CN110073909A - A kind of implantation methods improving campanulaceae yield and content of drug effect components - Google Patents
A kind of implantation methods improving campanulaceae yield and content of drug effect components Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a kind of implantation methods for improving campanulaceae yield and content of drug effect components, belong to Chinese medicine planting technology field, and the method for use is to carry out spraying DA-6 processing, and preferably spray DA-6 and combine processing with frustrating flower bud topping treatment；The present invention can increase campanulaceae single plant root fresh weight and 50% or more dry weight, and increase by 45% or more campanulaceae root diameter, while can substantially reduce lateral root number using spraying DA-6 and frustrating the method that flower bud topping treatment combines；56% or more total saponins of platycodon grandiflorum content in campanulaceae can also be increased, increase by 150% or more platycodin-D content in campanulaceae, the active constituent content in the yield and medicinal material of the especially middle river campanulaceae of campanulaceae is significantly improved, there is significant economic benefit.
Description
Technical field
The present invention relates to Chinese medicine planting technology field more particularly to a kind of campanulaceae yield and content of drug effect components of improving
Implantation methods.
Background technique
Campanulaceae (Platycodon grandiflorum (Jacq.) A.DC.), also known as Dao Laji, burden flower, are Campanulaceae
Platycodon grandiflouorum herbaceos perennial.Its root has important medical value, can promoting the dispersing function of the lung relieving sore-throat, eliminating the phlegm apocenosis, dampness elimination heresy, the smooth gas of tune
Machine, the promoting flow of qi and blood circulation, stop palpitate with fear tranquilizing mind；It is spent and bud has much ornamental value, and spire and root are also good vegetables, is collection medicine, food, reward
For all over the body.Modern pharmacology research points out that the main pharmacological component of campanulaceae is platycodin constituents, including campanulaceae soap
Glycosides-D, platycodin A, platycodin B, platycodin E etc.." Chinese Pharmacopoeia " is with the content of total saposins and platycodin-D height
Determine the quality of medicinal campanulaceae.
Campanulaceae is to be embodied in " Chinese Pharmacopoeia " to commonly use one of 40 taste Chinese medicines, and now the Chinese producing region of current campanulaceae medicinal material is mainly divided
Cloth is on Sichuan Zhong Jiang, Anhui Taihe county, Chifeng and other places.Through research study on the spot, at present only Inner Mongol campanulaceae large-scale plantation at
It is ripe, but Inner Mongol campanulaceae purposes is to make based on export food.Sichuan Zhong Jiang is also the Genuine producing area of campanulaceae, but campanulaceae cultivation is still protected
Conventional method plantation is held, is easy to be lost nutrient to aerial part, is unfavorable for reaching full growth for individual under ground portion, campanulaceae low output
Under.This makes domestic campanulaceae be used as medicine market, there is obvious supply falls short of demand relationship.Seek high yield, high-quality campanulaceae planting technology is mesh
Preceding urgent problem.
In order to improve the yield and content of drug effect components of campanulaceae, many effort have been done in this field.
The organ of plant is divided into nutrition organs and reproductive organs, according to plant growth rules: root system absorbs before florescence
Largely transport promotes vegetation growth of plant to root, stem, leaf nutrition organs to nutrient；Flowering fruit bearing stage nutrient is largely transported to reproduction
Organ promotes plant reproductive growth.It is root that middle river campanulaceae, which is used as medicine partially, i.e. the nutrition organs of plant.Therefore campanulaceae flowering fruit bearing stage
It is easy to be lost nutrient to bud part, is unfavorable for reaching full growth for root.
In recent years, have largely about frustrate flower bud topping (also known as Topping) to campanulaceae yield and product Quality Research.Such as by
Zhang little Bin is in campanulaceae Topping treatment research, showing that Topping method has certain inhibition to campanulaceae overground part reproductive growth
Effect, there is certain effect of increasing production under ground portion, is embodied in root long growth, root diameter thickening, fraction number are reduced；He Junjin
The influence that row defloration sequence, topping grows Shanglou campanulaceae is studied, it was demonstrated that at while Shanglou campanulaceae carries out defloration sequence, pinched
Reason effect is best, and plant vegetative growth can be promoted by being in particular in, inhibits reproductive growth, hence it is evident that promotes the growth of root, promotes
Effective component is accumulated to root；In the campanulaceae growth and development research of Zhang Yanling, also indicates that and annual campanulaceae progress squaring period is beaten
Top, can effectively increase yield, have certain castering action to total saponin content.But the middle river campanulaceae as genunie medicinal materials,
Growing environment, plant characteristic be equal to it is aforementioned significantly different, it is unknown to its effect to frustrate flower bud topping treatment.
