CN1810081A - Liquid mushroom producing method and fermenting set - Google Patents

Liquid mushroom producing method and fermenting set Download PDF

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CN1810081A
CN1810081A CNA2006100183991A CN200610018399A CN1810081A CN 1810081 A CN1810081 A CN 1810081A CN A2006100183991 A CNA2006100183991 A CN A2006100183991A CN 200610018399 A CN200610018399 A CN 200610018399A CN 1810081 A CN1810081 A CN 1810081A
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liquid
medium
porous
gill fungus
concentrator bowl
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CN100513549C (en
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文红涛
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文红涛
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Abstract

The present invention provides one kind of liquid mushroom producing method and fermenting set. The mixture of solid culture medium or skeleton material culture medium and mushroom seed is set in the porous centrifugal barrel inside mushroom fermenting set to ferment for 2-3 days, liquid culture medium is set in the liquid storage and added to the porous centrifugal barrel via a porous liquid conveying pipe, and the porous centrifugal barrel is rotated with the motor to replenish nutrients and oxygen to the culture medium while carrying the excessive culture liquid and fermented matter to outside of the centrifugal barrel and returning to the liquid storage. The present invention has the circulation period of 0.5-1.0 hr and culture period of 2-3 days.

Description

Liquid bacterium gill fungus production method and liquid bacterium gill fungus fermentation equipment thereof
Technical field
The present invention relates to a kind of liquid bacterium gill fungus production method and liquid bacterium gill fungus fermentation equipment thereof, belong to food (medicine) and use the bacterium production field.
Background technology
The bacterium gill fungus is a microorganism, is the macro fungi of forming in the fungi of microbiology class of one of natural three mcroorganism colonies.Set up and developing agricultural microorganism industry, and realization industrialization, i.e. " white agriculture ", be that chief Bao Jianzhong researcher at first initiates before the biological control research institute of the Chinese Academy of Agricultural Sciences, China is the birthplace of " white agriculture ", and famous scientist's Qian Xuesen comrade estimates and sets up and develop " white agriculture " is the beginning of the 6th Industrial Revolution in China.
Scientists prophesy: human attention to food once transferred amino acid to by glucose, and 21 century will transfer active polysaccharide to by amino acid, and the active polysaccharide production product of macro fungi just; Being of high nutritive value of bacterium gill fungus, lack campaign, do not occupy cultivated land, need not apply fertilizer, do not need sunlight, water is few, therefore, many scientists' suggestions, the African people's protein source is preferably solved by macro fungi from now on.
The product of Edible Fungi all is a fruit body both at home and abroad now, is commonly called as mushroom or mushroom.The fruit body of edible mushroom is equivalent to the fruit of plant, and many edible mushrooms manually can be turned out mycelium now, but can't grow fruit body, thereby can not put into production.Fruit body is as product, and major part all is a marketing fresh, but the fresh-keeping difficulty of fruit body, and the fresh-keeping time is short, have under natural temperature in addition can only fresh-keeping several hrs; Next is a dry product, and dry product has not only increased production cost, but also can lose original local flavor, has lost nutrient component; Be to make can salt marsh product once more, the situation of salt marsh product is similar to dry product; Minority is as the leaching liquor of then using fruit body of health products or medicine, and leaching liquor one is that recovery rate is low, and the 2nd, also to lose many useful compositions.
The bacterium gill fungus comprises edible mushroom, medicinal fungus, edible mushroom such as mushroom, Agaricus Bisporus, straw mushroom, Asparagus, flat mushroom, willow mushroom, white fungus, woodear, Agaricus blazei Murrill, Hericium erinaceus, hickory chick, grifola frondosus etc.; Medicinal fungus such as glossy ganoderma, rhizoma Gastrodiae, Poria cocos, Chinese caterpillar fungus etc.; Actual many edible mushrooms all have health care, medical functions, and medicinal fungus has food function equally, and both are difficult to well-separated.Therefore, many scholars are referred to as edible mushroom, and perhaps rare uses bacterium for food (medicine), have medicine, two kinds of functions of food simultaneously concurrently, are the characteristics of bacterium gill fungus.
