CN110038149A - A kind of inactivation of biology laboratory waste cell and Cytology Lab improve sterilization method - Google Patents
A kind of inactivation of biology laboratory waste cell and Cytology Lab improve sterilization method Download PDFInfo
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- CN110038149A CN110038149A CN201910322052.3A CN201910322052A CN110038149A CN 110038149 A CN110038149 A CN 110038149A CN 201910322052 A CN201910322052 A CN 201910322052A CN 110038149 A CN110038149 A CN 110038149A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L11/00—Methods specially adapted for refuse
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultra-violet radiation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
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Abstract
The present invention is that a kind of biology laboratory waste cell inactivates and Cytology Lab improves sterilization method.A kind of biology laboratory waste cell ablation method, comprising the following steps: after addition disinfectant 1 is inactivated into waste cell, then discharged；Wherein, the disinfectant 1 is 84 disinfection liquors.A kind of Cytology Lab improvement sterilization method is also disclosed in the present invention.A kind of biology laboratory waste cell inactivation of the present invention and Cytology Lab improve sterilization method; it has determined and had killed the optimal concentration value of cell using 84 disinfectants within 1 minute short time; in conjunction with existing 75% alcohol and ultraviolet sterilization; it can be preferably applied for Cytology Lab disinfection; the especially processing of the disinfection of super-clean bench and discarded living cells; the pollution between biological sample is avoided, environment is protected.
Description
Technical field
Present invention relates particularly to a kind of inactivations of biology laboratory waste cell and Cytology Lab to improve sterilization method.
Background technique
In biology laboratory, cell culture is common method, and largely discarded living cells (its is often generated in experimentation
In include viral transfected cells cell, cancer cell), if entering sewer without inactivation treatment direct emission, be easy to cause ring
Border pollution, and the health of surrounding population is caused potentially to threaten.In addition, if waste cell is easy to appear cell without inactivation
Pollution between Bioexperiment.And the country has no commercially available preparation and related experiment and method for living cells inactivation at present
Report.
The disinfection of Cell Lab at present still uses the methods of 75% alcohol, bromogeramine, formaldehyde fumigation, especially
The sterilization method that the disinfection of super-clean bench uses is mostly 75% alcohol sprinkling+ultraviolet light irradiation 30min, but due to certain thin
The vitality of born of the same parents is stronger, finds that this routine disinfection can not kill completely it with many years of experience in this laboratory, easily causes thin
Cross contamination between born of the same parents' Bioexperiment, or even cause the variation of cell and influence experimental result, it is therefore necessary to existing experiment
The sterilization method of room is improved.
Accordingly, the present invention proposes a kind of ablation method of novel, cheap effective biology laboratory waste cell, and
The pollution of biology laboratory, environment-friendly high-efficiency can be preferably reduced in conjunction with existing Cell Lab sterilization method.
Summary of the invention
The purpose of the present invention is to provide a kind of inactivations of biology laboratory waste cell and Cytology Lab to improve disinfection side
Method, this method match a kind of common disinfectant (84) according to a certain percentage, sterilizes for Cytology Lab, is handled with this
For the waste cell in laboratory to the disinfection applied to Cytology Lab, the pollution reduced between cell experiment is especially intercellular
Cross contamination.
To achieve the goals above, used technical solution are as follows:
A kind of biology laboratory waste cell ablation method, comprising the following steps:
After into waste cell, addition disinfectant 1 is inactivated, then discharged；
Wherein, the disinfectant 1 is 84 disinfection liquors.
Further, during the initial sterilization, the volume of 84 thimerosal stostes and water in the disinfectant 1
Than for 1:499.
Further, during the initial sterilization, the disinfecting time of the disinfectant 1 is 1-5min.
Further, during the initial sterilization, disinfecting time 1min.
A kind of Cytology Lab improves sterilization method, method the following steps are included:
It is carried out disinfection using disinfectant 1 to Cell Lab, wherein the disinfectant 1 is 84 disinfection liquors.
Further, in the disinfecting process, 84 thimerosal stostes and the volume ratio of water are in the disinfectant 1
1:499。
Further, in the disinfecting process, the disinfecting time of the disinfectant 1 is 1-5min.
Further, in the disinfecting process, disinfecting time 1min.
