CN107411707A - A kind of tumor-microvessel imager and tumor-microvessel imaging method - Google Patents
A kind of tumor-microvessel imager and tumor-microvessel imaging method Download PDFInfo
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- CN107411707A CN107411707A CN201710318992.6A CN201710318992A CN107411707A CN 107411707 A CN107411707 A CN 107411707A CN 201710318992 A CN201710318992 A CN 201710318992A CN 107411707 A CN107411707 A CN 107411707A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0062—Arrangements for scanning
- A61B5/0064—Body surface scanning
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0073—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by tomography, i.e. reconstruction of 3D images from 2D projections
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0077—Devices for viewing the surface of the body, e.g. camera, magnifying lens
Abstract
The invention discloses a kind of tumor-microvessel imager and tumor-microvessel imaging method, tumor-microvessel imager includes infrared co-focusing imaging part and control section；Near-infrared co-focusing imaging part includes near infrared laser, dichroscope, two-dimensional scanning mirrors, scanning lens group, image-forming objective lens, near-infrared fluorescent optical filter, convergent lens, pin hole and detector；Scanning lens group by field lens and cylinder microscope group into；Control section includes being used for two-dimensional scan control module, signal amplification and acquisition module and the data processing image display for controlling two-dimensional scanning mirrors；Fluorescence excitation spectrum is injected in the blood vessel of organism to be seen in 1000 to 1350nm and near-infrared quantum dots of the fluorescence emission spectrum between 1350nm to 1500nm, then the tumor section of the organism is placed under tumor-microvessel imager and is observed.Of the invention can realize to high-resolution carries out Complete three-dimensional imaging to organism tumor-microvessel.
Description
Technical field
The invention belongs to field of biomedicine technology, is related to a kind of tumor-microvessel imager and tumor-microvessel imaging side
Method, more particularly to a kind of operating wavelength range is in the Image-forming instrument and tumor-microvessel imaging method of 1300 ~ 1500nm wave bands.
Background technology
Growth of the generation of tumor-microvessel to tumour and intrusion transfer, particularly have to tumour early stage it is extremely important
Influence, research shows that the fast-growth of tumour especially malignant tumour relies on angiogenesis.Capilary is rich in some tumours
Richness, form is inmature, the complicated network structure, exists in composition, form and spatial distribution etc. obvious heterogeneous that is, swollen
Knurl microvascular architecture phenotype heterogeneity.This heterogeneity may be derived from various blood vessel lifes in the regulatory mechanism of Tumor Angiongesis
Into the unbalance of promotive factor and inhibiting factor.Between precancerous lesion and primary tumor and metastatic tumor, microvascular architecture phenotype is also deposited
In difference, the therapeutic process of another aspect cancer therapy drug also has a significant effect to microvascular architecture,
Therefore, if can long time-histories observation vivo tumor region capilary three-D space structure change, by tumour
Occurrence and development process provides more fully objective basis, and new thinking is provided for tumour antiangiogenesis therapy
Because capilary is distributed in tumor tissues, its complete structure can be in several millimeters of magnitudes, and more trickle capillary pipeline
About some tens of pm or so, this makes the research of the three-D space structure of capilary lack effective tool.Clinic is for blood vessel at present
Imaging method mainly have ultrasound, CT angiograms and nuclear-magnetism angiogram, but these methods want to differentiate some tens of pm
Capillary pipeline is still extremely difficult.
Gradually starting to move towards the optical imaging method of clinical practice at present has the resolution ratio of micron even submicron order,
Capilary can be easily differentiated, but the scattering of biological tissue seriously limits the imaging depth of optical imaging method, even
The optical coherent chromatographic imaging and multi-photon imaging, the imaging depth reported at present for having certain depth imaging capability also can only be
1 millimeter.In recent years, the area's fluorescent marker of near-infrared two that Stanford University Dai et al. made has reached preferable depth
Imaging effect, the possibility for the vivo observation of tumor blood vessels intact form provide theories integration.