Present Edible Fungi both domestic and external is solids manufacture all, and the raw material of just producing the medium of edible mushroom is solid with the medium of working good, and raw material mainly is a timber, and China also uses cotton seed hulls in a large number, and the stalk of crops.Solids manufacture raw material consumption is big, need that production site is big, the production cycle long, " waste residue " after producing is many.
The Edible Fungi technology overwhelming majority of China is to adopt " cultivating in bag ", serves as to produce container with plastic sack or plastic bottle promptly.This production technology is an important breakthrough to the Edible Fungi technology, but has also brought many new problems simultaneously.Wherein the most outstanding is, because postvaccinal fermentation is to carry out in the plastic containers of basic sealing, so: the one, during sending out bacterium (medium fermentation), except that temperature, other fermentation condition such as water content, nutrient component, acid degree of subtracting etc., people are difficult to regulation and control.The 2nd, the oxygen supply difficulty.
Submerged fermentation is a kind of liquid fermentation mode that some industrial microorganism and medical microorganism are adopted, and fermentation is to carry out in liquid, but the product majority neither be liquid.
Though submerged fermentation has its advantage, the expert is consistent both at home and abroad thinks, submerged fermentation also is the direction of development to Edible Fungi at present.Because, the one, specification requirement height, equipment complexity, investment are big, and the 2nd, also have some technical problems to solve, thereby developing direction just.
The greatest problem of submerged fermentation is exactly the supply of oxygen.Aerobic microorganism is placed in the few water of oxygen content cultivates, the oxygen supply difficulty is the congenital deficiency of this technology path.In order to solve the supply problem of oxygen, production equipment also must increase a lot of auxiliary devices such as air compressor, compressed-air actuated cooling, oil removing, sterilization except fermentation tank, both increased the complexity of technology greatly, has increased investment again greatly.Edible mushroom is an aerobic microorganism, to the requirement height of oxygen supply, it is placed in the water of anoxic cultivates, and oxygen supply is maximum contradiction.
The submerged fermentation technology also has a shortcoming that is difficult to overcome, and the equipment that it is used is the fermentation tank of sealing.In order to increase output, to expand the scale of production, what people adopted is the way that strengthens the fermentation tank volume, but, when the diameter of fermentation tank increases, the area of section of jar is by a square increase, and the volume of jar is by a cube increase, per unit liquid just diminishes with the ratio in cross section like this, the diameter of jar increases bigger, per unit liquid is just little with the ratio in cross section, the probability that liquid contacts with gas is just little, and the contradiction of oxygen supply is just more outstanding, and the aerobic microorganism carries out the problem that submerged fermentation is not broken away from fermentation tank like this.And the bacterium gill fungus just in time is the aerobic microorganism.
At present submerged fermentation equipment is except fermentation tank, and also having a kind of is exactly shaking table, and its structure is simple relatively, invests also lessly, but because of its productivity is low, once can only produce the hundreds of milliliter, and maximum several liters are mainly used in breadboard research work.And fermentation tank has yet just begun to be applied to the bacterial classification production of edible mushroom, jar volume also have only several decaliters, do not comprise the corollary equipment price, at least also more than ten thousand yuan, simultaneously that is that all right is ripe for technology.
Summary of the invention
The objective of the invention is to provide a kind of liquid bacterium gill fungus production method and liquid bacterium gill fungus fermentation equipment thereof, solving this critical period people of fermenting when the bacterium gill fungus is cultivated can not regulate and control the moisture of fermentation, nutrition supply, acid-base value etc., and the contradiction of oxygen supply difficulty, technology is simple, equipment volume is little, easy to control, be applicable to that needs are produced in the industrialization of liquid bacterium gill fungus.