Further, when carrying out disinfection to the super-clean bench in the Cell Lab, further includes: using disinfectant 2 with
Ultraviolet light irradiation carries out disinfection to super-clean bench；The disinfectant 2 is 75% alcohol.
Further, the time of ultraviolet irradiation is 30min.
Compared with prior art, the invention has the beneficial effects that:
1, what this tested selection is clear cell carcinoma of kidney cell, esophageal cancer cell, synovial sarcoma, the ovum of vitality tanacity
Nest cancer, human rhabdomyosarcoma cells, 84 disinfectant stock solutions and water are 1:499 (concentration 100mg/L) with these cells of comparison
Killing effect is very good, can preferably be used for the inactivation of waste cell during experimental implementation, and then be used for laboratory cell
Between sterilization method improvement.
2,84 disinfectants are sold and cheaply extensively in the market, therefore the method for this test is cheap, has higher popularization to answer
With value.
3, this method can combine sterilization method (75% alcohol sprinkling+purple that current most domestic laboratory uses
Outer light irradiation 30min), the Disinfection Effect in laboratory more preferably, greatly reduces the cross contamination between cell experiment after joint, improves
Conventional efficient saves experimental material, reduces environmental pollution.
A kind of biology laboratory waste cell inactivation of the present invention and Cytology Lab improve sterilization method, it is determined that
Common 84 disinfectant kills the optimal concentration value of cell within 1 minute short time, to be applied to the inactivation of waste cell and thin
Cell disinfection, the mainly disinfection of super-clean bench and cell incubator.
Super-clean bench sequence of sterilization are as follows: (1) stoste and water be 84 disinfectants of 1:499 (concentration 100mg/L) wipe,
Clear water wipes thimerosal after 1min, the sprinkling wiping of (2) 75% alcohol, (3) ultraviolet lamp disinfection 30min.
Cell incubator sequence of sterilization are as follows: (1) stoste and water are that 84 disinfectants of 1:499 (concentration 100mg/L) are wiped
It wipes, clear water wipes thimerosal after 1min, the sprinkling wiping of (2) 75% alcohol.
Detailed description of the invention
When Fig. 1 is reaction 1 minute, inhibition of the disinfectant 1 of various concentration to clear cell carcinoma of kidney cell strain (786-0)
Rate；In figure, ordinate is inhibiting rate (%), and abscissa is the volume ratio of 84 stostes and water；
When Fig. 2 is reaction 5 minutes, inhibition of the disinfectant 1 of various concentration to clear cell carcinoma of kidney cell strain (786-0)
Rate；In figure, ordinate is inhibiting rate (%), and abscissa is the volume ratio of 84 stostes and water；
When Fig. 3 is reaction 1 minute, the disinfectant 1 of 0mg/L, 20mg/L, 40mg/L are to clear cell carcinoma of kidney cell strain
The influence of cellular morphology variation before and after (786-6)；In figure, above 3 width be reaction before cellular morphology figure, below 3 width be reaction
Cellular morphology figure afterwards；The figure of 1-3 column successively uses the volume ratio of 84 stostes and water disappearing for 0,1:2499,1:1249
Toxic agent 1；When Fig. 4 is reaction 1 minute, the disinfectant 1 of 60mg/L, 80mg/L, 100mg/L are to clear cell carcinoma of kidney cell strain
The influence of cellular morphology variation before and after (786-6)；In figure, above 3 width be reaction before cellular morphology figure, below 3 width be anti-
Cellular morphology figure after answering；The figure of 1-3 column successively uses the volume ratio of 84 stostes and water for 1:832,1:624,1:499
Disinfectant 1；
When Fig. 5 is reaction 5 minutes, the disinfectant 1 of 0mg/L, 20mg/L, 40mg/L are to clear cell carcinoma of kidney cell strain
The influence of cellular morphology variation before and after (786-6)；In figure, above 3 width be reaction before cellular morphology figure, below 3 width be reaction
Cellular morphology figure afterwards；The figure of 1-3 column successively uses the volume ratio of 84 stostes and water disappearing for 0,1:2499,1:1249
Toxic agent 1；
When Fig. 6 is reaction 5 minutes, the disinfectant 1 of 60mg/L, 80mg/L, 100mg/L are to clear cell carcinoma of kidney cell strain
The influence of cellular morphology variation before and after (786-6)；In figure, above 3 width be reaction before cellular morphology figure, below 3 width be reaction