Summary of the invention
The object of the invention is that a kind of implantation methods for improving campanulaceae yield and content of drug effect components are provided, to solve
The above problem.
To achieve the goals above, the technical solution adopted by the present invention is that such: a kind of raising campanulaceae yield and drug effect
The implantation methods of component content carry out spraying DA-6 processing in plantation.
The DA-6 of the application, i.e. Octacide 264, diethyl aminoethyl hexanoate, chemical name are that diethylammonium salt base ethyl alcohol ester, molecular formula are
C12H25NO2, it is a kind of novel plant growth accelerator, nonhazardous lipid material.It can promote the metabolism such as plant cell C, N and cell
Division, elongation growth.The research application of DA-6 is mentioned in lot of documents, DA-6 can promote plant leaf physiological change, improve
The photosynthetic rate of blade.In the research of wangdan etc., 10mgL is sprayed to the Blumea balsamifera in the growth retardation phase in winter-1
DA-6 can promote the increase of Blumea balsamifera growth and leaf dry weight, improve the content of nascent metabolism effective component；Dan Shouming, Liu Guo
Outstanding person's etc. research shows that can influence stomatal conductance, the Ye Lv of blade to the greenhouse peach progress 20mg/L DA-6 processing after the florescence
Cellulose content and chloroplaset Hill reaction vigor, non-cycle photophosphorylation activity, Rubiseo and the active size of ATPase,
Significantly improve the Net Photosynthetic Rate of peach blade.DA-6 can also promote the accumulation of fruits/plant biomass, Yan Cong etc. studies have shown that
After DA-6 is sprayed, ginger Later growth root, stem and leaf Fresh Yuxincao has obvious amplification, improves 22.30% yield of ginger；In Hu Zhaoping, Lee
It is big equal studies have shown that eggplant stem thickness, leaf area increase, and eggplant yield improves 36.9% after 0.01% DA-6 processing,
The content of the vitamin C of fruit, soluble sugar, crude fibre and crude protein increases in various degree simultaneously.In other correlative studys,
DA-6 processing can also promote the output increased of the plants such as strawberry, tomato, romaine lettuce, loquat, peanut.
These studies have shown that DA-6 processing fruits and vegetables class vine growth and development is had a significant impact, can promote plant leaf increasing
Greatly, it improves leaf photosynthesis efficiency and significantly improves the yield of rhizome, fruit to promote plant strain growth.
But as known to those skilled in the art, different plants are since its growing environment is different, growth characteristics are different, phase
Same medicament role is different, that is to say, that DA-6 not can promote all plants to grow and improve yield, right
It is even more there is unpredictability, and DA-6 handles the influence to medicinal material campanulaceae also not at present in the influence of chemical component in plant
It appears in the newspapers.Present inventor is by the way that experimental results demonstrate DA-6 river campanulaceae especially middle for campanulaceae has significant increase
Produce and improve the effect of root effective component.
As a preferred technical scheme: also carrying out frustrating flower bud topping treatment.
As a preferred technical scheme: the campanulaceae is middle river campanulaceae.Method of the invention, more suitable for middle river campanulaceae
The effect of plantation, volume increase and raising effective component for middle river campanulaceae is especially pronounced.
As further preferred technical solution: the middle river campanulaceae is biennial middle river campanulaceae.
As a preferred technical scheme: the method for spraying DA-6 processing is to spray in campanulaceae bud stage.
As a preferred technical scheme: uniformly being sprayed in Leaf of Platycodon Grandiflorum later for 5 points in the afternoon, the DA-6 concentration sprayed is
3-8mg/L, next day repeat spraying.
It is become apparent in middle river campanulaceae suitable growth period and suitable spraying time, effect.
As further preferred technical solution: the flower bud topping treatment of frustrating is carried out in campanulaceae bud stage.