The content of the invention
In order to solve the above-mentioned technical problem, the invention provides a kind of tumor-microvessel Image-forming instrument and tumor-microvessel into
Image space method, it can carry out profound imaging to tumor vessel, draw out the three-dimensional space shape of tumor-microvessel.
Technical scheme is used by the tumor-microvessel imager of the present invention：A kind of tumor-microvessel imager, it is special
Sign is：Including infrared co-focusing imaging part and control section；Swash including near-infrared the near-infrared co-focusing imaging part
Light device, dichroscope, two-dimensional scanning mirrors, scanning lens group, image-forming objective lens, near-infrared fluorescent optical filter, convergent lens, pin hole
And detector；The scanning lens group by field lens and cylinder microscope group into；
The control section includes being used for two-dimensional scan control module, signal amplification and the collection for controlling the two-dimensional scanning mirrors
Module and data processing image display；
The near infrared light that the near infrared laser is sent is after the dichroscope is reflected into the two-dimensional scanning mirrors
Into the field lens, the plane of scanning motion is formed at told field lens focal plane, incides the imaging by the cylinder mirror is parallel afterwards
Object lens simultaneously assemble the plane of scanning motion to form sample plane in sample stage, scan biological specimen；The near-infrared fluorescent that sample is excited
It is parallel after being collected by the image-forming objective lens to be changed into directional light by the field lens after cylinder mirror convergence, then by described two
The dichroscope is passed through in projection after dimension scanning galvanometer moves back scanning, by the near-infrared fluorescent optical filter, then by the convergence
Lens focus is a bit, the point enters the detector after passing through the pin hole；The signal of detector output is put through the signal
Greatly signal transacting is carried out with being sent into the data processing image display after acquisition module.
The tumor-microvessel imaging method of the present invention the technical scheme adopted is that a kind of tumor-microvessel imaging method,
It is characterized in that：The signal that point by point scanning detects is converted into the gray value of single pixel by data processing image display, will
Single layer image is obtained after the arrangement of its order, can so obtain the individual layer sectional drawing of tumor-microvessel.
The tumor-microvessel imaging method of the present invention the technical scheme adopted is that a kind of tumor-microvessel imaging method,
It is characterized in that：The signal that point by point scanning detects is converted into the gray value of single pixel by data processing image display, will
Single layer image is obtained after the arrangement of its order, then sample stage rises certain altitude, repeats scanned samples and obtains next layer of list
Tomographic image, certain number of plies is so circulated, it is that can obtain the three-dimensional of tumor-microvessel to tie that these sequence images are carried out into three-dimensional reconstruction
Composition picture.
The present invention is based on biological tissue in the area of near-infrared two（1300nm~1500nm）Absorb less, scattering less and autofluorescence
The characteristics of low, with reference to the ability of the tomography of conjugate focus imaging technique, and with near-infrared quantum dots marked tumor blood vessel, so that
Realize and the three-dimensional structure of tumor-microvessel is imaged.Near-infrared quantum dots exciting light and transmitting light be make use of all close to near-infrared two
The characteristics of area, preferably laser wavelength range 1000nm ~ 1350nm near-infrared laser excitation emission spectra in 1300nm ~ 1500nm
The area's quantum dot of near-infrared two, so as to realize tumor vascular deep layer living imaging, obtain the three-dimensional completeness of tumor-microvessel
State.
Brief description of the drawings
Fig. 1 is the structural representation of the embodiment of the present invention；
In figure, 1- near infrared lasers, 2- two-dimensional scan control modules, 3- two-dimensional scanning mirrors, 4- field lenses, 5- cylinder mirrors, 6- into
Picture object lens, 7- sample stages, 8- dichroscopes, 9- near-infrared fluorescent optical filters, 10- convergent lenses, 11- pin holes, 12- detectors,
13- signals amplify and acquisition module, 14- data processing image displays.
Embodiment
In order that the objects, technical solutions and advantages of the present invention become apparent from understanding, it is right below in conjunction with drawings and Examples
The present invention is further elaborated, it should be appreciated that and the specific embodiments described herein are merely illustrative of the present invention, and
Be not used in the restriction present invention, as long as in addition, technical characteristic involved in each embodiment of present invention described below that
Conflict can is not formed between this to be mutually combined.
