CN109609571A - A kind of preparation method of anacidity chitosan oligosaccharide - Google Patents
A kind of preparation method of anacidity chitosan oligosaccharide Download PDFInfo
- Publication number
- CN109609571A CN109609571A CN201811573112.0A CN201811573112A CN109609571A CN 109609571 A CN109609571 A CN 109609571A CN 201811573112 A CN201811573112 A CN 201811573112A CN 109609571 A CN109609571 A CN 109609571A
- Authority
- CN
- China
- Prior art keywords
- chitosan oligosaccharide
- preparation
- anacidity
- chitosan
- anion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses a kind of preparation methods of anacidity chitosan oligosaccharide, comprising the following steps: chitosan is dissolved in hydrochloric acid solution, immobilised enzymes is added and is digested, ultrasound-assisted enzymolysis obtains enzymolysis liquid；Ultra filtration membrane, nanofiltration UF membrane are successively carried out to enzymolysis liquid, obtain chitosan oligosaccharide concentrate；Chitosan oligosaccharide concentrate passes through anion-exchange column；Spray drying, anacidity chitosan oligosaccharide is obtained, the present invention combines enzymatic isolation method with acid hydrolyzation, is aided with ultrasonic wave dissolution, effectively shorten reaction time of enzymatic isolation method, production cost is reduced, meanwhile, anion-exchange column makes in chitosan oligosaccharide without acid ion, that breaks traditional chitosan oligosaccharide applies bottleneck, it is had broad application prospects in food, medicine and cosmetic field, improves the yield of chitosan oligosaccharide, reduce environmental pollution.
Description
Technical field
The present invention relates to technical field of biochemical industry, in particular to a kind of preparation method of anacidity chitosan oligosaccharide.
Background technique
Chitosan oligosaccharide is called Chitosan poly oligosaccharide, chitosan oligomer, is that chitosan (is also had useization through special biological enzyme technology
Learn the report of degradation, microwave degradation technology) a kind of obtained degree of polymerization of degrading oligosaccharide product between 2 ~ 20, molecular weight≤
3200Da is water-soluble low molecular weight product preferable, function is big, bioactivity is high.It has chitosan unexistent
Higher solubility is dissolved in water entirely, is easy many unique functions such as to be absorbed and utilized by organism, it acts as the 14 of chitosan
Times.Meanwhile chitosan oligosaccharide is unique positively charged cationic basic amine group oligosaccharide in nature, is animal fiber element.
There is raising to be immunized, inhibit the growth of cancerous swelling cell, promote the formation of liver and spleen antibody, promotion calcium and minerals for chitosan oligosaccharide
Absorb, proliferation the human bodies such as Bifidobacterium and lactic acid bacteria profitable strain, reducing blood lipid, blood pressure lowering, it is hypoglycemic, adjust cholesterol, subtract
The functions such as fertilizer, prevention adult disease, can be applied to the fields such as medicine, functional food.It is negative that chitosan oligosaccharide can obviously eliminate human body oxygen
Ion radical activates body cell, delays senescence, and skin surface harmful bacteria is inhibited to breed, and performance of keeping humidity is excellent, is daily use chemicals neck
The basic material in domain.Chitosan oligosaccharide not only has water solubility, easy to use, and inhibits the spoilage organisms impact of performance significant, has both more
Kind function, is the wholefood antisepsis antistaling agent of function admirable.
The existing method for preparing chitosan oligosaccharide mainly has enzyme process and acid system, wherein enzyme process there are molecular weight low, reaction time
Length is unfavorable for the shortcomings that industrialized production, and acid system has acid residual and is difficult to remove, oligosaccharides purity bottom, is not suitable for answering in food
The shortcomings that using.
Summary of the invention
The present invention provides a kind of preparation methods of anacidity chitosan oligosaccharide.
