CN1100587A - Method for prepn. of multi-element edible fungus protein - Google Patents
Method for prepn. of multi-element edible fungus protein Download PDFInfo
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Abstract
The preparation method involves the teps of : 1. domesticating hedgehog hydnum fungus and golden mushroom fungus in culture medium with Se, Ge, Zn additives by concentration gradient pressure method, and domesticating mushroom in culture medium with Se, Ge additives: 2. Cultivating hedgehog hydnum and golden mushroom in culture stuff with Se, Ge, Zn additives, and cultivating mushroom in culture stuff with Se, Ge additive; 3. drying hedgehog hydnum, golden muhroom and mushroom, and grinding them into powder, mixing them according to the proportion of 1:2:2, the product is a nutriment rich in Se, Ge and Zn.
Description
The present invention relates to a kind of preparation method of multielement edible fungus protein body.
At present, production of edible mushroom and application study mainly contain four aspects: (1) cultivates all kinds of edible fruit body of edible fungi in a large number; (2) deep processing of fruit body of edible fungi, as make dried, salt marsh, cylinder root of Dahurian angelica etc.; (3) liquid fermentation of edible mushroom and processed and applied; (4) the new edible mushroom kind of seed selection.These contents all are confined to the intrinsic characteristics of a certain edible mushroom itself are studied and used, and to how changing or the directed research that improves some effective ingredient in the edible mushroom is very few.
The objective of the invention is to provide a kind of preparation method of multielement edible fungus protein body, its goods are not only nutritious, and have been rich in trace elements such as selenium, germanium, zinc, have to promote function of human body, the remarkable efficacy of building up health.
Main design of the present invention is:
One, selects following edible fungus species for use
(A) Eumycota Basidiomycetes Aphyllophorales Hericium erinaceus material
The Hericium Hericium erinaceus
Formal name used at school: Hericium erimaceus (Bull exfr) Pers.
(B) the white mushroom material of Eumycota Basidiomycetes Agaricales Lentinus mushroom
Formal name used at school: Lentinus edodes (Berk) Sing
(C) the white mushroom material of Eumycota Basidiomycetes Agaricales flammule Pseudomonas Asparagus
Formal name used at school: Sing Flammulina velulipes(Fr)
Two, has some micro-characteristic of enrichment according to above-mentioned edible mushroom, adopt the concentration gradient pressure application to tame out selenium, germanium, hericium erinaceus, mushroom bacterium and golden mushroom that the zinc enriching quantity is high, and then in the process of cultivating fruit body, with additive composts or fertilisers of cultivating is handled again, to reach the directed purpose that improves some active ingredient in the above-mentioned edible mushroom with trace element.
Three, according to Hericium erinaceus, the Different Nutrition composition of mushroom and three kinds of raw materials of Asparagus, and human body is to the needs of selenium, germanium and zinc carries out the science assembly with the dry powder of three kinds of raw materials, thereby obtains multielement edible fungus protein body.
Processing step of the present invention is as follows:
The Hericium erinaceus of (1) domestication, cultivation enrichment selenium, germanium, zinc
(1.1) domestication hericium erinaceus
(1.1.1) with Raper and Miles(1958) perfect medium is that minimal medium makes one group of hericium erinaceus minimal medium that is added with sodium selenite, germanium oxide and the zinc sulphate of different amounts, be respectively charged into corresponding one group more in vitro; Prepare the benchmark test tube that Raper and Miles perfect medium are housed simultaneously in addition;
(1.1.2) in above-mentioned test tube, connect hericium mycelium according to a conventional method, and carry out spawn culture, after 20 days, observe relatively test tube and the invisible spectro mycelial growth situation of benchmark, select several mycelial growth situations normal and with the comparison test tube of the invisible spectro mycelia basically identical of benchmark, therefrom select the more comparison test tube of sodium selenite, germanium oxide and zinc sulphate addition in its medium again, determine that the mycelia in this test tube is first generation Hericium erinaceus domestication mycelia;
(1.1.3) allocate medium with sodium selenite, germanium chloride and zinc sulphate additive with reference to the proportioning of additive in the medium of first generation mycelia, first generation Hericium erinaceus is tamed mycelium inoculation on this medium, carry out several times and cultivate domestication repeatedly, thereby obtain to adapt to the high Hericium erinaceus domesticated strain of selenium, germanium, zinc enriching quantity that this medium is grown;
(1.2) Hericium erinaceus of cultivation enrichment selenium, germanium, zinc
(1.2.1) adopt the wheat composts or fertilisers of cultivating
(1.2.2) preparation has the solution of sodium selenite, germanium oxide and zinc sulphate, and the proportioning of its additive is lower than (1.1.3) additive proportioning in order to the medium of domestication first generation mycelia, and to transfer the pH value of this solution be 3～5;
