CN110031411A - A kind of spectrum imaging system - Google Patents
A kind of spectrum imaging system Download PDFInfo
- Publication number
- CN110031411A CN110031411A CN201910308760.1A CN201910308760A CN110031411A CN 110031411 A CN110031411 A CN 110031411A CN 201910308760 A CN201910308760 A CN 201910308760A CN 110031411 A CN110031411 A CN 110031411A
- Authority
- CN
- China
- Prior art keywords
- area
- ccd
- imaging system
- infrared
- reception device
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000003384 imaging method Methods 0.000 title claims abstract description 23
- 238000001228 spectrum Methods 0.000 title claims abstract description 22
- 230000003287 optical effect Effects 0.000 claims abstract description 17
- 238000001514 detection method Methods 0.000 claims abstract description 12
- 230000003595 spectral effect Effects 0.000 claims description 7
- 229910052738 indium Inorganic materials 0.000 claims description 3
- APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 claims description 3
- JBRZTFJDHDCESZ-UHFFFAOYSA-N AsGa Chemical compound [As]#[Ga] JBRZTFJDHDCESZ-UHFFFAOYSA-N 0.000 claims description 2
- 238000000799 fluorescence microscopy Methods 0.000 abstract description 5
- 239000000523 sample Substances 0.000 abstract description 3
- 238000012544 monitoring process Methods 0.000 abstract 1
- 238000002381 microspectrum Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000000149 penetrating effect Effects 0.000 description 2
- GYHNNYVSQQEPJS-UHFFFAOYSA-N Gallium Chemical compound [Ga] GYHNNYVSQQEPJS-UHFFFAOYSA-N 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 229910052733 gallium Inorganic materials 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
Abstract
The invention discloses a kind of spectrum imaging systems, including laser, fluorescence inverted microscope, monochromator device and optical detection CCD reception device；The laser light source is accessed in fluorescence inverted microscope optical path by beam expander；The monochromator device is set to the outside of the fluorescence inverted microscope and is connected to its chassis interior, and is circumscribed with optical detection CCD reception device.The acquisition of microcell real time spectrum and the fluorescence imaging of I area of Visible-to-Near InfaRed-near-infrared, II area's wave band can be achieved at the same time in package unit, has important value to the microscopic spectrum variation monitoring of type probe living in biological tissue, has great importance in field of biomedicine.
Description
Technical field
The present invention relates to a kind of spectrum imaging system more particularly to a kind of I area of Visible-to-Near InfaRed-near-infrared, IIth area are micro-
Spectrum imaging system.
Background technique
Imaging-PAM is widely used in biomedical imaging field and biological living imaging field at present.Fluorescence at
The work spectral range of picture is mainly I area 650-950nm of visible light 400-650nm and near-infrared.Relative to general visible and
I area's Imaging-PAM of near-infrared, II area's fluorescence imaging excitation wavelength of near-infrared and launch wavelength 1000-1700nm are longer, can
It significantly reduces light and is penetrating the scattering phenomenon in biological tissue, in addition its photon autologous tissue absorbs less, cause autofluorescence effect
The features such as low, can penetrate unprecedented deep tissue in vivo and possess high spatial resolution in fluorescence imaging.It is thus close
Infrared IIth area Imaging-PAM shows great potential in biological living imaging field, it has also become international research living body fluorescent at
The newest hot spot direction of picture.There has been II area's imaging device of near-infrared in the country at present, but without corresponding spectra collection system
System.
Summary of the invention
Goal of the invention: it is an object of the present invention to provide a kind of imagings of I area of Visible-to-Near InfaRed-near-infrared, II area's microspectrum to be
Microscopic spectrum acquisition and the fluorescence imaging of I area of Visible-to-Near InfaRed-near-infrared, II area's wave band can be achieved at the same time in system, the system.
Technical solution: a kind of spectrum imaging system of the present invention, including laser, fluorescence inverted microscope, monochrome
Instrument device and optical detection CCD reception device, the light source of the laser access the light of fluorescence inverted microscope by beam expander
Lu Zhong；The monochromator device is set to the outside of fluorescence inverted microscope and is connected to its chassis interior, and is circumscribed with optics
Detect CCD reception device.
Further, the wavelength of the laser light source is 300-1200nm, power 0.1-50W.
Further, the monochromator device includes entrance slit, quasi-optical mirror, grating, reflecting mirror.
Further, all camera lenses and the passable wave-length coverage of reflecting mirror in the fluorescence inverted microscope entirety optical path
For 300-2600nm.
Further, the optical detection CCD reception device includes I area's CCD reception device of visible light-near-infrared and close red
Outer IIth area CCD reception device, the I area CCD of visible light-near-infrared can survey spectral region 400-1050nm, II area CCD of near-infrared
Spectral region 900-1700nm can be surveyed.
Package unit can realize microscopic spectrum acquisition and the micro-imaging of I area of Visible-to-Near InfaRed-near-infrared, II area's wave band.