Cellular morphology figure afterwards；The figure of 1-3 column successively uses the volume ratio of 84 stostes and water for 1:832,1:624,1:499
Disinfectant 1；
Fig. 7 is inhibiting rate of the disinfectant 1 to clear cell carcinoma of kidney cell strain (786-0) of various concentration；In figure, indulges and sit
It is designated as inhibiting rate (%), abscissa is the volume ratio of 84 stostes and water；
When Fig. 8 is reaction 1 minute, inhibition of the disinfectant 1 of various concentration to clear cell carcinoma of kidney cell strain (ACHN)
Rate；In figure, ordinate is inhibiting rate (%), and abscissa is the volume ratio of 84 stostes and water；
When Fig. 9 is reaction 1 minute, the inhibiting rate of the disinfectant 1 of various concentration to esophageal cancer cell strain (109)；In figure,
Ordinate is inhibiting rate (%), and abscissa is the volume ratio of 84 stostes and water；
When Figure 10 is reaction 1 minute, the inhibiting rate of the disinfectant 1 of various concentration to esophageal cancer cell strain (9706)；Figure
In, ordinate is inhibiting rate (%), and abscissa is the volume ratio of 84 stostes and water；
When Figure 11 is reaction 1 minute, the inhibiting rate of the disinfectant 1 of various concentration to synovial sarcoma cells strain (SW982)；
In figure, ordinate is inhibiting rate (%), and abscissa is the volume ratio of 84 stostes and water；
When Figure 12 is reaction 1 minute, the inhibiting rate of the disinfectant 1 of various concentration to Ovarian Cancer Cells (A2980)；Figure
In, ordinate is inhibiting rate (%), and abscissa is the volume ratio of 84 stostes and water；
When Figure 13 is reaction 1 minute, the inhibiting rate of the disinfectant 1 of various concentration to rhabdomyosarcoma (RD)；In figure, indulge
Coordinate is inhibiting rate (%), and abscissa is the volume ratio of 84 stostes and water.
Specific embodiment
In order to which the present invention is further explained, a kind of inactivation of biology laboratory waste cell and Cytology Lab improve disinfection side
Method reaches expected goal of the invention, in conjunction with the preferred embodiment, discarded to a kind of biology laboratory proposed according to the present invention
Cell inactivation and Cytology Lab improve sterilization method, and specific embodiment, structure, feature and its effect are described in detail such as
Afterwards.In the following description, what different " embodiment " or " embodiment " referred to is not necessarily the same embodiment.In addition, one or more
Special characteristic, structure or feature in a embodiment can be combined by any suitable form.
Elaborate a kind of inactivation of biology laboratory waste cell of the present invention and Cytology Lab improvement sterilization method it
Before, it is necessary to the raw material etc. referred in the present invention is described further, to reach better effect.
84 thimerosals are one kind with sodium hypochlorite chlorine-containing disinfectant as main component, are mainly used for various body surfaces
With the disinfection of environment etc..84 thimerosals can kill Escherichia coli, be suitable for family, hotel, hospital, restaurant and other public fields
Surface disinfection.It can be penetrated into the activity of bacterium internal sabotage bacterial metabolism enzyme by cell wall and cause it dead
It dies, has very strong killing effect to bacterial spore, first, hepatitis B, AIDS virus, poliovirus.It has reported
84 thimerosals are crossed to be respectively that 2.5mg/L and 1mg/L can respectively can be to pair when 1 minute and 5 minutes containing effective chlorine in road
Hemolytic vibrios has killing effect, and it can kill tuberculosis point for 30 minutes in 84 thimerosal of low concentration (200-400mg/L)
Branch bacillus.
It is useless to a kind of biology laboratory of the present invention below in conjunction with specific embodiment after having understood above-mentioned raw materials etc.
It abandons cell inactivation and Cytology Lab improvement sterilization method is further described in detail:
One embodiment
Embodiment 1.
A kind of processing method of waste cell the following steps are included:
After into waste cell, addition disinfectant 1 (84 disinfection liquor) is inactivated, then discharged.
Preferably, during the initial sterilization, the volume ratio of 84 thimerosal stostes and water in the disinfectant 1
For 1:499, i.e. mass concentration is 100mg/L, disinfecting time 1-5min.When the mass concentration of disinfectant 1 is 100mg/L,
Only need 1-5min that can eliminate most cells.