To achieve the above object, the invention provides the following technical scheme:
A kind of preparation method of anacidity chitosan oligosaccharide, comprising the following steps:
S1: being dissolved in hydrochloric acid solution for chitosan, and immobilised enzymes is added and is digested, ultrasound-assisted enzymolysis obtains enzymolysis liquid；
S2: Ultra filtration membrane, nanofiltration UF membrane are successively carried out to enzymolysis liquid, obtains chitosan oligosaccharide concentrate；
S3: chitosan oligosaccharide concentrate passes through anion-exchange column, adsorbing chloride ions；
S4: spray drying obtains anacidity chitosan oligosaccharide.
Preferably, in the step S1, immobilised enzymes the preparation method comprises the following steps:
Entrapment media is reacted in liquid phase medium with coupling molecule, reaction temperature is 20-110 DEG C, reaction time 10-
20h, the dosage of coupling molecule are the 3%-30% of reaction system volume, and enzyme preparation is added, and reaction temperature is 20-25 DEG C, when reaction
Between be 20-25h, obtain immobilised enzymes.
Preferably, the entrapment media is aluminium oxide, bead, adsorbs resin or ion exchange resin, described to couple
Agent is aminopropyl triethoxysilane, epoxychloropropane or glutaraldehyde, and the liquid phase medium is organic phase or water phase.
Preferably, the enzyme preparation be one of chitosan enzyme, cellulase, bromelain or neutral proteinase or
The weight ratio of several combinations, the enzyme preparation and entrapment media is 0.5:1.
Preferably, in the step S1, the chitosan addition mass volume ratio is 5%-10%, the pH of the hydrochloric acid solution
Value 4.0-6.0, hydrolysis temperature are 35-45 DEG C, enzymolysis time 10-15h.
Preferably, the ultrasonic frequency is 25-70KHz.
Preferably, in the step S2, the molecular cut off of the ultrafiltration membrane is 1000-5000, the nanofiltration membrane aperture
For 1-4nm.
Preferably, in the step S3, the diameter of the anion-exchange column and length ratio are 1:6, and anion exchange
The anion exchange resin filled in column be 201 × 2,201 × 4,201 × 7,201 × 8,301,303 × 2,331, D201,
D202, D301, D302 or D311 one or more combination.
Preferably, the chitosan oligosaccharide concentrate flows through anion-exchange column with the flow velocity of 1.5-3.5BV/h.
Preferably, in the step S4, the inlet temperature of spray drying is 120-130 DEG C, outlet temperature 70-80
DEG C, spray pressure 0.5Mpa, the capacity of aspiration pump is 16-16.5m3/ h, the sample introduction speed of sampling pump are 4.5-6mL/
min。
The beneficial effects of the present invention are:
Enzymatic isolation method is combined with acid hydrolyzation, is aided with ultrasonic wave dissolution, effectively shortens reaction time of enzymatic isolation method, reduction is produced into
This, meanwhile, what anion-exchange column to break in chitosan oligosaccharide without acid ion traditional chitosan oligosaccharide applies bottleneck, food,
Medicine and cosmetic field have broad application prospects, and improve the yield of chitosan oligosaccharide, reduce environmental pollution.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment one:
A kind of preparation method of anacidity chitosan oligosaccharide, comprising the following steps:
S1: being dissolved in hydrochloric acid solution for chitosan, and immobilised enzymes is added and is digested, ultrasound-assisted enzymolysis obtains enzymolysis liquid, institute
Stating chitosan addition mass volume ratio is 5%, and the pH value 4.0 of the hydrochloric acid solution, hydrolysis temperature is 35 DEG C, and enzymolysis time is
10h, the ultrasonic frequency are 25KHz.
Wherein, immobilised enzymes the preparation method comprises the following steps: entrapment media is reacted in liquid phase medium with coupling molecule, instead
Answering temperature is 20 DEG C, reaction time 1h, and the dosage of coupling molecule is the 3% of reaction system volume, and enzyme preparation, reaction temperature is added
It is 20 DEG C, reaction time 20h obtains immobilised enzymes.The entrapment media be aluminium oxide, bead, absorption resin or from
Sub-exchange resin, the coupling molecule are aminopropyl triethoxysilane, epoxychloropropane or glutaraldehyde, and the liquid phase medium is to have
Machine phase or water phase.The enzyme preparation is one or more of chitosan enzyme, cellulase, bromelain or neutral proteinase
The weight ratio of combination, the enzyme preparation and entrapment media is 0.5:1.
