CN110037104A - A kind of wheat stores the preparation method of compound bacteria inhibition tablet - Google Patents
A kind of wheat stores the preparation method of compound bacteria inhibition tablet Download PDFInfo
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- CN110037104A CN110037104A CN201910262420.XA CN201910262420A CN110037104A CN 110037104 A CN110037104 A CN 110037104A CN 201910262420 A CN201910262420 A CN 201910262420A CN 110037104 A CN110037104 A CN 110037104A
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- bacteria inhibition
- wheat
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- 150000001875 compounds Chemical class 0.000 title claims abstract description 53
- 241000894006 Bacteria Species 0.000 title claims abstract description 52
- 230000005764 inhibitory process Effects 0.000 title claims abstract description 51
- 241000209140 Triticum Species 0.000 title claims abstract description 37
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 239000000284 extract Substances 0.000 claims abstract description 33
- 239000000843 powder Substances 0.000 claims abstract description 33
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 30
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 30
- 239000000463 material Substances 0.000 claims abstract description 21
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 13
- 244000269722 Thea sinensis Species 0.000 claims abstract 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 238000012545 processing Methods 0.000 claims description 23
- 239000001522 artemisia absinthium l. herb extract Substances 0.000 claims description 21
- 229940119569 wormwood extract Drugs 0.000 claims description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 14
- 239000000287 crude extract Substances 0.000 claims description 14
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 13
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 13
- 239000012153 distilled water Substances 0.000 claims description 13
- 239000001630 malic acid Substances 0.000 claims description 13
- 235000011090 malic acid Nutrition 0.000 claims description 13
- 238000009835 boiling Methods 0.000 claims description 12
- 235000013305 food Nutrition 0.000 claims description 11
- 241000238631 Hexapoda Species 0.000 claims description 10
- 235000019441 ethanol Nutrition 0.000 claims description 10
- 238000007710 freezing Methods 0.000 claims description 10
- 230000008014 freezing Effects 0.000 claims description 10
- 238000000746 purification Methods 0.000 claims description 10
- 241000607479 Yersinia pestis Species 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 8
- 235000009051 Ambrosia paniculata var. peruviana Nutrition 0.000 claims description 7
- 235000003097 Artemisia absinthium Nutrition 0.000 claims description 7
- 240000001851 Artemisia dracunculus Species 0.000 claims description 7
- 235000017731 Artemisia dracunculus ssp. dracunculus Nutrition 0.000 claims description 7
- 235000003261 Artemisia vulgaris Nutrition 0.000 claims description 7
- 239000001138 artemisia absinthium Substances 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims description 7
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 6
- 238000002845 discoloration Methods 0.000 claims description 6
- 239000004744 fabric Substances 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 239000004615 ingredient Substances 0.000 claims description 6
- GCLGEJMYGQKIIW-UHFFFAOYSA-H sodium hexametaphosphate Chemical compound [Na]OP1(=O)OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])O1 GCLGEJMYGQKIIW-UHFFFAOYSA-H 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 238000000527 sonication Methods 0.000 claims description 6
- 238000002137 ultrasound extraction Methods 0.000 claims description 6
- 235000020097 white wine Nutrition 0.000 claims description 6
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims description 5
- 238000001704 evaporation Methods 0.000 claims description 5
- 230000008020 evaporation Effects 0.000 claims description 5
- JEIPFZHSYJVQDO-UHFFFAOYSA-N ferric oxide Chemical compound O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 4
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 claims description 4
- 229940048086 sodium pyrophosphate Drugs 0.000 claims description 4
- 235000019818 tetrasodium diphosphate Nutrition 0.000 claims description 4
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 claims description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 2
- 239000000654 additive Substances 0.000 claims description 2
- 230000000996 additive effect Effects 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- MOMKYJPSVWEWPM-UHFFFAOYSA-N 4-(chloromethyl)-2-(4-methylphenyl)-1,3-thiazole Chemical compound C1=CC(C)=CC=C1C1=NC(CCl)=CS1 MOMKYJPSVWEWPM-UHFFFAOYSA-N 0.000 claims 1
- 235000019983 sodium metaphosphate Nutrition 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 14
- 238000003860 storage Methods 0.000 abstract description 12
- 235000013339 cereals Nutrition 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 244000052769 pathogen Species 0.000 abstract description 2