Technical scheme of the present invention: liquid bacterium gill fungus production method of the present invention comprises culture propagation, connects bacterial classification, fermentation in medium, and connecing bacterial classification and fermentation in medium is to carry out in liquid bacterium gill fungus fermentation equipment, the steps include:
1) puts into the solid culture medium of process sterilization or the skeleton material medium that the skeleton material mixes with the total nutrient liquid medium in the porous concentrator bowl in bacterium gill fungus fermentation equipment body, with the bacterial classification uniform mixing, bacterial classification accounts for 8%~20% of medium, constantly add oxygen by the air admission hole on the body, fermented 2~3 days;
2) in the liquid reserve tank on the liquid bacterium gill fungus fermentation equipment liquid nutrient medium or total nutrient liquid medium are housed, by the porous woven hose liquid nutrient medium or total nutrient liquid medium are joined in the solid culture medium or skeleton material medium in the porous concentrator bowl, actuating motor drives the rotation of porous concentrator bowl simultaneously, the effect of stirring is played in the rotation of porous concentrator bowl, make liquid nutrient medium or total nutrient liquid medium pass solid culture medium or skeleton material medium, extra-nutrition thing and oxygen take excess liquid culture fluid and fermentation product in the outer body of concentrator bowl to by centrifugal force simultaneously; Liquid nutrient medium that adds or total nutrient liquid medium are polity's medium or skeleton material medium 4~5 times;
3) per 0.5~1.0 hour, the liquid medium in the body and fermentation product pump to liquid reserve tank, are joined in the porous concentrator bowl by the porous woven hose again, actuating motor drives the rotation of porous concentrator bowl once simultaneously, circulates repeatedly; Cultivate again and promptly finished in 2~3 days;
4) the liquid-solid mixed culture fermentation product in the body is emitted from the tap hole of underpart.
Described liquid bacterium gill fungus production method, solid culture medium comprises agricultural product particle, wheat bran, dusty yeast and traditional Chinese medicine.
Skeleton material medium comprises skeleton material and total nutrient liquid medium, and the skeleton material is the graininess skeleton material of nontoxic, water-fast particle greater than the hole of porous concentrator bowl sidewall; The total nutrient liquid medium comprises slurry, sugar, mineral salt, organic carbon, organic nitrogen, the enzyme of crops.
Liquid nutrient medium comprises slurry, sugar, the mineral salt of crops.
Be used for the liquid bacterium gill fungus fermentation equipment that liquid bacterium gill fungus produces, comprise charging aperture on body, the body and the discharging opening under the body, it is characterized in that: be provided with the porous concentrator bowl in the body, the built-in porous woven hose of porous concentrator bowl, the top of porous woven hose is passed body upper and is communicated with liquid reserve tank, body upper has air admission hole and air vent, axle below the porous concentrator bowl passes organism bottom and is connected with driving mechanism, underpart has loop exit to be connected with infusion pump, and infusion pump is connected with liquid reserve tank or porous woven hose by pipeline.
Advantage of the present invention:
One, liquid bacterium gill fungus product has following characteristics:
1, liquid bacterium gill fungus is a liquid condition, is the neomorph of edible fungus.
2, liquid bacterium gill fungus is liquid condition, the utilization that is absorbed by the body easily of relevant composition.
3, the production of liquid bacterium gill fungus does not have discarded object substantially, produces to meet environmental protection requirement.
4, the production of liquid bacterium gill fungus is to carry out in the equipment, and manufacturing condition is fully by Artificial Control, favourable realization batch production, modern production, and the production cycle shorten dramatically.
5, the production of liquid bacterium gill fungus can keep whole active ingredients and the nutrient component of bacterium gill fungus.For example liquid glossy ganoderma had both kept active ingredients such as the polysaccharide, ganoderic acid, triterpene, mineral element, trace element of glossy ganoderma, had also kept nutrient components such as each seed amino acid, vitamin, biology enzyme.
6, the production of liquid bacterium gill fungus can be by the selection to culture medium raw material, the one, not only can effectively regulate and control the composition of product, the 2nd, can also accomplish " enjoying a double blessing ", both kept whole active ingredients and the nutrient component of bacterium gill fungus, also kept the whole active ingredients and the nutrient component of medium.For example produce liquid glossy ganoderma, in the raw material of medium, add soya bean, both kept whole active ingredients and the nutrient component of glossy ganoderma, also kept whole active ingredients and the nutrient component of soya bean.