S2: Ultra filtration membrane, nanofiltration UF membrane are successively carried out to enzymolysis liquid, obtains chitosan oligosaccharide concentrate, wherein described super
The molecular cut off of filter membrane is 1000, and the nanofiltration membrane aperture is 1nm.
S3: chitosan oligosaccharide concentrate passes through anion-exchange column, adsorbing chloride ions, the diameter and length of the anion-exchange column
Degree than be 1:6, and the anion exchange resin filled in anion-exchange column be 201 × 2,201 × 4,201 × 7,201 × 8,
301,303 × 2,331, D201, D202, D301, D302 or D311 one or more combination, the chitosan oligosaccharide concentrate is with 1.5-
The flow velocity of 3.5BV/h flows through anion-exchange column.
S4: spray drying obtains anacidity chitosan oligosaccharide, specifically, the inlet temperature of spray drying is 120 DEG C, outlet temperature
Degree is 70 DEG C, spray pressure 0.5Mpa, and the capacity of aspiration pump is 16m3/ h, the sample introduction speed of sampling pump are 4.5mL/min.
The pH value of anacidity chitosan oligosaccharide manufactured in the present embodiment is 7 or so, and product is faint yellow or yellow powder, molecular weight
≤ 3000, ash content≤1%, moisture≤8%, chloride ion content≤5%, solubility >=20%.
Embodiment two:
The part that the present embodiment is the same as example 1 repeats no more, unlike:
A kind of preparation method of anacidity chitosan oligosaccharide, comprising the following steps:
S1: being dissolved in hydrochloric acid solution for chitosan, and immobilised enzymes is added and is digested, ultrasound-assisted enzymolysis obtains enzymolysis liquid, institute
Stating chitosan addition mass volume ratio is 7%, and the pH value 5.0 of the hydrochloric acid solution, hydrolysis temperature is 40 DEG C, and enzymolysis time is
12h, the ultrasonic frequency are 50KHz.
Wherein, immobilised enzymes the preparation method comprises the following steps: entrapment media is reacted in liquid phase medium with coupling molecule, instead
Answering temperature is 65 DEG C, reaction time 15h, and the dosage of coupling molecule is the 17% of reaction system volume, and enzyme preparation, reaction temperature is added
It is 23 DEG C, the reaction time is for 24 hours, to obtain immobilised enzymes.The entrapment media be aluminium oxide, bead, absorption resin or from
Sub-exchange resin, the coupling molecule are aminopropyl triethoxysilane, epoxychloropropane or glutaraldehyde, and the liquid phase medium is to have
Machine phase or water phase.The enzyme preparation is one or more of chitosan enzyme, cellulase, bromelain or neutral proteinase
The weight ratio of combination, the enzyme preparation and entrapment media is 0.5:1.
S2: Ultra filtration membrane, nanofiltration UF membrane are successively carried out to enzymolysis liquid, obtains chitosan oligosaccharide concentrate, wherein described super
The molecular cut off of filter membrane is 4000, and the nanofiltration membrane aperture is 3nm.
S3: chitosan oligosaccharide concentrate passes through anion-exchange column, adsorbing chloride ions, the diameter and length of the anion-exchange column
Degree than be 1:6, and the anion exchange resin filled in anion-exchange column be 201 × 2,201 × 4,201 × 7,201 × 8,
301,303 × 2,331, D201, D202, D301, D302 or D311 one or more combination, the chitosan oligosaccharide concentrate with
The flow velocity of 2.5BV/h flows through anion-exchange column.
S4: spray drying obtains anacidity chitosan oligosaccharide, specifically, the inlet temperature of spray drying is 125 DEG C, outlet temperature
Degree is 75 DEG C, spray pressure 0.5Mpa, and the capacity of aspiration pump is 16.3m3/ h, the sample introduction speed of sampling pump are 5.5mL/
min。
Embodiment three:
The part that the present embodiment is the same as example 1 repeats no more, unlike:
A kind of preparation method of anacidity chitosan oligosaccharide, comprising the following steps:
S1: being dissolved in hydrochloric acid solution for chitosan, and immobilised enzymes is added and is digested, ultrasound-assisted enzymolysis obtains enzymolysis liquid, institute
Stating chitosan addition mass volume ratio is 10%, and the pH value 6.0 of the hydrochloric acid solution, hydrolysis temperature is 45 DEG C, and enzymolysis time is
15h, the ultrasonic frequency are 70KHz.
