CN110050757B - Method for testing eating preference of earthworms - Google Patents
Method for testing eating preference of earthworms Download PDFInfo
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- CN110050757B CN110050757B CN201910287400.8A CN201910287400A CN110050757B CN 110050757 B CN110050757 B CN 110050757B CN 201910287400 A CN201910287400 A CN 201910287400A CN 110050757 B CN110050757 B CN 110050757B
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- 241001233061 earthworms Species 0.000 title claims abstract description 90
- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000012360 testing method Methods 0.000 title claims abstract description 24
- 229920001817 Agar Polymers 0.000 claims abstract description 44
- 239000008272 agar Substances 0.000 claims abstract description 44
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 241000361919 Metaphire sieboldi Species 0.000 claims abstract description 17
- 238000001816 cooling Methods 0.000 claims abstract description 15
- 238000004519 manufacturing process Methods 0.000 claims abstract description 3
- 239000011521 glass Substances 0.000 claims description 89
- 238000005192 partition Methods 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000000049 pigment Substances 0.000 claims description 5
- 229920003023 plastic Polymers 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 229920005372 Plexiglas® Polymers 0.000 claims 2
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 claims 1
- 239000004926 polymethyl methacrylate Substances 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 8
- 239000003814 drug Substances 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 238000000855 fermentation Methods 0.000 description 10
- 230000004151 fermentation Effects 0.000 description 10
- 239000002994 raw material Substances 0.000 description 6
- 241000609240 Ambelania acida Species 0.000 description 5
- 241000233866 Fungi Species 0.000 description 5
- 239000010905 bagasse Substances 0.000 description 5
- 239000010871 livestock manure Substances 0.000 description 3
- 238000010422 painting Methods 0.000 description 3
- 238000005498 polishing Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 230000035611 feeding Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0332—Earthworms
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to the technical field of agricultural ecology, in particular to a method for testing eating preference of earthworms, which can conveniently test eating preference of earthworms, thereby facilitating symptomatic medicine administration and ensuring survival amount of earthworms in soil; the method comprises the following steps: (1) manufacturing a feeding tube; (2) filling feed; (3) testing the feeding preference of earthworms: selecting a healthy earthworm, putting the earthworm into a feeding pipe, and enabling the earthworm to continuously move along the feeding pipe and take food; (4) baking treatment; (5) preparing agar liquid; (6) fixing a food taking channel: pouring the agar liquid into a feeding pipe from the other end of the feeding pipe, cooling, and after the agar liquid is completely solidified, fixing the shape of the earthworm feeding channel; (7) cutting a sample: slowly pushing the fixed part in the feeding tube out of the feeding tube, and cutting off the part along the tube opening of the feeding tube by using an art knife to obtain a plurality of samples; (8) quantitative recording of feeding preferences: the relative feeding preference of the earthworms is calculated according to the percentage.
Description
Technical Field
The invention relates to the technical field of agricultural ecology, in particular to a method for testing eating preference of earthworms.
Background
Earthworms form numerous reaction circles inside and outside the body through activities such as feeding, digestion, excretion, digging and the like, thereby influencing the biological, chemical and physical processes of an ecosystem, the earthworms are used as 'scavenger' for purifying soil, are 'engineers' of the soil ecosystem, are consumers, decomposers and regulators in the ecosystem, and have the functions in the ecosystem specifically shown as follows: a) influence on the processes of organic matter decomposition, nutrient circulation and the like in soil; b) influence on the physicochemical properties of soil; c) interaction with plants, microorganisms, and other animals. At present, however, in a farmland in which fertilizers and pesticides are used for a long time, the number of earthworms is reduced in a large proportion and even disappears, and the phenomenon not only causes the soil fertility to be rapidly reduced, but also causes the soil-borne diseases to be popular day by day, so that the problem that how to measure the eating preference of the earthworms is to be solved is a problem which needs to be solved urgently, and the research aspect of the eating preference of the earthworms in our country is lack of a specific test method and a quantitative index.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for testing the feeding preference of earthworms, which can conveniently determine the feeding preference of the earthworms, thereby conveniently administering medicines according to symptoms and ensuring the survival amount of the earthworms in soil.