- 230000001717 pathogenic effect Effects 0.000 abstract description 2
- 241001122767 Theaceae Species 0.000 description 22
- 239000000243 solution Substances 0.000 description 19
- 241000132177 Aspergillus glaucus Species 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 235000010894 Artemisia argyi Nutrition 0.000 description 4
- 244000030166 artemisia Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000004806 packaging method and process Methods 0.000 description 4
- 231100000481 chemical toxicant Toxicity 0.000 description 3
- 239000000571 coke Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 230000000857 drug effect Effects 0.000 description 3
- 239000001488 sodium phosphate Substances 0.000 description 3
- 229910000162 sodium phosphate Inorganic materials 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000003440 toxic substance Substances 0.000 description 3
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Chemical compound P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000005898 Fenoxycarb Substances 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000748 compression moulding Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- HJUFTIJOISQSKQ-UHFFFAOYSA-N fenoxycarb Chemical compound C1=CC(OCCNC(=O)OCC)=CC=C1OC1=CC=CC=C1 HJUFTIJOISQSKQ-UHFFFAOYSA-N 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000003958 fumigation Methods 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B9/00—Preservation of edible seeds, e.g. cereals
- A23B9/16—Preserving with chemicals
- A23B9/24—Preserving with chemicals in the form of liquids or solids
- A23B9/26—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/18—Extraction of water soluble tea constituents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/20—Agglomerating; Granulating; Tabletting
- A23P10/28—Tabletting; Making food bars by compression of a dry powdered mixture
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses the preparation methods that a kind of wheat stores compound bacteria inhibition tablet, are related to View of Grain Storage Technoiogy field.The present invention includes preparing the process of tea polyphenol extract object powder and compound bacteria inhibition tablet, the present invention mixes tea polyphenol extract object with a variety of natural safe antibacterial materials in proportion, improve the antibacterial activity of compound bacteria inhibition tablet, the growth and breeding of mould and pathogen can effectively be inhibited, securely and reliably, advantage is created for wheat storage.
Description
Technical field
The present invention relates to View of Grain Storage Technoiogy fields, and in particular to a kind of wheat stores the preparation method of compound bacteria inhibition tablet.
Background technique
Wheat is the second largest crops in China for being only second to rice, and wheat is mature, harvest is in summer, and positive value pest is numerous
It educates, stage of development, vulnerable insect infection, since wheat itself hygroscopicity is strong, storage is more difficult, and wheat is being store currently on the market
It when hiding, is just put in storage after only drying mostly, is easy to be influenced by insect pest, caused wheat rotten, bring larger economic loss.
Traditional wheat storage method is that toxic chemical is added into wheat to prevent wheat is mouldy from damaging by worms, general
Insect-proof agent has aluminum phosphate, hydrogen phosphide, fenoxycarb etc., and application method is substantially the same, and is mostly to be wrapped medicament using gauze, by thin
The mediums such as bamboo pole are inserted into bulk grain tank.However, though the mildew and insect proof moth effect of these toxic chemicals is good, if operation is not
It is contaminated when can lead to grain, or even cause operator's poisoning, be detrimental to health, therefore find it is a kind of using comparatively safe and
It is imperative that mildew and insect proof eats into the good storage method of effect.
Summary of the invention
Technical problem to be solved by the present invention lies in the preparation methods for providing a kind of wheat and storing compound bacteria inhibition tablet, are used for
Preferable storage wheat, promotes storage quality.The following technical solution is employed for the technical problems to be solved by the invention to realize:
A kind of wheat stores the preparation method of compound bacteria inhibition tablet, comprising the following steps:
(1) preparation of modified tea polyphenol extract powder:
A. select it is fresh, without discoloration, without the tealeaves of insect pest, freezing processing is carried out to it with liquid nitrogen, is carried out immediately after processing
It crushes, obtains tealeaves crushed material.
Freezing processing is to freeze 30-40min under the conditions of -15 to -5 DEG C in the step a, and then crushing is that 100 mesh are thin
The tealeaves crushed material at end.