As technical solution still more preferably: the method for frustrating flower bud topping treatment is to prescind every plant of tangerine using reaping hook
The top of stalk and bud.
Compared with the prior art, the advantages of the present invention are as follows: present invention use sprays DA-6 and mutually ties with flower bud topping treatment is frustrated
The method of conjunction can increase campanulaceae single plant root fresh weight and dry weight up to 50% or more, and increase campanulaceae root diameter up to 45% or more,
Lateral root number can be substantially reduced simultaneously；56% or more total saponins of platycodon grandiflorum content in campanulaceae can also be increased, increase campanulaceae in campanulaceae
150% or more saponin(e-D content significantly improves the active constituent content in the yield and medicinal material of the especially middle river campanulaceae of campanulaceae,
With significant economic benefit.
Detailed description of the invention
Fig. 1 is crop field sampling side ideograph；
Fig. 2 is that the HPLC figure of platycodin-D detection in the middle river campanulaceae of Different treatments [can only be adopted in patent accompanying drawing
With artwork master, so please using the map of different colours in Fig. 2 not, synteny mark is to distinguish, or with the side of arrow mark
Formula is good, if it is arrow mark, please use " 1 ", " 2 ", " 3 " such mark, and illustrate " 1 ", " 2 ", " 3 " in the literature respectively
What represents].
Specific embodiment
The present invention will be further described with reference to the accompanying drawings.
Embodiment 1
A kind of implantation methods improving campanulaceae yield and content of drug effect components, in plantation, in biennial campanulaceae bud stage
It carries out frustrating flower bud topping, the top and bud of every plant of campanulaceae is prescinded using reaping hook, obtain the middle river campanulaceae of " processing A ".
Embodiment 2
A kind of implantation methods improving campanulaceae yield and content of drug effect components carry out spraying DA-6 processing, tool in plantation
Body method are as follows: DA-6 is uniformly sprayed to biennial campanulaceae bud stage blade, after the specific time sprayed is selection at 5 points in afternoon,
With agricultural atomizer, taking 300ml concentration is 3 ‰ DA-6 reagents, and 15kg clear water is added and is diluted to 6mg/L, then uniform in blade
It sprays, next day repeat spraying, obtains the middle river campanulaceae of " processing B ".
Embodiment 3
A kind of implantation methods improving campanulaceae yield and content of drug effect components, in plantation, in biennial campanulaceae bud stage
It carries out frustrating flower bud topping, the top and bud of every plant of campanulaceae is prescinded using reaping hook, also, bud stage blade uniformly sprays DA-6,
The specific time sprayed is after selecting at 5 points in afternoon, and with agricultural atomizer, taking 300ml concentration is 3 ‰ DA-6 reagents, is added
15kg clear water is diluted to 6mg/L, then uniformly sprays in blade, next day repeat spraying, obtains the middle river campanulaceae of " processing C ".
Embodiment 4
Biennial middle river campanulaceae is conventionally planted, DA-6 is not sprayed, also without frustrating flower bud topping, is obtained
The middle river campanulaceae of " processing D ".
Effect experiment verifying:
1 test material
Campanulaceae chooses the Sichuan Zhongjiang County bird's nest village biennial campanulaceae close with the height on block ground, bud stage.
2 major experimental instruments and drug
2.1 instrument
Electric heating constant-temperature blowing drying box (DHG-9420)；High performance liquid chromatograph (Agilent HPLC)；Chromatographic column (XB-C18)；Ultrasonic cleaner (ULTRASONIC CLEANER)；Ultraviolet-visible spectrophotometer
(SHIMADZU UV-2450)；Thermostat water bath (XMTD-8222)；Pulverizer (JD-07)；Electronic analytical balance (SHIMADZU
AUY220)；Electronic vernier caliper.
2.2 reagent
Platycodin-D for Reference Substance (mass fraction >=98%) is purchased from Chengdu Man Site Biotechnology Co., Ltd；85% second
Alcohol；3 ‰ DA-6 solution；5% vanillic aldehyde-glacial acetic acid (ready-to-use)；Water saturated n-butanol；Perchloric acid；Glacial acetic acid.