Wherein, immobilised enzymes the preparation method comprises the following steps: entrapment media is reacted in liquid phase medium with coupling molecule, instead
Answering temperature is 110 DEG C, reaction time 20h, and the dosage of coupling molecule is the 30% of reaction system volume, and enzyme preparation, reaction temperature is added
Degree is 25 DEG C, and reaction time 25h obtains immobilised enzymes.The entrapment media be aluminium oxide, bead, absorption resin or
Ion exchange resin, the coupling molecule are aminopropyl triethoxysilane, epoxychloropropane or glutaraldehyde, and the liquid phase medium is
Organic phase or water phase.The enzyme preparation is one of chitosan enzyme, cellulase, bromelain or neutral proteinase or several
The weight ratio of kind combination, the enzyme preparation and entrapment media is 0.5:1.
S2: Ultra filtration membrane, nanofiltration UF membrane are successively carried out to enzymolysis liquid, obtains chitosan oligosaccharide concentrate, wherein described super
The molecular cut off of filter membrane is 5000, and the nanofiltration membrane aperture is 4nm.
S3: chitosan oligosaccharide concentrate passes through anion-exchange column, adsorbing chloride ions, the diameter and length of the anion-exchange column
Degree than be 1:6, and the anion exchange resin filled in anion-exchange column be 201 × 2,201 × 4,201 × 7,201 × 8,
301,303 × 2,331, D201, D202, D301, D302 or D311 one or more combination, the chitosan oligosaccharide concentrate with
The flow velocity of 3.5BV/h flows through anion-exchange column.
S4: spray drying obtains anacidity chitosan oligosaccharide, specifically, the inlet temperature of spray drying is 130 DEG C, outlet temperature
Degree is 80 DEG C, spray pressure 0.5Mpa, and the capacity of aspiration pump is 16.5m3/ h, the sample introduction speed of sampling pump are 6mL/min.
The yield such as table 1 of anacidity chitosan oligosaccharide prepared by embodiment one to three:
| Embodiment one | Embodiment two | Embodiment three | |
| The yield (%) of chitosan oligosaccharide | 93 | 94 | 92 |
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment only includes one
A independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should will say
As a whole, the technical solutions in the various embodiments may also be suitably combined for bright book, and forming those skilled in the art can be with
The other embodiments of understanding.
Claims (10)
1. a kind of preparation method of anacidity chitosan oligosaccharide, which comprises the following steps:
S1: being dissolved in hydrochloric acid solution for chitosan, and immobilised enzymes is added and is digested, ultrasound-assisted enzymolysis obtains enzymolysis liquid；
S2: Ultra filtration membrane, nanofiltration UF membrane are successively carried out to enzymolysis liquid, obtains chitosan oligosaccharide concentrate；
S3: chitosan oligosaccharide concentrate passes through anion-exchange column；
S4: spray drying obtains anacidity chitosan oligosaccharide.
2. a kind of preparation method of anacidity chitosan oligosaccharide according to claim 1, which is characterized in that in the step S1, Gu
Surely change enzyme the preparation method comprises the following steps:
Entrapment media is reacted in liquid phase medium with coupling molecule, reaction temperature is 20-110 DEG C, reaction time 10-
20h, the dosage of coupling molecule are the 3%-30% of reaction system volume, and enzyme preparation is added, and reaction temperature is 20-25 DEG C, when reaction
Between be 20-25h, obtain immobilised enzymes.
3. a kind of preparation method of anacidity chitosan oligosaccharide according to claim 2, which is characterized in that the entrapment media is
Aluminium oxide, bead, absorption resin or ion exchange resin, the coupling molecule are aminopropyl triethoxysilane, epoxy chloropropionate
Alkane or glutaraldehyde, the liquid phase medium are organic phase or water phase.