The invention discloses a method for testing eating preference of earthworms, which comprises the following steps:
(1) manufacturing a feeding tube: taking an 80cm organic glass tube, dividing the space in the organic glass tube into two equal parts by using a partition plate, and plugging one end of the organic glass tube by using gauze;
(2) filling feed: filling different feeds A and B into two parts formed in an organic glass tube respectively, slightly and forcibly extruding the filled feeds respectively, after filling, drawing out the partition plate, and vertically placing the organic glass tube for later use to obtain a complete feeding tube;
(3) testing the feeding preference of earthworms: selecting a healthy earthworm, putting the earthworm into a feeding pipe filled with two different feeds, and plugging the pipe head of the feeding pipe by a plastic plug with a pinhole-sized vent hole, so that the earthworm continuously moves to one side of a gauze plug along the feeding pipe and takes the earthworm;
(4) baking treatment: when the earthworms take food along the feeding pipe and move to the other side of the pipe, the earthworms are taken out, and the feeding pipe is placed into an oven for baking treatment, so that the temperature in the feeding pipe is uniform;
(5) preparing an agar solution: heating 1000ml of water to 60 ℃, adding 20g of agar into the water, and stirring the agar and the agar to uniformly mix the agar and the agar to obtain agar liquid;
(6) fixing a food taking channel: plugging one end of a feeding pipe, erecting the feeding pipe, pouring the agar liquid into the feeding pipe from the other end of the feeding pipe, filling holes drilled in the feeding pipe by earthworms from top to bottom with the agar liquid, filling gaps between two different feeds and gaps between the two different feeds and the inner wall of an organic glass pipe, standing, cooling to room temperature, and finishing the fixation of the shape of an earthworm feeding channel after the agar liquid is completely solidified;
(7) cutting a sample: slowly pushing the fixed part in the feeding tube out of the feeding tube, and cutting off the part along the tube opening of the feeding tube by using an art designer knife when pushing out the part to obtain a plurality of samples;
(8) quantitative recording of feeding preferences: the eating preference of the earthworms tested by a plurality of samples is recorded as feed A or feed B, and the relative eating preference of the earthworms is calculated according to percentage.
The invention discloses a method for testing eating preference of earthworms, which comprises the following steps of (1) dividing the space in an organic glass tube into two equal parts by using a partition plate:
a. marking two dividing lines along the direction of the organic glass tube at symmetrical positions of the inner wall of the organic glass tube;
b. two parting lines on the inner wall of the organic glass tube are clearly coated by bright pigments;
c. taking an organic glass plate, and cutting out an organic glass partition plate which is 85cm long and has the same width as the inner diameter of the organic glass pipe;
d. the edge of the organic glass partition plate is polished and repaired, and the organic glass partition plate is inserted into the organic glass tube along two partition lines in the organic glass tube, so that the organic glass tube is divided into two equally-divided semi-cylindrical spaces.
The invention relates to a method for testing eating preference of earthworms, wherein the diameter of an organic glass tube in the step (1) is 1 cm-2 cm.
According to the method for testing the feeding preference of the earthworms, the depth of the feed filling in the step (2) is 60cm +/-2 cm.
According to the method for testing the feeding preference of the earthworms, the diameter of the annulus of the earthworms selected in the step (3) is 1/2 of the inner diameter of the organic glass tube.
According to the method for testing the eating preference of the earthworms, the baking treatment temperature in the step (4) is 55 +/-2 ℃, and the baking treatment time is 60 min.
According to the method for testing the eating preference of the earthworms, the cooling mode to the room temperature in the step (6) is natural cooling or air cooling.
According to the method for testing the feeding preference of the earthworms, the cutting thickness of the sample in the step (7) is 2 cm.
Compared with the prior art, the invention has the beneficial effects that:
1. the earthworms are allowed to do directional feeding movement in a limited space;
2. selecting earthworms with proper size to match with the pipe diameter, so that the earthworms cannot turn around in the pipe;
3. different feeds are subpackaged by partition plates in equal proportion, so that earthworms can continuously and freely select to observe the feeding characteristics of the earthworms in the feeding process, and the conversation between people and the earthworms is realized;
4. fixing the feeding cave of the earthworms by using agar as a fixing agent;
5. the cave positions left after earthworms eat are recorded by a sectional recording method, so that the relative eating preference of the earthworms is quantified;
the relative feeding preferences of earthworms were tested by the above method and the criteria for such feeding preferences were quantified. Provides technical support and quantitative standards for the basic research of earthworms. Provides technical support for the research work of soil ecological balance represented by earthworms.
Detailed Description
The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1
The livestock manure and the waste straw are used as raw materials for fermentation, and the raw materials are divided into two parts in the fermentation process, wherein one part is added with partial bagasse in the fermentation process, so that feed A without bagasse and feed B with bagasse are obtained.