7, liquid bacterium gill fungus can be according to the requirement of human nutrition balance, and the mixing production that differential diagnosis in tcm is offered free medical treatment, the principle of the monarch and his subjects' compatibility is carried out two kinds of different bacterium gill fungus, or two kinds of liquid bacterium gill fungus are mixed on request, with function and the effect of adjusting it.
8, liquid bacterium gill fungus can also have purpose to add traditional Chinese medicine in medium except the function and effect of giving full play to the bacterium gill fungus, thereby increases and strengthen effect and the effect of liquid bacterium gill fungus.As in medium, adding matrimony vine, thereby make liquid bacterium gill fungus have some functions and the effect of matrimony vine simultaneously.
9, the production of liquid bacterium gill fungus is favourable gives full play to the bacterium gill fungus to rich effect of the collection of trace element and the effect of organising, to improve and the performance effect of trace elements.As to organising of the necessary trace elements of selenium of human body etc.
Two, the characteristics of production technology:
The production technology of liquid bacterium gill fungus both had been different from existing food (medicine) with the solid culture method that bacterium produces, and also was different from tank fermentation method, but both comprehensive a kind of new technologies.
1, the solid, liquid combination is adopted in the production of bacterial classification breeding, and the mode that hockets to accelerate the reproduction speed of bacterial classification, improves the viability of bacterial classification.
2, the mode of production of liquid bacterium gill fungus also adopts " soilless culture " similar in appearance to plant husbandry, uses the skeleton material of nontoxic, water insoluble and weak acid, weak base, as the quartz sand of cleaning.The raw material of medium all is nontoxic, edible, Organic Ingredients such as soybean, corn, rice, wheat bran, yeast, etc.; Inorganic elements such as calcium, magnesium, sylvite etc.; And some vitamin.Concrete prescription can be regulated because of the bacterium gill fungus kind difference of cultivating.
3, the characteristics of liquid bacterium gill fungus production technology are fully to analyze, studying on the pluses and minuses and in-problem basis of solid culture and two technology of submerged fermentation, the new technology of research and development.It is to be carrier with solid culture medium or skeleton material (no nutritive value and no DDGS), make the liquid culture material constantly or intermittent flow cross solid culture medium or skeleton material, thereby having solved solid culture can not regulate and control the moisture of fermentation, nutrition supply, acid-base value etc. fermentation this critical period people, though and the air capacity of soils of medium is good, oxygen supply is the contradiction of difficulty still; Also solve simultaneously deep layer and cultivated a series of problems of bringing because of outstanding oxygen supply contradiction.
4, the new technology of liquid bacterium gill fungus production is not only applicable to the production of liquid bacterium gill fungus, is applicable to solid fermentation yet, and the large-scale production of bacterial classification, equally is applicable to industrial fermentation, medicine fermentation yet.
5, the liquid bacterium gill fungus fermentation equipment important component part that is liquid bacterium gill fungus production method.The exploitation of microorganism, utilization, industrialization are new industries, and the characteristics of production technology and key are fermentation techniques, belong to the fermentation engineering of contemporary bioscience four big cutting edge technologies.Equipment of the present invention has solved the production problem of liquid bacterium gill fungus, particularly the oxygen supply difficult problem of aerobic solid, liquid body fermentation.
Description of drawings
Fig. 1 is the primary structure schematic diagram of liquid bacterium gill fungus fermentation equipment.
Embodiment
Liquid bacterium gill fungus production method of the present invention comprises culture propagation, connects bacterial classification, fermentation in medium, and connecing bacterial classification and fermentation in medium is not carry out in liquid bacterium gill fungus fermentation is not equipped with, and the steps include:
In the porous concentrator bowl, put solid culture medium or skeleton material, tapping body medium or total nutrient liquid medium in liquid reserve tank.Solid culture medium and liquid nutrient medium are used, and the skeleton material is used with full nutrient medium.