4. a kind of preparation method of anacidity chitosan oligosaccharide according to claim 3, which is characterized in that the enzyme preparation is poly- for shell
The combination of one or more of carbohydrase, cellulase, bromelain or neutral proteinase, the enzyme preparation and entrapment media
Weight ratio be 0.5:1.
5. a kind of preparation method of anacidity chitosan oligosaccharide according to claim 2, which is characterized in that in the step S1, institute
Stating chitosan addition mass volume ratio is 5%-10%, and the pH value 4.0-6.0 of the hydrochloric acid solution, hydrolysis temperature is 35-45 DEG C, enzyme
The solution time is 10-15h.
6. a kind of preparation method of anacidity chitosan oligosaccharide according to claim 5, which is characterized in that the ultrasonic frequency is
25-70KHz。
7. a kind of preparation method of anacidity chitosan oligosaccharide according to claim 5, which is characterized in that in the step S2, institute
The molecular cut off for stating ultrafiltration membrane is 1000-5000, and the nanofiltration membrane aperture is 1-4nm.
8. a kind of preparation method of anacidity chitosan oligosaccharide according to claim 7, which is characterized in that in the step S3, institute
State anion-exchange column diameter and length ratio be 1:6, and the anion exchange resin filled in anion-exchange column be 201 ×
2,201 × 4,201 × 7,201 × 8,301,303 × 2,331, D201, D202, D301, D302 or D311 one or more group
It closes.
9. a kind of preparation method of anacidity chitosan oligosaccharide according to claim 8, which is characterized in that the chitosan oligosaccharide concentrate
Anion-exchange column is flowed through with the flow velocity of 1.5-3.5BV/h.
10. a kind of preparation method of anacidity chitosan oligosaccharide according to claim 9, which is characterized in that in the step S4, spray
The dry inlet temperature of mist is 120-130 DEG C, and outlet temperature is 70-80 DEG C, spray pressure 0.5Mpa, the exhaust of aspiration pump
Amount is 16-16.5m3/ h, the sample introduction speed of sampling pump are 4.5-6mL/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811573112.0A CN109609571A (en) | 2018-12-21 | 2018-12-21 | A kind of preparation method of anacidity chitosan oligosaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811573112.0A CN109609571A (en) | 2018-12-21 | 2018-12-21 | A kind of preparation method of anacidity chitosan oligosaccharide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109609571A true CN109609571A (en) | 2019-04-12 |
Family
ID=66010119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811573112.0A Pending CN109609571A (en) | 2018-12-21 | 2018-12-21 | A kind of preparation method of anacidity chitosan oligosaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109609571A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112980905A (en) * | 2021-03-11 | 2021-06-18 | 烟台泓源生物肥料有限公司 | Preparation method of chitosan oligosaccharide |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001017978A (en) * | 1999-07-07 | 2001-01-23 | Cosmo Shokuhin Kk | Production of partial hydrolyzate of organic matter |
| US20030109490A1 (en) * | 2001-07-09 | 2003-06-12 | You Hyung Ja | Composite stimulating iNOS enzyme which induce immuno-reactant nitric oxide synthesis and process for preparing the same |
| CN1772915A (en) * | 2004-11-10 | 2006-05-17 | 中国科学院大连化学物理研究所 | Chitosn producing process with immobilized enzyme |
| CN103360514A (en) * | 2013-06-24 | 2013-10-23 | 广西民族大学 | Method for preparing water-soluble chitosan oligosaccharide by quick degradation |
| CN103804515A (en) * | 2012-11-09 | 2014-05-21 | 扬州鸿信生物制品有限公司 | Chitooligosaccharide production method |
| CN106317255A (en) * | 2015-07-08 | 2017-01-11 | 惠州长龙生物技术有限公司 | Method for preparing neutral chitooligosaccharide by ion-exchange resin deacidification |
-
2018
- 2018-12-21 CN CN201811573112.0A patent/CN109609571A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001017978A (en) * | 1999-07-07 | 2001-01-23 | Cosmo Shokuhin Kk | Production of partial hydrolyzate of organic matter |