Taking an 80cm organic glass tube with the diameter of 1cm, marking two dividing lines at symmetrical positions on the inner wall of the organic glass tube along the direction of the organic glass tube, clearly painting the two dividing lines on the inner wall of the organic glass tube by using bright pigments, taking an organic glass plate, cutting an organic glass partition plate which is 85cm long and has the same width as the inner diameter of the organic glass tube, polishing and repairing the edge of the organic glass partition plate, inserting the organic glass partition plate into the organic glass tube along the two dividing lines in the organic glass tube, dividing the organic glass tube into two equally-divided semi-cylindrical spaces, plugging one end of the organic glass tube by using gauze, respectively filling different feeds A and B into two parts formed in the organic glass tube, respectively extruding the filled feeds slightly, after filling 60cm of feeds, drawing out the partition plate, and vertically placing the organic glass tube for later use, obtaining a complete feeding tube;
selecting 1/2 healthy earthworms with the diameter of the organic glass tube at the position of an annulus, putting the earthworms into a feeding tube filled with two different feeds, plugging the tube head of the feeding tube by a plastic plug with a pinhole-sized vent hole, enabling the earthworms to continuously move to one side of a gauze plug along the feeding tube and take the earthworms, taking the earthworms out when the earthworms take the earthworms and move to the other side of the tube along the feeding tube, putting the feeding tube into an oven, and baking for 60min at the temperature of 55 ℃ to enable the temperature in the feeding tube to be uniform;
heating 1000ml of water to 60 ℃, adding 20g of agar into the water, stirring the mixture to uniformly mix the agar and the agar to obtain an agar liquid, plugging one end of a feeding pipe, erecting the feeding pipe, pouring the agar liquid into the feeding pipe from the other end of the feeding pipe, filling holes drilled in the feeding pipe by earthworms from top to bottom with the agar liquid, filling gaps between two different feeds and gaps between the two different feeds and the inner wall of an organic glass pipe, standing, naturally cooling or air cooling to room temperature, completing the fixation of the earthworm feeding channel shape after the agar liquid is completely solidified, slowly pushing the fixed part in the feeding pipe out of the feeding pipe, cutting the pipe along the feeding pipe by using a utility knife every 2cm to obtain samples at 30 positions, recording whether the feeding preference of the earthworms tested by the samples at multiple positions is feed A or feed B, and calculating the relative feeding preference of the earthworms according to the feeding percentage, selecting a plurality of different earthworms to repeat the operation.
Wherein the proportion of the preference feed A is 3.98 percent, and the proportion of the preference feed B is 96.02 percent.
Example 2
The livestock manure and the waste straw are used as raw materials for fermentation, and the raw materials are divided into two parts in the fermentation process, wherein one part is added with partial bagasse in the fermentation process, and the other part is added with partial edible fungi in the fermentation process, so that feed A containing bagasse and feed B containing edible fungi are obtained.
Taking an 80cm organic glass tube with the diameter of 1.5cm, marking two dividing lines at symmetrical positions of the inner wall of the organic glass tube along the direction of the organic glass tube, clearly painting the two dividing lines of the inner wall of the organic glass tube by using bright pigments, taking an organic glass plate, cutting an organic glass partition plate which is 85cm long and has the same width as the inner diameter of the organic glass tube, polishing and repairing the edge of the organic glass partition plate, inserting the organic glass partition plate into the organic glass tube along the two dividing lines in the organic glass tube, dividing the organic glass tube into two equally-divided semi-cylindrical spaces, plugging one end of the organic glass tube by gauze, respectively filling different feeds A and B into two parts formed in the organic glass tube, respectively extruding the filled feeds with slight force, after the completion of filling 58cm of feeds, drawing out the partition plate, and vertically placing the organic glass tube for later use, obtaining a complete feeding tube;
selecting 1/2 healthy earthworms with the diameter of the organic glass tube at the position of an annulus, putting the earthworms into a feeding tube filled with two different feeds, plugging the tube head of the feeding tube by a plastic plug with a pinhole-sized vent hole, enabling the earthworms to continuously move to one side of a gauze plug along the feeding tube and take the earthworms, taking the earthworms out when the earthworms take the earthworms and move to the other side of the tube along the feeding tube, putting the feeding tube into an oven, and baking for 60min at the temperature of 53 ℃ to enable the temperature in the feeding tube to be uniform;
heating 1000ml of water to 60 ℃, adding 20g of agar into the water, stirring the mixture to uniformly mix the agar and the water to obtain agar liquid, plugging one end of a feeding pipe, erecting the feeding pipe, pouring the agar liquid into the feeding pipe from the other end of the feeding pipe, filling holes drilled in the feeding pipe by earthworms from top to bottom with the agar liquid, filling gaps between two different feeds and gaps between the two different feeds and the inner wall of an organic glass pipe, standing, naturally cooling or air cooling to room temperature, completing the fixation of the earthworm feeding channel shape after the agar liquid is completely solidified, slowly pushing the fixed part in the feeding pipe out of the feeding pipe, cutting the pipe along the feeding pipe by using a craft knife every 2cm to obtain 29 samples, recording whether the feeding preference of the earthworms tested by the samples is feed A or feed B, and calculating the relative feeding preference of the earthworms according to the feeding preference, selecting a plurality of different earthworms to repeat the operation.