See Fig. 1, a kind of tumor-microvessel imager provided by the invention, including infrared co-focusing imaging part and control
Part；Near-infrared co-focusing imaging part includes near infrared laser 1, dichroscope 8, two-dimensional scanning mirrors 3, scanning lens
Group, image-forming objective lens 6, near-infrared fluorescent optical filter 9, convergent lens 10, pin hole 11 and detector 12；Scanning lens group is by field lens 4
Formed with cylinder mirror 5；Control section controls the two-dimensional scan control module 2 of two-dimensional scanning mirrors 3, signal to amplify with adopting including being used for
Collect module 13 and data processing image display 14；
The near infrared light that near infrared laser 1 is sent enters field lens 4 after dichroscope 8 is reflected into two-dimensional scanning mirrors 3,
The plane of scanning motion is formed at the told focal plane of field lens 4, image-forming objective lens 6 is incided by cylinder mirror 5 is parallel afterwards and is assembled in sample stage 7
The plane of scanning motion of sample plane is formed, scans biological specimen；The near-infrared fluorescent that sample is excited is put down after being collected by image-forming objective lens 6
Cylinder mirror 5 of passing through by field lens 4 is changed into directional light after assembling, then after two-dimensional scanning mirrors 3 move back scanning projection by two to
Look mirror 8, by near-infrared fluorescent optical filter 9, then it is converged lens 10 and is focused to a bit, the point enters detection after passing through pin hole 11
Device 12；The signal that detector 12 exports amplifies through signal to be carried out with being sent into data processing image display 14 after acquisition module 13
Signal transacting.
In order to obtain good imaging effect, foregoing each optics should have preferable transmitance in designated wavelength range
Or reflectivity, detector have preferable response curve.Specific features are as follows：
The operation wavelength of near infrared laser 1 should be the semiconductor pumped solid of 1000 ~ 1350nm, preferably 1064nm or 1310nm and swash
Light device.
Dichroscope 8 is using the light reflectivity ＞ 90% for being less than 1350nm to wavelength, to wavelength 1350nm ~ 1500nm's
Light transmission rate is more than 90% long pass filter.
Two-dimensional scanning mirrors 3 are more than 90% to wavelength 1000nm ~ 1600nm light reflectivity.
Field lens 4, cylinder mirror 5, image-forming objective lens 6 and convergent lens 10 are more than to wavelength 1000nm ~ 1600nm light transmission rate
90%, the operating distance ＞ 10mm of image-forming objective lens 6, numerical aperture are more than 0.2.
Near-infrared fluorescent optical filter 9 is more than 90% to wavelength 1400nm ~ 1500nm light transmission rate, and to cutoff wavelength OD
Bandpass filter more than 3.
Pin hole 11 should be located at the focal plane of convergent lens 10, and its size is the Airy disc size of convergent lens 10.
Detector 12 should abut pin hole and place, and should use the photomultiplier of indium gallium arsenic material.
The near-infrared quantum dots that fluorescence emission wavelengths are 1300nm ~ 1500nm should be filled in the blood vessel of biological specimen.
A kind of tumor-microvessel imaging method provided by the invention, for carrying out image to the individual layer section of tumor-microvessel
Obtain, its specific implementation process is：
The biological specimen for being marked with the near-infrared quantum dots that fluorescence emission wavelengths are 1300nm ~ 1500nm is placed in the tumour blood
Under pipe imager, it is laggard that near infrared light that near infrared laser 1 is sent by dichroscope 8 is reflected into two-dimensional scanning mirrors 3
Enter field lens 4, the plane of scanning motion is formed at the focal plane of field lens 4, incides image-forming objective lens 6 and in sample stage 7 by cylinder mirror 5 is parallel afterwards
Assemble the plane of scanning motion for forming sample plane.The near-infrared fluorescent that sample is excited is parallel after being collected by image-forming objective lens 6 to pass through cylinder
It is changed into directional light by field lens 4 after the convergence of mirror 5, then dichroscope 8 is passed through in projection after two-dimensional scanning mirrors 3 move back scanning, passes through
Near-infrared fluorescent optical filter 9 is crossed, then is converged lens 10 and is focused to a bit, the point enters detector 12 after passing through pin hole 11；Visit
Survey after the signal that device 12 exports is gathered by signal amplification with acquisition module 13 and input to data processing image display 14, data
The signal that point by point scanning detects is converted into the gray value of single pixel by processing image display 14, after its order is arranged
To single layer image, the individual layer sectional drawing of tumor-microvessel can be so obtained.