B. the food grade ethyl alcohol that mass fraction is 50-60% is added in tealeaves crushed material and impregnates 30-40min, then in 40-
Ultrasonic extraction under conditions of 80 DEG C, obtains extracting solution.
The molar ratio of tealeaves crushed material and food grade ethyl alcohol is 1:10-15 in the step b；Ultrasonic power 60-80W,
Sonication treatment time 20-60min.
C. natrium malicum solution is added into extracting solution, adjusts pH to 7-8, stands, is then filtered by vacuum, is concentrated by evaporation,
Obtain crude extract.
D. 3 times of volumes are added in crude extract, temperature is in 70-90 DEG C of water, while a certain amount of sodium pyrophosphate is added
And calgon, high-speed stirred 5-30min carries out homogeneous, then places it in and carry out pulse magnetic field treatment in pulsed magnetic field instrument,
Filtering, obtains smart purification.
The additive amount of sodium pyrophosphate and calgon is respectively the 2- of crude extract quality in step d in the step d
4%, 1-3%.
When the pulse magnetic field treatment, treatment temperature is 10-25 DEG C, pulse strength 1.0-3.0T, umber of pulse 10-
50。
E. finally smart purification is spray-dried, obtains modified tea polyphenol extract powder.
(2) preparation of compound bacteria inhibition tablet: being put into grind into powder on grinder for dried orange peel, is added modified obtained by step (1)
Tea polyphenol extract object powder, is sufficiently mixed with malic acid and sterile distilled water, adds wormwood extract, spray-dried, so
Compression moulding afterwards obtains compound bacteria inhibition tablet；
The wormwood extract the preparation method comprises the following steps: wormwood clear water is rinsed, dry, is sliced, cooled down again after boiling, repeatedly
Boiling, cooling 6-8 times, then sealed soak 24-36h in 42 degree of white wine is put into, filtrate obtained by filtering removal solid content is
For wormwood extract.
The spray drying step temperature controls 60-80 DEG C, time 60-120min.
The ingredient of the compound bacteria inhibition tablet, as mass fraction, dried orange peel 0.5-2.0%, modified tea polyphenol extract powder
33.0-45.0%, malic acid 1.5-2.5%, wormwood extract 1.5-4.0%, surplus are sterile distilled water.
Described every specification of compound bacteria inhibition tablet is 3-5g, is packed and is invested in small wheat bin with cloth medicine bag, throw-in density 6-
10g/m3。
The beneficial effects of the present invention are:
1, the present invention is before tea polyphenol extract first with liquid nitrogen to the processing of tealeaves quick freeze, it is ensured that extracts, preparation obtains
The tealeaves obtained has good color and organoleptic attribute, remains its effective component as far as possible.
2, the modified tea polyphenol extract that the present invention is prepared, effectively remains the antibacterial effect of tea polyphenols, prepares
The bacteriostasis property of obtained compound bacteria inhibition tablet further increases.
3, pulsed magnetic field technology have action temperature and, break born of the same parents it is efficient, it is without side-effects the features such as, the present invention passes through pulsed magnetic field
Technology extracts the main component of tealeaves, reduces chemical extraction step, can effectively improve extraction efficiency, and process safety
It is good.
4, the present invention mixes tea polyphenol extract object with a variety of natural safe antibacterial substances in proportion, improves compound antibacterial
The antibacterial activity of piece, can effectively inhibit the growth and breeding of mould and pathogen, and easy to use, good antimicrobial effect is safe and non-toxic；
It avoids in traditional fumigation process using the residual of the toxic chemicals substances such as hydrogen phosphide；This production process is simple, condition temperature
With, extracting cycle is shorter, have preferable market application prospect.
5, the present invention utilizes the dipping principle of vacuum tumbler, is collided, is beaten by the forced circulation inside equipment, is quiet
It sets, accelerates distribution and the uniformly penetrating of the active constituent of wormwood extract, improve the distribution of dried orange peel plant fiber, improve and change
The dispersibility of property tea polyphenol extract object；By tumbling technique, avoid and improve prepare compound bacteria inhibition tablet easy to knot groups, drug effect distribution
Uneven processing disadvantages are conducive to drug effect and discharge, improve drug effect so that granulation is carefully and neatly done.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1
(1) preparation of modified tea polyphenol extract powder:
A. select it is fresh, without discoloration, without the tealeaves of insect pest, freezing processing is carried out to it with liquid nitrogen, is freezed under the conditions of -15 DEG C
30min crushes the tealeaves crushed material for 100 mesh smalls immediately after processing.