(1.2.3) by the solution with sodium selenite, germanium oxide and zinc sulphate of some kinds of variable concentrations of concentration graded method preparation, and the pH value of transferring above-mentioned solution is 3～5, the solution of above-mentioned some kinds of variable concentrations is by after the mixed in equal amounts, and the concentration of each additive is lower than the solution of (1.2.2) preparation in its intermixture;
(1.2.4) total suction according to dried composts or fertilisers of cultivating is spilled into the some kinds of solution of (1.2.3) preparation in the composts or fertilisers of cultivating successively, makes it abundant absorption;
(1.2.5) will in the solution of composts or fertilisers of cultivating input (1.2.2) preparation that (1.2.4) handles, boil, and take out then and drain accent PH5～6;
(1.2.6), again the hericium erinaceus after (1.1.3) domestication is inserted in the blake bottle, cultivate Hericium erinaceus according to a conventional method according to a conventional method with composts or fertilisers of cultivating bottling, sterilization;
(2) mushroom of acclimatization culture enrichment selenium, germanium
(2.1) domestication mushroom bacterium
(2.1.1) with Paper and Miles(1958) perfect medium is that minimal medium makes one group and is added with the sodium selenite of different amounts and the mushroom bacterium minimal medium of germanium oxide, be respectively charged into corresponding one group more in vitro; Prepare the benchmark test tube that Raper and Miles perfect medium are housed simultaneously in addition;
(2.1.2) in above-mentioned test tube, connect mushroom mycelium according to a conventional method, and carry out spawn culture, after 30 days, observe each relatively test tube and the invisible spectro mycelial growth situation of benchmark, select several mycelial growth situations normal and with the comparison test tube of the invisible spectro mycelia basically identical of benchmark, therefrom select sodium selenite and the more comparison test tube of germanium oxide addition in its medium again, determine that the mycelia in this test tube is first generation mushroom domestication mycelia;
(2.1.3) allocate medium with sodium selenite and germanium oxide additive with reference to the proportioning of additive in the medium of first generation mycelia, first generation mushroom is tamed mycelium inoculation on this medium, carry out several times and cultivate domestication repeatedly, thereby obtain to adapt to the high mushroom domesticated strain of selenium, germanium enriching quantity that this medium is grown;
(2.2) mushroom of cultivation enrichment selenium germanium
(2.2.1) adopt the wheat composts or fertilisers of cultivating
(2.2.2) preparation has the solution of sodium selenite and germanium oxide, and the proportioning of its additive is lower than (2.1.3) additive proportioning in order to the medium of domestication first generation mycelia, and to transfer the pH value of this solution be 3～5;
(2.2.3) by the solution with sodium selenite and germanium oxide of some kinds of variable concentrations of concentration graded method preparation, and the pH value of transferring above-mentioned solution is 3～5, the solution of above-mentioned some kinds of variable concentrations is by after the mixed in equal amounts, and the concentration of each additive is lower than the solution of (2.2.2) preparation in its intermixture;
(2.2.4) total suction according to dried composts or fertilisers of cultivating is spilled into the some kinds of solution of (2.2.3) preparation in the composts or fertilisers of cultivating successively, makes it abundant absorption;
(2.2.5) will in the solution of composts or fertilisers of cultivating input (2.2.2) preparation that (2.2.4) handles, boil, and take out then and drain accent PH5～6;
(2.2.6), again the mushroom bacterium after (2.1.3) domestication is inserted in the blake bottle, cultivated mushroom according to a conventional method according to a conventional method with composts or fertilisers of cultivating bottling, sterilization;
(3) Asparagus of acclimatization culture enrichment selenium, germanium, zinc
(3.1) domestication golden mushroom
(3.1.1) with Raper and Miles(1958) perfect medium is that minimal medium makes one group of golden mushroom minimal medium that is added with sodium selenite, germanium oxide and the zinc sulphate of different amounts, be respectively charged into corresponding one group more in vitro; Prepare the benchmark test tube that Raper and Miles perfect medium are housed simultaneously in addition;
(3.1.2) in above-mentioned test tube, connect the Asparagus mycelia according to a conventional method, and carry out spawn culture, after 20 days, observe each relatively test tube and the invisible spectro mycelial growth situation of benchmark, select several mycelial growth situations normal and with the comparison test tube of the invisible spectro mycelia basically identical of benchmark, therefrom select the more comparison test tube of sodium selenite, germanium oxide and zinc sulphate addition in its medium again, determine that the mycelia in this test tube is first generation Asparagus domestication mycelia.