1) puts into solid culture medium or the skeleton material medium that process is sterilized in the porous concentrator bowl in liquid bacterium gill fungus fermentation equipment machine, insert the bacterial classification uniform mixing, bacterial classification accounts for 8%~20% (weight) of medium, constantly adds oxygen by the air admission hole on the body, ferments 2~3 days;
2) in the liquid reserve tank on the liquid bacterium gill fungus fermentation equipment liquid nutrient medium (or total nutrient liquid medium) is housed, by the porous woven hose liquid nutrient medium (or total nutrient liquid medium) is joined in the solid culture medium (or skeleton material medium) in the porous concentrator bowl, actuating motor drives the rotation of porous concentrator bowl simultaneously, the effect of stirring is also played in the rotation of porous concentrator bowl, make liquid nutrient medium pass solid culture medium (or skeleton material medium), supplement the nutrients and oxygen, and the adjusting of acid-base value, by centrifugal force excess liquid culture fluid and fermentation product are taken in the outer body of concentrator bowl simultaneously; (or the total nutrient liquid medium is 4~5 times of solid culture medium (or skeleton material medium) to the liquid nutrient medium that adds;
3) per 0.5~1.0 hour, the liquid medium in the body and fermentation product pump to liquid reserve tank, are joined in the porous concentrator bowl by the porous woven hose, actuating motor drives the rotation of porous concentrator bowl once simultaneously, circulates repeatedly; Cultivate again and promptly finished in 2~3 days;
4) the liquid-solid mixed culture fermentation product in the body is emitted from the tap hole of underpart.
Solid culture medium is made up of agricultural product particle, wheat bran, dusty yeast etc., and the skeleton material is the granular materials of nontoxic, water insoluble, weak acid, weak base.
Liquid nutrient medium is the water-soluble solution of mineral salt, sugar etc., and the complete nutrition medium then also comprises organic carbon source, nitrogenous source, vitamin etc.
Fig. 1 is the primary structure schematic diagram of liquid bacterium gill fungus fermentation equipment.
Liquid bacterium gill fungus fermentation equipment comprises charging aperture 1-1 on body 1, the body and the discharging opening 1-2 under the body, be provided with porous concentrator bowl 2 in the body, porous second woven hose 3 is arranged in the porous concentrator bowl 2, the top of porous woven hose 3 is passed body upper and is communicated with liquid reserve tank 6, body upper has air admission hole 7, venthole 14, axle 4 below the porous concentrator bowl 2 passes organism bottom and is connected with driving mechanism, and driving mechanism comprises pulley transmission mechanism 5 and drive motors 15.Have loop exit 10 to be connected with infusion pump 11 under the body, infusion pump 11 is connected with liquid reserve tank 6 or porous woven hose 3 by pipeline 12.Air admission hole 7 can be connected with air pump 8, air cleaner 9.The course of work:
1, the breeding of bacterial classification: bacterial classification comprises edible and medicinal bacterial classification.
A, first kind of mode: test tube strains-→ the granule bacterial classification-→ liquid spawn.
B, the second way: with in the test tube or the healthy and strong mushroom flower bud of the assorted bacterium of the nothing of growing in the seed bottle, under aseptic condition, pulverize, be inoculated in the sterile granules medium, stir, under the temperature condition about 20 ℃, cultivate, after mycelia is covered with, checking does not have assorted bacterium, stalwartness, can use.
2, the production of liquid bacterium gill fungus: in liquid bacterium gill fungus fermentation equipment, carry out.
A, first kind of mode:
Put into the solid culture medium (or skeleton material medium) through sterilization in concentrator bowl, the prescription of medium is different different because of liquid bacterium gill fungus kind, the product requirement produced.As: glossy ganoderma then adds 20% wheat bran again with the corn particle, or adds an amount of dusty yeast with soya bean particle or corn particle.Chinese caterpillar fungus is used the rice particle, etc.The water content of medium is 80~100%, and is lower than general solid culture medium.Acid-base value is different and difference to some extent is generally nature with the kind of bacterium gill fungus, and is promptly neutral.
The prescription of liquid nutrient medium or total nutrient liquid medium is also different different because of liquid bacterium gill fungus kind, the product requirement produced.As: glossy ganoderma is a potassium dihydrogen phosphate 0.1~0.3%, magnesium sulfate 0.05~0.01%, sugar 1~2% is put into fluid storage tank after sterilization, standby.
Bacterial classification generally is connected in the solid culture medium 13, all can with solid spawn or liquid spawn.Inoculum concentration is about 8~20%, and tries one's best and the medium uniform mixing.