A kind of tumor-microvessel imaging method provided by the invention, for being obtained to the three-dimensional structure image of tumor-microvessel
Take, its specific implementation process is：
The biological specimen for being marked with the near-infrared quantum dots that fluorescence emission wavelengths are 1300nm ~ 1500nm is placed in the tumour blood
Under pipe imager, it is laggard that near infrared light that near infrared laser 1 is sent by dichroscope 8 is reflected into two-dimensional scanning mirrors 3
Enter field lens 4, the plane of scanning motion is formed at the focal plane of field lens 4, incides image-forming objective lens 6 and in sample stage 7 by cylinder mirror 5 is parallel afterwards
Assemble the plane of scanning motion for forming sample plane.The near-infrared fluorescent that sample is excited is parallel after being collected by image-forming objective lens 6 to pass through cylinder
It is changed into directional light by field lens 4 after the convergence of mirror 5, then dichroscope 8 is passed through in projection after two-dimensional scanning mirrors 3 move back scanning, passes through
Near-infrared fluorescent optical filter 9 is crossed, then is converged lens 10 and is focused to a bit, the point enters detector 12 after passing through pin hole 11；Visit
Survey after the signal that device 12 exports is gathered by signal amplification with acquisition module 13 and input to data processing image display 14, data
The signal that point by point scanning detects is converted into the gray value of single pixel by processing image display 14, after its order is arranged
To single layer image, then the rising of sample stage 7 certain altitude, preferably 5um, repeats the individual layer that said process can obtain next layer
Image, certain number of plies, preferably 1000 layers are so circulated, these sequence images are carried out into three-dimensional reconstruction can obtain tumor-microvessel
Three-dimensional structure image.
Although this specification has more used near infrared laser 1, two-dimensional scan control module 2, two-dimensional scanning mirrors
3rd, field lens 4, cylinder mirror 5, image-forming objective lens 6, sample stage 7, dichroscope 8, near-infrared fluorescent optical filter 9, convergent lens 10, pin hole 11
With detector 12, signal amplification and the term such as acquisition module 13 and data processing image display 14, but it is not precluded from using
The possibility of other terms.Using these terms just for the sake of more easily describing the essence of the present invention, it is construed as
The additional limitation of any one is all disagreed with spirit of the present invention.
Fluorescence excitation spectrum is arrived into the near-infrared of 1350nm and fluorescence emission spectrum between 1350nm to 1500nm 1000
Quantum dot injects in the blood vessel of organism to be seen, then the tumor section of the organism is placed under tumor-microvessel imager
Row observation.Of the invention can realize to high-resolution carries out Complete three-dimensional imaging to organism tumor-microvessel.
It should be appreciated that the part that this specification does not elaborate belongs to prior art.
It should be appreciated that the above-mentioned description for preferred embodiment is more detailed, therefore can not be considered to this
The limitation of invention patent protection scope, one of ordinary skill in the art are not departing from power of the present invention under the enlightenment of the present invention
Profit is required under protected ambit, can also be made replacement or deformation, be each fallen within protection scope of the present invention, this hair
It is bright scope is claimed to be determined by the appended claims.