| US20030109490A1 (en) * | 2001-07-09 | 2003-06-12 | You Hyung Ja | Composite stimulating iNOS enzyme which induce immuno-reactant nitric oxide synthesis and process for preparing the same |
| CN1772915A (en) * | 2004-11-10 | 2006-05-17 | 中国科学院大连化学物理研究所 | Chitosn producing process with immobilized enzyme |
| CN103804515A (en) * | 2012-11-09 | 2014-05-21 | 扬州鸿信生物制品有限公司 | Chitooligosaccharide production method |
| CN103360514A (en) * | 2013-06-24 | 2013-10-23 | 广西民族大学 | Method for preparing water-soluble chitosan oligosaccharide by quick degradation |
| CN106317255A (en) * | 2015-07-08 | 2017-01-11 | 惠州长龙生物技术有限公司 | Method for preparing neutral chitooligosaccharide by ion-exchange resin deacidification |
Non-Patent Citations (4)
| Title |
|---|
| PETER ELIAS KIDIBULE等: "Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity", 《MICROBIAL CELL FACTORIES 》 * |
| 杨苏亮等: "壳寡糖的理化特性及制备方法", 《饲料博览》 * |
| 王振伟等: "超声波辅助酶法制备壳寡糖及抗氧化活性研究", 《中国食品添加剂》 * |
| 黄永春等: "超声作用下淀粉酶降解壳聚糖的研究", 《食品科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112980905A (en) * | 2021-03-11 | 2021-06-18 | 烟台泓源生物肥料有限公司 | Preparation method of chitosan oligosaccharide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| FI114807B (en) | Enzyme treatment of glucans | |
| CN100526335C (en) | Chitin oligose preparing process | |
| CN102492760B (en) | Method for producing active Rana dybowskii collagen oligopeptide by using compound enzyme | |
| CN1184237C (en) | Preparation of crust oligosaccharide and use | |
| Verma et al. | Enzyme immobilization on chitin and chitosan-based supports for biotechnological applications | |
| CN107226871B (en) | preparation method of highland barley β -glucan | |
| CN107868805B (en) | Longan polysaccharide degraded by lactobacillus fermentation and preparation method thereof | |
| CN110903488A (en) | Preparation method of chitosan @ metal organic framework antibacterial material | |
| CN107312807A (en) | The enzymolysis preparation of the brown alga function oligosaccharides in one main laminaria source | |
| CN110301601A (en) | A kind of soluble Kelp Powder and its Production by Enzymes method containing brown alga oligose | |
| CN107475226A (en) | A kind of new Bromenzyme and preparation method thereof | |
| CN1884308A (en) | Process for preparing chitosan oligosacharides | |
| CN109609571A (en) | A kind of preparation method of anacidity chitosan oligosaccharide | |
| CN107177586A (en) | A kind of bioconversion immobilization glusulase and preparation method thereof | |
| CN109880874A (en) | A kind of preparation method of rice active peptide, rice active peptide and its application | |
| CN106011206A (en) | Method for preparing bioactive peptide from immobilized bi-enzyme of composite carrier magnetic nano-particles | |
| CN107460178B (en) | Preparation method of mannase and extraction refining device thereof | |
| CN108997512A (en) | A kind of low molecular weight chitosan oligosaccharide and preparation method thereof | |
| CN108239177A (en) | A kind of method that purifying prepares high-quality biological polysaccharide | |
| CN1327852C (en) | A health food possessing immunity regulating function and method for preparing the same | |
| CN106281966B (en) | It is a kind of it is efficient production kappa-carrageenan oligosaccharide bioreactor and its application | |
| CN108484692A (en) | A method of the high efficiency extraction Glucosamine from zymotic fluid | |
| CN104531804A (en) | Method for production of crystal trehalose and co-production of moisturizing syrup | |
| CN103451233A (en) | Method for preparing theabrownin by taking tea polyphenol as active ingredients through adopting compound enzyme process | |
| CN105907819A (en) | Biological enzymolysis production method for controllable narrow molecular weight functional chitosan oligosaccharide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190412 |
|
| RJ01 | Rejection of invention patent application after publication |