B. in tealeaves crushed material be added mass fraction be 50% food grade ethyl alcohol impregnate 30min, tealeaves crushed material with
The molar ratio of food grade ethyl alcohol is 1:10, then ultrasonic extraction under conditions of 40 DEG C, ultrasonic power 60W, sonication treatment time
20min obtains extracting solution.
C. natrium malicum solution is added into extracting solution, adjusts pH to 7, stands, is then filtered by vacuum, is concentrated by evaporation, obtains
Crude extract.
D. 3 times of volumes are added in crude extract, temperature is in 70 DEG C of water, while the coke of crude extract quality 2% is added
Sodium phosphate and 1% calgon, high-speed stirred 5min carries out homogeneous, then places it in and carry out pulsed magnetic in pulsed magnetic field instrument
Field processing, treatment temperature are 10 DEG C, pulse strength 1.0T, and umber of pulse 10, filtering obtains smart purification.
E. finally smart purification is spray-dried, temperature controls 60 DEG C, and time 60min obtains modified tea polyphenol and mentions
Take object powder.
(2) preparation of compound bacteria inhibition tablet: being put into grind into powder on grinder for dried orange peel, is added modified obtained by step (1)
Tea polyphenol extract object powder, is sufficiently mixed with malic acid and sterile distilled water, adds wormwood extract, spray-dried, temperature
60 DEG C of degree control, time 60min is then pressed into type, obtains compound bacteria inhibition tablet；
Wherein, wormwood extract the preparation method comprises the following steps: wormwood clear water is rinsed, dry, is sliced, cooled down again after boiling, instead
Boiling, cooling 6 times again, then put into sealed soak in 42 degree of white wine and for 24 hours, filter the as Chinese mugwort of filtrate obtained by removal solid content
Careless extract.
Wherein, the ingredient of compound bacteria inhibition tablet, as mass fraction, dried orange peel 0.5%, modified tea polyphenol extract powder
33.0%, malic acid 1.5%, wormwood extract 1.5%, surplus is sterile distilled water.Every specification of compound bacteria inhibition tablet is 3g, is used
Cloth medicine bag packaging is invested in small wheat bin, throw-in density 6g/m3.
Embodiment 2
(1) preparation of modified tea polyphenol extract powder:
A. select it is fresh, without discoloration, without the tealeaves of insect pest, freezing processing is carried out to it with liquid nitrogen, freezing processing is -5
40min is freezed under the conditions of DEG C, carries out crushing the tealeaves crushed material for 100 mesh smalls immediately after processing.
B. in tealeaves crushed material be added mass fraction be 60% food grade ethyl alcohol impregnate 40min, tealeaves crushed material with
The molar ratio of food grade ethyl alcohol is 1:15；Ultrasonic extraction, ultrasonic power 80W, sonication treatment time under conditions of 80 DEG C again
60min obtains extracting solution.
C. natrium malicum solution is added into extracting solution, adjusts pH to 8, stands, is then filtered by vacuum, is concentrated by evaporation, obtains
Crude extract.
D. 3 times of volumes are added, in water at a temperature of 90 °C in crude extract, while the coke of crude extract quality 4% is added
Sodium phosphate and 3% calgon, high-speed stirred 30min carries out homogeneous, then places it in and carry out pulse in pulsed magnetic field instrument
Magnetic field processing, treatment temperature are 25 DEG C, pulse strength 3.0T, and umber of pulse 50, filtering obtains smart purification.
E. finally smart purification is spray-dried, temperature controls 80 DEG C, and time 120min obtains modified tea polyphenol and mentions
Take object powder.