Wherein the proportion of the preference feed A is 43.85%, and the proportion of the preference feed B is 56.15%.
Example 3
The raw materials of the livestock manure and the waste straw are used for fermentation, and the raw materials are divided into two parts in the fermentation process, wherein one part is added with part of edible fungi in the fermentation process, so that the feed A without the edible fungi and the feed B with the edible fungi are obtained.
Taking an 80cm organic glass tube with the diameter of 1.5cm, marking two dividing lines at symmetrical positions of the inner wall of the organic glass tube along the direction of the organic glass tube, clearly painting the two dividing lines of the inner wall of the organic glass tube by using bright pigments, taking an organic glass plate, cutting an organic glass partition plate which is 85cm long and has the same width as the inner diameter of the organic glass tube, polishing and repairing the edge of the organic glass partition plate, inserting the organic glass partition plate into the organic glass tube along the two dividing lines in the organic glass tube, dividing the organic glass tube into two equally-divided semi-cylindrical spaces, plugging one end of the organic glass tube by gauze, respectively filling different feeds A and B into two parts formed in the organic glass tube, respectively extruding the filled feeds with slight force, after filling 62cm of feeds, drawing out the partition plate, and vertically placing the organic glass tube for later use, obtaining a complete feeding tube;
selecting 1/2 healthy earthworms with the diameter of the organic glass tube at the position of an annulus, putting the earthworms into a feeding tube filled with two different feeds, plugging the tube head of the feeding tube by a plastic plug with a pinhole-sized vent hole, enabling the earthworms to continuously move to one side of a gauze plug along the feeding tube and take the earthworms, taking the earthworms out when the earthworms take the earthworms and move to the other side of the tube along the feeding tube, putting the feeding tube into an oven, and baking for 60min at the temperature of 57 ℃ to enable the temperature in the feeding tube to be uniform;
heating 1000ml of water to 60 ℃, adding 20g of agar into the water, stirring the mixture to uniformly mix the agar and the agar to obtain an agar liquid, plugging one end of a feeding pipe, erecting the feeding pipe, pouring the agar liquid into the feeding pipe from the other end of the feeding pipe, filling holes drilled in the feeding pipe by earthworms from top to bottom with the agar liquid, filling gaps between two different feeds and gaps between the two different feeds and the inner wall of an organic glass pipe, standing, naturally cooling or air cooling to room temperature, completing the fixation of the earthworm feeding channel shape after the agar liquid is completely solidified, slowly pushing the fixed part in the feeding pipe out of the feeding pipe, cutting the pipe along the feeding pipe by using a utility knife every 2cm to obtain samples at 31 positions, recording whether the feeding preference of the earthworms tested by the samples at multiple positions is feed A or feed B, and calculating the relative feeding preference of the earthworms according to the feeding percentage, selecting a plurality of different earthworms to repeat the operation.