(3.1.3) allocate medium with sodium selenite, germanium chloride and zinc sulphate additive with reference to the proportioning of additive in the medium of first generation mycelia, first generation Asparagus is tamed mycelium inoculation on this medium, carry out several times and cultivate domestication repeatedly, thereby obtain to adapt to the high Asparagus domesticated strain of selenium, germanium, zinc enriching quantity that this medium is grown;
(3.2) Asparagus of cultivation enrichment selenium, germanium, zinc
(3.2.1) adopt the wheat composts or fertilisers of cultivating
(3.2.2) preparation has the solution of sodium selenite, germanium oxide and zinc sulphate, and the proportioning of its additive is lower than (3.1.3) additive proportioning in order to the medium of domestication first generation mycelia, and to transfer the pH value of this solution be 3～5;
(3.2.3) by the solution with sodium selenite, germanium oxide and zinc sulphate of some kinds of variable concentrations of concentration graded method preparation, and the pH value of transferring above-mentioned solution is 3～5, the solution of above-mentioned some kinds of variable concentrations is by after the mixed in equal amounts, and the concentration of each additive is lower than the solution of (3.2.2) preparation in its intermixture;
(3.2.4) total suction according to dried composts or fertilisers of cultivating is spilled into the some kinds of solution of (3.2.3) preparation in the composts or fertilisers of cultivating successively, makes it abundant absorption;
(3.2.5) will in the solution of composts or fertilisers of cultivating input (3.2.2) preparation that (3.2.4) handles, boil, and take out then and drain accent PH5～6;
(3.2.6), again the golden mushroom after (3.1.3) domestication is inserted in the blake bottle, cultivate Asparagus according to a conventional method according to a conventional method with composts or fertilisers of cultivating bottling, sterilization;
(4) processed finished products
(4.1) Hericium erinaceus of respectively above-mentioned steps being cultivated, mushroom and Asparagus are dried and handle and wear into powder,
(4.2) to be made into edible composition to be multielement edible fungus protein body to three kinds of edible mushroom powdery things that process with above-mentioned steps.
The embodiment concrete grammar of (1.1) domestication hericium erinaceus set by step is:
(1.1.1) with Paper and Miles(1958) perfect medium is minimal medium, by nine kinds of sodium selenites that are added with different amounts of orthogonal experiment preparation, the hericium erinaceus minimal medium of germanium oxide and zinc sulphate, its additive proportioning is respectively:
A
1（1mg/1NaSeO
2+1mg/GeO
2+5mg/1 ZnSO
4）；
A
2（1mg/1NaSeO
2+5mg/GeO
2+10mg/1 ZnSO
4）；
A
3（1mg/1NaSeO
2+10mg/GeO
2+20mg/1 ZnSO
4）；
A
4（5mg/1NaSeO
2+1mg/GeO
2+5mg/1 ZnSO
4）；
A
5（5mg/1NaSeO
2+5mg/GeO
2+10mg/1 ZnSO
4）；
A
6（5mg/1NaSeO
2+10mg/GeO
2+20mg/1 ZnSO
4）；
A
7（10mg/1NaSeO
2+1mg/GeO
2+5mg/1 ZnSO
4）；
A
8（10mg/1NaSeO
2+5mg/GeO
2+10mg/1 ZnSO
4）；
A
9（1mg/10NaSeO
2+10mg/GeO
220mg/1 ZnSO
4）；
Above-mentioned medium is respectively charged in vitro corresponding, the medium of every kind of concentration is adorned 10 test tubes, simultaneously 10 benchmark test tubes that Raper and Miles perfect medium are housed of preparation in addition.
(1.1.2) select the hericium mycelium of robust growth, be inoculated in respectively on the inclined-plane of medium of above-mentioned various test tubes, place 25 ℃ then, the low light level is cultivated down, cultivates after 20 days, takes out the mycelial growth situation of the various processing of observation, and the result shows A
1, A
2, A
4, and A
5, four processing mycelial growth and benchmark test tube basically identical, i.e. mycelia physical efficiency normal growth, its growth rate is also identical with contrast.Wherein, A
1And A
4The mycelial growth of handling is the most consistent with the benchmark test tube, determines A
4The mycelia of handling is first generation Hericium erinaceus domestication mycelia (remaining each processing, mycelial growth is bad, it is slow to show as growth rate, the mycelia scale of construction is few, proves that its growth is suppressed to a certain extent).
(1.1.3) subsequently, to A
4The proportioning of the additive of handling is done further to adjust, and gets A
10(3mg/1NaSeO
2+ 2mg/1GeO
2+ 6mg/1 ZnSO
4) get A
4The mycelium inoculation of handling is in through A
10Again cultivate on the medium of handling, thereby obtain normal, the stable bacterial classification of mycelial growth in this medium.This bacterial classification is the hedgehog fungus bacterial of high concentration enrichment selenium, germanium and zinc.(carry out the test of conventional mushroom producing culture with this bacterial classification, as a result characteristics such as its mushroom shape, color and luster all with to contrast former mushroom consistent).