It is further preferred that the disinfecting time of the disinfectant 1 is 1min during the initial sterilization.
100mg/L is the optimal concentration for killing cell in (1 minute) in the shortest time of disinfectant (84).
A kind of processing method of waste cell of the present invention, it is determined that common 84 disinfectant within 1 minute short time
The optimal concentration value of cell is killed, to kill the cell that Cytology Lab is retained in iuntercellular especially super-clean bench in operation,
To reduce intercellular pollution.
Embodiment 2.
A kind of Cell Lab sterilization method, comprising the following steps:
Initial sterilization is carried out to Cell Lab using disinfectant 1 (84 disinfection liquor), then uses disinfectant 2 (75%
Alcohol) carry out secondary sterilization.Disinfection emphasis is super-clean bench, and super-clean bench using disinfectant 1 after being carried out disinfection, then uses disinfection
Agent 2 (75% alcohol) carries out secondary sterilization, finally ultraviolet lamp disinfection 30min again.
Preferably, during initial sterilization, 84 thimerosal stostes and the volume ratio of water are 1 in the disinfectant 1:
499, i.e. mass concentration is 100mg/L, disinfecting time 1-5min.When the mass concentration of disinfectant 1 is 100mg/L, it is only necessary to
1-5min can eliminate most cells.
It is further preferred that the disinfecting time of the disinfectant 1 is 1min during the initial sterilization.
100mg/L is the optimal concentration for killing cell in (1 minute) in the shortest time of disinfectant (84).
A kind of Cell Lab sterilization method of the present invention, it is determined that common 84 disinfectant within 1 minute short time
The optimal concentration value of cell is killed, is retained in the thin of iuntercellular, especially super-clean bench in operation to kill Cytology Lab
Born of the same parents, to reduce intercellular pollution.
Two experiments
1, the optimal concentration value within common disinfectants (84) short time (1) purpose: has been determined in polyclonal cellular
It is most strong to the killing effect of various kinds of cell, to be applied to the processing and Cytology Lab disinfection of cell.
(2) preparation of disinfectant 1
Disinfectant 1 with method it is as shown in table 1.
84 disinfectants of 1 various concentration of table
2, clear cell carcinoma of kidney cell strain
(1) material: using cell culture technology, carries out cell proliferation experiment (CCK8) to renal carcinoma cell line.
(2) inhibiting rate of the disinfectant 1 of various concentration to clear cell carcinoma of kidney cell strain (786-0)
1. method: according to the method for table 1, preparing 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/
L, the disinfectant 1 of 140mg/L is added separately to clear cell carcinoma of kidney cell strain (786-0), carries out cell proliferation experiment
(CCK8)；And blank pair is set.Reaction time is 1 minute, detects and observes the influence to the proliferative conditions of cell.
As a result: the result of the inhibiting rate of different 1 concentration versus cells of disinfectant is as shown in Figure 1.As shown in Fig. 1,1 point is reacted
Zhong Shi, cell inhibitory rate rises with the rising of the concentration of disinfectant 1, when reaching 60mg/L, cell inhibitory rate
It improves and starts to become slow；Until cell inhibitory rate improves few when 100mg/L, and 1 concentration of disinfectant is 60-
When 100mg/L, cell inhibitory rate is preferable.
2. method: according to the method for table 1, prepare the disinfectant 1 of 15mg/L, 20mg/L, 40mg/L, 60mg/L, respectively plus
Enter to clear cell carcinoma of kidney cell strain (786-0), carries out cell proliferation experiment (CCK8)；And blank pair is set.Reaction time is
It 5 minutes, detects and observes the influence to the proliferative conditions of cell.
As a result: as shown in Fig. 2, cell inhibitory rate rises with the rising of the concentration of disinfectant 1 when reacting 5 minutes,
When reaching 20-40mg/L, cell inhibitory rate is hardly improved；When reaching 40-60mg/L, cell inhibitory rate
Raising become slowly, and when reaching 60mg/L, cell inhibitory rate highest.
It is tested and is found by CCK8, the increase with the concentration of disinfectant 1 gradually increases and decreases until flat the inhibiting rate of cell
The platform phase, and reaction 5 minutes can be seen that by suppression curve, cell is in plateau when 60mg/L；Reaction 1 minute,
When at 100mg/L be in plateau, and 1 concentration of disinfectant be 60-100mg/L when, cell inhibitory rate highest, institute as shown in Figure 1, Figure 2
Show.