Wherein the proportion of the preference feed A is 2.78%, and the proportion of the preference feed B is 97.22%.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (8)
1. A method for testing eating preference of earthworms is characterized by comprising the following steps:
(1) manufacturing a feeding tube: taking an 80cm organic glass tube, dividing the space in the organic glass tube into two equal parts by using a partition plate, and plugging one end of the organic glass tube by using gauze;
(2) filling feed: filling different feeds A and B into two parts formed in an organic glass tube respectively, slightly and forcibly extruding the filled feeds respectively, after filling, drawing out the partition plate, and vertically placing the organic glass tube for later use to obtain a complete feeding tube;
(3) testing the feeding preference of earthworms: selecting a healthy earthworm, putting the earthworm into a feeding pipe filled with two different feeds, and plugging the pipe head of the feeding pipe by a plastic plug with a pinhole-sized vent hole, so that the earthworm continuously moves to one side of a gauze plug along the feeding pipe and takes the earthworm;
(4) baking treatment: when the earthworms take food along the feeding pipe and move to the other side of the pipe, the earthworms are taken out, and the feeding pipe is placed into an oven for baking treatment, so that the temperature in the feeding pipe is uniform;
(5) preparing an agar solution: heating 1000ml of water to 60 ℃, adding 20g of agar into the water, and stirring the agar and the agar to uniformly mix the agar and the agar to obtain agar liquid;
(6) fixing a food taking channel: plugging one end of a feeding pipe, erecting the feeding pipe, pouring the agar liquid into the feeding pipe from the other end of the feeding pipe, filling holes drilled in the feeding pipe by earthworms from top to bottom with the agar liquid, filling gaps between two different feeds and gaps between the two different feeds and the inner wall of an organic glass pipe, standing, cooling to room temperature, and finishing the fixation of the shape of an earthworm feeding channel after the agar liquid is completely solidified;
(7) cutting a sample: slowly pushing the fixed part in the feeding tube out of the feeding tube, and cutting off the part along the tube opening of the feeding tube by using an art designer knife when pushing out the part to obtain a plurality of samples;
(8) quantitative recording of feeding preferences: the eating preference of the earthworms tested by a plurality of samples is recorded as feed A or feed B, and the relative eating preference of the earthworms is calculated according to percentage.
2. The method for testing the feeding preference of the earthworms according to claim 1, wherein the step (1) of dividing the space inside the organic glass tube into two equal parts by using the partition plate comprises the following steps:
a. marking two dividing lines along the direction of the organic glass tube at symmetrical positions of the inner wall of the organic glass tube;
b. two parting lines on the inner wall of the organic glass tube are clearly coated by bright pigments;
c. taking an organic glass plate, and cutting out an organic glass partition plate which is 85cm long and has the same width as the inner diameter of the organic glass pipe;
d. the edge of the organic glass partition plate is polished and repaired, and the organic glass partition plate is inserted into the organic glass tube along two partition lines in the organic glass tube, so that the organic glass tube is divided into two equally-divided semi-cylindrical spaces.
3. The method for testing the feeding preference of the earthworms according to claim 1, wherein the diameter of the plexiglass tube in the step (1) is 1cm to 2 cm.
4. The method for testing feeding preference of earthworms according to claim 1, wherein the depth of the feed filling in the step (2) is 60cm ± 2 cm.
5. The method of claim 1, wherein the annulus of the earthworms selected in step (3) has a diameter of 1/2 mm the inner diameter of the plexiglas tube.
6. The method for testing the feeding preference of the earthworms according to claim 1, wherein the temperature of the roasting treatment in the step (4) is 55 ℃ ± 2 ℃, and the time of the roasting treatment is 60 min.
7. The method for testing the feeding preference of the earthworms according to claim 1, wherein the cooling to the room temperature in the step (6) is natural cooling or wind cooling.
8. The method for testing feeding preference of earthworms according to claim 1, wherein the sample cut in the step (7) is 2cm thick.
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| CN110646568A (en) * | 2019-10-12 | 2020-01-03 | 中国农业科学院麻类研究所 | Feed palatability screening method based on animal group behaviors |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102007877A (en) * | 2010-09-19 | 2011-04-13 | 中国科学院海洋研究所 | Method for measuring ingestion preference degrees of sea cucumbers |
| CN205176014U (en) * | 2015-08-27 | 2016-04-20 | 福建农林大学 | Test device is avoided to multi -chambered type earthworm |
| CN106857409A (en) * | 2017-01-17 | 2017-06-20 | 张家港市永联菜篮农业专业合作社 | The lasting diffusion propagating system of Encarsia formosa |
| CN108850664A (en) * | 2018-05-08 | 2018-11-23 | 赵善廷 | A kind of preparation method using raw earthworm processing feed |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20170036674A (en) * | 2017-03-27 | 2017-04-03 | 임운영 | Large scale apparatus for growing soil life |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102007877A (en) * | 2010-09-19 | 2011-04-13 | 中国科学院海洋研究所 | Method for measuring ingestion preference degrees of sea cucumbers |
| CN205176014U (en) * | 2015-08-27 | 2016-04-20 | 福建农林大学 | Test device is avoided to multi -chambered type earthworm |
| CN106857409A (en) * | 2017-01-17 | 2017-06-20 | 张家港市永联菜篮农业专业合作社 | The lasting diffusion propagating system of Encarsia formosa |
| CN108850664A (en) * | 2018-05-08 | 2018-11-23 | 赵善廷 | A kind of preparation method using raw earthworm processing feed |
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