The embodiment method of (1.2) cultivation Hericium erinaceus set by step is:
(1.2.1) adopt the wheat composts or fertilisers of cultivating, the prescription of wheat composts or fertilisers of cultivating is:
Barley 90～98%; Calcium carbonate 1～2%; Other dressing 1～5%.
(1.2.2) preparation has the solution of sodium selenite, germanium oxide and zinc sulphate, in the solution proportioning of additive be in order to the medium of domestication first generation mycelia 1/2 promptly according to A
10The proportioning of additive in the medium of handling is pressed the proportioning that 1/2 ratiometric conversion becomes the additive of the obtain solution of wanting, and the conversion result: sodium selenite is 1.5mg/kg; Germanium oxide is 1mg/kg; Zinc sulphate is 3mg/kg.
(1.2.3) fully absorb sodium selenite, germanium chloride and three kinds of additives of zinc sulphate from the inside to the outside gradually, earlier by four kinds of solution of concentration graded method preparation in order to help composts or fertilisers of cultivating; After promptly earlier three kinds of interpolations being dissolved respectively, mix the fixed solution that is mixed with four concentration levels that becomes again,
a
1(0.1ppm NaSeO
2+0.1ppm GeO
2+0.3ppm ZnSO
4）；
a
2(0.3ppm NaSeO
2+0.2ppm GeO
2+0.5ppm ZnSO
4）；
a
3(0.4ppm NaSeO
2+0.3ppm GeO
2+1.0ppm ZnSO
4）；
a
4(0.7ppm NaSeO
2+0.4ppm GeO
2+1.2ppm ZnSO
4）；
Four kinds of equal furnishing PH3-5 of solution
(1.2.4) test is calculated then, the water absorption of the composts or fertilisers of cultivating that per kilogram is done, thereby definite amount of adding four kinds of solution successively.As, the dried composts or fertilisers of cultivating of per kilogram absorbs 0.8 kilogram water, and the total amount of then adding above-mentioned four kinds of solution is 0.8 kilogram (800ml).Concrete grammar is (is example with 1 kilogram of dried composts or fertilisers of cultivating): use a earlier
1Solution 200ml is sprayed on the dried composts or fertilisers of cultivating (composts or fertilisers of cultivating need stir) in the mode of spraying and uses a after 2-3 hour
2Solution 200ml sprays, and after 3-4 hour, uses a
3Solution 200ml sprays, and after 5-6 hour, uses a
4Solution 200ml soaks, and evenly stirs composts or fertilisers of cultivating, until blotting a
4Till the solution.
(1.2.5) subsequently, the composts or fertilisers of cultivating of above-mentioned processing boiled with solution of preparation in excessive (1.2.2) boil 5-10 minute, take out to pick up and drain, the accent pH value is 5-6.
(1.2.6) bottle according to a conventional method, sterilization, last in inoculating hood, will insert in the blake bottle through the hedgehog fungus bacterial of the enrichment selenium of domestication, germanium, zinc.Place under 25 ℃ of left and right sides low light conditions and cultivate.
The embodiment method of (2.1) domestication mushroom bacterium set by step is:
(2.1.1) with Paper and miles(1958) perfect medium is minimal medium, is added with the sodium selenites of different amounts and the mushroom bacterium minimal medium of germanium oxide for four kinds by the orthogonal experiment preparation, its additive proportioning is respectively:
B
1（1mg/1 NaSeO
2+1mg/1 GeO
2
B
2（1mg/1 NaSeO
2+5mg/1 GeO
2
B
3（5mg/1 NaSeO
2+1mg/1 GeO
2
B
4（5mg/1 NaSeO
2+5mg/1 GeO
2
Above-mentioned medium is respectively charged in vitro corresponding, the medium of every kind of concentration is adorned 10 test tubes.Simultaneously, prepare 10 in addition the benchmark test tube that Paper and Miles cultivate fully is housed.
(2.1.2) mycelia of getting mushroom strain is inoculated in vitro above-mentionedly respectively, places about 25 ℃, cultivates under the dark condition, cultivates to take out after 30 days and observes, as a result B
1And B
3The mycelial growth of handling is similar to the benchmark test tube, determines B
3Handle mycelia and be first generation mushroom domestication mycelia.(B
2And B
4The mycelial growth amount less slightly, it is isabelline that mycelia slightly is).
(2.1.3) to B
3The proportioning of additive adjust, B
5(4mg/1 NaSeO
2+ 2mg/1 GeO
2), B
3The mycelia of handling is transferred to through B
5Continue on the medium of handling to cultivate, use through B
5The medium of handling carries out 3-5 time the cultivation of domestication repeatedly, thereby obtain normal, the stable bacterial classification of mycelial growth in this medium, this bacterial classification is the mushroom strain (this bacterial classification passes through conventional fruiting experiment equally, does not have the anomaly of discovery) of high concentration enrichment selenium, germanium.