After the inoculation, carry out cultivation and fermentation under the temperature about 20 ℃, open air pump 8, air filters through air cleaner 9, is sent into by air admission hole 7 and supplies fermentation usefulness in the body 1, and sending out a bacterium incubation time is 2~3 days.After mycelium covers with substantially, begin to import liquid nutrient medium, actuating motor 15, pulley transmission mechanism 5 drive concentrator bowl 2 rotations, and stirring action is played in concentrator bowl 2 rotations, makes liquid nutrient medium pass solid culture medium, ample supply nutrients and oxygen; Simultaneously, by centrifugal force excess liquid culture fluid, fermentation product are taken in body 1 lower floor.
Per 0.5~1.0 hour, start pump 11 with the liquid medium in the body and fermentation product pump to liquid reserve tank 6, join again in the porous concentrator bowl 2 by porous woven hose 3, actuating motor drives the rotation of porous concentrator bowl once simultaneously, circulates repeatedly; Cultivate again and promptly finished in 2~3 days.
Fermentation product in the body is emitted from the tap hole of underpart.
The water content of regulating solid culture medium by the rotating speed of adjusting concentrator bowl 2.Rotary speed difference, centrifugal force size are also just different, and the water content of solid culture medium is also had any different.
The second way:
Without solid culture medium, and with full nutrient medium of liquid and skeleton material, this mode is similar to " soilless culture " of plant husbandry.
A, bacterial classification all can with liquid strain or solid particle kind.
B, inoculum concentration are about 10%, directly are inoculated in the liquid nutrient medium.
Put the skeleton material in c, the concentrator bowl, the skeleton material is the granular pattern skeleton material of nontoxic, water insoluble, weak acid, weak base, as quartz sand.The particle of quartz sand is greater than the hole of porous concentrator bowl 2 sidewalls.
The prescription of d, total nutrient liquid medium is also different different because of liquid bacterium gill fungus kind, the product requirement produced.As: glossy ganoderma can be the soya-bean milk that contains soya bean 2~3%, sugar 1%, peptone 0.1%, VB110 micrograms per litre, dusty yeast 0.2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%.The acid-base value nature.
About 15~25 ℃ of e, fermentation temperatures.
F, per 0.5~1.0 hour circulation primary of liquid nutrient medium.
G, incubation time are about 3 days.
The second way only is that with the difference of first kind of mode perfect medium is different, and other technology is identical.
Core of the present invention is to be carrier with solid culture medium or skeleton material (no nutritive value and no DDGS); make liquid nutrient medium constantly or intermittent flow cross solid culture medium or skeleton material; thereby solved in this critical period of fermentation the moisture, nutrition supply, acid-base value etc. of fermentation are regulated and control and the oxygen supply problem; therefore; every make the liquid culture material constantly or intermittent flow cross solid culture medium or skeleton material and realize fermentation moisture, nutrition supply, acid-base value and oxygen supply etc. are regulated and control, all belong to protection scope of the present invention.

Claims (5)

1. a liquid bacterium gill fungus production method comprises culture propagation, connects bacterial classification, fermentation in medium, it is characterized in that: connecing bacterial classification and fermentation in medium is to carry out in liquid bacterium gill fungus fermentation equipment, the steps include:
1) puts into the solid culture medium of process sterilization or the skeleton material medium that the skeleton material mixes with the total nutrient liquid medium in the porous concentrator bowl in liquid bacterium gill fungus fermentation equipment body, with the bacterial classification uniform mixing, bacterial classification accounts for 8%~20% of medium, constantly add oxygen by the air admission hole on the body, fermented 2~3 days;
2) in the liquid reserve tank on the liquid bacterium gill fungus fermentation equipment liquid nutrient medium or total nutrient liquid medium are housed, by the porous woven hose liquid nutrient medium or total nutrient liquid medium are joined in the solid culture medium or skeleton material medium in the porous concentrator bowl, actuating motor drives the rotation of porous concentrator bowl simultaneously, the effect of stirring is played in the rotation of porous concentrator bowl, make liquid nutrient medium or total nutrient liquid medium pass solid culture medium or skeleton material medium, extra-nutrition thing and oxygen take excess liquid culture fluid and fermentation product in the outer body of concentrator bowl to by centrifugal force simultaneously; Liquid nutrient medium that adds or total nutrient liquid medium are solid culture medium or skeleton material medium 4~5 times;
3) per 0.5~1.0 hour, the liquid medium in the body and fermentation product pump to liquid reserve tank, are joined in the porous concentrator bowl by the porous woven hose again, actuating motor drives the rotation of porous concentrator bowl once simultaneously, circulates repeatedly; Cultivate again and promptly finished in 2~3 days;
4) the liquid-solid mixed culture fermentation product in the body is emitted from the tap hole of underpart.