3 experimental field overviews
It is experimental field set to Sichuan Deyang Zhong Jiang campanulaceae standardized planting base, belongs to Zhongjiang County bird's nest village, is located at Chuanzhong Qiuling
Area.Geographical coordinate is between 104 ° 26 ' -105 ° 15 ' of east longitude, 30 ° 31 ' -31 ° 17 ' of north latitude.Mean sea level is on the left side 500-600m
It is right.Soil property is sandy loam, and soil fertility is uniform.Campanulaceae cultivated ginseng is operated according to Sichuan Zhong Jiang campanulaceae standardized planting technical standard
Regulation, seed drilling, sowing quantity are 1.5-2.0Kg/ mus, apply farmyard manure.Make ridge in width 1.2-1.5m, high 15-20cm, trench digging
In 30-40cm wide.After seedling grows, in time to the weeding of campanulaceae crop field, fertilizing management.
4 test campanulaceae processing
Biennial 0.5 mu of campanulaceae planting site of Zhongjiang County is chosen, crop field shape is rectangle.Arrangement of sample plot at 12 sample prescriptions,
Each quadrat size is 0.5m2.Lateral separation 2m between each sample prescription, longitudinal gap 1m.It carries out as described in previous embodiment 1-4
Four groups of processing (number A, B, C, D), every group of processing (number A in triplicate1,A2,A3、B1,B2,B3、C1,C2,C3、D1,D2,
D3), sample prescription serial number is randomly ordered using Excel 2010, as shown in figure institute 1；
The usually weeding, watering management of middle river campanulaceae are consistent with other campanulaceaes plantation field management during entire test.?
Campanulaceae harvest time carries out sample acquisition, measures the root economical character and active constituent content of campanulaceae.
4.1 test campanulaceae morphological index measurements
The campanulaceae of number is successively carried out to fresh weight weighing, dry weight weighing, root measurement, root diameter measurement, and records data.
Fresh weight measurement: in the campanulaceae autumn harvest maturity period, take sample prescription campanulaceae, clear water is cleaned three times, with electronic analytical balance pair
Every plant of campanulaceae more measures.Every plant of number marks and records data, and unit g retains two-decimal.
Dry weight measurement: the campanulaceae root of number is placed in electric heating constant-temperature blowing drying box, 60 DEG C of constant temperature dryings are surveyed per hour
Corresponding dry weight is obtained, what difference was not more than 0.05g between weighing value twice is constant weight and records.
Root diameter measurement: with the main root maximum gauge for the campanulaceae that electronics vernier caliper measurement is numbered, unit mm, and recording
In corresponding table.
Lateral root number: for record number campanulaceae in addition to main root, the number of fleshy root, unit is item.
Root long: it using the extreme length of tape measuring number campanulaceae main root pulp, unit cm, and is recorded in corresponding
In table.
The measurement of 4.2 total saponin contents
This experiment uses EtOH Sonicate extraction method, prepares the total saposins sample liquid of campanulaceae to be measured.Vanilla root rot is used again
For color developing agent, Platycodin D is the colorimetric method of reference substance[20-24]Carry out the measurement of total saponin content.To compare quality c
It (mg) is abscissa, absorbance A is ordinate, carries out regression analysis using software Excel 2010, obtains regression equation are as follows: A
=0.0727c-0.0287, R2=0.9824, linear relationship is good.It calculates and records data.
The measurement of 4.3 platycodin-Ds
With high performance liquid chromatography, each group Platycodon Root of number is placed in Chengdu Pu Sisheng referring to Chinese Pharmacopoeia method
Object Science and Technology Ltd. carries out platycodin-D assay.Reference substance uses platycodin-D (the general think of biotechnology stock in Chengdu
Part Co., Ltd, lot number: 010184), to test sample, sample introduction is analyzed, and record chromatogram is as shown in Figure 2.
5. data processing
Data are handled using Excel 2010, dry weight, fresh weight, root diameter, lateral root number, root long is calculated and increases production ratio；Calculate total soap
Glycosides linear regression curves and content, platycodin-D analysis are compared.SPSS21.0 analyzes data, examines with one-way analysis of variance
Test the significance of difference between result data.