The embodiment method of the mushroom of (2.2) cultivation enrichment selenium, germanium set by step is:
(2.2.1) identical with embodiment step (1.2.1):
(2.2.2) preparation has the solution of sodium selenite and germanium oxide, and the proportioning of additive is in order to 1/2 of the medium of domestication first generation mycelia, promptly according to B in the solution
5The proportioning of additive in the medium of handling is pressed the proportioning that 1/2 ratiometric conversion becomes the additive of the obtain solution of wanting, and the conversion result: sodium selenite is 2mg/l; Germanium oxide is 1mg/l;
(2.2.3) earlier sodium selenite and germanium oxide are dissolved respectively after, mix the solution that constant volume is mixed with four concentration levels again:
b
1（0.2 NaSeO
2+0.1 GeO
2
b
2（0.5 NaSeO
2+0.2 GeO
2
b
3（0.6 NaSeO
2+0.3 GeO
2
b
4（0.7 NaSeO
2+0.4 GeO
2
Four kinds of equal furnishing PH3-5 of solution.
(2.2.4) test is calculated then, the water absorption of the composts or fertilisers of cultivating that per kilogram is done, thereby definite amount of adding four kinds of solution successively.As, the dried composts or fertilisers of cultivating of per kilogram absorbs 0.8 kilogram water, and the total amount of then adding above-mentioned four kinds of solution is 0.8 kilogram (800ml).Concrete grammar is (1 kilogram of dried composts or fertilisers of cultivating is an example): after 2-3 hour, 200ml sprays with b solution, after 3-4 hour, uses b earlier to be sprayed on the dried composts or fertilisers of cultivating (composts or fertilisers of cultivating need stir) with b solution 200ml in the mode of spraying
3Solution 200ml sprays, and after 5-6 hour, uses b
4Solution 200ml soaks, and evenly stirs composts or fertilisers of cultivating, until blotting b
4Till the solution.
(2.2.5) subsequently, the composts or fertilisers of cultivating of above-mentioned processing boiled with solution of preparation in excessive (2.2.2) boil 5-10 minute, take out to pick up and drain, the accent pH value is 5-6.
(2.2.6) bottle routinely, sterilization, the last blake bottle that will insert through the mushroom strain of the enrichment selenium of domestication, germanium in inoculating hood is built under 25 ℃ of left and right sides low light conditions and cultivates.
The embodiment method of (3.1) domestication golden mushroom set by step is:
(3.1.1) identical with (1.1.1)
(3.1.2) select the golden mushroom mycelium of robust growth, be connected to respectively on the inclined-plane of medium of above-mentioned various test tubes, place 20 ℃ then, dark condition is cultivated down, cultivates after 20 days, takes out the mycelial growth situation of the various processing of observation, and the result shows A
1, A
2, A
4, A
5And A
7, mycelial growth and the benchmark test tube basically identical handled, determine A
5The mycelia of handling is first generation Asparagus domestication mycelia.
(3.1.3) subsequently, to A
5The proportioning of the additive of handling is done further to adjust, and gets A
11(4mg/1 NaSeO
2+ 4mg/1 GeO
2+ 8mg/1 ZnSO
4) get A
5The mycelium inoculation of handling is in through A
11Again cultivate on the medium of handling, use through A
11The medium of handling carries out 3-5 time the cultivation of domestication repeatedly, thereby obtains normal, the stable bacterial classification of mycelial growth in this medium.This bacterial classification is the Asparagus bacterial classification of high concentration enrichment selenium, germanium and zinc.(carry out the test of conventional mushroom producing culture with this bacterial classification, characteristics such as its mushroom shape, color and luster are all with to contrast former mushroom consistent as a result.）
The embodiment method of (3.2) cultivation Asparagus set by step is:
(3.2.1) identical with (1.2.1):
(3.2.2) preparation has the solution of sodium selenite, germanium oxide and zinc sulphate, and the proportioning of additive is in order to 1/2 of the medium of domestication first generation mycelia, promptly according to A in the solution
11The proportioning of additive in the medium of handling is pressed the proportioning that 1/2 ratiometric conversion becomes the additive of the obtain solution of wanting, and the conversion result: sodium selenite is 2mg/l; Germanium oxide is 2mg/l; Zinc sulphate is 4mg/l.