Claims (10)
- A kind of 1. tumor-microvessel imager, it is characterised in that：Including infrared co-focusing imaging part and control section；The near-infrared co-focusing imaging part includes near infrared laser（1）, dichroscope（8）, two-dimensional scanning mirrors（3）、 Scanning lens group, image-forming objective lens（6）, near-infrared fluorescent optical filter（9）, convergent lens（10）, pin hole（11）And detector（12）； The scanning lens group is by field lens（4）With cylinder mirror（5）Composition；The control section includes being used to control the two-dimensional scanning mirrors（3）Two-dimensional scan control module（2）, signal amplification With acquisition module（13）With data processing image display（14）；The near infrared laser（1）The near infrared light sent passes through the dichroscope（8）It is reflected into the two-dimensional scan Galvanometer（3）Enter the field lens afterwards（4）, in told field lens（4）The plane of scanning motion is formed at focal plane, afterwards by the cylinder mirror（5） It is parallel to incide the image-forming objective lens（6）And in sample stage（7）The plane of scanning motion for forming sample plane is assembled, scans biological sample This；The near-infrared fluorescent that sample is excited is by the image-forming objective lens（6）It is parallel after collection to pass through the cylinder mirror（5）After convergence by The field lens（4）It is changed into directional light, then by the two-dimensional scanning mirrors（3）The dichroscope is passed through in projection after moving back scanning （8）, by the near-infrared fluorescent optical filter（9）, then by the convergent lens（10）It is focused to a bit, the point passes through the pin Hole（11）Enter the detector afterwards（12）；Detector（12）The signal of output amplifies through the signal and acquisition module（13）Afterwards It is sent into the data processing image display（14）Carry out signal transacting.
- 2. tumor-microvessel imager according to claim 1, it is characterised in that：The near infrared laser（1）Work Wavelength should be 1000 ~ 1350nm.
- 3. tumor-microvessel imager according to claim 1, it is characterised in that：The dichroscope（8）Using to ripple The long light reflectivity ＞ 90% less than 1350nm, to wavelength in the logical optical filtering of length of the 1350nm ~ 1500nm light transmission rate more than 90% Piece.
- 4. tumor-microvessel imager according to claim 1, it is characterised in that：The two-dimensional scanning mirrors（3）To ripple Long 1000nm ~ 1600nm light reflectivity is more than 90%.
- 5. tumor-microvessel imager according to claim 1, it is characterised in that：The field lens（4）, cylinder mirror（5）, imaging Object lens（6）And convergent lens（10）90% is more than to wavelength 1000nm ~ 1600nm light transmission rate；The image-forming objective lens（6）Work Distance ＞ 10mm, numerical aperture are more than 0.2.
- 6. tumor-microvessel imager according to claim 1, it is characterised in that：The near-infrared fluorescent optical filter（9） To be more than 90% to wavelength 1400nm ~ 1500nm light transmission rate, and it is more than 3 bandpass filter to cutoff wavelength OD.
- 7. tumor-microvessel imager according to claim 1, it is characterised in that：The pin hole（11）Positioned at convergent lens （10）Focal plane at, its size is convergent lens（10）Airy disc size；The detector（12）Against pin hole（11）Place, And the photomultiplier of indium gallium arsenic material should be used.
- 8. according to the tumor-microvessel imager described in claim 1-7 any one, it is characterised in that：The biological specimen It is full in advance in blood vessel to have near-infrared quantum dots of the fluorescence emission wavelengths for 1300nm ~ 1500nm.
- A kind of 9. tumor-microvessel imaging method, it is characterised in that：Data processing image display（14）Point by point scanning is visited The signal of survey is converted into the gray value of single pixel, obtains single layer image after its order is arranged, it is micro- so to obtain tumour The individual layer sectional drawing of blood vessel.
- A kind of 10. tumor-microvessel imaging method, it is characterised in that：Data processing image display（14）Point by point scanning is visited The signal of survey is converted into the gray value of single pixel, obtains single layer image after its order is arranged, then sample stage（7）Rise one Fixed height, repeats scanned samples and obtains next layer of single layer image, so circulate certain number of plies, these sequence images are carried out Three-dimensional reconstruction is the three-dimensional structure image that can obtain tumor-microvessel.
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| CN108498079A (en) * | 2018-05-08 | 2018-09-07 | 翰飞骏德（北京）医疗科技有限公司 | Recognition methods and the equipment of glimmering Control of Environment Pollution by Light medium vessels, lymphatic vessel and lymph node |
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