The utility model has the advantages that II area's microspectrum imaging system of a kind of I area of Visible-to-Near InfaRed-near-infrared of the invention is outside
Monochromator equipment, utilization and optical detection CCD reception device are connect, the microscopic spectrum of visible light and near infrared band can be achieved at the same time
Acquisition and fluorescence imaging, are capable of providing the real time spectrum of biological microcell, especially provide more type probe research living in biological tissue
Good Research foundation, has great importance in field of biomedicine.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention；
Fig. 2 is structure top view of the invention；
Fig. 3 is the structural schematic diagram of monochromator device of the present invention.
Specific embodiment
Further description of the technical solution of the present invention with reference to the accompanying drawings and examples.
Referring to Fig. 1 and Fig. 2, a kind of I area of Visible-to-Near InfaRed-near-infrared, II area's microspectrum imaging system of the present invention includes
Laser 1, fluorescence inverted microscope 2, monochromator device 3 and optical detection CCD reception device 4；The light source of the laser 1 is logical
Beam expander 5 is crossed to access in the optical path of fluorescence inverted microscope 2；The monochromator device 3 is set to the outer of fluorescence inverted microscope 2
Side is simultaneously connected to its chassis interior, and is circumscribed with optical detection CCD reception device 4.
The wavelength of 1 light source of laser is 300-1200nm, power 0.1-50W.
The monochromator device 3 includes entrance slit S1, quasi-optical mirror M1 and M4, grating G, reflecting mirror M2 and M3.
Package unit can realize microscopic spectrum acquisition and the micro-imaging of I area of Visible-to-Near InfaRed-near-infrared, II area's wave band.
Fig. 3 is the structural schematic diagram of monochromator device 3, its working principle is that after light beam passes through entrance slit S1, by standard
Light microscopic M1, reflecting mirror M2 are irradiated on grating G, are scattered light by grating G and are reached reflecting mirror M3, then is anti-through reflecting mirror M3
Outlet is reached by quasi-optical mirror M4 after penetrating, is received by optical detection CCD reception device 4.
The optical detection CCD reception device 4 includes I area's CCD reception device of visible light-near-infrared and IIth area of near-infrared
CCD reception device；The I area CCD of visible light-near-infrared is EM-CCD, can survey spectral region 400-1050nm；The near-infrared
II area CCD is indium gallium arsenic CCD, can survey spectral region 900-1700nm.
The present invention is utilized respectively EM-CCD and indium gallium by the external monochromator device 3 of pedestal in fluorescence inverted microscope 2
Arsenic CCD, while microscopic spectrum acquisition and the micro-imaging of II area's wave band of I area's wave band of visible light-near-infrared and near-infrared are realized,
It is capable of providing the real time spectrum of biological microcell, especially provides better Research foundation to type probe research living.
Claims (7)
1. a kind of spectrum imaging system, it is characterised in that: including laser (1), fluorescence inverted microscope (2), monochromator device
(3) and optical detection CCD reception device (4)；The light source of the laser (1) is inverted micro- by beam expander (5) access fluorescence
In the optical path of mirror (2)；The monochromator device (3) is set to the outside of fluorescence inverted microscope (2) and connects with its chassis interior
It is logical, and it is circumscribed with optical detection CCD reception device (4).
2. spectrum imaging system according to claim 1, it is characterised in that the wavelength of laser (1) light source is 300-
1200nm, power 0.1-50W.
3. spectrum imaging system according to claim 1, it is characterised in that the whole optical path of the fluorescence inverted microscope (2)
In all camera lenses and the passable wave-length coverage of reflecting mirror be 300-2600nm.
4. spectrum imaging system according to claim 1, it is characterised in that the monochromator device (3) includes entrance slit
(S1), quasi-optical mirror (M1) and (M4), grating (G), reflecting mirror (M2) and (M3).
5. spectrum imaging system according to claim 1, it is characterised in that optical detection CCD reception device (4) packet
Include II area's CCD reception device of I area's CCD reception device of visible light-near-infrared and near-infrared.
6. spectrum imaging system according to claim 5, it is characterised in that the I area CCD of visible light-near-infrared is EM-
CCD can survey spectral region 400-1050nm.
7. spectrum imaging system according to claim 5, it is characterised in that the II area CCD of near-infrared is indium gallium arsenic CCD,
Spectral region 900-1700nm can be surveyed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910308760.1A CN110031411A (en) | 2019-04-17 | 2019-04-17 | A kind of spectrum imaging system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910308760.1A CN110031411A (en) | 2019-04-17 | 2019-04-17 | A kind of spectrum imaging system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110031411A true CN110031411A (en) | 2019-07-19 |
Family
ID=67238806
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910308760.1A Pending CN110031411A (en) | 2019-04-17 | 2019-04-17 | A kind of spectrum imaging system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110031411A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113866192A (en) * | 2021-09-27 | 2021-12-31 | 中国科学院高能物理研究所 | Microscopic imaging method and system based on transparent scintillator film |
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Application publication date: 20190719 |