(3) influence of 1 concentration of disinfectant to clear cell carcinoma of kidney cell (786-6) form
1. method: according to the method for table 1, preparing the disinfectant of 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L
1, it is added separately to clear cell carcinoma of kidney cell (786-6), carries out cell proliferation experiment (CCK8)；And blank pair is set.Reaction
Time is 1 minute, detects and observes the influence to cellular morphology.
As a result: after reaction 1 minute, clear cell carcinoma of kidney cell (786-6) uses cell shape before and after various concentration disinfectant
State variation is as shown in Figure 3 and Figure 4.
As can be seen from figs. 3 and 4 disinfectant 1 has a deactivation to clear cell carcinoma of kidney cell, and cell quantity is with disappearing
The rising of the concentration of toxic agent 1 and reduce, closeness reduce.
2. method: according to the method for table 1, preparing the disinfectant of 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L
1, it is added separately to clear cell carcinoma of kidney cell (786-6), carries out cell proliferation experiment (CCK8)；And blank pair is set.Reaction
Time is 5 minutes, detects and observes the influence to cellular morphology.
As a result: after five minutes, clear cell carcinoma of kidney cell (786-6) uses cell shape before and after various concentration disinfectant for reaction
State variation is as shown in Figure 5 and Figure 6.
By Fig. 3 and Fig. 6 it is found that disinfectant 1 has deactivation to clear cell carcinoma of kidney cell, cell becomes smaller, and cell number
Amount reduced with the rising of the concentration of disinfectant 1, closeness reduce, when the concentration of disinfectant 1 be 60mg/L or more when
It waits, it is best to the deactivation of cell.
It is compared by the variation to cellular morphology before dosing, discovery is sent out with the form of the increase cell of decontaminant concentration
Changing cell when to optium concentration cracks completely, and the concentration that the cell of reaction 1 minute cracks completely is in 60-100mg/L
Between, 5 minutes are reacted in 60mg/L, such as Fig. 3-6.
(4) inhibiting rate of 1 pair of disinfectant different clear cell carcinoma of kidney cell strains
1. method: according to the method for table 1, preparing 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/
L, the disinfectant 1 of 140mg/L is added separately to clear cell carcinoma of kidney cell strain (786-0), carries out cell proliferation experiment
(CCK8)；And blank pair is set, detect and observes the influence to the proliferative conditions of cell.
As a result: the result of the inhibiting rate of different 1 concentration versus cells of disinfectant is as shown in Figure 7.As shown in Fig. 7, cell inhibits
Rate rises with the rising of the concentration of disinfectant 1, and when reaching 60mg/L, the raising of cell inhibitory rate becomes very
Slowly；There are also the concentration of disinfectant 1 be 60mg/L or more when, cell inhibitory rate is preferable, and approaches.
2. method: preparing the disinfection of 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L, 140mg/L
Agent 1 is added separately to clear cell carcinoma of kidney cell strain (ACHN), is carried out cell proliferation experiment (CCK8)；And blank pair is set,
It detects and observes the influence to the proliferative conditions of cell.
As a result: the result of the inhibiting rate of different 1 concentration versus cells of disinfectant is as shown in Figure 8.As shown in Fig. 8, cell inhibits
Rate rises with the rising of the concentration of disinfectant 1, and when reaching 60mg/L, the raising of cell inhibitory rate becomes very
Slowly；There are also the concentration of disinfectant 1 be 60mg/L or more when, cell inhibitory rate is preferable, and very close.
3, esophageal cancer cell
(1) material: using cell culture technology, carries out cell proliferation experiment (CCK8) to esophageal cancer cell.
(2) inhibiting rate of 1 concentration of disinfectant to esophageal cancer cell strain (109)
Method: according to the method for table 1, prepare 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L,
The disinfectant 1 of 140mg/L is added separately to esophageal cancer cell strain (109), it carries out cell proliferation experiment (CCK8)；And it is arranged
Blank pair detects and observes the influence to the proliferative conditions of cell.
