterilizing for three hours, performing sectional sterilization in the sterilization process, closing each outlet valve, adjusting the steam flow direction by using a three-way air-saving valve to form loop sterilization, opening an adjusting valve on a pipeline of the diaphragm pump 18, simultaneously opening an air path valve of the component blending tank 20, and closing the air path valve after sterilization;
step two: rosin according to the mass ratio: absolute ethanol 180: 1000, leaching in advance to obtain a brown yellow absolute ethyl alcohol rosin solution, and filtering to remove insoluble long-chain resin and pine needle weeds in the absolute ethyl alcohol rosin solution;
step three: opening a constant pressure pipeline valve j25 of the liquid conveying equipment, opening the clean water pump 3, adding 25L of sterile water into the liquid storage tank, adding the absolute ethyl alcohol rosin solution obtained in the step two into the absolute ethyl alcohol storage tank 4, then opening the oil pump 5, conveying the absolute ethyl alcohol rosin solution to the high-position absolute ethyl alcohol storage tank 6, slowly conveying the absolute ethyl alcohol rosin solution to the atomizing centrifugal disc 11 by the second metering pump 9, meanwhile, sterile water is turned on and is input into the first metering pump 8, the motor 16 is turned on to drive the atomizing centrifugal disc 11 to rotate to atomize the sterile water and the absolute ethyl alcohol rosin solution, after 6L of absolute ethyl alcohol rosin solution flows in, all the delivery pumps are closed, the motor 16 continues to drive the liquid stirring rake 13 to continue mixing the sol, the diaphragm pump 18 below the solvent replacement kettle 12 is opened after 2min, the mixed sol enters a floating coil heat exchanger 19, and a valve j21 is opened, so that the sol flows into a low-level component preparation tank 20 by gravity;
step four: adding sodium dihydrogen phosphate or sodium bicarbonate into the sol, and adjusting the pH value of the sol to 6.0;
step five: 110mg of sol is measured and placed in a component blending tank 20, and 110.70mg of sol capable of making thejonebacteria dormant is prepared after 0.20mg of calcium nitrate and 0.50mg of xylose are added through a pipeline connected to a blending tank 20.
Claims (2)
1. A sol preparation process for making thelephora ganbajun dormant is characterized by comprising the following specific operation steps:
the method comprises the following steps: 400K-450K, 250 kPa-255 kPa high temperature and high pressure are used for killing microorganisms in the natural rosin;
step two: rosin obtained in the step one is mixed according to the mass ratio of rosin: and (3) anhydrous ethanol is 120-180: leaching in advance to 900-1000 ℃ to obtain a brown-yellow absolute ethyl alcohol and rosin solution, and filtering to remove insoluble long-chain resin and pine needle weeds in the absolute ethyl alcohol and rosin solution;
step three: adding the absolute ethyl alcohol, the rosin solution and the sterile water obtained in the step two into a solvent replacement kettle according to the volume ratio of 1:4, and dispersing into the sterile water by adopting a centrifugal spraying method to prepare sol;
step four: adding sodium dihydrogen phosphate or sodium bicarbonate into the sol, and adjusting the pH value of the sol to 5.5-6.0;
step five: and (3) adding calcium nitrate accounting for 0.1-0.2% of the mass ratio of the calcium nitrate to the sol and xylose accounting for 0.3-0.5% of the mass ratio of the xylose to the sol into the sol obtained in the fourth step to prepare the sol which can enable dormant thelephora ganbajun sporocarp to be released from dormancy and enter a growth period.
2. The process for preparing a sol for making pasteurella dormant according to claim 1, wherein said centrifugal spraying method is carried out under the specific conditions: when the peripheral speed of the atomizing centrifugal disc reaches 60m/s, the rosin ethanol solution and the sterile water are slowly conveyed to the atomizing centrifugal disc, so that the rosin ethanol solution and the sterile water are atomized into spherical small droplets and then are fully mixed.
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| CN2211817Y (en) * | 1995-03-01 | 1995-11-08 | 李春雁 | Pressure steam sterilizer |
| CN1382070A (en) * | 1999-10-21 | 2002-11-27 | 阿斯彭系统公司 | Rapid aerogel production process |
| KR20060131204A (en) * | 2005-06-15 | 2006-12-20 | 최의섭 | Silica sol automatic manufacture equipment |
| CN102939857A (en) * | 2012-11-13 | 2013-02-27 | 范宝福 | Ecological propagation method for wild sparassis crispa |
| CN206566887U (en) * | 2017-02-24 | 2017-10-20 | 益科博能源科技（上海）有限公司 | A kind of aeroge production system |
| CN109569448A (en) * | 2018-12-06 | 2019-04-05 | 西华大学 | Realize many varieties, self-interacting automatic integrated aerogel preparation facilities |
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|---|---|---|---|---|
| CN2211817Y (en) * | 1995-03-01 | 1995-11-08 | 李春雁 | Pressure steam sterilizer |
| CN1382070A (en) * | 1999-10-21 | 2002-11-27 | 阿斯彭系统公司 | Rapid aerogel production process |
| KR20060131204A (en) * | 2005-06-15 | 2006-12-20 | 최의섭 | Silica sol automatic manufacture equipment |
| CN102939857A (en) * | 2012-11-13 | 2013-02-27 | 范宝福 | Ecological propagation method for wild sparassis crispa |
| CN206566887U (en) * | 2017-02-24 | 2017-10-20 | 益科博能源科技（上海）有限公司 | A kind of aeroge production system |
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