6. experimental result
Influence of 6.1 different disposals to campanulaceae root biomass
According to table 1, Different treatments influence campanulaceae root biomass different:
Compared with control treatment group (D group), individually uses and frustrate flower bud topping (A group), spray DA-6 (B group) and apply simultaneously
The increase of campanulaceae root biomass, and significant difference between each processing can be obviously promoted with DA-6 (C group) is sprayed by frustrating flower bud topping.Its
In apply simultaneously and frustrate flower bud topping and compared with other processing significantly increase campanulaceae single plant root fresh weight, dry weight with DA-6 (C group) processing is sprayed,
Amplification respectively reaches 42.02%, 45.87%.Illustrate to frustrate flower bud topping and is frustrated better than exclusive use with spraying DA-6 and be used in mixed way effect
Flower bud topping individually sprays DA-6 processing, and two kinds of processing modes, which combine, there is significant mutually collaboration to increase middle river campanulaceae yield
The effect of effect.
The variation of 1 different disposal campanulaceae root biomass of table
| Processing | Single plant root fresh weight/g plants-1 | Single plant root dry weight/g plants-1 |
| A | 14.81±0.88bc | 4.98±0.39b |
| B | 16.50±0.98b | 5.21±0.53b |
| C | 18.86±1.20a | 6.01±0.47a |
| D(CK) | 13.28±0.76c | 4.12±0.35c |
Note: different letters indicate significant difference (P < 0.05) in same column.Similarly hereinafter.
Note: license is considered for technical effect than heavier, so, in order to improve authorization probability, it is proposed that above-mentioned
Mark red data to be adjusted, be preferably formed with effect as " 1+1 > 2 ", adjust standard are as follows: the value of C group be adjusted so as to it is larger,
D class value is adjusted so as to smaller.Subsequent table 2/3/4 also suggests adjusting in this way
Influence of 6.2 different disposals to campanulaceae root diameter, root long and lateral root number
According to table 2, compared with control treatment group (D group), biennial campanulaceae bud stage Different treatments can be significant
Promote campanulaceae root diameter to increase, reduce lateral root item number, root long is influenced not significant.Frustrating flower bud topping treatment (A group) can increase single plant
Campanulaceae root diameter 1.52mm, spraying DA-6 processing (B group) can increase single plant campanulaceae root diameter 2.28mm；And it frustrates flower bud topping and sprays
DA-6 mixed processing (C group) increases the significant effect of campanulaceae root diameter, reaches 3.77mm.Simultaneously on reducing lateral root number,
It frustrates flower bud topping and sprays DA-6 mixed processing (C group) and independent DA-6 (B group) effect that sprays is preferable, lateral root number mean value is no more than 2
Item.In each group processing kind, the influence to the growth of campanulaceae root long is not significant.Illustrate that individually to can increase campanulaceae root straight using flower bud topping is frustrated
Diameter reduces lateral root number, but simultaneously using frustrating flower bud topping and to spray DA-6 mixed processing effect best, two kinds of processing modes in conjunction with pair
Yu Zhongjiang campanulaceae increases campanulaceae root diameter, reduction lateral root number has the function of significant mutually synergy；Individually spray DA-6
The effect of processing is taken second place.
2 different disposal campanulaceae root diameter of table, root long and the variation of lateral root number
| Processing | Root diameter/mm | Root long/cm | Lateral root number |
| A | 10.97±0.53b | 17.50±0.67a | 2.07±0.13b |
| B | 11.73±0.50b | 17.00±0.74a | 1.95±0.12c |
| C | 13.22±0.59a | 18.48±0.63a | 1.78±0.12c |
| D(CK) | 9.45±0.13c | 17.82±0.59a | 3.35±0.15a |
The influence of 6.3 Different treatments total saponins of platycodon grandiflorum contents
As shown in Table 3, compared with the control group, the total saposins that several test process can improve campanulaceae root to a certain degree contain
Amount.Flower bud topping is wherein frustrated with bud stage and sprays that DA-6 effect is best, and total saposins reach 9.12%, soap more total than control group (D group)
Glycosides content increases by 47.81%.It individually sprays DA-6 (C group) effect to take second place, improves single plant campanulaceae radical content 19.77%；Individually frustrate
Flower bud topping (A group) increases total saponin content 16.88%.Illustrate to frustrate biennial middle river campanulaceae bud stage flower bud topping and sprays DA-6
Total saponins of platycodon grandiflorum content can be substantially improved in mixed processing, and two kinds of processing modes, which are combined, increases total saponins of platycodon grandiflorum for middle river campanulaceae
Content has the function of significant mutually synergy, promotes accumulation of the campanulaceae medicinal ingredient to root, promotes the quality of campanulaceae.