(3.2.3) fully absorb sodium selenite, germanium oxide and three kinds of additives of zinc sulphate from the inside to the outside gradually, earlier by four kinds of solution of concentration graded method preparation in order to help composts or fertilisers of cultivating; After promptly earlier three kinds of additives being dissolved respectively, mix the solution that constant volume is mixed with four concentration levels again,
c
1（0.2ppm NaSeO
2+0.2ppm GeO
2+0.4ppm ZnSO
4）；
c
2（0.4ppm NaSeO
2+0.4ppm GeO
2+0.8ppm ZnSO
4）；
c
3（0.6ppm NaSeO
2+0.6ppm GeO
2+1.2ppm ZnSO
4）；
c
4（0.8ppm NaSeO
2+0.8ppm GeO
2+1.6ppm ZnSO
4）；
Four kinds of equal furnishing PH3-5 of solution
(3.2.4) water absorption of the composts or fertilisers of cultivating that test calculating per kilogram is dried then, thereby definite amount of adding four kinds of solution successively.As, the dried composts or fertilisers of cultivating of per kilogram absorbs 0.8 kilogram (800ml).Concrete grammar is (1 kilogram of dried composts or fertilisers of cultivating is an example): after 2-3 hour, 200ml sprays with c solution, after 3-4 hour, uses c earlier to be sprayed on the dried composts or fertilisers of cultivating (composts or fertilisers of cultivating need stir) with c solution 200ml in the mode of spraying
3Solution 200ml sprays, and after 5-6 hour, uses c
4Solution 200ml soaks, and evenly stirs composts or fertilisers of cultivating, until blotting c
4Till the solution.
(3.2.5) subsequently, the composts or fertilisers of cultivating of above-mentioned processing boiled with solution of preparation in excessive (3.2.2) boil 5-10 minute, take out to pick up and drain, the accent pH value is 5-6.
(3.2.6) bottle routinely, sterilization, last in inoculating hood, will insert blake bottle and be built in about 26 ℃ through the Asparagus bacterial classification of the enrichment selenium of domestication, germanium, zinc, dark condition is cultivated down.
The prescription of composition that can be edible by (4.2) preparation among the embodiment is: Hericium erinaceus powder 10-30%; Mushroom powder 30-50%; Golden needle fungus 30-50%.
According to the Hericium erinaceus of being cultivated, the Different Nutrition composition of mushroom and Asparagus and human body are to the needs of selenium, germanium, zinc, and above-mentioned composition is that the optimum formula of multielement edible fungus protein body is: Hericium erinaceus powder 20%, mushroom powder 40%; Golden needle fungus 40%.
The analytical test report (seeing Appendix 1) that product multielement edible fungus protein body of the present invention detects through Fujian Province test center shows, the amino acid A wide selection of colours and designs that this product is not only contained, and be rich in multiple trace element to the human body beneficial, particularly the content of selenium, germanium and zinc is the most outstanding.Wherein the content of selenium is that 2mg/100g is 37 times of mushroom selenium content, and the content of germanium is 2.82mg/100g, is 10 times of the germanic amount of Wild ganoderma.The chemistry of the contained selenium of this product constituted carry out assay determination, the result shows, 99.8% exists (seeing Appendix 2) with the organic form
The three kinds of trace elements of selenium, germanium, zinc that are rich in this polynary vegetarian diet mycoprotein body, generally acknowledged promoting the metabolism of human body, raise immunity and anti-ageingly have an obvious effect, and they exist with organic form and easily are absorbed by the body in a certain amount of scope the not any side effect of tool.
The being rich in nutrition value of product one multielement proteosome of the present invention can be processed into the nutriment capsule, beverage, food additives or special medicine as required.
Claims (4)
1, a kind of preparation method of multielement edible fungus protein body is characterized in that this preparation method's step is as follows:
The Hericium erinaceus of (1) domestication, cultivation enrichment selenium, germanium, zinc
(1.1) domestication hericium erinaceus
Be that minimal medium makes one group of hericium erinaceus minimal medium that is added with sodium selenite, germanium oxide and the zinc sulphate of different amounts with Miles (1958) perfect medium (1.1.1) with Raper, be respectively charged into corresponding one group more in vitro; Prepare the benchmark test tube that Raper and Miles perfect medium are housed simultaneously in addition;
(1.1.2) in above-mentioned test tube, connect hericium mycelium according to a conventional method, and carry out spawn culture, after 20 days, observe each relatively test tube and the invisible spectro mycelial growth situation of benchmark, select several mycelial growth situations normal and with the comparison test tube of the invisible spectro mycelia basically identical of benchmark, therefrom select the more comparison test tube of sodium selenite, germanium oxide and zinc sulphate addition in its medium again, determine that the mycelia in this test tube is first generation Hericium erinaceus domestication mycelia;
(1.1.3) allocate medium with sodium selenite, germanium chloride and zinc sulphate additive with reference to the proportioning of additive in the medium of first generation mycelia, first generation Hericium erinaceus is tamed mycelium inoculation on this medium, carry out several times and cultivate domestication repeatedly, thereby obtain to adapt to the high Hericium erinaceus domesticated strain of selenium, germanium, zinc enriching quantity that this medium is grown;