(2) preparation of compound bacteria inhibition tablet: being put into grind into powder on grinder for dried orange peel, is added modified obtained by step (1)
Tea polyphenol extract object powder, is sufficiently mixed with malic acid and sterile distilled water, adds wormwood extract, spray-dried, temperature
80 DEG C of degree control, time 120min is then pressed into type, obtains compound bacteria inhibition tablet；
Wherein, wormwood extract the preparation method comprises the following steps: wormwood clear water is rinsed, dry, is sliced, cooled down again after boiling, instead
Multiple boiling, cooling 8 times, then sealed soak 36h in 42 degree of white wine is put into, filtrate obtained by filtering removal solid content is Chinese mugwort
Careless extract.
Wherein, the ingredient of compound bacteria inhibition tablet, as mass fraction, dried orange peel 2.0%, modified tea polyphenol extract powder
45.0%, malic acid 2.5%, wormwood extract 4.0%, surplus is sterile distilled water.Every specification of compound bacteria inhibition tablet is 5g, is used
Cloth medicine bag packaging is invested in small wheat bin, throw-in density 10g/m3。
Embodiment 3
(1) preparation of modified tea polyphenol extract powder:
A. select it is fresh, without discoloration, without the tealeaves of insect pest, freezing processing is carried out to it with liquid nitrogen, freezing processing is -10
35min is freezed under the conditions of DEG C, carries out crushing the tealeaves crushed material for 100 mesh smalls immediately after processing.
B. in tealeaves crushed material be added mass fraction be 55% food grade ethyl alcohol impregnate 33min, tealeaves crushed material with
The molar ratio of food grade ethyl alcohol is 1:12, then ultrasonic extraction under conditions of 60 DEG C, ultrasonic power 70W, sonication treatment time
30min obtains extracting solution.
C. natrium malicum solution is added into extracting solution, adjusts pH to 7, stands, is then filtered by vacuum, is concentrated by evaporation, obtains
Crude extract.
D. 3 times of volumes are added in crude extract, temperature is in 80 DEG C of water, while the coke of crude extract quality 3% is added
Sodium phosphate and 2% calgon, high-speed stirred 10min carries out homogeneous, then places it in and carry out pulse in pulsed magnetic field instrument
Magnetic field processing, treatment temperature are 15 DEG C, pulse strength 2.0T, and umber of pulse 20, filtering obtains smart purification.
E. finally smart purification is spray-dried, temperature controls 70 DEG C, and time 80min obtains modified tea polyphenol and mentions
Take object powder.
(2) preparation of compound bacteria inhibition tablet: being put into grind into powder on grinder for dried orange peel, is added modified obtained by step (1)
Tea polyphenol extract object powder, is sufficiently mixed with malic acid and sterile distilled water, adds wormwood extract, spray-dried, temperature
70 DEG C of degree control, time 80min is then pressed into type, obtains compound bacteria inhibition tablet；
Wherein, wormwood extract the preparation method comprises the following steps: wormwood clear water is rinsed, dry, is sliced, cooled down again after boiling, instead
Multiple boiling, cooling 7 times, then sealed soak 26h in 42 degree of white wine is put into, filtrate obtained by filtering removal solid content is Chinese mugwort
Careless extract.
Wherein, the ingredient of compound bacteria inhibition tablet, as mass fraction, dried orange peel 1.0%, modified tea polyphenol extract powder
35.0%, malic acid 2.0%, wormwood extract 3.0%, surplus is sterile distilled water.Every specification of compound bacteria inhibition tablet is 4g, is used
Cloth medicine bag packaging is invested in small wheat bin, throw-in density 8g/m3。
Embodiment 4
The present embodiment content and 1 content of embodiment are essentially identical, and something in common no longer repeats, the difference is that: it is compound
In the preparation process of bacteria inhibition tablet: dried orange peel being put into grind into powder on grinder, modified tea polyphenol obtained by step (1) is added and extracts
Object powder, is sufficiently mixed with malic acid and sterile distilled water, adds wormwood extract；Gained mixed material is sent into vacuum rolling
Rub machine, control tumbling 0.5h stops 1h, then tumbling 2h stops 1h, tumbling speed control 12r/min, vacuum control 71kPa in tank, temperature
3 DEG C of control, finally spray-dried again, temperature controls 60 DEG C, and time 60min is then pressed into type, obtains compound bacteria inhibition tablet.