The variation of 3 Different treatments total saponins of platycodon grandiflorum content of table
| Processing | A | B | C | D(CK) |
| Total saponin content | 7.21% | 7.39% | 9.12% | 6.17% |
Influence of 6.4 Different treatments to platycodin-D content
As shown in Table 4, in four kinds of processing modes, the content of platycodin-D is above 2015 editions Chinese Pharmacopoeias in campanulaceae root
Defined campanulaceae root is used as medicine content standard (>=0.10%).Campanulaceae bud stage is individually carried out frustrating flower bud topping treatment (A group), individually be applied
With DA-6 (B group), frustrates flower bud topping and spray mixed processing (C group) with DA-6, platycodin-D content is followed successively by 0.23%,
0.27%, 0.37%.Wherein mixed processing (C group) promotion platycodin-D content increase effect is sprayed to frustrate flower bud topping with DA-6
It preferably, is 2.31 times of control group (D group) content, significant effect.Illustrate to carry out frustrating flower bud topping, spray to biennial campanulaceae bud stage
DA-6 is applied, campanulaceae medicinal ingredient can be effectively improved, promote the quality of campanulaceae, and frustrates flower bud topping and sprays mixed processing with DA-6
For effect better than being administered alone, two kinds of processing modes, which combine, has significantly mutually middle river campanulaceae increase platycodin-D content
Synergistic effect.
The variation of 4 Different treatments platycodin-D content of table
| Processing | A | B | C | D(CK) |
| Platycodin-D content | 0.23% | 0.27% | 0.37% | 0.16% |
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (8)
1. a kind of implantation methods for improving campanulaceae yield and content of drug effect components, it is characterised in that: in plantation, sprayed
DA-6 processing.
2. a kind of implantation methods for improving campanulaceae yield and content of drug effect components according to claim 1, it is characterised in that:
It also carries out frustrating flower bud topping treatment.
3. a kind of implantation methods for improving campanulaceae yield and content of drug effect components according to claim 1, it is characterised in that:
The campanulaceae is middle river campanulaceae.
4. a kind of implantation methods for improving campanulaceae yield and content of drug effect components according to claim 3, it is characterised in that:
The middle river campanulaceae is biennial middle river campanulaceae.
5. a kind of implantation methods for improving campanulaceae yield and content of drug effect components according to claim 1, it is characterised in that:
The method for spraying DA-6 processing is to spray in campanulaceae bud stage.
6. a kind of implantation methods for improving campanulaceae yield and content of drug effect components according to claim 5, it is characterised in that:
It is uniformly sprayed in Leaf of Platycodon Grandiflorum later for 5 points in the afternoon, the DA-6 concentration sprayed is 3-8mg/L, next day repeat spraying.
7. a kind of implantation methods for improving campanulaceae yield and content of drug effect components according to claim 2, it is characterised in that:
The flower bud topping treatment of frustrating is carried out in campanulaceae bud stage.
8. a kind of implantation methods for improving campanulaceae yield and content of drug effect components according to claim 7, it is characterised in that:
The method for frustrating flower bud topping treatment is top and the bud that every plant of campanulaceae is prescinded using reaping hook.
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| CN104488478A (en) * | 2014-11-06 | 2015-04-08 | 普定县吉磊科技种植有限责任公司 | Highly-efficient cultivation method for Platycodon grandiflorum |
| CN104855116A (en) * | 2015-06-02 | 2015-08-26 | 山东省农业科学院作物研究所 | Cultivation method for improving yield of sweet potatoes in hilly and mountainous areas of northern potato planting region |
| CN107046968A (en) * | 2016-12-19 | 2017-08-18 | 安徽潜山县美林生态农业开发有限公司 | A kind of implantation methods of high yield balloonflower root |
| CN106613711A (en) * | 2017-01-26 | 2017-05-10 | 新疆农垦科学院 | Cultivation method capable of improving commodity rate and consistency of mature jujube orchard fruits |
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