As a result: the result of the inhibiting rate of different 1 concentration versus cells of disinfectant is as shown in Figure 9.As shown in Fig. 9, cell inhibits
Rate rises with the rising of the concentration of disinfectant 1, and when reaching 60mg/L, the raising of cell inhibitory rate becomes very
Slowly；There are also the concentration of disinfectant 1 be 60mg/L or more when, cell inhibitory rate is preferable, and very close；Cellular morphology
It is become round by intensive shuttle shape, short shuttle shape to clasmatosis and is dissolved, living cells disappears.
(3) inhibiting rate of 1 concentration of disinfectant to esophageal cancer cell strain (9706)
Method: according to the method for table 1, prepare 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L,
The disinfectant 1 of 140mg/L is added separately to esophageal cancer cell strain (9706), is carried out cell proliferation experiment (CCK8)；And it is arranged
Blank pair detects and observes the influence to the proliferative conditions of cell.
As a result: the results are shown in Figure 10 for the inhibiting rate of different 1 concentration versus cells of disinfectant.As shown in Figure 10, cell suppression
Rate processed rises with the rising of the concentration of disinfectant 1, and when reaching 60mg/L, the raising of cell inhibitory rate becomes ten
Divide slow；There are also the concentration of disinfectant 1 be 60mg/L or more when, cell inhibitory rate is preferable, and approaches；Cellular morphology by
Intensive shuttle shape, short shuttle shape, which become round to clasmatosis, to be dissolved, and living cells disappears.
4, synovial sarcoma cells
(1) material: using cell culture technology, carries out cell proliferation experiment (CCK8) to synovial sarcoma cells.
(2) inhibiting rate of 1 concentration of disinfectant to synovial sarcoma cells strain (SW982)
Method: according to the method for table 1, prepare 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L,
The disinfectant 1 of 140mg/L is added separately to synovial sarcoma cells strain (SW982), is carried out cell proliferation experiment (CCK8)；And it sets
It is white right to empty, and detects and observes the influence to the proliferative conditions of cell.
As a result: the result of the inhibiting rate of different 1 concentration versus cells of disinfectant is as shown in figure 11.As shown in Figure 11, cell suppression
Rate processed rises with the rising of the concentration of disinfectant 1, and when reaching 20mg/L, the raising of cell inhibitory rate becomes ten
Divide slow；And the concentration of disinfectant 1, when being gradually increased, cell inhibitory rate is preferable, and approaches；Cellular morphology is by intensive shuttle
Shape, short shuttle shape, which become round to clasmatosis, to be dissolved, and living cells disappears.
5, ovarian cancer cell
(1) material: using cell culture technology, carries out cell proliferation experiment (CCK8) to ovarian cancer cell.
(2) inhibiting rate of 1 concentration of disinfectant to Ovarian Cancer Cells (A2980)
Method: according to the method for table 1, prepare 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L,
The disinfectant 1 of 140mg/L is added separately to Ovarian Cancer Cells (A2980), is carried out cell proliferation experiment (CCK8)；And it is arranged
Blank pair detects and observes the influence to the proliferative conditions of cell.
As a result: the result of the inhibiting rate of different 1 concentration versus cells of disinfectant is as shown in figure 12.As shown in Figure 12, cell suppression
Rate processed rises with the rising of the concentration of disinfectant 1, and when reaching 60mg/L, the raising of cell inhibitory rate becomes ten
Divide slow；There are also the concentration of disinfectant 1 be 60mg/L or more when, cell inhibitory rate is preferable, and very close；Cell shape
State, which is become round by intensive shuttle shape, short shuttle shape to clasmatosis, to be dissolved, and living cells disappears.
6, rhabdomyosarcoma
(1) material: using cell culture technology, carries out cell proliferation experiment (CCK8) to rhabdomyosarcoma.
(2) inhibiting rate of 1 concentration of disinfectant to rhabdomyosarcoma (RD)
Method: according to the method for table 1, prepare 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L,
The disinfectant 1 of 140mg/L is added separately to rhabdomyosarcoma (RD), is carried out cell proliferation experiment (CCK8)；And blank is set
It is right, it detects and observes the influence to the proliferative conditions of cell.
As a result: the result of the inhibiting rate of different 1 concentration versus cells of disinfectant is as shown in figure 13.As shown in Figure 13, cell suppression
Rate processed rises with the rising of the concentration of disinfectant 1, and when reaching 80mg/L, the raising of cell inhibitory rate becomes ten
Divide slow；There are also the concentration of disinfectant 1 be 60mg/L or more when, cell inhibitory rate is preferable, and approaches；Cellular morphology by
Intensive shuttle shape, short shuttle shape, which become round to clasmatosis, to be dissolved, and living cells disappears.