2. liquid bacterium gill fungus production method according to claim 1, it is characterized in that: solid culture medium comprises agricultural product particle, wheat bran, dusty yeast and traditional Chinese medicine.
3. liquid bacterium gill fungus production method according to claim 1 is characterized in that: the skeleton material is the graininess skeleton material of nontoxic, water-fast particle greater than the hole of porous concentrator bowl sidewall; The total nutrient liquid medium comprises slurry, sugar, mineral salt, organic carbon, organic nitrogen, the enzyme of crops.
4. liquid bacterium gill fungus production method according to claim 1, it is characterized in that: liquid nutrient medium comprises slurry, sugar, the mineral salt of crops.
5. liquid bacterium gill fungus fermentation equipment that is used for the arbitrary liquid bacterium gill fungus production method of claim 1~4, comprise charging aperture on body, the body and the discharging opening under the body, it is characterized in that: be provided with the porous concentrator bowl in the body, the built-in porous woven hose of porous concentrator bowl, the top of porous woven hose is passed body upper and is communicated with liquid reserve tank, body upper has air admission hole and air vent, axle below the porous concentrator bowl passes organism bottom and is connected with driving mechanism, underpart has loop exit to be connected with infusion pump, and infusion pump is connected with liquid reserve tank or porous woven hose by pipeline.
CNB2006100183991A 2006-02-23 2006-02-23 Liquid mushroom producing method and fermenting set Expired - Fee Related CN100513549C (en)

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Application Number Priority Date Filing Date Title
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107746805A (en) * 2017-11-17 2018-03-02 江南大学 A kind of method of Liquid static culture edible and medicinal fungi
CN109370886A (en) * 2014-12-17 2019-02-22 宁夏伊品生物科技股份有限公司 Fed-batch cultivation equipment
CN109370882A (en) * 2014-12-17 2019-02-22 宁夏伊品生物科技股份有限公司 Equipment used in the fermentation of lysine and component
CN109892168A (en) * 2019-03-28 2019-06-18 许晴 Amphibious fermentation method

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN85103034A (en) * 1985-04-16 1986-10-15 广西灵川县食用菌种厂 Xianggu mashroom open cultivate technique by bagasse mixed with liquid fungus species
CN1008319B (en) * 1985-11-02 1990-06-13 赖南贤 Method to culture edible fungi products by combining solid with liquid media
CN86102421B (en) * 1986-04-10 1988-11-09 余兆丰 Liquid culture method of edible fungi

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109370886A (en) * 2014-12-17 2019-02-22 宁夏伊品生物科技股份有限公司 Fed-batch cultivation equipment
CN109370882A (en) * 2014-12-17 2019-02-22 宁夏伊品生物科技股份有限公司 Equipment used in the fermentation of lysine and component
CN109370882B (en) * 2014-12-17 2021-08-20 宁夏伊品生物科技股份有限公司 Apparatus and components for use in fermentation of lysine
CN109370886B (en) * 2014-12-17 2021-08-31 宁夏伊品生物科技股份有限公司 Feeding fermentation equipment
CN107746805A (en) * 2017-11-17 2018-03-02 江南大学 A kind of method of Liquid static culture edible and medicinal fungi
CN107746805B (en) * 2017-11-17 2019-04-23 江南大学 A kind of method of Liquid static culture edible and medicinal fungi
CN109892168A (en) * 2019-03-28 2019-06-18 许晴 Amphibious fermentation method

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