Remaining step method and treatment conditions are same as Example 1.
Comparative example
(1) preparation of tea polyphenol extract object powder:
A. select it is fresh, without discoloration, without the tealeaves of insect pest, freezing processing is carried out to it with liquid nitrogen, is freezed under the conditions of -15 DEG C
30min crushes the tealeaves crushed material for 100 mesh smalls immediately after processing.
B. ultrasonic extraction under conditions of tealeaves crushed material is placed at 40 DEG C, ultrasonic power 60W, sonication treatment time
20min obtains extracting solution.
C. extracting solution is spray-dried, temperature controls 60 DEG C, time 60min, obtains tea polyphenol extract object powder.
(2) preparation of compound bacteria inhibition tablet: dried orange peel is put into grind into powder on grinder, it is more that tea obtained by step (1) is added
Phenol extraction object powder, is sufficiently mixed with malic acid and sterile distilled water, adds wormwood extract, spray-dried, temperature control
60 DEG C of system, time 60min is then pressed into type, obtains compound bacteria inhibition tablet；
Wherein, wormwood extract the preparation method comprises the following steps: wormwood clear water is rinsed, dry, is sliced, cooled down again after boiling, instead
Boiling, cooling 6 times again, then put into sealed soak in 42 degree of white wine and for 24 hours, filter the as Chinese mugwort of filtrate obtained by removal solid content
Careless extract.
Wherein, the ingredient of compound bacteria inhibition tablet, as mass fraction, dried orange peel 0.5%, tea polyphenol extract object powder 33.0%,
Malic acid 1.5%, wormwood extract 1.5%, surplus are sterile distilled water.Every specification of compound bacteria inhibition tablet is 3g, with cloth medicine bag
Packaging is invested in small wheat bin, throw-in density 6g/m3。
Test method
Wheat seed is acted on using compound bacteria inhibition tablet and its antibacterial and bactericidal effect is compared；
(1) experimental strain
Aspergillus glaucus；
(2) experimental method
Using the method for plate culture count, it is simulation strain with Aspergillus glaucus, measures compound bacteria inhibition tablet in wheat storage period
Application.
Wheat seed 5kg in extracting bin, manual humidification make it contain 13.5% moisture, are put into made of galvanized iron sheet
It is stored in sterile chamber, by electric heater and ultrasonic humidifier manual simulation and sets constant temperature and humidity, initially
Set temperature is 29 DEG C, humidity 73.4%；It takes 250g wheat seed to divide equally and is transferred in 25 × 200mL test tube；Detection storage
The situation of change of Aspergillus glaucus in the process:
The concentration that the compound bacteria inhibition tablet that embodiment, comparative example are prepared respectively is respectively 0.75% with final mass score
It is added in test tube, and accesses the Aspergillus glaucus of culture to logarithmic phase, initial concentration 103CFU/mL, not add compound suppression
The test tube of bacterium piece sample is control group, is uniformly mixed, is placed on 37 DEG C of shaking table culture.Water is controlled during culture in sterile chamber
Point with daily 0.2 DEG C of speed increase, in 0h, for 24 hours, when 48h, 72h, test tube taking out, dilution spread is on NA culture medium step by step,
Measure remaining bacterium number.Measuring method according to " GB4789.2-2016 " food hygiene microbiologic inhibition tests total plate count method into
Row.
(3) experimental result
As seen from the experiment, after cultivating 72h, the Aspergillus glaucus of blank control group mushrooms out breeding, by initial concentration
Rise to 6.83Log CFU/mL.After same culture 72h, the growth of the Aspergillus glaucus handled by comparative example receives certain
Inhibiting effect, total plate count are slowly increased to 2.82Log CFU/mL.In the wheat samples of compound bacteria inhibition tablet processing, grayish green song
Mould breeding has obtained certain inhibition, and remaining bacterium number is 1.16Log CFU/mL hereinafter, sterilizing rate works well；Embodiment 4
It is middle that compound bacteria inhibition tablet is prepared using vacuum tumbling technique, show more obvious antibacterial action.Therefore, in actual use
This compound bacteria inhibition tablet can effectively inhibit bacterial reproduction, ensure the storage safety of wheat seed.