By testing 3-6, killed when 1 minute concentration is in 60-100mg/L carefully we prefer that having gone out the disinfectant (84)
The effect of born of the same parents is preferable, best in the effect that 100mg/L or so kills cell, such as Fig. 9-13.
By in various kinds of cell utilize proliferation experiment confirmatory experiment as a result, discovery clear cell carcinoma of kidney, the cancer of the esophagus,
Synovial sarcoma, Ovarian Cancer Cells, 84 disinfectants all exists after a variety of cancer cell inner reactions 1 minute of rhabdomyosarcoma cancer
It is best that cytosis is killed at 100mg/L, i.e., in 84 original solutions: total solution volume is 1:500.
7, it summarizes
Using renal carcinoma cell line (786-0, ACHN), esophageal cancer cell (109,9706), synovial sarcoma cells (SW982),
Ovarian Cancer Cells (A2980), rhabdomyosarcoma cancer cell line (RD) are thin required for cultivating by cell culture technology
Born of the same parents.
It is tested by CCK8,1. detection reacts 1 minute after dosing, the proliferative conditions of cell after five minutes are so that it is determined that more
Add the concentration of accurate disinfectant；2. by reacting 1 minute, 5 minutes cell shapes before and after comparison addition various concentration disinfectant 1
The change of state；3. verifying in different cell strains 84 effect by proliferation experiment.It has determined within 1 minute short time often
See that 84 disinfectants kill the optimal concentration value of cell, is sterilized to be applied to Cytology Lab, especially super-clean bench and cell incubator
Disinfection (disinfection super-clean bench sequence can be 84 disinfectants of 100mg/L, 75% alcohol and ultraviolet) and waste cell processing.
The above is only the preferred embodiment of the embodiment of the present invention, not makees any shape to the embodiment of the present invention
Limitation in formula, any simple modification to the above embodiments of technical spirit according to an embodiment of the present invention, equivalent variations
With modification, in the range of still falling within technical solution of the embodiment of the present invention.
Claims (10)
1. a kind of biology laboratory waste cell ablation method, which is characterized in that the described method comprises the following steps:
After into waste cell, addition disinfectant 1 is inactivated, then discharged；
Wherein, the disinfectant 1 is 84 disinfection liquors.
2. biology laboratory waste cell ablation method according to claim 1, which is characterized in that
During the initial sterilization, 84 thimerosal stostes and the volume ratio of water are 1:499 in the disinfectant 1.
3. biology laboratory waste cell ablation method according to claim 2, which is characterized in that
During the initial sterilization, the disinfecting time of the disinfectant 1 is 1-5min.
4. biology laboratory waste cell ablation method according to claim 3, which is characterized in that
During the initial sterilization, disinfecting time 1min.
5. a kind of Cytology Lab improves sterilization method, which is characterized in that the described method comprises the following steps:
It is carried out disinfection using disinfectant 1 to Cell Lab, wherein the disinfectant 1 is 84 disinfection liquors.
6. Cytology Lab according to claim 5 improves sterilization method, which is characterized in that
In the disinfecting process, 84 thimerosal stostes and the volume ratio of water are 1:499 in the disinfectant 1.
7. Cytology Lab according to claim 6 improves sterilization method, which is characterized in that
In the disinfecting process, the disinfecting time of the disinfectant 1 is 1-5min.
8. Cytology Lab according to claim 7 improves sterilization method, which is characterized in that
In the disinfecting process, disinfecting time 1min.
9. Cytology Lab according to claim 5 improves sterilization method, which is characterized in that
When carrying out disinfection to the super-clean bench in the Cell Lab, further includes: irradiated using disinfectant 2 and ultraviolet light to super
Net platform carries out disinfection；The disinfectant 2 is 75% alcohol.
10. Cytology Lab according to claim 9 improves sterilization method, which is characterized in that
The time of ultraviolet irradiation is 30min.
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| CN112587682A (en) * | 2020-11-20 | 2021-04-02 | 福建傲农生物科技集团股份有限公司 | Method for removing aerosol pollution in laboratory |
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