CN110052301B - Motion micro-droplet continuous separation method based on y-cut lithium niobate sandwich structure chip - Google Patents

Motion micro-droplet continuous separation method based on y-cut lithium niobate sandwich structure chip Download PDF

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CN110052301B
CN110052301B CN201910427611.7A CN201910427611A CN110052301B CN 110052301 B CN110052301 B CN 110052301B CN 201910427611 A CN201910427611 A CN 201910427611A CN 110052301 B CN110052301 B CN 110052301B
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droplets
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lithium niobate
sandwich structure
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CN110052301A (en
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阎文博
张�雄
高作轩
高开放
陈洪建
昝知韬
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Hebei University of Technology
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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Abstract

The invention discloses a method for continuously separating moving micro-droplets based on a y-cut lithium niobate sandwich structure chip, which takes a sandwich structure consisting of y-cut lithium niobate wafers as a core, utilizes a superposed electric field formed by laser intermittent irradiation to realize the continuous separation of the micro-droplets in a sandwich layer, solves the problem that the micro-droplets are difficult to continuously separate after polarization, and can realize the separation of the micro-droplets with different sizes through the laser power and the intermittent irradiation distance. The technology can be applied to the transportation and separation of trace reagents, and has important significance for the development of the fields of biological medicine, medical diagnosis, environmental monitoring, molecular biology and the like.