Specific effect is shown in Table 1:
1 different type processing method of table is antibacterial, bactericidal effect compares
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (9)
1. the preparation method that a kind of wheat stores compound bacteria inhibition tablet, it is characterised in that: the following steps are included:
(1) preparation of modified tea polyphenol extract powder:
A. select it is fresh, without discoloration, without the tealeaves of insect pest, freezing processing is carried out to it with liquid nitrogen, is crushed immediately after processing,
Obtain tealeaves crushed material；
B. the food grade ethyl alcohol that mass fraction is 50-60% is added in tealeaves crushed material and impregnates 30-40min, then at 40-80 DEG C
Under conditions of ultrasonic extraction, obtain extracting solution；
C. natrium malicum solution is added into extracting solution, adjusts pH to 7-8, stands, is then filtered by vacuum, is concentrated by evaporation, obtains slightly
Extract；
D. 3 times of volumes are added in crude extract, temperature is in 70-90 DEG C of water, while a certain amount of sodium pyrophosphate and six are added
Sodium metaphosphate, high-speed stirred 5-30min carries out homogeneous, then places it in and carry out pulse magnetic field treatment in pulsed magnetic field instrument, filters,
Obtain smart purification；
E. finally smart purification is spray-dried, obtains modified tea polyphenol extract powder；
(2) preparation of compound bacteria inhibition tablet: being put into grind into powder on grinder for dried orange peel, and it is more that modified tea obtained by step (1) is added
Phenol extraction object powder, is sufficiently mixed with malic acid and sterile distilled water, adds wormwood extract, spray-dried, then presses
Type is made, obtains compound bacteria inhibition tablet.
2. the preparation method that wheat according to claim 1 stores compound bacteria inhibition tablet, it is characterised in that: the wormwood is extracted
Object the preparation method comprises the following steps: wormwood clear water is rinsed, dry, is sliced, cooled down again after boiling, repeatedly boiling, 6-8 time cooling, then general
It puts into sealed soak 24-36h in 42 degree of white wine, and filtrate obtained by filtering removal solid content is wormwood extract.
3. the preparation method that wheat according to claim 1 stores compound bacteria inhibition tablet, it is characterised in that: in the step a
Freezing processing is to freeze 30-40min under the conditions of -15 to -5 DEG C, then crushes the tealeaves crushed material for 100 mesh smalls.
4. the preparation method that wheat according to claim 1 stores compound bacteria inhibition tablet, it is characterised in that: in the step b
The molar ratio of tealeaves crushed material and food grade ethyl alcohol is 1:10-15；Ultrasonic power 60-80W, sonication treatment time 20-
60min。
5. the preparation method that wheat according to claim 1 stores compound bacteria inhibition tablet, it is characterised in that: in the step d
The additive amount of sodium pyrophosphate and calgon is respectively 2-4%, 1-3% of crude extract quality.
6. the preparation method that wheat according to claim 1 stores compound bacteria inhibition tablet, it is characterised in that: the pulsed magnetic field
When processing, treatment temperature is 10-25 DEG C, pulse strength 1.0-3.0T, umber of pulse 10-50.
7. the preparation method that wheat according to claim 1 stores compound bacteria inhibition tablet, it is characterised in that: the spray drying
Step temperature controls 60-80 DEG C, time 60-120min.
8. the preparation method that wheat according to claim 1 stores compound bacteria inhibition tablet, it is characterised in that: described compound antibacterial
The ingredient of piece, as mass fraction, dried orange peel 0.5-2.0%, modified tea polyphenol extract powder 33.0-45.0%, malic acid
1.5-2.5%, wormwood extract 1.5-4.0%, surplus are sterile distilled water.
9. the preparation method that wheat according to claim 1 stores compound bacteria inhibition tablet, it is characterised in that: described compound antibacterial
Every specification of piece is 3-5g, is packed and is invested in small wheat bin with cloth medicine bag, throw-in density 6-10g/m3。
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| CN114133747A (en) * | 2021-12-31 | 2022-03-04 | 浙江恒诚鞋业有限公司 | Antibacterial insole and preparation method thereof |
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