(1.2) Hericium erinaceus of cultivation enrichment selenium, germanium, zinc
(1.2.1) adopt the wheat composts or fertilisers of cultivating
(1.2.2) preparation has the solution of sodium selenite, germanium oxide and zinc sulphate, and the proportioning of its additive is lower than (1.1.3) additive proportioning in order to the medium of domestication first generation mycelia, and to transfer the pH value of this solution be 3～5;
(1.2.3) by the solution with sodium selenite, germanium oxide and zinc sulphate of some kinds of variable concentrations of concentration graded method preparation, and the pH value of transferring above-mentioned solution is 3～5, the solution of above-mentioned some kinds of variable concentrations is by after the mixed in equal amounts, and the concentration of each additive is lower than the solution of (1.2.2) preparation in its intermixture;
(1.2.4) total suction according to dried composts or fertilisers of cultivating is spilled into the some kinds of solution of (1.2.3) preparation in the composts or fertilisers of cultivating successively, makes it abundant absorption;
(1.2.5) will in the solution of composts or fertilisers of cultivating input (1.2.2) preparation that (1.2.4) handles, boil, and take out then and drain accent PH5～6;
(1.2.6), again the hericium erinaceus after (1.1.3) domestication is inserted in the blake bottle, cultivate Hericium erinaceus according to a conventional method according to a conventional method with composts or fertilisers of cultivating bottling, sterilization;
(2) mushroom of acclimatization culture enrichment selenium, germanium
(2.1) domestication mushroom bacterium
Be that minimal medium makes one group and is added with the sodium selenite of different amounts and the mushroom bacterium minimal medium of germanium oxide (2.1.1) with Paper and Miles (1958) perfect medium, be respectively charged into corresponding one group more in vitro; Prepare the benchmark test tube that Raper and Miles perfect medium are housed simultaneously in addition;
(2.1.2) in above-mentioned test tube, connect mushroom mycelium according to a conventional method, and carry out spawn culture, after 30 days, observe each relatively test tube and the invisible spectro mycelial growth situation of benchmark, select several mycelial growth situations normal and with the comparison test tube of the invisible spectro mycelia basically identical of benchmark, therefrom select sodium selenite and the more comparison test tube of germanium oxide addition in its medium again, determine that the mycelia in this test tube is first generation mushroom domestication mycelia;
(2.1.3) allocate medium with sodium selenite and germanium oxide additive with reference to the proportioning of additive in the medium of first generation mycelia, first generation mushroom is tamed mycelium inoculation on this medium, carry out several times and cultivate domestication repeatedly, thereby obtain to adapt to the high mushroom domesticated strain of selenium, germanium enriching quantity that this medium is grown;
(2.2) mushroom of cultivation enrichment selenium germanium
(2.2.1) adopt the wheat composts or fertilisers of cultivating
(2.2.2) preparation has the solution of sodium selenite and germanium oxide, and the proportioning of its additive is lower than (2.1.3) additive proportioning in order to the medium of domestication first generation mycelia, and to transfer the pH value of this solution be 3～5;
(2.2.3) by the solution with sodium selenite and germanium oxide of some kinds of variable concentrations of concentration graded method preparation, and the pH value of transferring above-mentioned solution is 3～5, the solution of above-mentioned some kinds of variable concentrations is by after the mixed in equal amounts, and the concentration of each additive is lower than the solution of (2.2.2) preparation in its intermixture;
(2.2.4) total suction according to dried composts or fertilisers of cultivating is spilled into the some kinds of solution of (2.2.3) preparation in the composts or fertilisers of cultivating successively, makes it abundant absorption;
(2.2.5) will in the solution of composts or fertilisers of cultivating input (2.2.2) preparation that (2.2.4) handles, boil, and take out then and drain accent PH5～6;
(2.2.6), again the mushroom bacterium after (2.1.3) domestication is inserted in the blake bottle, cultivated mushroom according to a conventional method according to a conventional method with composts or fertilisers of cultivating bottling, sterilization;
(3) Asparagus of acclimatization culture enrichment selenium, germanium, zinc
(3.1) domestication golden mushroom
Be that minimal medium makes one group of golden mushroom minimal medium that is added with sodium selenite, germanium oxide and the zinc sulphate of different amounts with Miles (1958) perfect medium (3.1.1) with Raper, be respectively charged into corresponding one group more in vitro; Prepare the benchmark test tube that Raper and Miles perfect medium are housed simultaneously in addition;
(3.1.2) in above-mentioned test tube, connect the Asparagus mycelia according to a conventional method, and carry out spawn culture, after 20 days, observe each relatively test tube and the invisible spectro mycelial growth situation of benchmark, select several mycelial growth situations normal and with the comparison test tube of the invisible spectro mycelia basically identical of benchmark, therefrom select the more comparison test tube of sodium selenite, germanium oxide and zinc sulphate addition in its medium again, determine that the mycelia in this test tube is first generation Asparagus domestication mycelia;