Description

Motion micro-droplet continuous separation method based on y-cut lithium niobate sandwich structure chip
Technical Field
The invention relates to a micro-droplet control technology, in particular to a moving micro-droplet continuous separation method based on a y-cut lithium niobate sandwich structure chip
Background
With the rapid development of microfluidic chips, micro-droplet manipulation has become a research hotspot in the field. The micro-droplet control technology is mainly applied to analysis and detection of trace samples in the processes of biology, chemistry, pharmacy and the like, and mainly relates to collection, mixing, transportation and the like of trace reagents. It has very important significance for the development of the fields of biological medicine, medical diagnosis, food sanitation, environmental monitoring, molecular biology and the like.
Esseling et al (Optofluidic droplet router [ J ] Laser and Photonics Reviews, 2015, 9 (1): 98-104) reported a method of dielectric droplet transport and separation using dielectrophoretic forces, called a micro-droplet router. The router mainly utilizes a mask plate to realize spatial modulation of laser, and controls micro-droplets by using a photovoltaic electric field generated by laser-induced c-cut lithium niobate. They have proposed in the article that the direction of dielectrophoresis can be determined by comparing the magnitude of the dielectric constant of the manipulated droplet and the medium. When the dielectric constant of the controlled liquid drop is larger than that of the surrounding medium, the direction of the controlled liquid drop subjected to dielectrophoresis points to the illumination position, and the liquid drop is attracted to move along the illumination position; when the dielectric constant of the controlled liquid drop is smaller than that of the surrounding medium, the direction of the dielectrophoresis force applied to the controlled liquid drop is opposite to the illumination direction, and the illumination strip-shaped pattern is like a virtual barrier and prevents the liquid drop from moving towards the illumination position. And in the experiment, the micro-droplet splitting is successfully realized by using two strip-shaped illumination patterns as virtual attraction light paths.
Gazzetto M et al (Numerical and Experimental Study of Optoelectronic trading Iron-Doped Lithium Niobate Substrate [ J ]. Crystals, 2016, 6 (10): 123.) in 2016 successfully achieved the capture of water droplets dispersed in oil on y-cut Lithium Niobate Crystals by dielectrophoretic forces. They dispersed polymethyl methacrylate (PMMA) fine particles in an oil film, and uniformly dropped the same onto a lithium niobate crystal, and after irradiating for 30 seconds with a laser of 532nm, the trapping of the fine particles was observed both at the upper and bottom of the laser irradiation region. By the same experimental procedure, the presence of dielectrophoretic forces, i.e. attraction or repulsion phenomena on the particles, was not observed for the particles immersed in the aqueous solution. However, they observed the presence of positive dielectrophoretic forces by dispersing 2-10 um water droplets into the oil, achieving attraction of the water droplets.
Chu Y et al (Remote management of a micro nanoparticle in Water by Near-Infrared Laser, 8, 1273-3The remote control of the micro-droplets can ensure that the droplets can ascend, shuttle, horizontally move and even suspend in water under the action of laser. Although the method can make the micro-droplets reciprocate along any one-dimensional path, the packaging process of the nano-particles is complexThe micro liquid drops can be polluted, the internal environment of the liquid drops can be disturbed by long-time irradiation of the liquid drops by laser, and the method can only be applied to the micro liquid drops packaged in the water environment, so that the application range of the method is greatly limited.
Disclosure of Invention
The invention provides a simple and feasible method for continuously separating moving micro-droplets, which can realize continuous separation of the micro-droplets in the transportation process, does not need any pretreatment on the droplets, is not influenced by the polarization of the droplets, and is controllable in real time in the whole process.
A continuous separation method of moving micro-droplets is characterized in that: and the y-cut lithium niobate sandwich structure chip which is reversely configured in the c-axis direction is used as a substrate, and in the process of driving the micro-droplets to move by laser, the micro-droplets are continuously separated along the c-axis direction in the moving process by using the discontinuous irradiation of the laser.
A continuous separation method of moving micro-droplets is characterized in that: the separation of the liquid drops is realized by utilizing the dielectrophoresis force generated by the superimposed heterogeneous electric field formed by the lithium niobate chips before and after the intermittent irradiation of the laser on the liquid drops.
A continuous separation method of moving micro-droplets is characterized in that: continuous separation of micro-droplets with different sizes can be realized by controlling the laser power and the intermittent irradiation distance.
Compared with the prior art, the invention has the advantages that: the continuous separation of the micro-droplets can be realized in the transportation process, and the problem that the micro-droplets are difficult to separate after polarization is solved; the micro liquid drops with different sizes can be separated by the laser power and the intermittent irradiation distance.
Drawings
FIG. 1 is a schematic structural diagram of a device adopted by the method for continuously separating moving micro-droplets based on a y-cut lithium niobate sandwich structure chip.
Fig. 2 is a schematic diagram of a specific structure of a lithium niobate sandwich structure chip based on a y-cut lithium niobate sandwich structure chip motion micro-droplet continuous separation method of the present invention.
FIG. 3 is a schematic diagram of the principle of the method for continuously separating moving micro-droplets based on a y-cut lithium niobate sandwich chip.
Fig. 4 is a diagram of a moving droplet continuous separation process of an embodiment (example 1) of the moving micro droplet continuous separation method based on the y-cut lithium niobate sandwich structure chip of the present invention.
Fig. 5 is a diagram of a moving droplet continuous separation process of an embodiment (example 2) of the moving micro droplet continuous separation method based on the y-cut lithium niobate sandwich structure chip of the present invention.
Fig. 6 is a moving droplet continuous separation process diagram of an embodiment (example 3) of the moving micro droplet continuous separation method based on the y-cut lithium niobate sandwich structure chip of the present invention.
Detailed Description
The invention will be further illustrated by the following examples and figures
The invention discloses a method for continuously separating moving liquid drops, which comprises the following steps: the laser 1, the electronic shutter 2, the diaphragm 3, the laser reflector 6, the objective lens 7, the lithium niobate sandwich structure chip 8 and the transparent objective table 9 form a moving micro-droplet separation optical path in sequence; a background light source 10, a transparent objective table 9, a lithium niobate sandwich structure chip 8, an objective lens 7, a laser reflector 6, an optical filter 5 and a CCD camera 4 form a real-time observation light path in sequence; the servo motor 11 and the transparent stage 9 form a moving means in this order. All optical devices and electronic devices are fixed on the optical experiment platform through rigid connecting rods, and coaxial alignment of all elements is guaranteed.
The invention discloses a continuous separation method of moving liquid drops, which comprises the following operation steps: introducing a liquid 12 to be manipulated into a lithium niobate sandwich structure chip (consisting of two y-cut lithium niobate wafers, namely 8-1 and 8-2), placing the lithium niobate sandwich structure chip 8 on a transparent objective table 9, controlling the position of the transparent objective table 9 by adjusting a servo motor 11, enabling the liquid 12 to be manipulated to be positioned near the focus of an objective lens 7, and capturing a clear image by using a CCD camera 4; the laser 1 is opened, the laser power is adjusted to be a proper value, the position of the interlayer chip is adjusted through the servo motor 11, the liquid to be controlled is positioned at the irradiation position of the laser 13, the electronic shutter 2 is opened, meanwhile, the interlayer chip is moved through the servo motor, the liquid drops move along with the laser along the direction of the c axis, the effect of intermittent irradiation of the laser is achieved by closing the electronic shutter for a period of time, the movement state of the interlayer chip is kept unchanged during the intermittent irradiation, and the continuous separation of the moving micro-liquid drops is achieved through reasonable matching of the laser power and the intermittent irradiation distance.
The laser 1 is required to emit laser to irradiate the lithium niobate wafer to effectively excite carriers, and a formed space electric field can reach the magnitude of manipulating dielectric liquid drops, so that the wavelength of the laser is 350-550 nm, and the power of the laser is 3-110 mW; the background light source 10 may use a xenon lamp, a halogen lamp, or a high-power white LED lamp; the magnification of the objective lens 7 is 4 to 25 times.
The working principle of the scheme of the invention is as follows: when the laser irradiates the surface of the y-cut lithium niobate wafer, directionally moving light-excited carriers (electrons) can be generated, the light-excited carriers move along the positive direction of the c axis of the lithium niobate wafer, two positive and negative charge areas (the positive direction of the c axis is a negative charge accumulation area, and the negative direction of the c axis is a positive charge accumulation area) distributed along the c axis can be formed on the y-cut lithium niobate wafer irradiated by the laser, an electric field formed by the positive and negative charges generates dielectrophoresis force on micro droplets, when the laser moves along the c axis direction relative to the y-cut lithium niobate wafer, the charge areas can be overlapped (the positive and negative charges are neutralized), when the laser is discontinuously irradiated, the positive charge area before the discontinuous irradiation is overlapped with the negative charge area after the discontinuous irradiation, two positive and negative charge areas with the distance larger than the radius of the laser are formed, and the charges in the two areas are reduced due to neutralization and even close to zero, the dielectrophoresis force generated by the charge distribution on the micro droplets is very favorable for separating the droplets, the micro-droplets can be separated in the moving process.
Specific examples of the scheme for realizing the continuous separation of moving droplets according to the present invention are given below, and the specific examples are only used for illustrating the present invention in detail and do not limit the protection scope of the claims of the present application.
Example 1
The method comprises the steps of using a 405nm laser, enabling the laser power to be 3.8mW, enabling a background light source to be a halogen lamp, enabling the magnification of a focusing objective lens to be 16 times, introducing liquid to be manipulated into a lithium niobate sandwich structure chip, adjusting the position of the chip through a servo motor, enabling a micro-droplet to be manipulated to be 60 microns, opening an electronic shutter, controlling the droplet to move along with the laser, closing the electronic shutter, enabling the motion state of the chip to be unchanged during the closing period of the electronic shutter, enabling the displacement of the chip subjected to intermittent irradiation to be 20 microns, and opening the shutter again to achieve continuous separation of the micro-droplet.
Example 2
The method comprises the steps of using a 405nm laser, enabling the laser power to be 4.3mW, enabling a background light source to be a halogen lamp, enabling the magnification of a focusing objective lens to be 16 times, introducing liquid to be operated into a lithium niobate sandwich structure chip, adjusting the position of the chip through a servo motor, enabling a micro-droplet to be operated to be 80 microns, opening an electronic shutter, controlling the droplet to move along with the laser, closing the electronic shutter, enabling the motion state of the chip to be unchanged during the closing period of the electronic shutter, enabling the displacement of the chip subjected to intermittent irradiation to be 35 microns, and opening the shutter again to achieve continuous separation of the micro-droplet.
Example 3
The method comprises the steps of using a 405nm laser, enabling the laser power to be 8mW, enabling a background light source to be a halogen lamp, enabling the magnification of a focusing objective lens to be 16 times, introducing liquid to be manipulated into a lithium niobate sandwich structure chip, adjusting the position of the chip through a servo motor, enabling an electronic shutter to be opened, controlling the liquid drop to move along with the laser, closing the electronic shutter, enabling the movement state of the chip to be unchanged during the closing period of the electronic shutter, enabling the displacement of the chip subjected to intermittent irradiation to be 60 microns, and opening the shutter again to achieve continuous separation of the liquid drops.
The above embodiments are further described in detail, it should be understood that the above embodiments are not intended to limit the present invention, and all equivalent modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be considered within the scope of the present invention.