(3.1.3) allocate medium with sodium selenite, germanium chloride and zinc sulphate additive with reference to the proportioning of additive in the medium of first generation mycelia, first generation Asparagus is tamed mycelium inoculation on this medium, carry out several times and cultivate domestication repeatedly, thereby obtain to adapt to the high Asparagus domesticated strain of selenium, germanium, zinc enriching quantity that this medium is grown;
(3.2) Asparagus of cultivation enrichment selenium, germanium, zinc
(3.2.1) adopt the wheat composts or fertilisers of cultivating
(3.2.2) preparation has the solution of sodium selenite, germanium oxide and zinc sulphate, and the proportioning of its additive is lower than (3.1.3) additive proportioning in order to the medium of domestication first generation mycelia, and to transfer the pH value of this solution be 3～5;
(3.2.3) by the solution with sodium selenite, germanium oxide and zinc sulphate of some kinds of variable concentrations of concentration graded method preparation, and the pH value of transferring above-mentioned solution is 3～5, the solution of above-mentioned some kinds of variable concentrations is by after the mixed in equal amounts, and the concentration of each additive is lower than the solution of (3.2.2) preparation in its intermixture;
(3.2.4) total suction according to dried composts or fertilisers of cultivating is spilled into the some kinds of solution of (3.2.3) preparation in the composts or fertilisers of cultivating successively, makes it abundant absorption;
(3.2.5) will in the solution of composts or fertilisers of cultivating input (3.2.2) preparation that (3.2.4) handles, boil, and take out then and drain accent PH5～6;
(3.2.6), again the golden mushroom after (3.1.3) domestication is inserted in the blake bottle, cultivate Asparagus according to a conventional method according to a conventional method with composts or fertilisers of cultivating bottling, sterilization;
(4) processed finished products
(4.1) Hericium erinaceus of respectively above-mentioned steps being cultivated, mushroom and Asparagus are dried and handle and wear into powder,
(4.2) to be made into edible composition to be multielement edible fungus protein body to three kinds of edible mushroom powdery things that process with above-mentioned steps.
2, the preparation method of multielement edible fungus protein body according to claim 1 is characterized in that: the prescription of the composition in the step (4.2) is: Hericium erinaceus powder 10-20%; Mushroom powder 30-50%; Golden needle fungus 30-50%; Step (1.2.1) (2.2.1) the wheat composts or fertilisers of cultivating prescription in (3.2.1) is barley 90-98%; Calcium carbonate 1-2%; Other dressing 1-5%.
3, the preparation method of multielement edible fungus protein body according to claim 1 is characterized in that:
Step (1.1.1) is to make one group of minimal medium that is added with sodium selenite, germanium oxide and the zinc sulphate of different amounts by the text test method(s) (3.1.1); Step (2.1.1) is to make one group by orthogonal experiment to be added with the sodium selenite of different amounts and the minimal medium of germanium oxide; Step (1.2.2) (2.2.2) and (3.2.2) in the solution proportioning of additive be respectively that (1.1.3) is (2.1.2) and (3.1.3) in order to 1/2 of the medium of domestication first generation mycelia.
4, according to the preparation method of claim 1,2 or 3 described multielement edible fungus protein bodies, it is characterized in that: the prescription of the composition in the step (4.2) is: Hericium erinaceus powder 20%, mushroom powder 40%, golden needle fungus 40%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93114635 CN1100587A (en) | 1993-11-11 | 1993-11-11 | Method for prepn. of multi-element edible fungus protein |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93114635 CN1100587A (en) | 1993-11-11 | 1993-11-11 | Method for prepn. of multi-element edible fungus protein |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6737065B2 (en) * | 2000-10-06 | 2004-05-18 | Jae-Mahn Song | Method for producing mushroom mycelia and uses thereof |
| CN1799317B (en) * | 2005-12-30 | 2010-07-21 | 叶华福 | Method for cultivating natural white striped mushroom |
| CN101829159A (en) * | 2010-06-03 | 2010-09-15 | 樊美珍 | Method for preparing selenium-enriched monkey head mushroom capsule |
| CN101433328B (en) * | 2008-12-29 | 2013-06-19 | 北京玛西蒙科技有限公司 | Method for producing fresh Hericium erinaceus complete-matter nourishing powder |
-
1993
- 1993-11-11 CN CN 93114635 patent/CN1100587A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6737065B2 (en) * | 2000-10-06 | 2004-05-18 | Jae-Mahn Song | Method for producing mushroom mycelia and uses thereof |
| CN1799317B (en) * | 2005-12-30 | 2010-07-21 | 叶华福 | Method for cultivating natural white striped mushroom |
| CN101433328B (en) * | 2008-12-29 | 2013-06-19 | 北京玛西蒙科技有限公司 | Method for producing fresh Hericium erinaceus complete-matter nourishing powder |
| CN101829159A (en) * | 2010-06-03 | 2010-09-15 | 樊美珍 | Method for preparing selenium-enriched monkey head mushroom capsule |
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