Claims (3)

1. A motion micro-droplet continuous separation method based on a y-cut lithium niobate sandwich structure chip is characterized in that: the y-cut lithium niobate sandwich structure chip which is reversely configured in the c-axis direction is used as a substrate, in the process of driving the micro-droplets to move by laser, the micro-droplets are continuously separated along the c-axis direction in the moving process by using the discontinuous irradiation of the laser, the laser wavelength is 350-550 nm, and the power is 3-110 mW.
2. A method for the continuous separation of moving micro-droplets according to claim 1, characterized in that: the separation of the liquid drops is realized by utilizing the dielectrophoresis force generated by the superimposed heterogeneous electric field formed by the lithium niobate chips before and after the intermittent irradiation of the laser on the liquid drops.
3. The method for continuously separating moving micro-droplets as claimed in claim 1, wherein the continuous separation of micro-droplets with different sizes can be realized by controlling the laser power and the intermittent irradiation distance.
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CN110560189B (en) * 2019-10-21 2022-02-01 河北工业大学 Hydrated liquid drop separation method and device based on y-cut lithium niobate chip
CN114425462B (en) * 2020-10-29 2023-10-31 京东方科技集团股份有限公司 Microfluidic chip and preparation method thereof
CN114225977A (en) * 2021-11-25 2022-03-25 西安电子科技大学 Multi-core multi-component micro-droplet processing system

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